Tutorial Genes Hox

7/23/2019 Tutorial Genes Hox

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SITIO: HTTP://WWW.NCBI.NLM.NIH.GOV/

ational Center for Biotechnology Informatio

TUTORIAL HOX
http://www.ncbi.nlm.nih.gov/http://www.ncbi.nlm.nih.gov/http://www.ncbi.nlm.nih.gov/http://www.ncbi.nlm.nih.gov/http://www.ncbi.nlm.nih.gov/http://www.ncbi.nlm.nih.gov/http://www.ncbi.nlm.nih.gov/http://www.ncbi.nlm.nih.gov/http://www.ncbi.nlm.nih.gov/


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INTRODUCCIN

Todos los genes Hox codifcn !" #c$o"es$"nsc"i!cionles %&e $ienen &n do'inio de &ni(n lADN '&) si'il" deno'indo *o'eodo'inio +ls!"o$e,ns %&e con$ienen es$os do'inios se conocenco'o *o'eo!"o$e,ns-.


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3

GN/ HOX0 Cons$"&cci(n de !lnosco"!o"les


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MTODOLOG1A

I'!o"$nci

s!ecies co'!"" C"c$e",s$ics 2iol(gics

i'!o"$n$es de cd &n

3"oceso de 24s%&ed en el NCBI


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HOMO SAPIENS

567 8 N4'e"o de sec&encis del HOXD5


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20 CROMOSOMA


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NOMENCLATURA

9 8 CROMO/OMA

% 8 2":o l"go

;5 8 "egi(n c"o'os('icdel 2":o l"go


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COPIAR A WORD


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RNGLON/

Homo Sapiens

5 gccggcgg gggccgccc $$ggccggc cgcgcccg $ggccgcccc cgccgg$$

75 $$ggccc$ $$$cc$cc ggc$cc$cg cc$ggg$$g gggggcg c$cggg$g

595 ggggc$ggc gggcggcgc gcggcggc g$cc$gc$c gcc$c$gcc cggc$ccg$

5?5 c$ccggcccc ggcc$gcgcc c$cggg $ggggcccg cc$ggc$ cc$cc$gg

9@5 g$cg$g$c $gcgcgc gcggcgggg$ cggcggcgc g$gc$cgc$ $ggcccc ;5 g$$c$gccgc $ccgcgccc ggcccg$ggc $c$gcgccc gcc$$ccc$c $gggccgg

;75 cgcggcgcc $$cg$cgc$ g$c$gcccc$ ggccgccgcc cgccc$cgc c$$cgccccc

@95 ggccgccccc gcgcggccg$ ccg$ccgcc $ccggccgcg ccccg$cg cgcg$gcc

@?5 cc$gggggg gcc$cgc c$gg$gccgc cc$gccgcg gcgc$gggg gcgcggc$

@5 cggc$$cc$g ggg$ccgggc cggcg$cg c$$cccgggc g$gc$ggggc gggcggccg

75 cgcggcggg $c$ccg$cc c$cgccc c$cggccg$c $$c$cgggcg gcggc$c$$$

775 cc$cc$cgc ggccgg$gg $$cgcggc c$$cggcg cccggccc$$ $$ccggc$$g

695 $c$cgcg $cgccgcg gccccc$gg $gc$$$ccg ccgc$ccc cggccccgg 6?5 ccc$cccc g$ccg$c$ c$cccgcc$c cggcc$ccc$ gccgcc$$c gccg$$cg

