Upload
others
View
0
Download
0
Embed Size (px)
Citation preview
UNIVERSITY OF BOLOGNA
DiSTA Department of Agroenvironmental Science and Technologies
GRiCI – Research Group on Industrial Crops –Viale Fanin 44, 40127 Bologna, ITALY. Tel: 0039 051 2096652. Fax: 0039 051 2096241. G.Ri.C.I. website: www.agrsci.unibo.it/dista/grici/
BioKenaf Project Contract No: QLK5-CT2002-01729
Second Year ResultsSecond Year Results(WP2 (WP2 -- Task 2.2 _ 2.3)Task 2.2 _ 2.3)
Fifth Technical MeetingFifth Technical MeetingCataniaCatania –– July 5 July 5 -- 6, 20056, 2005
Technical staff: Technical staff: Prof Prof G.VenturiG.Venturi
Prof M.T. Prof M.T. AmaducciAmaducciDottDott. N. Di Virgilio. N. Di Virgilio
Experimental Farm of Bologna University, Cadriano (Northern Italy)
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
44° 33’ N, 11° 21’ E
altitude: 32 m a.s.l.soil slope: 0% organic matter = 1.9%pH = 7.14
33 m
3 14 4 57
6
3
14
48
35 m
3 7 4 47 7 3
hemp
fieldhemp
field
Task 2.2
Task 2.3
Block 1
Block 2
Block 3
Block 4
Block 4
Block 3
Block 2
Block 1farm soil typology:Udic Ustochreps fine silty, mixed mesic
sand = 19%silt = 53%clay = 28%
"
"
"
"
"
""
"
"
"
"
"
"
"
"" "
" ""ROMA
Bologna Experimental layout
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
1 S1 V2 D1 S2 V2 D1 S2 V2 D2 S1 V2 D1 N1 N0
2 S1 V2 D2 S2 V1 D1 S1 V1 D1 S2 V1 D2 N3 N2
3S2 V2 D2 S1 V1 D2 S1 V2 D1 S1 V2 D2 N3 N1
4 S2 V2 D1 S1 V1 D1 S2 V1 D2 S1 V1 D2 N2 N0
5 S2 V1 D2 S1 V2 D2 S2 V1 D1 S2 V2 D1 N0 N1
6 S1 V1 D2 S2 V2 D2 S2 V2 D1 S2 V2 D2 N2 N3
7 S2 V1 D1 S1 V2 D1 S1 V1 D2 S1 V1 D1 N2 N3
8 S1 V1 D1 S2 V1 D2 S1 V2 D2 S2 V1 D1 N0 N1
TASK 2.2 TASK 2.3
Block 4
Block 3
Block 1 Block 2 Block 3 Block 4
Block 2
Block 1
S1S2V1V2D1 20 p m-2
D2 40 p m-2
N0N1N2N3
100 N Kg ha-1
150 N Kg ha-1
Task 2.2
Task 2.30 N Kg ha-1
50 N Kg ha-1
Everglades41Tainung2
1st sowing time (7th May 2004)2nd sowing time (9th June 2004)
First sowing date:7th May 2004Tot. emergence:5 days
Second sowing date:9th June 2004Tot. emergence:6 days Woven non woven tissue
Irrigation after sowingto help emergence
Fertilization:4th June 2004
sowing date:21th May 2004Tot. emergence:5 days
Complete randomized block design for all tested factors
TESTED TREATMENTS AND EXPERIMENTAL LAYOUT OF Task 2.2 and Task 2.3
COLLECTED DATA
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Repeated harvests during crop cycle Repeated harvests during crop cycle
(rows of 1,5 m)(rows of 1,5 m)• Fresh / dry weight of harvested rowFresh / dry weight of harvested row
•• Number of plantsNumber of plants
•• Height (cm) and Base Stem Diameter (mm)Height (cm) and Base Stem Diameter (mm)
•• Fresh / dry weight of stems, leaves and petiolesFresh / dry weight of stems, leaves and petioles
•• Leaf area (cmLeaf area (cm22))
Meteorological data like Meteorological data like ……•• Daily maximum/minimum air temperature (Daily maximum/minimum air temperature (°°C)C)
•• Rainfall (mm)Rainfall (mm)
•• Air humidity (%)Air humidity (%)
•• Evaporation (glass A panel)Evaporation (glass A panel)
•• Sunshine hoursSunshine hours
•• Water tableWater table
Soil typical data before sowingSoil typical data before sowing
Final harvests of 5 mFinal harvests of 5 m22 (Harvest A and B)(Harvest A and B)•• Biometric parameters Biometric parameters
•• Number of branchesNumber of branches
