new possibilitiesUltiMate 3000 XRS

Resolution Throughput Connectivity

Thermo Scientific Dionex UltiMate 3000 XRS Systems

Thermo Scientific Dionex UltiMate 3000XRS Systems

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UltiMate 3000 XRS Systems redefining performance

The Thermo Scientific Dionex UltiMate 3000 XRS systems are designed

to optimize performance of small particle UHPLC columns while providing

the capability to handle pressures up to 1250 bar and seamless LC/MS

integration.

High Sample Throughput The UltiMate™ 3000 XRS quaternary UHPLC systems provide the lowest gradient delay volume in the market. Enjoy shortest run times without compromising on flexibility and resolution. Choose between the ultra-low dispersion UltiMate 3000 XRS Autosampler with its 3 well plate capacity or the UltiMate 3000 XRS Open Autosampler to extend the capacity up to 12 well plates. Combine this power with our industry-leading choice of UHPLC-compatible detection technologies.

Get more quality data with fewer experiments.

The Thermo Scientific Dionex UltiMate 3000 XRS systems are

to optimize performance of small particle UHPLC columns wh

the capability to handle pressures up to 1250 bar and seamles

integration.

High Sample ThroughputThe UltiMate™ 3000 XRS quaternary UHPLCprovide the lowest gradient delay volume in Enjoy shortest run times without compromisflexibility and resolution. Choose between thdispersion UltiMate 3000 XRS Autosampler 3 well plate capacity or the UltiMate 3000 XRAutosampler to extend the capacity up to 12Combine this power with our industry-leadinof UHPLC-compatible detection technologie

Seamless MS Connectivity Combine the industry-leading mass spectrometry portfolio with the most innovative UHPLC systems.

Proprietary Thermo Scientific Dionex Viper fingertight fitting technology ensures reliable zero-dead-volume connections for maximum performance. Take advantage of additional sample information without compromising chromatographic resolution. The unique 45 nL UV-monitor is especially designed for dispersion-free UV detection in LC/MS.

Fully exploit the capabilities of the mass spectrometer and the UltiMate 3000 XRS front-end with single point MS softwarecontrol powered by DCMSLink.

Ultra-High Resolution ChromatographyUse long sub-2 μm particle columns for ultra-high resolution chromatography and take full advantage of the extended pressure range of up to 1250 bar. The dispersion optimized sys-tem fluidics ensure exceptional separation performance and peak shapes.

Benefit from the Thermo Scientific Dionex UltiMate 3000 Thermostat-ted Column Compartment which easily holds up to six 250 mm columns.

Experience unmatched retention time precision through perfect flow control and ultra-stable column temperatures up to 110 °C.

Outstanding Versatility to meet your DemandsWe offer the most complete choice of UHPLC instruments in the industry. No matter what your applications, the UltiMate 3000 UHPLC systems will always perfectly adapt.

Choose from our unique range of detection technologies (UV/vis, fluorescence, diode array, charged aerosol, electrochemical detec-tion) or our industry-leading mass spectrometry solutions. Simply select the best detector to get the information you need.

Ultimate ReliabilityThe UltiMate 3000 modules set a new standard of robustness and durability in UHPLC.

Innovative technology and features, such as predictive performance monitoring, ensure lowest cost of ownership and maximize return on investment.

Rely on your results through reproducible chromatography every time, everywhere.

|3Get better results for the most demanding complex samples

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Solvent delivery and sample handling precision without compromises

Ultra-fast aflatoxin analysis on an UltiMate 3000 XRS system using a 1.5 min linear gradient. The overlay shows four aflatoxin species (AFB1, AFB2, AFG1, AFG2) at different concentration levels using a TSQ Vantage™ triple stage quadrupole mass spectrometer.

LPG-3400XRS Pump

Overlay of two chromatograms (time and peak area normalized) using an UltiMate 3000 XRS system with dispersion-free 45 nL flow cell installed (red) and a high-end UHPLC system of Vendor A (black). The XRS system achieved up to 2.75 times more theoretical plates for maximum resolution.

Advanced UHPLC Solvent DeliveryThe Thermo Scientific Dionex UltiMate 3000 XRS pump is a unique quaternary UHPLC pump optimized for the highest performance and lowest gradient delay volume. With pressures up to 1250 bar (18,000 psi) this pump offers state-of-the-art performance for high-speed and ultra-high resolution applications.

It combines the flexibility of a quaternary pump with the fast gradient performance of a binary pump. The ultra compact pump head design with a gradient delay volume of just 60 μL supports ballistic gradients for ultra-high throughput.

Fast Applications with Ballistic GradientsThe unique XRS pumping technology provides true ballistic gradient capability, by maximizing the fluidic advantages for the lowest delay volume in a quaternary solvent delivery system.

