Supplemental material Selenter Westensee See · 2014. 5. 19. · ! 1 Supplemental material Fig. S1: 1.5 % Agarosegels of PCR-products of hypD of the samples taken from the two different

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Supplemental material

Fig. S1: 1.5 % Agarosegels of PCR-products of hypD of the samples taken from the two different lakes. PCR samples with the DNA isolated from the 10 µm filters and the 0.2 µm filters were loaded on the gel. The band at about 250 bp is specific to the hypD amplicon. The diffuse band with a lower molecular mass is due to formation of primer dimers.

250

500 750

1000 1500

10 0.2 10 0.2

Selenter See

Westensee

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Table S1: The NiFe-hydrogenases found in the completely sequenced genomes with the closest BLAST hits of the hypD environmental libraries. The different hydrogenase genes were assigned to the different NiFe-hydrogenase classes according to Vignais and Billoud 2007. The numbers given in parenthesis are the percentage of genomes with the respective hydrogenase group.

The following NiFe-hydrogenase groups are distinguished: Group 1: membrane bound H2 uptake hydrogenases (some are oxygen tolerant) Group 2a: cyanobacterial uptake hydrogenases Group 2b: H2-sensing hydrogenases Group 3a: F420-reducing hydrogenases Group 3b: Bifunctional (NADP) hydrogenases (formerly also called sulfhydrogenases) Group 3c: Methylviologen-reducing hydrogenases Group 3d: Bidirectional NAD(P)-linked hydrogenases Group 4: Membrane-bound H2 evolving hydrogenases Group 5: Newly discovered group of oxygen tolerant high affinity hydrogenases (Constant et al 2010, Schafer et al 2013). hoxV/hupK: maturation gene known to be essential for assembly of oxygen tolerant group 1 hydrogenases (Ludwig et al 2009).

Bacterial Order NiFe-hydrogenase group hoxV

hupK

Number of

genomes 1 2a 2b 3a 3b 3c 3d 4 5

Rhizobiales 16 (80)

5 (25)

17 (85) 3

(15) 1 (5)

2 (10)

12 (60) 20

Legionellales 8 (100) 8

Burkholderiales 11 (79) 7

(50) 5 (36) 1

(7) 5

(35) 14

Bacteroidetes 2 (22)

3 (33) 2

(22) 2 (22) 9

Acidobacteria 3 (75) 1

(25) 2 (50) 4

Actinobacteria 2 (6)

5 (15) 12

(35) 2 (6) 32

(94) 34

Chloroflexi 3 (38)

3 (38) 1

(12) 6 (75) 2

(25) 8

Cyanobacteria 41 (46) 1

(1) 72 (80) 90

Rhodobacterales 7 (70) 10

(100) 1 (10) 1

(10) 1 (10) 10

Total 44 (23)

57 (29)

34 (18)

0 (0)

28 (14)

0 (0)

87 (44)

1 (0.5)

41 (21)

18 (9.5) 197

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Constant, P., S.P. Chowdhury, J. Pratscher, and Conrad, R. 2010. Streptomycetes contributing to atmospheric molecular hydrogen soil uptake are widespread and encode a putative high-affinity NiFe -hydrogenase. Environ. Microbiol. 12: 821-829. Ludwig, M., T. Schubert, I. Zebger, N. Wisitruangsakul, M. Saggu, A. Strack, O., Lenz, P. Hildebrandt, and B. Friedrich. 2009. Concerted Action of Two Novel Auxiliary Proteins in Assembly of the Active Site in a Membrane-bound NiFe Hydrogenase. J. Biol. Chem. 284: 2159-2168. Schafer, C., B. Friedrich, and O. Lenz. 2013. Novel, Oxygen-Insensitive Group 5 NiFe -Hydrogenase in Ralstonia eutropha. Appl. Environ. Microbiol. 79: 5137-5145. Vignais P.M. and B. Billoud. 2007. Occurrence, classification, and biological function of hydrogenases: An overview. Chem. Rev. 107: 4206-4272.

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HoreH168 1 -------MKCVDELSDRKIIS------GIVSKIKAIKLSGKVKIMEVCGTHTMSIFKNGI Ccell 1 --------------------------------MKSLEIDREIRIMEICGTHTTTILKSGI Mthe 1 -------MSPLDRFCDTELSRKLMGRVAALADEVAERLGRPAVFIEVCGTHTVAISRLGL PtheSI 1 ---------MQNSRLELNFGREILKMLPSLAGAAARRLGRTPVIMEVCGTHTMAIARSGL Tery 1 -------MKYVKEFRDPDKAQGLVREIAQLSQKLGYTTEHPLKIMEVCGGHTHSIFKYGI L8106 1 -------MKYVDEFRDPQKLQALMGEINRLTLILERTPEHPLKIMEVCGGHTHSIFKYGI Mcht 1 -------MKYVDEFRDPQKAKALLREIQTLTTQFGKTASQPLKVMEVCGGHTHSIFKYGL RTM1035 1 -------MKYADEFRDPKAAQALLARIAAVTDEIGATAENPTRIMEVCGGHTHAIFRYGL Sste 1 -------MKHVQEFRDPKPAKALIARIGAVLDRIGATAEKPVHIMEICGGHTHAIFRYGL Sala 1 -------MKYVDEFRDPVRAGVLLKEIEALTAQIMAGRDRPVVIMEVCGGHTHSIFRYGI Nmob 1 -------MKYVDEFRDPDKARSLLRAIEGLVADIEICRRRPLQIMEVCGGHTHSIFKYGL Xaut 1 -------MRYVDEFRDPALASALVKEIGAISVRLERGRDRPFAIMEVCGGHTHAIFRYGL GeoMC1 1 ----MTMMKYVDEFRDPELIHKISEEIHRLVDPN-----YHYRFMEVCGGHTFSIFRFGL Sdys 1 -------MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGL Ecol073 1 -------MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGL VangS14 1 ------MSFDLKLLYQGFRDADTVLSLAEQIKAIAPQLPEPLKVMEVCGGHTHTIMKYGL PSKA34 1 ------MSFELKQLYQGFRDADTVLSLAEQIKAIAPQLPEPLKVMEVCGGHTHTIMKYGL SMR-4 1 -------MLDLKQLYQGFRDPDTIASFAEDIAKCARTLKEPINIMEVCGGHTHTIMKYGL SOS195 1 -------MLELKHLYQGFRDPQTIANLAREIELTAKLVKQPINIMEVCGGHTHTIMKYGL Npro 1 -----MSDLTLKDLYFKFRDPTAMKTLAELIRKEAKGLKEPMRVMEICGGHTHTIMKYGI OMED92 1 -----------MKYVDEYRDGAIARDIAARIRTEARSD-REYNFMEFCGGHTHAIFRYGI Hche 1 -----------MKYIDEFRNGDYAAEVARRIHAEADPE-RRYRFMEFCGGHTHTLFRYGI Aaeol 1 ------MRVEEISIFEAYKDKERVLKLSEKIKKLSEKIGRTVNIMEFCGGHTHAIMKYGI Bact3_1_19 1 -------MRFVDEYRSN-------EKVQTLLEAIRKKVTRPWYIMEICGGQTHAIARYRL Bact2_1_7 1 -------MRFVDEYRSN-------EKVQTLLEAIRKKVTRPWYIMEICGGQTHAIARYRL C51142 1 -------MKFVDEYRSA-------DLAQKYVEEIRKSVTQPWTIMEICGGQTHSIVKYGI Cwat 1 -------MKFVDEYRNA-------DLAQKYVQEITQTITRPWTIMEICGGQTHSIVKYGI UCYN-A 1 -------MKFVDEYRDS-------ELIKIFTENIIKNVSKPWTIMEICGGQTHSIFKYGI S6803 1 -------MKYVDEYRDA-------QAVAHYRQAIAREITKPWTLMEICGGQTHSIVKYGL Amar 1 -------MKFVDEYRDP-------DLIKYWVREIDHLVTQPWTIMEICGGQTHTIVKHGI MMCS 1 -------MKYLDEFSDP-------QLARKLIEQIKSVTTRRWSIMEVCGGQTHSIIRHGI MJLS 1 -------MKYLDEFSDP-------QLARKLIEQIKSVTTRRWSIMEVCGGQTHSIIRHGI Mval 1 -------MKYLDEFSNP-------ELAARLIEQITSAATRRWSIMEVCGGQTHSIIRHGI Rery 1 -------MKYLDEFSDP-------ELAGKLLDQIRALATRRWAIMEVCGGQTHSIIRHGI Save 1 -------MKYIDEFQDP-------ELARRLLDDIHSTVTRPWALMEVCGGQTHTIIRHGI RRS-1 1 -------MKYLDEYRDP-------ELAQRLFAEIRRITTRPWAIMEVCGGQTHSIIRNGI Caur 1 -------MKYLDEFRDP-------ELARRMFDQIRRITTRPWAIMEVCGGQTHSIIRNGI Gaur 1 -------MKYLAEFRDG-------EIAQRIAAEIRQLTTQPWAIMEVCGGQTHSIMRNGI Msme 1 -------MKYLDEFRDP-------VAARTLVDRINRAATRTWTMMEVCGGQTHSIIRNGI Ctha 1 -------MKYLDEFRDP-------KVARRILSEIRSLVTRPWVIMEICGGQTHSIIKNGI Cfer 1 -------MKYIDEYRDP-------QLARNLLAAIHRVTTRPWTIMEICGGQTHSIIRNGI Susi 1 -------MKYLDEYRDD-------RIAKKVVDEIRRTVTRPWVIMEVCGGQTHSIVKYGI Scel 1 -------MRFIDEYRDA-------RRVGPYVEAIRRVVSRPWSIMEVCGGQTHAIVRFGL Pmar 1 -------MKYLDEYRDL-------AAAQRLLDEIRRVSTRSWTLMEVCGGQTHSLLRHGI Ppac 1 -------MKWVEEFRDP-------AAIRAASAAIAEAITQPWTIMEVCGGQTHAIVRWSL Oter 1 -------MKFVDEYRDA-------PAAHQLADAIARRVTRPWTLMEICGGQTHSILKFGI Vbac 1 -------MKHLSEYRNP-------DRVRKIAQAIAETVTRPWTIMEICGGQTHNIARFGL Fjoh 1 -------MKYLSEYRNP-------ELVKHYISEIHKITTKPWNIMEICGGQTHSLVKNGL Maeol 1 -----------MLQNVNDKTLIKKSILGIKKLADELNKD--VKIMHVCGSHEHTICKYGI Mjan 1 ------------MININDRALIKKAIDKINKLAEKIDK---LKIMHVCGSHEHTICKYGI Minf 1 -----------------MRELVKKVTSKINELAKEFDE---LKIMHVCGSHEHTICKYGL Paby 1 -----------MLEKFRDKEIAQKIVRKIHKEAKGFD---ELRFMHVCGTHEDTVTRAGI Pfur 1 -----------MLEKFGDKAVAQKILEKIKEEAKGIE---ELRFMHVCGTHEDTVTRSGI TkodaKOD1 1 --------MEEPFEAYRSREVAMKLVEKIREEAKTLDG--EIRIMHVCGTHEDTVTRHGI Aprof 1 ------------MLKLKDRKRAIKGLAEELKGLMEGEE--TITIMHLCGTHEDTVTRYNL Parse 1 MKREIAAIEQAFRRKTAVTSTLIRSIKKYSEELKARDPGYVYKIMDFCGTHEWTIVHFGL Pisl 1 ----MAALELAFRRKSKITGMLIQYIKKYAKELKKRDGDYVYKVMDFCGTHEWTIVHFGL Ihosp 1 -----------MTIPQELVQKTVEAIRGLAKEAVKKKNKEVIKIMHVCGTHEHTVTHNGL Figure S2: Alignment of HypD proteins. The motifs used for the generation of the degenerated primers are indicated.

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HoreH168 48 KSLLP----DSIELISGPGCPVCVTPDIYIDKALALAKKEN-----VIITTFADLLRVPG Ccell 29 RKLLP----PNIKLISGPGCPVCVTSQGYIDAAINLSEKEN-----LIITTFGDMLNVPG Mthe 54 RSLLA----GRVELRSGPGCPVCVTDHQEIDMMVDLARLPG-----VTLGTFGDMLRVPG PtheSI 52 KSVLA----GCIELRSGPGCPVCVTDQRDIDRIVALANLPG-----IAIATFGDMLRVPG Tery 54 QEALP----NNIELIHGPGCPVCIMPRGRLDDAIALAEKPN-----VIFTTFGDGMRVPG L8106 54 EELLP----DSIELIHGPGCPVCVMPRGRLDAAIALADIPN-----VIFTTFGDAMRVPG Mcht 54 EEILP----DTIELIHGPGCPVCVMPRGRLDDAIAIADYPN-----VILTTFGDAMRVPG RTM1035 54 DKLVP----EGLDFIHGPGCPVCVLPMARVDECVELAEDPR-----VIFTTFGDAMRVPG Sste 54 DKLVP----PGLEFIHGPGCPVCVLPMSRVDECIEIAERPE-----VIFTTFGDAMRVPG Sala 54 EKMLP----PEIEFAHGPGCPVCVLPMGRVDDCVELAMKPE-----VIFTTFGDAMRVPG Nmob 54 ESLLP----EAIEFVHGPGCPVCVLPVGRVDDGLVLAEHPS-----VIFTTFGDAMRVPG Xaut 54 ECLLP----DLVEFVHGPGCPVCVLPMGRVDDCVAIAEREE-----VIFATFGDAMRVPG GeoMC1 52 QHLLP----ENIELVHGPGCPVCVLPMGRMDDGLSIAQQPN-----VIFTAFGDVMRVPG Sdys 54 DQLLP----ENVEFIHGPGCPVCVLPMGRIDTCVEIASHPE-----VIFCTFGDAIRVPG Ecol073 54 DQLLP----ENVEFIHGPGCPVCVLPMGRIDTCVEIASHPE-----VIFCTFGDAMRVPG VangS14 55 KQLLP----ENIDFIHGPGCPVCVMPKERIDHAAALACQPN-----VILVTLGDMIRVPG PSKA34 55 KQLLP----ENIDFIHGPGCPVCVMPKERIDHAAALACQPN-----VILVTLGDMIRVPG SMR-4 54 LDLLP----ENIEFIHGPGCPVCVMPKERIDQAAVLAQQEN-----VILVTLGDMIRVPG SOS195 54 LDLLP----DNIEFIHGPGCPVCIMPKERIDQAAALASQEN-----VILVTLGDMIRVPG Npro 56 KQLLP----ENIEFIHGPGCPVCVMPKERIDHAIELAKQDG-----VILATLGDMIRVPG OMED92 49 PDLLP----DNVKMIHGPGCPVCVLPIPRLDAAIELAVTHE-----VILCSYGDMLRVPG Hche 49 PDLLP----ANVKMIHGPGCPVCVLPMARIDAAIELAVRHQ-----VVLCSYGDMLRVPG Aaeol 55 DQLLK----GVVRFIHGPGCPVCVMPMQRIDLAIELAKRKD-----VIMCSYGDLLRVPG Bact3_1_19 47 EEMLP----PDIQLLHGPGCPVCVTPVETIDYALKLATQPN-----VILASFGDMMRVPG Bact2_1_7 47 EEMLP----PDLQLLHGPGCPVCVTPVETIDYALKLATQPN-----VILASFGDMMRVPG C51142 47 DSLLP----PEITLIHGPGCPVCVTPIELIDCALKIASLSN-----VIFCSFGDMLRVPG Cwat 47 DRLLP----PEITLIHGPGCPVCVTPIELIDCALKIASLPN-----VIFCSFGDMLRVPG UCYN-A 47 DQLLP----PEITLIHGPGCPVCVTPIELIDYALIIANLPN-----IIFCSFGDMLRVPG S6803 47 DALLP----KNLTLIHGPGCPVCVTPMELIDQALWLAKQPE-----IIFCSFGDMLRVPG Amar 47 DQLLP----KAITLIHGPGCPVCVTPVALIDDAITLAKTPG-----VMLCSFGDMLRVPG MMCS 47 DQLLP----DQIEMIHGPGCPVCVTPLEIIDKALEIASRPE-----VIFCSFGDMLRVPG MJLS 47 DQLLP----DQIEMIHGPGCPVCVTPLEIIDKALEIASRPE-----VIFCSFGDMLRVPG Mval 47 DQLLP----DTVEMIHGPGCPVCVTPLEIIDKALDIASRPD-----VVFCSFGDMLRVPG Rery 47 DQLLP----QQIEMIHGPGCPVCVTPLEIIDKALDIAARPD-----VIFCSFGDMLRVPG Save 47 DQLLP----EQVELIHGPGCPVCVTPLEVIDKALEIASRPE-----VIFCSFGDMLRVPG RRS-1 47 DQLLP----EEIELIHGPGCPVCVTPLEIIDKALAIASRPN-----VIFCSFGDMLRVPG Caur 47 DQLLP----PEIELIHGPGCPVCVTPLEIIDKALAIAARPG-----VIFCSFGDMLRVPG Gaur 47 DQLLP----PEVELIHGPGCPVCVTPLEVIDKALAIAATPG-----VIFCSFGDMLRVPG Msme 47 DQLLE----GSVEFVHGPGCPVCVTPLEVIDRALEIAARDD-----VIFCSFGDMLRVPG Ctha 47 DQLLP----KEVELVHGPGCPVCVTPLELIDRALAIAARPN-----VIFTSFGDMLRVPG Cfer 47 DQLLP----DAIELVHGPGCPVCVTPLESIERALAIAAEKE-----VIFTSFGDMLRVPG Susi 47 DYLLP----PEIELVHGPGCPVCVTPLDMIDRAHAIARRPD-----VIFCSFGDMLRVPG Scel 47 DELLP----PEISLLHGPGCPVCVTPVELIDRALEIASRRE-----VTFCSFGDMLRVPG Pmar 47 AAELQ----GTVELIHGPGCPVCVTDQADIDFACQLAKRPD-----VMVTSFGDMLRVPG Ppac 47 HELLP----PELELVHGPGCPVCVTPGEVLDAARDLAVEGG-----VILATYADMLRVPG Oter 47 DELLP----RAVTLVHGPGCPVCVTPVELIDAAIALAGRPG-----VVLCSFGDMLRVPG Vbac 47 EELLP----KSISLAHGPGCPVCVTPVSLIDKSIAIARQPD-----VILCSFGDMLRVPG Fjoh 47 LDLLP----KNIRMIHGPGCPVCVTPVSLINKAIELMKQG------VVLCSFGDMIRVPG Maeol 48 RDVLP----NNITVIPGPGCPVCVTTQKEIDKAIYLAEKG------YTVATLGDMYRVPG Mjan 46 RDVLP----ENITVVPGPGCPVCVTTQKEIDTAIYLADNG------YVITTLGDMYRVPG Minf 41 RELLP----ENLLLIPGPGCPVCVTTQKEIDTAIYLSEN-------YVITTLGDLYRVPG Paby 47 RSLLP----ENVKIMSGPGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPT Pfur 47 RSLLP----ENVKIMSGPGCPVCITPVEDIVKMMEIMKVAREEREEIILTTFGDMYRIPT TkodaKOD1 51 RSLLP----ENVKVVSGPGCPVCITPVEDIVAMQLIMRKAREEGEEIILTTFGDMYKIPT Aprof 47 RSLLP----ENLKVVPGPGCPVCITPDTDLQRAFHLLEKE-----NVILTTFGDMARVPF Parse 61 RSLLKKAGVDNVELVAGPGCPVCVTPSYYIEQAIKLALEG------VVIYTYGDVYKLPA Pisl 57 RSVLEMAGVDNVELVAGPGCPVCVTPSYYLEEAIKLAFEG------VVIYTYGDVFRLPA Ihosp 50 RSLMP----PEVELIAGPGCPVCVTPAAAVDALIDLSLSG------VRVYAYGDVYKLPG __________ Position of Primer hypD-for1 and hypD-for2

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HoreH168 99 SS----------------------------------------------GTLQEMRVNRKN Ccell 80 SIPFEDKDGLLNNENSDNPSNIRNIIQDQYGDKGNTFIFKDINFNTKANSLMNQRAKGKD Mthe 105 SR----------------------------------------------SSLERERAGGAD PtheSI 103 TG----------------------------------------------SSLELERARGAC Tery 105 S----------------------------------------------QKSLLQAKAQGAD L8106 105 S----------------------------------------------QKSLLQAKAQGAD Mcht 105 S----------------------------------------------QKSLLQAKAVGAD RTM1035 105 S----------------------------------------------AKSLLQAKAEGAD Sste 105 T----------------------------------------------GKSLMQAKADGAD Sala 105 S----------------------------------------------RLSLLGAKAEGAD Nmob 105 S----------------------------------------------KKSLLQAKADGAD Xaut 105 S----------------------------------------------KKSLLQAKADGAD GeoMC1 103 K----------------------------------------------HGTPLELKARGAD Sdys 105 K----------------------------------------------QGSLLQAKARGAD Ecol073 105 K----------------------------------------------QGSLLQAKARGAD VangS14 106 S----------------------------------------------KGSLAQYRAKGCD PSKA34 106 S----------------------------------------------KGSLAQYRAKGCD SMR-4 105 S----------------------------------------------QGSLASFRAKGCD SOS195 105 S----------------------------------------------KGSLASFRAKGCD Npro 107 S----------------------------------------------KSSLAKVRAEGAD OMED92 100 SR---------------------------------------------KRSLLKAKSEGAD Hche 100 AH---------------------------------------------RTSLQKAKSQGAD Aaeol 106 SK---------------------------------------------RKSLLNVRAEGAD Bact3_1_19 98 SK----------------------------------------------EDLLSVKARGAD Bact2_1_7 98 SK----------------------------------------------EDLLSVKARGAD C51142 98 SE----------------------------------------------KDLLTIKASGGD Cwat 98 SE----------------------------------------------KDLLTIKASGGD UCYN-A 98 TK----------------------------------------------RDLLMVKASGGD S6803 98 SG----------------------------------------------ADLLSIKAQGGD Amar 98 TK----------------------------------------------GDLLSAKAQGGV MMCS 98 SE----------------------------------------------SDLFQIKSRGGD MJLS 98 SE----------------------------------------------SDLFQIKSRGGD Mval 98 SE----------------------------------------------KDLFRVKSEGGD Rery 98 SS----------------------------------------------SDLFRIKSAGGD Save 98 TG----------------------------------------------RDLFQVRSEGGD RRS-1 98 SA----------------------------------------------KDLFRVKSEGGD Caur 98 SR----------------------------------------------KDLFRVKSEGGD Gaur 98 SD----------------------------------------------RDLFRVKSEGGD Msme 98 SH----------------------------------------------QDLFSVKARGGD Ctha 98 SH----------------------------------------------KDLFMARSEGAD Cfer 98 SS----------------------------------------------RDLFMVRSEGGD Susi 98 SE----------------------------------------------GDLFQVRAAGGS Scel 98 SS----------------------------------------------DDLFAVKSRGGD Pmar 98 SQ----------------------------------------------GSLLDARTRGGQ Ppac 98 QRR--------------------------------------------TGDLLAARAAGAD Oter 98 SG----------------------------------------------SDLLTARARGAE Vbac 98 SR----------------------------------------------QDLFAARADGAD Fjoh 97 SS----------------------------------------------KSLLQAKAEGGD Maeol 98 SE----------------------------------------------KSLMQLQSEGAD Mjan 96 SE----------------------------------------------KSLMEKQSEGCD Minf 90 SE----------------------------------------------KSLFDVRAEGKD Paby 103 PM----------------------------------------------GSFADLKSEGFD Pfur 103 PI----------------------------------------------GSFADLKSQGYD TkodaKOD1 107 PM----------------------------------------------GSFADLKSEGFD Aprof 98 EG----------------------------------------------KSFFYYKSKGYD Parse 115 LRPVKG-----------------------------------------ARSLAEARALGGD Pisl 111 LQRVRG-----------------------------------------ARSLAEAKAMGAD Ihosp 100 TK----------------------------------------------GSLAVAKARGGD