•• Number of nodesNumber of nodes
•• DecorticationDecortication (fresh / dry weight of bark and core)(fresh / dry weight of bark and core)
•• C and N% in leaves, stems and soil (Harvest A)C and N% in leaves, stems and soil (Harvest A)
•• Ash content (%) in coreAsh content (%) in core
The light response curve of leaf photosynthesis (A)The light response curve of leaf photosynthesis (A)
I II III IV V VI VII VIII IX Xdate 18/6 1/7 15/7 29/7 12/8 2/9 20/9 13/10 17/11 14/12DOY 170 183 197 211 225 246 264 287 322 349DAE 38 51 65 79 93 114 132 155 190 217GDD 258 407 563 744 926 1175 1328 1465 1542 1542DAE 4 17 31 45 59 80 98 121 156 183GDD 35 184 341 521 703 959 1111 1248 1325 1325date 18/6 1/7 15/7 29/7 12/8 3/9 21/9 14/10 18/11 15/12DOY 170 183 197 211 225 247 265 288 323 350DAE 24 37 51 65 79 101 119 142 177 204GDD 190 338 495 676 858 1119 1269 1397 1474 1474
S2
TASK 2.3
Harvest n°
TASK 2.2
S1
COLLECTED DATA
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
4
Seeds at 10 cm within the row
4
Seeds at 5 cm within the row
Seeds at 5 cm within the row
2
STAR 1 STAR 3STAR 2
• Height (cm) and Base Stem Diameter (mm) for all plantsHeight (cm) and Base Stem Diameter (mm) for all plants
•• Fresh / dry weight Fresh / dry weight
•• Fresh / dry weight of bark at 20, 50, 150 cm from centreFresh / dry weight of bark at 20, 50, 150 cm from centre
•• % of fresh bark% of fresh bark
Effect of the distance between the rows on plant biometric paramEffect of the distance between the rows on plant biometric parameters and yieldseters and yields
Tainung2Sowing: 21st May 2004
COLLECTED DATA
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Meteorological data
0
5
10
15
20
25
30
35
40
MAY JUN JUL AUG SEP OCT NOV DEC
°C
0
10
20
30
40
50
60
70
80
mmRainfall Rainfall (10 years) T Max
T Min T mean T Max (10 years)
T min (10 years)
Rainfall, Min and Max temperature during the experimental period.
Air temperature followed the typical trend of the local conditions
Rainfalls were almost absent from August until 12th September
50 mm in one event with hail
More rainfall in May and June than 2003
Temperatures during the growing period resulted lower than 2003 mainly during August
Harvest A 195 434,4 1541,8
Harvest B 222 517,4 1541,8
Harvest A 162 385,8 1325,5
Harvest B 189 468,8 1325,5
Harvest A 182 425,2 1473,8
Harvest B 209 508,2 1473,8
TASK 2.2
TASK 2.3
Plant Cycle Lenght (Days)
acc. Rainfalls (mm)
S1
S2
acc. GDD from emerg (°C)
After the first sowing date few days without GDD increasing was observed.
GDD stopped to increase on the beginning of November, few days before harvest A. In 2003 it happens at the middle of October.
Rainfalls from the first sowing (7th May 2004) to the final harvest (15th December 2004) were 517 mm, instead of 380 mm of the last year and of 539 mm as a mean of the last 10 years
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Task 2.2 Task 2.3 units method
36 44 %
41 35 %
23 21 %
7,74 7,41 M.III. 1 DM 13-9-99
< 1 < 1 % (Dietrich-Fruehling)
< 1 < 1 % (Drouineau)
9,4 8,6 g kg-1
1,6 1,5 %
1,06 1,09 %o (Dumas)
16 12 meq/100 g (M. 27 DM 11-05-92)
182 169 g kg-1 P2O5 (Olsen)
197 215 g kg-1 K2O (M. 13,5 DM 13-9-99)
2955 2625 g kg-1 CaO (M. 13,5 DM 13-9-99)
308 380 g kg-1 MgO (M. 13,5 DM 13-9-99)
37 33 g kg-1 (M. 13,5 DM 13-9-99)Na exc.Mg exc.
