Welcome to Cell Line Vials Maintenance Repository

Developed by

Ojaswini Upasani

Anand Jadhav

Nishigandha Naik

ACTREC, Navi Mumbai

Cell Line Vials Maintenance

Canister

Cane

Box

Vials

Cylinder

CLVM

Cell Line Vial Maintenance

Program

What is CLVM Program? Who can use this? Why to use this?

Let us know one by one…..

This is a Cell Line Vial Maintenance (CLVM) repository provides information about the cell lines stored in liquid nitrogen cylinders. The information includes locations of the vials containing cell line of interest in various cylinders, freezing records of the vials like medium used for freezing, date of freezing, etc. and conditions used for maintenance of the cell line. Moreover, listing of the stored cell lines can be obtained on the basis of cell type, tissue of origin and species origin. It also indicates the empty positions available for storing freezing vials.

What is CLVM Program?

Any laboratory where tissue or cell culture workis a routine activity and needs to keep a track of stock for longer period can use this CLVM software.

Who can use CLVM Program?

Traditionally, records of frozen vials of various cell lines are maintained in the files or record books. To search for a particular vial of interest or to check the earlier records for comparison or for stock checking, one has to screen through several pages of the book. This is very tedious and time consuming. CLVM saves time and efforts and provides more information.

Why to use CLVM Program?

Now let us check the programme…..

Data Entry

Cell line Vial Data Maintenance Record – Main Menu

Data Entry

Vial Revival

Build Index

Reports

Exit

Frozen Vial listRevival of Cell linesNumber of Vials in Cane / BoxEmpty positions in Cane/BoxCell lines Data Sub-ListsReturn to Main

Name wise listTissue wise listType wise listOrigin wise listReturn to Main

Highlight option with using → or ← and press or press

appropriate highlighted character. Press F1 for Help About the Program

Cell Line details will cover

Cell line Name and User

Type, Tissue, Origin, Media, Percentage of Serum, Antibiotics, Conditioned media if any etc.

Freezing details – Date, Freezing media, serum, User, Passage/batch, Special remarks, etc.

Actual location details of Particular Vial in Cylinder, Canister, Cane/Box along with its position number.

Important Modules :

Data Entry for New Cell Line along with Vial position details

Cell Line Revival

Various Reports –

Stock frozen vials positions - cell line wise

Archival Records for Revived cell lines

Finding empty positions/Occupied positions in Cane/Box

List of Cell lines – Name/Type/Tissue/Origin wise

This module handles data entry. The data includes names of the cell line and user, detailed information about the cell line, i.e., cell type, tissue and species, maintenance and freezing media etc. Most of the information can be added by drop down choose menu. This reduces data entry work. If multiple vials of one cell line are prepared on the same date by the same user, then option is provided to choose the cylinder, canister and position for multiple vials.

ADD MODULE

II VI Box 33 5

Revival module provides help for revival of a particular cell line. Location of the vial and detailed information of the desired cell line can be precisely checked. Once the chosen vial is marked by entering revival date i.e. “Rev. dt” and “Revived by”, it updates record such that the marked vial position is now shown empty to add new one.

REVIVAL MODULE

Revival module

List of Cell line: Name wise Cell Line Cell Type Tissue Origin 32DCBCR/ABL Myeloid Haematopoietic Mouse 32DCL3 Myeloid Haematopoietic Mouse FBM-E4 Epithelial cells Buccal mucosa Human FBM-SR18(1) Epithelial cells Buccal mucosa Human H9 Lymphocytic Haematopoietic Human HELA Epithelial cells Cervix Human HL-60 Promyelocytic Haematopoietic Human K562 Myeloid Haematopoietic Human MCF7 Epithelial cells Breast Human RIN/FPR Epithelial cells Insulinoma Pancreas Rats U-937 Myelomonocytic Haematopoietic Human WEHI3 Lymphocytic Haematopoietic Human { End of File Encountered! Press any key to exit... }  

Sample Report : Name wise Cell Line List

Other applications

• Cell Line Repositories

• Tissue Culture Laboratory

• Blood Bank

Advantages of programme

Restricting Unauthorized Access Representing Data as per requirements – e.g. variety

of lists Controlling Redundancy Providing Backup Reduced Record Maintenance Time Standard can be enforced Flexibility Availability of up-to date information

THANK YOU

Contact details for this Programme:Advanced Centre for Treatment, Research & Education(ACTREC)Tata Memorial Centre, Sector 22, Kharghar, Navi Mumbai 410208Tel:022-27405039 Fax: 022-27412892 E-mail:nnaik@actrec.gov.in