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Unravelling factors affecting Sclerotinia stem rot in canola particularly in the light of fungicide spray decisions Ravjit Khangura 25 Feb 2015

Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

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Page 1: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Unravelling factors affecting Sclerotinia stem rot in canola

particularly in the light of fungicide spray decisions

Ravjit Khangura25 Feb 2015

Page 2: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Outline

1. Sclerotinia levels

2. Epidemiology

3. 2014 Petal testing

4. Case studies

5. Key messages

Photo: Kith Jayasena, DAFWA

Page 3: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Sclerotinia Stem rot incidence in WA in 2014

• Widespread but not severe

• Few crops still suffered economic losses

• Early development of apothecia

• Less favourable conditions post flowering

• Sclerotinia caused an estimated loss of $23M

in 2014

Page 4: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Sclerotinia Life Cycle

• Insert Text here

Page 5: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Epidemiology of SSR: Pre-infection

• Spore trapping

• Apothecia monitoring

• Petal testing

Page 6: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Petal Testing

• 2010-2014

• 6 sites

• Daily temperature, rainfall and relative

humidity recorded

Page 7: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Petal testing – Key findings

• Timing and frequency of petal infection

depended on

– Rainfall*

– Relative humidity*

• Air and soil temperature not significant

Page 8: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Conditions prior to max petal infection

0

20

40

60

80

100

29-Jun 6-Jul

Ra

infa

ll (

mm

), m

ea

n a

ir

an

d s

oil

T (

⁰C),

RH

(%

)

Rain

mean T

mean soil T

mean RH

0

20

40

60

80

100

22-Jun 29-Jun 6-Jul

Ra

infa

ll (

mm

), a

ir a

nd

so

il

T (

C),

RH

(%

)

Rain

mean T

mean soil T

mean RH

Conducive year

Non conducive year

Page 9: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

0

20

40

60

80

100

27/07 3/08

Ra

infa

ll (

mm

), m

ea

n a

ir

an

d s

oil

T (°C

), R

H (

%)

rainfall

mean T

Soil meanT

mean RH

Conditions after max petal infection

0

20

40

60

80

100

27/08 3/09

(Ra

infa

ll (

mm

), m

ea

n a

ir

an

d s

oil

T (°C

), R

H (

%)

rainfall

mean T

Soil meanT

mean RH

Conducive year

Non conducive year

Page 10: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Mean air temperature (Chapman)

0

5

10

15

20

25

11-Jun 1-Jul 21-Jul 10-Aug 30-Aug 19-Sep 9-Oct

Tem

pe

ratu

re (

⁰C)

Mean T 2010

Mean T 2011

Mean T 2012

Mean T 2013

Mean T 2014

Page 11: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Large scale petal testing in 2014

• Southern/South Coastal region

– 10 shires

• >200 samples

– 10 varieties

• sclero selective medium

Page 12: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Mean Petal infection in different shires

0

10

20

30

40

50

60

70

Me

an

PP

I

a

cd

d

ab ab abc abc abc abc

bcd

Page 13: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Percent petal infection-TOS

0

10

20

30

40

50

60

70

80

90

100

16/04/2014 1/05/2014 16/05/2014 31/05/2014

% p

eta

l in

fect

ion

Tme of Sowing

Page 14: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Petal infection vs Stem infection 2014

0

10

20

30

40

50

60

70

0 20 40 60 80 100

Ste

m i

nfe

ctio

n

Petal infection (%)

From 20 of 200 paddock validated for stem infection

Page 15: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Case study 1: Moora

0

10

20

30

40

1700

1800

1900

2000

2100

Product A @

1.5L/ha

Product A @ 1

L/ha

Prosaro @

450ml/ha

Nil

Yield (Kg/ha)

Disease incidence

0

20

40

60

80

100

14/07/14 21/07/14 28/07/14 4/08/14 11/08/14 18/08/14

Ra

in (

mm

), m

ea

n R

H (

%),

PI

(%) Rain

mean RH

PI

Page 16: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Case study 2: Wagin

0

2

4

6

8

10

12

14

1700

1800

1900

2000

2100

2200

Product A @

1.5L/ha

Product A @ 1

L/ha

Prosaro @

450ml/ha

Sumisclex @

1L/ha

Nil

Yield (Kg/ha)

Disease incidence

Page 17: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Row spacing, Prosaro and Density

Treatment Disease

incidence

lsd Yield/h

a

S.E.

Prosaro @ 450ml/ha

Nil 24 12 2359 75

Treated @ 30% bloom 13* 2568*

Variety

Cobra 16* 4 2247 43

Hyola 404 21 2725*

Row spacing

22cm 18 4 2452 51

44cm 19.5 2520

Plant density

15 plants /m2 17.7 4 2418 41

30 plants /m2 19.7 2554*

0

10

20

30

40

50

60

70

80

90

100

3/07 18/07 2/08 17/08

Rainfall

mean air T

mean RH

Estimated ROI from using Prosaro $50/ha

Page 18: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Decision to spray

>40mm rain, >75% RH

Page 19: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Where to from here?

• Regional risk forecast

• Validation

• Yield loss model

• Sclerotinia e-guide

WA Sclerocast

Page 20: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Key Messages

– Breakthrough in understanding the triggers

– >40mm rain and >75% RH in the 3 week

period before and after early bloom

– Temperature doesn’t seem to be a limiting

factor

– WA’s first Sclerocast is on the way

Page 21: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Acknowledgements

• Grains Research and Development Corporation

• A. Van Burgel, M. Aberra, JP Collins, M. Salam, J.

Lemon, WJ MacLeod, C. Beard, A. Smith, J. Galloway

and R. Loughman

• Geraldton RSU, GSARI

• Ashton Gray (ConsultAg), D. Cameron (Farmanco),

Tim Scott (Agvivo)

• Several WA Canola Growers

Page 22: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Thank You

Page 23: Unravelling factors affecting Sclerotinia stem rot in ... Khangura Crop Updat… · Cobra 16 * 4 2247 43 Hyola 404 21 2725 * Row spacing 22cm 18 4 2452 51 44cm 19.5 2520 Plant density

Questions?

Ravjit Khangura089368 [email protected]