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cgtggaaaaataagt----ttgtccactggaacgtctcaaaacagttccaactg
xxxx xx xxxx xxxxxxxxxxxxxxxxxxxxxxxx
------ggctcaagtaacctttcccacaccaacgtctcaaaacagttccaactg
ctgagaaaacacac-cg-cttgaaggccaagaagagaggtatttatag-cgtgg
x xxxxxxxxxxxx xx xxxxx x xxxxx x xxxxxxxxxxx xxxxx
ctgagaaaacacacacgacttgaggttg-agaagcggggtatttataggcgtgg
gccttggatgagctatgagcacgcgaaagatgattggacaataaatgttccttg
x xxxxxxxxxxxxxx x xxx x xx xxxxxxxxxx xxx xx x xx
acattggatgacgtatgggtacatgcaatctgattggacagtaagtgctgggtg
tatgcaaataaggggaattcaagtggtgctttctgattggttaattcatcacca
xxxxxxxxxxxxxxxxxxxxxx xxx xxxxx xxxxxxxxxx xxxxx xx
catgcaaataaggggaattcaagctgtgatttctaattggttaatccatcatca
atcagccaatcaggtggtgagtgtaatttgccattagactataaataggcgtgg
xxxxxxxxxxxxxxxx xxxx xxx xxxxxxxxxxxxxxxxxxxxxxxxxx
atcagccaatcaggtgttgagcataagctgccattagactataaataggcgtgg
acgcacta-cctccgct-acggctttatggcttttgaagaatc
x xxxx xxx xx xxxxxx x xxxxxxxxxxxxx
tcatgggggcctc-gcttaccgctttaagacttttgaagaatc
Th2a
Th2b
Ra
tM
ou
se
TATA
Hexamer CCAAT
Octamer CCAAT
CCAAT TATAOctamer
Approximate TSS positions
BamHINeo
GAPHHHHHHGAAGGDYKDDDDKSAAGGYPYDVPDYA
8.8 kb
BamHI
LoxP
TAG
Th2b
LoxP
His Flag Ha
Th2a //
BamHI
8.8 kb
BamHITAG
X CMV - Cre
Th2bTh2aTh2a //
Th2bNeo/Neo
Th2b +/tag
A
B
Montellier et al. Figure S1
TH2B-less
TH2B-tag
PCR
Th2bNeo
Wild type
1 2 3Southern
12.7
8.8
BamHI digest
C
Th2bTh2a
BamHI
BamHI
BamHI BamHI
12.7 kb// Th2b+/+
TH2B
3’ probe
3’ probe
3’ probe
3’ probe
1 2 3
PCR primers
Mass spectrometry analysis of histone variants
Montellier et al. Figure S2 (related to Figure 4A and B)
Montellier et al. Figure S3
+ -H2AZ + -
Spc R-Spt
Expression profile of H2AZ+
versus H2AZ- genes
Nor
mal
ized
expr
essi
on v
alue
s
p<0.0001* p=0.0004*
B TH2BGFP
tagTH2BGFP
A (related to Figure 4 D to F)
Montellier et al. Figure S4 (related to Figure 5B)
Th2
b+/
+Th
2b
+/t
ag
TH2B TP2 TH2B Prm1
Co-detection of TH2B and TP2 Co-detection of TH2B and Prm1
11500 12000 12500 13000 13500 14000 14500
Mass (Da)
0
10
20
30
40
50
60
70
80
90
100
0
10
20
30
40
50
60
70
80
90
100
Rela
tive
Ab
un
da
nc
e
11331.39
14208.75
13860.44
Th2b +/+
TH2B -less
H4
+1Ac
13916.50
tH2B
H2B type 1-H
and/or 1-K and/or 2-B
13700 13800 13900 14000 14100 14200 14300 14400 14500
Mass (Da)
0
10
20
30
40
50
60
70
80
90
100
0
10
20
30
40
50
60
70
80
90
100
13860.44
H2B type 1-F/J/L
and/or 1-M
TH2B
14208.75
H2A
Type 2-A
13916.50
H2B type 1-P (x7.3)
13876.60
H2B type 1-B (x4.0)
H2B
type 1-C/E/G(x2.4)
H2B
type 1-H
and/or 1-K
and/or 2-B(x2.6)
H2B type 1-F/J/L or 1-M (x2.8)
H2A type 2-A (x1.1)
Montellier et al. Figure S5A (related to Figure 6B)
-0.20
0.00
0.20
0.40
0.60
0.80
1.00
1.20
1.40
1.60
tH2B H2A type 2-A H2B type 1-P Histone H2B type 1-B Histone H2B type 1-F/J/L and/or 1-M
Histone H2B type 1-H or 1-K and/or 2-B
Histone H2B type 1-C/E/G
H2
B/H
4 r
atio
tH2B +/+
tH2B -/-
x1.1
x7.3 x4.0
x2.8
x2.6 x2.4
Th2b +/+
TH2B - less
Montellier et al. Figure S5B (related to Figure 6B)
1min 3min 5min MNase digestion
Th2
b+/
+
Nucleosomes
Sub-nucleosomal particles
TH2
B-l
ess
Th2
b+/
+
TH2
B-l
ess
Th2
b+/
+
TH2
B-l
ess
Figure S5C(related to Figure 6)
MS/MS spectra of the modified peptides in histones
Montellier et al. Figure S5D (related to Figure 6C)
Monoisotopic mass of neutral peptide Mr(calc): 1496.84 Variable modifications: N-term : Propionyl:13C(3) (N-term) K2 : Acetyl (K) K5 : Acetyl (K) K9 : Acetyl (K) K13 : Acetyl (K) Ions Score: 91 Expect: 4.8e-008
