scalabilitysimplicityspeed

Ion Personal Genome Machine™ SequencerThe Chip is the Machine™

Discover Ion TorrentThe Ion Personal Genome Machine™ (PGM™) sequencer is more simple, cost-effective, and scalable than any other sequencing technology. The Ion PGM™ sequencer is a benchtop system utilizing groundbreaking and disruptive semiconduc-tor technology that enables rapid and scalable sequencing.

Ion Torrent™ technology uses a massively parallel array of proprietary semiconductor sensors to perform direct, real-time measurement of the hydrogen ions produced during DNA replication. A high-density array of wells on the Ion semicon-ductor chips provides millions of individual reactors, and integrated fluidics allow reagents to flow over the sensor array. This unique combination of fluidics, micromachining, and semiconductor technology enables the direct translation of genetic information (DNA) to digital information (DNA sequence), rapidly generating large quantities of high-quality data.

The Ion PGM™ sequencer, along with Ion semiconductor sequencing chips, Ion reagent kits, and the Torrent Server/Torrent Suite software, allow Ion Torrent to deliver a cutting-edge sequencing solution.

Scalabilitymassively scalable semiconductor technology

Simplicitynatural biochemistry, no optical components

Speedruns in about two hours, not days or weeks

Discover scalabilitysemiconductor scaling

• Leverages $1 trillion semiconductor industry investment

• Scales by density: 40 cumulative years of Moore’s Law

• Scales by volume: 400 standard semiconductor foundries from which to choose

Discover simplicitysimple chemistry

• Simplest sequencing chemistry: hydrogen ion detection (pH meter)

• Natural nucleotides: no enzymatic cascade, no fluorescence, and no chemiluminescence

• Low-cost, standard reagents

Discover speeddirect, real-time detection technology

• Sequencing accessible to new applications and markets

• Short run-time supports fast research cycle and faster time to publication

• The Chip is the Machine™: no optics, no light, no scanning

Sequencing and analysis pipeline

The Ion PGM™ workflow consists of a number of standard elements—creation of a DNA or RNA library and a simple, automated PCR process for amplification of templates onto proprietary Ion Sphere™ particles, followed by sequencing on the Ion PGM™ sequencer.

With the completion of a run, data automatically transfers to the dedicated Torrent Server that runs Torrent Suite software. Here, raw Ion signals are con-verted to base calls and stored in industry-standard SFF, FASTQ, or BAM files. These data can be processed by a variety of commercially available software packages for applications such as targeted resequencing, RNA-Seq, ChIP-Seq, or de novo genome assembly.

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For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.© 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Printed in the USA. CO01229 1011

Ordering informationDescription Cat. No.Personal Genome Machine™ SystemIon PGM™ SystemIncludes PGM™ sequencer (4462917) and Torrent Server (4462918)

4462921

Ion OneTouch™ SystemIon OneTouch™ System 4470001Service contractsAB Assurance Plan ZG11SCIONPGMSYSRapid Exchange Program ZGEXSCIONPGMSYSSemiconductor sequencing chipsIon 314™ Chip Kit (8 Chips) 4462923Ion 316™ Chip Kit (4 Chips) 4466616Ion 316™ Chip Kit (8 chips) 4469496Library kitsIon Xpress™ Plus Fragment Library Kit (10 reactions) 4471269Ion Plus Fragment Library Kit (10 reactions) 4471252Ion Total RNA-Seq Kit (12 reactions) 4466666Ion Total RNA-Seq Kit (48 reactions) 4468200Ion AmpliSeq™ Cancer Panel Kit 4472395Ion TargetSeq™ Custom Enrichment Kit 100 kb-500 kb (96 enrichments) A14230Ion TargetSeq™ Custom Enrichment Kit 500 kb - 2 Mb (96 enrichments) A14229Ion TargetSeq™ Custom Enrichment Kit 2 Mb - 10 Mb (96 enrichments) A14228Ion Xpress™ Barcode Adaptors 1-16 (10 sets of 16 libraries) 4471250Reagent kits100 bp read lengthIon Xpress™ Template Kit (10 reactions) 4469001Ion OneTouch™ Template Kit (10 reactions) 4468660Ion Sequencing Kit (8 reactions) 4468997200 bp read lengthIon Xpress™ Template 200 Kit (10 reactions) 4471253Ion Sequencing 200 Kit (8 reactions) 4471258Control kits (use for both 100 and 200 bp read lengths)Ion Control Materials 200 Kit (30 reaction of input gDNA, 10 reactions for DNA control library and 3 reactions for sequencing controls)

4471249

Ion Sphere™ Quality Control Kit 4468656Ion Library Quantitation Kit 4468802* Throughput calculated with Escherichia coli DH10B genome, using 100 base pair sequencing chemistry and a 100 bp read length PGM™ sequencer configuration. † The content provided herein may relate to products that have not been officially released and is subject to change without notice.

SpecificationsIon 314™ Chip Ion 316™ Chip Ion 318™ Chip †

Scale 1.3 million wells 6.3 million wells 11 million wells

Data output Starting at 10 Mb of high-quality sequence*

Starting at 100 Mb of high-quality sequence*

Starting at 1 Gb of high-quality sequence*

Read length Up to 200 base pairs

Files produced SFF, FASTQ, BAM

Reagents Complete set of reagents, including library, template, and sequencing kits

Applications Targeted resequencing, small genome sequencing, RNA-Seq, ChIP-Seq, copy number variation, methylation, paired-end sequencing, and mate-paired sequencing