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2010 Annual Report
2 ISU Veterinary Diagnostic Laboratory
Table of Contents
ISU VDL Mission Statement ...................................................................................................................... 3
VDPAM Organizational Chart .................................................................................................................... 4
VDPAM External Advisory Board .............................................................................................................. 5
VDL Faculty List ........................................................................................................................................ 6
New VDL Faculty in 2012 .......................................................................................................................... 6
VDL Staff List ............................................................................................................................................ 7
Honors and Awards ................................................................................................................................... 9
New Tests Introduced in 2012 ................................................................................................................. 10
CYCADS - New VDL Section Formed ..................................................................................................... 11
2012 Statistical Data ............................................................................................................................... 12
Accessions ......................................................................................................................................... 12
Tests ................................................................................................................................................... 13
Submission Trends ............................................................................................................................. 13
Species Submission Data ................................................................................................................... 14
Surveillance Testing............................................................................................................................ 16
Bacteriology Testing ........................................................................................................................... 17
Antimicrobial Susceptibility Profiles ............................................................................................ 21
CYCADS Testing ................................................................................................................................ 31
Molecular and Health Assurance (HATS) Testing ............................................................................... 31
Pathology Testing ............................................................................................................................... 34
Serology Testing ................................................................................................................................. 34
Toxicology/Nutrition Testing ................................................................................................................ 37
Virology Testing .................................................................................................................................. 42
Rabies ........................................................................................................................................ 44
Glossary ................................................................................................................................................. 46
The Iowa State University Veterinary Diagnostic Laboratory (VDL)
is accredited as a full service laboratory by the American Association of Veterinary Laboratory Diagnosticians (AAVLD)
"Iowa State University does not discriminate on the basis of race, color, age, religion, national origin, sexual orientation, gender identity, genetic information, sex, marital status, disability, or status as a U.S. veteran. Inquiries can be directed to the Director of Equal Opportunity and Compliance, 3280 Beardshear Hall, (515) 294-7612."
3 ISU Veterinary Diagnostic Laboratory
ISU VDL Mission Statement
It is the mission of the Iowa State University Veterinary Diagnostic Laboratory to provide comprehensive and cutting edge diagnostic services to veterinarians, producers, and animal owners in Iowa and nationally. The lab is responsible for delivering accessible, timely, accurate, valid, and consistent test results to aid in the protection of animal and human health. Other services include a wide range of surveillance testing for early detection and identification of foreign animal and emerging domestic disease agents, as well as acts of bioterrorism directed at human and livestock populations and agricultural food supplies. The VDL also provides educational opportunities to professional and graduate students, as well as local, national and international scientists, diagnosticians and practitioners. Research is an important component of the tripartite mission as faculty and staff develop state-of-the-art diagnostic tools and techniques and also direct studies which provide new insights and deeper understanding of pathogenesis, transmission, and immunomodulation of infectious diseases.
Our Tripartite Mission
• Provide comprehensive and cutting edge diagnostic services • Deliver accessible, timely, accurate, valid and consistent test results • Provide educational opportunities to professional and graduate students, scientists, diagnosticians
and practitioners
4 ISU Veterinary Diagnostic Laboratory
VDPAM Organizational Chart
5 ISU Veterinary Diagnostic Laboratory
VDPAM External Advisory Board
Name Title/Position Company/Clinic City
Dr. Pat Halbur Chair of VDPAM, Executive Director of the ISU VDL, Co-chair of VDPAM Advisory Board
ISU College of Vet Med, VDPAM
Ames, IA
Dr. Jodie Pettit Private Practitioner with swine, beef, equine and dairy focus. Co-chair of VDPAM Advisory Board, President-Elect IVMA
Audubon-Manning Veterinary Clinic
Audubon, IA
Dr. Rodger Main Director of Operations, ISU VDL ISU College of Vet Med, (VDL)
Ames, IA
Dr. Pat Gorden Director of Food Supply Veterinary Medicine ISU College of Vet Med, VDPAM (FSVM)
Ames, IA
Dr. Mark Brinkman Private Practitioner with swine, beef, and small animal focus
Veterinary Medical Center
Williamsburg, IA
Christian Davies Accountant and Alpaca Producer C&M Acres Maxwell, IA
Dr. Ann Garvey State Public Health Veterinarian Iowa Dept of Public Health
Des Moines, IA
Dr. John Hicks Private Practitioner with swine and beef focus Carroll Veterinary Clinic Carroll, IA
Dr. Marlin Hoogland Veterinarian, Murphy Brown Western Operations, integrated food supply company
Murphy Brown Orange City, IA
Dr. Gavin Meerdink Toxicologist, former VDL Director University of Illinois Mahoment, IL
Dr. Lisa Nolan Dean of Veterinary Medicine ISU College of Vet Med Ames, IA
Bill Northey Iowa Secretary of Agriculture Department of Agriculture & Land Stewardship
Des Moines, IA
Dr. Jodie Pettit Private Practitioner with swine, beef, equine and dairy focus. Co-chair of VDPAM Advisory Board, President-Elect IVMA
Audubon-Manning Veterinary Clinic
Audubon, IA
Dr. Dave Schmitt/ Dr. Randy Wheeler
State Veterinarian for Iowa - Dr. Schmitt Assistant State Veterinarian - Wheeler
Iowa Department of Agriculture and Land Stewardship
Des Moines, IA
Dr. Don Sievers Practitioner Slater Animal Hospital Slater, IA
Dr. Gene Tinker AFO Coordinator Iowa Dept of Natural Resources
Manchester, IA
Dr. Mahlon "Whitey" Vorhies
Former Director SDSU and KSU VDL, former Department Chair at KSU
Amana, IA
6 ISU Veterinary Diagnostic Laboratory
VDL Faculty List
Halbur, Pat Chair of VDPAM and
Executive Director of ISU VDL Administration, Circovirus, Hepatitis E Virus, Swine Respiratory Disease, PRRSV
Main, Rodger Director of Operations Swine Production Medicine and Nutrition [email protected]
Arruda, Paulo Adjunct Instructor Pathology [email protected]
Burrough, Eric Assistant Professor Pathology, Brachyspira, Campylobacter [email protected]
Coetzee, Hans Associate Professor Cyclone Analytical Detection Service [email protected]
Cooper, V.L. Senior Clinician, Pathologist BVD, Beef Cattle, Dairy, Caprine, Equine, Canine, Feline
Ensley, Steve
Senior Clinician, Toxicology Section Leader
Toxicology, Field Investigation of Tox Issues, Drinking Water Quality, Corn co-products
Frana, Tim Associate Professor Bacteriology Section Leader
Bacterial Diseases, Epidemiology, Diagnostics
Gauger, Phil Assistant Professor Pathology Molecular Section Leader
Pathology, Virology, Swine Influenza, PRRSV, PCV2
Harmon, Karen Adjunct Assistant Professor, Molecular Microbiology, HATS Section Leader
Molecular Testing and Characterization of Animal Bacteria and Viruses
Imerman, Paula Clinician Toxicology/Nutrition [email protected]
Janke, Bruce Professor, Pathology Swine Influenza, Enteric Diseases of Pigs, Pathology
Johnson, John Clinician and Serology Section Leader
Development of Serology Tests and Quality Assurance
Kinyon, Joann Adjunct Assistant Professor, Bacteriology/Clinical Microbiology Section Leader
Bacteriology/Clinical Microbiology [email protected]
Madson, Darin Assistant Professor, Pathology Swine Disease Diagnosis, Nursery/Grow-Finish Health
Opriessnig, Tanja Professor, Pathologist Porcine Circovirus, Erysipelas, Hepatitis E virus, Porcine Parvovirus, Swine Respiratory Diseases
Rumbeiha, Wilson Professor Toxicology/Nutrition [email protected] Schwartz, Kent Senior Clinician, Pathology
Section Leader Pathology, Swine and Beef Diagnostics, Evidence-Based Practice
Snider, Doug Veterinary Diagnostician Toxicology [email protected]
Stevenson, Greg Professor Pathology, Swine Diseases, Diag. Medicine in Food Producing Animals
Yoon Kyoung-Jin Professor Viral Diseases, PRRSV, Influenza, PCV2, Development of Diagnostic Tools
Zhang, Jianqiang Assistant Professor Molecular & viral diagnostics [email protected] Zimmerman, Jeffrey Professor, Epidemiologist Ecology of Viral Infections of Swine [email protected]
New VDL Faculty in 2012
Borys , Moira Adjunct Instructor Microbiology [email protected]
Haas, Nathaniel Adjunct Instructor Microbiology [email protected]
Li, Ganwu Research Assistant Professor Microbiology [email protected]
Pillatzki, Angela Veterinary Diagnostician Pathology [email protected]
7 ISU Veterinary Diagnostic Laboratory
VDL Staff List
Name Degrees Position Date Hired
Abate, Sarah BS Research Associate II 2008
Adams, Carolyn Clerk III 1994
Aust, Ronisue Histo Technician I 2011
Bade, Daniel BS Research Associate I 2011
Barnes, Kimberly BS Research Associate I 2012
Berghefer, Randy AA, BS Manager Information Technology I 1997
Block, Suzanne BS Laboratory Technician III 2002
Boesenberg, Kelly BS, MS Program Manager I 2006
Boor, Jessica BS Quality Assurance Officer 2006
Bower, Leslie BS, MS Research Associate II 2009
Bradner, Laura BS Research Associate I 2013
Branaman, Rosalyn BS Histology Technician II 1988
Brodie, Joseph AA, BLS Assistant Scientist I 2007
