What’s New in LAB & Life Sciences Jun/Jul 2014

Cleanrooms, pipettes and baking lenses

JUNE/JULY 2014 VOL.25 NO.2PP100008671

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lab & LIFE SCIENCES

JUNE/JULY 2014

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04 Bake your own microscope lens

06 Controlling controlled and sterile

environments

14 How application and workflow

affect pipette selection

21 Building bone marrow-on-a-chip

26 Harvard scientists create the

hemihelix

29 E-commerce in the lab

32 Exotic particle confirmed by CERN

34 My Lab

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Australian scientists have invented a simple way of making a high-powered lens that costs less than a cent and can transform a smartphone into a high-resolution microscope. The transformation is made by attaching the tiny polymer lens onto the camera lens of a phone.

Bake your own microscope lens

The lens fabrication technique was invented by Dr Steve Lee from the Australian National University (ANU) Research School of Engineering, who

collaborated with Dr Tri Phan from the Garvan Institute of Medical Research to find ways to transform the lens into a medical imaging tool. Their work has been published in the Optical Society journal Biomedical Optics Express.

Many conventional lenses are made by grinding and polishing a flat disk of glass into a particular curved shape. Others are made by pouring gel-like materials moulds. With the new method, all that’s needed is an oven, a microscope glass slide and a common, gel-like silicone polymer called polydimethylsiloxane (PDMS), which is used for contact lenses.

First, drop a small amount of PDMS onto the slide. Then bake it at 70°C to harden it, creating a base. Then, drop another dollop of PDMS onto the base and flip the slide over. Gravity pulls the new droplet down into a parabolic shape. Bake the droplet again to solidify the lens.

“It’s very easy to do - in fact, I think that anyone at home could do it,” said Dr Lee. “It costs around less than a cent to make a single lens.”

Dr Lee admitted that the first droplet lenses were made by accident and he nearly threw them away.

“I happened to mention them to my colleague Tri Phan, and he got very excited,” Dr Lee said.

“So then I decided to try to find the optimum shape, to see how far I could go. When I saw the first images of yeast cells I was like, ‘Wow!’”

Dr Lee found that by successively adding small amounts of fluid, creating a lens just a few millimetres thick, they could reach a magnifying power of up to 160 times with an imaging resolution of four micrometres - two times lower in optical resolution than many commercial microscopes, but more than three orders of magnitude lower in cost.

Dr Lee and his team worked with Dr Phan to design a lightweight 3D-printable frame to hold the lens, along with a couple of miniature LED lights for illumination and a coin battery. The attachment turns a smartphone camera into a dermascope - a tool to diagnose skin diseases like melanoma - which can cost $500 or more. The phone version costs just $2.

The tiny microscope has a wide range of potential uses. Dr Lee envisions that the lenses could be used by biologists to study cells in vivo; by farmers to identify pests out in their fields; or in classrooms for educational purposes. Their simplicity and low cost make them especially suitable for the third world, he added.

So far, the researchers can’t make lenses much bigger than half an inch in diameter. The team is now refining the process to make lenses as large as two inches and increasing the lens’s optical performance.

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All licensed drug manufacturers must comply with current Good Manufacturing Practice regulation. Cell and tissue, radiopharmacy and cytotoxic manufacturers also fall under the same scrutiny as do some research facilities. Compliance must be demonstrated at every stage before a drug or human medicinal product can be released to market. Automated environmental monitoring and biological monitoring systems provide effective and reliable methods for monitoring of viable and non-viable particles in Grade A/B cleanrooms along with other critical environmental conditions such as pressure, relative humidity and temperature.

Controlling controlled and sterile environments

WHAT’S NEW IN LAB & LIFE SCIENCES - June/July 2014 7www.LabOnline.com.au

However, keeping a cleanroom clean is not easy. It will be at its zenith of ‘cleanliness’ before any equipment or op-

erators are introduced into it - but let’s face it, even though the environment may be sterile, without equipment and workers it will be useless.

As people and equipment enter the cleanroom they bring particulate matter with them. Gowning up and the use of smooth surfaces on process equipment will help reduce the build-up of particles but equipment with moving parts and the operators still generate considerable quantities of particles.

Sources of contamination may include dead skin and hair, cosmetics and cloth-ing, and bacteria. Independent tests have shown that the average human gener-ates around 500,000 particles/minute. That figure reduces by use of suitable attire, gowns, masks, gloves, and hair and beard coverings, but particles are still generated and an addition of a new contaminated product or ingression from an operating environment can further increase the particulate contamination.

There are two types of particle rep-resentations present in our environment - viable and non-viable particles. Viable particles are the ones that are feared most in pharmaceutical, medical device and biotech cleanrooms as they contain living organisms.

Along with other critical environmen-tal conditions such as pressure, relative humidity and temperature control, auto-mated environmental monitoring (EMS) and biological monitoring systems (BMS) can provide an effective and reliable method for the monitoring of viable and non-viable particles in Grade A/B cleanrooms.

An environmental monitoring sys-tem (EMS) contains particle sensors mounted in critical areas such as along a filling machine or inside a biological safety cabinet that detect non-viable particles. Other environmental sensors can be included in the EMS monitoring such as room pressure, humidity and

temperature. The data is sent back to a software system that is controlled and monitored by the operator. Any out-of-specification alarms will trigger an email/SMS notification or activate local alarms and flashing indicators within the cleanrooms.

Inside a cleanroom, the particulate contamination in the environment can be controlled using HEPA filters - high-efficiency particle air filters. Depending on the classification of the cleanroom, the number of air changes per hour can be configured. That environment is tested and classified based on the validation work carried out.

Particle counters and air samplers are used to monitor cleanrooms and sterile controlled environments. Particle coun-ters detect the size and concentration of particles present. Air samplers detect what types of particles are present by capturing them on a media plate.

Grade A sterile environments moni-tor continuously during production us-ing environmental monitoring systems combined with automated or real-time biological monitoring systems.

The control room can be located off the cleanroom, which keeps the monitor-ing operator out of the sterile area. The BMS also has sampling heads located along the filling machine or inside LAF or biological safety cabinets. Before the process begins, the sampling heads are loaded with 90 mm Petri dishes containing a media - the most common media being TSA. One system on the market cleverly uses barcoding so the BMS software knows the 4 Ws - Who, What, When and Where. The 4 Ws ask: WHO loads the Petri dish; WHAT dish is loaded; WHEN is the dish loaded; and WHERE is the dish loaded. After processing, the 4 Ws are stored in the software: WHO unloads the sample; WHAT sample is unloaded; WHEN was the sample unloaded; and WHERE is the sample unloaded. The sample is then incubated and the colony forming units (CFUs) counted, and the data is down-loaded into the software so a complete


THERE ARE SOME SOFTWARE PACKAGES ON

THE MARKET THAT COMBINE THE EMS AND THE

BIOLOGICAL MONITORING ONTO ONE COMPLETE

PACKAGE ALLOWING FOR REAL-TIME MONITORING

OF VIABLE AND NON-VIABLE PARTICLES.

Polymer pressure regulatorRotork Fairchild has introduced a polymer pressure regulator with features for high-precision pressure control

duties. The Model 55 pressure regulator features a non-rising stem within a small footprint design, delivering

high-precision control in a lightweight and compact package.

Manufactured entirely from polymer and stainless steel, the product offers compatibility with medical and

scientific gases such as nitrogen, helium and oxygen. Applications include precision pressure decay and

leak testing, medical ventilators, respiratory diagnostic systems, balloon pumps and sterilisation equipment.

The venturi design compensates for downstream pressure droop under flow and provides highly accurate

pressure output control under all conditions. The separate control chamber isolates the control diaphragm

from the main flow to eliminate hunting and buzzing.

With a supply capacity up to 150 psi (10 Bar) and flow capacity up to 10 SCFM (283 L/min), the device

controls output pressures up to 100 psi (0.7 Bar) to within ±0.2 psi (14 mBar) or better, even with fluctuating

or rapidly decreasing supply pressure. This high pressure capability, combined with pinpoint accuracy at low

control pressures of 7.5 psi (0.5 Bar) or less, sets the product apart from conventional regulators for demanding

applications.

Rotork Australia

www.rotork.com

history of the sample is available. All the data can be stored securely. Note that the BMS and EMS software must be 21CFR11 compliant after validation.

There are some software packages on the market that combine the EMS and the biological monitoring onto one complete package allowing for real-time monitoring of viable and non-viable particles.

The advantages of using an automated system for particle monitoring outweighs any manual labour systems, which are prone to error and slower. This type of system keeps monitoring operators outside Grade A/B environments and, as previously mentioned, is located off the main cleanroom, thus reducing particle contamination levels and significantly reducing labour costs. Process data can be easily retrieved and reviewed by the quality manager. These systems have become cost effective compared to purchasing portable units.

The portable particle counter is usually used for spot checks or monitoring back-ground counts in lower-grade cleanrooms.

Portable air samplers can be used in the same manner.

The following are some of the advantages when monitoring with EMS and biological monitoring systems.

• Collection of data: The amount of data collected by FMS and BMS is vast compared to portable units. Trending of this data is achieved easily using the software applications. Events can be seen in advance, allowing for corrective action before they effect production.

• Storage of data: The FMS/BMS store all sample data on a dedicated secure server. The software can be validated to comply to Code of Federal Regu-lations section 21 part 11 (21CFR11). Data is easily and securely reviewed.

• Labour: Using a FMS/BMS saves labour costs as the systems are automated and can generally be run and supervised by one operator, significantly reducing labour costs.

• Reliability: EMS/BMS by nature of

their design are more reliable and, in the case of breakdown, plug-and-play can be utilised with spare calibrated units available allowing production to continue as scheduled.

• Alertness: Alarm limits can be pro-grammed into the software. This gives the operator time to react and, with the right action, can prevent contamination before it has an impact on production.

• Cost: FMS/BMS has become a more cost-effective and efficient way to continuously monitor. Labour costs are also significantly reduced.

All licensed drug manufacturers must comply with current Good Manufactur-ing Practice (cGMPS) regulation. Cell and tissue, radio-pharmacy and cytotoxic manufacturers also fall under the same scrutiny, even some research facilities. Compliance must be demonstrated at every stage before a drug or human medicinal product can be released to market.

LAF Technologies Pty Ltd www.biocabinets.com.au

www.eppendorf.com/cccEppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany. All rights reserved, including graphics and images. Copyright © 2014 by Eppendorf AG.

> Unsurpassed quality, clarity, purity and sterility, providing reliable cell culture conditions

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The all new product line of Eppendorf Cell Culture Consumables will truly delight your cells. Its outstanding design, reliability and purity is based on more than 50 years of experience. Products created by experts, developed for perfectionists. Impress yourself!

The new Eppendorf Cell Culture Consumables

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CA

SE

STU

DY NHS Wales rolls out national LIMS program

NHS Wales has completed the first phase of the national rollout of InterSystems TrakCare Lab, a comprehensive, multidisciplinary laboratory information management system (LIMS). The product is designed to support modern laboratories that provide consolidated, comprehensive pathology services.

