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Wild Type Drosophila Slide 2 Ommatidium 8 Photoreceptor cells (R1-R8) 4 Cone cells (secrete lens) 8 Pigment cells Slide 3 Wild Type Eye Phenotype Slide 4 Eyeless Mutant Phenotype (hypomorphic allele) Slide 5 DNA Paired Domain Protein Slide 6 Figure 1A LacZ No Transcription of LacZ Gal4 Enhancer-Trap Method Slide 7 Figure 1A LacZ Enhancer that controls expression in specific tissues Gal4 Enhancer-Trap Method Slide 8 Figure 1A LacZ Tissue-specific Expression of Gal4 Gal4 Enhancer-Trap Method Tissue-specific Expression of LacZ Slide 9 Figure 1 Eye-Antennal disc wing disc leg disc Slide 10 Slide 11 Figure 1A LacZ Gal4 Enhancer-Trap Method Enhancer that controls expression in imaginal disc cells Transcription of LacZ in imginal disc cells Slide 12 Figure 1A Enhancer that controls expression in imaginal disc cells Transcription of eyeless in imginal disc cells Slide 13 Figure 2 Slide 14 Figure 3 Slide 15 DNA Paired Domain Protein Drosophila ey, mouse Pax-6 (sey) and Human Pax-6 (Aniridia) genes are ~ 94% similar in Paired domain Slide 16 Enhancer that controls expression in imaginal disc cells Transcription of Pax-6 in imginal disc cells Pax-6 Ectopic Expression of Mouse Pax-6 Gene Slide 17 Figure 5 Ectopic Expression of Mouse Pax-6 Gene Slide 18 Aniridia -- Human Pax-6 Mutation Absence of the iris Slide 19 Slide 20 Slide 21 Slide 22 Slide 23 Xenopus Oocyte Animal Pole Vegetal Pole Slide 24 Xwnt-11 mRNA Localization Xwnt-11 is Drosophila wingless homogue Slide 25 Normal Ventralized Slide 26 Embryo Grafting Experiment Slide 27 Slide 28 Goosecoid Protein Expression Slide 29 Embryo Grafting Experiment Inject mRNAs For Chordin etc. Slide 30 Slide 31 Formation of ventral structures Slide 32 Slide 33 Normal Dorsalized Slide 34