Carter, Sylvia BS Laboratory Technician III 1994
Chriswell, Amy BS Assistant Scientist I 1999
Clark, Cheryl Laboratory Technician III 2011
Clauson, Susan Clerk III 1993
Cramer, Susan BS, MS Research Associate I 2011
Crim, Bret BA Systems Analyst I 2012
Cullen, Johnah BS Research Associate I 2012
Deike, Paige BS Research Associate I 2012
Dunn, Derek BS Research Associate I 2011
Dunn, Linda Clerk III 2000
Emge, Patrick BS Laboratory Technician II 2002
Endreland, Luanne Laboratory Technician II 1996
Fenneman, Dawn BS Histo Technician II 2007
Finch, Mary Clerk III 2006
Fligg, Danielle BS Research Associate I 2011
Frisk, David Clerk II 2006
Fulster, Susan BS Laboratory Technician II 1988
Gard, Theresa BS Research Associate I 2007
Gregory, Marissa BS Research Associate II 2008
Grover, Raymond Jr. BA Laboratory Technician III 1997
Gudenkauf, Kylie BS Research Associate I 2015
Hadaway, Hannah Lab Assistant II 2012
Hall, Timothy BS System Support Specialist II 2007
Haney, Devon BS Research Associate II 2007
Heinen, Sheila BS Laboratory Technician III 2001
Heyer, Jessica Lab Assistant II 2012
Holdredge, Jennifer BS Administrative Specialist II 1989
Huffman, Elise BA Lab Technician III 2012
Ivanytska, Olha Research Associate I 2007
Jones, Jenny Clerk III 2012
8 ISU Veterinary Diagnostic Laboratory
Kalkwarf, Erin BS Research Associate II 2006
Klein, Brianna Research Associate I 2012
Koeneke, Stacy Clerk II 2011
Kostohryz, Scott BS Research Associate I 2006
Kowalke, Kary Lab Assistant II 2012
Kurth, Lacey BS Research Associate I 2012
Lee, Christine Account Specialist 1996
Lin, Kathy BS Assistant Scientist I 2002
Lueth, Silvano Lab Assistant II 2012
Magtoto, Ronaldo BS, MS, PhD Research Associate I 2007
Matias Ferreyra, Franco DVM Research Associate I 2012
McCammant, Capri Histology Technician II 2005
McDeid, Leona AA, BA Assistant Scientist I 1997
Meraz, Christine Clerk IV 1994
Mindham, Steven BS Systems Support Specialist III 2004
Minor, Ashley BS Research Associate I 2011
Nelson, Nancy AA, BS Laboratory Technician II 1995
Norris, Sheila BS Assistant Scientist II 1992
Novak, Renee AA Program Assistant II 1990
Olson, Linda Clerk III 1995
Peterson, Jacqueline BA Research Associate II 1988
Raymond, Matt BS Research Associate I 2010
Schrunk, Dwayne BS Research Associate II 1997
Schubert, Abby BS Research Associate I 2012
Schuller, Bruce Facilities Coordinator 2005
Schultz, Daniel BS Laboratory Technician III 2001
Schulz, Leanne BS Research Associate I 2011
Schwarz, Brenda BS Laboratory Assistant I 1990
Sickau, Diane BS Research Associate II 1987
Smith, Linda BA Histology Technician I 2007
Sreedharakurup, Premchand BS Systems Analyst II 2011
Stensland, Wendy BS Assistant Scientist I 2001
Thimmesch, Carrie Lab Technician II 2012
Thompson, Curt BS, MBA Assistant Scientist II 2006
Tuttle, Vanna Clerk III 1996
Upchurch, Nancy Laboratory Technician II 2008
Warneke, Hallie BA Research Associate I 2011
Weeda, Matthew BS Research Associate I 2010
Wilson, Kristine Clerk III 2006
Wulf, Larry BS, MS, PhD Assistant Scientist III 2006
Zagar, Rose BS Clerk III 1990
9 ISU Veterinary Diagnostic Laboratory
Honors and Awards
Baker, Rodney 2012 Alumni of the Year – Western Kentucky University, Odgen College of Science and Technology Burrough, Eric Boehringer Ingelheim Vetmedica Professorship in Food Animal Infectious Diseases Main, Rodger Alpha Gamma Rho (Iowa State University) Alumni Achievement Award, 2012 Recognizing professional contributions & achievement in agriculture. Yoon, Kyoungjin Taishan Scholar Oversea Distinguished Expert, recommended by Institute of Animal Science and Veterinary Medicine at Shandong Academy of Agricultural Sciences. Pending approval from the Shandong Ministry of Education, China Service Team of the Year Award, College of Veterinary Medicine, Iowa State University Zimmerman, Jeffrey Appointed to National Pork Board Swine Health Committee
10 ISU Veterinary Diagnostic Laboratory
New Tests Introduced in 2012 Test name Test department
Decal Histopathology
Immunohistochemistry B-cell, CD79 (VETPATH) Histopathology
Immunohistochemistry Canine CD18 (VETPATH) Histopathology
Immunohistochemistry C-kit (CD117) (VETPATH) Histopathology
Immunohistochemistry Factor VIII (VETPATH) Histopathology
Immunohistochemistry Kappa/Lambda (VETPATH) Histopathology
Immunohistochemistry Melan A (VETPATH) Histopathology
Immunohistochemistry S-100 (VETPATH) Histopathology
Immunohistochemistry T-Cell, CD3 (VETPATH) Histopathology
Immunohistochemistry Vimentin (VETPATH) Histopathology
Influenza A general - PCR Molecular Diagnostics
Influenza A subtype- PCR Molecular Diagnostics
PCR - Applied Biosystems M. hyopneumoniae Molecular Diagnostics
PCR - Bovine Respiratory Bacterial Panel Molecular Diagnostics
PCR - Bovine Respiratory Viral and Bacterial Panel Molecular Diagnostics
PCR - Bovine Respiratory Viral Panel Molecular Diagnostics
PCR - Influenza A - NAHLN surveillance Molecular Diagnostics
PCR - Influenza A Quantitation Molecular Diagnostics
PCR - Influenza A surv subtype Molecular Diagnostics
PCR - Influenza HA/NA gene subtyping Molecular Diagnostics
PCR - Influenza type A NP gene Molecular Diagnostics
PCR - M. flocculare Molecular Diagnostics
PCR - Mycoplasma hyopneumoniae Quantitation Molecular Diagnostics
PCR - TGE Molecular Diagnostics
PCR - TGE and PRCV Molecular Diagnostics
Sequencing and Analysis - Influenza NAHLN surv Molecular Diagnostics
Sequencing and Analysis - PRRSV Molecular Diagnostics
Sequencing and Analysis - PRRSV HI-VOL Molecular Diagnostics
Quantitative Flotation - Modified Wisconsin Parasitology
BVD antigen ELISA (PI) Large EN Serology
BVD antigen ELISA (PI) Serum/EN Serology
SIV Pfizer H1N2 delta 1 Serology
Aflatoxins LC/MS/MS Screen Toxicology
Extraction: Panel Toxicology
Extraction: Single Compound Toxicology
Heavy Metals Panel Feed/Tissue Toxicology
Heavy Metals Panel for Fluid Toxicology
Hormone Free Feed Panel Toxicology
Molybdenum in fluids Toxicology
Selenium GLP Method Toxicology
Vitamin A in serum Toxicology
Vitamin E in serum Toxicology
Vitamin E in Tissue Toxicology
Virus isolation-Swine influenza NAHLN surveillance Virology
11 ISU Veterinary Diagnostic Laboratory
CYCADS - New VDL Section Formed CYCADS - Cyclone Custom Analyte Detection Service The CYCADS section of the Veterinary Diagnostic Laboratory at Iowa State University is composed of a board-certified clinical pharmacologist and toxicologists supported by analytical chemists. The group is centered around a laboratory facility with a focus on high throughput analyses intended to quantify exogenous and endogenous compounds (e.g. drugs, toxins and biomarkers) in organic matrices. It provides analytical services in support of multiple sample, known analyte, pharmacological and toxicological research for academic, industry, and government entities. CYCADS has rigorous and robust procedures in place to deal with regulatory scrutiny and has a vast array of developed and validated analytical methods focused on pharmacology.
• 80 existing extraction methods • 250 analyte detection methods • 150 validated immunoassays
Points of Pride 1. The first unit within a Veterinary Diagnostic Laboratory to offer confirmatory drug residue testing
for practitioners. 2. The first unit within a Veterinary Diagnostic Laboratory to offer analytical support for
pharmacology researchers. 3. The first lab to do anabolic steroid testing in horses in the USA. 4. The only US lab that offers steroid profiling by GC/MS. 5. The lab has an assay that can screen for 300 + compounds in a single run. 6. The only lab with a resident board-certified veterinary clinical pharmacologist on staff. 7. Five staff members each with over 20 years racing lab experience. 8. The lab is large enough to offer complete testing capabilities, small enough to offer individualized
customer service to the client.
Dr. Hans Coetzee Dr. Coetzee serves as Section, where he oversees operation of the Racing Chemistry laboratory, conducts pharmacokinetic services and research, and will initiate new services such as antimicrobial residue testing. Dr. Coetzee also teaches clinical pharmacology related topics in the veterinary and graduate curriculum and collaborates on animal welfare-related research and outreach activities in FSVM. Dr. Coetzee earned his veterinary degree from the University of Pretoria, South Africa. After practicing veterinary medicine for several years in Northern Ireland. Dr. Coetzee worked in the private pharmaceutical industry for 2 years before coming to Iowa State University to pursue a PhD in Veterinary Microbiology. He completed his PhD in 2005 and became board certified by the American College of Veterinary Clinical Pharmacology in 2006. Since 2005, Dr. Coetzee has been on the faculty of the College of Veterinary Medicine at Kansas State University (KSU) where he was promoted to Associate Professor and granted tenure in 2010. At KSU, Dr. Coetzee directed a research program focused on discovery of biomarkers of pain and distress and the development of novel pain treatment regimens. Over the past 3 years, Dr. Coetzee has received a substantial amount of competitive funding for his research from the USDA NRI Animal Well Being program and from the pharmaceutical industry. Dr. Coetzee has also demonstrated entrepreneurial success by establishing Pharmacological Clinical and AnalyTical Services Laboratory (PharmCATS) at KSU. This innovative service unit performs contract pharmacological and toxicological studies and provides assay development. Dr. Coetzee has authored or co-authored over 40 peer-reviewed manuscripts and 2 book chapters in the area of clinical pharmacology,food animal production medicine and welfare.
For more information on CYCADS Services, contact Dr. Coetzee at 515-294-7424, or e-mail address: [email protected]. Or see their website at http://vetmed.iastate.edu/diagnostic-lab/cycads
12 ISU Veterinary Diagnostic Laboratory
2012 Statistical Data Accessions 2012 Number of Accessions
Total 52,487
January 4,171
February 4,268
March 4,771
April 4,514
May 4,642
June 4,292
July 3,878
August 4,502
September 3,856
October 4,948
November 4,739
December 3,906 2012 Accessions Bacteriology CYCADS
Molecular/ HATS Pathology Serology Toxicology Virology
Total 15,102 64 30,539 12,038 17,142 1,816 2,901
January 1,228 1 2,446 1,023 1,373 146 213
February 1,323 1 2,478 1,081 1,382 160 207
March 1,347 3 2,663 1,043 1,631 230 277
April 1,341 1 2,569 1,026 1,525 163 252
May 1,344 0 2,546 1,051 1,603 141 291
June 1,280 13 2,428 946 1,468 111 197
July 990 7 2,193 740 1,298 124 242
August 1,151 9 2,540 919 1,408 169 273
September 1,049 8 2,232 868 1,313 143 190
October 1,568 8 3,050 1,319 1,436 187 318
November 1,314 4 3,000 1,079 1,506 117 250
December 1,167 9 2,394 943 1,199 125 191 Accession Trends Bacteriology CYCADS
Molecular/ HATS Pathology Serology
Toxicology/ Nutrition Virology
2012 15,102 64 30,539 12,038 17,142 1,816 2,901
2011 14,618 * 24,942 12,725 16,736 1,452 2,203
2010 13,929 * 22,105 10,922 14,711 1,146 2,348
2009 12,940 * 19,606 11,155 15,581 1,007 2,753
2008 13,859 * 21,639 12,628 17,806 889 4,456
2007 16,366 * 30,872 15,582 20,327 1,117 9,982
2006 16,110 * 20,243 15,422 20,524 1,357 16,608 * CYCADS section was newly formed in 2012. See more on page 10.
