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Wild Type Drosophila. Ommatidium. 8 Photoreceptor cells (R1-R8) 4 Cone cells (secrete lens) 8 Pigment cells. Wild Type Eye Phenotype. Eyeless Mutant Phenotype (hypomorphic allele). Paired Domain Protein. DNA. Figure 1A. Gal4 Enhancer-Trap Method. LacZ. No Transcription of LacZ. - PowerPoint PPT Presentation
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Wild Type Drosophila
Ommatidium
8 Photoreceptor cells (R1-R8)4 Cone cells (secrete lens)8 Pigment cells
Wild Type Eye Phenotype
Eyeless Mutant Phenotype (hypomorphic allele)
DNA
Paired Domain Protein
Figure 1A
LacZ
No Transcription of LacZ
Gal4 Enhancer-Trap Method
Figure 1A
LacZ
Enhancer that controls expression in specific tissues
Gal4 Enhancer-Trap Method
Figure 1A
LacZ
Tissue-specific Expressionof Gal4
Gal4 Enhancer-Trap Method
Tissue-specific Expressionof LacZ
Figure 1
Eye-Antennal disc wing disc leg disc
Figure 1A
LacZ
Gal4 Enhancer-Trap Method
Enhancer that controls expression in imaginaldisc cells
Transcription of LacZ in imginal disc cells
Figure 1A
Enhancer that controls expression in imaginaldisc cells
Transcription of eyeless in imginal disc cells
Figure 2
Figure 3
DNA
Paired Domain Protein
Drosophila ey, mouse Pax-6 (sey) and Human Pax-6 (Aniridia) genesare ~ 94% similar in Paired domain
Enhancer that controls expression in imaginaldisc cells
Transcription of Pax-6 in imginal disc cells
Pax-6
Ectopic Expression of Mouse Pax-6 Gene
Figure 5
Ectopic Expression of Mouse Pax-6 Gene
Aniridia -- Human Pax-6 Mutation
Absence of the iris
Xenopus Oocyte
Animal Pole
Vegetal Pole
Xwnt-11 mRNA Localization
Xwnt-11 is Drosophila wingless homogue
Normal
Ventralized
Embryo Grafting Experiment
Goosecoid ProteinExpression
Embryo Grafting Experiment
InjectmRNAs
ForChordin etc.
Formation of ventral structures
Normal
Dorsalized