MS/MS Fragmentation of GKGGKGLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 17530: 1496.836954 from(749.425753,2+)
Pr-GKacGGKacGLGKacGGAKacR H4K5ac, K8ac, K12ac; K16ac
Monoisotopic mass of neutral peptide Mr(calc): 982.56 Variable modifications: N-term : Propionyl (N-term) K4 : Acetyl (K) K8 : Acetyl (K) Ions Score: 40 Expect: 0.0023
MS/MS Fragmentation of GLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 6489: 982.553324 from(492.283938,2+)
Pr-GLGKacGGAKacR H4K12ac, K16ac
Monoisotopic mass of neutral peptide Mr(calc): 1513.87 Variable modifications: N-term : Propionyl:13C(3) (N-term) K2 : Propionyl:13C(3) (K) K5 : Acetyl (K) K9 : Acetyl (K) K13 : Acetyl (K) Ions Score: 70 Expect: 6.6e-006
MS/MS Fragmentation of GKGGKGLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 27868: 1513.863074 from(757.938813,2+)
Pr-GKprGGKacGLGKacGGAKacR H4K8ac, K12ac, K16ac
Monoisotopic mass of neutral peptide Mr(calc): 1450.81 Variable modifications: N-term : Propionyl (N-term) K8 : Propionyl (K) R12 : Methyl (R) Ions Score: 71 Expect: 2.9e-006
MS/MS Fragmentation of DNIQGITKPAIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 23701: 1450.809604 from(726.412078,2+)
Pr-DNIQGITKprPAIRme H4R35me
MS/MS Fragmentation of ISGLIYEETRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 10921: 1249.653234 from(625.833893,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1249.66 Variable modifications: N-term : Propionyl (N-term) R10 : Methyl (R) Ions Score: 57 Expect: 4.8e-005
Pr-ISGLIYEETRme H4R55me
MS/MS Fragmentation of GVLKVFLENVIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 15906: 1512.899574 from(757.457063,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1512.90 Variable modifications: N-term : Propionyl:13C(3) (N-term) K4 : Crotonoyl (K) Ions Score: 73 Expect: 9.3e-007
Pr-GVLKCrVFLENVIR H4K59cr
Monoisotopic mass of neutral peptide Mr(calc): 1058.61 Variable modifications: N-term : Propionyl (N-term) R8 : Methyl (R) Ions Score: 49 Expect: 0.00025
MS/MS Fragmentation of VFLENVIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 13932: 1058.610514 from(530.312533,2+)
Pr-VFLENVIRme H4R67me
Monoisotopic mass of neutral peptide Mr(calc): 1413.69 Variable modifications: N-term : Propionyl (N-term) K10 : Crotonoyl (K) Ions Score: 41 Expect: 0.002
MS/MS Fragmentation of DAVTYTEHAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 26245: 1413.683874 from(707.849213,2+)
Pr-DAVTYTEHAKcrR H4K77cr
Monoisotopic mass of neutral peptide Mr(calc): 1376.70 Variable modifications: N-term : Propionyl:13C(3) (N-term) K10 : Dimethyl (K) Ions Score: 57 Expect: 5.9e-005
MS/MS Fragmentation of DAVTYTEHAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 13738: 1376.664954 from(689.339753,2+)
Pr-DAVTYTEHAKme2R H4K77me2
Monoisotopic mass of neutral peptide Mr(calc): 1753.97 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K13 : Acetyl (K) Ions Score: 45 Expect: 0.00098
MS/MS Fragmentation of KTVTAMDVVYALKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 23128: 1754.007234 from(878.010893,2+)
Pr-KprTVTAMDVVYALKacR H4K79ac
Monoisotopic mass of neutral peptide Mr(calc): 1060.60 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K6 : Acetyl (K) Ions Score: 53 Expect: 0.00013