13 ISU Veterinary Diagnostic Laboratory
Tests
2012 Tests Bacteriology CYCADS Molecular/
HATS Pathology Serology Toxicology/
Nutrition Virology
Total 159,009 2,025 194,275 92,503 580,338 7,418 3,839
January 11,697 13,891 7,488 42,639 482 295
February 11,133 14,856 6,306 35,662 274 241
March 13,178 17,703 8,818 50,767 827 355
April 12,590 1,780 14,244 7,495 44,878 777 257
May 15,072 16,010 9,900 54,576 845 571
June 13,374 5 16,155 8,415 48,792 531 296
July 18,053 3 14,910 5,485 39,340 546 239
August 12,818 1 18,757 6,599 43,428 794 357
September 8,129 8 12,526 3,981 34,832 373 225
October 14,616 188 16,820 9,379 61,064 875 293
November 13,534 39 22,549 11,632 68,990 884 411
December 14,815 1 15,854 7,008 55,370 210 299 Submission Trends
Number of Tests Number of Accessions
2012 1,039,407 52,487
2011 928,822 47,609
2010 716,864 41,770
2009 684,957 39,480
2008 1,157,388 41,957
2007 1,039,032 47,957
2006 1,330,084 47,238
2005 1,005,301 38,907
14 ISU Veterinary Diagnostic Laboratory
Species Submission Data
2012 Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Total
Total 4,171 4,268 4,771 4,514 4,642 4,292 3,878 4,502 3,856 4,948 4,739 3,906 52,487
Count of species 37
Porcine 2,752 2,767 2,916 2,746 2,833 2,665 2,482 2,831 2,493 3,325 3,206 2,555 33,571
Bovine 585 646 848 723 721 627 429 534 509 665 655 539 7,481
Canine 325 294 319 321 293 267 243 320 244 315 300 266 3,507
Avian 243 290 247 263 277 304 339 312 236 271 274 303 3,359
Equine 88 100 246 278 302 228 177 174 177 151 100 64 2,085
Feline 68 44 65 70 87 63 69 94 50 75 74 70 829
Non-Animal 27 23 27 17 23 20 20 62 45 44 39 32 379
Bat 13 10 10 9 29 19 25 77 15 10 13 18 248
Caprine 23 22 26 27 13 24 18 28 21 10 16 18 246
Ovine 11 30 18 21 16 19 19 13 4 10 4 11 176
Unknown 3 6 15 8 6 13 7 12 2 11 17 7 107
Rodent 13 5 5 5 10 8 16 15 4 6 7 2 96
Cervidae 2 3 5 9 3 11 3 5 25 14 6 6 92
Procyonidae 5 8 8 4 9 5 2 2 9 6 9 4 71
Camelid 5 7 3 5 4 2 3 6 3 11 2 2 53
Lagomorpha 4 3 1 1 4 2 3 2 8 1 2 31
Mephitidae 1 2 2 1 3 5 8 2 2 4 30
Mustelid 1 1 2 7 3 1 1 2 1 19
Marsupial 1 2 2 3 2 1 5 1 17
Primate 1 1 3 2 1 1 4 13
Fish 2 2 2 3 1 10
Snake 4 3 2 1 10
Bison 2 1 1 1 3 1 9
Amphibian 1 1 1 1 1 1 2 8
Antelope 3 2 3 8
Bovine Exotic 1 1 1 2 1 1 7
Bear 1 2 1 4
Porcine Exotic 1 1 1 1 4
Reptilian 1 1 1 1 4
Insectivora 1 2 3
Aquatic Mammal 1 1 2
Exotic 1 1 2
Feline Exotic 2 2
Canine Exotic 1 1
Crocodilian 1 1
Equine Exotic 1 1
Ovine Exotic 1 1
15 ISU Veterinary Diagnostic Laboratory
Top 10 species 2006 2007 2008 2009 2010 2011 2012 Total Submissions 47,238 47,957 41,957 39,480 41,770 47,609 52,487
Porcine 28,109 27,847 23,575 22,991 24,589 28,933 33,571
Bovine 7,384 8,275 7,479 6,211 6,393 7,329 7,481
Canine 3,677 3,794 3,694 3,665 3,468 3,588 3,507
Avian 2,563 2,750 2,324 1,857 2,813 3,322 3,359
Equine 2,833 2,646 2,235 2,146 2,074 2,125 2,085
Feline 960 984 913 897 783 758 829
Non-Animal 181 198 268 387 364 267 379
Caprine 223 238 241 203 208 241 248
Bat 269 265 293 295 230 230 246
Ovine 223 239 197 161 161 186 176
Porcine
Bovine
Canine
Avian
Equine Feline
Non-Animal
Caprine Bat
Ovine
Top 10 Species Submitted in 2012
16 ISU Veterinary Diagnostic Laboratory
Surveillance Testing
2012 Number of tests
Total 40,373
Avian influenza AGID - 1 23,773
Avian influenza AGID - EY 2,100
Avian influenza ELISA 808
Avian Pneumovirus ELISA 132
BSE Surveillance 16
CWD (NVSL) 16
PCR - Avian influenza virus 98
PCR - CSFV surveillance 1,312
PCR - Influenza A - NAHLN surveillance 1,418
PCR - Influenza A surv subtype 212
PRVgB - ELISA SCREEN NAHLN surveillance 9,716
Rivanol Confirmatory Testing 24
Virus isolation - Avian influenza virus 399
Virus isolation - Swine influenza NAHLN surveillance 349
17 ISU Veterinary Diagnostic Laboratory
Bacteriology Testing
Bacteriology trends 2007 2008 2009 2010 2011 2012
Antinuclear Antibody 5 2
Bacterial ID 15,968 20,805 17,527 14,196 18,657 22,657
Bacterial ID from mailed culture 272 1,109 1,215 822 430 895
Brachyspira Culture 1,442 3,139 7,215 7,358 8,962 16,157
Campy and Trich Culture 935 1,582 715 1,025 1,309 1,241
Campylobacter Culture 420 506 535 505 906 581
Chlamydia - AgELISA 52 110 70 34 49 8 Clin Micro Culture - Aerobic and
Anaerobic 3,084 3,559 3,192 2,908 1,681 1,709
Clostridium difficile culture 76 74 71 130 246 32
Clostridium difficile toxin A/B - ELISA 459 495 405 526 526 525
Clostridium FA 30 36 45 48 50 66
Coliform Test (Membrane Filter) 328 498 460 48 58 47 Culture of feed for Clostridium
perfringens 3 2 1 2
Culture Saved 958 1,184 1,239 957 940 1,059
Direct Smear 82 68 59 64 65 53
E. coli K99 LA Typing 502 394 100 22 23 3
Enrichments 8,228 8,192 7,489 6,727 8,241 8,582
Fungal Culture 263 341 287 320 301 367
Johne's Liquid Culture 915 965 338 172 719 305
Johne's Liquid Culture pooled 145 82 99 83
Leptospira FA 108 26 8 2 7 Methicillin Resistant Staph Protein
(PBP2A) 28 95
Milk Culture 2,863 11,608 5,274 5,882 7,431 12,783
Milk Culture – Bulk Tank 74 122 189 44 28 4
Mycoplasma Culture 318 879 969 544 337 163
Mycoplasma Id 25 2 1 2
Mycoplasma Milk Culture 3 26 2 4
Parvo Virus - ELISA 4 4
Poultry - Salmonella Environmental 3,353 3,306 4,636 11,896 28,992 23,195
Rheumatoid factor agglutination 1 1
Routine Culture 49,741 41,378 35,836 33,226 46,238 49,911
Salmonella Id 1,539 1,364 979 830 1,291 1,120
Salmonella Identification (NVSL FDA) 124 Serogroup/serotype (APP, Salm,
Strep) 271 239 204 161 107 172
Slant Request 238 330 229 329 240 219 Special Culture (Nasal, Feces, Semen,
Other) 179 757 1,021 1,106 425 3,360
SRID for IgG 25 124 9 8 13
Standard Plate Count 450 152 271 192 455 1,812
Trichomonas Culture 294 171 226 28 180 394
18 ISU Veterinary Diagnostic Laboratory
Bacteriology trends 2007 2008 2009 2010 2011 2012
Susceptibilities
Avian MIC 115 102 76 106 94 101
Bovine Mastitis Kirby Bauer 27 30 6
Bovine Respiratory Kirby Bauer 24 19 6 1
Companion/Equine MIC 199 262 437 895 1,605 1,932
Equine Kirby Bauer 209 233 138 128 23 2
E-TEST 66 56 49 61 80 54
Food Animal MIC 8,324 7,879 6,100 5,527 7,782 8,190
Individual Antibiotic 239 680 837 988
Mastitis MIC 108 76 96 105 88 118
Multiresistant Kirby Bauer 125 61 45 41 23 12
Small Animal Kirby Bauer 890 775 691 446 52 7
2012 Salmonella serotypes isolated Avian Bovine
Porcine
Group B
Salmonella typhimurium var 5- 3 7 210
Salmonella derby 2 122
Salmonella agona 1 5 86
Salmonella typhimurium 10 5 53
Salmonella 4,5,12:i: 5 87
Salmonella heidelberg 4 35
Salmonella schwarzengrund 12
Salmonella reading 8
Salmonella brandenburg 6
Salmonella bredeney 3
Salmonella saint-paul 1
Group C1
Salmonella infantis 2 63
Salmonella ohio 25
Salmonella mbandaka 2 14
Salmonella rissen 2 10
Salmonella altona 6
Salmonella tennessee 4
Salmonella litchfield 4
Salmonella choleraesuis_(kunzendorf) 3
Salmonella livingstone 3
Salmonella braenderup 2
Salmonella montevideo 11 2
Salmonella thompson 2 1 2
Salmonella choleraesuis 1
Salmonella hadar 1
Salmonella kentucky 5
Group C2
Salmonella muenchen 1 16
Salmonella manhattan 5
Salmonella bovismorbificans 5
Salmonella newport 7 4
19 ISU Veterinary Diagnostic Laboratory
2012 Salmonella serotypes isolated Avian Bovine
Porcine
Salmonella muechen
Group D
Salmonella enteritidis 11 5
Salmonella javiana 1
Salmonella berta 1
Salmonella duisburg 1
Salmonella dublin 28
Group E
Salmonella anatum 1 7 40
Salmonella senftenberg 2 17
Salmonella london 13
Salmonella uganda 3 6
Salmonella krefeld 6
Salmonella give 1 3
Salmonella meleagridis 2 3
Salmonella muenster 1 1 1
Salmonella uganda_var_15+ 1
Salmonella liverpool 1
Group K
Salmonella cerro 5 2
Group Poly/Other Salmonella johannesburg 1 39
Salmonella worthington 22
Untypeable
Salmonella rough O:-:1,5 6
Salmonella rough_O:c:1,5 5
Salmonella 6,7:-:1,5 1 3
Salmonella 6,7:nonmotile 2
Salmonella barranquilla 1 2
Salmonella panama 2
Salmonella 6,7:-:I,w 2
Salmonella 6,7 nonmotile 1
Salmonella bere 1
Salmonella molade 1
Salmonella oranienburg 1
Salmonella rough O:i:z13:1,5 1
Salmonella rough_O:d:lw 1
Salmonella rough_O:eh:1,2 1
Salmonella rough_O:fg:- 1
Salmonella rough_O:i:1,2 1
Salmonella rough_O:z:lw 1
Salmonella soerenga 1
Salmonella 6,7:c:- 1
Salmonella 8,20:nonmotile 1
Salmonella III_38:k:z35 1
Salmonella rough_O:e,h:1,6 1
Salmonella rough_O:gms:- 1
20 ISU Veterinary Diagnostic Laboratory
Bacteriology APP serotypes isolated from porcine 2008 2009 2010 2011 2012
APP untypeable 49 43 42 34 68
APP Serotype 7 37 43 35 18 19
APP Serotype 1 7 10 4 4 6
APP Serotype 3 6 8 3 4 6 APP Serotype 12 2 6 1 4
APP Serotype 2 1 2 1 1
APP Serotype 8 3 3 2 1
APP Serotype 11 1
APP Serotype 4 1
APP Serotype 5 29 7 2
21 ISU Veterinary Diagnostic Laboratory
Antimicrobial Susceptibility Profiles
• Note: The susceptibility information presented below is a summary of data gathered at ISU VDL for the time period listed. The information may be useful to understand susceptibility trends or as an aid in making clinical decisions, but may not be accurate for specific disease situations.