NHS Wales chose TrakCare Lab, part of the TrakCare unified healthcare information system from InterSystems, as the foundation for its national pathology program to connect 18 laboratories in six health boards across Wales. The program will replace eight different laboratory systems with one TrakCare Lab system, enabling more than 21 million tests per year to be managed by one system. It is an integral part of the One Wales initiative to provide a fully integrated healthcare record across the whole of Wales.

The first phase of the implementation covered all four labs in the Hywel Dda Health Board, each of which ran smoothly with no downtime. Withybush General Hospital was the first to go live on 9 September 2013, followed by Glangwili General Hospital and Prince Philip Hospital on 14 October 2013. The Bronglais General Hospital laboratory, which went live on 3 December 2013, completed the blood sciences and microbiology rollouts.

The LIMS deployment is a collaboration among the Pathology Service, the Pathology Modernisation Agenda for Wales and the NHS Wales Informatics Service (NWIS). The standardisation of pathology test sets will enable consistent diagnostic services across Wales and will reduce the number of unnecessary duplicate or repeat tests, saving time and money. When fully implemented and combined with other national developments in Wales, the LIMS will support key government initiatives to deliver high-quality services to patients within the principality.

The implementation of TrakCare Lab provides a number of benefits:• Better services for patients by providing a single pathology record accessible anywhere the information is needed, reducing the

number of diagnostic tests required and improving safety.• Increased efficiency and effectiveness of care for healthcare professionals by enabling access to a common, up-to-date diagnostic

record for more effective treatment decisions.• Improved pathology services through a reduction in duplicate and repeat testing, as well as the increased ability to share workload

across the service.• Ease of user adoption as a result of an implementation methodology that ensured the more robust system could be deployed with

minimal impact on normal laboratory activities.Dr Mike Simmons, Public Health Wales microbiology consultant for Hywel Dda and one of the first to use the new system, commented,

“We are already seeing many benefits in Hywel Dda, including improved clinical outputs from the microbiology labs, which are having a very positive benefit in respect to patient care.”

Paul Grabscheid, vice president of strategic planning at InterSystems, said the company is “very familiar with the requirements and intricacies of a national laboratory program of this kind”.

“In South Africa, for example, TrakCare Lab underpins the national laboratory service for the management of pandemics and chronic illnesses. This supports approximately 38 million uninsured people through more than 250 laboratories nationwide - an experience we can draw upon for Wales. With the successful Hywel Dda deployment, our focus is to ensure that the remaining health boards go live with equal success.”

The Welsh LIMS national laboratory pathology modernisation program is planned for completion during 2014. The LIMS will also service national specialist services including Cervical Cytology, Welsh Blood Service, Public Health Wales for Microbiology

and Infection Control Services.

Intersystems Corporation (Australia) www.intersystems.com.au

Total milk detection kitThe Romer AgraStrip for Total Milk is now available. Previously, to test for the presence

of milk allergens, one had to decide whether to look for casein or Beta-lactoglobulin as

an indicator for allergenic residues. With the AgraStrip Total Milk, the user can test for

both at the same time.

The product is based on lateral flow technology, which means that results are

available in around 11 min without the need for any special equipment, and does

not require a laboratory. Results are read visually, and the limit of detection is

1 ppm of milk protein.

The test is suitable for testing food contact surfaces, raw materials and finished products.

Australasian Medical & Scientific Ltd

www.amsl.com.au

Swagelok® Pressure Regulators are now an even better choice for all your pressure regulator needs. Why? Well, alongside our proven experience and expertise, our range now covers sizes from 1/8 to 4 in. and all your regulator needs – high-fl ow capability, two-stage, back-pressure and vaporizing models. With our regulators you get accuracy, sensitivity and pressure stability. In short – total predictability. Exactly what you would expect. Visit swagelok.com/pressure.

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Digital microscope for quality controlSmartzoom 5 is a digital microscope from Zeiss,

suitable for quality control and quality assurance

applications in virtually every field of industry. The

integrated complete system comprises an optical

engine (which consists of a zoom, camera and

illumination), a stand with sample stage, objective

lenses, the operating unit and software.

Quick and easy to set up, fully automated, and

equipped with dedicated quality assurance and

quality control components, the product is simple

to operate, even for the untrained user. It features

a macro recording mode to enhance the workflow

for repeat sample analyses of the same type in a

step-by-step manner.

The device is suitable for conducting failure analysis and routine inspections. Once the quality inspector has

placed the sample under the microscope, they no longer have to switch back and forth between the device and

the software; instead, they can carry out all the necessary steps right on the screen. They can also save this

workflow so it is available for use at a later time, making analysis reproducible and independent of the user.

The Best Image feature allows users without any prior knowledge to achieve optimal results; the system

shows a preview of different illumination options, and the user only needs to select the best one. The device

is calibrated at the factory and can be used immediately without requiring any further adjustment.

Carl Zeiss Pty Ltd

www.zeiss.com.au

Ultralow-temperature freezersLabGear Australia has released the Snijder Labs

range of ultralow-temperature freezers. The EvoSafe

series freezers ensure high protection for both the

samples and the environment.

The freezers are fitted with PU insulation between

the interior and exterior panels. The system ensures

all the cooling is transferred to the interior of the

freezer and is said to provide higher insulation val-

ues than traditional foamed in place insulation and

vacuum insulation panel technologies. The freezer

loses less temperature and it takes 12 h to reach -10

from -86°C, compared to 9 h for traditional brands.

The product is said to use 30% less power than

traditional freezers, resulting in less greenhouse gas

emissions. A water-cooled condenser option is also

available, providing even lower power consumption

and lower air-conditioning costs for the laboratory.

The range is available in both upright and chest

versions, with sizes from 120 to 720 L and comes

with data logging, alarm functionality and internet

connectivity.

LabGear Australia

www.labgearaustralia.com

Microplate washerMultiWash+ is an automated microplate washer that provides maximum

flexibility with a minimal footprint. The MultiWash+ Microplate Washer

can be configured for both 96- and 384-well plates and comes with four

wash/rinse bottles and one waste bottle for out-of-the-box use.

The onboard touch panel comes with the option of 20 different wash

protocols, including up to eight cycles within each protocol for walk-up

usability and flexibility. Washing variations include adjustable speed and

volume; adjustable aspiration speed and time; and adjustable soak times.

Three modes of shaking are available to mix solutions.

Efficient washing is achieved with cross-wise aspiration-reducing

residual volume within each well. The washer has a small footprint and

is vacuum- and pressure-free, with onboard pumps that provide a quiet

wash experience.

Bio-Strategy Pty Ltd

www.bio-strategy.com

YOUTILITY – TOUCH THE FUTURETHE DURAN® LABORATORY BOTTLE SYSTEM

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By choosing the right pipetting system and eliminating risk and variability from pipetting procedures, users can achieve enhanced accuracy, reduced costs and better overall productivity.

Evaluating application workflow is critical to selecting a pipette that will achieve accuracy. This paper explores helpful pipetting recommendations that can help users make educated choices in liquid handling instruments for

greater ease of use, accuracy and instrument life, while reducing error risk and costs associated with failed experiments, rework and even repetitive stress injuries (RSIs).

Correctly assessing and reducing risks throughout a liquid han-dling workflow is critical to pipette selection and ongoing pipetting accuracy. Pipette selection can benefit from a full planning process which analyses all workflow aspects to determine liquid handling requirements for maximum efficiency and accurate data genera-tion. These workflow aspects determine the pipette type required to achieve accurate results and enhance productivity.

Perhaps the most critical aspect to consider for any pipetting system is maximum tolerated data variability. This ensures a cho-sen apparatus can deliver required accuracy. Knowledge of sample type, liquid-handling volume/throughput and sample/assay specificity is also required to select handling formats that will provide the greatest accuracy and productivity for the process.

Nature of the sampleMost pipetting is straightforward. Aqueous samples at standard lab temperature and pressure can be pipetted accurately using simple air displacement. However, difficult samples such as viscous, dense, volatile, hot or cold liquids may be pipetted more accurately using

positive displacement, which uses a piston and seal instead of an elastic air cushion to move liquid.

Helpful positive-displacement pipette attributes include less user training required for accuracy. Disadvantages include higher costs for disposable capillaries and pistons, and greater ergonomic forces required to plunge when pipetting manually. Fewer multichannel options are available as well, which can slow processes at higher throughput volumes.

VariabilityBecause some processes are more sensitive than others and some variability is expected, users must identify how much variability is tolerable to still produce solid data based on an individual process. Experiments dependent on a standard curve or generated through serial standards dilution can be severely affected by any amount of sub-optimal pipetting no matter how small, making features and expected accuracy of pipettes under consideration particularly critical to these efforts.

Pipetting volumeWorkflows that require a large amount of liquid handling early, such as preparing buffers or plating cells, must often be balanced against smaller volume handling requirements later in the process. Needs for speed and precision must be carefully considered to manage both ends of this processing spectrum. Consistently working at the outer limits on either the high or low end of a pipette’s volume-

How application and workflow affect pipette selectionNoah Saederup, PhD, Product Manager


handling capability can compromise accuracy. If the number of samples being processed is high enough, significant efficiencies and even cost savings over time may be gained by moving assays to a plate format and shifting to multichannel pipettes.

Multichannel pipettes are an important option for fast, high-throughput applications such as 96-well plate ELISA work, or PCR and qPCR. Good multichannels such as 8- and 12-channel models should provide fast, secure, simultaneous tip-loading. They should not require excessive force to mount tips evenly, and tips should eject easily. A full pipetting platform, such as a manual 96-well pipettor, may also be helpful.

Adjustable spacer multichannel pipettes can also be useful for transferring multiple samples between different-sized tubes and plates with ease. This can cut format change time by up to 85% because as many as eight samples can be moved at the same time.

Manual or electronic?Complex or repeated pipetting may benefit from electronic pipettes, since electronic pipettes can be programmed for specific protocols. They have also been shown to produce more consistent data than manual pipettes because microprocessors eliminate human error and variability - particularly noticeable in applications where pipetting errors can be compounded such as serial dilution.

Furthermore, electronic pipettes tend to be more ergonomic than manual pipettes. Ergonomic enhancement is usually achieved because the electronic mechanism reduces operator force required

to plunge. It also reduces repetitive motion through the operator’s ability to program activities such as multidispensing, automatic mixing and serial dilution.

Cost may be a concern with electronic pipettes, however. Manual pipettes tend to be more economical, though they produce greater variability. However, particularly for highly sensitive or expensive pipetting applications, the cost of investing in an electronic pipette may be offset by a reduction in error and rework in a short span of time.

Choosing the right tipThe design, quality and fit of the pipette should be considered when choosing the right tip.

Design refers to properties that affect performance in different applications. For example, a wide orifice tip will behave differently from a low retention one. Standard tip design for regular, all-purpose pipetting includes thin clear walls to see the liquid being dispensed, a fine point for accurate dispensing and a good seal. Many specialty application tips also exist.

Quality refers to how a tip is manufactured, including whether it is free of defects that can cause costly sample loss and contami-nants that disrupt experiments. This is a critical concern because some polypropylene labware consumables have been found to leach plasticisers which interfere with certain enzymatic reactions or cause false 260/280 nm spectrometer readings.

Fit refers to how well the tip matches the pipette. In almost every case, it is best to use a manufacturer’s recommended tip to gain the best fit, considering the tip-pipette a ‘comprehensive system’ rather than trying to use another brand of standard or universal tips.