?@5 g$gg$g g$gggg $gcc$c$ ggg$ c$cgccgg$ $ggggccgc

5 $gcccc$cc gcgcg$cc gccg$$$ cgcccg cc$gcg c$gg

75 gg$$$c$ $$c$g$ c$$c$cg gcccggcgc $cgg$g ccc$gc$$

595 gccc$g$ gccgcg $c$c$g g$$ccgc cgcgg$g cgg

5?5 gggcg ggggc$$c $ggcccggc c$$cc$g$g gc$cccc$cc c$$cccc$

55@5 c$c$ggc cccccc$ g$$$$c gccccggc gccc$$c$c g$cccg

595 gcc$$cg$g ggccgg$c$ $ggggccg c$g$ggc c$gcgg$ cccggcgc

5975 cccc$ccc$ $c$gc$$ gggc$cg $$$ggg$gg gg$gggg c$g

5;95 $ggg$$$ cc$$ggg $gg$c$ $$g$$ggc$ $ccgg$$cc gc$$$g

5;?5 $ccg$$$ $$gc$g$ c$$g$gcc c$$g$$$gg $$$g$$$g $g$c$$$c

5@@5 ggggg$ gcccgc$gc cgcccgc$ ccgc$gc$$ c$$$gcc$c c$$g$c$g

55 $gc$$cgc g$$gcgg$ ggcgcc$ $g$$gc$g g$$c$g$cg cc$c$g$g

575 $gc$cgg gccc$gcc cg$$$$$ c$gg$$$$ $$$ggc ggc$c$

5795 $$$cgc$ $$$$gg$g ccgc$ g$$ggg$c$ cc$$g$g$ gc$gc$gc

57?5 $$cg$g$c $$cc$$$g cc$ccg$$ $$$$$$$c$$ $gcg g$$$c$cc$

56@5 c$c$$g$gc c$gg$g$g $c$cgc $g$$$g$$ c$g$gc $cccg

5?5 $ggg$ gg$g$c $gc$$$ c$$gc$ $$$$c c$$g$g$


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Ho'oEs!iensENME9@5

gccggcgggggccgccc$$ggccggccgcgcccg$ggccgcccccgccgg$$$$ggccc$$$$cc$ccggc$cc$cgcc$ggg$$ggggggcgc$cggg$gggggc$ggcgggcggcgcgcggcggcg$cc$gc$cgcc$c$gcccggc$ccg$c$ccggccccggcc$gcgccc$cggg$ggggcccgcc$ggc$cc$cc$ggg$cg$g$c$gcgcgcgcggcgggg$cggcggcgcg$gc$cgc$$ggccccg$$c$gccgc$ccgcgcccggcccg$ggc$c$gcgcccgcc$$ccc$c$gggccggcgcggcgcc$$cg$cgc$g$c$gcccc$ggccgccgcccgccc$cgcc$$cgcccccggccgcccccgcgcggccg$ccg$ccgcc$ccggccgcgccccg$cgcgcg$gcccc$gg

gggggcc$cgcc$gg$gccgccc$gccgcggcgc$gggggcgcggc$cggc$$cc$gggg$ccgggccggcg$cgc$$cccgggcg$gc$ggggcgggcggccgcgcggcggg$c$ccg$ccc$cgcccc$cggccg$c$$c$cgggcggcggc$c$$$cc$cc$cgcggccgg$gg$$cgcggcc$$cggcgcccggccc$$$$ccggc$$g$c$cgcg$cgccgcggccccc$gg$gc$$$ccgccgc$ccccggccccggccc$ccccg$ccg$c$c$cccgcc$ccggcc$ccc$gccgcc$$cgccg$$cgg$gg$gg$gggg$gcc$c$gggcc$cgccgg$$ggggccgc$gcccc$ccgcgcg$ccgccg$$$cgcccgcc$gcgc$gggg$$$c$$$c$g$c$$c$cggcccggcgc$cgg$gccc$gc$$gccc$g$gccgcg$c$c$gg$$ccgccgcgg$gcgggggcgggggc$$c$ggcccggcc$$cc$g$ggc$cccc$ccc$$cccc$c$c$ggccccccc$g$$$$cgccccggcgccc$$c$cg$cccggcc$$cg$gggccgg$c$$ggggccgc$g$ggcc$gcgg$cccggcgccccc$ccc$$c$gc$$gggc$cg$$$ggg$gggg$ggggc$g$ggg$$$cc$$ggg$gg$c$$$g$$ggc$$ccgg$$ccgc$$$g$ccg$$$$$gc$g$c$$g$gccc$$g$$$gg$$$g$$$g$g$c$$$cggggg$gcccgc$gccgcccgc$ccgc$gc$$c$$$gcc$cc$$g$c$g$gc$$cgcg$$gcgg$ggcgcc$$g$$gc$gg$$c$g$cgcc$c$g$g$gc$cgggccc$gcccg$$$$$c$gg$$$$$$$ggcggc$c$$$$cgc$$$$$gg$gccgc$g$$ggg$c$cc$$g$g$gc$gc$gc$$cg$g$c$$cc$$$gcc$ccg$$$$$$$$$c$$$gcgg$$$c$cc$c$c$$g$gcc$gg$g$g$c$cgc$g$$$g$$c$g$gc$cccg$ggg$gg$g$c$gc$$$c$$gc$$$$$cc$$g$g$$$$cg$$g$$$$$$$$g$$$gc$$gc$gcc$$$g$gc$$$$g$c$gc$g$ggg$$$$$$$$g$$$$$g$c$gc

