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7/30/2019 Topic 3.4
http://slidepdf.com/reader/full/topic-34 1/3
Topic 3.4 Replication
Slide 1 – title page
Slide 2
- When does DNA replication take place?
- What are chromosomes made of?
- Before mitosis all DNA must be replicated – why?
- What are sister chromatids?
- What are the stages of the entire cell cycle?
- What does mitosis ensure?
- What is cyotkinesis?
Slide 3
- What does DNA helicase do?
- “unzipping” really means what?
Slide 4
- What does DNA polymerase do?
- DNA is a polymer – what does that mean?
- What is the difference between the parent strand and the complementary strand?
Slide 5
- What would a mistake in the order of bases result in?
- Click on the image and build a DNA molecule
Slide 6
- What is semi-conservative replication?
- Click on the “how do we know this?” pic and summarize the findings of the presented
experiment. (4-5 sentences)
Slide 7
- That was review – here begins the HL stuff
- Click on the links on these notes and on the slide – follow my (probably non-intuitiveinstructions)
- Under “DNA replication” with the purple oval on it - it will take you to a site of folders. Click on
this link for a direct transport to the overview:
http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/dna-rna2.swf
- Click on this link and then click on DNA replication
http://www.wiley.com//legacy/college/boyer/0470003790/animations/animations.htm
7/30/2019 Topic 3.4
http://slidepdf.com/reader/full/topic-34 2/3
- The link on slide 7 that says “DNA replication fork” doesn’t work
- The final link on slide 7 (the yellow one) works well and will take you to where you need to go.
Slide 8
- Why would replication take place in multiple locations in eukaryotes?
- What are the areas of replication known as in a eukaryote?
- How are these areas identified?
- What are the proteins called that act as a attachment site for DNA helicase?
- How is DNA replicated in prokaryotes?
- Click on the bidirectional DNA replication link (watch it)
Slide 9-12
- DNA replication moves in a __________________direction – this means the 5’ end of
_________________________
- What is a deoxyonucleoside triphosphate? (you can google it) Describe this molecule in thespace below.
- What type of a bond attaches the 5’ end to the 3’ carbon?
- Watch the video link on slide 12 - click on “how nucleotides are added in DNA replication”
Slide 13
- Replication on the lead strand is ___________
- What is the exposed prime of the parent strand of the leading strand?
Slide 14
- What enzyme is responsible for attaching new DNA nucleotides during replication?
- Replication on the lagging (lag) strand leap frogs – what does that mean?
- What is the exposed prime of the parent strand on the lagging strand?
- What direction does replication move in?
- What are used to mark positions on the lag strand?
Slide 15
- What are the steps of replication on the leading strand?
o o o
Slide 16
- What does RNA primase do?
7/30/2019 Topic 3.4
http://slidepdf.com/reader/full/topic-34 3/3
- What is the purpose of RNA primers in replication of the lag strand?
Slide 17
- What attaches to the RNA primer?
- What does DNA polymerase do once it reaches a new RNA primer?
- What are Okazaki fragments?
Slide 18
- What does DNA polymerase I do?
- What does DNA ligase do?
- Watch the DNA replication form video (upper right of slide)
- Click on the image on the bottom of the slide – click on and watch the whole picture tab. Watch
the whole picture twice – pay attention to the enzymes involved.
Slide 19-20
- Try to define all of these enzymes.
Slide 21 – good job!