Application-specific requirements should also be considered. For example, genomics application best practices include use of filter tips to minimise the effect of human or microbial DNA, RNase, DNase, Pyrogen or ATP on the sample or pipette. Filters can also block aerosols from the shaft, reducing contamination of later samples as well. They help protect the piston from microbial contamination, corrosives and salt deposits as well.

ConclusionPipetting is a critical action in many labs, and data accuracy/reproducibility generated by a liquid-handling workflow can be significantly improved by understanding application aspects such as the nature of the sample, volume, throughput and workflow-related risks. Tolerated variability is also a critical consideration. Selection of the right liquid handling instruments and options flows naturally from this analysis.

Taking time to analyse pipetting workflows can benefit users by providing greater processing ease and enhancing productivity.

Mettler Toledo www.mt.com/rainin

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Stable cell line development servicesGeneCopoeia offers services for establishing

stable cell lines for protein overexpression,

gene knockdown or genome editing which

meet the user’s specific research needs.

Stable cell lines produced by the company

can overexpress virtually any protein of inter-

est in the user’s cell line of choice. Users

can choose to have their own expression

cassette integrated into the genome or from

among more than 53,000 human and 32,000

mouse ORFs from the ORF clone collection.

Applications include monoclonal antibody

production; protein production for biochemical

assays; protein production for crystal or NMR

structural determination; fusion tagging for

live cell imaging; fusion tagging for pulldown/

immunoprecipitation; drug target analysis.

GeneCopoeia will design shRNAs and

integrate them into virtually any cell line.

Applications include determination of gene

function in cells; expression profiling to identify

candidate downstream genes in a network or

pathway; drug target validation.

The company also offers stable cell lines

carrying specific, targeted modifications cre-

ated with either TALEN or CRISPR-Cas9. They

will design the genome editing tool target

site, donor constructs used for homologous

recombination-mediated applications, transfect

the cell line of choice with the genome edit-

ing tools and isolate single or double allele-

modified clones. The service can also be

used with the human and mouse safe-harbour

integration system or human safe-harbour

knock-in ORF clones.

Applications include gene knockout; intro-

duction of point mutations or defined insertions/

deletions; correction of disease mutations

back to wild type; transgene knock-in at a

customer-specified locus or at human and

mouse safe-harbour sites; gene tagging;

promoter or gene replacement.

United Bioresearch Products

www.unitedbioresearch.com.au

CentrifugesThe Avanti JXN-26 centrifuges from Beckman Coulter

Life Sciences offer laboratories a large intuitive touch-

screen user interface and data management features to

free user time in research and bioproduction. Scientists

can track and manage centrifuge runs remotely by

logging in to the MobileFuge app from an Apple iOS

or Android device. Researchers and facility managers

can leverage data management, user tracking, net-

working and remote capabilities for ease of use and

efficiency in multiuser biological sample preparation

environments, and to meet quality control standards.

The product provides high performance and application

versatility with wide rotor compatibility and capacity up to 6 L,

speeds to 26,000 rpm and relative centrifugal forces up to 81,700 x g. The

rotor library comprises 26 options including fixed-angle and swinging-bucket models

with lightweight designs that accommodate a variety of tubes, microplates and bottles.

BioCertified rotors, in conjunction with appropriate centrifuges and labware, are available for

biosafety options. The centrifuges also provide energy savings and environmental advantages.

A friction reduction system (FRS) lets the device run more efficiently and quietly by low-

ering the heat present in the chamber, reducing refrigeration system usage and running

costs. A low work surface makes rotor handling easier and a foot pedal on the centrifuge

is used for hands-free lid opening. An automatic detection system prevents the centrifuge

from being run at higher than rated speeds for the installed rotor type.

Beckman Coulter Australia

www.beckmancoulter.com

Sample preparation kitsPrecellys Lysing Kits are robust tubes prefilled with high-quality beads, suitable

for processing biological samples prior to extraction of DNA, RNA, protein,

drug or live cells. By choosing the right Lysing kit for each sample, based

on scientific publications and protocols, it is claimed that users will save time

and improve on current lab practices.

Whatever the sample, be it animal tissue, human

tissue, plant tissue or microorganisms, users can

find a suitable Precellys Lysing kit from the 15+

available kits. Combined with the powerful bead-

beating technology offered by the Precellys range of

homogenisers, the user can dry grind, homogenise

or lyse tissue as hard as bone or as soft as liver.

Choosing the right Lysing kit and protocol

(homogenisation speed and cycle length) is

easy with 70+ application notes, 1000+ pro-

tocols and 2000+ publications available. It is

said that by choosing the right tube size (0.5,

2 or 7 mL), bead type/diameter (ceramic, glass or stainless steel, from 0.1 to

6.8 mm), users will improve on their current sample preparation method.

Users will obtain good DNA, RNA, protein, drug or live cells recovery, save

time and get consistent cross contamination-free preps. The kits are certified

DNase/RNase-free.

Sapphire Bioscience

www.sapphirebioscience.com

http://www.lonza.com/4d-nucleofector


CA

SE

STU

DY Heating block systems support synthesis research

Monash University’s School of Chemistry has invested in a range of Asynt DrySyn Multi heating block systems to support its groundbreaking research in photovoltaics, water splitting and nanoimaging.

Photovoltaic research being undertaken by Professor Leone Spiccia and his group is mainly focused on finding new electrolytes and redox couples for dye-sensitised solar cells and optimising other parameters for the cell assembly. The water-splitting team is working on artificial photosynthesis, a concept to convert solar energy into a storable form of energy by splitting water into hydrogen and oxygen. The nanoimaging team is pursuing multidisciplinary projects to design, prepare and characterise functionalised nanomaterials intended for application including multimodal imaging, as well as therapeutic and diagnostic agents for early detection and treatment of cancer.

Professor Spiccia explained, “As part of this research we synthesise nanoparticles based on lanthanides, for which we must reach high temperatures (>300°C). “For this work, we need to have precise fine control of both the final temperature and the heating speed together with a good heat transfer to obtain good-quality particles with a narrow size distribution,” Professor Spiccia continued. Oil bath systems are traditionally used for all general syntheses, but according to Professor Spiccia, “We could not use an oil bath because of the high temperatures and heating mantles cannot provide the control we needed for the synthesis.

“After a lengthy evaluation process, we decided to switch to DrySyn heating block systems as they are inherently safer to use than heated oil baths, avoiding the risk of oil spillage that can lead to burns when hot or may cause someone to slip over.” The Asynt DrySyn Multi provides a safe and convenient way to perform precisely controlled heated reactions in parallel. It converts any standard hotplate stirrer into a reaction block accommodating three flasks or up to 12 reactions in tubes or vials. Made of chemically resistant, anodised aluminium, the heating blocks offer good heating

performance to over 300°C and can heat a reaction flask 25% faster than an oil bath. “DrySyn has been really important to us to achieve good research results,” Professor Spiccia concluded.

Asynt http://www.asynt.com/

MALS detector and RI detectorWyatt Technology has announced an application note

which outlines the combined use of the Wyatt

µDAWN multiangle light scattering (MALS)

detector and the Optilab UT-rEX refractive

index (RI) detector to measure the absolute

molecular weight (or molar mass) and size of

eluting species in ultrahigh-performance liquid

chromatography (UHPLC). The instruments,

engineered for use with ultrahigh-performance

size exclusion chromatography (UHP-SEC), are said to

overcome the limitations associated with conventional UHP-SEC

for the characterisation of size and composition of aggregates or fragments in a biomolecular sample.

Conventional SEC relies on reference standards to calibrate column elution time as a function of

molar mass. However, these standards often do not accurately represent the conformation of the

sample, or the sample may exhibit column interactions that change its elution properties relative to the

standard. The application note discusses how UHP-SEC-MALS provides improved resolution, higher

throughput, less solvent and smaller sample consumption for analysis of precious biological samples

in comparison to standard SEC-MALS. Light scattering detectors designed for standard HPLC have

generally been incompatible with UHPLC, but the combination of the µDAWN and UT-rEX results in fast

and efficient characterisation of biomolecular samples. The detectors are said to maintain the narrow

peak widths typical of UHPLC while providing even more information than standard SEC-MALS due

to the upstream resolution obtained with 300 mm UHPLC columns. The time difference between the

two techniques demonstrates how UHP-SEC-MALS results in faster analyses.

With the introduction of the µDAWN, users have access to all the in-depth analyses available with the

miniDAWN TREOS for UHPLC. The range of instrumentation, providing triple detection with UV, MALS and

RI, also enables the application of protein conjugate analysis to identify the amount of post-translational

modification, associated detergent, drug-conjugate or other additions to a polypeptide background.

Shimadzu Scientific Instruments (Oceania) Pty Ltd

www.shimadzu.com.au

Professor Leone Spiccia.

DrySyn Multi on a stirring hotplate.

LIMSTo enable laboratories to be more flex-

ible, efficient and compliant, software

should empower users and improve

productivity across a connected

enterprise. SampleManager 11 puts

decision-making power in the hands of

users who can make logical choices

about workflow, instrument integration

and data reporting for management

metrics or regulatory requirements.

Today’s labs have the potential

to be paperless, powerful enterprise

drivers for business transformation.

A workflow-driven LIMS delivers the

agility necessary for businesses to

transform as the regulatory or eco-

nomic environment requires.

Thermo Fisher Scientific

www.thermofisher.com.au

Crowning achievement - System solutions for analytical grade laboratory glassware reprocessing

Benefit from the largest range of baskets and inserts!Washers and disinfectors by Miele Professional for machine-based reprocessing:• analytical grade• gentle on materials• ecologically soundBecause high quality laboratory equipment deserves a professional clean.

Call: 1300 464 353www.miele-professional.com.au

http://www.miele-professional.com.au


Water testersThe Pocket Pro and Pocket Pro+

testers measure electrochemical

parameters in a broad range of water

applications. The family of 12 testers offers

convenient portable solutions for pH, ORP,

conductivity, TDS, salinity and temperature,

delivering accurate results.

The product runs on AAA batteries and includes

a convenient sample cup to ensure maintenance and

measurements are simple. It also features a large LCD

screen, easy calibration steps and built-in diagnostics

for pH testers.

The Pocket Pro+ testers include replaceable sensors,

a powerful backlight and multiparameter tester options.

Hach Company

www.hachpacific.com.au

Torque testerThe Mecmesin Helixa Torque Tester is suitable for measuring low and medium torque variations with

high accuracy and control on a variety of delicate or finely engineered products. Its sensitivity and

precise alignment make it useful for testing high-quality assemblies such as those found in the cos-

metics, medical, pharmaceutical and jewellery industries.

The product is designed for precision design applications where torque values may be very small

and where accuracy is paramount. Its counterbalancing mechanism allows the removal of axial force

while also enabling the application of precise top-load or tensile force when required. The drive spindle

can be user aligned for precise concentricity with the intelligent torque cell.

The device accepts interchangeable intelligent torque cells for measure-

ment from just a few Nmm up to 6 Nm, with a resolution of 1:6500 and

an accuracy of ±0.5% of full scale. Controlled by the company’s Emperor

software, the Helixa-i is connected to a PC or is also available as a touch-

screen model, the Helixa-xt, with all the same features in the convenience

of a self-contained unit.

