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1. The morphology and biology of the
fungi
Classification of medically
important fungi
• Fungal morphology and structure
• Antifungal (AF) - Mechanisms of action
• Fungi - biology and physiology
• Fungal infections (FI) - classification
The goal of teaching: 2. Common fungi which primarily cause skin,
hair and nail FI
• Malassezia - morphology, biology
Pathogenesis of pitiriasis versicolor,
Pathogenesis of seborrheic dermatitis
• Trichophyton - morphology, biology
• Microsporum - morphology, biology
• Epidermophyton - morphology, biology
Pathogenesis of dermatomycoses
3. Common fungi which causes
superficial / invasive / oprtunistic FI
• Candida - morphology, biology
Pathogenesis
• Cryptococcus - morphology, biology.
Pathogenesis
• Aspergillus - morphology, biology.
Pathogenesis
• Fusarium - morphology, biology.
Pathogenesis
4. Other fungi which causes of superficial /
invasive oprtunistic FI
• Penicillium - morphology, biology
Pathogenesis
• Zygomycetes (Mucor, Rhisopus, Absidia) -
morphology, biology.
Pathogenesis
• Pneumocystis - morphology, biology.
Pathogenesis
COMMON CAUSES OF FI Skin, hair, nail:
Malassezia
Trichophyton
Microsporum
Epidermophyton
Candida
Superficial mycoses: :
Malassezia
Exophiala (Cladosporum) werneckii
Trichosporon beigelli
Piedraea hortae
...
Cutaneous mycoses
dermatomycoses / tinea: Trichophyton
Microsporum
Epidermophyton
Candida
Aspergillus
…
Mucocutaneous mycoses:
Candida
Geotrichum
Rhinosporidium seeberi
...
Othomycoses:
Aspergillus
Candida
...
Keratomycoses:
Fusarium
Aspergillus
Onichomycoses:
Candida
Dermatophytes
Undermatophytes moluds
Malassezia (Pityrosporum)
• Yeasts, NF of skin, lipophilic species:
M.furfur, M.sympodialis, M.globosa...
• Unlipophilic species:
M. pahidermatis
Pathogenesis: • Pityriasis versicolor
(tinea versicolor)
• Malassezia foliculitis
• Seborrheic dermatitis (pityriasis
simplex capitis - dandruff)
• Atopic dermatitis
(systemic infection - rare / lipid th)
LDg/ DMP
Culture
Th/ AF local
MM2
Pytiriasis versicolor (tinea versicolor)
• Slight, recurrent disease of the
superficial layers of the skin (stratum corneum)
• Lesions- with small circular
flakes (scaling)
• Predisposing factors:
sweating, gender, genetic basis ...
• Predilection sites:
chest, back, shoulders
• System types: infants and adults, CVC
DDG: erithrasma, vitiligo
Hypo-pigmentation Hyper-pigmentation
Dermatophytes
Trichophyton (around 20 species)
Microsporum (around 17 species)
Epidermophyton (2 species)
• Highly contagious, cosmopolitan distribution
• Keratinophilic: hair, nails, and superficial layer of skin
(reproduction)
Anthropophilic species
Zoophilic species
Geophilic species
Dermatophytes
Zoophilic species:
Microsporum
M. canis
M. equinum
M. gallinae
Trichophyton
T. equinum
T. mentagrophytes
T. simii
T. verrucosum
The most frequent species in human: • Trichophyton rubrum
• Trichophyton interdigitale
• Epidermophyton floccosum
• Microsporum canis
• Trichophyton verrucosum
Geophilic species:
Microcporum
M. gypseum
M.nanum
M.persicolor
Trichophyton
T. ajeloii
Antropophilic species:
Microsporum
M. audouinii
M. ferrugineum
Trichophyton
T. concentricum
T. interdigitale
T. rubrum
T. schoenleinii
T. tonsurans
T. violaceum
Epidermophyton
E. floccosum
Source of infection: people, animals, soil
Dermatophytes
Trichophyton
Microsporum
Epidermophyton
Macroconidia
(rare)
Microconidia
(numerous)
Macroconidia
(numerous)
Microconidia
(rare)
Macroconidia
(only)
Microconidia
(absent)
• Macroconidia / multicellular
fusiform
cylindrical
drumstick shape
• Microconidia / unicellular
pyriform
in clusters
Microscopic characteristics of
dermatophytoses
D E R M A T O F I T I
Localization of dermatophytes and
characteristics essential for identification
Trichophyton I group
(skin, nails, hair - type ectotrix)
» T. mentagrophytes
» T. rubrum
» T. verrucosum
Trichophyton II group
(skin, nails, hair - type endotrix)
» T. tonsurans
» T. schoenleinii
» T. violaceum
macroconidia rare (pencil shaped 3-8 cells ) numerous microconidia in clusters
Microsporum
(hair and skin)
Numerous macroconidia
(elliptical, fusiform 3-7 cells)
Macrodonidia by hyphae, one
Epidermophyton
(skin and nails)
Only macroconidia
(rounded, with 2-4 cells)
DERMATOPHYTOSES TINEA/RINGWORM/DERMATOMYCOSES
Indicate infection of the superficial layers of skin, hair and nails
caused by dermatophytes or other fungi
DERMATOPHYTOSIS (Tinea)
localization
Trichophyton
(around 20 species)
Skin
Hair
Nails
Herpes tonsurans
LDg/ DMP
Culture
Th/ AF MM2
Herpes tonsurans
LDg/ DMP
Culture
Th/ AF
MM2
Herpes tonsurans Trichophytia superficialis
Trichophyton mentagrophytes
LDg/ DMP
Culture
Th/ AF MM2
Kerion Celsii Sycosis barbae
Trichophytia profunda
LDg/ DMP
Culture
Th/ AF MM2
Kerion Celsi
LDg/ DMP
Culture
Th/ AF MM2
Sycosis barbae
LDg/ DMP
Culture
Th/ AF MM2
Favus -Trichophyton schoenleinii
LDg/ DMP
Culture
Th/ AF MM2
Tinea imbricata - T. concentricum
Trichophyton mentagrophytes v. interdigitale
Onychomycosis - T. mentagrophytes
Itraconazole pulse
Dr Decroix , Belgium
Microsporum
(17 species)
Skin
Hair – type ectotrix
rare - nails
Microsporum gypseum
Microsporum canis
Microsporum gypseum Microsporum canis
Microsporia
LDg/ DMP
Culture
Th/ AF
Epidermophyton
(2 species)
Skin
Nails
Epidermophyton floccosum
LDg/ DMP
Culture
Th/ AF
Tinea corporis
Tinea pedis
Epidermophyton floccosum
Macroconida (phase contrast 400X) Macroconidia (LFCB 400X)
Dermatophytid – immunopatological manifestation
Fungal Ag in complex with Ab in the skin
Sima Milošević (1896-1943)
Epidermophyton floccosum (Harz) Langeron et Milochevitch
Trichophyton schoenleinii (Lebert) Langeron et Milochevitch
Langeron, 1950.
Trichophyton milochevitch