Transcription in EukaryotesClass II promoters:

*Core Promoter

TATA box

TFIIB Recognition Element (BRE)

Initiator (Inr)

Downstream Promoter Element (DPE)

*Upstream Promoter Element

Transcription Initiation in Eukaryotes

General Transcription Factors and the Pre-initiation complex:

Transcription Initiation in EukaryotesTBP binding:

Transcription Initiation in Eukaryotes

Transcription Initiation in Eukaryotes

Schematic ModelElongation of Transcription in Eukaryotes

Polyadenylation

*CstF

*CPSF

*PAP

*PABP

RNA processing in eukaryotesAddition of poly A tail

Addition of 5’cap

Splicing

Torpedo model for transcription termination

Formation of the 5’ cap

*RNA triphosphatase

*guanylyl transferase

*methyl transferase

Transcript Processing

Functions of the Cap and Tail

ProtectionTranslatability TransportSplicing