SI Instruments

www.si-instruments.com

Automated cell separation kitDiaSorin has launched Liaison Ixt/Arrow CellSep Ad-

vanced for the automated isolation of cells directly from

whole blood or buffy coat. Based on magnetic bead

technology, the product can isolate up to three cell types

per sample in just 32 min per cell type, offering time

savings compared to manual cell separation methods.

The device is designed for use on the Liaison Ixt/Arrow

instrument for the separation of one, two or three cell

types in up to 12 samples at a time. Requiring mini-

mal hands-on intervention, it produces good yields of

purified cell preparations ready for use in a wide range

of downstream applications, including lineage-specific

chimerism analysis. The automated platform also mini-

mises human error and ensures reproducibility between

runs for consistent and reliable downstream analysis.

The instrument can also be used for DNA extraction

using the Ixt/Arrow Blood DNA extraction kit, ensuring

streamlined processing of samples and saving bench

space in the laboratory.

DiaSorin

www.diasorin.com

Transcriptomic analysis kitsThe REPLI-g WTA Single Cell Kit and REPLI-g Cell WGA &

WTA Kit enable transcriptomic NGS analysis from single cells

and comparative genomic and transcriptomic analysis from the

same small sample.

Both kits expand the REPLI-g single cell kit portfolio, which

can make single cells accessible to NGS analysis by amplifying

either DNA or RNA from individual cells.

Using Qiagen’s MDA with the SensiPhi polymerase (said to

have 1000-fold higher accuracy than Taq polymerase), the ampli-

fied nucleic acids show comparable performance to the original

molecules, which allows users to perform multiple experiments

from a single cell reliably and reproducibly.

Qiagen Pty Ltd

www.qiagen.com


Researchers from Harvard University’s Wyss Institute for Biologically Inspired Engineering have created a new organ-on-a-chip - a microfluidic device that mimics the physiology of a living organism. The device reproduces the structure, functions and cellular make-up of bone marrow.

Bone marrow is an incredibly complex organ that is respon-sible for producing all of the blood cell types of our body, and our bone marrow chips are able to recapitulate this complexity in its entirety and maintain it in a functional

form in vitro,” said Dr Don Ingber, founding director of the Wyss Institute and senior author of the study.

Writing in the journal Nature Methods, the researchers stated, “Current in vitro hematopoiesis models fail to demonstrate the cel-lular diversity and complex functions of living bone marrow; hence, most translational studies relevant to the hematologic system are conducted in live animals.” But as co-author Dr Yu-suke Torisawa noted, human and animal responses are totally different. The team thus set out to engineer artificial bone with living marrow.

The task would be a complex one, as bone marrow has an integral relationship with bone. Marrow sits inside trabecular bone - a solid-looking type of bone with a porous, honeycombed interior. Inside, conditions vary between areas, and the dozen or so cell types each have their own preferred spots. Furthermore, bone marrow cells communicate with each other by secreting and sensing a variety of biomolecules, which act locally to tell them whether to live, die, specialise or multiply.

“In order for bone marrow to produce all of its functions, all these little areas and niches serve a role,” said co-author Catherine S Spina. “Certain cells line certain parts of the bone marrow, so if you don’t have the proper bone architecture, you will not have the proper physiology.”

“Most organs on chips, we simplify down to the most basic ele-ments and we build back,” explained Dr Ingber. “Here, we have an incredibly complex system and we maintain the complexity in vitro.”

Rather than trying to reproduce such a complex structure cell by cell, the researchers enlisted mice to do it. Spina and Dr Torisawa packed dried bone powder into an open, ring-shaped mould the size of a coin battery and implanted the mould under the skin on a mouse’s back. After eight weeks, they surgically removed the disc-shaped bone that had formed in the mould. It revealed a honeycomb-like structure that looked identical to natural trabecular bone. When the researchers stained the tissue and examined it under a microscope,

the marrow was packed with blood cells, just like marrow from a living mouse. And when they sorted the bone marrow cells by type and tallied their numbers, the mix of different types of blood and immune cells in the engineered bone marrow was identical to that in a mouse thighbone.

To sustain the engineered bone marrow outside of a living animal, the researchers placed the engineered bone in a microfluidic device that steadily supplied nutrients and removed waste to mimic the circulation the tissue would experience in the body. The finished product, bone marrow-on-a-chip, gives scientists a much-needed new tool to test the effects of new drugs and toxic agents on whole bone marrow, remaining healthy for up to one week.

The device could be used to develop safe and effective strate-gies to prevent or treat radiation’s lethal effects on bone marrow without resorting to animal testing. In an initial test, the engineered bone marrow, like human marrow, withered in response to radiation unless a drug known to prevent radiation poisoning was present.

Bone marrow-on-a-chip could also generate blood cells, which could circulate in an artificial circulatory system to supply a network of other organs-on-chips. The Defense Agency Advanced Research Projects Agency (DARPA) is currently providing funds for the Wyss Institute to develop such a system - an interconnected network of 10 organs on chips to study complex human physiology outside the body.

“You can imagine a system in which we connect individual organs, including the heart and the lungs, and we use the bone marrow-on-a-chip to produce the blood that circulates through all the organs, thus connecting the system and really recreating human physiology,” said Spina.

Other potential uses include maintaining a cancer patient’s own marrow temporarily while they undergo marrow-damaging treatments such as radiation therapy or chemotherapy; and growing human bone marrow in immune-deficient mice.

Image: Microscopic view of the engineered bone with an opening exposing the internal trabecular bony network, overlaid with coloured images of blood cells and a supportive vascular network that fill the open spaces in the bone marrow-on-a-chip. Credit: James Weaver, Harvard’s Wyss Institute.

“

Building bone marrow-on-a-chip

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Nanoparticle tracking analysis and dynamic light scattering

Exosome research can be constrained by a

lack of suitable methods for characterisation

as they fall below the detection range of

many currently used techniques. Existing

techniques such as electron microscopy

offer high-resolution images; however, they

usually require time-consuming preparation

and analysis, which could potentially dam-

age the sample.

Nanoparticle tracking analysis (NTA) coupled

with dynamic light scattering (DLS) offers an approach

that is fast, easy to use and accurate. The Malvern NanoSight with NTA allows specific

bionanoparticles in liquid suspension to be directly and individually visualised and

counted in real time. Particle-size distribution profiles in the range of 10-2000 nm

(sample dependent) and concentration measurements are determined simultaneously.

Operation in fluorescence mode enables characterisation and differentiation of suitably

labelled particles using a range of excitation wavelengths.

The Malvern Zetasizer series uses DLS to extend the measurement range to below

1 nm. The product also enables the measurement of zeta potential, a measure of

the magnitude of repulsion or attraction between particles which affects stability and

interactions. By combining size distribution, zeta potential DLS measurements and

the capabilities of NTA, scientists are able to get a more complete picture of nano

processes taking place, including aggregation.

ATA Scientific Pty Ltd

www.atascientific.com.au

Assayed controlsThe TGA has cleared two Randox liquid assayed chemistry controls for use in Austral-

ian medical laboratories. Randox believes the controls will revolutionise quality control in

laboratories, saving time and money while ensuring accurate results and patient diagnosis.

The controls, Acusera Liquid Assayed Chemistry Premium and Premium Plus, are de-

signed to cut the number of individual quality controls (QC) a laboratory needs to run in

routine medical testing. Laboratories can run QC up to 100 analytes all within one control.

The assayed controls contain a combi-

nation of proteins, lipids, immunoassays,

cardiac markers, blood markers, therapeutic

drugs and routine chemistry analytes. The

Acusera Chemistry Premium Plus control

contains 100 analytes, while the Acusera

Chemistry Premium control offers 91. The

high levels of CRP and other key proteins

included eliminate the need for multiple controls, streamlining QC in laboratories. The

assayed controls provide clinically significant values for a wide range of analysers at key

decision points. Both controls are liquid for ease of use, eliminating possible reconstitution

errors and improving lab efficiency. They are highly stable when stored at -20 to -70°C

with open vial stability of up to seven days at 2 to 8°C, reducing wastage in laboratories.

The controls are manufactured using human-based serum, ensuring performance that

closely mirrors patient samples and assuring laboratories of unbiased, independent as-

sessment of analytical performance. Available in three concentration levels (low, mid and

high), Premium Plus covers the full clinical range, removing the need for laboratories to

purchase additional low- or high-level controls.

Randox (Australia) Pty Ltd

www.randox.com

Tissue sample marking softwareTissueMark software developed by PathXL

provides pathologists with the potential to

measure hundreds of cancer characteristics

in tissue. The product is said to improve the

way laboratories approach the process of de-

termining the percentage of tumour contained

within any given sample.

It is difficult, even to the highly skilled

professional, to make precise estimations.

TissueMark analyses the detailed structural

patterns in tissue samples and marks the

boundaries of potentially cancerous sections

for more detailed analysis. As it combines the

benefits of digital workflow management with

fast, accurate and consistent estimations, the

company’s algorithms allow the software to

analyse the same amount it would usually

take an experienced pathologist one day to

do - in only 10 min and with precision.

Pathologists can instantly look at the result

of the automated analysis and make their own

evaluation of it, having the power to redraw

the tissue’s tumorous boundaries if they wish

so, make annotations and digitally send results

to colleagues for immediate consultation. The

product thus empowers the experts to put

their efforts into their most important work,

such as the accurate and timely diagnosis

of tumours and other disorders.

The software allows for increased quality

and standardisation of tumour cell measure-

ment and macrodissection. As the number of

inaccurate estimates decrease, this mitigates

the risks for patients, who can benefit from an

in-depth, detailed knowledge of their condi-

tion by their healthcare providers as well as

targeted therapy to combat it.

The product currently covers three of the

most common forms of cancer - lung, colo-

rectal and breast. Further modules will be

released soon.

OnQ Software Pty Ltd

www.onqsoft.com.au


CARRYMOREWATERMETHODSWITHYOUWHEREVERYOUNEEDTHEMMOST.

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The DR 1900 PortableSpectrophotometer featuresover 220 of the most commonlytested water methods.

270x88mm HACH PACIFIC_LabLife_DR 1900:layout 5/16/14 8:35 AM Page 1

QuEChERS kitsPhenomenex has introduced roQ QuEChERS kits for the cleanup of

multiple pesticide residues in food prior to HPLC or GC analysis.

The kits offer a number of features specifically designed

to facilitate and improve the largely manual QuEChERS tech-

nique. Rigorously tested tubes and tube caps ensure leak-free

performance. The low-extractable tubes ensure clean extracts.

In addition, roQ flat-bottom centrifuge tubes are designed to

stand upright so they can be easily placed on a scale for sam-

ple weighing. The roQ salt packets have an easy-pour design

that minimises spills.

QuEChERS (quick, easy, cheap, effective, rugged and safe)

is a two-step sample preparation approach for the extraction

and analysis of multiple residues, which are difficult to target

with selective techniques such as SPE. The technique extracts

a wide range of compounds of various chemical structures and

removes unwanted interferences in the sample. Phenomenex’s

roQ is compatible with official methods from AOAC International

and the Committee of European Normalisation (CEN).

A wide range of roQ QuEChERS extraction and dispersive

SPE (dSPE) kits is available to meet the needs of different food

matrices. Phenomenex offers a range of solutions for subsequent

high-resolution analysis of multiresidues by LC or GC.

