9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

CONTROLLED SYNTHESIS OF NANOPARTICLES FOR INHALATION EXPOSURE STUDIES

M. MIETTINEN1, V. SUONMAA1, U. TAPPER2, U. BACKMAN2, J. JOUTSENSAARI1, A. AUVINEN2 and J.K. JOKINIEMI1,2

1Fine Particle and Aerosol Technology Laboratory, Department of Environmental Science, University of Kuopio, P.O.Box 1627, FI-70211 Kuopio, Finland

2Fine Particles, Technical Research Centre of Finland, P.O. Box 1000, 02044 VTT, Espoo, Finland

NOSA Aerosol Symposium Helsingør, Denmark, 8.-9.11.2007

9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

BACKGROUND Nanohealth research consortium

University of Kuopio University of Helsinki Finnish Institute of Occupational Health

Objectives controlled production of engineered nanoparticles exposure assessment in occupational environments adverse effects of nanoparticles

airway irritation and changes in the respiratory patterns inflammatory responses of the lungs and pulmonary cells genotoxicity

9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

EXPERIMENTAL SET-UP flow reactor Ti(C3H7O)4 liquid precursor in bubbler

N2 carrier gas

Ti(C3H7O)4 → TiO2 + 4C3H6 + 2H2O

dilution with dry and humified air size distributions: SMPS (3071A DMA+3022A CPC)

gaseous byproducts: FTIR (GASMETTM)

mass concentration: filter samples (Teflon,Gelman) TEM, XRD, BET

heat

9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

Qd1

T2

Tb

CO Qb

PC

PC N2

COCOQr

PCEXH

SMPS

fabric filter

EX

PO

SU

RE

CH

AM

BE

R

EXH

FTIR

T

MFC

Qd2

12 slpm

p

TEMroom air

HEPA

Tb = bubbler temperature T2 = reactor temperature Qb = N2 flow through bubbler Qr = residence time N2 flow Qd = prim. dilution air flowQd2 = secondary dilution air flow

= heated line

PC = pressure controller

CO = critical orifice

EXH = exhaust

9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

RESULTS: REACTOR T AND RESIDENCE TIME

0.0E+00

5.0E+06

1.0E+07

1.5E+07

2.0E+07

2.5E+07

3.0E+07

3.5E+07

4.0E+07

4.5E+07

5.0E+07

10 100 1000

Dp (nm)

dN

/dlo

gD

p (

#/c

m3)

GMD = 128 nmGSD = 1.6

GMD = 153 nmGSD = 1.6

GMD = 91.4 nmGSD = 1.6

T2 = 800 °C

T2= 800 °CQr = 0.5 slpm

T2 = 500 °C, Qr = 1.8 slpm

9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

T2 = 500 °C, Qr = 1.8 slpm T2 = 800 °C, Qr = 0.5 slpm

9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

T2 = 800 °C, Qr = 0.5 slpm3 ,

0 10 20 30 40 50 60 70 80 90 100

H2O

C3H6

CO

CH4

O2vol %

ppm

ppm

ppm

RH(%)

23.2

14.7

4.1

19.4

46.7

T2 = 500 °C, Qr = 1.8 slpm3 ,)

0 10 20 30 40 50 60 70 80 90 100

H2O

C3H6

CO

NOx

O2vol %

ppm

ppm

ppm

RH(%)

4.5

38.4

2.1

18.3

40.1

9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

RESULTS: DILUTION RATIO

0.0E+00

1.0E+07

2.0E+07

3.0E+07

4.0E+07

5.0E+07

6.0E+07

7.0E+07

8.0E+07

1 10 100 1000Dp [nm]

dN

/dlo

gD p

[#

/cm

3 ]

GMD = 128 nmGSD = 1.6

Cm = 34 mg/m3

DR = 3

GMD = 112 nmGSD = 1.6

Cm = 26 mg/m3

DR = 4.5

GMD = 91.4GSD = 1.6

Cm = 13 mg/m3

DR = 9

9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

CONCLUSIONS AND FUTURE Highly controlled nanoparticle generator Adjustable and repeatable

Inhalation exposure experiments have just started

In future new materials: copper, cobalt

9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

ACKNOWLEDGEMENTS

This work was funded by the Academy of Finland (decision number 118114) in the FinNano

research program

9.11.2007

Mirella MiettinenM.Sc., Research Scientist

UNIVERSITY OF KUOPIO

THANK YOU

AND BON APPETIT!