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HoreH168 113 ITTVYSPLEAVKIAYNNPGQEVIFLAIGFETTIPAIALSIIKANQLG-----------LN Ccell 140 VRVIYSPLEALNIAKKNPNKEVVFLAIGFETTAPTIALTIEQAYQEN-----------IR Mthe 119 VRIFYSPLDAVAFAEANPEREVVFLGIGFETTAPAVAASLARARAKG-----------VK PtheSI 117 VEIFYSPLEAVAYAARNPGKEVVFLGVGFETTAPAIALSIAAAAGQK-----------LP Tery 119 IRMVYSPLDSLKIAKENPDKEIIFFGLGFETTAPSAALTILQAEAEG-----------VK L8106 119 IRMVYSPLDALPIAKQNPDKEVIFFGLGFETTAPSTAFTILQAEAEN-----------IT Mcht 119 IRMVYSPLDSLQIAKDNPDKEVVFFGLGFETTAPSTALTILQADAEG-----------LQ RTM1035 119 IRMVYSPLDALEIARRNPEREVVFFGLGFETTTPSTALSIQAAAREG-----------LA Sste 119 IRMVYSPLDALEIARRNPEREVVFFGLGFETTTPSTALSIQAAAREG-----------LA Sala 119 VRMVYSPLDALALARANPDREVVFFGLGFETTMPSTALTILQAQAEG-----------IG Nmob 119 VRMVYSPLDALGIAKANPGKEVVFFALGFETTIPSTAMTVLQAEREH-----------VA Xaut 119 VRMVYSPLDALTLAKRNPERQVVFFGLGFETTMPSTALTVLRAARER-----------VR GeoMC1 117 IRMVYSPLDALKIAMDHPDRQVVLFAIGFETTAPSTALTLMRARELD-----------VP Sdys 119 VRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQAKARD-----------VQ Ecol073 119 VRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQAKARD-----------VQ VangS14 120 IRPIYDPLDCLTIATENPDKEVVFFAIGFETSTPMTAVLVELAEQRQ-----------IN PSKA34 120 IRPIYDPLDCLAIATENPDKEVVFFAIGFETSTPMTAVLVELAEQRQ-----------IN SMR-4 119 IRPIYDPLDTLKIARDNPDKTVVFFAIGFETSTPMTAVLLQLAEQEK-----------LA SOS195 119 IRPIYDPLDTLKIARDNPDKTVVFFAIGFETSTPMTAILLQLAEHEK-----------LE Npro 121 VRPLHSTLDVLKIAKENPDKKVVFFAIGFETTTPMTAALMERAFKEG-----------IK OMED92 115 VRMVYSCMDALRIAQDNPGREVVFFAIGFETTTPPTALVIRQAQKLG-----------LK Hche 115 VRMLYSPADALRIALENPQRQVVFFAIGFETTTPPTAAVLLKAQELK-----------LR Aaeol 121 VRMVYSCLDTLKIAKENPDKKVIFFAIGFETTTPQTAVLIKKAKEEG-----------IK Bact3_1_19 112 IRMLYTPLDALSLAEENPDKEIVFFAIGFETTAPVHLMALKEAIRRG-----------LP Bact2_1_7 112 IRMLYTPLDALSLAEENPDKEIVFFAIGFETTAPVHLMALKEAIRRG-----------LP C51142 112 VRIVYSPLDSLKIAQENPDKEVVFFAVGFETTTPATAMAVYQAKQQG-----------IN Cwat 112 VRIVYSPLDSLKIAQENPDKEVVFFAVGFETTTPATAMAVYQADQQG-----------IE UCYN-A 112 VRIVYSPLDSLKIAQENPDKQIVFFAVGFETTAPATAMAVYQARHQN-----------IK S6803 112 VRIVYSPLDCLAIARENPNREVVFFGVGFETTAPATAMTLHQARAQG-----------IS Amar 112 VRIVYSSLDALKLAQANPDQQIVFFAVGFETTVPATAMAVYQAHQLG-----------IS MMCS 112 VRVVYSPLDALTVAKDNPDRQVVFFGIGFETTAPANAMTVYQAKRQG-----------IP MJLS 112 VRVVYSPLDALTVAKDNPDRQVVFFGIGFETTAPANAMTVYQAKRQG-----------IP Mval 112 VRVVYSPLDALTIAREHPDKQVVFFGIGFETTAPANAMTVYQARRLG-----------ID Rery 112 VRVVYSPLDALTIAKENPGRQVVFFGIGFETTAPANAMTVYQANRLG-----------IE Save 112 VRVVYSPLDALRIARQNPDREVVFFGIGFETTAPPNAMTVHQARRLG-----------IP RRS-1 112 VRIVYSPLDAVRLAQQHPDREVVFFGIGFETTAPANAMAVLQAHRLG-----------LR Caur 112 VRVVYSPLDAVKLAQQNPDREVVFFAIGFETTAPANAMAVYQAARLG-----------LK Gaur 112 VRVVYSPLDAVALARQHPNRQVVFFGIGFETTAPANAMAVHIAKLER-----------LH Msme 112 VRIVYSPLDATRVAADNPDKQVVFFGVGFETTAPANAMAVVHAKRLG-----------LK Ctha 112 VRTVYSPLEALKLAREHPEKEVVFFAVGFETTAPGNAMAVFQAKKEG-----------LK Cfer 112 VRIVFSPLDALQIARDNPQKEVVFFAVGFETTAPANAMAVWQAAREG-----------VE Susi 112 VRIVYSPMDCLNIAQQNPEKQVVFFAIGFETTAPANAMAVSRAKALG-----------LR Scel 112 VRVVYSPLDALALAERLPDREVVFFAVGFETTAPANAMAVFEARRRG-----------IR Pmar 112 VKIVYSPLDAVELARRQPEKQVVFFAVGFETTAPATALAVKQAAQYQ-----------LD Ppac 114 VRAVASPLDALRLAQAHPEREVVMLAVGFETTAPASAAAIEAAARGG-----------VA Oter 112 VRTVYSPLDAVALAQREPAREVVFFAVGFETTAPANAMAVLQAHRLK-----------LR Vbac 112 VRIVYSPLDALALAKRNPDKQVVFFAVGFETTAPANAMAVFQAAEKR-----------VS Fjoh 111 LRILYSPLEAVNIAAKNLDKEIVFFAVGFETTAPSNALAVLHAQKLG-----------LT Maeol 112 VKIVYGIGDAVKFAKENPDKKVVFVAIGFETTAPTTASELLSLNNYNNNEDNIKNNKTID Mjan 110 VRIVYSISEAVKMAKKERDKKFVFVAIGFETTAPTTGAELISLKNKD-----------VN Minf 104 VRIVYSITDAVRMAKREKDKNFLFLAIGFETTAPTTGAELINLN--------------ID Paby 117 VRVVYSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEG-----------LD Pfur 117 VRIVYSIYDSYKIAKENPDKLVVHFSPGFETTAAPTAGMLESIVEEG-----------LE TkodaKOD1 121 VRIVYGIFDTYRIAKENPDKTVVHFSPGFETTTAPAAGMLNVAAQEE-----------LE Aprof 112 VRIVYSVFDALEIAKTT-DKPVVFFAIGFETTMPSTA----VAVLDG-----------VE Parse 134 VRIVHSFLHAILDARKH-AKPSAFVGIGFETVAPGYSEAILKGLVP-------------N Pisl 130 VRLIHTFLHAAIDAKRH-NKPSLFLGIGFETVAPGYSEAILRGLVP-------------P Ihosp 114 VKVVYGFLDAIRDAKQY-GKEAVFFGVGFETTVVTVAGPIYDGRVP-------------K _______ Position of primer hypD-rev

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HoreH168 162 NFSILQSLKIMPPVLQELVLDKDLDVDG------FILPGHVSTISGLQPFEFLARDYHVP Ccell 189 NFSAFTSLKTMPEAIKGLISDKEINVDG------VICPGHVSTIIGENAFKFMSEELHMP Mthe 168 NFSLFSAHKLIPPALRAVLSDPELKLDG------LILPGHVSAVTGRRAFDFIVKEYGLP PtheSI 166 NYTVLSLHKLVPPVMETLLSDPELTVDG------FILPGHVSTITGRKAFDFISLKYGRP Tery 168 NFSIFSNHVLVVPALEALLSNPDLELQG------FVGPGHVSMVIGTQVYHIISEKYQKP L8106 168 NFSIFCNHVIVVPALEALLDNPDLELDG------FIGPGHVSMVIGSNPYQIIAEKYYKP Mcht 168 NFSMFCNHVLVIPALQALLDNPDLQLDA------FIGPGHVSMVIGTEPYHFIAQQYQKP RTM1035 168 NFSVFCNHITVPPPIKALLDDPYMRLDG------FVGPGHVSMVIGIHPYDFIARDYGKP Sste 168 NFSVFCNHITVPHPIRALLDDPHMVLDG------FVGPGHVSMVIGVHPYDFIAEDYGKP Sala 168 NFSLFCNHITIIPTIKAILDSPDMGIDG------FLGPGHVSMVIGTDPYDFIARDYHRP Nmob 168 NFSVFCNHITIIPTIKAILDSPELTLDG------FLGPGHVSMVIGTAPYQFICRHYRKP Xaut 168 NFSLFCNHITIIPTLRALLEQPDLGIDG------FIGPGHVSMVIGTSPYRFIAEEFHKP GeoMC1 166 NFSVFSNHVLIIPAIQAILDSPDMRIDG------FIGPGHVSSVIGAHPYEFIAKKYRKP Sdys 168 NFYFFCQHITLIPTLRSLLEQPDNGIDA------FLAPGHVSMVIGTDAYNFIASDFQRP Ecol073 168 NFYFFCQHITLIPTLRSLLEQPDNGIDA------FLAPGHVSMVIGTDAYNFIASDFHRP VangS14 169 NLYFHINHVLVPPAVDAVMADPAVKVNA------FIGPSHVSVIEGAKIYRPLVDKYNTP PSKA34 169 NLYFHINHVLVPPAVDAVMADPGVKVNA------FIGPSHVSVIEGAKIYRPLVDKYNTP SMR-4 168 NLLFHINHVLVPPAIDAVMHDPKARVNA------FIGPAHVSVISGAKIYRPAVEQYGTP SOS195 168 NLLFHINHVLVPPAIDAVMHDPKARVNA------FIGPAHVSVISGAKIYRSTVENYGTP Npro 170 NIYYHINHVLVPPPMRAIMDSGEAKINA------FIGPSHVSVIIGAKSYQFLVDSYNTP OMED92 164 NFSVYCNHVLTPAAISNILESPEVRELGVLPLDGFIGPAHVSTVIGSQPYHFFAEEYQKP Hche 164 NFSVFCNHVLTPPAITGILEP--AQQAGERIIEGLVGPGHVSVITGGGAYDSLARRYRTP Aaeol 170 NLFVVSNHVITPAAIQHILNAPEIREIGKVEIDAFIGPGHVSVIIGTKPYHYFAEEFQKP Bact3_1_19 161 NFSILTSLFTVPPAIEAILSDPESKVNG------FLTAGHVCAITGNRAYHRLAAHYKIP Bact2_1_7 161 NFSILTSLFTVPPAIEAILSDPESKVNG------FLTAGHVCAITGNRAYHRLAAHYKIP C51142 161 NFSLLVSHVLVPPAMEALLSSPNCVVQG------FLAAGHVCTVMGYEAYEPIAKKYQVP Cwat 161 NLSLLVSHVLVPPAMSALLSSPNCVVQG------FLAAGHVCTVMGYEAYKPIAQKYKVH UCYN-A 161 NFSLLVSHVLVPPAMKMLLSSSNCAIEG------FLAAGHVCTITGYKEYQSISEKYKIP S6803 161 NFSLLCAHVLVPPAMEALLGNPNSLVQG------FLAAGHVCTVTGERAYQHIAEKYQVP Amar 161 NFSVLVAHVLVPPAMEAILSAPNCLVQG------FLAAGHVCTVMGYTAYEAIATQYHTP MMCS 161 NFSLLVSHVLVPPAISAIMESPTCRVQA------FLAAGHVCSVMGTAEYPPLCEKYGIP MJLS 161 NFSLLVSHVLVPPAIAAIMESPTCRVQA------FLAAGHVCSVMGTAEYPPLCEKYGIP Mval 161 NFSLLVSHVLVPPAISAIMESPTCRVQA------FLAAGHVCAVMGTDEYPPLCDKYGIP Rery 161 NFSLLVSHVLVPPAMAAIMDSPECRVQA------FLAAGHVCSVMGTSEYPALAEKYKVP Save 161 NFSLLVSHVRVPPAIEAIMSAPNCRVQA------FLAAGHVCSVMGVGEYPELAERFRVP RRS-1 161 NFSMLVSHVLVPPAISAIMESPTNRVQG------FLAAGHVCSVMGTWQYQPLVERYRVP Caur 161 NFSLLVSHVLVPPAIRAIMESPSNRVQG------FLAAGHVCSVMGTDEYRPLVEQYRVP Gaur 161 NFSMLVSHVLVPPAIDAIMRSPTNRVQA------FLAAGHVCSVMGFWQYPALSSMHRVP Msme 161 NFSMLVSHVLVPPAMTAILSSPSNRVQG------FLAAGHVCSVMGTSEYGPLVEKFDVP Ctha 161 NFSLLVSHVLVPPAMEALLGSPQNRVQA------YLAAGHVCTVMGWEEYEPIAEKFHVP Cfer 161 NFSVLVSQVMVPPAMRAILSSPGNRVQG------FLAAGHVCAVMGYEEYEPVAQEFHVP Susi 161 NFSLLVSHVVVPPSMTGILQSPGNRVQG------FLGPGHVCAVMGWSEYEPIAARYGTP Scel 161 NFSLLVSHVLVPPAMEVILRAPDRRVDG------FLAAGHVCTVMGIDAYRPLAAEHRVP Pmar 161 NFSLIVSHVRVQPAMESLVESPENRVQA------FLAAGHVCTVMGYESYNRLVERYQLP Ppac 163 NFSVLASHVRVAPAMAMILAGEDNRVQG------FLAAGHVCAVVGTEDYPPLVERYGAP Oter 161 NFSLLISHVLVPPAIAAILSAPDNCVQG------FLAAGHVCTVMGLEEYHPLAAQFHVP Vbac 161 NFSILVSHVLVPPAIHALLSDPENRVQG------FLAAGHVCAITGESEYWPIAKKYGIP Fjoh 160 NFSLLVSHVLVPPAMEAILSDKFCTINA------FLGAGHVCTIVGLEEYYPIAEKYKIP Maeol 172 NFHILNCHRQTPPVMDFLLREGTVNMDG------FICPGHVSVITGLKPYYAPCEKYKAP Mjan 159 NFFILNCHRQTPPVMEFLLNEG-VYLDA------FICPGHVSTITGLKPYYGLCEKYKAP Minf 150 NFFILNCHRRTPPVMEFLLEDS--KIDA------FICPGHVSAIIGVKPYYPLVEKYRVP Paby 166 NFKIYSVHRLTPPAVEALLKAGT-VFHG------LIDPGHVSTIIGVRGWAYLTEKYNIP Pfur 166 NFKIYSVHRLTPPAVEALLNAGT-VFHG------LIDPGHVSTIIGVKGWAYLTEKFGIP TkodaKOD1 170 NFKIYSVHRLTPPAVEVLLKQGT-VFQG------LIAPGHVSTIIGVKGWEYLTEKYGIP Aprof 156 NFYVLSAHRFFIPAMEHLLSFDD-VRID-----GFICPGHVSTIVGVK----AYEHIKVP Parse 180 HLKLMSLVKLTPPAMFYTLEVVREKPTD-FPISGVIAPGHVSTIVGGKAWRPVAEQFEIP Pisl 176 NLKLMSLVKLTPPAMFYALDMVKEKPTD-YPISGVIAPGHVSTIIGGKAWAPVAEQYRVP Ihosp 160 NLKVLSAYLLTAPGLKRAIEAH--EATG-IPLDGIIAPGHVSSVIGANAWKYLPEEFGFP

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HoreH168 216 GVVAGFEPGDIILSIYTLCKMLKDNDIRVKNLYPRLVRLEGNKKAQELIKEIFTPVD-SY Ccell 243 SVIAGFEDRDVLIAVCLLVDMIRENKHELKNIYGSVVKLEGNKKAKELINKVLNVVD-SN Mthe 222 SVISGFDPMDILIALHELLLQLLRGEARVANKYTRVVREEGNLDARKRIAECFEEVD-VP PtheSI 220 AVVTGFEEIDILQSVCLLLKQISEGSARTVNGYKRLVREEGNKKAKEILAEYFKPAD-AF Tery 222 VVVSGFEPLDILQSIWMLLQQILEGRCEVENQYSRIVKEEGNRVALEAMKRVFEVRDTFE L8106 222 VVVSGFEPLDIVQSVWMLLQQLVDRRCEVENQYKRLVQPDGNPMALEAISKVFEVREEFE Mcht 222 LVVSGFEPLDILQSLWMILQQLIQGRCEVENQYDRIVKPEGNSVALNAMNTVFEVRQKFE RTM1035 222 IVVAGFEPLDLLQSVLMVLEQIRDGRAEVENQYARVVPEHGNPVSLAAIADVYEPRPSFE Sste 222 LVVAGFEPLDLLQSVLMVLEQIAEGRAEIENQYARVVPEHGNPVSLAAIADVYEARPSFE Sala 222 LVVAGFEPLDVLHSVWMVLRQMAEGRAEIENQYDRIVPRHGNEASLAAVTEVFELREFFE Nmob 222 LVVAGFEPLDILQSIWMLLRQLAEGRCEVENQYRRVVADAGNDPALRAVQEVFELREFFE Xaut 222 LVVAGFEPIDLLQSLLMVLRQIEAGTARIENQYARVVPEHGNPQALKAVEEVYEPRETFE GeoMC1 220 IVISGFEPLDLLQSIFMLVKQMREGRFEVENQYSRVVPSEGNISALDVMKEVFEVRSLFE Sdys 222 LVVAGFEPLDLLQGVVVLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCVNGDSE Ecol073 222 LVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCVNGDSE VangS14 223 VVVAGFEPVDVMESILRITKQKIAGEPELDVQYTRAVTEEGNTVAQQAVKRCFDVRDSFR PSKA34 223 VVVAGFEPVDVMESILRITKQKIAGEPELDVQYTRAVTEEGNMIAQQAVKRCFDVRDSFR SMR-4 222 VVVSGFEPVDVMESILRIVRQKVAGTALLDIQYSRAVSEEGNLAAQAKVDQFFQTRPQFR SOS195 222 VVVAGFEPVDVMESILRIVRQKAAGKAELDIQYSRAVSQDGNLTAQAKVNQFFNIRPQFR Npro 224 VVVAGFEPVDVMESILMLVRQKLKGEPKVEIQYKRATTWDGNVLAQKMIDKYMDVRDTFR OMED92 224 VVIAGFEPLDVLQAILMLVRQINEGRAEVENEFARAVTYEGNPQAKSVVADTFELRRAFE Hche 222 IVIAGFEPLDILHSILLLVRQCNAGRYDVENQYSRAVTASGNAKAQSMAARTMTLRDSFE Aaeol 230 VVIAGFEPLNVLKAVYLILKQIYKGRAEVKNNYTRFVTYKGNLKAQNLVAELFELRKEFE Bact3_1_19 215 MVVTGFEPVDLLYGIYRCVSQLEAGSYEVENAYKRAVPEQGNAAARQLIDEMLEPCD-QD Bact2_1_7 215 MVVTGFEPVDLLYGIYRCVSQLEAGSYEVENAYKRAVPEQGNAAARQLMDEMLEPCD-QD C51142 215 IVVTGFEPIDVLQGIYHCIQQLETGKFDCENQYLRSVKTQGNSLAQQVIQEVFEVVP-RC Cwat 215 IVITGFEPIDVLQGIYHCIQQLETGKYDCENQYIRSVKSQGNFLAQQLIQEIFEVVP-RR UCYN-A 215 VIVTGFEPLDILKGIYQCILQLEAGEFNCKNQYSRSVSIDGNVLATKLVQEIFMTVS-RC S6803 215 IVITGFEPVDIMQGIFACVRQLESGQFTCNNQYRRSVQPQGNAHAQKIIDQVFEPVD-RH Amar 215 IVVTGFEPVDILQGIYRCIHQLEVGKAVVENQYSRVVQSNGNSEAQRLMADVFVTSD-RQ MMCS 215 IVVTGFEPLDILEGIRRTVVQLEAGRHELENAYPRAVRAEGNPAAKTMLADVFEVTD-RS MJLS 215 IVVTGFEPLDILEGIRRTVVQLEAGRHELENAYPRAVRAEGNPAAKTMLADVFEVTD-RS Mval 215 IVVTGFEPLDILEGIRRTVVQLESGRHELENAYPRAVREAGNPAAKAMLLDVFELTD-RA Rery 215 IVVTGFEPLDLLEGIRKTVLQLESGRYEVENAYARAVTAQGNTAAKAMLEDVFAVSD-RT Save 215 IVVTGFEPLDILEGVRRTVLQLERGEHTVDNAYARAVRPEGNPAAQAMLEDVFEVTD-RA RRS-1 215 IVVTGFEPLDVLEGIRRVILQLESGRAELDNAYERAVRSEGNVAAQQVLSEVFEVTD-RA Caur 215 IVVTGFEPLDVLEGIRRVILQLEQGKAELDNAYERAVRPEGNVAAKQMLADVFTVTD-RA Gaur 215 IVITGFEPLDVLEGIRRTIAQLEHGQHVVENAYARVVTFDGNRPAQELLERVFTVSD-RA Msme 215 IVVTGFEPLDLLEGVRQLVDLLEAGTPELRNAYSRAVTAEGNVFAQQTLSEVFTVTD-RQ Ctha 215 IVPTGFEPVDILEGILQVLKQLESGEAKVENQYLRAVKREGNPTARKILSEVFEITD-RK Cfer 215 IVPTGFEPVDLLAGILATVEQLEVGRAEVVNRYARVVSREGNRAATEIIRNVFEVID-RQ Susi 215 IVITGFEPVDLLEGILLTVRQLEQGRAEVENQYPRAVSREGNVAARRMIAEVFDVAD-RS Scel 215 IVVTGFEPLDILQGIFMCVRQLEEGRADVENQYARSVREAGNEAAQRMLREVFEIVP-RT Pmar 215 VVVTGFEPLDLLEGILACVRQLEAGETRLENCYSRAVQMAGNQSAQDLVQEIYQISD-AR Ppac 217 IVVTGFEPLDLLAGVLRCVRALEAGRPRLEVQYRRAVRPEGNPVARAALERVFEVVD-AP Oter 215 IVATGFEPVDILRGVLDCIEQLESGRAEVTNAYGRVVREAGNPHAQAVMREVFIPCD-RN Vbac 215 IVVTGFEPVDIMLGLQRCITLLEEGRAVVENQYARIVPPDGNAPALRLIREIFEVSD-RE Fjoh 214 IVVSGFEPADMVQAIYHTVLQLEQGKHEVENQYTRLVKEEGNLRAKEVVNTIFTIGT-QE Maeol 226 MVVAGFEPIDVLMSIFLILKQMINEEAKVENAYKRGVREEGNIVAQKIINKVFEPCD-VA Mjan 212 MVVAGFEPIDVLMAIIMILKQVISGEAKVENEYIRAVKPEGNVLAQKIINEVFESID-VP Minf 202 MVIAGFEPMDILISIYMILKQLNENNIRVENEYDRVVKEEGNVVAQKIIEKVFTPSD-KR Paby 219 QVVAGFEPVDVLLGILILIRMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKD-AK Pfur 219 QVVAGFEPVDVLLGILILIRLVKRGEAKIINEYNRVVKWEGNVKAQELIWKYFEVKD-AK TkodaKOD1 223 QVVAGFEPNDVLMAILMLIRMYKEGEARIINEYERAVKYEGNVVAQKMIDKFFEVVD-AK Aprof 206 QVIAGFEPEDVLLALCMLVKAIKNGENCLLNEYTRAVRYEGNVKAQKVMNEVFDRDD-WE Parse 239 VVVAGFEPNDVLTAVAEILRQLAKGEHKVVIEYTRAVTWEGDLKAQSSIRTVFETVD-SA Pisl 235 VVVAGFEPNDVLTAIAEILRQLKNGEHKVVIEYVRAVKWEGDLKAQSSIHTVFETVD-TA Ihosp 217 TVVAGFEAYDYLLAVAEILRQIVRGEAKLVNEYSRVVRPEGNVTAWRKVMEVFDVVD-AY