Phenomenex Australia

www.phenomenex.com

Automated liquid-handling platformThe Gilson Pipetmax is

said to reduce technical

replicates and maximise

reproducibility. Bringing

a multitude of applications to

the user’s disposal, such as qPCR,

NGS, cell-based assays and more, the product is claimed to increase

the reproducibility and pace of experiments.

Manual sample preparation can often be inefficient, complex and

time consuming, leading to preparation time, procedural errors and

operational costs. Pipetmax is an easy-to-use, automated liquid-

handling platform than can solve these problems with automated

sample preparation solutions. The unit is accessible to any lab and

has a pipetman inside.

The product provides high levels of precision and accuracy. Pro-

cedural errors become a thing of the past, increasing the lab’s pace.

The platform-independent solution works with a variety of different

reagents and kits, and the unit fits on the user’s lab bench. Trace-

ability is maximised with simple, automated run reports.

John Morris Scientific Pty Ltd

www.johnmorris.com.au

http://www.hachpacific.com.au


Material ID handheld Raman analyserThe Rigaku Progeny is a handheld analyser designed to

deliver comprehensive and accurate materials identifica-

tion. The analyser has been designed for pharmaceutical,

chemical manufacturing and academia markets.

The product’s miniature VPG-based 1064 nm optical

engine offers benchtop-quality analytical performance in

a rugged, ergonomic and IP68 sealed enclosure. The fully

customisable workflow software is 21 CFR Part 11 compliant

and has a smartphone-inspired user interface, allowing rapid

implementation of material ID methods.

The 1064 nm excitation laser optimises the speed and sensitivity of

analysis, minimises fluorescence interferences and broadens the range of measurable materials.

The 512-pixel InGaAs detector delivers good resolution, analytics and signal to noise, handling

challenging mixture analyses. The ultrafast quad-core processor manages demanding search and

quantification algorithms, while the sampling accessories and adjustable focus optimises sensitivity.

The product’s sleek shape provides easy, single-hand entry into industrial barrels/drums, al-

lowing users to view the screen while the instrument is in a downward orientation. The sealed

system guards against moisture and dust, enabling rugged warehouse use and easy cleaning

while minimising cross-contamination risk.

The multitouch, high-contrast screen and large-sized hard buttons make the device easy to

use in labs, warehouses or in the field. Remote connectivity is enabled using both Wi-Fi and

Bluetooth. The integrated digital camera for barcode entry and sample snapshots improves

workflow efficiency and data tracking.

The intuitive user interface allows for simple development of new applications and adaptation

of current workflows. The product runs simple or advanced chemometric applications directly

on board the analyser for both qualitative and quantitative method development. The company

says its wavelets-based spectral match algorithm ensures confidence in sample identification.

Australian X-Ray Tubes Pty Ltd

www.axt.com.au

Workstation for bone decalcificationThe Milestone BoneSTATION is a workstation for fixation and decalci-

fication of bone tissues, providing the user with complete control of

pre-analytical steps. The system is said to improve turnaround times

for bone decalcification and provide accurate diagnostic results for

morphology and molecular studies.

The product consists of two work platforms complete with

glass modules for fixation/decalcification. The front platform, for

the decalcification step, features a heating plate with infrared

sensors for automatic temperature control up to 50°C and

magnetic stirring. The rear platform, for the fixation step, has

built-in magnetic stirring.

The station can be used with any type of fixative/decalcifier, even with strong mineral acids (HCl-

HNO3), as all contact surfaces are either glass or PTFE polymer. The magnetic stirrer assures ho-

mogeneity of temperature throughout the solution. The PTFE cover condenses vapours, generating

a reflux of the reagent for consistent and safe protocols at constant pH.

A touch-screen terminal allows the user to optimise, standardise and fully document all the pro-

cesses. A transparent fume hood is available as an option for operation with strong acids.

Abacus ALS Australia

www.abacus-als.com

http://www.mep.net.au


NT-proBNP ELISA for determination of heart failureNT-proBNP brain natriu-

retic peptide, a member

of the cardiac natriuretic

hormones (CNHs) family,

is secreted by the hemodynamically stressed heart, mainly in response

to myocardial stretch induced by volume overload. The NT-proBNP

ELISA from Biomedica provides reproducible results with an easy-to-use,

convenient 96-well protocol (5 standards, 1 control). The kit has been

designed for manual use or can be adapted to open ELISA systems.

BNP is synthesised as a 108 amino acid residue precursor and,

upon release into circulation, is cleaved into an N-terminal fragment

(NT-proBNP) and the biologically active hormone (BNP-32). NT-proBNP

has a longer half-life in circulation (120 min) compared with the C-

terminal BNP peptide (20 min). Therefore, the measurement of NT-

proBNP offers good stability in serum/plasma, giving reliable results.

NT-proBNP has clinical significance for: detection of left ventricular

dysfunction (LVD); differentiation between chronic obstructive pulmo-

nary disease (COPD) and chronic heart failure (CHF); risk stratification

after myocardial infarction (AMI); therapy monitoring of heart failure

patients. The ELISA is CE marked, allowing for possible IVD use, and

has a wide dynamic range which enables accurate detection of early

stages of heart failure.

United Bioresearch Products

www.unitedbioresearch.com.au

Microplate dispenserBioTek’s MultiFlo FX microplate dispenser is now available with optional

RAD (random access dispense) technology to further extend its application

range and flexibility. The technology offers single-channel dispensing to

random individual microplate wells, along with rapid reagent dispensing

into large-volume wells of 6- to 24-well microplates. The product can

also be separately configured with an optional wash module for 6- to

384-well plates. When incorporating RAD technology or the washing

module, the dispenser replaces up to five separate liquid handlers. The

modular architecture allows users to purchase modules required now and

upgrade as their assay needs evolve, eliminating the need to purchase

multiple instruments.

The device features the company’s Parallel Dispense technology, which

allows up to four independent reagents to be dispensed in parallel without

risk of fluid carryover. A choice of peristaltic or syringe pump dispense

technologies provides reagent conservation and unattended operation

down to 500 nL, with low maintenance and low consumable costs.

The product is especially suited for sensitive cell-based assays, ELISA

and bead-based assays, with angled dispense tips and flexible flow rates

to keep cells intact. When integrated with BioStack 4 Microplate Stacker,

the process can be automated using lidded or non-lidded plates. Fast

programming via intuitive onboard software and a colour touch-screen

display provides total control of dispense patterns, and two integrated

USB ports provide convenient file transfer and storage.

Millennium Science Pty Ltd

www.mscience.com.au

http://www.scitek.com.au


Researchers from Harvard University have accidentally stumbled upon a shape called the hemihelix. While a related shape, the helix, is one of the most common structures in nature, the hemihelix is more complex and rarely seen.

A helix is a 3D spiral structure, much like a corkscrew or a slinky toy. Hemihelices form when the direction or handedness in which the spiral turns - known as the chirality - changes or reverses periodically along

the helix’s length. This reversal in chirality is called a perversion.The team discovered the shape when they set out to fabricate

new springs by taking two strips of rubber material of different lengths and stretching the shorter one to reach the same length as the longer one and then sticking them together.

“We expected that these strips of material would just bend - maybe into a scroll,” said David R Clarke, Extended Tarr Family Professor of Materials at the Harvard School of Engineering and Applied Sciences. “But what we discovered is that when we did that experiment we got a hemihelix and that it has a chirality that changes, constantly alternating from one side to another.”

Writing in the journal PLOS ONE, the researchers said that in its simplest form, a helihelix “consists of two helices of opposite chirality joined by a perversion”, but that “a recent, simple experi-ment using elastomer strips reveals that hemihelices with multiple reversals of chirality can also occur, a richness not anticipated by existing analyses”. To better understand whether the observed 3D structures were randomly occurring or whether specific fac-

Illustration of a helix (top), a hemihelix with one perversion marked by an arrow (middle) and a hemihelix with multiple perversions (bottom). The scale bar is 5 cm for each image. Image courtesy of Jiangshui Huang.

Starting with two long elastomer strips of different lengths, the shorter one is stretched to the same length as the other. While the stretching force, P, is maintained, the two strips are joined side by side. Then, as the force is slowly released, the bi-strip twists and bends to create either a helix or a hemihelix. Image courtesy of Jia Liu.

tors controlled their formation, the scientists stretched, joined and released rubber strips, then numerically simulated and analysed the shape-forming process. Their experiments can be viewed on YouTube.

By testing differences in the aspect ratio, or the width-to-height ratio of the rubber strips, the authors discovered that when a strip is wide relative to its height, it produces a helix. Further measure-ments revealed that there may be a critical value of the aspect ratio at which the shape transitions from a helix to a hemihelix, with periodic reversals of chirality.

The authors suggest that this phenomenon has not yet been observed because other classes of materials simply break when stretched to the mismatched strains used in these tests.

“Once you are able to fabricate these complex shapes and control them, the next step will be to see if they have unusual properties; for example, to look at their effect on the propagation of light,” said co-author Dr Katia Bertoldi, an associate professor at Harvard.

Knowing precisely how to make the structures predictably and consistently may enable scientists to mimic these geometrical features in new molecules that may eventually lead to advances in modern nanodevices, including sensors, resonators and elec-tromagnetic wave absorbers.

Harvard scientists create the hemihelix

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Magnetic stirrersHeidolph offers safe, convenient magnetic hot plate stirrers with a typical

operational life span in excess of 10 years. The stirrers are suitable for

smooth to intense mixing and heating of low-viscosity fluids or decom-

posing organic and inorganic substances.

The magnetic stirrers feature a KeraDisk hot plate, which allows for

efficient heat transfer and fast heat-up times while being highly scratch

and chemical resistant. Compared to lab mixers of 600 W, the MR

Series of hot plates offer a heating capacity of 800 W, which

reduces heat-up times by 35%.

An important safety feature to avoid unintentional heat-up is

the separate illuminated on/off button for heating. Furthermore,

an overheat sensor will switch off the hot plate if overheating

occurs and the unit heater will automatically turn off in case of any

other unforeseen accidents, such as a short circuit or motor failure.

The aluminium die-cast housing is said to make the hot plates virtually

indestructible to thermal damage and also offers high corrosion resistance. The hermetically

sealed housing will ensure that all electronic and mechanical components are protected from

aggressive fumes typically present in fume hoods. Maintenance costs are therefore reduced,

which makes the hot plate mixers a suitable choice, especially for inexperienced users.

The hot plate stirrers are compatible with Heidolph’s wide range of heat-on attachments,

thus increasing efficiency for reactions of up to 45 samples.

Scitek Australia Pty Ltd

www.scitek.com.au

Handheld airborne particle counterThe Particles Plus 8206 Handheld

Particle Counter measures 0.2

to 10.0 µm with a flow rate of

2.83 L/min. Easy to configure,

the instrument displays up to six

user-selectable size channels as

well as temperature and relative

humidity.

The user can view data and

generate ISO 14644-1, EU GMP

Annex 1 or FS 209E reports on-

screen or via printer, USB key, real

time through its versatile output

options or export to Particles Plus

data-download software.

The counter can be controlled

and monitored remotely via web

browser. It also has a mass con-

centration mode that approximates

density in µg/m3. The product

meets ISO 21501-4 and JIS B9921

and ensures compliance with an

onboard pulse height analyser.

