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The iGEM Jamboree at MIT

The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

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Page 1: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

The iGEM Jamboree at MIT

Page 2: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

iGEM 20045 teams

Page 3: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

iGEM 200513 teams

Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley Davidson Harvard UCSF

Page 4: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley Davidson Harvard UCSF

iGEM 200513 teams

Page 5: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

iGEM 200632 teams

Page 6: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Putting the “i” in iGEM

Page 7: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

200754 teams

Page 8: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

200884 teams

Page 9: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

2009112 teams

Page 10: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 11: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Chemistry

Mathematics Computer Science

Page 12: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

How is synthetic biology well-suited for interdisciplinary research?

Environment Energy Medicine Technology

Creativity Flexibility

If you have a common interest, you can find a synthetic biology way to approach it!

Adaptability

Page 13: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Synthetic Biology Project Exampleshttp://2010.igem.org/Main_Page

Page 14: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

highGEM

Greenfield Central High School, Greenfield, INRebecca Schinii [email protected]

Page 15: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Ethics/ Human Practice

Page 16: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Construct Version

Synthetic biology uses molecular methods to construct DNA-based devices to perform novel functions. Synthetic biologists design, model, and construct biological parts, devices and systems by engineering and modifying existing biological systems. For instance, synthetic biologists can produce microbes that make useful chemicals. Synthetic biology gives scientists the tools to build biological devices from smaller biological units in much the same way as an engineer constructs a building from smaller construction materials. Synthetic biology has the potential to develop technologies that could lead the way in the 21st century.

Page 17: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Create Version

Synthetic biology uses molecular methods to create modified living organisms that have never existed before. These DNA-based devices perform new functions not found in nature. Synthetic biologists redesign and modify existing, natural biological systems. For instance, synthetic biologists can create new versions of microbes that make chemicals not naturally found in microbes. In many ways, synthetic biology allows scientists to redesign biological processes and create organisms not found in nature. Synthetic biology has the potential to develop technologies that could lead the way in the 21st century.

Page 18: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Ethics and Word Choice

Page 19: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Ethics and Word Choice

Page 20: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Energy

Page 21: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

2008 Project by Harvard iGEM Team

http://2008.igem.org/Team:Harvard

Page 22: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Shewanella oneidensis MR-1

Page 23: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 24: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 25: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 26: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 27: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Four 2009 iGEM Projects

Page 28: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

http://2009.igem.org/Team:HKU-HKBU

Page 29: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

http://2009.igem.org/Team:HKU-HKBU/Assembly#Step_6._Using_the_Speed_Controller

Page 30: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

http://2009.igem.org/Team:HKU-HKBU/Assembly#Step_6._Using_the_Speed_Controller

Page 31: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Environment

Page 32: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Edinburgh iGEM 2006

Awards: Best Real World Application, Best Poster

Page 33: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Poison Water

• Arsenic poisoning affects 100 million people

• Serious in Bangladesh

• Wells tap sedimentary layer with Arsenic• 1 in 4 wells contaminated

• Testing methods expensive, require instruments and expertise

Page 34: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Range of Outputs

If no arsenic is present: Urease raises the pH to 9-10

If 5 ppb arsenic is present: Repressor shuts down Urease, pH remains neutral, pH 7

If 20 ppb arsenic is present: LacZ produced, pH 4.5

Page 35: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 36: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Technology

Page 37: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

http://parts.mit.edu/igem07/index.php/Peking

Page 38: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Toggle Switch

Page 39: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Toggle Switch

A B Output

0 0 1

0 1 0

1 0 0

1 1 0

NOR Logic Gate

Page 40: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Toggle Switch

Page 41: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Explore the 2010 iGEM Wiki

Google search iGEM 2010 (first hit)

http://2010.igem.org/Main_Page

Page 42: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 43: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 44: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 45: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 46: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley
Page 47: The iGEM Jamboree at MIT. iGEM 2004 5 teams iGEM 2005 13 teams Princeton Oklahoma ETH Zurich MIT Caltech Toronto Cambridge Texas Penn State Berkeley

Go explore!!