3ongoEXME;66796

gccg$gcggggggccgccc$$ggccggccgcgcccg$ggccgcccccgccgg$$$$ggccc$$$$cc$cccc$cc$cgcc$ggc$$ggggggcgc$cggg$gggggg$ggcgggcggcgcgcggcggcg$cc$gc$cgcc$c$gcccggc$ccg$c$ccggccccggcc$gcgccc$cgg$ggggccggcc$ggc$cc$cc$ggg$cg$g$cg$gcgcgcgcggcgggg$cggcggcgcg$gc$cgc$$ggccccg$$c$gccgc$cggcgcccggcccg$ggc$c$gcgcccgcc$$ccc$c$gggccggcgcggcgcc$$cg$cgc$g$c$gcccc$ggccgccgcccgccc$ccc$$cgcccccggccgcccccgcgcggccgccc$ccgcc$ccggccgcgccccg$gcccc$gggggggcc$cgcc$gg$gccgccc$gccgcggcgcggggggcgcggc$cggc$$cc$gggg$ccgggccggcg$cgc$$cccgggcg$gc$ggggcgggcggccgcgcggcggg$c$ccg$ccc$cgcccc$cggccg$c$$c$cgggcggcggc$c$$$cc$cc$cgcggccgg$gg$$cgcggcc$$cggcggcccggcccc$$$ccggcg$g$c$cgcgccgcgcggccccc$gg$gc$$$ccgccgc$ccccggccccggccc$ccccg$ccg$c$c$cccgcc$gcggcc$ccc$gccgcc$$cgccg$$cgg$gg$gg$gggg$gcc$c$gggcc$cgccgg$$ggggccgc$gcccc$ccgcgcg$ccgccg$$$cgcccgcc$gcgc$gggg$$$c$$$c$g$c$$c$cggcccggcgc$cgg$gccc$gc$$gccc$g$gccgcg$c$c$gg$$ccgccgcgg$gcgggggcgggggc$$c$ggcccggcc$$cc$g$ggc$cccc$ccc$$cccc$c$c$ggccccccccg$$$$cgccccggcgccc$$c$cg$cccggcc$$cg$gggccgggc$$ggggcggc$g$ggcc$gcgg$cccggcgccccccccccg$c$gc$$gggc$cg$$$gg$gggg$ggggc$g$ggg$$$cc$$ggg$gg$c$$$g$$ggc$$c$g$$$ccgc$$g$ccg$$$$$ggc$g$c$$g$gccc$$g$$$gg$$$g$$$g$g$c$$$cggggg$gcccgc$gccgcccgc$ccgc$gc$g$c$$$gcc$cc$$g$c$$gc$$c$cg$$gcgg$ggcgcc$$g$$gc$gg$$c$g$cgcc$c$g$g$gc$cgggccc$gcccg$$$$$c$gg$$$$$$$ggcggc$c$g$$$cgc$$$$$gg$gccgc$g$$ggg$c$cc$$g$g$gc$gc$gc$$c$g$c$$cc$$$gcc$ccg$$$$$$$$$$c$$$gcgg$$$c$cc$c$c$$g$gcc$gg$g$g$c$cgc$g$$$g$$c$g$gc$c$cg$ggg$gg$g$c$gc$$$c$$gc$$$$$cc$$g$g$$$$cg$$g$$$$$$$$$$$$gc$$gc$gcc$$$g$gc$$$$g$c$gc$g$ggg$$$$$$$gg$$$$$g$c$gc


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/TA/ /CUNCIA/ 3A/ARLA/ AL BLOC D

NOTA/ COMO / MU/TRA N L ARCHIVOCORR/3ONDINT. Asign" no'2"e l "c*io se "e%&e"i"iJ.

/T ARCHIVO D BLOC D NOTA/ / L


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El programa Clustal se puede trabajar en lnea o bajar el programa

a su computadora


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ALINACIN N CLU/TAL

Uso del !"og"' NCBI

N$ionl Cen$e" #o" Bio$ec*nolog)

In#o"'$ion


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/TA / LA VNTANA ABIRTA D CLU/TAL

BAKADO A LA COM3UTADORA.