Particle & Surface Sciences Pty Ltd

www.pss.aus.net

http://www.edmundoptics.com/au


NAMSA Biological Indicators provide a convenient, easy-to-use system for the reliable validation and monitoring of your sterilisation processes including Steam, Ethylene Oxide, Radiation, Hydrogen Peroxide and Dry Heat. Available in:

Ph: (02) 9484-0931 | Fax: (02) 9980-2162 | Email: [email protected]

www.bionovuslifesciences.com.au

BIOLOGICAL AND CHEMICAL INDICATORS FOR STERILISATION MONITORING

NAMSA Chemical Process Indicators (CPI’s) are available for monitoring all common sterilisation process and are available in rolls of dots, rolls for labeling guns and in custom designs tailored your specific requirements.

• Spore strips and discs (paper, glass fibre, SS), SS wires, cotton threads

• Spore suspensions

• Spore Ampoules• Self-Contained Biological Indicators

(SCBIs)

Rapid carbapenemase screening kitRapid Carb Screen, from Rosco Diagnostics, can detect carbapen-

emase activity direct from fresh cultures (including blood culture) or

urine in less than 2 h by detecting hydrolysis of the beta-lactam ring

of a carbapenem in the presence of an indicator. The test requires

metabolically active organisms to be lysed then reacted with a tablet

containing imipenem and an indicator. The presence of a carbapenemase

is indicated by a colour change from red to yellow. A negative control

tablet is supplied to help interpret weak colour changes.

Dutec Diagnostics Pty Ltd

www.dutecdiagnostics.net.au

Conical rotorDesigned for applications that use common

disposable conical tubes, the JA-14.50 rotor

from Beckman Coulter Life Sciences extends

the applications and flexibility of the Avanti high-

performance centrifuge series.

Delivering a top speed of 14,000 rpm and a

G-force of 35,000 x g, the rotor holds up to 16 x

50 mL conical tubes, thus maximising the number

of 50 mL conical tubes that can be processed in

a single fixed-angle rotor centrifugation run. It is

said to be the only high-performance rotor capable

of spinning 50, 15, 5 and 1.5 mL conical tubes,

and 50, 15 and 10 mL round bottom tubes and

bottles, at their maximum speeds.

The rotor is intended for general separa-

tion and pelleting of cells, bacteria and food

products, proteins, viruses, subcellular frac-

tions, running concentrators and columns

and phase separation and binding studies.

A BioCertified dual-locking lid configuration, for

workflows that include biohazardous samples,

maintains biocontainment when removing the

rotor from the centrifuge and transporting it to a

containment hood. Rotors are also available in a

single-locking lid configuration.

Beckman Coulter Australia

www.beckmancoulter.com

ColumnsWaters has expanded its family

of Cortecs columns with the 2.7 mi-

cron silica-based, solid-core particle columns. Designed for

analytical scientists who need to maximise performance on their existing LC systems,

Cortecs 2.7 micron columns run at low pressures while delivering high efficiencies. This

gives the user the flexibility to use longer column lengths to improve resolution or higher

flow rates to speed instrument analysis times and increase throughput.

Available in C18+, C18 and HILIC chemistries, the company offers the columns in 48

column configurations. The columns are fully scalable to Cortecs 1.6 micron columns

for ultraperformance LC (UPLC), thus allowing separation scientists who currently run

HPLC separations the ability to futureproof their laboratories and transfer or migrate

their separation to UPLC.

C18+ columns deliver good peak shapes for basic compounds and impart se-

lectivity while being a low-bleed, formic acid-friendly option for LC/MS applications.

C18 columns are general-purpose, high-efficiency RP columns offering balanced retention

of acids, bases, and neutrals at low and mid-range pH. They provide good resolution

and retention for complex mixtures.

HILIC columns are designed for the retention of extremely polar analytes using

hydrophilic interaction chromatography (HILIC). Additional benefits include increased

mass spectrometry response and compatibility with direct injection of organic extracts

from common sample preparation techniques, such as solid-phase extraction (SPE),

liquid-liquid extraction (LLE) and protein precipitation (PPT).

Waters Australia Pty Ltd

www.waters.com

http://www.kartell.com.au

http://www.bionovuslifesciences.com.au


Consumers in Australia have adopted the web as the go-to resource to find the best price on products from around the world. Jump online, search for the product, read the reviews, watch the video, check the price and place your order - very quick and simple, plus you save money.

In 2013, Australians spent $37.1 billion online and 9% of these purchases were for medical equipment1. So while we shop online for ourselves looking for a bargain, are we doing this in the laboratory?

Why are some organisations not leveraging the power of the web? They could have saved thousands of dollars on equipment last year and stretched that research grant that extra bit further. Let’s debunk some of reasons why organisations didn’t buy online and missed out on the savings.

Reason 1: When you buy online, you don’t get the same level of support.This is a sales rep’s go-to line when trying to convince you to not buy an identical product online: “Yes it’s cheaper online, but what about support?” Let’s assess the actual reality of this throwaway line.

Scenario A: You paid more and bought the equipment from the sales rep. Your equipment goes down:

• You contact the sales rep to let them know your problem.• The sales rep is out on the road and promises to get back to

you when they return to the office.• Once back in the office (assuming they remember to call you

back), the reps asks for copies of your order details, date you purchased, how much you paid, proof of purchase, copy of invoice etc.

• You spend a day trying to find this information.• Once the rep receives the info they need from you, they for-

ward the information to the manufacturer.• The instrument is replaced or repaired under warranty, or a

contractor is sent to your site for a chargeable service job. You pay a premium for the repair, so that the original company who sold you the equipment covers the contractor’s cost and makes some money as well.

Scenario B: You paid a lot less and bought the equipment online. Your equipment goes down:

• You go to the website where you purchased the product. All of your order details are saved online against your profile. The website has a live chat feature, so you talk to a customer service person straight away.

• They have records of your entire purchase, so this information is available immediately.

• The website offers to repair or replace equipment under war-ranty or, if outside of warranty, recommends you a service contractor. You pay the contractor directly, saving on service.

Reason 2: When you buy online, you might receive a fake product or inferior quality.Being selective about which sites you source equipment from is important. Just like normal shopping, the internet is host to vary-ing quality and dishonest practices. To ensure your purchases are secure, follow these steps:

• Buy from an Australian-registered web domain. A website with ‘com.au’ in the address must have an ABN to register this web address. The laws and regulations that govern all Australian businesses, including fair trade, warranty obligations and restrictions against the supply of fake or inferior products, extend online as well.

• Shop on secured e-commerce sites. If a website is secured, the web address in the browser will begin with HTTPS:// instead of HTTP://. You should also see a small padlock icon on the top left or bottom right of the page depending on your browser.

• Ask the website to complete your organisation’s standard sup-plier form. Websites should be able to provide references for other Australian businesses they have worked with.

Next time your laboratory is looking to make a purchase, make sure you jump online and see how much you could be saving.

LabFriend www.labfriend.com.au

E-commerce in the lab Karl Wyzenbeek, Managing Director, LabFriend

1 http://www.e-commercefacts.com/background/2012/08/e-commerce-australia/

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Mass spectrometry sample preparation productsTecan offers a range of laboratory automation

solutions for mass spectrometry (MS) sample

preparation, ensuring there is a system to meet us-

ers’ workflow and throughput needs. From solid phase

extraction and liquid-liquid extraction to protein precipitation

and straightforward ‘dilute and shoot’ methods, Tecan provides

automation of routine sample preparation applications, helping to alleviate bottlenecks

for many laboratories.

Recent advances in MS instrumentation have led to an increasing number of laboratories

choosing this technology for a wide range of applications. However, sample preparation

techniques have not enjoyed the same rate of development, leading to processing bot-

tlenecks and reducing overall productivity. Automation of routine sample preparation can

alleviate these problems, reducing hands-on time and increasing throughput.

Tecan delivers automated sample preparation solutions for virtually any application,

including drug discovery, clinical, toxicological, food and environmental laboratories. The

company offers an extensive range of systems and modules to match users’ laboratory

workflow needs - from straightforward ‘pipette and shake’ sample extraction using the

AC Extraction Plate to walkaway automation with a modular Freedom EVO workstation.

Tecan Australia

www.tecan.com.au

Protocol for purification of recombinant proteinsMerck Millipore has introduced a recombinant

protein purification protocol that combines lysis

and purification into a single step. The condensed

workflow can be completed in about 45 min,

which is said to reduce processing time by 75%

compared to traditional techniques while delivering

high yield and purity.

The protocol is claimed to require less hands-

on time than the conventional workflow, providing

greater consistency and results in more reproducible yields. The method uses a spe-

cialised lysis buffer in combination with magnetic affinity capture beads, which together

eliminate the need to clarify lysates by centrifugation. As a result, the workflow can be

automated using a high-throughput particle processor, further reducing sample variability.

The one-step lysis and purification protocol has been validated specifically for

Merck Millipore’s PureProteome Nickel Magnetic Beads, optimised for the capture of

histidine-tagged proteins, and Merck Millipore’s BugBuster Master Mix, a lysis reagent

that allows for non-mechanical extraction of soluble protein from bacterial cells. The

reagent combines detergent-based lysis with enzymatic agents, Benzonase nuclease

and rLysozyme enzyme in a ready-to-use formulation.

Merck Pty Limited

www.merck.com.au

Compact mass spectrometerThe Advion Expression L CMS

compact mass spectrometer of-

fers extended range analysis,

covering a mass range from 1 to

2000 mass units with a resolution

of better than 1 mass unit. While

initially designed as a personal

mass spectrometer for organic

synthetic labs, the product has

been expanded to handle analyses

ranging from traditional synthesis

to peptide analysis and a host of

other small molecule applications.

The compact design of the

instrument allows it to be used as

a personal mass spectrometer in

the researcher’s own lab to allow

instant analysis of reaction prod-

ucts or separation components.

This speeds up the research pro-

cess as it avoids delays inherent

in waiting for central mass spec

lab analysis.

The design of the product

means it can be used in stand-

alone mode for single injection

analysis, or coupled to HPLC

systems to act as an inline mass

spec detector. The system can

also be coupled to a host of

other flow systems or separation

systems for inline analysis. It is

available with either ESI or APCI

inlet capabilities.

Scientifix-Techcomp Pty Ltd

www.scientifix.com.au


Gas chromatographThe Danalyzer 370XA gas chromatograph (GC) provides

accurate measurement, minimal maintenance and

ease of use. The product is designed for continuous

online analysis of natural gas for applications such as

custody transfer, power generation and burner fuel/air

ratio control.

The unit provides a C6+ analysis similar to legacy

Danalyzer GCs in a smaller and simpler form. New

and experienced users are expected to appreciate

the advances in technology, improved diagnostics and

intuitive local operator interface (LOI).

The product’s Maintainable Module incorporates

the analytical components in an easily removable

module. The module can be replaced in the field in

approximately 2 h (including warm-up and purge) and

can be maintained at the component level for repair.

Easy-to-use software assistants on the full-colour LCD

local operator interface reduce the need for specialised

training by providing a step-by-step walkthrough of

common operational and maintenance functions such

as changing calibration gas, auto-valve timing and

module replacement.