• In vitro antimicrobial test results do not represent therapeutic recommendations from the VDL or personnel therein. Extra/Off label usage of an
antimicrobial which is limited/prohibited for certain species may result in legal action by FDA-CVM
• Data is reported as: % susceptible (# isolates tested) – not all bacteria isolated at ISU VDL have been tested for antimicrobial susceptibility
Avian 2010-2012 Susceptibility profile of Avian pathogens received at ISU VDL
Data reported as: % susceptible (# isolates tested)1
Antibiotic E coli G ana P mult Salm B Salm D Salm sp
Amoxicillin 79% (143) 100% (10) 100% (3) 96% (23) 100% (22) 91% (32)
Ceftiofur 94% (143) 100% (10) 100% (3) 100% (23) 100% (22) 94% (32)
Clindamycin 0% (143) 0% (10) 0% (3) 0% (23) 0% (22) 0% (32)
Enrofloxacin 97% (143) 100% (10) 100% (3) 100% (23) 100% (22) 100% (32)
Erythromycin 0% (143) 10% (10) 0% (3) 0% (23) 0% (22) 0% (32)
Florfenicol 46% (143) 100% (10) 100% (3) 48% (23) 18% (22) 63% (32)
Gentamicin 81% (143) 100% (10) 100% (3) 96% (23) 100% (22) 78% (32)
Neomycin 87% (143) 70% (10) 100% (3) 96% (23) 100% (22) 91% (32)
Novobiocin 0% (143) 10% (10) 67% (3) 0% (23) 0% (22) 0% (32)
Oxytetracycline 44% (143) 20% (10) 67% (3) 70% (23) 100% (22) 47% (32)
Penicillin 1% (143) 0% (10) 100% (3) 0% (23) 0% (22) 0% (32)
Spectinomycin 2% (143) 0% (10) 0% (3) 0% (23) 0% (22) 0% (32)
Streptomycin 60% (143) 90% (10) 0% (3) 30% (23) 100% (22) 38% (32)
Sulfadimethoxine 53% (143) 70% (10) 33% (3) 17% (23) 32% (22) 41% (32)
Sulphathiazole 62% (143) 40% (10) 33% (3) 70% (23) 95% (22) 69% (32)
Tetracycline 44% (143) 20% (10) 67% (3) 78% (23) 95% (22) 47% (32)
Trimethoprim/Sulphamethoxazole 94% (143) 90% (10) 100% (3) 100% (23) 100% (22) 100% (32)
Tylosin (Tartrate/Base) 0% (143) 10% (10) 0% (3) 0% (23) 0% (22) 0% (32)
2 See Salmonella serotype table on page 18 for most common serotypes isolated within each group
22 ISU Veterinary Diagnostic Laboratory
Bovine 2010-2012 Susceptibility profile of Bovine pathogens received at ISU VDL
Data reported as: % susceptible (# isolates tested)1
Antibiotic B tre E coli H som M bov M haem P mult Salm B Salm C2 Salm D Salm sp
Ampicillin 56% (32) 26% (893) 89% (229) 100% (54) 58% (613) 98% (470) 46% (89) 56% (32) 18% (131) 88% (156)
Ceftiofur 88% (32) 54% (893) 100% (229) 100% (54) 99% (613) 100% (470) 74% (89) 56% (32) 20% (131) 90% (156)
Chlortetracycline 50% (32) 11% (893) 98% (229) 100% (54) 62% (613) 94% (470) 36% (89) 38% (32) 3% (131) 72% (156)
Clindamycin 6% (32) 0% (893) 47% (229) 7% (54) 0% (613) 0% (470) 0% (89) 0% (32) 0% (131) 0% (156)
Danofloxacin 34% (32) 50% (893) 74% (229) 2% (54) 54% (613) 82% (470) 75% (89) 81% (32) 82% (131) 79% (156)
Enrofloxacin 47% (32) 60% (893) 78% (229) 100% (54) 57% (613) 90% (470) 96% (89) 100% (32) 100% (131) 99% (156)
Florfenicol 75% (32) 9% (893) 94% (229) 98% (54) 75% (613) 92% (470) 29% (89) 38% (32) 4% (131) 47% (156)
Gentamicin 88% (32) 68% (893) 21% (229) 100% (54) 63% (613) 76% (470) 90% (89) 100% (32) 97% (131) 96% (156)
Neomycin 44% (32) 33% (893) 2% (229) 100% (54) 50% (613) 22% (470) 74% (89) 94% (32) 44% (131) 96% (156)
Oxytetracycline 16% (32) 9% (893) 34% (229) 100% (54) 37% (613) 58% (470) 35% (89) 38% (32) 3% (131) 72% (156)
Penicillin 13% (32) 0% (893) 81% (229) 2% (54) 21% (613) 71% (470) 0% (89) 0% (32) 0% (131) 0% (156)
Spectinomycin 3% (32) 0% (893) 50% (229) 93% (54) 55% (613) 66% (470) 0% (89) 0% (32) 0% (131) 0% (156)
Sulfadimethoxine 38% (32) 11% (893) 12% (229) 98% (54) 13% (613) 7% (470) 2% (89) 6% (32) 2% (131) 29% (156)
Tiamulin 56% (32) 1% (893) 100% (229) 100% (54) 94% (613) 72% (470) 0% (89) 0% (32) 0% (131) 0% (156)
Tilmicosin 47% (32) 0% (893) 90% (229) 98% (54) 44% (613) 66% (470) 0% (89) 0% (32) 0% (131) 0% (156)
Trimethoprim/Sulphamethoxazole 75% (32) 43% (893) 94% (229) 100% (54) 0% (613) 1% (470) 82% (89) 97% (32) 74% (131) 97% (156)
Tulathromycin NI NI 79% (229) NI 58% (613) 83% (470) NI 0% (32) NI NI
Tylosin (Tartrate/Base) 9% (32) NI 59% (229) NI 1% (613) 1% (470) NI 0% (32) NI NI
2 See Salmonella serotype table on page 18 for most common serotypes isolated within each group
23 ISU Veterinary Diagnostic Laboratory
Bovine Mastitis 2010-2012 Susceptibility profile of Mastitis pathogens received at ISU VDL
Data reported as: % susceptible (# isolates tested)1
Antibiotic E coli E faem Ente K pneu P mult S aur S dysg S epi S ube
Ampicillin 80% (49) 100% (2) 63% (8) 0% (10) 100% (2) 78% (64) 100% (27) 83% (36) 78% (40)
Ceftiofur 96% (49) 50% (2) 88% (8) 100% (10) 100% (2) 100% (64) 100% (27) 94% (36) 85% (40)
Cephalothin 82% (49) 50% (2) 0% (8) 100% (10) 100% (2) 100% (64) 100% (27) 94% (36) 100% (40)
Erythromycin 0% (49) 0% (2) 0% (8) 0% (10) 0% (2) 97% (64) 93% (27) 83% (36) 75% (40)
Oxacillin 0% (49) 0% (2) 0% (8) 0% (10) 50% (2) 94% (64) 96% (27) 6% (36) 78% (40)
Penicillin 0% (49) 50% (2) 0% (8) 0% (10) 100% (2) 77% (64) 100% (27) 81% (36) 35% (40)
Penicillin/Novobiocin 0% (49) NI NI 0% (10) NI 97% (64) 100% (27) 94% (36) 98% (40)
Pirlimycin 0% (49) 50% (2) 0% (8) 0% (10) 0% (2) 97% (64) 93% (27) 86% (36) 78% (40)
Sulfadimethoxine 43% (49) 0% (2) 0% (8) 30% (10) 0% (2) 88% (64) 59% (27) 69% (36) 0% (40)
Tetracycline 47% (49) 50% (2) 50% (8) 80% (10) 50% (2) 89% (64) 19% (27) 61% (36) 65% (40)
3 Isolates resistant to oxacillin are interpreted as potentially methicillin resistant.
24 ISU Veterinary Diagnostic Laboratory
Canine 2010-2012 Susceptibility profile of Canine pathogens received at ISU VDL
Data reported as: % susceptible (# isolates tested)1
Antibiotic B bron E coli E fael E faem Ente K pneu P aer P mult Pseu S aur S can S pint
Amikacin 100% (25) 96% (621) 22% (182) 18% (39) 100% (107) 96% (52) 91% (196) 100% (33) 88% (99) 96% (28) 2% (165) 100% (665)
Amoxicillin/Clavulanic Acid 80% (25) 74% (621) 98% (182) 41% (39) 38% (107) 67% (52) 1% (196) 100% (33) 45% (99) 75% (28) 99% (165) 85% (665)
Ampicillin 28% (25) 58% (621) 96% (182) 38% (39) 39% (107) 13% (52) 0% (196) 100% (33) 41% (99) 32% (28) 98% (165) 47% (665)
Cefazolin 16% (25) 81% (621) 4% (182) 10% (39) 25% (107) 67% (52) 1% (196) 100% (33) 33% (99) 75% (28) 100% (165) 85% (665)
Cefovecin 16% (25) 82% (621) 3% (182) 3% (39) 79% (107) 75% (52) 0% (196) 100% (33) 38% (99) 75% (28) 98% (165) 82% (665)
Cefoxitin 16% (25) 84% (621) 1% (182) 0% (39) 43% (107) 69% (52) 1% (196) 100% (33) 36% (99) 54% (28) 99% (165) 83% (665)
Cefpodoxime 16% (25) 81% (621) 15% (182) 8% (39) 82% (107) 81% (52) 0% (196) 97% (33) 37% (99) 75% (28) 98% (165) 82% (665)
Ceftiofur 12% (25) 81% (621) 4% (182) 10% (39) 82% (107) 81% (52) 0% (196) 100% (33) 38% (99) 75% (28) 99% (165) 82% (665)
Cephalothin 83% (6) 63% (51) 7% (95) 21% (14) 10% (10) 0% (2) 0% (22) 100% (2) 27% (11) 69% (16) 98% (106) 87% (378)
Chloramphenicol 96% (25) 81% (621) 92% (182) 95% (39) 76% (107) 81% (52) 2% (196) 100% (33) 42% (99) 71% (28) 96% (165) 86% (665)
Clindamycin 8% (25) 0% (621) 1% (182) 36% (39) 0% (107) 0% (52) 1% (196) 3% (33) 6% (99) 89% (28) 86% (165) 73% (665)
Doxycycline 100% (25) 77% (621) 72% (182) 46% (39) 64% (107) 73% (52) 6% (196) 97% (33) 65% (99) 86% (28) 62% (165) 59% (665)
Enrofloxacin 92% (25) 86% (621) 32% (182) 0% (39) 93% (107) 87% (52) 45% (196) 100% (33) 59% (99) 82% (28) 46% (165) 77% (665)
Erythromycin 4% (25) 0% (621) 26% (182) 8% (39) 1% (107) 0% (52) 1% (196) 27% (33) 19% (99) 68% (28) 0% (165) 72% (665)
Gentamicin 84% (25) 86% (621) 52% (182) 10% (39) 96% (107) 88% (52) 72% (196) 100% (33) 83% (99) 96% (28) 35% (165) 81% (665)
Imipenem 100% (25) 100% (621) 98% (182) 36% (39) 99% (107) 100% (52) 95% (196) 100% (33) 96% (99) 71% (28) 100% (165) 85% (665)
Marbofloxacin 92% (25) 88% (621) 37% (182) 0% (39) 94% (107) 94% (52) 72% (196) 100% (33) 81% (99) 82% (28) 81% (165) 82% (665)
Oxacillin3 NI NI NI NI NI NI NI NI NI 75% (28) NI 80% (665)
Penicillin 4% (25) 0% (621) 96% (182) 38% (39) 0% (107) 0% (52) 0% (196) 85% (33) 0% (99) 21% (28) 98% (165) 31% (665)
Ticarcillin 60% (25) 64% (621) 8% (182) 18% (39) 76% (107) 17% (52) 89% (196) 100% (33) 72% (99) 75% (28) 99% (165) 83% (665)
Ticarcillin/Clavulanic Acid 96% (25) 77% (621) 7% (182) 15% (39) 87% (107) 81% (52) 89% (196) 100% (33) 78% (99) 75% (28) 99% (165) 84% (665) Trimethoprim/
Sulphamethoxazole 40% (25) 82% (621) 93% (182) 77% (39) 96% (107) 83% (52) 8% (196) 97% (33) 51% (99) 100% (28) 96% (165) 75% (665)
3 Isolates resistant to oxacillin are interpreted as potentially methicillin resistant.
25 ISU Veterinary Diagnostic Laboratory
Caprine / Ovine 2010-2012 Susceptibility profile of Caprine / Ovine pathogens received at ISU VDL
Data reported as: % susceptible (# isolates tested)1
Antibiotic B tre E coli M haem P mult Salm sp
Ampicillin 100% (5) 45% (33) 98% (44) 88% (8) 89% (18)
Ceftiofur 100% (5) 82% (33) 100% (44) 100% (8) 94% (18)
Chlortetracycline 40% (5) 12% (33) 95% (44) 88% (8) 83% (18)
Clindamycin 0% (5) 0% (33) 0% (44) 0% (8) 0% (18)
Danofloxacin 80% (5) 79% (33) 93% (44) 88% (8) 72% (18)
Enrofloxacin 100% (5) 82% (33) 98% (44) 88% (8) 94% (18)
Florfenicol 100% (5) 3% (33) 100% (44) 100% (8) 78% (18)
Gentamicin 100% (5) 79% (33) 100% (44) 100% (8) 94% (18)
Neomycin 100% (5) 45% (33) 89% (44) 88% (8) 100% (18)
Oxytetracycline 40% (5) 12% (33) 82% (44) 75% (8) 83% (18)
Penicillin 0% (5) 0% (33) 39% (44) 75% (8) 0% (18)
Spectinomycin 0% (5) 3% (33) 93% (44) 100% (8) 0% (18)
Sulfadimethoxine 100% (5) 18% (33) 57% (44) 13% (8) 6% (18)
Tiamulin 100% (5) 0% (33) 95% (44) 100% (8) 0% (18)
Tilmicosin 100% (5) 0% (33) 98% (44) 100% (8) 0% (18)
Trimethoprim/Sulphamethoxazole 100% (5) 64% (33) 5% (44) 0% (8) 100% (18)
Tulathromycin NI NI 93% (44) 100% (8) 0% (18)
26 ISU Veterinary Diagnostic Laboratory
Equine 2010-2012 Susceptibility profile of Equine pathogens received at ISU VDL
Data reported as: % susceptible (# isolates tested)1
Antibiotic A equ B bron P aer Past R equ S aur S equi S equs S zoo Salm B
Amikacin 95% (19) 100% (4) 100% (6) 100% (3) 88% (8) 100% (15) 0% (7) 11% (19) 1% (105) 100% (9)
Ampicillin 100% (19) 25% (4) 0% (6) 100% (3) 13% (8) 67% (15) 100% (7) 100% (19) 96% (106) 89% (9)
Azithromycin NI NI NI 100% (3) NI 93% (14) 100% (6) 100% (19) 97% (100) NI
Cefazolin 89% (19) 0% (4) 0% (6) 100% (3) 13% (8) 100% (15) 100% (7) 100% (19) 99% (105) 89% (9)
Ceftazidime 100% (19) 100% (4) 100% (6) 100% (3) 13% (8) 100% (14) 100% (6) 100% (19) 100% (100) 89% (9)
Ceftiofur 95% (19) 0% (4) 0% (6) 100% (3) 13% (8) 100% (15) 100% (7) 100% (19) 96% (106) 89% (9)
Chloramphenicol 89% (19) 100% (4) 0% (6) 100% (3) 88% (8) 100% (15) 100% (7) 100% (19) 98% (105) 89% (9)
Clarithromycin NI NI NI NI 100% (8) 93% (14) NI NI 0% (100) NI
Doxycycline 100% (19) 100% (4) 0% (6) 100% (3) 88% (8) 87% (15) 100% (7) 89% (19) 79% (105) 89% (9)
Enrofloxacin 95% (19) 100% (4) 83% (6) 100% (3) 38% (8) 93% (15) 0% (7) 74% (19) 21% (106) 100% (9)
Erythromycin 11% (19) 0% (4) 0% (6) 67% (3) 88% (8) 87% (15) 86% (7) 95% (19) 93% (105) 0% (9)
Gentamicin 95% (19) 100% (4) 50% (6) 100% (3) 100% (8) 80% (15) 14% (7) 47% (19) 2% (106) 100% (9)
Imipenem 100% (19) 100% (4) 83% (6) 100% (3) 100% (8) 93% (15) 100% (7) 100% (19) 100% (105) 100% (9)
Oxacillin3 NI NI 0% (6) NI NI 100% (15) NI NI NI NI
Penicillin 0% (19) 0% (4) 0% (6) 0% (3) 0% (8) 73% (15) 100% (7) 95% (19) 96% (106) 0% (9)
Tetracycline 100% (19) 100% (4) 0% (6) 100% (3) 88% (8) 71% (14) 100% (6) 63% (19) 21% (100) 89% (9)
Ticarcillin 95% (19) 75% (4) 67% (6) 100% (3) 13% (8) 93% (15) 100% (7) 100% (19) 100% (105) 89% (9)
Ticarcillin/Clavulanic Acid 95% (19) 100% (4) 67% (6) 100% (3) 13% (8) 100% (15) 100% (7) 100% (19) 100% (105) 89% (9)
Trimethoprim/Sulphamethoxazole 100% (19) 50% (4) 0% (6) 100% (3) 63% (8) 80% (15) 100% (7) 100% (19) 93% (106) 89% (9)