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Cargar el archivo del block de notas con las

secuencias, como se muestra:


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L ARCHIVO D LA/ /CUNCIA/CARGADO / VR A/10


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RALIAR L ALINAMINTOCOM3LTO COMO / MU/TRA0


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AL DAR LA ORDN 3ARA L ALINAMINTOCOM3LTO A3ARCRN UNA VNTANA3ARA GUARDAR LO/ R/ULTADO/. L

3ROGRAMA GUARDAR UN ARCHIVO CON LMI/MO NOMBR DL 3ROGRAMA UNT3RO CON LA XTN/IN ALNP. DAR O.


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D/3UQ/ D /TO A3ARCR N LA 3ANTALLALA/ /CUNCIA/ ALINADA/ > Q/T MI/MOR/ULTADO / GRABA N L ARCHIVO


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AL ABRIR L ARCHIVO CON TRMINACIN ALNP N L BLOCD NOTA/ VRN LO /IGUINT +/ MU/TRA UNA 3ART-0

L &senci de s$e"isco indic en donde *)di#e"encis.

Los g&iones indicn inse"cioneseli'inciones lco'!"" ls sec&encis.


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Un ee'!lo de ""eglo de sec&encis lineds. /e!&ede *ce" con xcel.


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/i "eli:n &n BLA/T con l sec&enci HOXD5 de*&'no e"Jn el g"do de si'ili$&d con losgenes *o'(logos de o$"s es!ecies0


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DI/CU/ION/ > CONCLU/IN

C&Jles son ls si'ili$&des ) ls di#e"encisencon$"ds


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GN/ HOMBOX

Unie"sidd de /ono"

De!"$'en$o de Ines$igcionesCien$,fcs ) Tecnol(gics

Mnd&no /ldo"

TSlle: V,c$o"3e"l$ M"$inM)$o"en KosS


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BostaurusXenopus (Silurana) tropicalis


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SECUENCIA DE XENOPUS (SIN REGLONES YNMEROS)

No. de !!e"o Ge#$#%:NM&00'0'*

+,,++++,!,!++,,!!,!+!,+,!!++!!++++,!,!!!,++,+,,!!,!!,+,!!,,!,,+!++++,+,,+,+!!,,,,!!!!+,,!,+!!+!+!+!!+!,+!!,,+!+!!,,!!!,++!+,+++!+,!,!!!,,,,+!!+,,++++,+,+!!,++!,!!+,,+!!!!!++!,,!,!!!+,!,!!+!!,,,,+!,!!,+!+!!,+,+,!+!,,,++!!!!!!!!!,,!,+,,!+,,,!,,!,+!+!!!+,,!+!,,,,++,!,,!!,,++,+++!,+++++!!!,,!!!,,++!+,,!,+,,,!!!+++,++,!+,!,!!,!,+++!+!,++!,!++!,!,,++++!!!,++!,!,!+!!!,+,++!!!,++,,,,!!,+++++!!,,+!++,,!,!!!!,,!+!!,++!!,,!!!!!,+!!,!!!!++!+,!,+!!!!,+,!+!!+,,,+,,++,++,,++!!!!!,++,,,!,!,+,,++++,+!+,!!!!!!,+!!!+,+++!!,,!!!!!!!,+!,+!,!++++,,,!,,,,!+,,!,!!!+++!+!+!,!+,!+!!,!!!,!!!,!!+,!,!++,,+,!,++,,!!+,++!+,+!+++++++++++,!,,,+!!!,,!,!!!!!+,!!++!!+!,!!+,,!,+!+,!,!!!,,,!+,!,+,!,+!,+!+!+!,+,!+!!,!!++!+,,,+,,+!,!!+!,++!!,,,+!+,!+,!,!++!++,,!!!+,+!,,!,+!,+!!!!!+!++!!!,+++,+!,,,+!!,,,,!!+,,!,,+,+,,!!,,,,+,!+,,+,,,+,,,!+!!,,+!+!,!,!!,+!,!,,+!,,,!,,,+++,!,++,,+,,,+,+++!+!,++++,+++,,!

