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Two States of the Array
B. Ding & N.C. Seeman, Science 314, 1583-1585 (2006).
Transitions in the Array
B. Ding & N.C. Seeman, Science 314, 1583-1585 (2006)..
Transitions in the Array (Zoom)
B. Ding & N.C. Seeman, Science 314, 1583-1585 (2006).
Programming an Array for Assembly
A. Carbone & N.C. Seeman, Proc. Nat. Acad. Sci. (USA) 99 12577-12582 (2002).
A Ribosome-Like Device:
A DNA Nanomechanical Translation Machine
Shiping Liao
A Drawing of the Device
Liao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.
Movable Sticky Ends
Movable Sticky Ends
Fixed Sticky Ends
Principles of Operation
I1 2 74
3 5PX1 JX22
6
II
III
V
IV
1 2 7
3 65
4
PX1 JX22
I II
III
IV
V
1 3DX3
1 2DX2
4 6DX4
4 7DX5
5 7DX7
5 6DX6
DX2 DX5
Liao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.
Possible Products
Liao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.
Relationship toTraditional Components
2 4 60 1I II
III
IV
VA B
3 75PX1 PX2
Message Codon Equivalent Message Codon Equivalent
Amino Acid EquivalenttRNA Equivalent
Liao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.
DX Molecules Correspond to Aminoacyl tRNA Molecules
Anticodon Equivalent
Sequence
NNN------------------------------------NNN
1. MS beads
2. Release (Cleave DS2)
3. Ligate
4. Denature
IVIII
VI II
A B
0 1 2 74
3 65PX1 JX22
IV
VI I I
PX1 JX22A B
0 1 2 74
3 65I I I
FailedAssemblies
B
1. MS beads2. Release (Cleave DS1)
3. Add DS2
Purification, Ligation & Analysis
Liao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.
Sequencing Result
*
All Combinations WorkLiao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.
Polymer Unit
Visualized Application
DX Molecule
Organized Polymers
DNA Walking Biped
Bill Sherman
Schematic of the Device and Sidewalk
Sherman, W.B. & Seeman, N.C. (2004), NanoLett. 4, 1203-1207.