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HoreH168 275 WRGLGTITGSGLKLRKVYHRYSAEKKFQFGDT---------------QYRPGKGCICGEI Ccell 302 WRGLGEIKNSGLAISDKYKAFDAVIKFNIKVQ---------------EALSASGCICGSI Mthe 281 WRGLGVIPESGLSLRETFRDFDARFKLNIEPA-------------PPTPLYPPGCACKEI PtheSI 279 WRGFGLVPQSGLAIKEKYSRFDASAKFALD---------------TPETQPPKGCACGDI Tery 282 WRGLGEIPESGLKITDAYSCFDAEAKFCLPN---------------KKVADAKACQCGEI L8106 282 WRGLGDIAQSGLKINSNYAQFDAEIKFDVSD---------------VKVPDAAACQCGEI Mcht 282 WRGLGDIPNSGFKIRPKYAQFDAEVKFTLPN---------------RQVADHKACQCGEI RTM1035 282 WRGLGEIDASGLRIRPAYAAYDAEVKFGVGYGAER-----------RTVPEPEGCACGAV Sste 282 WRGLGEIDASGLRIREAYRAWDAEEKFGIGYAAGA-----------RSVAEPEGCMCGAV Sala 282 WRGLGSIDHSGVQIREAYAAWDAERKFAVAS---------------PSIPDPKACQCGEV Nmob 282 WRGLGSIDHSGVCIRDAYAAFDAERRFSVPN---------------LKIADPKSCQCGEV Xaut 282 WRGLGSIAGSGVRLRAPYAAFDAEKRFAVPD---------------VKVADPSSCRCGEV GeoMC1 280 WRGLGTIPQSALKLRPEFAKWDAEVRFQVEG---------------ARVNDPQMAQCGEV Sdys 282 WRGLGVIESSGVHLTPDYQRFDAEAHFRPAP---------------QQVCDDPRARCGEV Ecol073 282 WRGLGVIESSGVHLTPDYQRFDAETHFRPAP---------------QQVCDDPRARCGEV VangS14 283 WRGLGPIANSALKLNAAYQHRDAEIRFADCLPM-------------TPIDDHKACQCGDI PSKA34 283 WRGLGPIANSALKLNAAYQHRDAEIIFADCLPM-------------TPIDDHKACQCGDI SMR-4 282 WRGLGPIPNSALELRPEYAHRDAEKYFKDRLPV-------------KEIDDHKACQCGDI SOS195 282 WRGLGPIPNSALQLKPEYAHRDAELRFKDRLPV-------------KEIDDHKACQCGDI Npro 284 WRGLGDIPKSALKLRDEYAEFDAEKVFADILPN-------------EPIDDHKLCICGQI OMED92 284 WRGLGTVPYSALRIKAEFAEFDAEKRFTVDYK---------------STRENKACECGAI Hche 282 WRGLGWMPHSALRIRPEFADYDAERRFELSPS---------------EARENKACECPAV Aaeol 290 WRGLGTIPYSALKIRKEYEEFDAEKVFEVELP---------------EPKEHPACICGKV Bact3_1_19 274 WRGIGIIPSSGLQLRSEYKRYSSRMRFQLCPE---------------ENQIASECIAGLI Bact2_1_7 274 WRGIGIIPSSGLQLRSEYKRYSSRMRFQLCPE---------------ENRIASECIAGLI C51142 274 WRGIGKIEESGLGLRPEYALYDALKRFEA------------ELSSLTPSTAPEVCISGQI Cwat 274 WRGIGEIRESGLGLRPEYSLYDALKRFES------------PLSSLTPSTAPQVCISGEI UCYN-A 274 WRGIGEIPESGLSLRSEYQEYDALNRFQI------------FVDKPIP-EYPQICISGDI S6803 274 WRGLGLIPASGLGLRPAFAPWDAAVKFAN------------LLQTMAPTMGETVCISGEI Amar 274 WRGLTSIPNSGLKLRPRYAYLDAQALLPANS----------STSDCVAPAPESDCISGKI MMCS 274 WRGIGMIPQSGWRLSPAYRDFDAEYRFAVT---------------GIHTDESTLCRSGEV MJLS 274 WRGIGMIPQSGWRLSPAYRDFDAEYRFAVT---------------GIHTDESTLCRSGEV Mval 274 WRGIGVIPGSGWRLSHAYRDYDAEQRFAVS---------------DIHTEESAVCRSGEV Rery 274 WRGIGSIPDSGWQLAPKYAYFDAEERFSVT---------------DIHTDESSVCRSGEV Save 274 WRGIGVIPDSGWRLSPKYRDHDAEYRFSVD---------------GIQTREPAECRSGEV RRS-1 274 WRGIGVIPQSGWRLRDAYRAYDAETRFAVS---------------DIQTRESPICRSGEV Caur 274 WRGIGVIPRSGWKLSERYAAFDAEQRFDVS---------------DIQTTESALCRSGEV Gaur 274 WRGIGVIPNSGWRLSEAYRDYDAEYRFDVT---------------GIHTQESALCRSGEV Msme 274 WRGIGMIPRSGWTLSPQYAEFDAELRFGVG---------------HLQVAESAECRSGEV Ctha 274 WRGIGEIPKSGYRLREAYAAHDAEKKFAVG---------------DLETEESELCKSGII Cfer 274 WRGIGMIPASGFGLRDAYARFDAEKKFNVS---------------HICASESPLCMSGRV Susi 274 WRGIGIIPKSGYKLGYAFREYDADRIFEVQ---------------HIRTREPEICISGQV Scel 274 WRGIGPIARSGLGLRAPYADHDAERRWAPAGDLEPRASERRAPAGDLEPRASTDCKSGLV Pmar 274 WRGFDLIPAGGLSLREQWQQFDARVRFSDSSL----------PVILNS-----ECRSFDV Ppac 276 WRGLGEVPQGGLAIRPEFAAHDALRRFELRPD----------ERGVAAEGGQGSCRAADV Oter 274 WRGLGVIPHSGLTLREEFASFDALRRFDLAAV----------ATATAG-----ECIAGTI Vbac 274 WRGLGTIPQSGFTLRKSYQEFDATRRFSIP---------------DIPSRESPDCIAGKV Fjoh 273 WRGIGKIENSGLVLRENYKMYDANIKFSIETTN---------------SKTDNLCIAGQI Maeol 285 WRGFPVITDGGLKLREEYEKYDIHNIEELPVIE---------------EKINKACICSKI Mjan 271 WRGFPVVKNGGFGLREKYKKFDIYEHEDIPEIK---------------EKIPKGCICDKI Minf 261 WRGFPIIKGGGFELKEEFKKYDILEREEIPDIK---------------EKIPKGCICDKI Paby 278 WRALGIIPQSGLELKREWKDLEIRTLYNPDVPK-----------LPDLEKG---CLCGAI Pfur 278 WRALGVIPRSGLELKKEWKELEIRTYYNPEVPK-----------LPDLEKG---CLCGAV TkodaKOD1 282 WRALGVFPKSGLELRKEWKDFEIRSFYKVEVPKN----------LPDLEKG---CRCGAV Aprof 265 WRGLGVVRNSGAPLKKRYEDFDALKVFEDAFKDF----------EPREDKKKRLCRCGDV Parse 298 WRGIGYIPKSGLALRDEFKKHDALEHFGIPDLTP----------DTWRYDLPANCKCAEV Pisl 294 WRGIGYIPKSGLALREEFKQYDAFEYFGIPDLTP----------ERWRYDLPPGCKCAEV Ihosp 276 WRGIGVLPESGLAFKEKYKDYDAAAEYGIEIKP-------------TERELPPGCKCAEI

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HoreH168 320 LKGKQKPTDCRLFGIKCTPMKPEGPCMVSREGTCAAYYRYRGKVNL-------------- Ccell 347 LKGQKTPEDCKLFGKKCTPYSPLGVCMVSKEGTCGIHYKYLNDQ---------------- Mthe 328 LRGKLLPPECRLFSNVCTPVHPVGPCMVSSEGACAAYYRFERFSPVGGGI---------- PtheSI 324 LKGKLTAPDCPLFARACTPSSPAGPCMVSSEGACAAYYHYEWSEQDREVN---------- Tery 327 LKGVLKPWECKVFGTACTPENPIGTCMVSSEGACAAYYKYGRLFKKEKVKV--------- L8106 327 LKGVLKPWQCKVFGTACTPETPIGTCMVSSEGACAAYYKYGRLSTVIKANKPQRSKAKV- Mcht 327 LKGVMKPWQCKVFGTACTPENPIGTCMVSSEGACAAYYKYGRLSKVAKTAVGIN------ RTM1035 331 MTGRIKPVECPQFGRGCTPEMPLGALMVSSEGACAAYYQYGGARAVERAG---------- Sste 331 MTGRMKPVACPQFGTGCTPEMPLGALMVSSEGACAAYYQYGGARAAEPAE---------- Sala 327 LKGAIKPWQCKLFGRSCTPETPMGALMVSSEGACAAYYQYGGIDAVPEPAQ--------- Nmob 327 LKGVIKPRQCKVFGRACTPETPLGALMVSSEGACAAYYNYAGVQLGKVRRSRAAPDRGRA Xaut 327 LTGAVKPWACPEFGTGCTPETPLGALMVSSEGSCAAYYQYGGVRGEAA------------ GeoMC1 325 LKGVLKPHQCKLFGKECTPEHPVGALMVSSEGACAAYYHYVHLQDA-------------- Sdys 327 LTGKCKPHQCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA------------- Ecol073 327 LTGKCKPHQCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA------------- VangS14 330 LRGLAKPQDCKVFGRGCTPQTPLGSCMVSSEGACNAYFRYRGV----------------- PSKA34 330 LRGLAKPQDCKVFGRGCTPQTPLGSCMVSSEGACNAYFRYRGV----------------- SMR-4 329 LRGLAKPKDCKVFGRGCNPETPLGSCMVSSEGACNAYYRYQGIDSKEPHHG--------- SOS195 329 LRGLAKPKDCKVFGRGCNPETPLGSCMVSSEGACNAYYRYQGIDIKEPHHG--------- Npro 331 LRGLANPTDCKVFGTACTPSSPLGSCMVSDEGACNAYYRYAR------------------ OMED92 329 LRGVKSPRDCSLFGDPCTPENPLGSCMVSSEGACAAYYSYGRYQERTFATDQTHPAA--- Hche 327 LRGAKAPTDCKLFATVCTPDNPIGACMVSSEGACAAHYSYGHTR---------------- Aaeol 335 IRGVARPTDCKLFGTVCTPSNPLGSCMVSSEGACAAYYKYRRALVQ-------------- Bact3_1_19 319 MRGLRQPSDCPHFGKACHPTHPLGAPMVSSEGVCAAYYRYK------------------- Bact2_1_7 319 MRGLRQPSDCPHFGKACHPTHPLGAPMVSSEGVCAAYYRYK------------------- C51142 322 MQGYKKPNDCPAFGTQCTPENPLGAPMVSSEGACAAYYRYRHHQQTAPC----------- Cwat 322 MQGYKKPNDCPAFGTQCTPENPLGAPMVSSEGACAAYYRYRNHRQLVGQY---------- UCYN-A 321 MQGQKKPYDCPAFGSTCTPENPLGAPMVSSEGSCSAYYRYRGYSQI-------------- S6803 322 LQGQRKPSDCPAFGTICTPEQPLGAPMVSSEGACAAYYRYRQQLPEPVGAARV------- Amar 324 LQGVLKPPDCPAFGHQCTPEHPLGAPMVSSEGACAAYYRYRQFRV--------------- MMCS 319 LQGLIKPHECAAFGTVCTPRNPLGATMVSSEGACAAYYLYRRLEVSHA------------ MJLS 319 LQGLIKPHECAAFGTVCTPRNPLGATMVSSEGACAAYYLYRRLEVSHA------------ Mval 319 LQGLIKPHECAAFGTVCTPRNPLGATMVSSEGACAAYYLYRRLEVTNA------------ Rery 319 LQGLIKPHECSAFGKECTPRNPLGATMVSSEGACAAYYLYRRLELQEAGHAG-------- Save 319 LQGMLKPHECEAFGTVCTPRTPLGATMVSSEGACAAYYLYRRLDITTAAREASPVV---- RRS-1 319 LQGMLKPNQCAAFGKECTPRTPLGATMVSSEGACAAYYQYGRFIKAEEVGVAR------- Caur 319 LQGRLKPNQCPAFGKECTPRTPLGATMVSSEGACAAYYQYGRFIPVNQVGVTG------- Gaur 319 LQGTIKPPTCSAFGEQCTPRTPLGATMVSSEGACAAYFNYGRTVQLQRAGTSR------- Msme 319 LQGLLKPNECPAFGKTCTPRNPLGATMVSSEGACAAYYQFRRLEAAAHV----------- Ctha 319 LQGLLKPNQCPAFGAECTPQTPLGATMVSSEGACAAYYKYQRLTEKD------------- Cfer 319 LQGALKPDGCPAFAKECTPEHPLGATMVSSEGACAAYYNYHRNFTP-------------- Susi 319 LRGLKKPVDCPAFGAQCSPQTPLGATMVSAEGACAAYYNYGRHLVSIAAEA--------- Scel 334 LQGVIKPPDCPAFGVRCTPETPLGATMVSSEGACAAYYRYRRADPPRGSG---------- Pmar 319 MTGQLKPTECPHFGKQCTPETPLGAPMVSSEGACAAYYRYAGV----------------- Ppac 326 LRGRLKPLACPEFGRGCTPERPLGAPMVSSEGACAAYYRHRARHA--------------- Oter 319 LRGRAKPPACAAFGTRCTPEHPLGAPMVSSEGACAAYYRYRARSSP-------------- Vbac 319 LRGISTPMQCPLFETKCTPTRPQGAPMVSSEGACAAYYRYRRVQTAS------------- Fjoh 318 LTGNKRPNECPEFGKKCKPSFPLGAPMVSSEGACAAYFNYL------------------- Maeol 330 LRGEKLPTDCKLFGAPCNPMNPIGSCMVSDEGTCRIFYKYHNKL---------------- Mjan 316 LRGEKLPTDCPLFGTVCTPLNPVGSCMVSDEGTCRIFYKYRRI----------------- Minf 306 LRGEKLPTDCKLFGKSCNPLNPVGSCMVSEEGTCRIFFKYYRGV---------------- Paby 324 LRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIFYKYGALF---------------- Pfur 324 LRGLALPTQCQHFGKTCTPRHPVGPCMVSYEGTCHIFYKYGALM---------------- TkodaKOD1 329 LRGLALPTDCPLFGKTCTPRHPVGPCMVSYEGTCQIFYKYGVLF---------------- Aprof 315 LRGVATPLDCSLFMKACTPKNPIGACMVSFEGTCNIWARYWRG----------------- Parse 348 NLGKAKPTDCPLFMKACTPDRPIGPCMVSVEGTCAIWARFGGGGLAEEIAKEIGVF---- Pisl 344 NLGKAKPTDCPLFMKACTPDHPVGPCMVSVEGTCAIWARFGGGGLAVEVAKEIGLA---- Ihosp 323 NLGIAKPTDCPYFKTRCTPTTPIGPCMVSDEGTCSIWARFGGGGLVEELARLAGIE----

! 12

HoreH168 ---- Ccell ---- Mthe ---- PtheSI ---- Tery ---- L8106 ---- Mcht ---- RTM1035 ---- Sste ---- Sala ---- Nmob 387 RGMP Xaut ---- GeoMC1 ---- Sdys ---- Ecol073 ---- VangS14 ---- PSKA34 ---- SMR-4 ---- SOS195 ---- Npro ---- OMED92 ---- Hche ---- Aaeol ---- Bact3_1_19 ---- Bact2_1_7 ---- C51142 ---- Cwat ---- UCYN-A ---- S6803 ---- Amar ---- MMCS ---- MJLS ---- Mval ---- Rery ---- Save ---- RRS-1 ---- Caur ---- Gaur ---- Msme ---- Ctha ---- Cfer ---- Susi ---- Scel ---- Pmar ---- Ppac ---- Oter ---- Vbac ---- Fjoh ---- Maeol ---- Mjan ---- Minf ---- Paby ---- Pfur ---- TkodaKOD1 ---- Aprof ---- Parse ---- Pisl ---- Ihosp ---- !

! 13

Proteo-others-MagentococcusMC1 1 ------------------------------------------------------------ beta-Nitrospira_multi 1 ------------------------------------------------------------ beta-Thiobacillus_denitri 1 ------------------------------------------------------------ alpha-Acidiphilum 1 ------------------------------------------------------------ Thermococcales-Pyrococcus_fur 1 ---------------------------------------------------ATGCTTGAA Thermococcales-Pyrococcus_hori 1 ---------------------------------------------------ATGCTTGAG Thermococcales-Thermococcus_ko 1 ------------------------------------------ATGGAGGAGCCCTTTGAG Archaeoglobales-Archaeoglobus 1 ----------------------ATGAGGAGGAGGCAAAAAAGACTTGGGAGTTGCTGGAG Methanopyrales-Methanopyrus_ka 1 ------------------------------------------------------------ Methanomicrobiales-Methanospir 1 ------------------------------------------------------------ Sulfolobales-Metallosphaera_se 1 ---------------------------------ATGAGCGAGTACTTGTCATTGCTAAAG Thermoproteales-Pyrobaculum_is 1 ---------------------------------ATGGCCGCCCTCGAGCTGGCCTTTAGG Methanobacteriales-Methanother 1 ------------------------------------------------------------ Methanomicrobiales-Methanocull 1 ------------------------------------------------------------ Thermoproteales-Thermofilum_pe 1 ATGGAGGGTCAGGGGCTCGCTTTTCTGGAGAAGCTTGGCTACTTGGAGAAGGCTTTCAGG delta-Anaeromyxobacter 1 --------------------------------ATGTCGAACGACGATCTCTTCCGGCAGC delta-desulfovibrio_des 1 ------------------------------------------------------------ delta-geobacter_metalli 1 ------------------------------------------------------GTGAAA delta-Pelobacter_propion 1 ------------------------------------------------------ATGAAT delta-geobacterFRC32 1 ------------------------------------------------------ATGAAC proteo-others-Mariprofundus_fe 1 ------------------------------------------------------ATGAAA alpha-Stappia_agg 1 ------------------------------------------------------ATGAAA beta-Methylibium_petro 1 ------------------------------------------------------ATGAAG beta-Ralstonia_metall 1 ------------------------------------------------------ATGAAA beta-Rhodoferax_ferri 1 ------------------------------------------------------ATGAAA beta-dechloromonas_aro 1 ------------------------------------------------------ATGAAG beta-Azoarcus 1 ------------------------------------------------------ATGAAG beta-Burkholderia-viet 1 ------------------------------------------------------ATGAAG alpha-Magnetospirillum_mag 1 ------------------------------------------------------GTGAAA alpha-Bradyrhizobium_jap 1 ---------------------------------------------ATGCCGATCGTGAAA alpha-BradyrhizobiumBTAi1 1 ------------------------------------------------------ATGAAA alpha-Rhodopseudomonas_palBisA 1 ------------------------------------------------------ATGAAA alpha-Rhodopseudomonas_palCGA0 1 ------------------------------------------------------ATGAAA beta-Burkholderia_phy 1 ------------------------------------------------------GTGAAA alpha-Rhodospirillum_rub11170 1 ---------------------------------------------ATGACGACACCCGAG beta-Burkholderia_xeno 1 ------------------------------------------------------ATGAAA alpha-Sphingopyxis_alask 1 ------------------------------------------------------ATGAAG gamma-Shigella_flex 1 ------------------------------------------------------ATGCGT gamma-Shigella_sonn 1 ------------------------------------------------------ATGCGT gamma-Shigela_dys 1 ------------------------------------------------------ATGCGT gamma-EcoliK12 1 ------------------------------------------------------ATGCGT gamma-Ecoli_CFT073 1 ------------------------------------------------------ATGCGT gamma-salmonella_ent 1 ------------------------------------------------------ATGCGT gamma-salmonella_LT2 1 ------------------------------------------------------ATGCGT gamma-enterobacter_638 1 ------------------------------------------------------ATGCGC gamma-erwinia_caro 1 ------------------------------------------------------ATGCAT gamma-Yersinia_ber 1 ------------------------------------------------------ATGCGT gamma-Yersinia_int 1 ------------------------------------------------------ATGCGT gamma-Yersinia_fred 1 ------------------------------------------------------ATGCGC gamma-Yersinia_moll 1 ------------------------------------------------------ATGCGC gamma-Serratia_protea 1 ------------------------------------------------------ATGCAT alpha-Rhodobacter_sphaer 1 ------------------------------------------------------ATGAAA alpha-Rhodobacter_spaer17025 1 ------------------------------------------------------ATGAAG alpha-Roseovarius-2601 1 ------------------------------------------------------ATGAGA alpha-Paracoccus_deni 1 ------------------------------------------------------ATGAAA alpha-Xanhobacter_auto 1 ------------------------------------------------------ATGCGC actinobacteria-corynebacterium 1 ------------------------------------------------------ATGAAG Actinobacteria-RhodococcusRHA1 1 ------------------------------------------------------ATGAAG Chloroflexi-Acidobacteria 1 ------------------------------------------------------ATGAAG Chloroflexi-Solibacter_usi 1 ------------------------------------------------------ATGAAG delta-proteo_MMS1 1 ------------------------------------------------------GTGAAA Cyan-SynechococcusWH5701 1 ------------------------------------------------------ATGAGC Cyan-Synechocystis6803 1 ------------------------------------------------------ATGAAA chloroflexi-chloroflexus_agg 1 ------------------------------------------------------ATGAAA chloroflexi-chloroflexus_aur 1 ------------------------------------------------------ATGAAG Chloroflexi-Roseiflexus_cas 1 ------------------------------------------------------ATGAAG Chloroflexi-RoseiflexusRS-1 1 ------------------------------------------------------GTGAAG Actinobacteria-Mycobacterium_g 1 ------------------------------------------------------GTGAAG Actinobacteria-Mycobacterium_v 1 ------------------------------------------------------ATGAAG Actinobacteria-FrankiaCcI3 1 ------------------------------------------------------GTGAGG Actinobacteria-FrankiaACN14a 1 ------------------------------------------------------GTGAAA Actinobacteria-Streptomyces_av 1 ------------------------------------------------------GTGAAG Actinobacteria-Mycobacterium_s 1 ------------------------------------------------------ATGAAG Chlorobi-Pelodictyon_lut 1 ------------------------------------------------------ATGAAA

Figure S3: Alignment of hypD genes. The sequence stretches used for the generation of the degenerated primers are indicated.

! 14

Chlorobi-Pelodicton_lut 1 ------------------------------------------------------ATGAAA chlorobi-chlorobium_lim 1 ------------------------------------------------------ATGAAG Chlorobi-Prosthecochloris_aest 1 ------------------------------------------------------GTGAAA chlorobi-chlorobium_tep 1 ------------------------------------------------------ATGAAA delta-Syntrophus_acid 1 ------------------------------------------------------GTGAAA delta-desulfotalea_psy 1 ------------------------------------------------------ATGAAA chloroflexi-dehalococcoides_et 1 ------------------------------------------------------------ chloroflexi-dehalococcoides_CB 1 ------------------------------------------------------------ clostridia-desulfitobacterium_ 1 ---------------------------------------------------------ATG Clostr-Moorella_therm 1 ------------------------------------------------------ATGTCA Bacteroides-Robiginitalea_bif 1 ------------------------------------------------------ATGAAA epsilon-Helicobactr_acin 1 ---------------------------------------ATGAGC-----GTTAATCATC epsilon-Helicobacter_pylor 1 ---------------------------------------ATGAGC-----GTTGATCACC gamma-PhotobacteriumSKA34 1 ---------------------------------------ATGAGTTTTGAATTAAAACAG gamma-Vibrio_ang 1 ---------------------------------------ATGAGTTTTGACTTAAAACTG Gamma-Photobacterium_prof 1 ---------------------------------------ATGATGCC---ATTGAAAGAG epsilon-Wolinella_succ 1 ---------------------------------------ATGAGT-----GATCACTATC epsilon-campylobacer_con 1 -----------------------------------------------------ATGGATC Aquifex 1 ---------------------------------------ATGAGGGTTGAAGAAATTTCC gamma-Actinobacillus 1 ------------------------------------------------------ATGCAA gamma-Manheimia-succ 1 ------------------------------------------------------ATGCAG gamma-Acidobacillus_succinogen 1 ------------------------------------------------------ATGCGA Cyano-Avar 1 ------------------------------------------------------ATGAAA Cyan-Nostoc7120 1 ------------------------------------------------------ATGAAA Cyan-Nodularia_spu 1 ------------------------------------------------------ATGAAA Cyan-Lyngbya8106 1 ------------------------------------------------------ATGAAA Cano-trichodesmium_ery 1 ------------------------------------------------------ATGAAG clostridia-caldicelluosiruptor 1 ------------------------------------------------------------ clostridia-clostridium_beij 1 ------------------------------------------------------------ Methanobacteriales-Methanospha 1 ------------------------------------------------------------ Thermococcales-Pyrococcus_abys 1 ------------------------------------------------------------ Methanococcales-Methanocaldoco 1 ------------------------------------------------------------ Methanococcales-Methanococcus_ 1 ---------------------------------------------------ATGGATGCA delta-Lawsonia_intra 1 ------------------------------------------------------ATGTTA epsilon-campylobacter_jej 1 -----------------------------------------------------ATGAATT epsilon-campylobacter_ups 1 -----------------------------------------------------ATGAATT clostridia-clostridium_therm 1 ------------------------------------------------------------ clostridia-carboxidothermus_hy 1 ------------------------------------------------------------ Clostr-Halothermothrix_oren 1 ------------------------------------------------------ATGAAA Methanosarcinales-Methanosarci 1 ------------------------------------------------------ATGAAA Methanosarcinales-Methanosarci 1 ------------------------------------------------------ATGGAA Clostridia-Thermoanaerobacter_ 1 ------------------------------------------------------------ clostridia-clostridium_cell 1 -----------------------------------------------------ATGAGAA cyano-crocosphaera_wat 1 ------------------------------------------------------ATGAAA Bacteroides_Flavobacteriu_john 1 ------------------------------------------------------ATGAAA