The product can be pole, wall or floor mounted. No

shelter is required for most environments.

Emerson Process Management

www.ap.emersonprocess.com

Brushless DC flat motors with integrated encoderBrushless flat motors from maxon motor

feature pancake form factor and torque

ratings. The product’s integrated mile encoder

maintains the motor’s low profile and includes

high-resolution encoder feedback.

The encoder operates by measuring the fields gener-

ated by eddy currents in a target disc. This is claimed to

offer improvements over traditional encoder technology

in the form of environmental robustness, improved

speed capabilities and an increase tolerance against

external interference. The integrated encoder, when

used with a matching motor position control unit, will

control the unit to resolutions of up to 25,600 qc/rev.

There are three power levels - 30, 50 and 70 W - in a 45 mm diam-

eter, and also a high-torque 90 W version in a 90 mm diameter. The

45 mm version is available with both PCB-mounted connectors or a

smaller footprint cable version. The product is suitable for driving loads

directly with high degrees of speed and position control in robotics and

factory automation applications.

maxon motor Australia Pty Ltd

www.maxonmotor.com.au

RheometerThe Thermo Scientific HAAKE Viscotester iQ Rheometer enables users

to measure and analyse the rheological properties of a wide range

of different samples, increasing efficiency and minimising the risk of

operator error. Designed for intuitive operation, the rheometer adapts

to individual measurement requirements in laboratories focused on

quality control.

The instrument is suitable for simple viscosity determination as well

as complex rheological investigations from low viscous liquids to stiff

pastes and semisolids. Used as a standalone unit or fully software

controlled, its compact size and small footprint makes the product a

portable unit for mobile use or a major tool in quality control laboratories.

It is suitable for quality control in the food industry; mineral slur-

ries; pharmaceutical and cosmetics; petrochemicals; polymers; and

surface coatings.

Rheology Solutions Pty Ltd

www.rheologysolutions.com

http://www.storemasta.com.au


Exotic particle confirmed by CERNScientists at CERN’s Large Hadron Collider (LHC) have confirmed the existence of a new class of subatomic particles called exotic hadrons. The LHCb collaboration, one of the four large experiments at the LHC, found that the exotic object does not fit into the pattern of particles observed up until now.

Hadrons are subatomic particles that can take part in the force that binds protons inside the nuclei of atoms. The protons and neutrons that form atomic nuclei consist of particles, called quarks, bound together. A subset of had-

rons, called mesons, is formed from quark-antiquark pairs, while the rest - baryons - are made up of three quarks.

However, the underlying theory of quantum chromodynamics (QCD) that describes the behaviour of quarks allows for many different quark combinations, such as four quark states, to bind together into hadrons. Many searches for such exotic states have been performed, with one of the most recent experiments conducted by the Belle Collaboration in 2008. At that time Belle reported evidence for an exotic structure, the Z(4430)-, that did not fit into the normal classification scheme, but the observation was questioned. The existence of the Z(4430)- particle has now been confirmed, with detailed studies showing that the LHCb data can only be explained by the inclusion of the Z(4430)-. This state shows behaviour that is characteristic of a resonance (‘phase motion across the peak’).

“We have now undisputable proof that nature is actually able to form more complex, more sophisticated objects of quarks than what was initially proposed by Gell-Man in 1964,” said LHCb physicist Richard Jacobsson.

This image shows the so-called Argand diagram proving to the experts that the Z(4430)- structure seen in the data (black points) represents really the resonant particle production and decay, since it follows approximately a circular path (red circle).

A view of the LHCb experiment at under-ground Point 8 on the LHC. The prominent tube is the LHC beam pipe, in which protons circulate at close to the speed of light. Image: Anna Pantelia/CERN.

The team analysed 25,000 interesting decays of neutral B mesons selected from data from 180 trillion proton-proton collisions in the LHC. Jacobsson explained that the B meson decayed the three particles, and they analysed the outcome to see if an object would be formed in the intermediate stage in the decay process.

“What we have seen is that nature is actually forming an object of four quarks,” he said.

The researchers’ next steps are to search for other signs of the particle in other decays of B hadrons so they can further study its properties. This may give signs of this same particle, allowing com-plementary ways to understand the nature of this state and what it really is.

“LHCb’s observation and measurement of the Z(4430)- is going to help us explore this feature of matter,” noted Professor Tara Shears, LHCb lead for the University of Liverpool. Jacobsson further noted that understanding the dynamics of the strong force could help in our comprehension of, for example, the compositeness of a neutron star.

“LHCb’s measurement also demonstrates the experiment’s versatility,” Professor Shears added. “Who would have thought that an experiment designed to investigate the strange features of antimatter could also help us understand QCD and matter better?”

The researchers’ results were presented on 8 April at the SM@LHC conference in Madrid, Spain. They can be viewed online at http://arxiv.org/abs/1404.1903.

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Digital benchtop refractometerRudolph Research Analytical has developed a refractometer with operational

features that help users load and monitor sample quality. The J457 comes

equipped with Smart Measure technology that automatically detects when the

prism is improperly cleaned, insufficient sample is loaded or the instrument is

improperly calibrated. If the prism surface does not produce the correct ‘Clean

Prism’ result, Smart Measure notifies the operator that further cleaning of the

prism is necessary. Only once the prism is properly cleaned will the operator

be notified that the product is ready to measure. If the metrics indicate that

improper sample loading has occurred, Smart Measure will direct the operator

to clean the prism and reload the sample. Operator judgement is not required

and eyeball alone assessments of sample load quality are eliminated.

Smart Measure is constantly scanning the prism surface and monitoring

the value measured against its stored database of results. If during a water

zero the instrument detects a value that is too different from the database or

the Refractive Index value being input is far from the measured value, it will

alert the operator that the instrument needs further calibration or cleaning.

Scientific Solutions Pty Ltd

www.scisol.com.au

Assay for postnatal detection of DNA CNVsThe Affymetrix CytoScan Dx Assay is intended for the postnatal detection of DNA

copy number variants (CNV) in patients referred for chromosomal testing. It is

designed to help physicians diagnose children’s developmental and intellectual

disabilities more comprehensively by enabling a high-resolution genome-wide

analysis of genetic aberrations.

The product analyses the whole genome in a single assay at very good

resolution, improving diagnostic capability. High-resolution analysis can reveal

small aberrations not readily seen using traditional techniques. The company’s

For Research Use Only version of the assay is currently available in Australia

for both constitutional and cancer cytogenetics research applications.

Millennium Science Pty Ltd

www.mscience.com.au

Haematology analyserThe Diatron Group has launched an updated version

of its Abacus 3CT haematology analyser. The product

is a compact, benchtop, flexible, three-part differential

analyser which now offers 22 parameters to deliver even

more patient data. With its cap piercing capability, it can

be used with sample tubes that are open or closed for

greater convenience.

The most visually apparent change is the introduction

of the 8″ high-resolution, colour touch screen with an

intuitive multilingual user interface, making it clear and

simple to operate. The product also has an enhanced

package of onboard quality control (QC) features. The

QC capacity has been greatly increased to 24 lots and

QC values can be loaded via QR codes or USB to avoid

any incorrect assignment of values.

Operating rights can be assigned to selected operators

to ensure only trained persons can use the system. In

addition, an onboard log details when reagents were last

changed, with a constant on-screen display of reagent

expiry dates as well as remaining reagent volumes. Addi-

tional QC features, such as aperture anticlog protection and

automatic cleaning after every 15 sample measurements,

ensure the quality and reliability of diagnostic results.

To accommodate the increased volume of data generated

by the 22 parameter measurements, the onboard data

storage capacity has been doubled to 10,000 records,

with improved LIS connectivity for results transfer. The

latest features make the product easy to use and suit-

able for small laboratories, satellite clinics and doctors’

offices alike.

Diatron Group

www.diatron.com

http://www.labtek.com.au


my

lab Hunting for breast cancer risk

with next-gen sequencing

The Hunter Medical Research Institute’s (HMRI) Pathology Service laboratory has developed DNA sequenc-ing technology that is accelerating screening for the genetic risk of breast and ovarian cancer.

Professor Rodney Scott, the co-leader of HMRI’s Information Based Medicine research program, ex-plained that the institute acquired funding to purchase a next-generation sequencing (NGS) system from genetics technology company Illumina. With the assistance of Dr Cliff Meldrum from The University of Melbourne, the NGS technology was married to microfluidics.

“The reason we had to do that is because for next-gen sequencing we need to amplify targeted re-gions that you want to interrogate, ’cos you can’t just take DNA from somebody and, without any sort of amplification, interrogate it,” Professor Scott stated.

This allowed the researchers to get a comprehensive amplification of two major genes associated with breast cancer risk - BRCA1 and BRCA2. From there, they could screen the fragments that had been amplified and look for genetic changes in them.

“The two genes, BRCA 1 or BRCA 2, are both large, so we break them into around 180 fragments and find the DNA sequence of each fragment,” Professor Scott said. “We then reassemble them to make the total sequence.”

The technique is much faster than the traditional ‘gold standard’ method of Sanger sequencing, which has been in use since 1975. From a single reaction, said Professor Scott, 42 samples can be screened for both genes within 24 hours - as opposed to taking 2-3 weeks previously.

“The additional advantage of next-gen sequencing technology is the speed at which you can analyse the data, which cuts it down dramatically from several days to within about 50 minutes, thereby increas-ing the number of samples that you could look at at any one time and report on in that time frame.”

The method received NATA (National Association of Testing Authorities) accreditation towards the end of 2013, when the lab tested its bank of around 500 samples and matched the results with its previ-ous data. Six months later, it has been used to screen over 1000 more women for their genetic risk of breast cancer.

“We could in fact do more - the only thing that’s stopping us is actually the supply of samples,” Pro-fessor Scott said.

As the team continues to screen patients for ovarian and breast cancer risk - about 100 samples per month - they are also hoping to broaden the method for use in other diseases.

“It has the potential to alter the way we provide medical services, in terms of identifying people at risk as opposed to reacting to people who present with disease, so that you can begin to

think about seriously offering preventative measures to people,” Professor Scott said.

Lauren Davis

Wrapper number:(if known)

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What matters to you most in an analytical lab?Delivering reliable results, following regulations whilst supporting a sustainable environment.

Our portfolioAnalytical chemistry

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Suprapur® and Ultrapur® high purity acids and bases: optimal purity for sample preparation

Certipur, Chromolith, Milliflex, Milli-Q, Spectroquant, Suprapur, SupraSolv, and UltraPur are registered trademarks of Merck KGaA, Darmstadt, Germany. Merck Millipore, and the M mark are trademarks of Merck KGaA, Darmstadt, Germany. © 2012 Merck KGaA, Darmstadt, Germany. All rights reserved.

How do you get there?We know how.With a complete and sustainable solution.Detailed, reliable testing methods, trusted analytical reagents as well as chemicals for quality control and research are key for achieving your goals. Our range of analytical products ensures reproducible results utilizing state-of the-art methods. Our extensive documenta-tion and training materials help you constantly comply with current regulatory requirements. Product take-back programs, sustainable design and packaging as well as our strict energy management are just some solutions which help you meet your internal sustainability priorities.