CaCO3 ACT
(Walkley & Black)
Ca exc.
pH (in H20)
Organic Mattertotal N
Organic Carbon
CaCO3 TOT
sandsilt
clay
K exc.
C.E.C.P ass.
Soil analysis results of Task 2.2 and 2.3 fields
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
50
60
70
80
90
100%
Tainung2Everglades41
Sowing on 14/04/2004. First germination survey on 19/04/2004. 50 seeds per 4 replications. Incubation room at 20-30 °C.
Second germination survey on 26/4/2004.
Weight 1000 seeds:
Tainung2: 297.1 g
Everglades41: 292.2 g
20% of seed damaged by sowing 20% of seed damaged by sowing machinemachine
Adapted mechanical Adapted mechanical sowing machine sowing machine
““VignoliVignoli””
Seed germination test
5 DAS A B C D mean St. Dev. St. Err
Tainung2 78 90 74 88 82,5 7,72 3,86
Everglades41 88 90 86 98 90,5 5,26 2,63
10 DAS
Tainung2 98 92 100 100 97,5 3,79 1,89
Everglades41 94 96 90 98 94,5 3,42 1,71
96
% of germinated plants after 5 and 10 days of incubation.
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Plant germination and emergence on field
D1
D2
Task 2.3
0
5
10
15
20
25
30
35
40
45
50
1/6 1/7 31/7 30/8 29/9 29/10 28/11 28/12
Harvest date
p m -2
D1 was reasonably reached, 18.4 ± 3.5 plants m-2
D2 was 35 ± 5.7 plants m-2
D1 density constant during time
D2 density decreased from 40 p m-2 to 30 p m-2 at final harvests
No important influence of the variety on emergence on field.
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Height (cm) and Base stem diameter (mm) during time
S1plant height (cm)
S2
S1
base stem diameter (mm)
S2
0 25 50 75 100 125 150 175 200 225
DAE
cm
0
5
10
15
20
25
30mmplant height (cm)
D1
D2
D1
D2
base stem diameter (mm)
0
25
50
75
100
125
150
175
200
225
150 175 200 225 250 275 300 325 350 375
DOY
cm
0
5
10
15
20
25
30mm
Effect of sowing time Effect of density
V1plant height (cm)
V2
V1
base stem diameter (mm)
V2
150 175 200 225 250 275 300 325 350 375
DOY
cm
0
5
10
15
20
25
30mm
N0
plant height (cm)
base stem diameter (mm)
N2 N1N3
N0N1N2
N3
0
25
50
75
100
125
150
175
200
225
0 25 50 75 100 125 150 175 200 225
DAE
cm
0
5
10
15
20
25
30mm
Effect of genotype Effect of N level
234 cm by S1, D1, Tainung2
164 cm in 2003
D2 density reached the lower heights for both the sowing times.
Task 2.3 similar height for all N levels, N2: 207 cmN0: 190 cm
20 mm Ø by S1 D1 Everglades41
very similar values for all N levels: 16 mm
N0: 14 mm
Last two harvests showed a stabilization of plant height,
the base stem diameter seems to stop increasing already after 75 DAE,
similar to 2003 trends
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Fresh yields (t ha-1) of total biomass and stems during time
V1
Fresh biomass
V2
Fresh stems
V1V2
0
10
20
30
40
50
60
70
80
150 175 200 225 250 275 300 325 350 375
DOY
t ha -1
Effect of genotypeVertical lines represent standard error
Fresh biomass
N0
N1
N2Fresh stems
N3
N0N1
N2N3
0
10
20
30
40
50
60
70
80
90
0 25 50 75 100 125 150 175 200 225
DAE
t ha -1
Effect of N levelVertical lines represent standard error
Fresh biomassD1
Fresh stems
D1D2
D2
0
10
20
30
40
50
60
70
80
150 175 200 225 250 275 300 325 350 375
DOY
t ha -1Effect of density
Vertical lines represent standard error
S1Fresh biomass
S2
Fresh stems
S1
S2
0
10
20
30
40
50
60
70
80
0 25 50 75 100 125 150 175 200 225
DAE
t ha -1
Effect of sowing timeVertical lines represent standard error Best performance for N1
and for S1 Tainung2, D1 and D2
75 t ha-1 Fresh biomass(52 t ha-1 in 2003) at harvest VIII (13th October)
Sowing times affected fresh biomass yield and mainly fresh stem yield,
at the final harvest S1 reached up to 30 t ha-1 of fresh stem instead of 20 of S2.