3 Isolates resistant to oxacillin are interpreted as potentially methicillin resistant.
27 ISU Veterinary Diagnostic Laboratory
E-test 2010-2012 Susceptibility profile of E-Test pathogens received at ISU VDL
Data reported as: % susceptible (# isolates tested)1
Antibiotic Amy Bact Clos Fus Pec Pes
Cefoxitin 100% (20) 92% (85) 92% (13) 94% (33) 89% (9) 100% (11)
Clindamycin 95% (20) 85% (85) 69% (13) 94% (33) 89% (9) 73% (11)
Metronidazole 70% (20) 95% (85) 92% (13) 100% (33) 100% (9) 82% (11)
Penicillin 90% (20) 67% (85) 92% (13) 91% (33) 100% (9) 91% (11)
28 ISU Veterinary Diagnostic Laboratory
Feline 2010-2012 Susceptibility profile of Feline pathogens received at ISU VDL
Data reported as: % susceptible (# isolates tested)1
Antibiotic B bron E coli E fael E faem Ente K pneu P aer P mult Pseu S aur S can S pint
Amikacin 100% (16) 99% (81) 44% (45) 18% (11) 94% (16) 100% (2) 100% (33) 88% (34) 83% (18) 100% (18) 15% (13) 100% (20) Amoxicillin/Clavulanic
Acid 81% (16) 80% (81) 98% (45) 27% (11) 44% (16) 100% (2) 0% (33) 100% (34) 61% (18) 94% (18) 100% (13) 90% (20)
Ampicillin 25% (16) 64% (81) 96% (45) 27% (11) 50% (16) 0% (2) 3% (33) 100% (34) 56% (18) 39% (18) 100% (13) 65% (20)
Cefazolin 0% (16) 94% (81) 2% (45) 0% (11) 19% (16) 100% (2) 0% (33) 100% (34) 33% (18) 94% (18) 100% (13) 90% (20)
Cefovecin 0% (16) 94% (81) 2% (45) 0% (11) 75% (16) 100% (2) 0% (33) 97% (34) 33% (18) 94% (18) 100% (13) 90% (20)
Cefoxitin 0% (16) 93% (81) 0% (45) 0% (11) 38% (16) 100% (2) 0% (33) 97% (34) 50% (18) 61% (18) 100% (13) 85% (20)
Cefpodoxime 0% (16) 93% (81) 16% (45) 0% (11) 75% (16) 100% (2) 0% (33) 97% (34) 39% (18) 89% (18) 100% (13) 90% (20)
Ceftiofur 0% (16) 95% (81) 4% (45) 0% (11) 75% (16) 100% (2) 6% (33) 100% (34) 39% (18) 89% (18) 100% (13) 90% (20)
Cephalothin 0% (3) 82% (11) 4% (27) 25% (4) 0% (1) 0% (5) 100% (9) 33% (3) 90% (10) 100% (6) 86% (14)
Chloramphenicol 88% (16) 93% (81) 93% (45) 100% (11) 75% (16) 100% (2) 3% (33) 100% (34) 56% (18) 78% (18) 100% (13) 100% (20)
Clindamycin 0% (16) 0% (81) 4% (45) 9% (11) 0% (16) 0% (2) 0% (33) 0% (34) 6% (18) 89% (18) 85% (13) 80% (20)
Doxycycline 100% (16) 90% (81) 82% (45) 45% (11) 69% (16) 100% (2) 15% (33) 97% (34) 83% (18) 94% (18) 69% (13) 70% (20)
Enrofloxacin 81% (16) 98% (81) 38% (45) 9% (11) 88% (16) 100% (2) 76% (33) 100% (34) 83% (18) 94% (18) 38% (13) 75% (20)
Erythromycin 0% (16) 0% (81) 20% (45) 9% (11) 0% (16) 0% (2) 0% (33) 12% (34) 28% (18) 50% (18) 0% (13) 60% (20)
Gentamicin 100% (16) 100% (81) 71% (45) 9% (11) 94% (16) 100% (2) 91% (33) 97% (34) 89% (18) 100% (18) 62% (13) 90% (20)
Imipenem 100% (16) 100% (81) 100% (45) 27% (11) 94% (16) 100% (2) 97% (33) 100% (34) 89% (18) 94% (18) 100% (13) 90% (20)
Marbofloxacin 100% (16) 98% (81) 40% (45) 9% (11) 94% (16) 100% (2) 97% (33) 100% (34) 89% (18) 94% (18) 77% (13) 75% (20)
Oxacillin3 NI NI NI NI NI NI NI NI NI 94% (18) NI 90% (20)
Penicillin 0% (16) 0% (81) 96% (45) 27% (11) 0% (16) 0% (2) 0% (33) 65% (34) 0% (18) 33% (18) 100% (13) 40% (20)
Ticarcillin 56% (16) 75% (81) 11% (45) 9% (11) 69% (16) 0% (2) 94% (33) 100% (34) 72% (18) 94% (18) 100% (13) 90% (20) Ticarcillin/Clavulanic
Acid 100% (16) 91% (81) 9% (45) 9% (11) 75% (16) 100% (2) 94% (33) 100% (34) 72% (18) 94% (18) 100% (13) 90% (20) Trimethoprim/
Sulphamethoxazole 75% (16) 95% (81) 93% (45) 82% (11) 94% (16) 100% (2) 18% (33) 94% (34) 72% (18) 100% (18) 100% (13) 75% (20)
3 Isolates resistant to oxacillin are interpreted as potentially methicillin resistant.
29 ISU Veterinary Diagnostic Laboratory
Porcine 2012 Susceptibility profile of Porcine pathogens received at ISU VDL
Data reported as: % susceptible (# isolates tested)1
Antibiotic A suis APP B bron E coli Erys H ecol HPS Pmul A Pmul D S suis Salm B2 Salm C12 Salm sp
Ampicillin 95% (257) 90% (107) 5% (652) 32% (154) 94% (18) 27% (1141) 98% (565) 99% (862) 98% (365) 97% (2026) 33% (613) 64% (157) 63% (249)
Ceftiofur 100% (257) 95% (107) 0% (652) 71% (154) 83% (18) 65% (1141) 100% (565) 99% (862) 100% (365) 99% (2026) 79% (613) 76% (157) 80% (249)
Chlortetracycline 98% (257) 89% (107) 99% (652) 16% (154) 11% (18) 8% (1141) 100% (565) 99% (862) 97% (365) 18% (2026) 16% (613) 52% (157) 46% (249)
Clindamycin 0% (257) 2% (107) 0% (652) 0% (154) 67% (18) 0% (1141) 7% (565) 0% (862) 0% (365) 20% (2026) 0% (613) 0% (157) 0% (249)
Enrofloxacin 99% (257) 99% (107) 97% (652) 78% (154) 83% (18) 90% (1141) 99% (565) 100% (862) 100% (365) 97% (2026) 95% (613) 96% (157) 91% (249)
Florfenicol 99% (257) 98% (107) 31% (652) 14% (154) 11% (18) 17% (1141) 99% (565) 100% (862) 100% (365) 98% (2026) 18% (613) 20% (157) 35% (249)
Gentamicin 98% (257) 3% (107) 99% (652) 76% (154) 6% (18) 66% (1141) 83% (565) 98% (862) 99% (365) 96% (2026) 85% (613) 76% (157) 77% (249)
Neomycin 93% (257) 2% (107) 94% (652) 73% (154) 6% (18) 58% (1141) 43% (565) 90% (862) 93% (365) 75% (2026) 79% (613) 84% (157) 81% (249)
Oxytetracycline 79% (257) 16% (107) 98% (652) 14% (154) 11% (18) 7% (1141) 94% (565) 23% (862) 55% (365) 4% (2026) 13% (613) 51% (157) 43% (249)
Penicillin 2% (257) 18% (107) 0% (652) 0% (154) 94% (18) 0% (1141) 25% (565) 87% (862) 97% (365) 81% (2026) 0% (613) 0% (157) 0% (249)
Spectinomycin 0% (257) 6% (107) 0% (652) 1% (154) 72% (18) 2% (1141) 55% (565) 1% (862) 0% (365) 19% (2026) 0% (613) 0% (157) 0% (249)
Sulfadimethoxine 92% (257) 38% (107) 24% (652) 36% (154) 11% (18) 24% (1141) 27% (565) 24% (862) 27% (365) 33% (2026) 3% (613) 32% (157) 8% (249)
Tiamulin 96% (257) 93% (107) 0% (652) 0% (154) 67% (18) 1% (1141) 97% (565) 79% (862) 29% (365) 87% (2026) 0% (613) 0% (157) 0% (249)
Tilmicosin 95% (257) 93% (107) 2% (652) 0% (154) 89% (18) 0% (1141) 90% (565) 92% (862) 43% (365) 24% (2026) 0% (613) 0% (157) 0% (249) Trimethoprim/ Sulphamethoxazole 98% (257) 0% (107) 26% (652) 71% (154) 17% (18) 70% (1141) 92% (565) 0% (862) 0% (365) 97% (2026) 86% (613) 83% (157) 92% (249)
Tulathromycin 0% (257) 99% (107) 98% (652) 0% (154) 0% (18) 0% (1141) 0% (565) 99% (862) 99% (365) 0% (2026) 0% (613) 0% (157) 0% (249)
Tylosin (Tartrate/Base) 0% (257) 2% (107) 0% (652) 0% (154) 0% (18) 0% (1141) 0% (565) 0% (862) 0% (365) 0% (2026) 0% (613) 0% (157) 0% (249)
Carabadox 4 E coli Salm
>2 ug/ml <= 2 ug/ml >2 ug/ml <= 2 ug/ml
35% (596) 65%(596) 15%(324) 85%(324)
2 See Salmonella serotype table on page 18 for most common serotypes isolated within each group 4 A result of <=2 ug/ml for Carbadox is a conservative indicator of bacterial inhibition by this antimicrobial agent. The result shown is based on pharmacokinetic research
indicating an average Carbadox level of 4.5 mcg/ml in the small intestine of pigs fed a dose rate of 50 g/ton. (De Graff 1988).