! 15

Proteo-others-MagentococcusMC1 1 ---------------------ATGGACCAAGCAAGCATGGAACAGAAACTACTGGCCCTT beta-Nitrospira_multi 1 ---------------------------ATGACACTTACCGCTCAGGAGTGGCTAAAAAAA beta-Thiobacillus_denitri 1 ------------------------------ATGGACGAAGCACGGATCTGGCTCGAGAAA alpha-Acidiphilum 1 ---------------------ATGAGCGGCGAGGCCGAGGCCCGGCTCTGGCTCGGCCGC Thermococcales-Pyrococcus_fur 10 AAATTTGG--AGACAAAGCTGTAGCTCAAAAGATTTTAG---AAAAAATT----AAAGAG Thermococcales-Pyrococcus_hori 10 AAATTCAG--AGAAAGAGCAGTAGCACAAAGAATCATCG---AGAAGATA----AAGGAG Thermococcales-Thermococcus_ko 19 GCCTACCG--CTCGCGTGAGGTGGCCATGAAACTCGTTG---AAAAGATC----AGAGAG Archaeoglobales-Archaeoglobus 39 AGAGTTGC--AGATGAAACCG--GTAATCAGGCTTTCTG---AGAAAATA----AGGGAG Methanopyrales-Methanopyrus_ka 1 ---ATGAC--ACGTCGGGAGTTCGTGAGAAAGCTCGTCC---GTCGAATC----CATAAG Methanomicrobiales-Methanospir 1 -----------------------ATGCCCGGGAAGAATC---CGATAGCA----GAGAAA Sulfolobales-Metallosphaera_se 28 TATTACAG--GGATCCGACACTAGCTAAAGCCCTAAGAT---TGAGAATA----GAGGAA Thermoproteales-Pyrobaculum_is 28 CGGAAGAGCAAGATCACGGGCATGTTGATCCAGTATATT---AAGAAATACGCCAAAGAA Methanobacteriales-Methanother 1 -----------------------ATGAAGAATCTTTCAA---GGGAACTGG---TTTCAA Methanomicrobiales-Methanocull 1 -----------------------------ATGGCGGTAGGTAAGGAGATCCTCG---ATA Thermoproteales-Thermofilum_pe 61 TCTAACGCTAGGCTTGCCGGGGCGCTTAGGGGGTATATTGCCAGGAAGTTCGAGGAGATA delta-Anaeromyxobacter 29 TCAAGTTCCGCGATCCGGCCCGCGCCCGCGCGCTCGCCG---AGGCGCTCC----GCCAC delta-desulfovibrio_des 1 ------GTGAACTTTTCCAATGCGTTCAAAGATCCTGTGCTGTGCCGCGGGCTGCTGGAC delta-geobacter_metalli 7 TACCAGGACGAATTCCGCGACCGCCACGTGGTGCTGGGCCTTGCCGCCCGGATCAGGGAG delta-Pelobacter_propion 7 TATATGGATGAATTCCGCGACCGGGATGTGGTTCTCGGCTACGCGGCACAAATCAGGAAG delta-geobacterFRC32 7 TACGTTAAACTTTTTGGTGATAAAGAGATTGTCAATGGCTATGCCGAGCGCATCGAGGCG proteo-others-Mariprofundus_fe 7 TACGTCGATGAGTTTCGGAATGGTGATACAGCCAGCGCGCTGGCCCGACTTATCCGGGCA alpha-Stappia_agg 7 TACATCAAGGAATTTCGGGATGGAAAGCTGGCGCAAAAGCTTGCCCTGGCCATCCGCCAG beta-Methylibium_petro 7 TACATCGACGAGTTTCGCGACGGCGGCGTCGCCCAGAAGATCGCCGAGCGGCTGCGCCAG beta-Ralstonia_metall 7 TACGTGGACGAATTCCGGGACGGCGAGCTGGCCCGTACCCTGGCGGCGAAGATCGCCCGC beta-Rhodoferax_ferri 7 TACATCGACGAATTCCGTGACGGCGAGGTCGCCAAAACCTTGGCCCTCAAGATCGCCCGG beta-dechloromonas_aro 7 TACATCGACGAGTTCCGCGACGGCGACGTCGCGCAGACGCTGGCTGCCGCCATTCGCGCC beta-Azoarcus 7 TACGTGGACGAATTCCGCGACGGCGAACTCGCCCGCGGCCTTGGCGAGGCGATCCGGCGC beta-Burkholderia-viet 7 TACATCGACGAATTCCGCGACCGCGCGCTCGCCGAACGCATTTCCGCGCGCATTCAGGCG alpha-Magnetospirillum_mag 7 TACATCGACGAATTTCGCGACGGCGAGGTGGCGCAAAGCATCGCCCAGGCCATCAAGGCC alpha-Bradyrhizobium_jap 16 TATTCCAGCGAATTTCGCGACAAGACTATCGCGCAGGGACTTGCGCGAGCAATTGACGCT alpha-BradyrhizobiumBTAi1 7 TATGCCGACGAGTTTCGCGACAAGGAGCTGGCGACGGGTCTTGCGCGCGCCATCCGCGCC alpha-Rhodopseudomonas_palBisA 7 TACGTCGACGAATATCGCGACGGCAAGCTGGCGCGCAAACTCGCCGCCGCGATCGCGCAA alpha-Rhodopseudomonas_palCGA0 7 TACGTGGACGAATATCGCGACGGCAAGGTGGCGCGGCAGATCGCGGCGGCGATCGCACAG beta-Burkholderia_phy 7 TACGTCGACGATTTTCGCAATAGCGACCTCGCACGTCAGATCGCGAGGCGTATTCATGTC alpha-Rhodospirillum_rub11170 16 GACGGCGCGGCTTTTCAGGACCCCGAGCGGGTGCGCGCCCTGGCCACGGCCATCGCCCAG beta-Burkholderia_xeno 7 TACGTCGACGAATTCCGCGATCCGGACAGAGCCCGTGCGCTGACGCACGAGATTCGCCGG alpha-Sphingopyxis_alask 7 TATGTCGACGAATTCCGCGATCCGGTTCGCGCCGGTGTCCTGCTCAAGGAAATCGAGGCC gamma-Shigella_flex 7 TTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAA gamma-Shigella_sonn 7 TTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAA gamma-Shigela_dys 7 TTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAA gamma-EcoliK12 7 TTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAA gamma-Ecoli_CFT073 7 TTTGTTGATGAATATCGCGCGCCGGAACAGGTAATGCAGTTAATTGAGCATCTGCGCGAA gamma-salmonella_ent 7 TTTGTTGACGAGTACCGTGCGCCGGAACAGGTAATGCAGCTCATTGAACACCTGCGTGAA gamma-salmonella_LT2 7 TTTGTTGACGAGTACCGTGCGCCGGAACAGGTAATGCAGCTCATTGAACACCTGCGTGAA gamma-enterobacter_638 7 TACGTTGATGAATATCGCGCGCCGGAACAGGTGATGCGGCTTATCGCCCACCTTAAAACC gamma-erwinia_caro 7 CTTGTCGATGAATACCGCGATCCCGAGAAAGTGATGGCGCTTATTGCCCACCTTAACCAG gamma-Yersinia_ber 7 TACGTTGATGAGTTTCGTGATCCCGCATTAGTGAGCGCATTATTGCAGCGGATCGAACAG gamma-Yersinia_int 7 TACGTTGATGAGTTTCGTGATCCCGCATTAGTGAGCGCATTATTGCAGCGGATCGAACAG gamma-Yersinia_fred 7 TACGTTGATGAGTTTCGTGATCCAGCATTAGTGGCAGCATTGCTGGGCCGTATTGAAAAC gamma-Yersinia_moll 7 TACGTTGATGAGTTCCGCGATCCCGCCTTAGTGAGCGCCTTACTGAGCCGGATCGAACAC gamma-Serratia_protea 7 TACGTTGATGAGTTCCGCGATCCGAAGCGGGTAAAAACCCTGTTGCAGCATATTCGGCAG alpha-Rhodobacter_sphaer 7 TTCGCGTCCGAGTTCCGCGATCCCGCCCTCGCGCGGAGCCTTCTGGCGGCCATCGCCGCG alpha-Rhodobacter_spaer17025 7 TTCGCCTCGGAGTTCCGCGACCCGACGCTGGCGAAGTCGCTGATTGCCGCCATCTCGGCG alpha-Roseovarius-2601 7 TATGTCGAGGAATTCCGCGATCCCAAGGCCGCCAAGGGCGTGTTGGCGGCCATCGCCCGC alpha-Paracoccus_deni 7 TTCGCTTCGGAATTCCGCGATCCCAAGGCGGCGCGCGCGCTGCTGGCCGCGATTGCCCGC alpha-Xanhobacter_auto 7 TACGTCGACGAATTCCGCGACCCCGCCCTCGCCTCGGCGCTGGTGAAGGAGATCGGCGCC actinobacteria-corynebacterium 7 TACGTAGATGAGTTCCGCGATCCTGAAGCAGCCAAAGCACTTCTGAATCGTATCGAAAAA Actinobacteria-RhodococcusRHA1 7 TTCGTCGACGAATTCCGGGACCCGGCGGCGGCTCGCGCCCTGGTCAAATCCATC---ACC Chloroflexi-Acidobacteria 7 TTTGTCGATGAGTACCGCGACCGCGAAAAAGCTGAGCAATACGCACGCGCCATTCGAACC Chloroflexi-Solibacter_usi 7 TACCTTGACGAGTACCGCGACGACCGGATCGCGAAGAAGGTGGTGGACGAGATCCGGCGG delta-proteo_MMS1 7 TTTATCGACGAATACCGCGATCCCGCCCTGGCCCGGGAGCTGCTGGCCGGGATTGCCGCC Cyan-SynechococcusWH5701 7 GGCAGCCCCCTCCACCGTCAGCGGCAGCAGGTGCAGCGGCTGCTCGACGCGCTGCACGCC Cyan-Synechocystis6803 7 TACGTTGATGAATATCGGGATGCCCAGGCGGTGGCCCATTACCGTCAGGCGATCGCCAGG chloroflexi-chloroflexus_agg 7 TATCTTGACGAATTTCGTGACCCAGCGCTGGCCCGCCGCCTCTTCGAGCAAATCCGACGC chloroflexi-chloroflexus_aur 7 TATCTTGATGAATTTCGTGATCCTGAACTGGCCCGCCGCATGTTCGATCAGATTCGACGC Chloroflexi-Roseiflexus_cas 7 TACCTGGACGAATATCGCGACCCGGATCTGGCGCAGCGGCTTTTTGCCGAGATTCGTCGC Chloroflexi-RoseiflexusRS-1 7 TATCTCGACGAATATCGTGACCCGGAACTGGCGCAGCGCCTGTTCGCCGAAATTCGTCGT Actinobacteria-Mycobacterium_g 7 TATCTGGACGAGTTCAGCAATCCCGAACTGGCGCATCGCCTCATCGAGCAGATCGAAGCG Actinobacteria-Mycobacterium_v 7 TACCTGGACGAGTTCAGCAACCCGGAGCTGGCGGCGCGGCTCATCGAGCAGATCACGTCG Actinobacteria-FrankiaCcI3 7 TATCTGGAGGAGTTCCGGGATCCGGAGCTGGCCGGTCGGCTGCTGGAACAGATCCACGCG Actinobacteria-FrankiaACN14a 7 TACCTCGAGGAGTTCAGCGACCCCGCCCTGGCCGGTCGACTGCTGGACCAGATCCATGCG Actinobacteria-Streptomyces_av 7 TACATCGACGAATTCCAGGACCCGGAACTGGCGCGCCGGCTCCTCGACGACATCCACTCC Actinobacteria-Mycobacterium_s 7 TACCTCGACGAGTTCCGTGACCCCGTCGCGGCGCGCACCCTGGTCGACCGCATCAACCGG Chlorobi-Pelodictyon_lut 7 TATATCGATGAGTACCGCAATCCCAGGCTTGCGCGGACCCTTCTTGAGGCGATCAGGAGA

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Chlorobi-Pelodicton_lut 7 TATATCGATGAATATCGCGATCCACAGCTTGCCCGGAAGCTGCTTGACGAGATTCGCAGA chlorobi-chlorobium_lim 7 TTCATCGATGAATACCGGGATCCGCTGATCGCGCTTCGTCTTGCGGACGAGATAAAGCGC Chlorobi-Prosthecochloris_aest 7 TATATCGATGAGTACCGTAATCCGGATCTTGTTCGGCTTCTGCTCGAACAGATCGACAAC chlorobi-chlorobium_tep 7 TTCATCGACGAATACCGCGACCCCGCTCGCGCTCGCGCTTTGCTTGACCGCATCCGGCAG delta-Syntrophus_acid 7 TTTATTGACGAATACCGGGATCCCGATCTCGTTCGGCACCTTCTCGATGCCGTGGCGGCG delta-desulfotalea_psy 7 CATATTGACGAATATCGAGACGCCAAGCTCGCCAAACCACTGGTCGCCGAGCTCAGAAGG chloroflexi-dehalococcoides_et 1 -------GTGAATTTCAT--AGAGGAATTCCGCAGCAGCCAGTCAGGACG---CACC--- chloroflexi-dehalococcoides_CB 1 -------GTGAATTTCAT--AGAGGAATTCCGAAACAGCCAGTCAGGGCG---TACC--- clostridia-desulfitobacterium_ 4 CACGTGAATAATTATAATTCAAAGCAATCGGTTAAAGAAGTGGCGG--CGGGCTATC--- Clostr-Moorella_therm 7 CCCCTGGACAGGTTTTGTGATACGGAACTAAGCAGGAAACTCATGGGCCGGGTTGCCGCC Bacteroides-Robiginitalea_bif 7 TTTCTTAGCGAATACAGGGATGCCGATTTGGCAAAAAAATATTTGGCTGAAATACATTCC epsilon-Helicobactr_acin 17 TCATTTCGCC-TTTTAGAGACAAGCAAACCATTTTAGCGCTCTCTAATGAAATCAAAAAA epsilon-Helicobacter_pylor 17 TCATTTCGCC-CTTTAGAGACAAGCGAACCCTTTTAGCGCTCTCTAATGCAATCAAAAAA gamma-PhotobacteriumSKA34 22 CTATATCAAGGCTTTCGTGATGCTGATACTGTACTGAGTTTAGCCGAGCAAATAAAAGCC gamma-Vibrio_ang 22 TTATATCAAGGCTTTCGTGATGCTGATACTGTACTGAGTTTAGCCGAGCAAATAAAAGCG Gamma-Photobacterium_prof 19 CTGTATCAAGGGTTTCGTCAGCCGGATGTTGTTAAGGCTATCGCGGAAAAAATTGCTCGG epsilon-Wolinella_succ 17 TCAT-CGATTCTTTTCGAGACAAAGAGATCATCCAAAAATTTGCTAAGAAAATCGCCCAA epsilon-campylobacer_con 8 TTAT-CAATGACTTTCGCGATAAAAATTTAATCCTAGCCCTTTCAAAACTCATCCAAAAA Aquifex 22 ATCTTTGAGGCCTATAAAGATAAAGAAAGGGTTTTAAAACTCTCCGAAAAGATAAAAAAA gamma-Actinobacillus 7 TTTGTCGATGAATTTAGAGATCCCGTTCTGGCTCGTAAATTAATTACGCATTTACAGCAA gamma-Manheimia-succ 7 TTCGTGGATGAATTTCGCGATCCTAAATTGGCTAAACATTTAGTTGAACGATTAACAAAA gamma-Acidobacillus_succinogen 7 TTTGTTGACGAATTCCGCGATCCGAAACTGGCGAAAAGCCTGGTGCGCCGGCTGCAAAAA Cyano-Avar 7 TACGTCGATGAATTTCGGGAACCAGAAAAAGCTGAAGCCTTACGTCGTGAGATAGAAAAA Cyan-Nostoc7120 7 TATGTCGATGAATTTCGGGAACCAGAAAAAGCTGAAGCCTTACGCCGTGAGATAGAAAAA Cyan-Nodularia_spu 7 TACGTCAACGAATTTCGAGAACCAGAAAAAGCAGCAGCCTTACGCAACCAAATAACCCAA Cyan-Lyngbya8106 7 TATGTTGATGAATTTAGAGATCCGCAAAAATTGCAAGCCTTAATGGGAGAAATTAACCGC Cano-trichodesmium_ery 7 TACGTTAAAGAATTTCGCGACCCCGACAAAGCACAAGGTTTAGTAAGAGAAATTGCTCAA clostridia-caldicelluosiruptor 1 ------------ATGCAAACAGCTGAGCTCATTTGGGAGATAAAAAACAATATCGAAAAA clostridia-clostridium_beij 1 ------------ATGAATAAAGAAATCTTGATTAAAAATATTCTTAACGAAATTCAAAAG Methanobacteriales-Methanospha 1 ------------------ATGAAGAATTTAACTAAAGATATAATTAAAAAAATAAATGAA Thermococcales-Pyrococcus_abys 1 ------------------ATGAAGAATTTAACTAAAGATATAATTAAAAAAATAAATGAA Methanococcales-Methanocaldoco 1 ---ATGATTAATATCAATGATAGAGCCTTAATAAAAAAAGCCATAGATAAGATAAACAAA Methanococcales-Methanococcus_ 10 AAGATCATGAACATCAATAATAAAGAATTAATTAAGAAATCTATTCAGACAATCCAAAAA delta-Lawsonia_intra 7 GCTACACAAAAGCTGAGTGATCCAGTTCTATGTAAGGTTTTACTTAATAAATTAAATAAC epsilon-campylobacter_jej 8 TCATCGATGAATTTAGAGATAAAGAAAGCATTTTAGCTTTAAAAAAACTTATAGAACAAG epsilon-campylobacter_ups 8 TCATCGATGAATTTAGAGACAAAAATACCCTTTTAGCACTTAATGCTTTAATTAGAAAAA clostridia-clostridium_therm 1 ---------------------ATGAAGACGCTTGAAGCTATAAAAAAGGAGCTTGGAGAA clostridia-carboxidothermus_hy 1 ------ATGGGAATGACAAACAGCAGCATATTAAAATCTTTTCGTGAAATTATGGAAATA Clostr-Halothermothrix_oren 7 TGTGTTGATGAGTTGTCAGATAGGAAAATAATCAGCGGAATAGTCAGTAAAATTAAGGCA Methanosarcinales-Methanosarci 7 AACGAGGACTCTTCTTTAAAGCCTTCAATGCCCGAAATTGAAAAAAAGCTCCTGGAAAGA Methanosarcinales-Methanosarci 7 AACGGGAATTCACCTTCAAAGCCAGCGATTCCTGAAATTGAAAAAAAGCTTCTGGAAAGA Clostridia-Thermoanaerobacter_ 1 ---------ATGATGCAGCAGAGGGAAATAGTTGAAAAAGCCAAAAAAGCCATTGAAACA clostridia-clostridium_cell 8 ACTATATAGATGATTTCAGGGACAGTGAGGTTATAAGCAGAATCGTACAAAGCCTGCAAG cyano-crocosphaera_wat 7 TTTGTTGATGAATATCGTAATGCTGATCTAGCTCAAAAGTATGTCCAAGAGATCACACAA Bacteroides_Flavobacteriu_john 7 TACCTGTCTGAATATCGAAATCCGGAACTGGTTAAACACTACATAAGTGAAATCCATAAA

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Proteo-others-MagentococcusMC1 40 ACCCGT---TTGCAGGTAAGTGAGCCTATT------------------AAGGTGATGAAT beta-Nitrospira_multi 34 ATCCATGAGCTGCCACTCGATCGCCCTGTA------------------CGTATCATGAAC beta-Thiobacillus_denitri 31 GTCCACGCGCTCGCGTTCGACCGCAAGGTC------------------AAGATCATGAAC alpha-Acidiphilum 40 ATCCGCGCCCTGCCGCCGGGCCCGCCGGTC------------------GCCATCATGAAT Thermococcales-Pyrococcus_fur 61 GAAGCTAAAGGGATAGAAG---AGCTA---------------------CGATTTATGCAC Thermococcales-Pyrococcus_hori 61 GAAGTCAAGGGAATGGAAG---AGGTT---------------------AGATTCATGCAC Thermococcales-Thermococcus_ko 70 GAAGCGAAGACCCTCGACGGTGAAATC---------------------AGGATAATGCAC Archaeoglobales-Archaeoglobus 88 CTCTCAAAGGAGTTTGAGT---CGGTC---------------------AAAATAATGCAC Methanopyrales-Methanopyrus_ka 49 GAGGCCGAGACCCTCGATCGTGATGTG---------------------ACGATCATGCAC Methanomicrobiales-Methanospir 31 ATTCATGAGCTGACTGACCGCGATCTT---------------------ACGTTTATGCAT Sulfolobales-Metallosphaera_se 79 TTGGCGAAGAAAATCCAGGAAAACATA---------------------ATGATAATGCAC Thermoproteales-Pyrobaculum_is 85 CTCAAGAAGAGAGATGGGGACTACGTATAT------------------AAGGTCATGGAC Methanobacteriales-Methanother 32 GGATACAGGAGATATCACGTCCAGTTA----------------------AGATAATGCAT Methanomicrobiales-Methanocull 29 CGCTTCACGCTCTCGTGG-ACCGTGATATC------------------ACGCTCATGCAC Thermoproteales-Thermofilum_pe 121 AGGGCTAGGGAGGGGCGGGACTACAGGATC------------------AAGATCATGGAC delta-Anaeromyxobacter 82 AACATGGACGCCATCGGCCGCGAGGTGT---------------------CGGTGATGCAC delta-desulfovibrio_des 55 CGGCTTCGTGCCGAGACAGACGCCCCGCTG------------------CGTTTCATGGAA delta-geobacter_metalli 67 CACGTGGCCGGGCGTACGGAGCCCATG---------------------ACCTTCATGGAG delta-Pelobacter_propion 67 CTGTCTGAGCAGCGCCCGGAACCGATG---------------------GCTTTCATGGAG delta-geobacterFRC32 67 CTGACTGCCGGCCTGGAAGTGCCTCTG---------------------ACCTTGATGGAG proteo-others-Mariprofundus_fe 67 GAGGTAAAGCCCGGGAGAAACTACCGCC------------------------TGATGGAG alpha-Stappia_agg 67 GAAGCCGACCCGGGCCGGCGTTACCACC------------------------TGATGGAG beta-Methylibium_petro 67 GAGGTTCGTCCGAACCGCAGCTACAGCT------------------------TCATGGAG beta-Ralstonia_metall 67 GACGCCGACTCCGGGCGCAGCTACAGTT------------------------TCATGGAG beta-Rhodoferax_ferri 67 CTGGCGCAGCCTGAGCGCAGCTACAGCT------------------------TCATGGAA beta-dechloromonas_aro 67 GAGGCTGACCCGGCGCGCAGCTACCATT------------------------TCATGGAG beta-Azoarcus 67 GAGGCCGATCCCGGCCGCCACTACGCCT------------------------TCATGGAG beta-Burkholderia-viet 67 GAGGCGCAGCCCGGCACGCGCTATCACT------------------------TCATGGAA alpha-Magnetospirillum_mag 67 GAGGCCGATCCCGGCTCCACCTATCACA------------------------TCATGGAG alpha-Bradyrhizobium_jap 76 GAGGTCAGTTCACAACGAGCCTACCGGT------------------------TCGTGGAA alpha-BradyrhizobiumBTAi1 67 AAGGCCGATCCATCGCGCGCCTATCGCT------------------------TCATGGAG alpha-Rhodopseudomonas_palBisA 67 GCCGTCGAGCCTGCGCGCAGCTATCACT------------------------TCATGGAA alpha-Rhodopseudomonas_palCGA0 67 GCGGCCGATCCATCGCGCAGCTATCACT------------------------TCATGGAG beta-Burkholderia_phy 67 GCCGTTGAGCCGGAACGACGCTATCGCC------------------------TGATGGAA alpha-Rhodospirillum_rub11170 76 GCGGTGAGGCCGGGCAAACGCTATCGCT------------------------TCATGGAA beta-Burkholderia_xeno 67 CTAGTCGAGCGGATCGCACGCACACGCGGTGGCCTGGAGCGACCGTTGCAGATCATGGAG alpha-Sphingopyxis_alask 67 CTGACTGCACAGATCATGGCCGGGCGCGATCGCCCGG-----TCGTC----ATCATGGAA gamma-Shigella_flex 67 CGTGCTTCACATCTCTCTTACACCGCCGAACGCC---------CTCTGCGGATTATGGAA gamma-Shigella_sonn 67 CGTGCTTCACATCTCTCTTACACCGCCGAACGCC---------CTCTGCGGATTATGGAA gamma-Shigela_dys 67 CGTGCTTCACATCTCTCTTACACAGCCGAACGCC---------CTCTGCGGATTATGGAA gamma-EcoliK12 67 CGTGCTTCACATCTCTCTTACACCGCCGAACGCC---------CTCTGCGGATTATGGAA gamma-Ecoli_CFT073 67 CGTGCTTCACATCTCTCTTACACCGCCGAACGCC---------CTCTGCGGATTATGGAA gamma-salmonella_ent 67 CGCGCCGCCCTTCTTCCTTACACTGCTGAACGAC---------CGCTGCGCATTATGGAA gamma-salmonella_LT2 67 CGCGCCGCCCTTCTTCCTTACACTGCCGAACGAC---------CGCTGCGCATTATGGAA gamma-enterobacter_638 67 CGGGCAACATTACTCGATTACACCGCACAGCGTC---------CGCTGCGGATTATGGAA gamma-erwinia_caro 67 CGGGCCCCGATGCTTTCTTATACCAAACAGCGTC---------CATTGCGCATTATGGAG gamma-Yersinia_ber 67 TTATTGCCGCAGATCGCGGATCAACAGCGCTTGC---------CACTGCAAATTATGGAA gamma-Yersinia_int 67 TTATTGCCGCAGATCGCGGATCAACAGCGCTTGC---------CACTGCAAATTATGGAA gamma-Yersinia_fred 67 TTACTGCCACAGATCCCGAATCAGCAGCGTTTGC---------CGCTGCAGATCATGGAG gamma-Yersinia_moll 67 TTACTGCCGCAATTGCCGGATCAATCGCGTTTGC---------CGTTGCAGATCATGGAG gamma-Serratia_protea 67 CGAGCCGCACAGTTGCCGTTTAGCGCTGAGCGGT---------CGTTGCAAATTATGGAA alpha-Rhodobacter_sphaer 67 CGCGCCGACGAGATCGGCGCCACCCGCGCCCGCC---------CGCTGCACATCATGGAG alpha-Rhodobacter_spaer17025 67 CAGGCCGACCGGCTCGGCGCCCGCCGGGAACGGC---------CGGTGCACATCATGGAG alpha-Roseovarius-2601 67 GTCGCGGACGAGATCGGCGCCACCCGCGACGCGC---------CAGTGCGCATCATGGAG alpha-Paracoccus_deni 67 CGCGCCGAGCAGATCGGCGCCAGCCGCGAAAAGC---------CGGTGCATATCATGGAG alpha-Xanhobacter_auto 67 ATCTCGGTCCGGCTGGAACGCGGCCGCGACCGGC---------CGTTCGCCATCATGGAG actinobacteria-corynebacterium 67 GACGCGGCCCAGCTTGGCGAGATCTCGC------------------------TCATGGAG Actinobacteria-RhodococcusRHA1 64 GAACTGGCCCGAGGTGACGAGTTCAAGT------------------------TCATGGAG Chloroflexi-Acidobacteria 67 GAAGCCACGCGCCCCTGGTCCA------------------------------TCATGGAA Chloroflexi-Solibacter_usi 67 ACGGTCACGCGGCCGTGGGTGA------------------------------TTATGGAG delta-proteo_MMS1 67 ACCGTCAGCCGCCCCTGGGTGA------------------------------TCATGGAG Cyan-SynechococcusWH5701 67 GAGATCAGCAGGAGCTGGACGC------------------------------TGATGGAG Cyan-Synechocystis6803 67 GAGATAACCAAACCTTGGACGC------------------------------TGATGGAG chloroflexi-chloroflexus_agg 67 ATCACCACACGTCACTGGGCAA------------------------------TTATGGAA chloroflexi-chloroflexus_aur 67 ATCACCACCCGCCCCTGGGCGA------------------------------TTATGGAG Chloroflexi-Roseiflexus_cas 67 ATCACGACGCGCCCCTGGGCGA------------------------------TCATGGAA Chloroflexi-RoseiflexusRS-1 67 ATCACCACGCGCCCGTGGGCGA------------------------------TTATGGAA Actinobacteria-Mycobacterium_g 67 GTCGCCACCCGCCGCTGGTCGA------------------------------TCATGGAA Actinobacteria-Mycobacterium_v 67 GCCGCCACCCGCCGCTGGTCGA------------------------------TCATGGAA Actinobacteria-FrankiaCcI3 67 ACGACGACTCGCCCATGGTCGA------------------------------TCATGGAG Actinobacteria-FrankiaACN14a 67 GTGACGACCAGGCCGTGGGCGA------------------------------TCATGGAG Actinobacteria-Streptomyces_av 67 ACGGTGACCAGGCCGTGGGCCC------------------------------TGATGGAG Actinobacteria-Mycobacterium_s 67 GCCGCGACAAGAACCTGGACCA------------------------------TGATGGAA Chlorobi-Pelodictyon_lut 67 CGGGTAAGCCGACCTTGGACCA------------------------------TTATGGAG