The stem growth rate for both sowing times was similar until 125 DAE
All trends were also very similar, like 2003 year.
During the last surveys fresh yields decreased significantly, as moisture content on plant decreased too
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
S1
S2
0
10
20
30
40
50
60
20 70 120 170
DA E
t ha-1
Fresh stem (t ha-1)of S1 and S2 thesis
S1
S2
0
10
20
30
40
50
60
20 70 120 170
DA E
t ha-1
Fresh stem (t ha-1)of S1 and S2 thesis
Fresh yields (t ha-1) of total biomass and stems
Fresh biomass S1
S2
0
10
20
30
40
50
60
70
80
0 25 50 75 100 125 150 175 200 225
DAE
t ha -1
2004 Fresh biomass (t ha-1)
S1
Fresh stems
S2
0
10
20
30
40
50
60
70
80
0 25 50 75 100 125 150 175 200 225
DAE
t ha -1
Fresh stem (t ha-1)
S1
S2
0
10
20
30
40
50
60
20 70 120 170
DA E
t ha-1
Fresh biomass (t ha-1)of S1 and S2 thesis
S1
S2
0
10
20
30
40
50
60
20 70 120 170
DA E
t ha-1
Fresh biomass (t ha-1)of S1 and S2 thesis
2003
S1
S2
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Stems
S1
S1Petioles
S2
S1
Leaves
S2
S2
0
10
20
30
40
50
60
70
80
90
100
0 25 50 75 100 125 150 175 200 225
DAE
%
petioles
leaves
stems
0
10
20
30
40
50
60
70
80
90
100
0 25 50 75 100 125 150 175 200 225
DAE
%
Effect of sowing time
Fresh biomass partition in Task 2.3
Partition of FB on plant organs during time
The same consideration can be done for N level
Stem % increased during time, Leaves and petioles decreased until to reach almost 0 at the final harvests.
Sowing time seems to have a little effect on partition of biomass in plant organs.
S2 showed higher values of leaves % during time until November.
Density and genotype did not affected the partition of biomass on plant organs.
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Dry Biomass yields (t ha-1)
S1
Dry biomass
S2Dry stems
S1
S2
0
5
10
15
20
0 25 50 75 100 125 150 175 200 225
DAE
t ha -1 Effect of sowing time vertical lines represent standard error
Dry biomass
D1Dry stems
D1
D2
D2
0
5
10
15
20
150 175 200 225 250 275 300 325 350 375
DOY
t ha -1 Effect of densityvertical lines represent standard error
V1Dry biomass
V2
Dry stems
V1
V2
0
5
10
15
20
150 175 200 225 250 275 300 325 350 375
DOY
t ha -1Effect of genotype
vertical lines represent standard error
Dry biomass
N0
N1
N2
Dry stems
N3
N0
N1
N2N3
0
5
10
15
20
0 25 50 75 100 125 150 175 200 225
DAE
t ha -1
Effect of N level vertical lines represent standard error
Dry biomass increased significantly until October
18 t ha-1 at 155 DAE (11 t ha-1 at 140 DAE in 2003)by S1 D1 Tainung2
Trends of all treatments were very similar.
S1 produced more than S2, as D2 and Tainung2 produced more than D1 and Everglades41
…differences was not so relevant and the growth rates were comparable
At 150 DAE N1 and N3 treatment showed the best performance (up to 18 t ha-1)…high standard error
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Dry stem yields (t ha-1)
S1
Dry biomass
S2Dry stems
S1
S2
0
5
10
15
20
0 25 50 75 100 125 150 175 200 225
DAE
t ha -1 Effect of sowing time vertical lines represent standard error
Dry biomass
D1Dry stems
D1
D2
D2
0
5
10
15
20
150 175 200 225 250 275 300 325 350 375
DOY
t ha -1 Effect of densityvertical lines represent standard error
V1Dry biomass
V2
Dry stems
V1
V2
0
5
10
15
20
150 175 200 225 250 275 300 325 350 375
DOY
t ha -1Effect of genotype
vertical lines represent standard error
Dry biomass
N0
N1
N2
Dry stems
N3
N0
N1
N2N3
0
5
10
15
20
0 25 50 75 100 125 150 175 200 225
DAE
t ha -1
Effect of N level vertical lines represent standard error
Trends were quite similar
Best performance was shown by combination S1 D1 Tainung2 (13 t ha-1)
Plants of S1 treatments increased dry stem biomass until around 190 DAE, while S2 (sowed 30 days after S1) stopped to growth around 125 DAE.