30 ISU Veterinary Diagnostic Laboratory
Key:
1 Data is reported as: % susceptible (# isolates tested) – not all bacteria isolated at ISU VDL have been tested for antimicrobial susceptibility 2 See Salmonella serotype table on page 18 for most common serotypes isolated within each group
3 Isolates resistant to oxacillin are interpreted as potentially methicillin resistant. 4 A result of <=2 ug/ml for Carbadox is a conservative indicator of bacterial inhibition by this antimicrobial agent. The result shown is based on pharmacokinetic
research indicating an average Carbadox level of 4.5 mcg/ml in the small intestine of pigs fed a dose rate of 50 g/ton. (De Graff 1988). 5 Multidrug resistant isolates were found resistant to most classes of antimicrobial in the 1st round of testing. This table represents additional Disk Diffusion
testing for those isolates. NA Not applicable ND Not done NI No interpretation
A equ - Actinobacillus equuli H ecol - hemolytic E.coli S aur - Staphylococcus aureus
A suis - Actinobacillus suis H som - Histoplilus somni S beta- Beta Streptococcus species
Abua - Acinetobacter species HPS - Haemophilus parasuis S can - Streptococcus canis
Amy - Actinomyces species K pneu - Klebsiella pneumoniae S chol - Salmonella choleraesuis
APP - Actinobacillus pleuropneumoniae M bov - Moraxella bovis S dysg - Streptococcus dysgalactiae
B bron - Bordetella bronchiseptica M haem - Mannheimia haemolytica S epi- Staphylococcus epidermidis
B tre - Bibersteinia trehalosi (formerly Pasteurella trehalosi) P aer - Pseudomonas aeruginosa S equi - Streptococcus equi
Bact - Bacteriodes group P cab - Pasteurella caballi S equs - Streptococcus equisimillis
C diff - Clostridium difficile P mult - Pasteurella multocida S pint - Staph pseudintermedius
C perf - Clostridium perfringens Past - Pasteurella species S suis - Streptococcus suis
Clos - Clostridium species Pec - Peptocococcus species S ube - Streptococcus uberis
E coli - Escherichia coli Pes - Peptostreptococcus species S zoo - Streptococcus zooepidemicus
E fael - Enterococcus faecalis Pmul A - Pasteurella multocida Type A Salm sp- Salmonella species
E faem - Enterococcus faecium Pmul D - Pasteurella multocida Type D Salm B - Salmonella species group B
Enc - Enterococcus species Prot - Proteus species Salm C1 - Salmonella species group C1
Ente - Enterobacter species Prp - Propionibacterium species Salm C2 - Salmonella species group C2
Erys – Erysipelothrix Pseu - Pseudomonas species Salm D - Salmonella species group D
Fus - Fusobacterium R equ - Rhodococcus equi Salm E - Salmonella species group E
G ana - Gallibacterium anatis
31 ISU Veterinary Diagnostic Laboratory
CYCADS Testing
CYCADS trends* 2007 2008 2009 2010 2011 2012 AB Panel without CA or AMG using LCMS 68
Antibiotic Panel using LCMS 1
Individual Antibiotic using LCMS 171
Meloxicam by LCMS: Research 77
Substance P by I.A.: Research 1,708 * CYCADS section was newly formed in 2012
Molecular and Health Assurance (HATS) Testing
Molecular Trends 2007 2008 2009 2010 2011 2012
PCR Totals 83,761 83,259 83,651 97,521 140,661 173,000 PCR - Actinobacillus pleuropneumoniae 1 3 7 1 8
PCR - Actinobacillus suis 16
PCR - Avian influenza virus 1,270 1,545 1,173 2,163 1,027 68 PCR - Avian paramyxovirus-1 matrix
gene 4 36
PCR - Bovine Coronavirus 901 1,228 1,053 2,273 97 55 PCR - Bovine Respiratory Bacterial
Panel 35 PCR - Bovine Respiratory Viral and
Bacterial Panel 80
PCR - Bovine Respiratory Viral Panel 48 PCR - Brachyspira hyodysenteriae or
B.pilosicoli 4 156 328 453 507 33
PCR - Brachyspira species panel 207 251
PCR - BRSV 160 245 164 240 258 187
PCR - BVDV 120 339
PCR - BVDV fluid 379 255 368 150 89
PCR - BVDV genotyping 9 5 6 1 2
PCR - BVDV tissue/blood 331 229 60 363 146
PCR - Calf diarrhea panel 470 541 637 701 759
PCR - Canine distemper virus 4 9 3 22 9 17
PCR - Caprine herpesvirus 1 2 3 PCR - Clostridium perfringens
genotyping 65 218 183 161 141 127
PCR - CSFV surveillance 2,170 443 1,214 1,155 1,119 1,383
PCR - E. coli genotyping 226 881 731 618 645 679
PCR - Equine adenovirus 1 1
PCR - Equine arteritis virus 4 10
PCR - Equine herpesvirus 1 and 4 6 36
PCR - Equine herpesvirus 2 4
PCR - Equine influenza virus 1 1
PCR - Erysipelothrix 12 67 94
PCR - FMD surveillance 2 4
32 ISU Veterinary Diagnostic Laboratory
Molecular Trends 2007 2008 2009 2010 2011 2012
PCR - Haemophilus parasuis 110 267 475 584 884 1,161 PCR - IBR virus (bovine herpesvirus
type 1) 34 169 174 157
PCR Influenza totals 7,635 6,785 5,850 6,576 15,856 24,783 PCR - Influenza A - Feral swine
surveillance 13 PCR - Influenza A - NAHLN
surveillance 42 48 1,498 1,573
PCR - Influenza A general 7,718
PCR - Influenza A NP gene 311 1,512 1,606 2,037 PCR - Influenza A screen and
subtype 6,581 1,570 4,464 9,721 6,424
PCR - Influenza A subtype 2,593
PCR - Influenza A surv subtype 168
PCR - Influenza HA gene 8 157 287 556 PCR - Influenza HA/NA gene
subtyping 1 46 332 321
PCR - Influenza M gene differential 225 322 21 8 PCR - Influenza pH1N1 differential -
Feral swine surveillance 2 PCR - Influenza pH1N1 differential -
NAHLN assay 1,945 3,000 PCR - Influenza pH1N1 differential -
NAHLN surveillance 9 27 431 385
PCR - Influenza SIV 7,635 3,547
PCR - Influenza SIV HA gene 204 137
PCR - Lawsonia intracellularis 173 384 812 1,052 1,777 1,849
PCR - Leptospira 271 507 372 367 565 472
PCR - Leptospira hardjo-bovis 319 60 11 43 26 1
PCR – M. bovis 79 386 399 387 418 320
PCR - M. flocculare 172
PCR - M. hyopneumoniae 2,422 4,339 6,344 4,472 6,303 1,990 PCR - M. hyopneumoniae Applied
Biosystems 3,951 PCR - M. hyopneumoniae
(mhp165/mhp183) 4
PCR - M. hyorhinis 108 774 906 1,705
PCR - M. hyosynoviae 116 377 716 1,251
PCR - Malignant Catarrhal Fever virus 1 8 6 PCR - Mycobacterium paratuberculosis
(Johne’s) 315 395 284 561 843 PCR - Mycobacterium paratuberculosis
(Johne’s) pool 25 46 137 26
PCR - P. multocida toxin 18 45 34 5 23 25
PCR - PCV fluid 59
PCR - PCV ORF2 27 58 3 15 11
PCR - PCV tissue 207
PCR - PCV2 3,559
PCR - PCV2 fluid 6,315 5,170 3,424 4,022 6,701 6,081
PCR - PCV2 tissue 861 511 386 412 777
PCR - Porcine Cytomegalovirus 21 12
PCR - Porcine Parvovirus 246 269 262 603 1,435 1,827
PCR - Porcine Rotavirus 320 2,039 2,347
33 ISU Veterinary Diagnostic Laboratory
Molecular Trends 2007 2008 2009 2010 2011 2012
PCR - Porcine Teschovirus 47 66 55 49 47 70
PCR PRRS totals 57,400 56,682 56,602 66,385 93,058 112,731 PCR - PRRSV 31,861 34,953 31,571 19,938 3
PCR - PRRSV (HATS) 25,539 20,573 23,730 14,188 48 PCR - PRRSV (HATS) Applied
Biosystems 29,399 44,530
PCR - PRRSV (HATS) Tetra-Core 5,523
PCR - PRRSV Applied Biosystems 44,526 62,551
PCR - PRRSV ORF5 1,156 1,301 3,077 4,996 5,615
PCR - PRRSV Tetra-Core 29,182 8,563 35
PCR - Rabies 2 1 1 2 1
PCR - Salmonella enteritidis 46
PCR - Salmonella spp 36 5 31
PCR - TGE 98
PCR - TGE/PRCV 1,300 1,251 1,068 1,170 1,928 1,516
PCR - Tritrichomonas foetus 169 457 804 1,411 PCR - virulent Newcastle Disease virus
fusion gene 1 17
PCR - WNV 40 12 9 9 3 13
PCR - unspecified 738 493 814 652 283 287
Quantitation Totals 4,335 4,805 7,966 4,964 11,955 15,696 Quantitation - M. hyopneumoniae 1,284
Quantitation - Influenza A 290
Quantitation - PCV2 2,763 2,501 2,521 2,620 9,024 7,782
Quantitation - PRRSV 1,557 2,289 5,419 2,331 2,606 6,340
Quantitation - PRRSV Tetracore 325
Quantitation - unspecified 15 15 26 13
RFLP - PRRSV 2 1
Sequencing Totals 1,598 1,341 1,368 2,625 4,145 5,578 Sequencing - Influenza HA gene 126 8 79 130 242
Sequencing - Influenza NAHLN surv 309 948
Sequencing - SIV 171 5 92
Sequencing - PCV ORF2 4
Sequencing - PCV2 224 21 12 3 12 9
Sequencing - PRRSV 1,173 1,184 1,256 2,543 3,694 4,310
Sequencing - unspecified 30 1 69
34 ISU Veterinary Diagnostic Laboratory
Pathology Testing
Pathology Trends 2007 2008 2009 2010 2011 2012
Biopsy Hematoxylin and Eosin Slides 1,231 1,202 1,175 995 1,125 960
Hematoxylin and Eosin Slides 40,811 36,702 33,123 35,175 40,365 42,614
Immunohistochemistry total 78,197 66,471 47,498 40,913 44,206 41,918
IHC - Bovine 2,900 1,931 1,602 2,068 2,031 1,308
IHC - BVD PI Biopsy 55,215 50,495 34,688 29,794 33,993 33,208
IHC - Porcine 19,020 12,576 9,696 8,049 7,794 7,119
IHC - Unclassified 1,062 1,469 1,512 1,002 388 283
Necropsy 2,696 2,758 2,359 5,716 4,217 2,302
Other 6 2 3,197
Parasitology 316 252 364 489 1,833 878
Special Stains 843 476 223 448 907 634 Serology Testing
Serology trends 2007 2008 2009 2010 2011 2012
Anaplasma marginale CARD 1,383 332 22 3 3 2
Anaplasma marginale cELISA 11,454 11,117 7,040 10,394 14,593 19,469
Anaplasma marginale CF 553 499 541 498 777 549
APP totals 8,469 41,610 17,999 9,133 43,311 63,326 APP 1-2-9-11 5,899
APP 3-6-8 172
APP 3-6-8-15 10
APP 3-6-8-15 5,999
APP 4-5-7 5,999
APP 10-12 569 1,334
APP ApxIV Elisa 705 233
APP CF screen (no serotyping) 5,667 22,146 10,228 5,401 19,544 21,762
APP CF serotype 1 only 11 5 5 108 46
APP CF serotype 3 only 2,569 18,688 7,522 3,641 18,139 20,768
APP CF serotype 5 only 11 48 6 109 46
APP CF serotype 7 only 81 3 4 109 46
APP CF serotype 157 130 15 1 15 16
APP Mix ELISA Screen 76 4,535 1,427
Avian pneumovirus ELISA 4,367 358 81 15 95 113
