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ICGEB Council of Scientific Advisers – Twenty-second session – Trieste, Italy, 17-18 May 2016
1
GENERAL PROGRAMME
Tuesday, 17 May 2016 09.00 - 10.30: CSA session: Plenary meeting
10.30 - 11.00: Coffee Break – ICGEB Atrium (CSA Members)
11.00 - 13.00: CSA session: Plenary meeting (cont.) 13.00 -14.00: Lunch Break – ICGEB Common Room (CSA Members)
14.00 -15.40: Scientific Presentations by ICGEB Research Groups (20+5’ per Group)
• Bacteriology • Biotechnology Development Unit
• Biosafety
• Human Molecular Genetics 15.40 - 16.00: Coffee Break – ICGEB Atrium
16.00 - 17.40: Scientific Presentations (continued)
• Cardiovascular Biology • Molecular Medicine
• Molecular Cardiology
• Molecular Hematology
17.45: Transportation to Hotels (Shuttle bus)
NEUROBIOLOGY GROUP
Fabian Feiguin [email protected]
ICGEBCluster Smart Health FVG
Trieste20 May 2016
Pathological profile: Extracellular and Intracellular Formation of Insoluble Protein Aggregates
How do these deposits form? How do they kill neurons?
Molecular Mechanisms of Neurodegeneration in Human Diseases
Drosophila melanogaster as a Model System to Understand Human Diseases
20000-25000 genes
From 714 human disease genes identified, 548 are conserved in Drosophila. 77% according to OMIM Database.
14000 genes
Thio
flavi
n-S
Act
in-P
hallo
idin
GM
R-g
al4,
UA
STa
u
GM
R-g
al4,
UA
STa
u/ d
RE
EP
w1118 +;Tau reepΔ275/Δ258;Tau UAS d-reep;Tau UAS h-reep;Tau
Mer
ge
The REEP1 Prevents the Formation of Neurotoxic Tau Aggregates
0
20
40
60
80
100
A C
020
40
60
80
100
Nor
m. a
rea
Glia
/Neu
ron
***
a-GFP a-HRP a-GFP a-HRPB
RTBc
RTBi RTBc
RTBi
T=29°C
LARVAL WAVES 29°C
Peris
talti
c w
aves
in 2
min
***
D
0
20
40
60
80
100
CLIMBING 29°C
% T
op s
corin
g fli
es
***
***
***
******
days4 7 14
E
4 7 140
25
50
75
100
0
25
50
75
100*** ***
0 10 20 30 40 50 60020406080100
F
Surv
ival
rate
(%)
LIFE SPAN ASSAY 29°C
days 0 10 20 30 40 50 60
0
20
40
60
80
100
***
RTBcRTBi
G
0
10
20
30
40 *
EJP
Ampl
itude
(mV)
RTBcRTBi
t(ms)
50 ms
10 m
V
EVOKED
EJP
Ampl
itude
(mV)
FM1-43
KCl
Rel
ativ
e la
bellin
g in
tens
ity (%
)0
20
40
60
80
100
unloadload
load
unload
RTB
c
RTB
i
0
20
40
60
80
100
Loss of TDP-43 in the Nervous System Affects Axonal Wrapping, Flies Locomotion and Life Span
Drug Screening Against High Glutamate-mediated Stress identifies New Pharmacological Compounds and Molecular Targets
0
25
50
75
100
0
10
20
30
40
50
Nor
m.
peris
talti
c w
aves
Nor
m.
peris
talti
c w
aves
Nor
m.
peris
talti
c w
aves
0
10
20
30
40
50
0
25
50
75
100
0
10
20
30
40
50
0
25
50
75
100
MELATONIN (2mM) ASCORBIC ACID (5.5mM) N-ACETYL CYSTEINE (3mM)
*
**
***
**
*
***
V V V VVV DDDDDD
TBPH-RNAi Glia
Control
V=vehicle D=drug0
25
50
75
100
0
25
50
75
100
Nor
m.
per
ista
ltic
wav
es
V V DD
***
ns
NDGA (1mM) LiCl (25mM) Riluzole (0.5mM)
VV DD0
25
50
75
100
VV DD0
25
50
75
100
VV DD
Nor
m.
peris
talti
c w
aves
Nor
m.
peris
talti
c w
aves
TBPH∆23/∆23
*