MS/MS Fragmentation of KSTGGKAPRFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 8527: 1060.595124 from(531.304838,2+)
Pr-KprSTGGKacAPR H3K14ac
Monoisotopic mass of neutral peptide Mr(calc): 1125.65 Variable modifications: N-term : Propionyl (N-term) K1 : Acetyl (K) K6 : Acetyl (K) Ions Score: 46 Expect: 0.00089
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 14486: 1125.647494 from(563.831023,2+)
Pr-KacQLATKacAAR H3K18ac, K23ac
Pr-KprQLATKacAAR H3K23ac
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 10716: 1145.683264 from(573.848908,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1145.69 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K6 : Acetyl (K) Ions Score: 74 Expect: 5.5e-007
Monoisotopic mass of neutral peptide Mr(calc): 1165.68 Variable modifications: N-term : Propionyl (N-term) K1 : Propionyl (K) K6 : Crotonoyl (K)Ions Score: 48
Expect: 0.00041
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 20231: 1165.678994 from(583.846773,2+)
Pr-KprQLATKcrAAR H3K23cr
Monoisotopic mass of neutral peptide Mr(calc): 1070.60 Variable modifications: N-term : Propionyl (N-term) K1 : Dimethyl (K) K10 : Propionyl (K) Ions Score: 46 Expect: 0.0005
MS/MS Fragmentation of KSAPSTGGVKFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 12259: 1070.593544 from(536.304048,2+)
Pr-Kme2SAPSTGGVKpr H3K27me2
Monoisotopic mass of neutral peptide Mr(calc): 1090.60 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Acetyl (K) K10 : Propionyl:13C(3) (K)Ions Score: 46 Expect: 0.00025
MS/MS Fragmentation of KSAPSTGGVKFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 13085: 1090.628694 from(546.321623,2+)
Pr-KacSAPSTGGVKpr H3K27ac
Monoisotopic mass of neutral peptide Mr(calc): 1507.84 Variable modifications: N-term : Propionyl (N-term) K6 : Crotonoyl (K) Ions Score: 49 Expect: 0.00035
MS/MS Fragmentation of VTIMPKDIQLARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 24307: 1507.840244 from(754.927398,2+)
Pr-VTIMPKcrDIQLAR H3K122cr
Monoisotopic mass of neutral peptide Mr(calc): 1661.90 Variable modifications: N-term : Propionyl (N-term) K3 : Acetyl (K) K14 : Propionyl (K) Ions Score: 59 Expect: 3.9e-005
MS/MS Fragmentation of VLKQVHPDTGISSKFound in gi|30061387, histone H2B type 1-H [Mus musculus]
Match to Query 29675: 1661.934114 from(831.974333,2+)
Pr-R-VLKacQVHPDTGISSKpr H2BK46ac
Monoisotopic mass of neutral peptide Mr(calc): 1812.85 Variable modifications: N-term : Propionyl (N-term) R15 : Methyl (R)Ions Score: 72
Expect: 1.7e-006
MS/MS Fragmentation of AMGIMNSFVNDIFERFound in gi|68226433, histone H2B type 2-B [Mus musculus]
Match to Query 19777: 1812.850134 from(907.432343,2+)
Pr-AMGIMNSFVNDIFERme H2BR72me
Monoisotopic mass of neutral peptide Mr(calc): 1916.06 Variable modifications: N-term : Propionyl (N-term) K9 : Acetyl (K) K17 : Propionyl (K) Ions Score: 58 Expect: 9.3e-005
MS/MS Fragmentation of LLLPGELAKHAVSEGTKFound in gi|68226433, histone H2B type 2-B [Mus musculus]
Match to Query 21276: 1916.095494 from(959.055023,2+)
Pr-LLLPGELAKacHAVSEGTKpr H2BK108ac
Monoisotopic mass of neutral peptide Mr(calc): 1889.06 Variable modifications: N-term : Propionyl:13C(3) (N-term) K9 : Crotonoyl (K) Ions Score: 73 Expect: 2.5e-006
MS/MS Fragmentation of LLLPGELAKHAVSEGTKFound in gi|30089704, histone H2B type 1-K [Mus musculus]