Optimized Dispersion for Maximum ResolutionTo get the best possible results, even from the most demanding complex samples, the quaternary UltiMate 3000 XRS systems are highly optimized to provide maximum resolution. This is achieved through an ultra-low dispersion flow path combined with dedicated zero-dead-volume Viper™ capillaries in 100 μm i.d. and 65 μm i.d.

Analytical Column: Thermo Scientific Hypersil GOLD 1.9 μm 2.1 x 50 mmMobile Phases: A: Water + 5 mM NH4OAc + 1% AA B: MeoH + 5 mM NH4OAc + 1% AA

Flow Rate: 600 μL/minInjection Volume: 1 μLMass Spectrometer: Thermo Scientific TSQ Vantage

1.10 1.20 1.30 1.40 1.50 1.60 1.70 1.80

Time (min)

Rela

tive

Abun

danc

e

100

90

80

70

60

50

40

30

20

10

0

AFB1(2.3.104 cts)AFB2

(2.7.103 cts)AFG1(2.3.104 cts)

AFG2(1.5.103 cts)

Time % A % B

0.00 80.0 20.0

0.10 80.0 20.0

1.60 5.0 95.0

1.85 5.0 95.0

1.86 80.0 20.0

2.50 80.0 20.0

Column: Hypersil GOLD™ 1.0 x 100 mm, 1.9 μm Conditions: Isocratic, 50%ACNInjection Volume: 1.3 μL

Uracil

1 Acetanilide

2 Acetophenone

3 Propiophenone

UltiMate 3000 XRS System

High-End UHPLC System of Vendor A

1 2

3

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Extremely low carryover for reliable LC/MS and MS/MS performance. The example shows a carryover experiment using beclomethasone. The carryover was typically below the limit-of-detection (LOD) of 0.0004%.

Lowest Dispersion and Highest EfficiencyThe Thermo Scientific Dionex WPS-3000XRS autosampler is dedicated to the particular requirements of ultra-resolution chromatography. The optimized fluidics use zero-dead-volume Viper connections to ensure the lowest possible peak dispersion for the sharpest peaks and exploit the full potential of the most advanced UHPLC columns.

Outstanding injection precision and accuracy of nL to mL sample volumes

Near zero-carryover even without a needle wash

Proprietary injection-valve design for maximum durability at ultra-high pressures

High Throughput Sample ManagerThe Thermo Scientific Dionex OAS-3000XRS open autosampler offers high sample capacity and speed. Its proven design makes it the first choice for any high throughput laboratory.

Support of up to 12 well plates

Injection cycle times down to 20 seconds for fast applications

Open architecture for easy sample access and customization

WPS-3000XRS™ Autosampler

OAS-3000XRS™ Open Autosampler

Ultra-Low Carryover for Every InjectionUltra-sensitive mass spectrometers demand the lowest possible carryover. Featuring advanced cleaning options, all UltiMate 3000 XRS autosamplers are optimized for ultra-trace analysis without interferences from previous injections, even in the case of highly sticky compounds.

Injection of 7 μL Beclomethasone (5000 μg/mL in MeOH)

Carry over typically below LOD of 0.0004%

Beclomethasone

Beclomethasone

min

Post Blank

mAU

5000

4000

3000

2000

1000

0

2.6 2.7 2.8 2.9 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7

mAU

5

4

3

2

1

0

2.6 2.7 2.8 2.9 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7

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Powerful and unique detectors see more from your analytes

High Speed QuanfirmationThe Thermo Scientific Q Exactive benchtop LC-MS/MS offers the best identification and quantitation results in one single run. It combines high-performance quadrupole precursor selection with high-resolution, accurate mass (HR/AM) Orbitrap detection. Fast scan speeds and multiplexing capabilities complete a package of unrivaled versatility. Combine it with the UltiMate 3000 XRS system and enjoy the synergies of two professionals in ultra-high speed and resolution.

Get the most complete answers to your analytical challenges in the shortest time possible

UltiMate Confidence in QuantitationAn expert system in quantitation, such as the Thermo Scientific TSQ Vantage triple stage quadrupole mass spectrometer, needs the most reliable LC front-end available. Challenging MS tasks like timed SRM transitions require precise chromatography. With its excellent retention time stability and injection volume precision, the UltiMate 3000 XRS system is the ideal front-end for

maximum trust in your MS results, even at the very lowest levels of quantitation.

A Near Universal Detection TechnologyCharged Aerosol Detection is a unique detection technology for non- or semi-volatile compounds. The Thermo Scientific Dionex Corona ultra RS detector provides consistent response independent of analyte structure

and ionization efficiency. Charged aerosol detection opens new opportunities for method development, QA/QC, and bioanalytical chemistry.