This resulted in a quite higher yields of S1 treatments respect to S2
At the last survey in all the cases dry stem yield slightly decreased.
N0 higher values (up to 11 t ha-1)…high standard error
…3 t ha-1 of dry leaves can be estimated for N1 and N3 treatment at harvest VIII
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
S1
Dry stems
S2
Dry biomassS1
S2
0
2
4
6
8
10
12
14
16
18
20
0 500 1000 1500 2000
acc GDD
t ha-1
Effect of sowing time on dry biomass yield and stems respect to the accumulation of the GDD (°C)
Dry stem yields (t ha-1)
In all cases S1 is up to S2
S2 needed more growing degree days to reach same values of S1 yield
S1
S2
0
2
4
6
8
10
12
14
20 70 120 170
DAE
t ha-12003
S1
S2
0
2
4
6
8
10
12
14
20 70 120 170 220
DAE
t ha-1 2004The higher value reached by
S1 in 2003 at 170 DAE (5th November) was reached by S1 in 2004 at 120 DAE (8th September),
…even if difference in sowing time was only of 10 days
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Dry biomass content (%) during time
% dry content in plant
0
10
20
30
40
50
150 175 200 225 250 275 300 325 350 375
DOY
% % dry content in plant
0
10
20
30
40
50
150 175 200 225 250 275 300 325 350 375
DOY
%
Dry biomass content (%) in plant during time in Task 2.2vertical lines represent standard error
Dry biomass content (%) in plant during time in Task 2.3vertical lines represent standard error
Dry matter content increased during time (from 10 to 40 %) a big jump was observed at the final harvest carried out on the middle of December
For all treatments trends were very similar
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Leaf Area Index and Specific Leaf Area during time
S1
LAI
SLA
S2
S2
S1
0
5
10
15
0 25 50 75 100 125 150 175 200 225
DAE
LAI (m 2 m -2)
0
4
8
12
16
20
24SLA
(m2 kg-1)
Effect of sowing timevertical lines represent standard error
LAI
SLA
D1
D1
D2
D2
0
5
10
15
150 175 200 225 250 275 300 325 350 375
DOY
LAI (m 2 m -2)
0
4
8
12
16
20
24SLA
(m2 kg-1)
Effect of densityvertical lines represent standard error
V1
V2LAI
V2V1 SLA
0
5
10
15
150 175 200 225 250 275 300 325 350 375
DOY
LAI (m 2 m -2)
0
4
8
12
16
20
24SLA
(m2 kg-1)
Effect of genotypevertical lines represent standard error
N0
SLAN0
N1
N1
LAIN2
N2
N3
N3
0
5
10
15
0 25 50 75 100 125 150 175 200 225
DAE
LAI (m 2 m -2)
0
4
8
12
16
20
24SLA
(m2 kg-1)
Effect of N levelvertical lines represent standard error
Sowing time did not affected LAI as final value…even though during the crop cycle S2 trend were usually up to S1 curve
D2 and Tainung2 showed higher values of LAI respect to D1 and Everglades41,… differences were
not relevant
N3 treatment seems to produce more leaves per m-2 during time, but again difference was not so important to consider it.
In terms of SLA, the most relevant difference was showed by D2 density, but in any case trends were very similar.
RESULTS
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Final harvests (5 m2)
At harvest soil moisture was in a range of 40 % to 50 % (V/V)
Crop did not reached complete flowering
Plants lost leaves before the harvests, all yields are referred to stem
Analysis of variance (ANOVA) for all treatments and their interaction:
Task 2.2Task 2.2
4 factors complete randomized block design
• Variety • Density • Sowing time• Harvest(2 levels)
Task 2.3Task 2.3
2 factors complete randomized block design
• N (4 levels)• Harvest (2 levels)
Differences analyzed at P = 0.05 and 0.01
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Reached density on field was significantly different between the two D thesis.