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Chlorobi-Pelodicton_lut 67 AAAACCACCCGCCACTGGACCA------------------------------TCATGGAG chlorobi-chlorobium_lim 67 AGGGCGACGAGGCGATGGACCA------------------------------TCATGGAG Chlorobi-Prosthecochloris_aest 67 GCGGTAAGCCGGCCATGGACGA------------------------------TTATGGAA chlorobi-chlorobium_tep 67 GTGGCGCGTCATGACTGGACGA------------------------------TCATGGAA delta-Syntrophus_acid 67 CTGGCAGCCCGGGTCGGCCGTCGGATCA---------------------GCCTGATGGAG delta-desulfotalea_psy 67 TCAGTAACCAAGCCCCTGCGCA------------------------------TAATGGAA chloroflexi-dehalococcoides_et 46 CTGCTGGAATATATAAACAAACGTTCCACCAAGCCTGCCCGCT---------TTATGGAG chloroflexi-dehalococcoides_CB 46 CTGCTGGAATACATAAATAAACGTTCCACCAAGCCTGCCCGCT---------TTATGGAG clostridia-desulfitobacterium_ 59 ----TAAAAGAAATTCGGGAATTAGC--GGCAGCCCCCTATACG-------GTTATGGAG Clostr-Moorella_therm 67 CTGGCAGATGAGGTTGCGGAACGCCTTGGGCGGCCGGCAGTTT---------TTATAGAA Bacteroides-Robiginitalea_bif 67 ACGGTTACCCGGGAATGGAATG------------------------------TTATGGAA epsilon-Helicobactr_acin 76 CTCGCCTCCAAACTCACAGAAAAATTAGTC---------------------ATCATGGAA epsilon-Helicobacter_pylor 76 CTCGCTTTCAAACTTGAAAAAAAATTAGTC---------------------ATCATGGAA gamma-PhotobacteriumSKA34 82 ATTGCGCCACAATTACCAGAGCCTTTAAAA---------------------GTGATGGAA gamma-Vibrio_ang 82 ATAGCGCCACAATTACCAGAGCCTTTAAAA---------------------GTTATGGAA Gamma-Photobacterium_prof 79 CGGGCCACTGAGCTTCAACAGCCACTTTAT---------------------ATCATGGAG epsilon-Wolinella_succ 76 AATGCCTCTTTATTGCCTAGAAAAGTGAAA---------------------ATTATGGAG epsilon-campylobacer_con 67 ---------GAGAGCGTAAAACCGCTAAAC---------------------ATCATGGAA Aquifex 82 CTTTCTGAAAAGATAGGAAGAACGGTAAAC---------------------ATAATGGAG gamma-Actinobacillus 67 TTAATGCAAAAGTTGCCGCAATTCAGTAAAAAACGACCG------CTTTATTTGATGGAA gamma-Manheimia-succ 67 TTAATGAAAAATTTACCGCAGTTTTCGGCAAAAAATCCA------TTGTATTTAATGGAA gamma-Acidobacillus_succinogen 67 CTGATGGAAAAACTACCGCACTTTACGGCGGAAAACCCT------TTGTATTTAATGGAA Cyano-Avar 67 CTCAGCCAGCAATT---AGA---------TAAACATA---------TCAAGATTATGGAA Cyan-Nostoc7120 67 CTCAGCCAGCAATT---AGA---------TAAACATA---------TCAAAATTATGGAA Cyan-Nodularia_spu 67 CTAAGCAACCAACT---AAA---------CAAACCCC---------TTAAACTGATGGAA Cyan-Lyngbya8106 67 TTAACGTTAATCCT---AGAACGGACACCCGAACACCCG------TTAAAAATCATGGAA Cano-trichodesmium_ery 67 CTCAGCCAAAAGTT---AGGATACACCACGGAACACCCA------CTAAAAATAATGGAA clostridia-caldicelluosiruptor 49 ATT------GGAC------G------------GAGTC---------TAAAAATCATTGAA clostridia-clostridium_beij 49 TCT------CCAGT---CAA------------AAAGT---------TTAATGTTATGGAA Methanobacteriales-Methanospha 43 ATA------AAAC------A------------GCCTA---------TTAAAATTATGCAT Thermococcales-Pyrococcus_abys 43 ATA------AAAC------A------------GCCTA---------TTAAAATTATGCAT Methanococcales-Methanocaldoco 58 CTTGCTGAGAAGAT---AGA------------TAAAT---------TAAAGATTATGCAC Methanococcales-Methanococcus_ 70 CTCTCTGAAAAACT---GGA------------CGAAG---------TAAAAATAATGCAC delta-Lawsonia_intra 67 CTT------TTAGA---TCG------------TCCTT---------TAAGATTTATGGAG epsilon-campylobacter_jej 68 AACTT----AAAA------A------------TCCTA---------TCAATATCATGGAA epsilon-campylobacter_ups 68 ATATT----AAAG------A------------GTCTA---------TAAATATAATGGAA clostridia-clostridium_therm 40 TAC------GAGGG---CAA------------AAACA---------TTAAAATAATGGAA clostridia-carboxidothermus_hy 55 GTTCAGAAAAGCGA---CTT------------AACCA---------TTAATATTATGGAA Clostr-Halothermothrix_oren 67 ATTAA---ACTATC---GGG------------AAAAG---------TAAAAATTATGGAA Methanosarcinales-Methanosarci 67 ATTCAGAAATCCGG---GAC------------CTCGC---------TCCGCATAATGCAT Methanosarcinales-Methanosarci 67 ATCAGAGACTTTGA---TAA------------ACCGC---------TTCGCATAATGCAT Clostridia-Thermoanaerobacter_ 52 CTAAAT---CCGGG---TAT------------CCCTT---------TTAACATAATGGAA clostridia-clostridium_cell 68 GTTAC----TCCGG---GCC------------TAAGT---------TGAAAATAATGGAA cyano-crocosphaera_wat 67 ACA------ATTAC---TAG------------ACCTT---------GGACAATCATGGAA Bacteroides_Flavobacteriu_john 67 ATA------ACCAC---AAA------------ACCAT---------GGAATATCATGGAA

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Proteo-others-MagentococcusMC1 79 TTGTGTGGAGGATTTGCCCGGTTTTGGGCCGAGAGCGATTGGCATCAATCCCTTA----- beta-Nitrospira_multi 76 GTATGCGGAGGCCATGAGCG-TTCGATTACCATGGCAGGAATCCGAAGCGCCCTG----- beta-Thiobacillus_denitri 73 GTATGCGGCGGGCACGAGCGCTCGATCACCCATGCCGGACTGCG-CGGCGCGCTG----- alpha-Acidiphilum 82 GTCTGCGGCGGGCATGAGCGCAGCCTCAGCATGCTCGGCCTGCG-CGCCCTCCTG----- Thermococcales-Pyrococcus_fur 97 GTTTGTGGGACTCATGAGGACACAGTAACTAGGAGTGGAATCAGATCACTTCTTC----- Thermococcales-Pyrococcus_hori 97 GTTTGTGGAACGCATGAGGATACCGTAACGAGGAGTGGAATAAGATCCCTACTAC----- Thermococcales-Thermococcus_ko 109 GTCTGCGGAACCCATGAGGACACGGTAACGAGGCACGGAATCCGCTCGCTTCTTC----- Archaeoglobales-Archaeoglobus 124 CTCTGCGGAACTCATGAAGATACCATCACAAAGTACAATCTCCGCTCCCTCCTCC----- Methanopyrales-Methanopyrus_ka 88 GTGTGTGGATCCCATGAACGGACTATAGTCGAACACGGTCTCAGATCGTTGCTTC----- Methanomicrobiales-Methanospir 70 GTATGCGGAACGCATGAGGCCGCTATTGCACGTCATGGAATCCGAAGCCTTCTTC----- Sulfolobales-Metallosphaera_se 118 GTGTGTGGAAGCCATGAGTGGACAATAACCCACTATGGTATAAGATCTCTCCTAC----- Thermoproteales-Pyrobaculum_is 127 TTCTGCGGCACCCACGAGTGGACTATTGTCCACTTCGGCCTTAGGAGCGTCCTAGAGATG Methanobacteriales-Methanother 70 GTATGCGGGTCACATGAGCACACAATAATGCAGCATGGTGTAAGGTCCCTCCTTC----- Methanomicrobiales-Methanocull 70 ATCTGCGGCACCCACGAGGCGGCGATCGCGCGCGCCGGGCTCAGGAGCGTCCTTC----- Thermoproteales-Thermofilum_pe 163 TTCTGCGGGACTCACGAGTGGACTATCGTGCACTTCGGGCTTAGGAGCCTCATGC----- delta-Anaeromyxobacter 121 GTGTGCGGCAGCCACGAGCAGGCCATCGCCCGCTTCGGCCTGCGCGCGGTGCTCC----- delta-desulfovibrio_des 97 GTGTGCGGCACACATACCGTGGCCATATTCCAGAGCGGTCTGCGCCCGCTGCTGC----- delta-geobacter_metalli 106 GTCTGCGGCACTCATACCATGTCGATCTATCAGTACGGGCTCCGCACCCTCCTCC----- delta-Pelobacter_propion 106 GTCTGCGGCACCCACACCATGTCCATCTACCAGTACGGCATCCGCAGCCTGCTTC----- delta-geobacterFRC32 106 GTCTGCGGTACCCATACTATGGCCATCAGCCGGTTCGGCATCAGGTCTCTTCTCC----- proteo-others-Mariprofundus_fe 103 TTTTGCGGCGGCCATACCCATGCGATCTTCCGCTTCGGCGTGAATGACCTGATGC----- alpha-Stappia_agg 103 TTTTGCGGCGGACACACCCACGCGATCTTCCGATACGGGGTGCAGGATCTCGTTC----- beta-Methylibium_petro 103 TTCTGCGGCGGCCACACCCACGCCATCTCGCGTTATGGCGTCAGCGAGCTGCTGC----- beta-Ralstonia_metall 103 TTCTGCGGCGGCCACACCCACGCCATCTCCCGCTACGGTGTCAGCGATCTGTTGC----- beta-Rhodoferax_ferri 103 TTTTGTGGCGGCCACACCCACGCCATTTCCCGCTACGGCCTGTCCGACTTGCTGC----- beta-dechloromonas_aro 103 TTCTGCGGCGGCCACACCCACGCCATTTCTCGTTACGGCGTGTCCGACCTGTTGC----- beta-Azoarcus 103 TTCTGCGGCGGCCACACCCACGCCATCTCGCGCTACGGCGTGATCGACCTGCTGC----- beta-Burkholderia-viet 103 TTCTGCGGCGGTCACACGCATGCGATTTCCCGCTACGGCGTGACCGACCTGCTGC----- alpha-Magnetospirillum_mag 103 TTCTGCGGCGGCCACACCCATGCCATCTCGCGCTATGGGCTGGAGGATTTGCTGC----- alpha-Bradyrhizobium_jap 112 TTTTGCGGTGGACACACTCATGCAATCTCCCGCTACGGTCTTGAGGATCTGCTGC----- alpha-BradyrhizobiumBTAi1 103 TTCTGCGGCGGCCATACGCATGCGATCGCACGCTATGGTCTGGAGGACATGCTGC----- alpha-Rhodopseudomonas_palBisA 103 TTCTGCGGCGGCCATACCCACGCGATCTCGCGTTATGGTCTTGAAGACCTGCTGC----- alpha-Rhodopseudomonas_palCGA0 103 TTCTGCGGCGGCCACACCCACGCGATCTCGCGCTATGGCCTGGAAGACCTGCTGC----- beta-Burkholderia_phy 103 TTCTGTGGCGGCCACACGCACGCTATCGCGCGATATGGCTTAACGGCATTGTTGC----- alpha-Rhodospirillum_rub11170 112 TTCTGCGGCGGCCATACCCACGCCATCGGCCGCTATGGGCTGGCCGGGCTGCTGC----- beta-Burkholderia_xeno 127 GTTTGCGGCGGTCATACGCATTCGATCTTTCGGTACGGGATTCACCAGATGCTGC----- alpha-Sphingopyxis_alask 118 GTGTGCGGCGGCCACACGCATTCGATCTTCCGATACGGCATCGAGAAGATGCTTC----- gamma-Shigella_flex 118 GTGTGTGGCGGTCATACCCACGCCATTTTTAAATTCGGCCTCGACCAGTTACTGC----- gamma-Shigella_sonn 118 GTGTGCGGTGGTCATACCCACGCCATTTTTAAATTCGGCCTCGACCAGTTACTGC----- gamma-Shigela_dys 118 GTGTGTGGCGGTCATACTCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGC----- gamma-EcoliK12 118 GTGTGTGGCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGC----- gamma-Ecoli_CFT073 118 GTGTGTGGCGGTCATACCCACGCCATTTTTAAATTCGGCCTCGACCAGTTGCTAC----- gamma-salmonella_ent 118 GTCTGCGGGGGTCATACCCACGCGATTTTTAAATTTGGTCTCGATCAGTTACTGC----- gamma-salmonella_LT2 118 GTCTGCGGGGGTCATACCCACGCGATTTTTAAATTTGGTCTCGATCAGTTACTGC----- gamma-enterobacter_638 118 GTGTGTGGCGGGCACACGCATGCCATCTTCAAATTTGGCCTTGACCAGCTGCTGC----- gamma-erwinia_caro 118 GTCTGCGGAGGCCATACGCACGCGATATACCGCTTTGGTATTGATCAACTGTTGC----- gamma-Yersinia_ber 118 GTCTGCGGTGGGCACACTCATGCGATCTTCAAGTTCGGTCTGGATCAACTGCTGC----- gamma-Yersinia_int 118 GTCTGCGGTGGGCACACTCATGCGATCTTCAAGTTCGGTCTGGATCAACTGCTGC----- gamma-Yersinia_fred 118 GTGTGCGGTGGTCATACTCATGCGATCTTTAAATTTGGTCTTGATCAACTGCTAC----- gamma-Yersinia_moll 118 GTGTGCGGCGGTCATACCCACGCCATCTTTAAGTTTGGCCTCGATCAACTGCTGC----- gamma-Serratia_protea 118 GTATGCGGTGGTCATACCCATGCCATTTTCCGCTTTGGCCTGGATAAACTGCTGC----- alpha-Rhodobacter_sphaer 118 ATCTGCGGCGGCCACACCCACGCGATCTTCCGCTACGGGCTCGACCGGCTGGTGC----- alpha-Rhodobacter_spaer17025 118 ATCTGCGGCGGCCACACCCATGCGATCTTCCGCTACGGCCTCGACAAGCTGGTGC----- alpha-Roseovarius-2601 118 ATCTGCGGCGGGCACACCCACGCGATCTTCCGCTACGGGCTCGACAAGCTGGTGC----- alpha-Paracoccus_deni 118 ATCTGCGGCGGCCACACCCATTCGATCTTTCGCTATGGTCTGGACAAACTGGTGC----- alpha-Xanhobacter_auto 118 GTGTGCGGCGGGCATACCCACGCCATCTTCCGCTATGGGCTGGAATGCCTGCTGC----- actinobacteria-corynebacterium 103 ATCTGTGGTGGTCACACGCACACGATCTATCGCTACGGCTTGGAAAATCTCCTCC----- Actinobacteria-RhodococcusRHA1 100 GTGTGCGGCGGGCACACTCACACCATCTACCGTCACGGCATCGAGCATCTCCTGC----- Chloroflexi-Acidobacteria 97 GTCTGCGGCGGTCAGACCCACACCATCGTCAAGTACGCGATTGACGAAATCCTGC----- Chloroflexi-Solibacter_usi 97 GTCTGCGGCGGGCAGACGCATTCGATCGTGAAATACGGGATCGACTACCTGCTGC----- delta-proteo_MMS1 97 ATCTGCGGCGGTCATACCCATGCCATTATCCGCCATGGGATCGATCAACTGCTGC----- Cyan-SynechococcusWH5701 97 GTCTGCGGCGGCCAGACCCACGCGATCCTGCGCCACGGCCTCGATCAGCTGATCC----- Cyan-Synechocystis6803 97 ATTTGCGGCGGCCAGACCCACAGCATTGTCAAATATGGCTTGGATGCTTTGTTGC----- chloroflexi-chloroflexus_agg 97 GTCTGCGGCGGCCAGACCCATTCGATCATTCGCAACGGAATCGATCAGTTACTGC----- chloroflexi-chloroflexus_aur 97 GTTTGCGGCGGTCAAACGCACTCAATCATTCGCAACGGCATCGACCAGCTCTTGC----- Chloroflexi-Roseiflexus_cas 97 GTGTGCGGCGGGCAGACGCATTCGATTATCCGCAACGGAATCGATCAACTGTTGC----- Chloroflexi-RoseiflexusRS-1 97 GTGTGCGGCGGGCAGACGCATTCGATCATTCGCAATGGCATCGATCAGCTTCTGC----- Actinobacteria-Mycobacterium_g 97 GTGTGTGGCGGACAGACACATTCGATCATCCGGCACGGCATCGACCAGCTGCTGC----- Actinobacteria-Mycobacterium_v 97 GTCTGCGGCGGCCAAACCCATTCCATCATCCGGCACGGCATCGACCAGCTGCTTC----- Actinobacteria-FrankiaCcI3 97 GTCTGCGGTGGGCAGACTCACTCGCTCATCCGGCACGGTATCGATCAGCTTCTGC----- Actinobacteria-FrankiaACN14a 97 GTCTGCGGCGGGCAGACCCATTCGCTCATCCGGCACGGGATCGACCAGTTGCTGC----- Actinobacteria-Streptomyces_av 97 GTCTGCGGAGGTCAGACGCACACCATCATCCGGCACGGCATCGACCAACTCCTCC----- Actinobacteria-Mycobacterium_s 97 GTCTGTGGTGGCCAGACACATTCGATCATCCGCAACGGCATCGACCAGCTCCTGG----- Chlorobi-Pelodictyon_lut 97 ATCTGCGGAGGTCAGACCCACTCGATCATCCGCAACGGGATAGACCAGCTGCTGC-----

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Chlorobi-Pelodicton_lut 97 ATCTGCGGTGGACAAACCCATTCGATTATCCGCAACGGCATCGATCAACTGCTGC----- chlorobi-chlorobium_lim 97 ATCTGCGGAGGGCAGACCCATTCGATCATGCGAAACGGGATCGATCAACTGCTTG----- Chlorobi-Prosthecochloris_aest 97 ATCTGCGGAGGGCAGACCCATGCAATTGTACGGCACGGGCTTGATCAGCTTCTTC----- chlorobi-chlorobium_tep 97 ATCTGCGGCGGGCAGACGCATTCGATTTTGCGCAACGGCATCGACCAGCTTTTGC----- delta-Syntrophus_acid 106 ATCTGCGGCACTCACACGCACGCCATCGGGCGCTTTGGAATCCGCAGGCTCCTTC----- delta-desulfotalea_psy 97 GTCTGTGGCTCCCACACCATGTCAATTTTTCGCAACGGTATCCGTTCAATCCTAC----- chloroflexi-dehalococcoides_et 97 TTTTGCGGCGGGCATACCGTAGCCATTTTCCGCTACGGCATCCGCCAGCTGCTGC----- chloroflexi-dehalococcoides_CB 97 TTTTGCGGAGGGCATACCGTAGCCATTTTCCGTTACGGCATCCGCCAGCTGCTGC----- clostridia-desulfitobacterium_ 106 GTATGTGGAACTCATACTGTGGCTATTGCCAAGAATGCCTTGCGGGATTTGCTGC----- Clostr-Moorella_therm 118 GTATGCGGTACACACACAGTGGCTATTTCCCGGCTGGGATTGCGGAGCTTGCTGG----- Bacteroides-Robiginitalea_bif 97 GTATGCGGCGGGCAGACCCATAGTCTGGTCAAAAACGGAATTATGGATCTGTTAC----- epsilon-Helicobactr_acin 115 GTGTGCGGAGGGCATACGCATTCTATAATGAAATACGGGCTTTTGGATCTAATGC----- epsilon-Helicobacter_pylor 115 GTGTGCGGAGGGCATACGCATTCTATCATGAAATACGGGCTTTTAGATTTGATGC----- gamma-PhotobacteriumSKA34 121 GTGTGTGGCGGCCATACACATACCATCATGAAGTATGGTTTAAAGCAGTTACTGC----- gamma-Vibrio_ang 121 GTGTGTGGCGGCCATACTCATACCATCATGAAGTATGGTTTAAAGCAGTTACTGC----- Gamma-Photobacterium_prof 118 GTGTGTGGAGGGCATACTCACACCATCATGAAGTATGGTTTAAAACAGCTATTGC----- epsilon-Wolinella_succ 115 GTTTGCGGGGGGCACACCCACACCATCATGAAATATGGACTGCTTCAGCTTATGC----- epsilon-campylobacer_con 97 ATTTGCGGAGGCCACACGCATAGCATTATGAAATTCGCACTGCCAAGTTTGGTTG----- Aquifex 121 TTTTGCGGAGGGCACACCCACGCCATAATGAAGTACGGCATAGACCAGCTCCTAA----- gamma-Actinobacillus 121 GTATGCGGCGGTCATACCCATACGATTTTTAAATTCGGTTTAGATCGTATTTTGC----- gamma-Manheimia-succ 121 GTCTGCGGCGGGCATACTCATAGTATTTTTAAATTCGGTTTAGATCGGTTATTGC----- gamma-Acidobacillus_succinogen 121 GTGTGCGGAGGGCATACCCATACCATCTTCAAATTCGGCTTGGATCGTTTGTTGC----- Cyano-Avar 106 GTATGCGGCGGGCATACTCACTCTATTTTTAAATACGGTATTGAAGAAATTTTAC----- Cyan-Nostoc7120 106 GTATGCGGCGGGCATACTCACTCTATTTTTAAATACGGTATTGAAGAAATCTTAC----- Cyan-Nodularia_spu 106 GTATGCGGTGGACATACCCACTCCATATTTAAATACGGTATAGAAGACATCCTAC----- Cyan-Lyngbya8106 118 GTTTGCGGAGGTCACACCCATTCTATCTTTAAATATGGCATTGAAGAACTTCTTC----- Cano-trichodesmium_ery 118 GTTTGTGGGGGTCACACTCACTCTATTTTTAAATATGGCATTCAAGAAGCTTTAC----- clostridia-caldicelluosiruptor 76 GTGTGTGGAACGCACACAGTTTCAATCTACAAAAATGGATTTCCTGCCGTGTTTG----- clostridia-clostridium_beij 79 GTTTGTGGAACTCATACTCAGGCACTTTCAAAACTTGGATTTAGACAATTGGTTA----- Methanobacteriales-Methanospha 70 GTATGTGGTTCACATGAACATACAATTATGTACAATGGTATACGTTCGATGTTAC----- Thermococcales-Pyrococcus_abys 70 GTATGTGGTTCACATGAACATACAATTATGTACAATGGTATACGTTCGATGTTAC----- Methanococcales-Methanocaldoco 94 GTCTGTGGAAGTCATGAGCACACAATCTGTAAGTATGGGATTAGGGATGTTCTGC----- Methanococcales-Methanococcus_ 106 GTTTGTGGTTCACATGAACATACAATCTGCAAATATGGAATAAGAGACGTTTTAC----- delta-Lawsonia_intra 97 GTTTGTGGAACACACACAGTATCTATTTTTAAAAGTGGTATAACAACCCTTCTCC----- epsilon-campylobacter_jej 97 ATTTGCGGTGGGCATACGCATAGTATTATGAAATATGCCCTGCCTTCTATTTTGC----- epsilon-campylobacter_ups 97 ATTTGCGGAGGGCATACGCATAGTATTATGAAATATGCTCTATGCGATTTACTTC----- clostridia-clostridium_therm 70 GTCTGCGGCACCCACACTTCCAGCATTTTTAAGAACGGGATCCGAAGCCTTATCT----- clostridia-carboxidothermus_hy 91 GTTTGTGGCACGCACACGATGGCGTTGTTCGAGCATGGGATTCGGAGTTTACTTC----- Clostr-Halothermothrix_oren 100 GTATGTGGGACTCATACTATGTCAATTTTTAAAAACGGGATTAAAAGTTTATTAC----- Methanosarcinales-Methanosarci 103 GTCTGCGGGACGCATGAAAGGACCATAGCAAAATACGGGCTAAGAAGTGTACTTC----- Methanosarcinales-Methanosarci 103 GTCTGCGGAACTCACGAGCGGACTATAGCAAAATATGGGTTAAGGAGTATCCTTC----- Clostridia-Thermoanaerobacter_ 85 GTGTGTGGATCTCACACGATGGCAATTTCAAAATTTGGCTTAAGGCAGGTTCTCC----- clostridia-clostridium_cell 100 GTGTGCGGAACCCATACAATGGCTATATCCAGATATGGACTCCGTTCACTTCTCC----- cyano-crocosphaera_wat 97 ATTTGTGGAGGACAAACCCATTCTATTGTTAAATATGGGATAGATCGCCTATTAC----- Bacteroides_Flavobacteriu_john 97 ATTTGCGGTGGTCAGACGCATTCATTAGTCAAAAACGGTCTGCTGGATTTATTGC-----