Baculovirus ELISA 1,300 8,766 5,474
Bluetongue virus AGID 1,024 589 503 950 1,112 205
Bluetongue virus cELISA 124 218 221 165 560
Bluetongue virus VMRD cELISA 818 1,633
Bovine leukemia virus AGID 15,205 13,539 8,578 13,102 17,172 21,075
Bovine leukemia virus ELISA 131 37 351 550
BRSV VN 586 443 173 800 405 180
Brucella totals 30,434 38,308 21,231 25,298 36,787 50,082 Brucella abortus FPA 621 4,124
Brucella BAPA SCREEN 19,608 25,798 12,864 16,900 19,195 24,283
35 ISU Veterinary Diagnostic Laboratory
Serology trends 2007 2008 2009 2010 2011 2012
Brucella canis RSAT 404 427 1,290 694 725 304
Brucella CARD test 4,388 6,413 4,244 5,507 8,192 4,966
Brucella RAP 2,845 212 75 4 50 2
Brucella RIV 214 206 33 56 83 51
Brucella SPT 745 638 655 691 775 1,060
Brucella STT 2,230 4,614 2,070 1,446 7,146 15,292
BVD antigen ELISA (PI) 5,535 3,231
BVD antigen ELISA (PI) Large EN 4
BVD antigen ELISA (PI) mod 238 77
BVD antigen ELISA (PI) Serum/EN 5,464
BVD type 1 VN 2,416 2,775 2,019 4,176 2,631 1,839
BVD type 2 VN 1,948 1,863 1,186 2,588 1,821 1,230
Canine herpesvirus VN 2 16 16 31 2
Canine influenza virus H3N8 HI 25 525 144 1
Caprine arthritis encephalitis AGID 387 149 185 199 43 8
Caprine arthritis encephalitis cELISA 534 564 478
Coxiella burnetii IFA 61
EHD virus AGID 611 574 469 525 733 688
EIA virus AGID 2,153 1,776 1,459 1,620 1,480 1,333
EIA virus ELISA 2,361 2,268 2,322 2,306 2,630 2,283
Hemophilus somnus CF 302 185 94 169 68 58
IBR (BHV-1) VN 1,649 2,029 1,211 1,703 1,894 1,397
Influenza totals 99,684 70,249 59,099 61,306 62,411 58,176 Avian influenza AGID 33,127 22,752 17,978 18,739 22,315 26,019
Avian influenza ELISA 481 926 573 626 200 790
Influenza Pandemic H1N1 virus 81 879 660 41
SIV H1 XP-012 (gamma) HI 2,398 1,499 1,856 1,488
SIV H1 XP-031 (delta) HI 528 256 306 120
SIV H1N1 (73) HI 8 106 114 41 3
SIV H1N1 (99) HI 13,193 8,006 5,946 6,789 4,689 3,560
SIV H1N1 Elisa 15,579 12,301 9,425 6,919 7,411 5,853
SIV H1N1 HI 20
SIV H1N1 HI (31) Pfizer 99 83
SIV H1N1 HI Pfizer 4,850 3,025 1,121
SIV H1N2 delta 1 Pfizer 3
SIV H1N2 Human like 2007013281 258 1,098 474 14
SIV H2N9 HI 6
SIV H3 XP-069 (Clus 4) HI 195 48 20
SIV H3N2 Elisa 8,659 8,078 7,158 5,571 6,098 5,069
SIV H3N2 HI 22,606 6,430
SIV H3N2 HI C1/3 7,035 4,965 1,182 7 126
SIV H3N2 HI C4 3,429 5,741 3,889 3,780
SIV H3N2 HI C4 2007013431 99 793
SIV HI Homologous 46 188 14 29
SIV NP Elisa 968 1,490 4,069 11,698 14,397 11,261
SIV NS1 Elisa 155
Johne's totals 27,009 23,631 15,731 16,942 19,692 24,310 Johne’s (bovine) CF 521 486 410 442 536 548
36 ISU Veterinary Diagnostic Laboratory
Serology trends 2007 2008 2009 2010 2011 2012
Johne’s (bovine) ELISA 25,938 23,092 15,228 16,458
Johne’s (ovine) AGID 550 53 93 42 105 61
Johne's ELISA - milk 68 92
Johne's ELISA - serum/plasma 18,983 23,609
Lawsonia intracellularis ELISA 661 990 1,682 1,344
Leptospira 5 MAT 2,679 3,093 1,667 1,973 2,233 2,407
Leptospira 6 MAT 3,466 3,765 2,309 1,833 1,476 1,147
Leptospira australis MAT 288 355 276 317 318 301
Leptospira autumnalis MAT 28 90 54 28 45 36
Leptospira ballum MAT 288 355 276 316 318 301
Leptospira bratislava MAT 107 364 35 28
Leptospira canicola MAT 63 108 212
Leptospira grippotyphosa MAT 1
Leptospira hardjo MAT 647 593 25 442 155
Leptospira ictero MAT 18
Leptospira pomona MAT 5 25 55
Leptospira sejroe MAT 291 739 276 316 318 301
Leptospira tarassovi MAT 288 355 276 316 318 301
M hyopneumoniae totals 32,112 44,062 43,461 45,931 50,880 47,160 M hyopneumoniae CF 166
M hyopneumoniae DAKO ELISA 2,950 12,008 12,663 5,027 3,711 1,954
M hyopneumoniae ELISA 29,162 27,637 22,752 31,165 36,754 31,053
M hyopneumoniae Tween 20 4,251 8,046 9,739 10,415 14,153
Neospora caninum ELISA 11,769 10,040 5,230 57
Neospora caninum VMRD cELISA 37 8,458 11,803 15,515
Ovine progressive pneumonia AGID 60 31 72 75 38 49
Pandemic H1N1 virus 41
Parainfluenza 3 Virus VN 602 358 95 460 281 388
Porcine circovirus totals 42,684 39,166 24,490 15,195 16,184 22,245 PCV 2 - 4 dilution 4,100 4,051 4,935 4,921 8,745
PCV 2 - cELISA 36,522 9,376 12,327
PCV 2 - Differential ELISA 12,453 8,529 5
PCV 2 - ELISA 10,043 10,964 8,807 9,339
PCV 2 - FFN 605 242 787 90 1,692 699
PCV 2 - IFA endpoint titration 183 81 1,358 232 474
PCV 2 - IFA screen 3,574 934 78
PCV 2 - IFA titer 1,557 1,787
PCV 2 - IgG Elisa 48
PCV 2 - IgM Elisa 426
PPV HI 3,989 3,000 2,644 1,625 2,712 2,012
PRRS totals 190,004 247,029 126,723 132,539 149,089 157,561 PRRS ELISA 157,031 146,489 119,150 33,588 72 PRRS ELISA (meat juice
surveillance) 27,993 96,770
PRRS FFN 444 325 181 6 9 395 PRRS Homologous IFA North
American 240 PRRS Homologous IFA titration
North American 4
37 ISU Veterinary Diagnostic Laboratory
Serology trends 2007 2008 2009 2010 2011 2012
PRRS IFA 2,919 606
PRRS IFA European 1,616 1,379 2,491 2,906 1,509 1,191
PRRS IFA North American 1,456 3,015 3,828 2,811 1,756
PRRS IFA North American titration 3 3 1
PRRS IFA titration 1 1
PRRS Oral Fluid Antibody 1,883 1,922 444
PRRSX3 ELISA 90,045 143,115 146,560
PRRSX3 Oral Fluid Antibody 1,129 7,658
PRV totals 184,628 92,193 32,628 33,179 43,752 34,436 PRV ALA SCREEN 1,773 2,114 1,978 1,466 1,127 8
PRV ELISA SCREEN
PRV g1 ELISA 178,017 83,115 23,663 15,508 16,515 2,159
PRV gB ELISA SCREEN 1,900 1,467 2,269 2,418 6,463 17,380 PRV gB ELISA SCREEN NAHLN
surveillance 9,631 10,347 9,716
PRV VN 2,938 5,497 4,718 4,156 9,300 5,173
Pullorum - typhoid AGG 32 333 1 5 56
TGE virus VN 2,213 7,475 3,335 1,399 6,749 14,644
TGE/PRCV 14,968 13,530
TGE/PRCV Biovet 32 16
TGE/PRCV Heated 8,976 10,349 11,467 12,630
Toxoplasma gondii ELISA 74,659
Toxoplasma LAT 420 235 548 992 615 573
Trichinella spiralis ELISA 74,708
VS virus Indiana strain VN 1,951 1,442 1,834 3,017 2,132 1,590
VS virus New Jersey strain VN 1,950 1,442 1,835 2,996 2,132 1,590
West Nile Virus Capture IgM ELISA 69 43 56 30 76 *BVD PI previously tested in Virology Toxicology/Nutrition Testing
Toxicology/Nutritinon testing trends 2007 2008 2009 2010 2011 2012
Ammonia Nitrogen 21 15 19 12 19 21
Antibiotics - Ampicillin 1
Antibiotics - Chlortetracycline 140 83 93 78 115 31
Antibiotics - Lincomycin 2
Antibiotics - Oxytetracycline 6 6 2 5 2 15
Antibiotics - Penicillin 6 3 18 8 3 3
Antibiotics - Tetracycline 4 15 13 122 7 15
Antibiotics - Tylosin 130 81 75 70 52 13
Antibiotics - Virginiamycin 8
Anticoagulant - Brodifacoum 1 6
Anticoagulant - Bromodiolone 3
38 ISU Veterinary Diagnostic Laboratory
Toxicology/Nutritinon testing trends 2007 2008 2009 2010 2011 2012
Anticoagulant - Dicoumarol 1 1 1 1 5
Anticoagulant - Diphacinone 2 4
Anticoagulant - Warfarin 1
Anticoagulant Panel 18 14 9 19 20 23
Arsenic in feed or tissue 7 16 12 18 10 16
Arsenic in fluids 7 2 1
Ash 13 11 1 15
Barbiturates
Bone Ash 22 16 8 73 309 332
Bone Density 19 12 4 69 250 306
Bone Profile 8 98
Cadmium in feed or tissue 4 10 18
Cadmium in fluids 6
Calcium in feed or tissue 26 13 10 30 53
Calcium in serum, plasma, eye, water 1 3 2
Carbamate 1 1
Carbamate - quantitation 5 3
Carbamate Panel 14 23 14 7 6 1
Carboxyhemoglobin 2 5 5 6 13 10
Chloride in feed or tissue
Chloride in fluids 7 2
Chlorinated Hydrocarbon Panel 3 2 2 3 1
Cholinesterase 34 91 65 35 48
Cholinesterase in blood 15
Cholinesterase in brain 12
CNS Canine Panel 2 2
Copper in blood
Copper in Feed or Tissue 185 156 167 32 61 48
Copper in liver biopsy 11 4 4
Copper in serum or water 132 27 23 19 16 3
Coumestrol - quantitation 1 3
Custom Chemistry 92 131 134 149 623 576
Cyanide 2 3 5 2 3 10
Ergopeptine Panel 12 6 9 1 4 28
Ergotamine 1
Ergovaline 35 46
Ethylene Glycol 17 5 3 3 1
Extraction: Panel 24
Extraction: Single Compound 28
Feed Particle Size 5 4 6
39 ISU Veterinary Diagnostic Laboratory
Toxicology/Nutritinon testing trends 2007 2008 2009 2010 2011 2012
Fluoride 1 18 20 4 5
Fluoride in tissue 2 2
Fumonisins LC/MS/MS Screen 4
GCMS Screen 4 38 28
GCMS Single Compound 3 2
Gossypol 5 1 2 2 2
Gossypol - quantitation 2 1 1 2 5
Heavy Metals Panel for feed or tissue 42
Heavy Metals Panel for fluid 1 Hormonally active feed compounds
panel 2
Hormone Free Feed Panel 11
Ionophores Screen 3 43
Iron in feed or tissue 28 26 20 3 17 26
Iron in liver biopsy
Iron in serum or water 48 14 10 10 16 1
Lasalocid 15 16 14 2 6
Lasalocid 1ppm LOD 1 49
Lead in blood or water 55 44 57 30 414 163
Lead in feed or tissue 36 36 64 44 96 61
Magnesium in feed or tissue 1 2 1 6
Magnesium in fluids 1 3
Manganese in feed or tissue 92 75 11 7
Manganese in serum or water 14 2 2
Melengesterol Acetate 8 5 12 9 3 1
Mercury in blood 1 2 1 1
Mercury in feed or tissue 6 2 3 8 10
Methemaglobin 28 5
Microtox Test
Moisture 71 94 34 22 36 232
Molybdenum in feed or tissue 4 3 1
Molybdenum in fluids 7
Monensin 76 34 20 19 17
Monensin - quantitation 22 17 8 3
Monensin 1ppm LOD 1 67
Mycotoxin - individual - quantitation 21 32 90 31
Mycotoxin - Aflatoxin - qualitative 6 11 10 7 1 Mycotoxin - Aflatoxin - qualitative M1
Liver 4 1 3 11 7 21
Mycotoxin - Aflatoxins ELISA 43 33 Mycotoxin - Aflatoxins LC/MS/MS
Screen 53
40 ISU Veterinary Diagnostic Laboratory
Toxicology/Nutritinon testing trends 2007 2008 2009 2010 2011 2012
Mycotoxin - Fumonisin B1 - qualitative 12 13 3 1
