Using a Compound Light Microscope

And Observing Live Protists

how you found sizes of microorganisms

• If the field diameter at 400 X is 450 µm,• How big is one of the following cells?

• HINT: Divide 450 µm• how many across

Human Skin Cells 400XNucleus plasma membrane cytoplasm

Onion Cells400 X

nucleus

Cell wall

Potato Cells400 X

• Dyed with iodine

Starch crystals

Elodea Cells

• 100 X 400 Xchloroplasts

Elodea Cells

• In fresh water In salt water• Full water vacuole empty water vacuole

Cyclosis in Elodea

• Cyclosis in Elodea 1:09• Elodea cyclosis 1:56• Rapid cycling of cytoplasm• You can see the chloroplasts moving also

STENTOR

• Stentor youtube 2:36• Stentor Cilia 40 sec• Horn-shaped• Up to 2 mm long• Cilia to bring in food• Stretches

Blespharisma

• Blespharisma Youtube 2:49• Blespharisma 2:18• 150-300 µm long• Ciliate• Eat bacteria by drawing them in• Often pink

Paramecium

• 50- 330 µm Slipper-shaped• Cilia, can spiral move• HD paramecium 13:06• Paramecium eating dyed yeast (Congo Red) 1:59

Euglena

• 25-100 µm• Flagellate• Euglena youtube 14:46• Nice flagella movement 51 secs

Presence of flagella (animal-like)Chlorophyll (plant-like)Red spot (light sensitive)

Spirostomum

• Spirostomum youtube 50 secs• HD Spirostomum 1:40

1-3 mm longSpiral rows of ciliaCan be seen without a microscope

mouth