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Proteo-others-MagentococcusMC1 134 ------GCCGAGTG-GTGCATATCATGCCAGGGCCGGGCTGTCCGGTATGTGTCTGTCC- beta-Nitrospira_multi 130 ------CCGGAATGTGTCGAACTCATCGCCGGGCCAGGGTGCCCGGTCTGTGTCTGCCC- beta-Thiobacillus_denitri 127 ------CCGGACTGGCTCGAGCTCGTGCCCGGACCCGGCTGCCCGGTCTGCGTGTGCCC- alpha-Acidiphilum 136 ------CCGGAGGGTGTGCGCATCATCCCCGGCCCCGGCTGCCCGGTCTGCGTCTGCCC- Thermococcales-Pyrococcus_fur 152 -------CAGAAAATGTAAAAATCATGAGTGGCCCAGGATGTCCCGTCTGTATAACCCC- Thermococcales-Pyrococcus_hori 152 -------CAAAGAACGTTAAGATAATGAGCGGCCCAGGATGTCCGGTATGTATAACTCC- Thermococcales-Thermococcus_ko 164 -------CGGAGAACGTTAAGGTCGTCAGCGGGCCGGGCTGTCCGGTCTGCATAACACC- Archaeoglobales-Archaeoglobus 179 -------CGGAAAACATCTCCCTGCTGAGCGGTCCCGGCTGTCCAGTTTGCATAACTCC- Methanopyrales-Methanopyrus_ka 143 -------CCGAGAACGTCCGTTTAGTCTGCGGTCCAGGGTGCCCCGTGTGTGTGACCAC- Methanomicrobiales-Methanospir 125 -------CAAAGAATCTGAAGATTGTGATGGGACCCGGATGTCCGGTCTGTATCACTCC- Sulfolobales-Metallosphaera_se 173 -------CTGAAAACGTGGAGGTTAGGGCAGGCCCTGGTTGTCCTGTCTGCATAACGCC- Thermoproteales-Pyrobaculum_is 187 GCTGGGGTGGACAACGTAGAGCTTGTGGCTGGGCCAGGATGCCCCGTCTGCGTCACCCCA Methanobacteriales-Methanother 125 -------CTGAGGAGGTGGAGGTGGTCGCCGGGCCTGGCTGTCCGGTCTGCTGTGTCCC- Methanomicrobiales-Methanocull 125 -------CCCCGGGCCTCAAGATCGTGATGGGACCGGGCTGCCCGGTCTGCATCACGCC- Thermoproteales-Thermofilum_pe 218 -------CGGAGGGGGTCGAGCTGGTGGCTGGGCCCGGGTGCCCTGTCTGCGTCACTCCT delta-Anaeromyxobacter 176 -------CGCCCGGCCTGGAGCTCATCATGGGCCCGGGCTGCCCGGTGTGCGTCACCGA- delta-desulfovibrio_des 152 -------CGGAACAGGTCGTCCATGTGTCCGGCCCCGGGTGTCCGGTCTGTGTGACCCA- delta-geobacter_metalli 161 -------CTCCCCAGGTGCGGCTCATCTCCGGACCCGGCTGCCCGGTCTGCGTCACCCC- delta-Pelobacter_propion 161 -------CGGAAGGGGTGCGGCTCATCTCGGGGCCCGGCTGTCCGGTCTGCGTCACCCC- delta-geobacterFRC32 161 -------CGCCGAAGGTGAGGCTTATTTCCGGCCCCGGCTGCCCTGTTTGCGTCACTCC- proteo-others-Mariprofundus_fe 158 -------CTGAAAATGTCCGTTTCATTCACGGCCCCGGCTGCCCTGTTTGTGTACTGCC- alpha-Stappia_agg 158 -------CGCAAAATGTCGAGTATGTGCATGGGCCGGGCTGTCCGGTCTGCGTCTTGCC- beta-Methylibium_petro 158 -------CGGCCAATGTGCGCATGGTGCACGGTCCAGGCTGCCCGGTCTGCGTGCTGCC- beta-Ralstonia_metall 158 -------CCGCCAACGTGCGCATGATCCACGGCCCCGGCTGCCCGGTGTGCGTGCTGCC- beta-Rhodoferax_ferri 158 -------CGCCCAACGTGCGCATGGTGCACGGCCCCGGCTGCCCGGTGTGTGTGCTGCC- beta-dechloromonas_aro 158 -------CGGCCAACGTCAAGATGATTCACGGTCCAGGCTGCCCGGTCTGCGTGCTGCC- beta-Azoarcus 158 -------CGAAGAACGTGCGCATGGTGCACGGCCCCGGCTGCCCGGTGTGCGTGCTGCC- beta-Burkholderia-viet 158 -------CCGACAACGTGCGGATGATCCACGGCCCCGGCTGCCCCGTCTGCGTGCTGCC- alpha-Magnetospirillum_mag 158 -------CCGCCAATGTGCGGATGATCCACGGGCCGGGCTGCCCGGTCTGCGTCCTGCC- alpha-Bradyrhizobium_jap 167 -------CCGCGAACGTTCGGATGATCCACGGGCCCGGTTGTCCCGTCTGCGTTCTGCC- alpha-BradyrhizobiumBTAi1 158 -------CGGACAATGTCCGCATGATTCACGGTCCGGGCTGTCCTGTCTGCGTGCTGCC- alpha-Rhodopseudomonas_palBisA 158 -------CGGCCAATGTGCGGATGGTGCACGGGCCCGGTTGCCCGGTCTGCGTGCTGCC- alpha-Rhodopseudomonas_palCGA0 158 -------CGCCGAATGTTCGGATGGTCCACGGCCCCGGCTGTCCGGTCTGCGTGCTGCC- beta-Burkholderia_phy 158 -------CGCCCTGCGTCGAGTTGATCCACGGCCCCGGCTGTCCGGTGTGTGTCTTGCC- alpha-Rhodospirillum_rub11170 167 -------CCGAAGCCGTCACCCTGATCCATGGGCCGGGCTGCCCGGTCTGCGTGCTGCC- beta-Burkholderia_xeno 182 -------CGCCGGAGGTCGAGTTCGTTCATGGTCCCGGCTGCCCCGTCTGCGTGCTGCC- alpha-Sphingopyxis_alask 173 -------CGCCGGAAATCGAGTTTGCCCATGGGCCGGGCTGTCCGGTTTGCGTGTTGCC- gamma-Shigella_flex 173 -------CTGAAAACGTTGAGTTTATCCACGGTCCGGGTTGCCCGGTGTGCGTACTGCC- gamma-Shigella_sonn 173 -------CTGAAAACGTTGAGTTTATCCACGGTCCGGGTTGCCCGGTGTGCGTACTGCC- gamma-Shigela_dys 173 -------CGGAAAACGTTGAGTTTATCCACGGTCCGGGTTGCCCGGTGTGCGTACTGCC- gamma-EcoliK12 173 -------CGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCC- gamma-Ecoli_CFT073 173 -------CGGAAAACGTTGAGTTTATCCACGGTCCGGGTTGCCCGGTGTGCGTACTGCC- gamma-salmonella_ent 173 -------CCGAAAACGTGGAGTTTATTCACGGCCCCGGTTGTCCGGTCTGCGTGCTGCC- gamma-salmonella_LT2 173 -------CCGAAAACGTGGAGTTTATTCACGGCCCCGGTTGTCCGGTCTGCGTGCTGCC- gamma-enterobacter_638 173 -------CGGAAAACATCGAGTTTATCCACGGTCCGGGCTGTCCGGTCTGCGTGCTGCC- gamma-erwinia_caro 173 -------CGGAAAATATTGAATTTATTCACGGCCCAGGCTGCCCGGTGTGCGTCCTGCC- gamma-Yersinia_ber 173 -------CTGATGGGTTGGAGTTTGTCCATGGACCGGGCTGCCCAGTGTGTGTATTGCC- gamma-Yersinia_int 173 -------CTGATGGGTTGGAGTTTGTCCATGGACCGGGCTGCCCAGTGTGTGTATTGCC- gamma-Yersinia_fred 173 -------CTGATGGGCTGGAGTTTGTTCATGGGCCGGGCTGCCCGGTCTGTGTACTGCC- gamma-Yersinia_moll 173 -------CGGACGGGCTGGAGTTTGTCCACGGGCCGGGTTGCCCGGTCTGTGTGTTGCC- gamma-Serratia_protea 173 -------CGCCACAGATTGAATTTATTCATGGACCGGGCTGCCCGGTTTGTGTGTTGCC- alpha-Rhodobacter_sphaer 173 -------ATGAGGGGATCGAATTCATCCACGGCCCCGGCTGCCCGGTCTGCGTCCTGCC- alpha-Rhodobacter_spaer17025 173 -------ACGAGGGGATCGAGTTCATCCACGGCCCCGGCTGCCCGGTCTGCGTCCTGCC- alpha-Roseovarius-2601 173 -------ATGAGGGCATCGAGTTCATCCACGGCCCGGGCTGCCCGGTCTGCGTTCTGCC- alpha-Paracoccus_deni 173 -------ATGAGGGCGTGGAATTCATCCACGGCCCCGGCTGTCCGGTTTGCGTTCTGCC- alpha-Xanhobacter_auto 173 -------CGGACCTCGTGGAATTCGTCCATGGTCCGGGCTGCCCGGTGTGCGTGCTGCC- actinobacteria-corynebacterium 158 -------CCGACAACATTGACCTCGTCCATGGCCCAGGTTGTCCTGTGTGCGTCATCCC- Actinobacteria-RhodococcusRHA1 155 -------CGGAATCGGTCGAACTGGTGCACGGACCGGGATGTCCGGTGTGCGTCATCCC- Chloroflexi-Acidobacteria 152 -------GCGACAAGATCACGCTCCTCCATGGTCCCGGCTGCCCAGTGTGCGTCACGCC- Chloroflexi-Solibacter_usi 152 -------CGCCGGAGATCGAGCTGGTGCACGGGCCCGGCTGCCCGGTTTGCGTAACGCC- delta-proteo_MMS1 152 -------CGGCGGAGATCGAACTGGTGCACGGGCCGGGCTGCCCGGTCTGCGTCACCCC- Cyan-SynechococcusWH5701 152 -------CCCCGCAGCTGGAGCTGATCCATGGGCCCGGCTGTCCGGTCTGCGTCACTGC- Cyan-Synechocystis6803 152 -------CGAAGAATTTGACTCTGATCCATGGTCCCGGCTGTCCTGTGTGCGTCACTCC- chloroflexi-chloroflexus_agg 152 -------CACCCGAGATCGAGCTTATCCACGGCCCCGGCTGCCCAGTCTGCGTTACTCC- chloroflexi-chloroflexus_aur 152 -------CGCCAGAGATTGAACTGATCCACGGCCCCGGCTGCCCGGTCTGCGTCACACC- Chloroflexi-Roseiflexus_cas 152 -------CAGAAGCGATTGAGCTGATCCACGGTCCTGGCTGCCCGGTGTGCGTGACGCC- Chloroflexi-RoseiflexusRS-1 152 -------CTGAAGAGATCGAGTTGATCCACGGTCCTGGCTGCCCGGTGTGCGTCACGCC- Actinobacteria-Mycobacterium_g 152 -------CCGATACCGTCGAGATGATCCACGGACCCGGCTGCCCGGTCTGCGTCACGCC- Actinobacteria-Mycobacterium_v 152 -------CCGACACCGTCGAGATGATCCACGGGCCGGGCTGCCCGGTCTGTGTGACACC- Actinobacteria-FrankiaCcI3 152 -------CCGAGGGCATCGAGATGATCCACGGCCCCGGCTGCCCGGTGTGCGTGACCCC- Actinobacteria-FrankiaACN14a 152 -------CCGCGGGCGTCGAGATGATCCACGGCCCCGGCTGCCCCGTCTGCGTCACCCC- Actinobacteria-Streptomyces_av 152 -------CGGAACAGGTCGAGTTGATCCACGGCCCGGGCTGTCCCGTGTGCGTGACCCC- Actinobacteria-Mycobacterium_s 152 -------AGGGTTCCGTCGAGTTCGTGCACGGCCCCGGTTGCCCCGTGTGCGTCACGCC- Chlorobi-Pelodictyon_lut 152 -------CGGATACCATAGAGCTGGTACATGGACCGGGATGCCCGGTCTGCGTCACGCC- _____________________ Position of primer hypD-for1 and hypD-for2

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Chlorobi-Pelodicton_lut 152 -------CAGATACCATCGAACTGGTGCATGGCCCCGGTTGCCCGGTCTGTGTAACACC- chlorobi-chlorobium_lim 152 -------GCGATACCATCGAGCTGGTTCACGGTCCAGGATGCCCGGTTTGCGTCACGCC- Chlorobi-Prosthecochloris_aest 152 -------CTTCATCGATTCGCCTTGTTCATGGTCCAGGGTGCCCGGTTTGTGTTACTCC- chlorobi-chlorobium_tep 152 -------CGCCGAACGTGCAGCTTGTGCATGGGCCGGGGTGTCCGGTGTGCGTGACGCC- delta-Syntrophus_acid 161 -------CCGAAAACATCCGCCTCATCTCCGGCCCCGGATGCCCCGTCTGCGTCACCTC- delta-desulfotalea_psy 152 -------CCGAGGGAATGGAGTTGCTGTCGGGCCCCGGATGTCCCGTTTGCGTTACCTC- chloroflexi-dehalococcoides_et 152 -------CGCCCCATATTGAAATGCTGTCAGGGCCGGGCTGCCCTGTTTGTGTAACCTC- chloroflexi-dehalococcoides_CB 152 -------CGCCCCATATTGAAATGCTGTCAGGACCGGGCTGTCCTGTCTGTGTAACCTC- clostridia-desulfitobacterium_ 161 -------CGGCTCAGATTCGCTTGGTCTCCGGTCCGGGGTGCCCTGTATGTGTTACAGA- Clostr-Moorella_therm 173 -------CAGGTCGCGTAGAATTGCGAAGCGGCCCCGGGTGTCCTGTTTGTGTAACTGA- Bacteroides-Robiginitalea_bif 152 -------CTAAAGAGGTTCAAATGATTCATGGGCCCGGATGTCCGGTTTGTGTTACCCC- epsilon-Helicobactr_acin 170 -------CCAATCATTTAGAGTTTGTGCATGGACCGGGTTGCCCGGTATGCGTGATGCC- epsilon-Helicobacter_pylor 170 -------CTAACAATTTAGAGTTTGTGCATGGGCCGGGCTGCCCGGTATGCGTGATGCC- gamma-PhotobacteriumSKA34 176 -------CTGAGAATATTGATTTTATTCATGGTCCCGGTTGTCCTGTTTGTGTCATGCC- gamma-Vibrio_ang 176 -------CAGAGAATATTGATTTTATTCATGGTCCTGGTTGTCCTGTTTGCGTTATGCC- Gamma-Photobacterium_prof 173 -------CAGGTAATCTCCAGTTTGTACATGGTCCTGGGTGCCCTGTGTGCATTATGCC- epsilon-Wolinella_succ 170 -------CCAAAGAGGTGGAGTTTGTCCATGGGCCTGGGTGCCCTGTTTGCGTGATGCC- epsilon-campylobacer_con 152 -------GAGAGCATATAAACTTCGTCCACGGCCCAGGCTGTCCAGTCTGCGTGATGCC- Aquifex 176 -------AAGGTGTCGTGCGTTTTATCCACGGGCCGGGGTGTCCTGTTTGCGTTATGCC- gamma-Actinobacillus 176 -------CGGAAAGTATCGAATTTATTCACGGTCCTGGTTGTCCGGTATGCGTATTACC- gamma-Manheimia-succ 176 -------CCGAGTCTATCGAATTTATACACGGACCGGGTTGCCCGGTTTGCGTGTTGCC- gamma-Acidobacillus_succinogen 176 -------CGCCGTCCATTGAATTTATTCACGGTCCCGGTTGCCCGGTGTGCGTATTGCC- Cyano-Avar 161 -------CAGCCAACATAGAACTGATTCACGGGCCTGGTTGTCCAGTCTGTGTTATGCC- Cyan-Nostoc7120 161 -------CAGCCAACATAGAACTGATTCACGGGCCTGGTTGTCCAGTTTGTGTTATGCC- Cyan-Nodularia_spu 161 -------CCCCCAACCTAGAACTCATCCACGGGCCTGGTTGTCCAGTATGCGTCATGCC- Cyan-Lyngbya8106 173 -------CTGATAGTATTGAACTGATTCATGGCCCCGGTTGTCCCGTTTGTGTCATGCC- Cano-trichodesmium_ery 173 -------CTAATAATATTGAACTAATACATGGCCCTGGCTGTCCAGTTTGCATTATGCC- clostridia-caldicelluosiruptor 131 -------AAGGATACATCGACTTTATTTCAGGGCCGGGCTGCCCTGTTTGTGTTACGCA- clostridia-clostridium_beij 134 -------ATCCTAATATAAATTTATTGTCAGGACCAGGATGTCCAGTTTGTGTTACTCC- Methanobacteriales-Methanospha 125 -------CTTCTGAAGTTCAAATTGTTGCAGGTCCTGGATGTCCAGTATGTGTGGTACC- Thermococcales-Pyrococcus_abys 125 -------CTTCTGAAGTTCAAATTGTTGCAGGTCCTGGATGTCCAGTATGTGTGGTACC- Methanococcales-Methanocaldoco 149 -------CAGAGAATATAACCGTTGTTCCAGGGCCGGGTTGTCCAGTTTGTGTAACAAC- Methanococcales-Methanococcus_ 161 -------CCGATAATATTACGGTAGTTCCAGGACCCGGATGCCCTGTGTGTGTTACAAC- delta-Lawsonia_intra 152 -------CAAAAGGGATAGATCATCTTTCGGGACCTGGATGTCCTGTATGTGTAACACA- epsilon-campylobacter_jej 152 -------CAAAGGAAATTAACTTTATCCATGGGCCAGGATGTCCTGTTTGTGTTATGCC- epsilon-campylobacter_ups 152 -------CTAAGGAAGTAAATTTCATACACGGACCGGGCTGTCCCGTATGTGTAATGCC- clostridia-clostridium_therm 125 -------CCCCTCGTATACAGCTTATTTCGGGACCGGGCTGCCCTGTATGCGTGACGGC- clostridia-carboxidothermus_hy 146 -------CTCAAAATATCAACCTTATTTCTGGGCCTGGTTGCCCGGTATGTGTAACCGG- Clostr-Halothermothrix_oren 155 -------CAGACAGTATTGAATTAATATCAGGTCCTGGTTGCCCTGTATGTGTAACCCC- Methanosarcinales-Methanosarci 158 -------CTGAGAATATCGAAGTAATAAGCGGTCCCGGATGCCCCGTCTGCGTCACCCC- Methanosarcinales-Methanosarci 158 -------CGAAAAATATCGAGGTAATAAGCGGCCCCGGATGCCCCGTTTGTGTGACTCC- Clostridia-Thermoanaerobacter_ 140 -------CTAAAAACATAAGGCTCATATCAGGACCCGGGTGTCCCGTGTGCGTGACTGC- clostridia-clostridium_cell 155 -------CCCGGCAAATAGAACTGGTTTCAGGGCCGGGATGCCCTGTCTGCGTAACACC- cyano-crocosphaera_wat 152 -------CTCCTGAGATTACCCTGATTCACGGTCCTGGATGTCCTGTTTGTGTTACCCC- Bacteroides_Flavobacteriu_john 152 -------CAAAAAACATTAGGATGATACACGGGCCGGGTTGTCCGGTTTGTGTTACGCC- _____________________ Position of primer hypD-for1 and hypD-for2

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Proteo-others-MagentococcusMC1 186 CGAAGCTGAAATTCGCGCGGCCATGCATTTGGCG---------------GTGCGTGAGGA beta-Nitrospira_multi 183 TGAAGAAGATGTATACCAGGCGATTCAGCTCGCG---------------CTGCGCACCGA beta-Thiobacillus_denitri 180 CGAAGAAGACGTCTACCAGGCGATCCAGCTCGCG---------------CTGCAGGACAG alpha-Acidiphilum 189 CGAGGAAGACCTCGCCGACGCGATCGCCCTGGCG---------------ATGGCCGGCGG Thermococcales-Pyrococcus_fur 204 CGTTGAGGACATAGTGAAGATGATGGAAATTATGAAAGTTGCGAGAGAGGAGAGGGAAGA Thermococcales-Pyrococcus_hori 204 CGTTGAAGATATAGTAAAGATGATGGAGATAATGAAAATTGCAAGGGAAGAGGGGGAAGA Thermococcales-Thermococcus_ko 216 GGTTGAGGACATCGTGGCGATGCAGCTCATCATGAGGAAAGCCCGGGAAGAGGGAGAAGA Archaeoglobales-Archaeoglobus 231 CGACGAGGACATACAGATGGTAATGCACCTGCTG---------------GAGAAGGAAAA Methanopyrales-Methanopyrus_ka 195 TACGGGAGAGCTCGCCGCGGCCATAAAGGCTGCG------------------GAAGATGG Methanomicrobiales-Methanospir 177 ACAGGGAGAGATAGATGCAGCCCTTGAGTTTGCG------------------GAAAAAGG Sulfolobales-Metallosphaera_se 225 TGCTACTGACATAGATGACGCTGTCAAGCTCGCC------------------ATGGATGG Thermoproteales-Pyrobaculum_is 247 TCCTACT-ACCTAGAGGAGGCGATCAAACTGGCC------------------TTTGAAGG Methanobacteriales-Methanother 177 GGCAAGGGAAATCGACGAGTGCATTGAACTTGCA------------------AGGCAGGG Methanomicrobiales-Methanocull 177 -----GC-AGGGCGAGATCGACGCCGCGCTCGAC------------------CTG-GTGG Thermoproteales-Thermofilum_pe 271 TCCTGGT-ACATCGAGAGGGCTATAGAGCTCTCC------------------CTGAGCGG delta-Anaeromyxobacter 228 CGGCCCCGAGGTGGACGAGGCGGTCGCGCTGGCG------------------ATGCAGGG delta-desulfovibrio_des 204 CGAAAGCGAGATAGCCGCCTATCTGGAACTGGCG---------------GAACGCGACAA delta-geobacter_metalli 213 CAATGAGTACCTGGACCGGGCCGTGGCCCTCTGC---------------CGGCTCCCCGA delta-Pelobacter_propion 213 CAACCGCTACATCGACCGGGCAATAGCCATTAGC---------------CGTCTTTCCGA delta-geobacterFRC32 213 AAACCACTATCTGGATCGCGCCATCGCCCTGAGC---------------AGGCTTCCCGA proteo-others-Mariprofundus_fe 210 GGCCGGCCGTATTGAGTCGGCAATCAGACTGGCC---------------GAAGAGGAGCG alpha-Stappia_agg 210 GGTGCGCCGTCTCGATGATGCGGTCGAGCTGGCG---------------CTGAAGACGAA beta-Methylibium_petro 210 GATCGGCCGCATCGACCTGGCGATCAAGCTCGCA---------------CTCGAGCGTGG beta-Ralstonia_metall 210 CATCGGCCGCATCGATCTCGCCATCAAGCTCGCG---------------TTGGAGCGTGG beta-Rhodoferax_ferri 210 GATCGGCCGCATTGACATGGCGATCGACCTGGCA---------------CTGAAGCAAGG beta-dechloromonas_aro 210 GATCGGTCGCGTCGATCAGGCGATCCGCCTGGCG---------------CTCGAACAGGG beta-Azoarcus 210 GGTGGGCCGCATCGACATGGCGATCAAGCTCGCG---------------ATGGAGCGCGC beta-Burkholderia-viet 210 GGTCGGACGCATCGATCTCGCGGTGCAGCTCGCG---------------CTGGAGCGCGG alpha-Magnetospirillum_mag 210 CGTCGGCCGCATCGACGCCGCCATCTGGCTGGCC---------------AAGCAGCCGGG alpha-Bradyrhizobium_jap 219 GGCGAGCCGTATCGACATGGCGATCCGGCTCGCC---------------GAGCGGCCAGA alpha-BradyrhizobiumBTAi1 210 GGCGGGGCGCATCGACATGGCGATTGCGCTGGCC---------------GAGCGTCCCGA alpha-Rhodopseudomonas_palBisA 210 GGTCGGCCGCATCGACATGGCGATCGCGCTGGCC---------------GAGCGGCCGGA alpha-Rhodopseudomonas_palCGA0 210 GGTCGGCCGGATCGACATGGCGATCCAGCTCGCG---------------CAGCGCCCAGA beta-Burkholderia_phy 210 GGTCGGGCGTATCGACAGCGCGATAGAGCTTGCG---------------CTTGAGCATCG alpha-Rhodospirillum_rub11170 219 CGCCGGCCGCATCGACGCCGCCCTGGCTTTGGCC---------------GCCCGGCCCGA beta-Burkholderia_xeno 234 GATGGGACGCGTCGACGACTGTGTGGCGCTCGCC---------------AGGCGGCCCGA alpha-Sphingopyxis_alask 225 GATGGGCCGGGTCGATGATTGCGTGGAACTGGCT---------------ATGAAGCCGGA gamma-Shigella_flex 225 GATGGGTAGAATCGACACCTGCGTGGAGATTGCC---------------AGCCATCCGGA gamma-Shigella_sonn 225 GATGGGTAGAATCGACACCTGCGTGGAGATTGCC---------------AGCCATCCGGA gamma-Shigela_dys 225 GATGGGTAGAATCGACACCTGCGTGGAGATTGCC---------------AGTCATCCGGA gamma-EcoliK12 225 GATGGGTAGAATCGACACCTGCGTGGAGATTGCC---------------AGCCATCCGGA gamma-Ecoli_CFT073 225 AATGGGTAGAATCGACACCTGCGTGGAGATTGCC---------------AGCCATCCGGA gamma-salmonella_ent 225 AATGGGACGCATCGACAGTTGCGTGGAAATCGCC---------------AGCCATCCGGA gamma-salmonella_LT2 225 AATGGGACGCATCGACAGTTGCGTGGAAATCGCC---------------AGCCATCCGGA gamma-enterobacter_638 225 GATGGGCCGTATCGATAATTGCATCGAAATCGCC---------------AGCCAGCCTGA gamma-erwinia_caro 225 GATGGGGCGTATCGACAACTGCCTTGAGATAGCC---------------AGCCATCCAGA gamma-Yersinia_ber 225 GATGGGCCGCATTGATAGCTGTTTGGAAATTGCC---------------GCGCACCCTGA gamma-Yersinia_int 225 GATGGGCCGCATTGATAGCTGTTTGGAAATTGCC---------------GCGCACCCTGA gamma-Yersinia_fred 225 GATGGGGCGTATCGACAGTTGTTTGGAGATTGCT---------------GCTCATCCGGA gamma-Yersinia_moll 225 GATGGGGCGTATCGACAGTTGTCTCGAAATTGCC---------------GCCCATCCCGA gamma-Serratia_protea 225 CATGGGGCGTATAGATGCCTGCTGTGAAATAGCC---------------GCTCACCCGGA alpha-Rhodobacter_sphaer 225 GATGGGCCGGGTCGACGAATGCATCGCCATCGCC---------------GAGCGGCCCGA alpha-Rhodobacter_spaer17025 225 CATGAGCCGGGTGGACGAATGCATCGCGATCGCC---------------GAGCGGCCGGA alpha-Roseovarius-2601 225 GATGAGCCGGGTGGATGAATGCGTCGAGATCGCC---------------GAGCGTCCGGG alpha-Paracoccus_deni 225 GCGTGCGCGGATCGATGAATCCATCCAGATTGCC---------------AGGCGTCCGGG alpha-Xanhobacter_auto 225 CATGGGCCGGGTGGACGATTGCGTCGCCATCGCC---------------GAGCGGGAAGA actinobacteria-corynebacterium 210 CATGGGACGCGTCGACGATGCCTTGTGGTTGGCT---------------TCCCAACCCAA Actinobacteria-RhodococcusRHA1 207 CATGGGCCGTGTCGACGACGCCATGTGGCTGGCC---------------GAACAGCCCGG Chloroflexi-Acidobacteria 204 ACTCGAGTTGATCGACAAAGCTTGCGAGATTGCG---------------ACGCGGCCCGA Chloroflexi-Solibacter_usi 204 GCTAGACATGATCGATCGCGCGCACGCGATCGCG---------------CGGCGGCCCGA delta-proteo_MMS1 204 GGCCTCCCTGATCGACCAGGCCATTGCCCTGGCC---------------GGCCGGCCGGA Cyan-SynechococcusWH5701 204 GGCGGAGACGATCGATCGGGCCCTTCAGCTGGCG---------------GCCCTGCCGGA Cyan-Synechocystis6803 204 GATGGAATTAATTGACCAGGCTTTGTGGTTAGCT---------------AAGCAACCGGA chloroflexi-chloroflexus_agg 204 ACTCGAAATCATCGACAAGGCGCTGGCCATTGCC---------------GCCCTCCCCGA chloroflexi-chloroflexus_aur 204 GCTGGAGATTATCGATAAGGCGTTAGCGATTGCA---------------GCACGGCCCGG Chloroflexi-Roseiflexus_cas 204 GCTGGAGATTATCGACAAGGCGCTGGCAATCGCT---------------TCCCGTCCCAA Chloroflexi-RoseiflexusRS-1 204 GCTGGAGATCATCGACAAGGCGCTGGCAATCGCT---------------TCCCGCCCCAA Actinobacteria-Mycobacterium_g 204 GCTGGAGATCATCGACAAGGCCCTCGATATCGCG---------------TCGCGCCCGGA Actinobacteria-Mycobacterium_v 204 GCTCGAGATCATCGACAAGGCACTCGACATCGCC---------------TCCCGCCCGGA Actinobacteria-FrankiaCcI3 204 GCTTGAGATCATCGACAGGGCGCTGGAGATCGCC---------------GCACGGCCGGG Actinobacteria-FrankiaACN14a 204 GCTGGAGATCATCGACCGGGCGCTCGAGATCGCC---------------TCCCGGCCGGG Actinobacteria-Streptomyces_av 204 GCTGGAGGTCATCGACAAGGCTCTGGAGATCGCC---------------TCCCGGCCGGA Actinobacteria-Mycobacterium_s 204 CCTGGAGGTGATCGACCGTGCGTTGGAGATCGCC---------------GCGCGTGACGA Chlorobi-Pelodictyon_lut 204 GCTCGAGTCCATTGAGCGGGCGCTTCTTCTTGCT---------------TCAAAGAAAGA