In both the last harvests n° of plants m-2 was significantly higher for the S1 treatments than S2 ones (P = 0.01)
Trends of some interesting parameters related to the factor that significantly affect their value
0
5
10
15
20
25
30
S1 S2 S1 S2
20 40
Task 2.2
Plants (n° m-2)
Similar to 2003.In plants of S1 and D1 were present more nodes, around 40
Sowing time affected significantly the n° of nodes, S1 10 nodes more than S2.
Everglades41 more nodes than Tainung2 (P=0.05) …little statistical significance also among replications
n° of nodes
05
1015202530354045
S1 S2 S1 S2
Everglades41 Tainung2
Task 2.2
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Height and base stem diameter were influenced mainly by tested densities and sowing times, …as it was observed in 2003.
D1 and S1 treatments reached the higher values.
0
50
150
200
S1 S2 S1 S2
100
20 40
Task 2.2
Plant height (cm)
0
5
10
15
20
S1 S2 S1 S2
20 40
Task 2.2
Base stem diameter (mm)
Trends of some interesting parameters related to the factor that significantly affect their value
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Fresh yields of stems, bark and core were influenced significantly by sowing and harvest time,
The relevant difference between the harvest A and B was mainly due to the lost of moisture, as confirmed by the statistically significant increasing of dry content in stem, bark and core at harvest B respect to harvest A.
Fresh stem yield ranged from up to 55 t ha-1 to 20 t ha-1.
Higher values were observed at harvest A by all first sowing time treatments, 55 against 40 t ha-1 of S2 treatments.
Trends of some interesting parameters related to the factor that significantly affect their value
Fresh stem yieldt/ha
Fresh core yield t/ha
Fresh bark yield t/ha
0
10
20
30
40
50
60
S1 S2 S1 S2
A B
Task 2.2
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Trends of some interesting parameters related to the factor that significantly affect their value
Dry stem yield was influenced mainly by sowing time and density, while harvest time seemed do not affect it.
D1 and S1 treatments reached the highest values, around 13 t ha-1, against 8 t ha-1 of 2003 year.
Also variety and harvest time seems to influence dry stem yield, with a higher production of Tainung2 respect to Everglades41 at harvest A (P=0.05)… little statistical significance also among replications.
Dry bark and core yields are also influenced by sowing time but not by harvest time and density.
Dry core yield ranged from 6.5 to 10 t ha-1, while dry bark reached at maximum 5.8 t ha-1, higher values were reached in all cases again by S1 treatments.
02468
1012
S1 S2 S1 S2
20 40
Task 2.2
Dry stem yield (t ha-1)
0
2
4
6
8
10
12
S1 S2
Task 2.2
Dry bark yield t ha-1
Dry core yield t ha-1
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Trends of some interesting parameters related to the factor that significantly affect their value
A considerable number of plants, from up to 50 % to 95 % of harvested plants, has a branched stem, with at least 2 important ramifications per plant.
Density influenced significantly the presence of the branches, with higher values of branched plants and n° of ramifications per plants reached by D1 density.
S2 plants present more branched stems.
0102030405060708090
20 40 20 40
S1 S2
Task 2.2
Branched plants (% of harvested plants)
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
Trends of some interesting parameters related to the factor that significantly affect their value
N2: the highest plant height (P=0.05).
