Wireless bio-microsystems platform based on Microfluidic ......Miled and Gosselin 2013 Biomedical Microsystems Laboratory Page 11 6 7.6 8 9.6 9 0 2 4 6 8 10 12 5 10 15 20 25 ACF volume

Wireless bio-microsystems platform based on Microfluidic and Microelectronics hybrid Integration

Amine Miled and Benoit Gosselin Biomedical Microsystems Lab

Electrical and Computer Engineering Department Laval University, Quebec City, Qc

Toronto November 25, 2013

CMC Microsystems - MIP User Group Meeting

Miled and Gosselin 2013 Page 2 Biomedical Microsystems Laboratory

Outline

1.  MIP configuration at Laval University 2.  MIP present applications

3.  MIP Future Application 4.  Available ressources


1. MIP Configuration at Laval University

PXI

Syringe pump

Stage

Computer with labVIEW

interface

Camera

Pressure sensor

MIP test bench configuration at Laval University

Microscope


PXI Cards : •  PXIe-8135 embedded

controller •  PXI-5114 Digitizer •  PXI-4130 Power SMU •  PXI-7854R R Series •  PXI-4110 Programmable

DC supply •  ProLight-1101 FPGA

Module

Other components :

•  Harvard Apparatus Pump 11 Pico Plus Elite

•  Motor Driver

•  ADSYS Microscopic Imaging Research Station (MIRS)

•  Objectives x50, x20, x5



Syringe Pump

Flow Meter

Pressure Sensor

Controller

Camera

Digitizer

Light Source

Stage

Design Under Test

PXI Embedded Computer

Source Management

Unit



2. MIP Present Applications

•  Only the pre-designed example VIs have been tested

–  GigE Camera Viewer VI –  FPGA Camera Viewer VI

Lightening problem with MIP: Misalignment

CMOS Chip Tests


•  Features of a microelectronic device are clear using GigE camera even with ambient light

–  However, a wide view of standard chip size was not possible with default objectives

–  We recommend a smaller objective

•  Lightening problem due to misalignment even with low objective (x5)

CMOS Chip Tests (Cont’d)



•  An ultra-wideband CMOS 180 nm transceiver for low-power bio-monitoring applications

CMOS Chip Tests (Cont’d)



Inductive array

§  Smart research tools to study freely moving animals Multi-Technology Bio-Microsystems Tests



§  Need for a miniaturized system to address smaller animals §  Envisioned microsystem:

  128 bio-monitoring channels at 20 ksps per ch.

  > 16 optical/electrical stimulation channels

  Embedded microfluidic sensor   Dimensions < 1 cm2, weight < 1g,

transmission range > 2 meters

Wideband antenna Secondary coil

CMOS SOC

Biocompatible coating •  Matching network

•  Electrodes •  Fibres

Multi-Technology Bio-Microsystems Tests (Cont’d)



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0

2

4

6

8

10

12

5 10 15 20 25

Freq

uenc

y kH

z

ACF volume (ul)

70.5 63.3

48.2

25.36 22.4

0 10 20 30 40 50 60 70 80 90

100

0 2 3 5 9 % o

f dis

inte

grat

ion

of E

lect

rode

Time (min)

Effect of aCSF* within 1.8 ml of DW* with 40 µl of 1 µm microspheres on electrode**

Limits:

Starting from 30 µl of aCSF, electrode disintegration becomes more considerable

* DW: Distilled water , aCSF: Artificial cerebrospinal fluid


**Miled, M.A; Sawan, M., "Electrode Robustness in Artificial Cerebrospinal Fluid for Dielectrophoresis-Based LoC", IEEE Int. Conf. Engineering in Medicine and Biology, Sep. 2012 (invited)



MIP test bench configuration Comparison*

PXI

Syringe pump

Stage

Computer with labVIEW interface

Camera

Pressure sensor

Microscope

!

Microscope

Camera

Data acquisition system

3 channel oscilloscopes

Syringe and micropump


Microfluidic microchannels

CMOS chip

Power supplies

Actel fusion FPGA

CMOS/uFluidic connections

*Miled, M.A. ; Sawan, M.; "Dielectrophoresis-Based Integrated Lab-on-Chip for Nano and Micro-Particles Manipulation and Capacitive Detection", IEEE transaction on Biomedical Circuits and Systems, Pages: 120-132, Vo. 6, No. 2, 2012



Self Designed MIP test bench configuration* !

Microscope

Camera

Data acquisition system


Syringe and micropump


Microfluidic microchannels

CMOS chip

Power supplies

Actel fusion FPGA

CMOS/uFluidic connections

*Miled, M.A. ; Sawan, M.; "Dielectrophoresis-Based Integrated Lab-on-Chip for Nano and Micro-Particles Manipulation and Capacitive Detection", IEEE transaction on Biomedical Circuits and Systems, Pages: 120-132, Vo. 6, No. 2, 2012


!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

Metal stiffener

Plexiglass

Interposer PCB

284 electrode connections

Micromanipulator

Microtube

Epoxy

Extendable microfluidic packaging for prototyping purpose*

14 / 62


*Miled, M.A.; Sawan, M.;"High Throughput Microfluidic Rapid Prototyping Packaging Methods for DEP manipulations" , the Journal of Visualized Experiments, In press


References ……

Top glass

PDMS

Bottom glass

Tubes

Syringe

Epoxy

PDMS

Syringe

System set-up comparaison: (a) Using epoxy, and (b) using PDMS interconnect layer*

3. MIP Future Applications

In-channel Liquid Flow Monitoring

*Miled, M.A.; Sawan, M.;"High Throughput Microfluidic Rapid Prototyping Packaging Methods for DEP manipulations" , the Journal of Visualized Experiments, In press

Miled and Gosselin 2013 Page 16 Biomedical Microsystems Laboratory 16

(a) L-shape electrodes (b) Mixing electrodes*

(a) (b)

The detected variation of capacitance is 1.06 pF with PCB board based circuit**


In-channel Liquid Flow Monitoring (Cont’d)

* Miled, M.A.; El-Achkar, C.M. ; Sawan, M., "Low-voltage dielectrophoretic platform for Lab-on-chip biosensing applications", IEEE Int. NEWCAS, pp. 389 -392, May. 2010.

** Miled, M.A.; Sawan, M., "Reconfigurable dielectrophoretic device for neurotransmitters sensing and manipulation ", IMS3TW 2009, pp. 1 - 4, Jun. 2009


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Flat dropTop glass plate

Bottom glass plate

Ef Ef

Circular dropTop glass plate

Bottom glass plate

Ef

Flat dropTop glass plate

Bottom glass plate

Ef Ef

Circular dropTop glass plate

Bottom glass plate

Ef

GND

Capacitive sensor

Epoxy Particle

Bottom glass plate

Detection area Manipulation area

High sensitivity capacitive detector

Monitoring electrical circuit

Detection primary results

Electrical field propagation in the

LoC

Manipulation principle

17


High Throughput Microfluidic Design Test


Available ressources

In addition to CMC ressources:

https://wiki.gel.ulaval.ca/index.php/EmSYSCAN


Financial Support

Oxy'nov Inc.

Documents

Wireless bio-microsystems platform based on Microfluidic ......Miled and Gosselin 2013 Biomedical Microsystems Laboratory Page 11 6 7.6 8 9.6 9 0 2 4 6 8 10 12 5 10 15 20 25 ACF volume