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Chlorobi-Pelodicton_lut 204 GCTTGAATCGATTGAGCGGGCTCTTCTTATAGCG---------------GCAAGAAAAGA chlorobi-chlorobium_lim 204 GCTTGAAGTTATCGATCAGGCGCATTCGATTGCA---------------TCGATGCCGGG Chlorobi-Prosthecochloris_aest 204 TCTTGAGATGATTGACCGGGCCCATGCAATAGCC---------------GCTATGCCCGG chlorobi-chlorobium_tep 204 GCTGGAGACTATCGAGCGGGCGCTGGCGATTGCG---------------GCCAGGCCGAA delta-Syntrophus_acid 213 CATCCATGACGTTGACCGAGCCCTGTATCTGGCG---------------GATCGGCCTCA delta-desulfotalea_psy 204 TGCCGCCCATATGGATGCCTTTATCTCCATAGCC---------------GAAAGAGATGG chloroflexi-dehalococcoides_et 204 TACCGCCGACCTGGACAAAGTCATAGCCCTGTCC---------------AGGCTGCCAGG chloroflexi-dehalococcoides_CB 204 TACTGCCGATCTGGACAAAGTCATAGCCCTGTCC---------------AGACTGACCGG clostridia-desulfitobacterium_ 213 CAGCAGCGATATTGATCAGTTTCTCTATCTGGCT---------------GCTCAGCCTCA Clostr-Moorella_therm 225 TCACCAGGAAATCGATATGATGGTCGATCTGGCC---------------CGCCTGCCCGG Bacteroides-Robiginitalea_bif 204 GCTTAATTTAATCGATAAAGCCATCTATCTCGCT---------------GAGGGCAAAAA epsilon-Helicobactr_acin 222 AAGAGTACGCCTGGATGAAGCTTATGAGCTCGCT---------------AGCATGAAAGA epsilon-Helicobacter_pylor 222 AAGAGCGCGCCTTGATGAAGCTTATGAACTCGCT---------------ACTATCAAAGA gamma-PhotobacteriumSKA34 228 CAAAGAGCGTATTGATCATGCCGCCGCACTGGCA---------------TGCCAACCCAA gamma-Vibrio_ang 228 TAAAGAGCGTATTGATCATGCCGCTGCACTGGCA---------------TGCCAACCCAA Gamma-Photobacterium_prof 225 TAAAGAACGGATAGATCAGGCGATTGCATTGGCT---------------CGGTTGCCAAA epsilon-Wolinella_succ 222 CAAAGAGAGAATCAATCAAGCCTATGAGATTGCT---------------CTGCAAGAGGG epsilon-campylobacer_con 204 AAAAAGCCGCATAGACGAGGCCTGTAAGCTTGCT---------------AGTATGGAGAA Aquifex 228 CATGCAGAGGATAGACCTCGCCATAGAGCTTGCA---------------AAAAGGAAAGA gamma-Actinobacillus 228 GATGGGGCGAATTGACGTATGTATTGAAATCGCT---------------CGTCGCCCCGA gamma-Manheimia-succ 228 TATGGGGCGGATTGACCTGTGTATTGAGATTGCA---------------CAAAATCCCAA gamma-Acidobacillus_succinogen 228 GATGGGGCGCATTGATGTGTGTATCGAAATCGCG---------------GAAAAACCGCA Cyano-Avar 213 TAAGGGGAGATTGGATGATGCGATCGCTATTGCT---------------CAAAATCCTAA Cyan-Nostoc7120 213 TAAGGGGAGATTAGATGATGCGATCGCTATCTCC---------------CAAAATCCCAA Cyan-Nodularia_spu 213 AAAAGGGAGATTAGACGATGCGATCGCCATATCC---------------CAAAATCATAA Cyan-Lyngbya8106 225 ACGAGGAAGACTCGATGCTGCTATTGCTTTAGCC---------------GACATTCCTAA Cano-trichodesmium_ery 225 CAGAGGTAGATTAGATGATGCGATCGCTCTGGCA---------------GAAAAACCCAA clostridia-caldicelluosiruptor 183 TGAGGGTTATATTGATAGACTTGTAGAACTTTCC---------------------TCAGA clostridia-clostridium_beij 186 AGAAGGTTATATAGATGCAGCTATAGAATTACTT---------------AAGAATTCAAA Methanobacteriales-Methanospha 177 TTCACATGAAATAGATGAATGTGTGGCATTAGCA---------------GAA---CAAGG Thermococcales-Pyrococcus_abys 177 TTCACATGAAATAGATGAATGTGTGGCATTAGCA---------------GAA---CAAGG Methanococcales-Methanocaldoco 201 TCAAAAAGAGATAGATACAGCCATATATTTAGCT---------------GAC---AATGG Methanococcales-Methanococcus_ 213 ACAAAAAGAAATCGATAAAGCAATGTATCTTGCA---------------GAA---CAGGG delta-Lawsonia_intra 204 TGATAGTGAAATTGAACTTTTTTTAAGGTTTGCA---------------GCTAAACCAAA epsilon-campylobacter_jej 204 AAGAGTTCGCATTGATACAGCTATTAAGCTAGCC---------------TCCATGAAAGA epsilon-campylobacter_ups 204 ACGCTCTCGCATCGACATAGCCTTAAAATTAGCT---------------AGTCAAAAAGA clostridia-clostridium_therm 177 CTCATCCTACATTGACAGGCTTGTAGAATATTCT---------------CTAAAGGACAA clostridia-carboxidothermus_hy 198 AGAGGAAATAATGGTAGAGGCTTTTGAGCTTCTT---------------GAGAAACATAA Clostr-Halothermothrix_oren 207 TGATATATATATTGACAAGGCCCTGGCCCTGGCA---------------AAAAAAGAGAA Methanosarcinales-Methanosarci 210 CGAAAAAGACGTTGACATTGCAATTGCCCTTGCG---------------AAA---AGCGG Methanosarcinales-Methanosarci 210 TGAAGAAGATATTAATATTGCAATTGCCCTTGCG---------------AAA---AGTGG Clostridia-Thermoanaerobacter_ 192 CCAGAACGAAATAGACGCAGTGATAGAGCTTTCA---------------AAT---AAAAA clostridia-clostridium_cell 207 AACGTCTTATATTGACAGTGCGACTGAGCTTGCA---------------GATAGCAAGAA cyano-crocosphaera_wat 204 CATTGAATTAATAGATTGTGCTTTAAAAATTGCT---------------AGTTTACCTAA Bacteroides_Flavobacteriu_john 204 TGTTTCGTTAATCAATAAAGCAATTGAATTAATG---------------AA---ACAAGG

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Proteo-others-MagentococcusMC1 231 TGTGATCTTGGCGGCGTTTGGTGACATGCTGTCGGTACCCATCT----CC-TATGGGGCG beta-Nitrospira_multi 228 TATGATTCTGGTCACTTTCGGTGATATGCTGCGCGTGCCCGTGA----ACGTGCCGAAGA beta-Thiobacillus_denitri 225 GGTCATTCTCGTCGCCTACGGCGACATGCTGCGGGTACCGGTCA----ACGTCAAGAAAG alpha-Acidiphilum 234 CGTCACCCTCGTCGCCTTCGGCGACATGCTGCGCGTGCCGATCA----ACGCGCCGAAGG Thermococcales-Pyrococcus_fur 264 AATTATTCTCACTACTTTTGGTGACATGTATAGAAT-------------TCCAACTCCAA Thermococcales-Pyrococcus_hori 264 GATAATACTTACGACCTTTGGCGATATGTACAAGAT-------------TCCAACTCCAA Thermococcales-Thermococcus_ko 276 GATAATCCTGACGACCTTCGGCGACATGTACAAGAT-------------CCCGACGCCGA Archaeoglobales-Archaeoglobus 276 CATCACCCTCGCCACCTTCGGGGATATGGCGAGAGT-------------CCCCTTTCAGG Methanopyrales-Methanopyrus_ka 237 CATGGTCGTGTGCGCTTTCGGGGACGTGTACAGGGT-------------CCCCACTCCTG Methanomicrobiales-Methanospir 219 GTGCATCATTGCGACATATGGTGATCTGCTCAGGGT-------------GCCTGGTTCAA Sulfolobales-Metallosphaera_se 267 CGTAGTGGTAACGACCTATGGTGACATGAGCAGAG--------------CCAGGGGTACA Thermoproteales-Pyrobaculum_is 288 AGTAGTGATATATACATATGGAGACGTCTTCAGGCTCCCGGCTC----TCCAGAGGGTGA Methanobacteriales-Methanother 219 TGTCACCATAACAACCTTCGGTGACATGCTCCGGGT-------------CCCGGGCTCCA Methanomicrobiales-Methanocull 212 AGCGG--GACTGCAC---CATCGCCACCTACG-GCGACCTGCTG----CGTGTCCCGGGG Thermoproteales-Thermofilum_pe 312 GGTGACCGTTTACACGTACGGGGATACCTTCAAGCTCCGCGCGG----TGAACCCCGTGG delta-Anaeromyxobacter 270 CGTGCGCGTGTGCACGTACGGTGACATGCTCCGGCTC------------CCCGGCACCGC delta-desulfovibrio_des 249 GGTGATCATCGCCACGTTCGGCGACCTTATGCGTGT------------TCCCGGCCCCGG delta-geobacter_metalli 258 CGTTATCGTCGCCACCTTCGGCGACATGGTCCGGGTC------------CCCGGCTCCAC delta-Pelobacter_propion 258 CACCATCATCACCACCTTCGGTGACATGCTGCGGGTT------------CCCGGTTCCAG delta-geobacterFRC32 258 TGTCATTATCGCCACCTTCGGCGATATGATGCGGGTG------------CCTGGTTCATC proteo-others-Mariprofundus_fe 255 TGTAATTCTCTGCACCTATGCCGATCTGATGCGCGTGCCGGCA------------GGCAG alpha-Stappia_agg 255 CGTAATCCTGACCACCTATGGCGACATGATGCGGGTTCCGGGC------------ACCAA beta-Methylibium_petro 255 AGCCATCGTCTGTACCTACGGCGACACCATGCGCGTGCCGGCG------------TCCGA beta-Ralstonia_metall 255 TGTGATCCTCTGCTCCTATGGCGACACGCTGCGCATCCCCGCC------------TCCGA beta-Rhodoferax_ferri 255 CGTGATCCTGTGCACCTATGGCGACACCTTGCGCGTGCCGGCC------------TCTGA beta-dechloromonas_aro 255 CGTCACGCTGTGCACCTACGGCGACTGCCTGCGCGTGCCGGCC------------TCCGA beta-Azoarcus 255 GGTGACGATCTGCACCTACGGCGACTGCCTGCGCGTGCCCGCC------------TCCGA beta-Burkholderia-viet 255 CGTGATCGTCTGCACCTATGGCGACACGATGCGCGTGCCGGCC------------TCCGG alpha-Magnetospirillum_mag 255 CGTCACCCTGGTCACCTATGGCGACATGCTGCGGGTGCCGGGC------------TCGAA alpha-Bradyrhizobium_jap 264 GGTCATTCTGTGCGTCTACGGCGACCTGATGCGCGCGCCTGGG------------TCGCA alpha-BradyrhizobiumBTAi1 255 CATCATCCTCTGCGTCTATGGCGATCTCATGCGCGTGCCGGGA------------TCGCG alpha-Rhodopseudomonas_palBisA 255 GGTGACGCTGTGTACGTACGGCGATCTGATGCGGGTGCCGGGC------------TCGCA alpha-Rhodopseudomonas_palCGA0 255 GGTGACGCTGTGCACCTATGGCGACCTGATGCGGGTGCCCGGT------------TCGCA beta-Burkholderia_phy 255 CGTCACGCTTTGCACGTATGGCGACACGCTACGCGTGCCCGCG------------TCGCG alpha-Rhodospirillum_rub11170 264 GGTGATCTTGTGCAGCTATGGCGATCTGTTCCGCGTGCCCGGT------------TCGGC beta-Burkholderia_xeno 279 AGTGATCTTCACGACGTTCGGCGACGCAATGCGAGTACCGGGC------------TCCAG alpha-Sphingopyxis_alask 270 GGTGATCTTCACTACGTTTGGTGATGCCATGCGCGTGCCGGGA------------TCGCG gamma-Shigella_flex 270 AGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTACCAGGG------------AAACA gamma-Shigella_sonn 270 AGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGG------------AAACA gamma-Shigela_dys 270 AGTCATCTTCTGTACCTTTGGCGACGCCATTCGCGTACCAGGG------------AAACA gamma-EcoliK12 270 AGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGG------------AAACA gamma-Ecoli_CFT073 270 AGTCATCTTCTGTACCTTTGGCGATGCCATGCGCGTACCAGGG------------AAACA gamma-salmonella_ent 270 GGTGATTTTCTGCACCTTTGGCGATGCCATGCGCGTGCCGGGG------------AAACA gamma-salmonella_LT2 270 GGTGATTTTCTGCACCTTTGGCGATGCCATGCGCGTGCCGGGG------------AAACA gamma-enterobacter_638 270 GGTGATTTTTTGCACCTTTGGCGATGCGATGCGCGTGCCGGGC------------AAAAA gamma-erwinia_caro 270 AACGCTTTTTTGTACCTTCGGTGACGCCATCCGTGTTCCGGGG------------AAAAA gamma-Yersinia_ber 270 GGTGATTTTTTGCACTTACGGTGATGCGATGCGTGTGCCGGGG------------CGCAA gamma-Yersinia_int 270 GGTGATTTTTTGCACTTACGGTGATGCGATGCGTGTGCCGGGG------------CGCAA gamma-Yersinia_fred 270 GGTGATTTTCTGCACTTACGGCGATGCCATGCGAGTGCCGGGC------------CGCAA gamma-Yersinia_moll 270 GGTGATTTTCTGCACCTACGGCGACGCCATGCGGGTACCGGGG------------CGCAA gamma-Serratia_protea 270 GGTGATTTTTTGCACCTTCGGCGACGCGTTGCGGGTACCGGGC------------AAACA alpha-Rhodobacter_sphaer 270 GGTGATCTTCACCACCTTCGGCGATGCGATGCGCGTGCCGGGG------------GCGCG alpha-Rhodobacter_spaer17025 270 GGTGATCTTCACCACCTTCGGCGATGCGATGCGCGTGCCGGGA------------ACGCG alpha-Roseovarius-2601 270 CGTCATCTTCACCACCTTCGGCGATGCCATGCGGGTGCCGGGC------------TCGCG alpha-Paracoccus_deni 270 CGTCATCTTCACCACCTTTGGCGATGCGATGAGGGTGCCGGGC------------CGGTC alpha-Xanhobacter_auto 270 GGTGATCTTCGCCACCTTCGGCGACGCCATGCGCGTGCCGGGT------------TCGAA actinobacteria-corynebacterium 255 CGTCATCTTGACCACCTTCGGCGATATGATGCGCGTTCCTGGT------------TCTAC Actinobacteria-RhodococcusRHA1 252 CGTCATCTTCACGACGTTCGGCGACATGATGCGGGTGCCCGGA------------TCCAA Chloroflexi-Acidobacteria 249 CGTCATCTTTTGTTCGTATGGCGACATGCTTCGAGTGCCCGG-------------ATCGC Chloroflexi-Solibacter_usi 249 CGTGATCTTCTGCTCTTTCGGCGACATGCTGCGCGTGCCGGG-------------TTCGG delta-proteo_MMS1 249 GGTGATTTTTACCAGTTTCGGCGATATGTTGCGGGTGCCGGGCAGCCGGGCTGAAAACGG Cyan-SynechococcusWH5701 249 GGTGATCCTCTGCTCCTACGGCGACATGTTGCGGGTGCCGGG-------------AACGG Cyan-Synechocystis6803 249 GATCATTTTTTGTTCCTTTGGCGATATGTTGCGGGTGCCCGGC------------AGTGG chloroflexi-chloroflexus_agg 249 AGTGATCTTCTGCTCATTTGGCGACATGCTGCGCGTGCCAGGT------------AGCCG chloroflexi-chloroflexus_aur 249 TGTCATCTTCTGTTCATTCGGCGACATGCTGCGCGTGCCCGGT------------AGTCG Chloroflexi-Roseiflexus_cas 249 CGTTATTTTCTGTTCGTTTGGCGATATGCTGCGCGTGCCGGGG------------AGTGC Chloroflexi-RoseiflexusRS-1 249 CGTCATCTTCTGCTCATTCGGCGATATGCTGCGCGTGCCGGGG------------AGCGC Actinobacteria-Mycobacterium_g 249 GGTGATCTTCTGCTCGTTCGGCGACATGCTGCGCGTGCCCGGC------------AGCGA Actinobacteria-Mycobacterium_v 249 CGTCGTCTTCTGCTCGTTCGGCGACATGCTGCGGGTGCCCGGC------------AGCGA Actinobacteria-FrankiaCcI3 249 CGTCATCTTCTGCTCCTTCGGCGACATGCTGCGGGTGCCGGGG------------AGCGA Actinobacteria-FrankiaACN14a 249 GGTCATCTTCTGCTCGTTCGGTGACATGCTGCGGGTTCCCGGT------------AGCGG Actinobacteria-Streptomyces_av 249 GGTGATCTTCTGCTCCTTCGGTGACATGCTCCGTGTGCCCGGC------------ACCGG Actinobacteria-Mycobacterium_s 249 CGTGATCTTCTGCTCGTTCGGCGACATGCTGCGTGTGCCAGGA------------AGTCA Chlorobi-Pelodictyon_lut 249 GGTGATCTTTACAAGCTTCGGCGATATGCTTCGCGTTCCGGGC------------AGCTC

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Chlorobi-Pelodicton_lut 249 GGTGATCTTCACCAGTTTCGGCGATATGCTGCGCGTGCCTGGC------------AGTTC chlorobi-chlorobium_lim 249 GGTGATTTTCGCTTCTTTCGGGGATATGCTGAGGGTTCCGGGC------------AGTGT Chlorobi-Prosthecochloris_aest 249 TGTTATTTTTACAACGTTTGGCGATATGCTTCGTGTGCCGGGC------------AGTCA chlorobi-chlorobium_tep 249 TACGATTCTGACGAGCTTCGGCGACATGCTGCGCGTGCCGGGC------------AACTC delta-Syntrophus_acid 258 TGTCGTCTTCGCCACCTTCGGCGACATGCTCCGCGTTCCCGGA------------ACGGG delta-desulfotalea_psy 249 GGTACGGGTCGCCATCTTCGGTGATCTCTTCCGGGTACCGGGA------------AGCCA chloroflexi-dehalococcoides_et 249 GGTTATAATCTGCTCATTCGGAGACCTGCTAAGGGTTCCCTCC------------AGCCG chloroflexi-dehalococcoides_CB 249 GGTCATCATCTGCTCATTCGGTGATCTTTTGCGGGTTCCCTCC------------AGCCG clostridia-desulfitobacterium_ 258 TGTCATTACCGCAACCTTTGGCGATATGATTCGGGTGCCCGGC------------TCGCA Clostr-Moorella_therm 270 GGTTACGCTGGGAACCTTCGGGGATATGTTACGCGTTCCCGGC------------AGCCG Bacteroides-Robiginitalea_bif 249 TGTCATTCTTTGTTCTTTTGGAGATATGCTACGTGTGCCCGGA------------TCGAA epsilon-Helicobactr_acin 267 TAGCGTTATTTTAAGTTTAGGGGATATGATGAAAGTCCCTGGG------------AGCTA epsilon-Helicobacter_pylor 267 TAGCATTGTTTTGAGTTTAGGGGATATGATGAGAGTCCCCGGG------------AGCTA gamma-PhotobacteriumSKA34 273 CGTTATTTTAGTTACCCTTGGCGATATGATCCGCGTTCCGGGT------------TCAAA gamma-Vibrio_ang 273 CGTTATTTTGGTTACCCTTGGAGATATGATCCGCGTTCCAGGC------------TCAAA Gamma-Photobacterium_prof 270 TGTTGTGCTGGTTACTCTTGGCGATATGATTCGAGTGCCTGGT------------TCGAA epsilon-Wolinella_succ 267 GGTGATTCTTGTCACCTTGGGAGATATGATCAAAGTCCCGGGC------------TCTCA epsilon-campylobacer_con 249 TGTGATCTTTTGCACGCTAGCTGATATGCTAAGAGTGCCTGGT------------TCAAA Aquifex 273 CGTCATTATGTGCTCCTACGGAGACCTCCTGAGGGTTCCGGGC------------TCGAA gamma-Actinobacillus 273 GGTCATTTTTTGTACCTTCGGTGATGCGATGAGAGTAAAAGGC------------AAACT gamma-Manheimia-succ 273 TGTGATTTTTTGCACTTTTGGCGATGCCATGCGGGTAAAAGGG------------CGTAA gamma-Acidobacillus_succinogen 273 TGTGATTTTCTGCACCTTCGGTGACGCTATGCGGGTGAAAGGC------------CGTAA Cyano-Avar 258 CGTCATTTTAGCAACCTTTGGCGACACCATGCGCGTCCCCGGT------------TCCAA Cyan-Nostoc7120 258 TGTCATTTTAACAACATTTGGCGACACCATGCGCGTCCCTGGT------------TCCAA Cyan-Nodularia_spu 258 CGTCATTCTCGCCACATTTGGCGACACCATGAGAGTACCCGGT------------TCCCA Cyan-Lyngbya8106 270 TGTTATTTTTACCACCTTTGGCGATGCGATGCGGGTGCCAGGT------------TCACA Cano-trichodesmium_ery 270 TGTCATTTTTACAACTTTCGGTGATGGGATGCGAGTACCGGGT------------TCTCA clostridia-caldicelluosiruptor 222 GTGGACAATCTATACCTTTGGTGATTTGCTGAAAGTGCCAGGA------------AGTTC clostridia-clostridium_beij 231 TGTGATTTTAAGTACTTTTGGGGATATGATGAGAGTAAATGGA------------AGTAG Methanobacteriales-Methanospha 219 AGTAACAGTTACAATATTTGGGGATATGTTACGCGTACCAGGT------------ACAGA Thermococcales-Pyrococcus_abys 219 AGTAACAGTTACAATATTTGGGGATATGTTACGCGTACCAGGT------------ACAGA Methanococcales-Methanocaldoco 243 ATATGTAATAACCACTCTTGGAGATATGTATAGAGTGCCGGGA------------AGTGA Methanococcales-Methanococcus_ 255 ATATACTATTGCAACGTTAGGAGATATGTATAGGGTCCCAGGA------------AGTAA delta-Lawsonia_intra 249 TGTAATTATTGCTACTTTTGGTGATCTTATGCGAGTACCTGGTCCT---------AATAG epsilon-campylobacter_jej 249 TACGATTTTTTGTACCTTAGGGGATCTTTTAAGGGTGCCTGGA------------AGTGA epsilon-campylobacter_ups 249 TGTGATTTTTTGCGCTTTGGGAGATTTATTAAGAATTCCAGGT------------AGTGA clostridia-clostridium_therm 222 CCATTGTGTTTTGACTTTCGGTGACATGATGAAGGTAAAGGGA------------CACAA clostridia-carboxidothermus_hy 243 CGTTACCCTATTTACTTTTGGGGATATGCTGAAAGTTCCCGGA------------GAGGG Clostr-Halothermothrix_oren 252 TGTAATTATTACTACTTTTGCTGATCTTTTAAGGGTTCCAGGG------------TCTTC Methanosarcinales-Methanosarci 252 AGCAACTGTAGTCACTTTCGGAGATATGATGAGGGTTCCCGGT------------TCAGC Methanosarcinales-Methanosarci 252 GGCAACGGTTGTCACTTTTGGAGACATGATGCGGGTTCCGGGT------------TCGGC Clostridia-Thermoanaerobacter_ 234 TATAGTGATTGCCACTTTTGGGGATTTAATAAGAGTTCCAGGG------------AATAA clostridia-clostridium_cell 252 TGTAATCATTACTACCTTTGGGGATATGATGAGAGTTCCTGGA------------AACAA cyano-crocosphaera_wat 249 TGTTATTTTTTGTTCTTTTGGGGATATGTTGCGAGTCCCTGGA------------AGCGA Bacteroides_Flavobacteriu_john 246 TGTCGTTCTGTGTTCTTTTGGAGATATGATTCGGGTTCCCGGT------------TCATC