Corona™ ultra RS™ Charged Aerosol Detector

Q Exactive™ Mass Spectrometer

TSQ Vantage Mass Spectrometer

Q Exactive™ Mass Spectrometer

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1 Uracil

2 Acetanilide

3 Acetophenone

4 Propiophenone

5 Butyrophenone

6 Benzophenone

With UV flow cell

Without UV flow cell

0.00

1

2

3

4

5 67

0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50

WVL:245 nm

Time (min)

mAU

200

100

0

Complementary Detection OptionsThe UltiMate 3000 detector family covers a wide range of detection technologies. This makes it convenient to choose the detector that best optimizes sensitivity, specificity, and precision for your application requirements.

Boost your SensitivityThe Thermo Scientific Dionex Coulochem III detector provides utmost sensitivity for electro-active analytes. Adapt it to your application demands with a wide range of coulometric, amperometric, and UHPLC-compatible cells.

Use electrochemical detection coupled to mass spectrometry for signal enhancement, on-line synthesis of drug metabolites, studying analyte stability and identification of reactive centers, exploring bioactivation and adduct formation, and on-line peptide and protein cleavage.

Ultra-low Dispersion UV-monitoring for LC/MSGet additional information without compromising chromatographic resolution. The unique 45 nL UV-monitor is especially designed for dispersion-free UV detection in LC/MS.

The Thermo Scientific Dionex Variable Wavelength Detector provides UV/VIS detection at a data collection rate of up to 200 Hz for optimal support of ultrafast UHPLC.

Chromatograms with and without 45 nL zero-dispersion UV flow cell. No decrease in plate count was found. The XRS system achieved up to 2.75 times more theoretical plates for maximum resolution.

With 12 different detectors the UltiMate 3000 detector family offers industry-leading versatility.

Coulochem™ III Electrochemical detector

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Column handling and connectivity reliable separation with confidence

Precise Thermostatting for Any ColumnThe Thermo Scientific Dionex Thermostatted Column Compartment uses large-area Peltier elements and a fan-based forced-air design to provide efficient cooling and heating. This ensures fast equilibration upon startup, after opening the front door, and after changing the set temperature.

Wide temperature range; 5 °C up to 110 °C for reliable separations

Holds up to 6 columns to facilitate automated method development Freely configurable switching valves for advanced chromatographic techniques

High Performance ColumnsThermo Scientific columns provide exceptionally high column efficiencies, symmetrical peaks, and low bleed required by modern HPLC and LC/MS methods. A whole range of columns from general purpose to unique specialty columns are available.

Ultrapure silica substrates

Unique and versatile bonding chemistries with various end-capping

Wide range of different column sizes

TCC-3000RS Column Compartment

1.9 μm Hypersil GOLD column shows excellent peak shapes for all analyte types

1.7 μm Thermo Scientific Syncronis columns for consistent, predictable separations, column after column, time after time

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The First Universal Fitting SystemThe Viper and Thermo Scientific Dionex nanoViper fitting systems do away with problems experienced with conventional fitting systems. They provide a perfect fit each time and ensure superior chromatographic performance.

Zero-dead-volume UHPLC fingertight fittings suitable for system pressures of up to 1250 bar

Compatible with virtually every type of valve and column hardware

Flexible stainless steel or fused silica capillaries

Dedicated low-dispersion Viper capillaries with 65 μm i.d. perfectly connect the column with all Thermo Scientific mass spectrometers.

Slipping capillaries cause deteriorated peak shapes (left). Viper capillaries provide robust performance at UHPLC pressures (right).

0 9 18

600

300

0

Seconds

mA

U

Acetanilide Acetophenone

Uracil

Injection #4

Injection #101

0 9 18

600

300

0

Seconds

Acetanilide Acetophenone

Uracil Injection #1

Injection #50

Injection #100

26166

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Intelligent Chromatography SoftwareThermo Scientific Dionex Chromeleon 7 is the next-generation chromatography data system that uses Operational Simplicity™ as its guiding design principle. Chromeleon™ 7

takes you from samples to results in the shortest time possible.

Innovative eWorkflows™ enable anyone to start chromatographic analyses and generate results with just a few selections. eWorkflows automatically create sequences containing the correct instrument conditions, data processing parameters, and

reporting templates. Samples are run automatically, processed immediately, and reported instantly–ensuring the shortest possible time from samples to results.

Streamlining all workflows delivering instant results

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Chromeleon’s automatic integration and dynamic data processing reduce the analyst’s effort and display the results immediately. The unique and innovative integration tools, Cobra™ peak detection wizard, and SmartPeaks™ integration assistant, enhance the user experience and make detection and integration easy and straightforward.

Software for Mass SpectrometryDCMSLink is a free, control-only software package, providing fully integrated single-point control of any Thermo Scientific LC system through Thermo Scientific Xcalibur and mass spectrometry control software from other leading manufacturers.

Learning new software is held to a minimum with the MS software as the single user interface. Using DCMSLink to connect your

instrument to various MS software packages, the full benefits of the UltiMate 3000 systems are ready to support your MS applications.

BR70107_E 06/12S

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