The protein kinase Pstol1 from traditional riceconfers tolerance of phosphorus deficiency

12-04-2015

Gamuyao et al., 2012. Nature

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Biological role

Phosphorus (P) is an essential macronutrient for plant growth

It is central to a majority of the molecular constituents required for the functioning of plant cells.

DNA; RNA; Proteins; Lipids; Sugars, ATP, ADP, and NADPH

It is indispensable in agricultural production

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The problem of phosphorus

Kochian Nature 488, 466–467 (2012) doi:10.1038/488466a

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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

Origin of the donor variety Kasalath

• aus-type variety

Aoosh (early)

This group of rice is famously known to tolerate difficult growing conditions

• Pup1

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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

Phosphorus uptake 1 (Pup1) candidate genes

OsPupK46-2

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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

Postl 1 is the major genetic determinant of P-deficiency tolerance

Phosphorus-Starvation Tolerance 1

OsPupK46-2

Nipponbare near-isogenic lines(NILs) -P conditions With or without P fertilizer

Which one is the major determinant of P-deficiencytolerance in Kasalath?

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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

Pstol1 is a functional protein kinase

in vitro phosphorylation assay

How Pstol1 involved with the P-efficient ?

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PSTOL1 overexpression enhances tolerance of P deficiency

R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

35S::PSTOL1

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Indica

Japonica

The larger root system also enhanced the uptake of other nutrients

R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

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How Pstol1 involved with the P-efficient?

By enhancing root growth?

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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

PSTOL1 is an enhancer of root growth and independent of phosphorus or soil-related conditions

100mM

10mM

Really ?

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Pstol1

IR64 transgenics

R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

RNA interference in Kasalath

PSTOL1 is an enhancer of root growth!

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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

How PSTOL1 enhance root growth?

The expression of PSTOL1 during root development

PSTOL1 is a regulator of early crown root development and root growth

Crown root Main root system

Crown Root Primordia

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Parenchymatic cells

vascular bundles

Emerging crown roots

R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

PSTOL1 putative downstream genes

23 genes related to root growth and stress response with constitutively higher or lower expression in the transgenic plants were identified

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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346

PSTOL1 putative downstream genes

21 of these differentially expressed genes co-localize with QTLs related to drought tolerance and root growth

a positive regulator ofroot cell differentiation

delay leaf senescencein rice

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