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Benchtop Cell Culture Aspiration Station

New portable aspiration Systems from Rocker,

ideal for single and multi-channel applications

Includes integrated piston vacuum source, suction kit - with single and 8-channel suction heads - & 1200ml autoclavable PES waste bottle.

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RLF10000 BioDolphin light blue suction kit - please refer to Kit accessories page

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PowerReady

Australianstandards

Accesories

• Ideal to be used in Vacuum Aspiration & Cell Culture Aspiration

• Includes a safe valve for stopping f luids once levels are aproaching the limits of the tank.

• The unique Labf il400 f iltration system combines vacuum source and f iltration into one unit.

• Robust and quiet device with minimal footprint.

for full Specssimply

Click here

https://www.pacificlab.com.au/


http://www.pacificlab.com.au/media/files/pacificlab-rocker-vacuum-systems-web.pdf

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www.pacif iclab.com.auM E L B O U R N E S Y D N E Y B R I S B A N E A D E L A I D E P E R T H A U C K L A N D. . . . .

Features:• 360° Rotation• Mixing angle adjustable to 0-90°• Interchangeable rotisserie• Cold room and incubator compatible• Easy to use / angle change• No tools required

$ 4 8 0 *Only Revolver Rotator TM H5600

Prism MiniMicrocentrifuge

Part NumberH5600-230V-UK

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Part NumberC1801-230V-UK

To receive discount please quote the following Promotion Code: when Ordering. LAB-0814-2

Features:• Intuitive design• Self opening lid enables single-handed operation• Quick release rotor hub• Built in braking system for rapid sample processing• Easy to use• No tools required

The ef f icient H5600 allows to mix horizontally, vertically or any position in between.

Compatible with a wide variety of tubes sizes.

Compact and economical personal device ideal for molecular separation or just any quick spin.

$295 *Special price

To receive discount please quote the following Promotion Code: when Ordering. LAB-0814-2

Labnet International, Inc.

Page 2

Code No Description

Speed Range Fixed, 20 rpm

Rotation axis Continuously adjustable, 0 - 90°

Ambient temp. +4° to 65°C

Dimensions 9.4 x 5 x 6 in/23.9 x 12.7 x 15.24 cm (W x D x H)

Weight 4.4 lb/2.0 kg

Specif ications

* Includes two quick release interchangeable rotors.

12Months

Warranty


Click here


https://www.pacificlab.com.au/shop/products/h5600-230v-uk-revolver-tube-mixer-w-rotisserie-for-36-x-1-5-2-0-ml-tubes/1/name



https://www.pacificlab.com.au/media/products/200521.pdf

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Part NumberR-96-PCR-FB

To receive discount please quote the following Promotion Code: when Ordering. AXY-0814-1

• Improved Ergonomics

• Reliable Accuracy

• High Precision

• Fast volume setting

• Volume locking system

• Easy maintenance

• 4-digit counter

• Autoclavable

96-Well PCR Tube Rack with LidIdeal for Storage and Freezing

The 96 well racks are also available in assorted colours.

Starter Pack Includes, Part No. 7903 Universal 4-position stand, Tips 10µl, 96 pcs, Tips 200µl, 96 pcs, Tips 1200µl, 96 pcs, Calibration tool x 3, Pipette hanger x 3, Instruction manuals.

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Discovery Pro Pipettes

PCR Tube Storage Rack

Single ChannelStarter Pack

Product Information

6001 0.2 - 2.0 µL Single Channel

6002 0.5 - 10 µL Single Channel

6003 2 - 20 µL Single Channel



6006 100 - 1000 µL Single ChannelTo receive discount please quote the following Promotion Code: when Ordering. LAB-0814-2

$ 950 *Just

4 Pipettes+stand

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..

..

..

..

..

..

..

..

..

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* AUD$225* Offer applies to any of the Single Channel Pipettes listed on the table above.

$225 *Now

Save up to $72.each

00

Page 3

$17 *Only

5 pack

. 0 0


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$ 949 *Reduced to

Part NumberZSXH-618

Part NumberZWQ-344

Part NumberZWF-334

Part NumberZWYR-211C

Part NumberZWYR-D2402

To receive discount please quote the following Promotion Code: when Ordering. LBW-0814-1

For detailed information regarding the Ex-Demo Clerance Sale, please contact one of our product specialists at: [email protected]

Premium Stackable Shaking IncubatorPremium Horizontal Shaking IncubatorBenchtop Shaker - LinearBenchtop Shaker - Rocker

High quality #304 stainless steel for excellent durability of interiors and its parts.

30L and 50L capacity reciprocal shaking water baths are also available.

Water BathCirculating

Labwit waterbaths deliver superior temperature control and uniformity,Ideal to be used in the Biology, Pharmacy and Chemical industry.

• 18L tank volume with diffuser platform, temperature control from ambient-99.9°C

• LCD digital controller provides a user-friendly interface

• Circulating pumping system ensures highly uniformed temperature distribution

• Stainless steel hinged gable lid prevents uncontrolled heat dissipation

• Low water level alarm and non-volatile memory

Ready to shipIn Stock!



. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Ex-DisplayClearance

SALEGreat deals on Labwit laboratory equipment

Buy Now and Save!

Page 4

18Months

Warranty

PowerReady

Australianstandards


Click here


https://www.pacificlab.com.au/shop/products/zsxh-618-water-bath-18l-with-pump-a-65/1/name


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mailto:[email protected]




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2Years

Warranty


• High Speed, up to 20.804x g./14.300 RPM• Rotor list on memory• Up to 175 acceleration ramps and up to 175 braking ramps• Easy to read TFT touch screen • Automatic rotor recognition• Microprocessor controlled• Automatic open lid• Lid latch lock• Adjustable acceleration and braking• 15 memories + pre-cooling program

• Speed, up to 24.959 x g/16.500 RPM• Quiet noise level < 60 dB• Capacity Max .4 x 100 ml• Swing out rotors for max 4 x 100ml for tubes, microplates and microtubes• Angle f ixed low speed up to 24 tubes of 15ml• Angle f ixed low speed for microtubes up to 50 ml conical• 16 memories• Automatic open lid

Part NumberCE114

Part NumberCE110


To receive discount please quote the following Promotion Code: when Ordering. Prices do not include rotors or buckets.ORT-0814-1

High Performance CentrifugesSafe , Robust and Reliable

Consul 21R

Digicen 21

alresa

OA GREEN TECHNOLOGY

* 95% recyclable parts used in fabrication.* Incorporating HFC gases in refrigeration where used reducing impact on the earth’s ozone hole.

$2,999 *Special price


Features:

Features:

Extensive range of rotors

and accesories for multiple

combinations



Page 5

Centrifuge Tubes

also available

Made inEurope


Click here


Click here


https://www.pacificlab.com.au/shop/tubes-centrifuge-1/we4g-tube-centrifuge-15ml-p-p-grad-conical-blue-cap/1/name

https://www.pacificlab.com.au/shop/centrifuges-1/ce110-digicen-21-230-220-v-50-60-hz/1/name

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https://www.pacificlab.com.au/media/files/ortoalresa-plp-catalogue-web.pdf




https://www.pacificlab.com.au/media/files/ortoalresa-plp-catalogue-web.pdf


• Electrical, no gas needed, no open f lame• Instant-ready-to-use, no warm-up time, no waiting• Sterilization temperature 900 – 1,300 °C, 1,650 – 2,370 °F• Touch-free START, with IR- sensor and integrated timer• Economical, low-cost operation

Solaris Loop Sterilizer

Sterilization of inoculating loops, tweezers and small instruments within seconds.

Temperature 900 °C . 1.300 °C

Safety class IP 20

Connected voltage 100-120 VAC bzw. 200-240 VAC, 50-60 Hz, 500 W Short-time

Technical data

Dimensions (w x h x d) 152 x 210 x 195 mm

Weight approx. 2.500 g

12Months

Warranty

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Part Number3399002

To receive discount please quote the following Promotion Code: when Ordering. SCH-0814-1

Page 6

Made inEurope


Click here


https://www.pacificlab.com.au/shop/products/3399002-solaris-loop-sterilizer-240v/1/name






Spatula Spoon SS 150mmSpatula Spoon SS 250mm

Spatula flat both ends SS 150mm

Spatula chattaway 155 x 8.5mm SSSpatula chattaway 210 x 8.5mm SS

Part Number374.303.07

Part Number

374.303.02374.303.04

Part Number

375.303.01375.303.02

SpatulasMade of heavy Stainless Steel grade 304/316

$8.50 each$9.50 each

$8.50 each

$8.50 each$9.75 each

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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Page 7

Great Value for Money!

Product Information

139.202.01 5 ml Measuring Cylinder Round Base

139.202.01A 10ml x 0.2ml class A blue grad. DIN

139.202.03A 50ml x 1ml class A blue grad. DIN



Product Information - USP Standard

058.202.04 100ml 24/29 ISO 4797

058.202.12 250ml 24/29 ISO 4797

058.202.16 500ml 24/29 ISO 4797

058.202.20 1000ml 29/32 ISO 4797

Product Information

071.202.09 50ml 19/26 ISO 4797 & USP

071.202.10 50ml Conical 24/29

071.202.12 100ml 19/26 ISO 4797 & USP

071.202.13 100ml 24/29 ISO 4797 & USP

071.202.19 250ml 24/29 ISO 4797 & USP

071.202.22A 500ml 24/29 ISO 4797 & USP

Interchangeable / General Glassware

Cylinders ErlenmeyerFlat Bottom

Stunning performance at af fordable

prices

Joints, stoppers, adapters and connectors also avalable

Request a completeGlassware catalogue

simply contact: sales@pacif iclab.com.au


https://www.pacificlab.com.au/shop/flasks/058-202-04-flask-flat-bottom-100ml-24-29-iso-4797-and-usp-standard/1/name

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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Terms and Conditions

FP-F

L-060

614A

U

Sterile SwabsIdeal for microbiological sample collection

Part Number300287

Swab plain PS and cotton tip with tubeSterilised by ethylene oxide

Sterilised by radiationSwab aimies PS and cotton tip with tube

Part Number300261

$85 *Only

$150 *Only

per 500

To receive discount please quote the following Promotion Code: when Ordering. DEL-0814-1

per 100

Page 8

Inoculating Loops

Spreader Bars

Polypropylene

1ul Loop Clear

10ul Loop Blue

Clear, Gamma Sterile

Polypropylene

Part NumberP10805050



1 0 %Special Price

Off

To receive discount please quote the following Promotion Code: when Ordering. TCH-0814-1

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Australian Head Of f ice

PO BOX 465 Blackburn, VIC 3130

Free Call: 1800 723 405Ph: (03) 9845 0300

Fax: + 61 3 9845 0350e-mail: sales@pacif iclab.com.au

Connect with us:

/ Pacif icLab

@ Pacif icLabP

* Prices quoted in AUD exclude GST and delivery charges

* Featured Products promotions are valid until the 31st of August 2014 Only.Promotions cannot be used in conjuction with any other offer.Offers exclude spare parts, servicing, freight, handling and are available while stocks last only, no rain-checks. Offers are not available for resellers. Prices apply to stock “On Hand” Only. Pacif ic Laboratory Products Pty Ltd reserves the right to modify or withdraw this of fer at any time.


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Documents

What’s New in LAB & Life Sciences Jun/Jul 2014