Mycotoxin - Fumonisins ELISA 1 5
Mycotoxin - Ochratoxin - qualitative 1 6
Mycotoxin - T-2 toxin - qualitative 8
Mycotoxin - Tremorgen panel 2 2 5 8 6
Mycotoxin - Vomitoxin - qualitative 2 25 29 17
Mycotoxin - Vomitoxin ELISA 3 18
Mycotoxin - Vomitoxin LC/MS/MS 3
Mycotoxin - Zearalenone ELISA 1 10 1 Mycotoxin - Zearalenone LC/MS/MS
Screen 1 Mycotoxin - Zearalenone/Zearalenol -
qualitative 3 13 14 4 5
Mycotoxin DDGS Panel 2 10
Mycotoxin ELISA Screen 31 Mycotoxin ELISA Screen 1 - includes 2
mycotoxins 1 7 2 Mycotoxin ELISA Screen 2 - includes 3
mycotoxins 30 2 2 Mycotoxin ELISA Screen 3 - includes 4
mycotoxins 123 183 9 15
Mycotoxin Panel 132 73 153 61 23
Mycotoxins LC/MS/MS Screen 98
Nitrate/Nitrite in feed 182
Nitrate/Nitrite in fluids 1 181
Nitrates in feed 8 18 4 5 29
Nitrates in serum or eye fluid 43 27 36 25 136
Nitrite analysis 3
Nitrite or Nitrate in water 11 13 11 8
Organophosphate - qualitative 2 1 3 4 4
Organophosphate - quantitation 5 2 2 6 2
Organophosphate Panel 19 24 10 10 5 1
pH 8 12 20 13 15 31
Phosphorus in feed or tissue 16 12 8 33 53 35
Potassium in feed 1
Potassium in fluids
Selenium GLP Method 5
Selenium in blood, serum or plasma 214 149 79 93 318 229
Selenium in tissue or feed 303 316 199 112 87 704
Slaframine 2 2 6 3
Sodium in feed or tissue 14 41 11 16 31 17
Sodium in fluids 2 6 9
Strychnine 8 1 2 2 1
41 ISU Veterinary Diagnostic Laboratory
Toxicology/Nutritinon testing trends 2007 2008 2009 2010 2011 2012
Sulfa Drug Panel 1 4 1
Sulfate in feed 63 65 27 18 40 27
Sulfate in water 27 28 20 10 13 16
Sulfonamide - qualitative 3
Total Dissolve Solids 3 2 1
Trace mineral panel - bovine liver 66 255 197
Trace mineral panel - bovine serum 50 186 151
Trace mineral panel - feed 64 53 40 118
Trace mineral panel - porcine liver 55 221 282
Trace mineral panel - porcine serum 52 80
Trace mineral panel - serum, water 131 221 27 63
Trace mineral panel - tissue 287 376 50 92
Urea 10 1 5 1
V9 mineral panel 58 29 12
Vitamin A acetate 1 18 16
Vitamin A in serum 14 9 14 59 218 533 Vitamin A palmitate in feed -
quantitation 4 2
Vitamin A palmitate in liver - quantitation 14 4 6 11 48 91
Vitamin D3 in feed 1 6 4
Vitamin E acetate in feed - quantitation 3 11 25 8 2 19
Vitamin E in serum 289 71 66 400 780 1259
Vitamin E in tissue 235 175 325 264 280 304
Water Quality Panel 42 35 55 40 54 67
Zinc in feed or tissue 30 31 9 3 15
Zinc in liver biopsy
Zinc in serum or urine 17 10 7 4 8 4
2012 Toxicology diagnosis Avian Bovine Canine Caprine Equine Non-
Animal Ovine Porcine Grand
Total
Water deprivation 8 8
Lead 1 6 7
Copper 1 3 4
Ethylene glycol (antifreeze) 4 4
Ionophore 4 4
Vitamin D 4 4
Idiopathic 1 2 3
Nephropathy,Undetermined 1 1 2
Ammonia 2 2
Nitrate 2 2
Hepatitis 1 1
Myopathy, Toxic, Misc 1 1
42 ISU Veterinary Diagnostic Laboratory
2012 Toxicology diagnosis Avian Bovine Canine Caprine Equine Non-
Animal Ovine Porcine Grand
Total
Algae 1 1
Diphacinone 1 1
Monensin 1 1
Organophosphate 1 1
Oxalate 1 1 Urea 1 1
Vitamins 1 1
Japanese yew (Taxus sp) 1 1
Zinc 1 1 Virology Testing
Virology trends 2007 2008 2009 2010 2011 2012
Antigen ELISA total 5,970 6,090 4,684 1,578 24 14
AgELISA - BVD virus 3,824 3,831 3,237 1,110 3
AgELISA - BVD virus modified 146 156 163 29
AgELISA - Influenza A virus 4 6 4 6
AgELISA - Parvovirus 13 11 13 4 15 7
AgELISA - Type A Rotavirus 1,983 2,086 1,267 429 6 7
AgELISA - unspecified
Fluorescent antibody total 740 556 469 317 306 257
FA - Border disease virus 3
FA - Bovine adenovirus 2
FA - Bovine coronavirus 2
FA - Bovine herpesvirus type 1 71 87 51 13 3
FA - Bovine parainfluenza virus type 3 10 6 3 6 1
FA - Bovine respiratory syncytial virus 4 10
FA - Bovine viral diarrhea virus 4 10 1
FA - Canine adenovirus 19 29 18 11 23 13
FA - Canine coronavirus 7 12 20 17 5 2
FA - Canine distemper 57 65 39 39 42 22
FA - Canine herpesvirus 44 46 39 24 39 30
FA - Canine parvovirus 64 76 98 91 50 43
FA - Encephalomyocarditis virus 1 5 4 1 5
FA - Equine herpesvirus type 1&4 27 21 18 11 11 3
FA - Equine herpesvirus type 3 13 1 1
FA - Feline calicivirus 21 15 7 6 8 3
FA - Feline coronavirus 1 1 1 2
FA - Feline herpesvirus 19 15 8 7 9 3
FA - Feline infectious peritonitis virus 22 7 3 2 2
FA - Feline parvovirus 19 12 8 4 8 7 FA - Hemagglutinating encephalomyelitis
virus 3 18 11 11 5
43 ISU Veterinary Diagnostic Laboratory
FA - Porcine enterovirus & teschovirus 12
FA - Porcine parvovirus 41 19 21 23 1 46
FA - Porcine respiratory coronavirus 1
FA - Pseudorabies virus 58 38 79 8 33 17
FA - TGE 12 2 3 1
FA - unspecified 234 76 28 40 51 40
Rabies 1,031 1,049 997 822 783 842
RFLP 4 4
Virus isolation total 1,254 1,365 905 1,058 2,269 2,264
VI - Avian influenza virus 100 258 98
VI - Border disease 1
VI - Bovine parainfluenza virus type 3 1
VI - Bovine viral diarrhea virus 157 383 140 156 262 510
VI - Canine adenovirus 2
VI - Canine distemper virus 2
VI - Canine herpesvirus 3 7 4 2 1
VI - Canine parvovirus 4
VI - Encephalomyocarditis virus 2 7 6
VI - Equine herpesvirus type 1&4 9 5 7 6
VI - Equine influenza virus 1 2
VI - Feline calicivirus 1 3 1 1 6 2
VI - Feline herpesvirus 1 3 2 2 7 2
VI - Feline parvovirus 1 1
VI - Feral swine influenza surveillance 2
VI - IBR virus (BHV-1) 59 177 49 5 3 5
VI - Porcine circovirus type 1 1
VI - Porcine circovirus type 2 117 12 15 2 7 21
VI - Porcine enterovirus 23 14 19 7 2
VI - Porcine parvovirus 1 1 1
VI - Porcine respiratory coronavirus 3
VI - PRRS virus 317 258 307 333 588 419
VI - Pseudorabies virus 32 42 75 7
VI - Swine influenza NAHLN surveillance 434 529
VI - Swine influenza virus 409 388 249 360 645 665
VI - unspecified 121 58 28 80 48 8
Virus propagation 5 13 10 1 1
Virus quantitation 1 7
Virus return 224 181 122 148 331 424
44 ISU Veterinary Diagnostic Laboratory
Rabies
2012 Rabies Accessions by month Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Total
Grand Total 52 41 65 66 101 71 82 130 53 59 65 57 842
Bat 12 10 7 9 25 15 25 77 14 10 13 18 235
Feline 11 7 16 15 17 21 20 21 8 14 13 14 177
Canine 15 8 16 16 18 14 18 15 11 12 15 11 169
Bovine 6 6 11 15 19 5 3 5 3 5 8 2 88
Raccoon 5 8 8 4 9 5 2 2 9 6 9 6 73
Skunks 1 2 2 1 2 5 8 2 2 4 29
Rodent 1 1 4 5 1 3 4 2 3 24
Equine 3 2 3 1 2 1 3 4 2 21
Ovine 1 2 1 1 2 7
Caprine 1 2 1 1 1 6
Marsupial 1 1 3 5
Cervidae 2 2
Bovine Exotic 1 1
Camelid 1 1
Canine Exotic 1 1
Mustelid 1 1
Ovine Exotic 1 1
Porcine Exotic 1 1
2012 Rabies accessions Positive Negative Inconclusive Unsuitable Total
Grand Total 25 781 28 8 842 Bat 11 224 235
Feline 175 2 177 Canine 159 8 2 169
Bovine 5 71 11 1 88 Procyonidae 67 4 2 73
Mephitidae 9 18 1 1 29 Rodent 24 24
Equine 20 1 21
Ovine 6 1 7 Caprine 6 6
Marsupial 4 1 5 Cervidae 2 2
Bovine Exotic 1 1 Camelid 1 1
Canine Exotic 1 1 Mustelid 1 1
Ovine Exotic 1 1 Porcine Exotic 1 1
45 ISU Veterinary Diagnostic Laboratory
Rabies Positive Trends 2005 2006 2007 2008 2009 2010 2011 2012
Total rabies submissions 1095 975 1031 1049 997 822 783 842 Total positives 101 41 22 17 29 20 19 25
% positive 9% 4% 2% 1.60% 3% 2% 2% 3%
Positive animals Bat 43 19 7 6 6 7 7 11 Skunk 29 12 4 4 12 10 7 9 Bovine 6 2 1 4 1 4 5 Feline 21 5 6 5 4 1 1 Canine 2 2 4 1 2 1 Equine 1 1 1
0
20
40
60
80
100
120
140
Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec
2012 Rabies Submissions to ISU VDL
Cases per month
0
200
400
600
800
1000
1200
2005 2006 2007 2008 2009 2010 2011 2012
Rabies Submissions to ISU VDL
Total number of rabies cases
Number of positive cases
46 ISU Veterinary Diagnostic Laboratory
Glossary
AgELISA Antigen-capture ELISA AGG Agglutination AGID Agar gel immunodiffusion AIV Avian influenza virus ALA Automated latex agglutination BAPA Buffered acidified plate antigen test BRSV Bovine respiratory syncytial virus BSE Bovine spongiform encephalopathy BVDV Bovine viral diarrhea virus cELISA Competitive (blocking) ELISA CF Complement fixation CSF Classical Swine Fever ELISA Enzyme-linked immunosorbent assay FA Fluorescent antibody FAD Foreign Animal Diseases FATS Fluorescent antibody tissue section FFN Fluorescent focus neutralization HI Hemagglutination inhibition IFA Indirect fluorescent antibody IHC Immunohistochemistry IPT Immunoperoxidase test LAT Latex agglutination MAT Microscopic agglutination test NADC National Animal Disease Center (USDA:ARS) NVSL National Veterinary Services Laboratories (USDA:APHIS) PCFIA Particle concentration fluorescence immunoassay PCR Polymerase chain reaction PCV Porcine circovirus PI Persistent infection PPV Porcine parvovirus PRCV Porcine respiratory coronavirus PRRS virus Porcine reproductive and respiratory syndrome virus PRV Pseudorabies (Aujeszky’s disease) virus RAP Rapid automated presumptive test RFLP Restriction fragment length polymorphism RIV Rivanol precipitation – plate agglutination test RSAT Rapid slide agglutination test SIV Swine influenza virus SPT Standard plate agglutination test STT Standard tube agglutination test TGEV Transmissible gastroenteritis virus VDL Veterinary Diagnostic Laboratory, Iowa State University VDPAM Department of Veterinary Diagnostic and Production Animal Medicine VI Virus isolation VN Serum-virus neutralization