Brassica Validation Study
Protocol Issues
• Draft protocol timing/development• 2 Study Directors• US GLPS for Field Portion (GLP “Light”)
Protocol Issues• 8 crops of interest in one study• 8 crop observations on each app date and
tracking during crop cycles.• 1 day PHI• Sampling to occur on the same date for all crops• All Untreated crop samples collected first• 8 each Part 7A Sample Collection & Storage
Forms• 6 different Sampling Fractions by crop• 2 Minimum Sample Sizes by crop
Protocol Issues
• 2 Application Types in the same study• Directed Foliar Applications #1 & 2 of 5 total• Foliar Broadcast Applications #3,4,&5• Field Tank Mixes of 3 Test Substances• APP.#1 = Provado 1.6F• APP.#2 = Provado +Switch 62 WDG• APP.#3,4,&5 = Provado + Switch + Spinosad
Protocol Issues
Protocol Issues
Protocol Issues
Protocol Isqsues
Protocol Issues
Protocol Issues
Protocol Issues
Protocol Issues
Protocol Issues
•
Study Conduct Issues• Days to Harvest:• Broccoli /85 days • Cabbage / 80-90 days• Kale /80-85 Days• Chinese Napa Cabbage / 65days• Kohlrabi /50-60 days• Bok Choy / 55 days• Mustard Greens /35days• Chinese Broccoli /44 days
Study Conduct Issues
• Broccoli,Cabbage, and Kale:Greenhouse plant date= 9/05/08Field transplant date = 10/15/08• Napa cabbage,Kohlrabi,Bok Choy,Mustard
Greens,Chinese BroccoliGreenhouse plant date=9/22/08Field transplant date= 11/17/08• Test Substance Application #1 =11/25/08 to all
of the crops
Study Conduct Issues• 11 other studies at the same time including a
Canadian trial(my first one)• Weather impacts/possibility of winds and our
annual rainfall occurring near the end of the study
• Whitefly and worm control on the untreated plots/whitefly overwinters on broccoli
• What are the good growing practices for Chinese Broccoli etc in my growing area?
• Water salinity impacts on specialty crops { 1ton of salts in 1 acre foot of irrigation water};ph =8
Study Conduct Issues
Study Conduct Issues
Study Conduct Issues
Study Conduct Issues
Study Conduct Issues
• Impacts of soil salinity and type (silty clay loam) on specialty crops
• Accurate measurement of large carrier volumes for spray solutions.
• Adequate freezer space to hold samples for all of the studies
• Timely shipping of samples to EPA lab during the Christmas and New Years holiday
Study Conduct Issues
Study Conduct Issues
Study Conduct Issues
Study Conduct Issues
Study Conduct Issues
Study Conduct Issues
Study Conduct Issues
Study Conduct Issues
Thank you to : Dr. Bill Barney. Marylee Ross Stephen Flanagan BeckySisco Jim McFarland & Martin Beran Keithly Williams Seed Company