The Microscope. Definition A compound microscope is an optical instrument used to magnify and...
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The Microscope. Definition A compound microscope is an optical instrument used to magnify and resolve fine detail within a transparent specimen. It has
Definition A compound microscope is an optical instrument used
to magnify and resolve fine detail within a transparent specimen.
It has two separate lenses: (1) The objective lens and (2) the
eyepiece
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Function of Microscope Parts
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Eyepiece The topmost optical lens system, also called the
ocular. This is where the eye is positioned to view the object.
Magnifies 10X Eyepiece
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Pointer Used to locate an object being viewed under the scope.
Turning the eyepiece changes the pointer. Pointer (inside)
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Body Tube This is where the rays of light are bent so that the
object being viewed is enlarged by the scope Body Tube
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Arm Supports the body tube and the objectives. Used to carry
the microscope Arm
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Low Power Objective Magnifies 10x and is found above the
specimen. Always begin focusing under low power Low Power (smallest
objective)
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Medium Power Magnifies 25x. Use second after low power. Medium
Power (middle sized objective)
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High Power Objective Magnifies 65x and is used last for
greatest detail High Power (largest objective)
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Coarse Adjustment Knob The larger set of knobs used for coarse
focusing under low power Coarse Adjustment Knob
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Fine Adjustment Knob Smaller set of focusing knobs used to
control fine focusing under medium and high power Fine Adjustment
Knob
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Base Supports the scope. Also used to carry the scope along
with the arm Base
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Stage Flat area that supports the microscope slide Stage
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Stage Clips Hold the slide in place Stage Clips
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In-Stage Condenser Focuses and concentrates the light coming
through the stage so that the object is correctly illuminated Aka
aperture In-Stage Condenser
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Diaphragm The rotatable disc located beneath the stage that
controls the amount of light entering the in- stage condenser
Diaphragm
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Nosepiece Supports the revolving nosepiece Nosepiece
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Revolving Nosepiece Supports the objectives and is used to
change from low to high Revolving Nosepiece
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Resolution Vs. Magnification
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Resolution The ability of a microscope to distinguish two
objects as being separate
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Magnification The ability of a microscope to make objects
appear larger.
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Resolution is more important than magnification!
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Magnification
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The magnification of the low power objective is 10x
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The magnification of the medium power objective is 25x
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The magnification of the high power objective is 65x
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The magnification of the eyepiece is 10x Eyepiece
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To find the Total Magnification you must Multiply Low Power X
Eyepiece = Total magnification Under low power 10 x 10 = 100
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Carrying a Microscope
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1. One hand is placed around the arm and the other is placed
under the base.
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2. Always hold in an upright position
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3. The stage clips should be turned inward
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4. Eyepeice at home position on low power.
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5. Body tube is in home position (all the way up)
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6. All slides have been put away and are no longer on the
stage.
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7. Cord is unplugged and wrapped around the base
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Procedures for Focusing the Microscope
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1. Put the microscope on low power and turn the stage all the
way down
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2. Place the microscope slide on the stage and secure with
stage clips
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3. While looking through the eyepiece, turn the coarse adjuster
toward you until the object comes into focus
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4. Turn the objective to medium power and refocus while looking
through the eyepiece using the fine adjuster
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5. Turn the objective to high power and refocus while looking
through the eyepiece using the fine adjuster
http://www.youtube.com/watch?v=zDw_R3Y76yM&feature=related
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eyepiece pointer Arm Stage Clip Coarse Adjustment Fine
Adjustment Power SwitchBase Light Diaphragm Stage (opening)
In-Stage Condenser Objectives Revolving Nosepiece Nosepiece (whole
thing) Body tube Stage Stop
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Lab Safety: What NOT to do!
http://www.youtube.com/watch?v=vRIA04U796E&feature=related