Match to Query 22135: 1889.056794 from(945.535673,2+)
Pr-LLLPGELAKcrHAVSEGTK H2B108cr
Monoisotopic mass of neutral peptide Mr(calc): 1406.74 Variable modifications: N-term : Propionyl (N-term) K8 : Crotonoyl (K) K12 : Propionyl (K) Ions Score: 46 Expect: 0.00092
MS/MS Fragmentation of HAVSEGTKAVTKFound in gi|30061387, histone H2B type 1-H [Mus musculus]
Match to Query 26107: 1406.736974 from(704.375763,2+)
Pr-HAVSEGTKcrAVTKpr H2BK116cr
Monoisotopic mass of neutral peptide Mr(calc): 1386.74 Variable modifications: N-term : Propionyl:13C(3) (N-term) K8 : Acetyl (K) K12 : Propionyl:13C(3) (K) Ions Score: 79 Expect: 3e-007
MS/MS Fragmentation of HAVSEGTKAVTKFound in gi|13386452, histone H2B type 3-A [Mus musculus]
Match to Query 22240: 1386.776764 from(694.395658,2+)
Pr-HAVSEGTKacAVTKpr H2BK116ac
Monoisotopic mass of neutral peptide Mr(calc): 919.56 Variable modifications: N-term : Propionyl (N-term) R7 : Methyl (R) Ions Score: 38 Expect: 0.0008
MS/MS Fragmentation of HLQLAIRFound in gi|817939, histone H2A [Mus musculus domesticus]
Match to Query 5038: 919.556554 from(460.785553,2+)
Pr-HLQLAIRme H2AR96me
Monoisotopic mass of neutral peptide Mr(calc): 1300.68 Variable modifications: N-term : Propionyl (N-term) K1 : Propionyl (K) K7 : Crotonoyl (K) K9 : Propionyl (K) Ions Score: 60 Expect: 1.4e-005
MS/MS Fragmentation of KTESHHKAKFound in gi|817939, histone H2A [Mus musculus domesticus]
Match to Query 23609: 1300.675324 from(651.344938,2+)
Pr-KprTESHHKcrAKpr H2AK133cr
Monoisotopic mass of neutral peptide Mr(calc): 1496.84 Variable modifications: N-term : Propionyl:13C(3) (N-term) K2 : Acetyl (K) K5 : Acetyl (K) K9 : Acetyl (K) K13 : Acetyl (K) Ions Score: 91 Expect: 4.8e-008
MS/MS Fragmentation of GKGGKGLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 17530: 1496.836954 from(749.425753,2+)
Pr-GKacGGKacGLGKacGGAKacR H4K5ac, K8ac, K12ac; K16ac
Monoisotopic mass of neutral peptide Mr(calc): 982.56 Variable modifications: N-term : Propionyl (N-term) K4 : Acetyl (K) K8 : Acetyl (K) Ions Score: 40 Expect: 0.0023
MS/MS Fragmentation of GLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 6489: 982.553324 from(492.283938,2+)
Pr-GLGKacGGAKacR H4K12ac, K16ac
Monoisotopic mass of neutral peptide Mr(calc): 1513.87 Variable modifications: N-term : Propionyl:13C(3) (N-term) K2 : Propionyl:13C(3) (K) K5 : Acetyl (K) K9 : Acetyl (K) K13 : Acetyl (K) Ions Score: 70 Expect: 6.6e-006
MS/MS Fragmentation of GKGGKGLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 27868: 1513.863074 from(757.938813,2+)
Pr-GKprGGKacGLGKacGGAKacR H4K8ac, K12ac, K16ac
Monoisotopic mass of neutral peptide Mr(calc): 1450.81 Variable modifications: N-term : Propionyl (N-term) K8 : Propionyl (K) R12 : Methyl (R) Ions Score: 71 Expect: 2.9e-006
MS/MS Fragmentation of DNIQGITKPAIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 23701: 1450.809604 from(726.412078,2+)
Pr-DNIQGITKprPAIRme H4R35me
MS/MS Fragmentation of ISGLIYEETRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 10921: 1249.653234 from(625.833893,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1249.66 Variable modifications: N-term : Propionyl (N-term) R10 : Methyl (R) Ions Score: 57 Expect: 4.8e-005
Pr-ISGLIYEETRme H4R55me
