www.westcoastseparations.com +1-(805) 504-7767 3201 Corte Malpaso, Unit 310, Camarillo, CA 93012
Westcoast
Separations, LLC “Building relationships with reliable and
cost-effective separation services”
Due to the lack of efficient HPLC method for the analysis of the post purification of the individual
cannabinoids, we developed a fast and efficient method to separate the mixture and in turn use for
analysis.
HPLC separation of 7 cannabinoids using Agilent XDB column (50x4.6 mm), 1.8 µM. Mobile
Phase: A – 0.1% TFA in Water; B – 0.1% TFA in MeCN at 1 ml/min (0, 25%B); (4 min,
100%B); (6.5 min, 100%B); (6.7 min, 25%B). Wavelength: 220-nm
12
3
4
5
6
7
8
1 – THC
2 – Impurity
3 – CBDA
4 – CBG
5 – CBD
6 – CBN
7 – THCA
8 - CBC
min0 1 2 3 4 5 6