Workflow de Ion Torrent

8/4/2019 Workflow de Ion Torrent

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astest next-gen workfow10X more throughput

astest-selling sequencerall in six monthsIon Personal Genome Machine™ Sequencer



Ion 316™ sequencing chiptenold increase in throughput ater just six months

Semiconductor Sequencing

Semiconductor technology has transformed every industryit has touched, from computing to photography to music.And now it’s transforming the life sciences. The IonPersonal Genome Machine™ sequencer is powered by aninnovative semiconductor chip which makes it faster, easierto use, and more accessible than any light-based solution.

Experience the speed, simplicity, and scalability of semi-conductor sequencing with the Ion PGM™ sequencer.

Ion Torrent has pioneered an entirelynew approach to sequencing. Insteadof using light as an intermediary tosequence DNA, Ion Torrent usessemiconductor technology and simplechemistry. Ion Torrent translateschemical signals into digital informa-tion, much as a CMOS chip translatesphotons into pixels.

Ion semiconductor sequencing bringsthe entire design, fabrication andsupply chain infrastructure of thesemiconductor industry—a $1 trillioninvestment—to bear on the challengeof sequencing DNA. Just as themicroprocessor enabled desktopcomputing to displace the mainframe,Ion Torrent semiconductor technologywill inevitably democratize sequencing,putting it within the reach of any lab.

ION TORRENT S EMIC ONDUC TOR S EQUEN

01

“The biggest advantage [of thPGM] from my point of viewa public health ofcial is thait’s speedy, and speed is whis needed at the moment.”PROF.DR.MED.DAGHARMSEN

UNIVERSITY HOSPITAL, MÜNSTER, GERMANY



3.5 H O U R S

0.5 H O U R S

3 H O U R S

Onlyminutes ofhands-on time 2 H

O U R S

Underone hour forshort reads

T E T E IC CT E E C T E T E IC CT E EI T E T E IC CT E EI T E T E IC CT E

WORKFLOW TIMES>

astestnext-genworkfow


03

01

ConstructLibrary

The new Ion Xpress™FragmentLibrary Kit offers a novel enzymaticfragmentation process that has reducedlibrary preparation to 3.5 hours, 60percent faster than six months ago.

+ ION KI T S + I ON ONE-T OUCH™ S Y S T EM + ION P GM™ S EQUENCER + T ORR ENT S ER V ER

Ion Torrent’s single-day workowis now the fastest in next-generationsequencing, at under eight hours.The time was reduced approximatelythreefold in just six months, thanksto key breakthroughs at each stageof the workow:

02

PrepareTemplate

The Ion OneTouch™ System* fortemplate preparation, shipping in thethird quarter, has added a componentto automate enrichment, the IonOneTouch™ ES.* Now the entire templatepreparation process is fully automatedand can be completed in three hours,70 percent faster than six months ago.

03

RunSequence

The new Ion 316™ chip has a two-hourrun time for 100 base pair reads, justlike the Ion 314™chip. Run times arereduced to less than one hour whensequencing short reads.

04

AnalyzeData

The new Torrent Suite 1.4 analysissoftware cuts analysis time to just30 minutes on the Ion 314™ chip andone hour on the Ion 316 ™ chip, whilealso delivering a signicant improve-ment in base-call accuracy.

“[The PGM] takes theshortest time to generatgenomic data.”JUNJIEQIN

BGI, CHINA



nothing gets better asterthan Ion semiconductor sequencing


05

Six months to 10-old improvement

In the six months since the Ion PGM™

sequencer launched, its throughput hasincreased tenfold, its workow hasbecome the fastest in the industry, andit’s now the fastest-selling sequencerin the world, with placements in morethan 40 countries. In the second halfof the year you’ll see more of the same.

Read lengths will double to 200 basepairs and throughput will increaseby tenfold—again.