MS/MS Fragmentation of GVLKVFLENVIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 15906: 1512.899574 from(757.457063,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1512.90 Variable modifications: N-term : Propionyl:13C(3) (N-term) K4 : Crotonoyl (K) Ions Score: 73 Expect: 9.3e-007
Pr-GVLKCrVFLENVIR H4K59cr
Monoisotopic mass of neutral peptide Mr(calc): 1058.61 Variable modifications: N-term : Propionyl (N-term) R8 : Methyl (R) Ions Score: 49 Expect: 0.00025
MS/MS Fragmentation of VFLENVIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 13932: 1058.610514 from(530.312533,2+)
Pr-VFLENVIRme H4R67me
Monoisotopic mass of neutral peptide Mr(calc): 1413.69 Variable modifications: N-term : Propionyl (N-term) K10 : Crotonoyl (K) Ions Score: 41 Expect: 0.002
MS/MS Fragmentation of DAVTYTEHAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 26245: 1413.683874 from(707.849213,2+)
Pr-DAVTYTEHAKcrR H4K77cr
Monoisotopic mass of neutral peptide Mr(calc): 1376.70 Variable modifications: N-term : Propionyl:13C(3) (N-term) K10 : Dimethyl (K) Ions Score: 57 Expect: 5.9e-005
MS/MS Fragmentation of DAVTYTEHAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 13738: 1376.664954 from(689.339753,2+)
Pr-DAVTYTEHAKme2R H4K77me2
Monoisotopic mass of neutral peptide Mr(calc): 1753.97 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K13 : Acetyl (K) Ions Score: 45 Expect: 0.00098
MS/MS Fragmentation of KTVTAMDVVYALKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 23128: 1754.007234 from(878.010893,2+)
Pr-KprTVTAMDVVYALKacR H4K79ac
Monoisotopic mass of neutral peptide Mr(calc): 1060.60 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K6 : Acetyl (K) Ions Score: 53 Expect: 0.00013
MS/MS Fragmentation of KSTGGKAPRFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 8527: 1060.595124 from(531.304838,2+)
Pr-KprSTGGKacAPR H3K14ac
Monoisotopic mass of neutral peptide Mr(calc): 1125.65 Variable modifications: N-term : Propionyl (N-term) K1 : Acetyl (K) K6 : Acetyl (K) Ions Score: 46 Expect: 0.00089
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 14486: 1125.647494 from(563.831023,2+)
Pr-KacQLATKacAAR H3K18ac, K23ac
Pr-KprQLATKacAAR H3K23ac
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 10716: 1145.683264 from(573.848908,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1145.69 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K6 : Acetyl (K) Ions Score: 74 Expect: 5.5e-007
Monoisotopic mass of neutral peptide Mr(calc): 1165.68 Variable modifications: N-term : Propionyl (N-term) K1 : Propionyl (K) K6 : Crotonoyl (K)Ions Score: 48
Expect: 0.00041
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 20231: 1165.678994 from(583.846773,2+)
Pr-KprQLATKcrAAR H3K23cr
Monoisotopic mass of neutral peptide Mr(calc): 1070.60 Variable modifications: N-term : Propionyl (N-term) K1 : Dimethyl (K) K10 : Propionyl (K) Ions Score: 46 Expect: 0.0005
MS/MS Fragmentation of KSAPSTGGVKFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 12259: 1070.593544 from(536.304048,2+)
Pr-Kme2SAPSTGGVKpr H3K27me2
Monoisotopic mass of neutral peptide Mr(calc): 1090.60 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Acetyl (K) K10 : Propionyl:13C(3) (K)Ions Score: 46 Expect: 0.00025
MS/MS Fragmentation of KSAPSTGGVKFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 13085: 1090.628694 from(546.321623,2+)
Pr-KacSAPSTGGVKpr H3K27ac