The statistical analysis showed that only the harvest factor seems to affect fresh yields, and dry content
… dry yields were not significantly affected by harvest
180
185
190
195
200
205
N0 N1 N2 N3
Task 2.3
Plant height (cm)
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
RESULTSN and C content (%) in leaves, stems and soil at final harvest of Task 2.3
N content in stem and leaf seems to increase with N level until N2
… differences are not relevant, as confirmed by the analysis of the variance
Carbon content in leaf and stems seems also to increase with N level, reaching values up to 43% in leaf of N3 treatment
…differences among treatments are significant at P=0.05
0,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
leaf stem soil leaf stem soil leaf stem soil leaf stem soil
N0 N1 N2 N3
41,0
41,5
42,0
42,5
43,0
leaf stem soil leaf stem soil leaf stem soil leaf stem soil
N0 N1 N2 N3
Nitrogen content (%)
Carbon content (%)
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
RESULTS
QE:QE:apparent quantum efficiencyapparent quantum efficiency
Light compensation pointLight compensation point
Amax:Amax:light saturated maximumlight saturated maximum
K: K: convexityconvexity
Light compensation point = 209
Amax=35.1QE=0.046K=0.605
Saturated PPFD=828
Amax=36.2QE=0.058K=0.274
Light compensation point = 169
Saturated PPFD=784
First First sowingsowing timetime
SecondSecond sowingsowing timetime
S2 higher QE, AmaxS2 higher QE, Amax
S2 lower light S2 lower light compensation pointcompensation point
leaves of S2 more effective leaves of S2 more effective in photosynthesisin photosynthesis
Portable IRGA (CIRASPortable IRGA (CIRAS--2, PP2, PP--Systems)Systems)
Tainung2; D 40 p mTainung2; D 40 p m--22
Most recently fully expanded leavesMost recently fully expanded leaves
The light response curve of leaf photosynthesis (A)
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
RESULTS Ash content in core (%)
0,0
1,0
2,0
3,0
4,0
5,0
6,0
7,0
Harvest A Harvest B Harvest A Harvest B
Task 2.2 Task 2.3
Media di Hash (%)
Ash content in Kenaf core is on average around 5.5 %, like more commonly utilized herbaceous plant for energy production
The differences among factors seem to be not important (analysis of the variance)
The harvest time: harvest B showed higher values of ash % for all tested factors, increasing of more than 1 % the hash content, observed only in Task 2.2.
Ash content in core (%)
Harvest A Harvest B Harves t A Harves t B
Everglades41 5,219 6,439 0,708 0,815
Tainung2 4,848 6,121 0,495 0,822
S1 5,176 6,414 0,518 0,874
S2 4,890 6,146 0,712 0,770
D1 5,174 6,156 0,696 0,841
D2 4,893 6,405 0,541 0,809
s tandard deviation
Task 2.2
ash %
N level Harvest A Harvest B Harvest A Harvest BN0 5,574 5,672 0,680 0,061
N1 5,347 6,169 0,366 0,201
N2 5,322 5,913 0,444 0,745
N3 5,637 5,667 0,173 1,063
Task 2.3
ash % standard deviation
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
RESULTS
In all the cases fresh weight increased significantly with distance from the star centre.
Also bark yields increased, even if bark % respect to the stem seemed to decrease
Both stem height and base diameter increased going far away from the centre of the star (increasing distance between the row)
Height seems to have a stabilization at the end, where in any case it is possible to observe a big variation.
Lower value of R2 anyway showed a bad estimation of the tendency curve, related to the high variation of the measurement recorded.
A possible reason could be the damages that hail made on plants of this trial, particularly seriously in this trial
Effect of the distance between the rows on plant biometric parameters and yields
height (cm )
base stem diam eter (m m )
R 2 = 0,8465
R 2 = 0 ,7139
0
50
100
150
200
