Brassica Validation Study

Protocol Issues

• Draft protocol timing/development• 2 Study Directors• US GLPS for Field Portion (GLP “Light”)

Protocol Issues• 8 crops of interest in one study• 8 crop observations on each app date and

tracking during crop cycles.• 1 day PHI• Sampling to occur on the same date for all crops• All Untreated crop samples collected first• 8 each Part 7A Sample Collection & Storage

Forms• 6 different Sampling Fractions by crop• 2 Minimum Sample Sizes by crop

Protocol Issues

• 2 Application Types in the same study• Directed Foliar Applications #1 & 2 of 5 total• Foliar Broadcast Applications #3,4,&5• Field Tank Mixes of 3 Test Substances• APP.#1 = Provado 1.6F• APP.#2 = Provado +Switch 62 WDG• APP.#3,4,&5 = Provado + Switch + Spinosad

Protocol Issues

Protocol Issues

Protocol Issues

Protocol Isqsues

Protocol Issues

Protocol Issues

Protocol Issues

Protocol Issues

Protocol Issues

•

Study Conduct Issues• Days to Harvest:• Broccoli /85 days • Cabbage / 80-90 days• Kale /80-85 Days• Chinese Napa Cabbage / 65days• Kohlrabi /50-60 days• Bok Choy / 55 days• Mustard Greens /35days• Chinese Broccoli /44 days

Study Conduct Issues

• Broccoli,Cabbage, and Kale:Greenhouse plant date= 9/05/08Field transplant date = 10/15/08• Napa cabbage,Kohlrabi,Bok Choy,Mustard

Greens,Chinese BroccoliGreenhouse plant date=9/22/08Field transplant date= 11/17/08• Test Substance Application #1 =11/25/08 to all

of the crops

Study Conduct Issues• 11 other studies at the same time including a

Canadian trial(my first one)• Weather impacts/possibility of winds and our

annual rainfall occurring near the end of the study

• Whitefly and worm control on the untreated plots/whitefly overwinters on broccoli

• What are the good growing practices for Chinese Broccoli etc in my growing area?

• Water salinity impacts on specialty crops { 1ton of salts in 1 acre foot of irrigation water};ph =8

Study Conduct Issues

Study Conduct Issues

Study Conduct Issues

Study Conduct Issues

Study Conduct Issues

• Impacts of soil salinity and type (silty clay loam) on specialty crops

• Accurate measurement of large carrier volumes for spray solutions.

• Adequate freezer space to hold samples for all of the studies

• Timely shipping of samples to EPA lab during the Christmas and New Years holiday

Study Conduct Issues

Study Conduct Issues

Study Conduct Issues

Study Conduct Issues

Study Conduct Issues

Study Conduct Issues

Study Conduct Issues

Study Conduct Issues

Thank you to : Dr. Bill Barney. Marylee Ross Stephen Flanagan BeckySisco Jim McFarland & Martin Beran Keithly Williams Seed Company