Monoisotopic mass of neutral peptide Mr(calc): 1507.84 Variable modifications: N-term : Propionyl (N-term) K6 : Crotonoyl (K) Ions Score: 49 Expect: 0.00035
MS/MS Fragmentation of VTIMPKDIQLARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 24307: 1507.840244 from(754.927398,2+)
Pr-VTIMPKcrDIQLAR H3K122cr
Monoisotopic mass of neutral peptide Mr(calc): 1661.90 Variable modifications: N-term : Propionyl (N-term) K3 : Acetyl (K) K14 : Propionyl (K) Ions Score: 59 Expect: 3.9e-005
MS/MS Fragmentation of VLKQVHPDTGISSKFound in gi|30061387, histone H2B type 1-H [Mus musculus]
Match to Query 29675: 1661.934114 from(831.974333,2+)
Pr-R-VLKacQVHPDTGISSKpr H2BK46ac
Monoisotopic mass of neutral peptide Mr(calc): 1812.85 Variable modifications: N-term : Propionyl (N-term) R15 : Methyl (R)Ions Score: 72
Expect: 1.7e-006
MS/MS Fragmentation of AMGIMNSFVNDIFERFound in gi|68226433, histone H2B type 2-B [Mus musculus]
Match to Query 19777: 1812.850134 from(907.432343,2+)
Pr-AMGIMNSFVNDIFERme H2BR72me
Monoisotopic mass of neutral peptide Mr(calc): 1916.06 Variable modifications: N-term : Propionyl (N-term) K9 : Acetyl (K) K17 : Propionyl (K) Ions Score: 58 Expect: 9.3e-005
MS/MS Fragmentation of LLLPGELAKHAVSEGTKFound in gi|68226433, histone H2B type 2-B [Mus musculus]
Match to Query 21276: 1916.095494 from(959.055023,2+)
Pr-LLLPGELAKacHAVSEGTKpr H2BK108ac
Monoisotopic mass of neutral peptide Mr(calc): 1889.06 Variable modifications: N-term : Propionyl:13C(3) (N-term) K9 : Crotonoyl (K) Ions Score: 73 Expect: 2.5e-006
MS/MS Fragmentation of LLLPGELAKHAVSEGTKFound in gi|30089704, histone H2B type 1-K [Mus musculus]
Match to Query 22135: 1889.056794 from(945.535673,2+)
Pr-LLLPGELAKcrHAVSEGTK H2B108cr
Monoisotopic mass of neutral peptide Mr(calc): 1406.74 Variable modifications: N-term : Propionyl (N-term) K8 : Crotonoyl (K) K12 : Propionyl (K) Ions Score: 46 Expect: 0.00092
MS/MS Fragmentation of HAVSEGTKAVTKFound in gi|30061387, histone H2B type 1-H [Mus musculus]
Match to Query 26107: 1406.736974 from(704.375763,2+)
Pr-HAVSEGTKcrAVTKpr H2BK116cr
Monoisotopic mass of neutral peptide Mr(calc): 1386.74 Variable modifications: N-term : Propionyl:13C(3) (N-term) K8 : Acetyl (K) K12 : Propionyl:13C(3) (K) Ions Score: 79 Expect: 3e-007
MS/MS Fragmentation of HAVSEGTKAVTKFound in gi|13386452, histone H2B type 3-A [Mus musculus]
Match to Query 22240: 1386.776764 from(694.395658,2+)
Pr-HAVSEGTKacAVTKpr H2BK116ac
Monoisotopic mass of neutral peptide Mr(calc): 919.56 Variable modifications: N-term : Propionyl (N-term) R7 : Methyl (R) Ions Score: 38 Expect: 0.0008
MS/MS Fragmentation of HLQLAIRFound in gi|817939, histone H2A [Mus musculus domesticus]
Match to Query 5038: 919.556554 from(460.785553,2+)
Pr-HLQLAIRme H2AR96me
Monoisotopic mass of neutral peptide Mr(calc): 1300.68 Variable modifications: N-term : Propionyl (N-term) K1 : Propionyl (K) K7 : Crotonoyl (K) K9 : Propionyl (K) Ions Score: 60 Expect: 1.4e-005
MS/MS Fragmentation of KTESHHKAKFound in gi|817939, histone H2A [Mus musculus domesticus]
Match to Query 23609: 1300.675324 from(651.344938,2+)
Pr-KprTESHHKcrAKpr H2AK133cr
Montellier et al. Figure S6
A
Male Th2b+/+
XFemale Th2b+/+
Male TH2B-lessX
Female TH2B-less
B
Male Th2b+/+
XFemale Th2b+/+
Male Th2b+/+
XFemale Th2b+/tag
Male Th2b+/tag
XFemale Th2b+/+