250
0 50 100 150 200 250 300 distance(cm )
(cm
)
0
5
10
15
20
25
30
35
40
45
50
(mm
)
fresh weight (g)
fresh bark (g)
dry bark (g)
% of fresh bark
dry matter in bark (%)
0
200
400
600
800
1000
1200
1400
20 50 150 250 350distance from centre (cm)
(g)
0
5
10
15
20
25
30
35
40
45
50
(%)
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
CONCLUSIONS
year 2004 more productively than 2003
Fresh and dry yields, stem height and LAI reached values significantly higher than 2003
2004 2003 2004 2003Height (cm) 234 164 S1 D1 V1 S1 D1 V1
Fresh Yield (t ha -1) 75 52 S1 D1 V1 S1 D2 V1Dry Yield (t ha -1) 18 11 S1 D1 V1 S1 D1 V2
Dry stem yield (t ha -1) 13 9 S1 D1 V1 S1 D1 V1LAI (m 2 m -2) 10 6 S2 D2 V2 S1 D2 V2
Year Year
The higher amount of rainfall, mainly in May and June, respect to 2003
Not particular dry season
Not particular cold temperatures were recorded, at the end of the crop cycle
…plants accumulated more growing degree days respect to the first year
Better climatic conditions:
Max reached values of some interesting parameters in 2004 and 2003
it has to be considered that yield levels of 2003 were very low, as observed for the other crops in the same location
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
CONCLUSIONS
Sowing time affected most of investigated parameters, but the difference was lower than what observed during the last year
Increasing crop cycle length…Sowing at the beginning of May increased in 2004 the length of the crop cycle respect to 2003, without any kind of
problems for the plant growth
Two tested varieties did not show important differences as well as nitrogen levels seem not to influence yields, even if in N1 and N3 levels have been observed significantly higher values of LAI
The effects of density were low for the yield parameter, influencing mainly stem diameter and plant height
Harvest time mainly affected the fresh yields, significantly decreasing the moisture content in the last harvest
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
KENAF FIBRE
Tainung2
First sowing time
Plant height: 87 cm
Base stem Ø: 11.38 mm
10 X10 XThanks to Federica Pelatti
20 X20 X
10 cm from stem bottom
BioKenaf Project – 5th Technical Meeting Catania – July 5 - 6, 2005www.agrsci.unibo.it/dista/grici/
SowingSowing
2005 EXPERIMENTAL FIELDS
Task 2.2 S1: 28 04 2005
S2: 30 05 2005
Task 2.3: 10 05 2005
Task 2.2
S2V2 D1
S1V1 D2
S1V2 D2
S2V1 D1
S2V1 D2
S2V2 D2
S1V1 D1
S1V2 D1
S2V2 D2
S2V1 D2
S1V1 D1
S1V1 D2
S1V2 D1
S2V2 D1
S1V2 D2
S2V1 D1
S1V2 D2
S1V2 D1
S2V2 D1
S2V2 D2
S2V1 D1
S1V1 D1
S2V1 D2
S1V1 D2
N1
N3
N0
N2
38 m
4.5 7 4 47
4
7 4.5
48
102 m
Block 1 Block 3
S2V1 D1
S1V2 D1
S1V1 D1
S2V2 D2
S2V2 D1
S1V1 D2
S2V1 D2
N2
N0
N3
N1
N2
N1
N3
N0
N0
N3
N2
N1
7 m
3
6 m
6 m
14 m
24
Block 4
Block 3
Block 2
Block 1
110 m
2 m
24
5 m
S1V2 D2
Block 4
Task 2.2
S2V2 D1
S1V1 D2
S1V2 D2
S2V1 D1
S2V1 D2
S2V2 D2
S1V1 D1
S1V2 D1
S2V2 D2
S2V1 D2
S1V1 D1
S1V1 D2
S1V2 D1
S2V2 D1
S1V2 D2
S2V1 D1
S1V2 D2
S1V2 D1
S2V2 D1
S2V2 D2
S2V1 D1
S1V1 D1
S2V1 D2
S1V1 D2
N1
N3
N0
N2
38 m
4.5 7 4 47
4
7 4.5
48
102 m
Block 1 Block 3
S2V1 D1
S1V2 D1
S1V1 D1
S2V2 D2
S2V2 D1
S1V1 D2
S2V1 D2
N2
N0
N3
N1
N2
N1
N3
N0
N0
N3
N2
N1
7 m
3
6 m
6 m
14 m
24
Block 4
Block 3
Block 2
Block 1
110 m
2 m
24
5 m
S1V2 D2
Block 4
S2V2 D1
S1V1 D2
S1V2 D2
S2V1 D1
S2V1 D2
S2V2 D2
S1V1 D1
S1V2 D1
S2V2 D2
S2V1 D2
S1V1 D1
S1V1 D2
S1V2 D1
S2V2 D1
S1V2 D2
S2V1 D1
S1V2 D2
S1V2 D1
S2V2 D1
S2V2 D2
S2V1 D1
S1V1 D1
S2V1 D2
S1V1 D2
N1
N3
N0
N2
N1
N3
N0
N2
38 m
4.5 7 4 47
4
7 4.5
48
102 m
Block 1 Block 3
S2V1 D1
S1V2 D1
S1V1 D1
S2V2 D2
S2V2 D1
S1V1 D2
S2V1 D2
N2
N0
N3
N1
N2
N0
N3
N1
N2
N1
N3
N0
N2
N1
N3
N0
N0
N3
N2
N1
N0
N3
N2
N1
7 m
3
6 m
6 m
14 m
24
Block 4
Block 3
Block 2
Block 1
110 m
2 m
24
5 m
S1V2 D2
Block 4
HarvestsHarvests21 06 2005
28 06 2005