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Biological Responses can be measured at different levels of organisation:
Molecules
Cells
Individuals
Populations
Communities
• If they form part of a key function
Reproductive Capacity
Immune Function
Effects measured at molecular and cellular levels can impinge on populations
Measurement of Immune Function
Water Quality
Mechanisms of Immune Defence• Phagocytosis• Blood Cell Proliferation• Release of Antimicrobial Molecules
Apparatus for Immunity• Total Blood Cell Counts• Differential Blood Cell Counts• Haemopoietic Tissues
Efficacy of Immune Response• Susceptibility to Infection
Apparatus for Immunity• Total Blood Cell Counts• Differential Blood Cell Counts• Haemopoietic Tissues
Measurement of Immune Function
Measurement of Immune Function
Mechanisms of Immune Defence• Phagocytosis• Blood Cell Proliferation• Release of Antimicrobial Molecules
Measurement of Immune Function
Field studies have shown correlations between assays of immune function and tissue levels of specific contaminants
Measurement of Immune Function
Local studies have shown differences between immune parameters from contaminated sites compared with clean reference sites
Measurement of Immune Function
Summary of Potential in Assessing Water Quality
• A wide range of assays can measure immune function at different levels of organisation
• Laboratory and field studies have shown these assays to be responsive and to correlate with disease susceptibility
• Laboratory and field studies have shown correlations between contaminant levels and these assays
Algal Blooms
There are more than 3000 species of Marine Phytoplankton showing an enormous diversity in size and form
Algal Blooms
• Of the >3000 species at least 300 are able to form harmful blooms
• With more than 40 species able to produce biotoxins
• Studies show increasing numbers of:
toxic species
toxins produced
geographic areas affected
fisheries, aquaculture and tourism affected
higher economic losses
Algal Blooms
• Amnesic Shellfish Poisoning (ASP) causative organisms: Pseudo-nitzschia sp.
Toxin produced: Domoic Acid
ASP characterised by both gastrointestinal and neurological disorders
• Diarrhetic Shellfish Poisoning (DSP) causative organisms: Dinophysis spp.
Toxin produced: Okadaic Acid
DSP characterised by gastrointestinal symptoms.
• Paralytic Shellfish Poisoning (PSP) causative organisms: Alexandrium spp.,Gymnodinium catenatum, Pyrodinium bahamense
Toxins produced: Saxitoxins
PSP characterised by neurological symptoms
Conditions resulting from toxic algal blooms include:
Algal Blooms
Plymouth Algal Culture Collection:
• Forms a living research collection of marine microalgae
• Established by E.J. Allen and T.C. Nelson in 1910
• The main collection consists of some 250 strains from 70 genera
• In addition we hold some 250 Emiliania huxleyii clones
• The cultures are maintained in liquid culture with regular sub-culturing
• The Collection distributes approximately 400 culture strains per year