Upload
duongcong
View
230
Download
0
Embed Size (px)
Citation preview
Page|25 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
T E C H N I C A L N O T E S
Titrationofcitricacidinjuice:TechnicalNotes
IntroductionThisisafairlystandardtitrationexceptforthefollowing:
• commerciallemonjuiceisused.Thisjuiceisslightlycoloured;and
• commerciallemonjuicehasatotalacidconcentrationthatisaround1M.Thisneedsdilutionbeforeuse.
Page|26 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Equipmentandmaterials• Safetyglassesandglovesfor
eachstudent
• Citricjuicesamples
• NaOHsolution(approx.0.1molL-1).
• Phenolphthaleinindicator
• Burette
• Retortstand
• Buretteclamp
• Pipette
• Pipettefiller(bulb)
• Titrationvessel(conicalflask)
• Beakers
• Funnel
• Whitetile
Riskmanagement• Thereisapossibilitythatincorrectlyusedglasswaremight
break,causingcutsorotherinjury.Withappropriateinstructionontheuseofburettes,pipettesandotherglassware,thereislowrisk.
• Fillingtheburettewhiletheburetteisplacedonabenchtopposestheriskofeyeinjurysincethesolutionsarebeingtransferredaboveeyelevel.Theburettestand(andtheburette)shouldbeplacedonthefloorwhilefilling,sothatthetopoftheburetteisbeloweye-level.
• Phenolphthaleinindicatorisflammable:keepawayfromignitionsources.Theamountsandconcentrationsofphenolphthaleinindicatorusedinthisexerciseposelowrisk.
Page|27 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
• Sodiumhydroxideiscorrosive.Theconcentrations(0.1M)ofsodiumhydroxideusedinthisexerciseposelowrisk.Studentsshouldweargloves,andimmediatelycleanupanyspills.
Studentsmustneverpipettebymouth:theymustalwaysusepipettefillers.
Studentsmustwearsafetyglasses/goggles.
MSDSSodiumhydroxidesolutionwouldnormallybemadeupbydissolvingsolidsodiumhydroxideindeionisedwatertogiveaverydilutesolution.ThisisatypicalMSDSforaconcentratedsodiumhydroxidesolution.<http://msds.orica.com/pdf/shess-en-cds-010-000031006701.pdf>.
Page|28 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|29 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|30 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|31 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|32 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|33 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|34 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|35 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
PhenolphthaleinIndicatorsolutionisavailableindifferentconcentrationsandindifferentsolvents.ThisisatypicalMSDS.<http://file.southernbiological.com/Assets/Products/Chemicals/Stains_and_Indicators-Powders/SIP13_5-Phenolphthalein/SIP13_5_MSDS_2011_Phenolphthalein.pdf>.
Page|36 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|37 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|38 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|39 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Page|40 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
SuppliersLemonjuicecanbesourcesfromanysupermarket.Forexample,thefollowingisavailablefromWoolworths:
• WoolworthsSelectLemonJuice500mL
Page|41 TechnicalNotes-Titrationofcitricacidinjuice–StIgnatiusCollegeGeelong
Sodiumhydroxide(draincleaner)canalsobesourcedfromasupermarket.Itwillbeoflowerpuritythansodiumhydroxidefromaspecialistchemicalsupplier.Sincesodiumhydroxideishydroscopic,anysodiumhydroxideshouldbestandardisedbeforeuse.
PreparationsuggestionsTheamountoftotalacidinlemonjuiceisapproximately1molL-1.Thelemonjuiceshouldbedilutedbymixing1volumeoflemonjuicewith9volumesofdeionisedwater.500mLlemonjuicewillmakeup5Lofdilutedlemonjuice.
StoragesuggestionsSodiumhydroxidesolutionshouldbefreshlymadewithinafewdaysasthesolutionwillreactwithcarbondioxideintheatmosphere.Anysodiumhydroxidesolutionthathasbeenstoredformoreafewdaysshouldbere-standardisedbeforeuse.
CopyrightandCreativeCommonsThemoralrightsoftheauthors,KieranLim,EliseMeehan,JessicaSaw,PetaWhite,JohnLong,andIanBentley,havebeenassertedundertheAustralianCopyrightAct1968(Cth).Exceptinglogos,trademarksorotherthird-partycontentasindicated,thisresourceisdistributedunderaCreativeCommons‘Attribution-NonCommercial-ShareAlike’4.0InternationalLicense.