!+,!,!,!!,,!!,,,,,,!,++,,+,,+,+,,!!,+!!,+!,+,+!+!++!+,!!,+!+!!+,!,+,!,!,+,,+!!+,!,+!,,!!+,++,+++,!,+!!,,,!!,!!,,,,!+!,+,,,+!!,,,+,+,,,,+!,+,,!!!,+++,!,!+!,,,!,,!,+!,+!,+!,!,,!,+++!+,,,+,+,!,!+++!,,+!!,+++!+,,,,,,,,,!,+!,+,,,,!,,,,,,,,,!,,,,!,,+!,,+


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SECUENCIA DE $OS (SIN RENGLONES Y NMEROS)No. de !!e"o Ge#$#%: XM&002*-30

++++!++,!++!,+!++++++,+++!++!+!++!+!++!++,!!,+!++!!!!!!!!++!!!++!!++!+!+!,!!+

+,+++,+++!!++!!,++!,!!,!!,!+!,!+,+,!+,+!++!!+++!++!++!+!,,+!,++!,,!+!,!!+,,!,+,!+!+!!+!+!!!++!!!+,++,!!,+!+!!!+!!,,!!!!!,+++!+!++!+!++!+!+,,!+,,+!,+!!,+!!!!,++!!+!++!+!++!++,+!!!,!+!!!!!++!!+!!!!++!+!+!!,!!++++!!,+!!+!+!!!+!+,!+!+!!+,+!!!!!,++++++!!,!++!!++!+!!+!!!,+,!!++!!+++++!+++!,!++,!,!!!++++,!!+!+!!!+!+,!+,,,!!+,+!+!+!,!+++!++!!++!+!+!+!++++!+!!+,!!,,!+!!!!,!++!,+,!,,!,!,++++!++!,!!,,!!,!!,!+,++!!++,++,,!+!++!!,,!++!++!!+++!!!!,,,,!++!+,+,!,!++!!+!!+!++!!!,!,++++!!,,,!+!!+!+,!!!!!+!,!!!++!+,!,!!!!+,+,+!+,!!!!+!!,!!++!!,!!!,+!!+!!,,!+!!+,,!++,++,++,++++!+!!!!+++!,,!+!+++,,+++!!+!!!!!!!,!!+!+!+,!!+!!+,,,!+!!!+!!,+!+!,++++++,,,!,,,,,+,!,,!,!+++!!!+!+!,!++,+!!!,!,,+!!,+,+!!!!+,!,!,++,,!!+!!+!++,+

!+++++!++++++!,,!,+!!,!++!!!!!!!+,++!,,!!,,!!+!,,!!!,!,!++!++!!!,+!!+,!,++!+!!!+++++!!!!,!,!++!!!++!!,!!,+++,,,+,!!+,!+!,+,+!,+,+++!,+!+!,!,,+++!!!!!!!!!,,!,!,++!,,,,+!,!+,,,++,++++,+++!,++,++,++!+,,,!!,,++++++,!!,,++,++!,,!,++,,!!+++!,+,+++,+,!+,+,,,,,++,!,!,+!!!,!,!,+,+,,,+,+,!,,,!++++,+!!!+!!+!!+!!!+!!!,!,+!,,+,,+!!,!,!+,!,,+!+,,,,!!+,+!++,++!++!,,!!,,+!,!,+!!!!!!+,+,+,+!,!+++!,!,!!!+,,,,,!,++,,,,,,,+!++!,!,+,,,!+!,,!,+,+!!+!,+,,,!,,++!!,!!,,++,,,+!,+!,,!!!+,+,!!,!!,,,+!!,+,,,,,,,,!!!!!,,+!+,,,!,!!,!,!!++,+!!,+,++,!,!,!,+,,,+,,+,+!,!++!!+++,++,+!!!,+!,,!!,,+!!!+,,!,!!,,+,+,,,,!+,,+,,,,,,,++,,,+!,,+,+,+!,,!,,++!,,,,+,!+!,+,,++,,,+!,,,,,,,++,

,,+!+,+,+,!


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/ec&encis lineds


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,,://.e1.!.%/Too4"/"e56!e"/e1/,oo45e

"4,.e178o1Id9!4",42I20';''20'0-0'2'


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CONCLU/ION/

Co'o concl&siones $ene'os %&e el 7.6@ de lsec&enci de los genes HOXD5 de los dos o"gnis'osson si'il"es es$e !o"cen$e es '&) 2o ) %&e ldi#e"enci en el n4'e"o de !"es de 2ses es '&) l$en los 'is'os o"gnis'os $eniendo l Bos tars con

5?;? !2 ) l!enopscon 55 !2

Documents

Tutorial Genes Hox