All of these advances are a direct resultof Ion’s semiconductor sequencingtechnology and native chemistry, whichmake sequencing faster, simpler, andmore scalable than ever before.

More Throughput, Better Value

The scalability of Ion semiconductorsequencing will enable a hundredfoldincrease in throughput this year.The new Ion 316™ chip has 100 Mbof throughput, tenfold more than theoriginal Ion 314™chip. The Ion 318™

chip, available in the fourth quarter,will deliver 1 Gb of throughput. Each

of the three chips will work on theIon PGM™ sequencer, giving usersthe exibility to buy only as muchthroughput as they need for anexperiment, from 10 Mb to 1 Gb.

Ion semiconductor sequencing offersexceptional value as well as perfor-mance. The Ion 314™ chip is nowavailable for just $99 USD, whichenables the sequencing community todevelop novel methods and applica-tions. Ion Torrent is able to lower theprice because of the signicant salesvolume generated by the Ion 314™ chipand the economies of scale inherent insemiconductor manufacturing.

Better Accuracy

The Ion PGM™ system’s accuracy hasimproved by an order of magnitudesince it was launched six months ago,as a result of the sy stem’s speed andsimplicity. Ion Torrent has capitalizedon the inherent accuracy of its simplesequencing chemistry, achieving moresignal, better signal processing and

improved base calling. Accuracyimproved quickly in part because theIon PGM™ sequencer’s two-hour runtimes enable Ion Torrent R&D teams toquickly test and rene ideas. Improve-ments in accuracy will help Ion Torrentreach 200 base pair reads in the secondhalf of this year and 400 base pair readsin 2012, making many new applicationspossible for researchers.

More New Products

Additional new products now availableinclude an early-access version of theIon DNA Barcoding 1-16 Kit* formultiplexed sequence analysis, the IonLibrary Quantitation Kit for TaqMan®-based quantitation of Ion DNA libraries,and the Ion Sphere™Quality Control Kitfor template bead quality control.

The Ion PGM™ Sequencerin Customers’ Hands

Scientists turned to the Ion PGM ™

sequencer to identify a deadly strainof E. coli in Europe in June because thesystem’s two-hour run time was fasterthan anything else on the market.Scientists at BGI sequenced thebacterium within three days, and ateam at the University of Münstersequenced the strain and developed aeld-deployable test in just ve days.

Scientists around the world also havereported exceptional throughputresults with their rst runs on the Ion316™ chip. Early-access users at BaylorCollege of Medicine recently broke IonTorrent’s internal record for highestoutput, after reporting 287 Mb from asingle Ion 316™chip run, and the BroadInstitute reported up to 250 Mb. Mostrecently, the Wellcome Trust SangerInstitute reported that one of the rstruns of an Ion 316™ chip on their Ion

PGM™

sequencer produced a totalyield of 194 Mb.

ION SEMICONDUCTOR SEQUENCINGRAPIDLY IMPROVING ACCURACY

†w ww.lifetechnologies.com/ion314data

REMARKABLE SCALABILITY:10MB TO 1GB IN ONE YEAR (100X)

314

JAN:2011

10 Mb

100 bp

CHIP

DATE

OUTPUT

READ LENGTH

316

JUL:2011

100 Mb

100 bp

318*

Q4:2011

1 Gb

200 bp



To fnd out more about how semiconductor sequencing

technology works and to hear what scientist are doing with

the Ion PGM™ sequencer, join the Ion Community at:

http://ioncommunity.iontorrent.com

Register or the Lie Grand Challenges or your chance

to win $1 million, at:

www.lietechnologies.com/grandchallenges

*The content provided herein may relate to products that have not been ofcially released and is subject to change without notice.

FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAPEUTIC OR DIAGNOSTIC USE.© 2011 Lie Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property o Lie Technologies Corporationor their respective owners. TaqMan is a registered trademark o Roche Molecular Systems, Inc.

CO31580 0711

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Workflow de Ion Torrent