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Proteo-others-MagentococcusMC1 286 GCGGGCTA---------------------------------------------------- beta-Nitrospira_multi 284 AAGAAGTG---------------------------------------------------- beta-Thiobacillus_denitri 281 GCGAAATT---------------------------------------------------- alpha-Acidiphilum 290 GCGAGGT----------------------------------------------------- Thermococcales-Pyrococcus_fur 311 TAG--------------------------------------------------------- Thermococcales-Pyrococcus_hori 311 GGG--------------------------------------------------------- Thermococcales-Thermococcus_ko 323 TGG--------------------------------------------------------- Archaeoglobales-Archaeoglobus 323 GGA--------------------------------------------------------- Methanopyrales-Methanopyrus_ka 284 TCG--------------------------------------------------------- Methanomicrobiales-Methanospir 266 ------------------------------------------------------------ Sulfolobales-Metallosphaera_se 313 AAA--------------------------------------------------------- Thermoproteales-Pyrobaculum_is 344 GGGGGGC----------------------------------------------------- Methanobacteriales-Methanother 266 GGG--------------------------------------------------------- Methanomicrobiales-Methanocull 262 TCGAAGG----------------------------------------------------- Thermoproteales-Thermofilum_pe 368 ACGGCGT----------------------------------------------------- delta-Anaeromyxobacter 318 GCGC-------------------------------------------------------- delta-desulfovibrio_des 297 TGG--------------------------------------------------------- delta-geobacter_metalli 306 GTC--------------------------------------------------------- delta-Pelobacter_propion 306 TTC--------------------------------------------------------- delta-geobacterFRC32 306 CAG--------------------------------------------------------- proteo-others-Mariprofundus_fe 303 CCGCG------------------------------------------------------- alpha-Stappia_agg 303 ACAGA------------------------------------------------------- beta-Methylibium_petro 303 CGGCC------------------------------------------------------- beta-Ralstonia_metall 303 GAGCC------------------------------------------------------- beta-Rhodoferax_ferri 303 AGGCC------------------------------------------------------- beta-dechloromonas_aro 303 TGGTC------------------------------------------------------- beta-Azoarcus 303 CGGCC------------------------------------------------------- beta-Burkholderia-viet 303 CGGGA------------------------------------------------------- alpha-Magnetospirillum_mag 303 GCGCC------------------------------------------------------- alpha-Bradyrhizobium_jap 312 AGGGC------------------------------------------------------- alpha-BradyrhizobiumBTAi1 303 CGGGT------------------------------------------------------- alpha-Rhodopseudomonas_palBisA 303 CGGCA------------------------------------------------------- alpha-Rhodopseudomonas_palCGA0 303 TGGCA------------------------------------------------------- beta-Burkholderia_phy 303 TCAGA------------------------------------------------------- alpha-Rhodospirillum_rub11170 312 GCGGC------------------------------------------------------- beta-Burkholderia_xeno 327 CACG-------------------------------------------------------- alpha-Sphingopyxis_alask 318 GCTC-------------------------------------------------------- gamma-Shigella_flex 318 GGGA-------------------------------------------------------- gamma-Shigella_sonn 318 GGGA-------------------------------------------------------- gamma-Shigela_dys 318 GGGA-------------------------------------------------------- gamma-EcoliK12 318 GGGA-------------------------------------------------------- gamma-Ecoli_CFT073 318 GGGG-------------------------------------------------------- gamma-salmonella_ent 318 GGGT-------------------------------------------------------- gamma-salmonella_LT2 318 GGGT-------------------------------------------------------- gamma-enterobacter_638 318 AGGC-------------------------------------------------------- gamma-erwinia_caro 318 TGGC-------------------------------------------------------- gamma-Yersinia_ber 318 TGGC-------------------------------------------------------- gamma-Yersinia_int 318 TGGC-------------------------------------------------------- gamma-Yersinia_fred 318 TGGT-------------------------------------------------------- gamma-Yersinia_moll 318 TGGC-------------------------------------------------------- gamma-Serratia_protea 318 AGGC-------------------------------------------------------- alpha-Rhodobacter_sphaer 318 CGGC-------------------------------------------------------- alpha-Rhodobacter_spaer17025 318 GGGC-------------------------------------------------------- alpha-Roseovarius-2601 318 CAAG-------------------------------------------------------- alpha-Paracoccus_deni 318 GCAA-------------------------------------------------------- alpha-Xanhobacter_auto 318 GAAG-------------------------------------------------------- actinobacteria-corynebacterium 303 CGGC-------------------------------------------------------- Actinobacteria-RhodococcusRHA1 300 GGGC-------------------------------------------------------- Chloroflexi-Acidobacteria 296 ACA--------------------------------------------------------- Chloroflexi-Solibacter_usi 296 AGG--------------------------------------------------------- delta-proteo_MMS1 309 GGACACCATACCTATTTTTCATACGGCCAAAGGGGGGAAGAGGGACACAGCTGCCATGAA Cyan-SynechococcusWH5701 296 G----------------------------------------------------------- Cyan-Synechocystis6803 297 GG---------------------------------------------------------- chloroflexi-chloroflexus_agg 297 CAA--------------------------------------------------------- chloroflexi-chloroflexus_aur 297 CAA--------------------------------------------------------- Chloroflexi-Roseiflexus_cas 297 GAA--------------------------------------------------------- Chloroflexi-RoseiflexusRS-1 297 CAA--------------------------------------------------------- Actinobacteria-Mycobacterium_g 297 GAA--------------------------------------------------------- Actinobacteria-Mycobacterium_v 297 GAA--------------------------------------------------------- Actinobacteria-FrankiaCcI3 297 TCG--------------------------------------------------------- Actinobacteria-FrankiaACN14a 297 CCG--------------------------------------------------------- Actinobacteria-Streptomyces_av 297 TCG--------------------------------------------------------- Actinobacteria-Mycobacterium_s 297 TCA--------------------------------------------------------- Chlorobi-Pelodictyon_lut 297 CCG---------------------------------------------------------

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Chlorobi-Pelodicton_lut 297 AAA--------------------------------------------------------- chlorobi-chlorobium_lim 297 CAG--------------------------------------------------------- Chlorobi-Prosthecochloris_aest 297 TCA--------------------------------------------------------- chlorobi-chlorobium_tep 297 GAA--------------------------------------------------------- delta-Syntrophus_acid 306 CGGCA------------------------------------------------------- delta-desulfotalea_psy 297 AGGG-------------------------------------------------------- chloroflexi-dehalococcoides_et 297 CCAA-------------------------------------------------------- chloroflexi-dehalococcoides_CB 297 CCAA-------------------------------------------------------- clostridia-desulfitobacterium_ 306 AAAG-------------------------------------------------------- Clostr-Moorella_therm 318 GTCT-------------------------------------------------------- Bacteroides-Robiginitalea_bif 297 AAAG-------------------------------------------------------- epsilon-Helicobactr_acin 315 TGGGA------------------------------------------------------- epsilon-Helicobacter_pylor 315 TGGGA------------------------------------------------------- gamma-PhotobacteriumSKA34 321 AGGTA------------------------------------------------------- gamma-Vibrio_ang 321 AGGTA------------------------------------------------------- Gamma-Photobacterium_prof 318 AAGTA------------------------------------------------------- epsilon-Wolinella_succ 315 AGGGT------------------------------------------------------- epsilon-campylobacer_con 297 GACAA------------------------------------------------------- Aquifex 321 AAGGAAG----------------------------------------------------- gamma-Actinobacillus 321 AGGC-------------------------------------------------------- gamma-Manheimia-succ 321 AGGT-------------------------------------------------------- gamma-Acidobacillus_succinogen 321 AGGT-------------------------------------------------------- Cyano-Avar 306 AACC-------------------------------------------------------- Cyan-Nostoc7120 306 AACC-------------------------------------------------------- Cyan-Nodularia_spu 306 TACC-------------------------------------------------------- Cyan-Lyngbya8106 318 AAAA-------------------------------------------------------- Cano-trichodesmium_ery 318 AAAA-------------------------------------------------------- clostridia-caldicelluosiruptor 270 AAAA-------------------------------------------------------- clostridia-clostridium_beij 279 AGAA-------------------------------------------------------- Methanobacteriales-Methanospha 267 AAAG-------------------------------------------------------- Thermococcales-Pyrococcus_abys 267 AAAG-------------------------------------------------------- Methanococcales-Methanocaldoco 291 AAAA-------------------------------------------------------- Methanococcales-Methanococcus_ 303 AAAA-------------------------------------------------------- delta-Lawsonia_intra 300 GAAA-------------------------------------------------------- epsilon-campylobacter_jej 297 AATT-------------------------------------------------------- epsilon-campylobacter_ups 297 AGAA-------------------------------------------------------- clostridia-clostridium_therm 270 ACTT-------------------------------------------------------- clostridia-carboxidothermus_hy 291 GCGA-------------------------------------------------------- Clostr-Halothermothrix_oren 300 TGGA-------------------------------------------------------- Methanosarcinales-Methanosarci 300 GGAA-------------------------------------------------------- Methanosarcinales-Methanosarci 300 TGAA-------------------------------------------------------- Clostridia-Thermoanaerobacter_ 282 GTCC-------------------------------------------------------- clostridia-clostridium_cell 300 CAGT-------------------------------------------------------- cyano-crocosphaera_wat 297 AAAA-------------------------------------------------------- Bacteroides_Flavobacteriu_john 294 AAAA--------------------------------------------------------

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Proteo-others-MagentococcusMC1 294 ---------------------TGCCACCTTGGATGGTGCGCGGCAAGATG---------- beta-Nitrospira_multi 292 ---------------------CGATCCCTGGAGCAGGCCAAGGCAGCA-G---------- beta-Thiobacillus_denitri 289 ---------------------CGATCACT-GGCCGAGGCGCAGGCCGCGG---------- alpha-Acidiphilum 297 ---------------------CGCCTCGCTCGCCGCCGCCCGCGCCGCCG---------- Thermococcales-Pyrococcus_fur 314 -----------------------GAAGCTTTGCAGACTTAAAGAGTCAGG---------- Thermococcales-Pyrococcus_hori 314 -----------------------GAAGTTTTGCAGACCTAAAGAGCCAAG---------- Thermococcales-Thermococcus_ko 326 -----------------------GGAGCTTCGCCGACTTAAAGAGTGAGG---------- Archaeoglobales-Archaeoglobus 326 -----------------------AGAGCCTATTCACTTTAAGGGCAGAGG---------- Methanopyrales-Methanopyrus_ka 287 -----------------------GATCCCTCTC----CTCATGCGATGGG---------- Methanomicrobiales-Methanospir 266 ------------------------ATGTTTCAC-----TGGAAAGCTCTG---------- Sulfolobales-Metallosphaera_se 316 ----------------------ATGTCTCTAGAGGAGGCTAGAGCTATGG---------- Thermoproteales-Pyrobaculum_is 351 ---------------------CAGGTCGCTGGCTGAGGCTAAGGCGATGG---------- Methanobacteriales-Methanother 269 -----------------------GATCCCTCGCGGATGCCAGGGCGGACG---------- Methanomicrobiales-Methanocull 269 ---------------------GC--TCGCTCGAG-----TCGAGCGGCGG---------- Thermoproteales-Thermofilum_pe 375 ---------------------CTCGTCCCTCGAGGAGGCTAGGGCGGCGG---------- delta-Anaeromyxobacter 322 -------------------------TCGCTCGCCGACGCGCAGGCCGACG---------- delta-desulfovibrio_des 300 ---------------------CAAAACACTTAAAAGCGCACAGGCCGCCG---------- delta-geobacter_metalli 309 ------------------------GTCCCTCCAGGAGGAGCGCGCCCGGG---------- delta-Pelobacter_propion 309 ------------------------GTCACTGATGGAGGAGCGCGCCAGGG---------- delta-geobacterFRC32 309 ------------------------TTCTCTCATGGAGGAACGGGCCAGTG---------- proteo-others-Mariprofundus_fe 308 -----------------------ACAGCCTGCACAAGGCAAAAGCACGCG---------- alpha-Stappia_agg 308 -----------------------AAAGCCTGATCCGCGCCAAGGCCGAAG---------- beta-Methylibium_petro 308 -----------------------TGTCGCTGGTTCGTGCCAAGGCGCGCG---------- beta-Ralstonia_metall 308 -----------------------TGTCGCTGCTCAAGGCCAAGGCCCGCG---------- beta-Rhodoferax_ferri 308 -----------------------TGTCGCTGATGAAGGCCAAGGCGCGCG---------- beta-dechloromonas_aro 308 -----------------------TATCGCTGATGAAAGCCAAGGCCAAGG---------- beta-Azoarcus 308 -----------------------TCTCGCTACTGAAGGCGCGCGCCCACCTGGATAAATC beta-Burkholderia-viet 308 -----------------------TGTCGCTGATGCGCGCGAAGGCGCACG---------- alpha-Magnetospirillum_mag 308 -----------------------TGTCGCTGCTGAAGGCCAAGGCCGAGG---------- alpha-Bradyrhizobium_jap 317 -----------------------AGTCCCTGTTGCTTGCCAAAGCGCTGG---------- alpha-BradyrhizobiumBTAi1 308 -----------------------CCTCGCTGCTCAAGGCGAAAGCGCGGG---------- alpha-Rhodopseudomonas_palBisA 308 -----------------------CCTCGCTGCTGCGCGCCAAAGCCGCCG---------- alpha-Rhodopseudomonas_palCGA0 308 -----------------------CCTCGCTGCTGCGCGCCAAGGCGGCCG---------- beta-Burkholderia_phy 308 -----------------------TGAGCCTGTACAAGGCGCGGGCCGCAG---------- alpha-Rhodospirillum_rub11170 317 -----------------------GCAGCATGATCTCGGCCCGGGCCGAGG---------- beta-Burkholderia_xeno 331 -------------------------AGCTTGCTGAAGGCCAAGGCCGATG---------- alpha-Sphingopyxis_alask 322 -------------------------AGCCTGCTAGGCGCAAAGGCCGAAG---------- gamma-Shigella_flex 322 -------------------------TCGCTGTTGCAGGCAAAAGCACGCG---------- gamma-Shigella_sonn 322 -------------------------TCGCTGTTGCAGGCAAAAGCACGCG---------- gamma-Shigela_dys 322 -------------------------TCGCTGTTGCAGGCAAAAGCACGCG---------- gamma-EcoliK12 322 -------------------------TCGCTGTTGCAGGCAAAAGCACGCG---------- gamma-Ecoli_CFT073 322 -------------------------TCGCTGTTGCAAGCAAAAGCACGCG---------- gamma-salmonella_ent 322 -------------------------TCTCTGTTGCAGGCTAAAGCGCGCG---------- gamma-salmonella_LT2 322 -------------------------TCTCTGTTGCAGGCTAAAGCGCGCG---------- gamma-enterobacter_638 322 -------------------------TCACTGTTACAGGCCAGAGCGCGCG---------- gamma-erwinia_caro 322 -------------------------TCTTTACTGGATGCCCGCGCCCACG---------- gamma-Yersinia_ber 322 -------------------------TCGATGCTGGATGCTAAACGGCGCG---------- gamma-Yersinia_int 322 -------------------------TCGATGCTGGATGCTAAACGGCGCG---------- gamma-Yersinia_fred 322 -------------------------TCAATGCTGGATGCCAAGCGGCGCG---------- gamma-Yersinia_moll 322 -------------------------TCGATGCTGGATGCCAAACGGCGCG---------- gamma-Serratia_protea 322 -------------------------TCTTTACTGCAGGCCCGTGAGCGCG---------- alpha-Rhodobacter_sphaer 322 -------------------------TCGCTCTTGCAGGCCAAGGCACGCG---------- alpha-Rhodobacter_spaer17025 322 -------------------------TCGCTTCTGCAGGCCAAGGCGCGGG---------- alpha-Roseovarius-2601 322 -------------------------TCGCTGATGCAGGCCAAGGCCGATG---------- alpha-Paracoccus_deni 322 -------------------------TCGCTGTTGCAGGCGCGGGCGGCGG---------- alpha-Xanhobacter_auto 322 -------------------------AGCCTGCTCCAGGCCAAGGCGGACG---------- actinobacteria-corynebacterium 307 -------------------------TCGCTTCTCGACGCCCGCGCGAACG---------- Actinobacteria-RhodococcusRHA1 304 -------------------------AATCTCATCGAGGCCAAGGCCCGCG---------- Chloroflexi-Acidobacteria 299 -----------------------CAGATTTGTTCACCGTGAAAGCCAGGG---------- Chloroflexi-Solibacter_usi 299 -----------------------GCGATCTCTTCCAGGTGCGGGCGGCGG---------- delta-proteo_MMS1 369 AAATAGGTATGGTGTCCCCGTTTTTAGCCTGCAGCAGGCCAGGGCCGCCG---------- Cyan-SynechococcusWH5701 297 ------------------CCCTTCCCACCTGCTCGAAGCCCGCGCCAGCG---------- Cyan-Synechocystis6803 299 -----------------------CGGATTTGCTGAGCATTAAAGCCCAGG---------- chloroflexi-chloroflexus_agg 300 ------------------------AGACCTCTTCCGCGTCAAGAGCGAAG---------- chloroflexi-chloroflexus_aur 300 ------------------------AGACCTCTTTCGGGTCAAGAGCGAAG---------- Chloroflexi-Roseiflexus_cas 300 ------------------------AGACCTGTTCCGCGTCAAGAGCGAAG---------- Chloroflexi-RoseiflexusRS-1 300 ------------------------AGACCTGTTTCGCGTGAAGAGCGAAG---------- Actinobacteria-Mycobacterium_g 300 ------------------------GGACCTCTTCCGGGTCAAGAGTGAGG---------- Actinobacteria-Mycobacterium_v 300 ------------------------GGACCTGTTCCGCGTCAAGAGCGAGG---------- Actinobacteria-FrankiaCcI3 300 ------------------------GGACCTCTTCCGGGTGAAGAGCGCGG---------- Actinobacteria-FrankiaACN14a 300 ------------------------GGACCTGTTCCGGGTCAAGAGCACCG---------- Actinobacteria-Streptomyces_av 300 ------------------------CGACCTGTTCCAGGTACGCAGCGAAG---------- Actinobacteria-Mycobacterium_s 300 ------------------------GGACCTGTTCAGCGTCAAGGCCCGCG---------- Chlorobi-Pelodictyon_lut 300 ------------------------TGACCTCTTCATGGTTCGCAGCGAAG----------

! 30

Chlorobi-Pelodicton_lut 300 ------------------------AGATCTCTTTATGGTGCGCAGTGAAG---------- chlorobi-chlorobium_lim 300 ------------------------CGATCTTTTCACGGTTCGCAGCAGGG---------- Chlorobi-Prosthecochloris_aest 300 ------------------------GGACTTTTTTTCCGTACGCAGCCGGG---------- chlorobi-chlorobium_tep 300 ------------------------AGACCTGTTCATGGCGCGGAGCGAGG---------- delta-Syntrophus_acid 311 -----------------------GAAGTCTCCAGCGGCTTCGCGCGGCAG---------- delta-desulfotalea_psy 301 -------------------------TCGCTAGCCAACGCCAGTTCCCGCG---------- chloroflexi-dehalococcoides_et 301 -------------------------AGCCTGCTGGAAGTGCGGGCGGAAG---------- chloroflexi-dehalococcoides_CB 301 -------------------------AGCCTGCTGGAAGTGCGGGCGGAAG---------- clostridia-desulfitobacterium_ 310 -------------------------AGTCTGCAGATGCTGCGTGGGGAAG---------- Clostr-Moorella_therm 322 -------------------------TCCCTGGAGAGGGAACGCGCCGGCG---------- Bacteroides-Robiginitalea_bif 301 -------------------------AGTTTGCTCCAGGCCAAAGCTGGTG---------- epsilon-Helicobactr_acin 320 --------------------------GTTTGATAAAAGCGAGGGAAAAGG---------- epsilon-Helicobacter_pylor 320 --------------------------GTTTGATACAAGCCAGAGAAAAGG---------- gamma-PhotobacteriumSKA34 326 --------------------------GTTTGGCGCAATATCGTGCTAAAG---------- gamma-Vibrio_ang 326 --------------------------GTTTGGCGCAATATCGTGCTAAAG---------- Gamma-Photobacterium_prof 323 --------------------------CTCTGGCTCAGTGCCGTGCTGAGG---------- epsilon-Wolinella_succ 320 --------------------------CGCTCCAGAGCGCTAGAGCTCTAG---------- epsilon-campylobacer_con 302 --------------------------GTTTGCAAAAGCTTCGCGGCGAGG---------- Aquifex 328 -------------------------AGCCTACTCAACGTCCGGGCGGAAG---------- gamma-Actinobacillus 325 -------------------------TCTTTATTGGATGCTAAAGGACAGG---------- gamma-Manheimia-succ 325 -------------------------TCCTTGCTGGAAGCAAAAGCCCAGG---------- gamma-Acidobacillus_succinogen 325 -------------------------TCCTTGCTGGAAGCCAAAGCGCGCG---------- Cyano-Avar 310 -------------------------AGCTTGCTGCAAGCCAAAGCCACAG---------- Cyan-Nostoc7120 310 -------------------------ACCTTGCTGCAAGCCAAAGCCCAAG---------- Cyan-Nodularia_spu 310 -------------------------AACCTCCTCCAAGCCAAAGCCCAAG---------- Cyan-Lyngbya8106 322 -------------------------AGTTTGCTACAAGCCAAAGCCCAAG---------- Cano-trichodesmium_ery 322 -------------------------AGTTTGTTACAGGCAAAAGCTCAGG---------- clostridia-caldicelluosiruptor 274 -------------------------TCGCTTTCACAAGCACGGGCAAATG---------- clostridia-clostridium_beij 283 -------------------------A-ATTTAATTAAGCAAAGAGAAAAA---------- Methanobacteriales-Methanospha 271 -------------------------TCATTAGCCGATGCTAAAGCAGAAG---------- Thermococcales-Pyrococcus_abys 271 -------------------------TCATTAGCCGATGCTAAAGCAGAAG---------- Methanococcales-Methanocaldoco 295 -------------------------TCTTTGATGGAAAAGCAATCTGAGG---------- Methanococcales-Methanococcus_ 307 -------------------------TCATTAATGCAACTTCAATCGGAAG---------- delta-Lawsonia_intra 304 -------------------------TCACTTAAGCATGCACAAGCAGAGG---------- epsilon-campylobacter_jej 301 -------------------------TCTTTACTGGATTTAAGAGCTAAAG---------- epsilon-campylobacter_ups 301 -------------------------TCCCTACTTAATTTGCGTGCTAGGG---------- clostridia-clostridium_therm 274 -------------------------TCTCTTACCGAGGCAAAAGCAACGG---------- clostridia-carboxidothermus_hy 295 -------------------------ACTTTAAGTATTTTAAAAGCCCAGG---------- Clostr-Halothermothrix_oren 304 -------------------------ACACTGCAGGAAATGAGAGTTAACA---------- Methanosarcinales-Methanosarci 304 -------------------------AGCCTTCTGGATGCAAAAGCAGAAG---------- Methanosarcinales-Methanosarci 304 -------------------------AGCTTACTGGATGCAAAGTCTGAAG---------- Clostridia-Thermoanaerobacter_ 286 -------------------------TCCCTTCAGAAGGAAAGAGCTAAAG---------- clostridia-clostridium_cell 304 -------------------------ACGCTACTTCACAAAAAGGCTCAAG---------- cyano-crocosphaera_wat 301 -------------------------GATTTATTAACTATCAAAGCTTCTG---------- Bacteroides_Flavobacteriu_john 298 -------------------------AGTCTATTGCAGGCCAAAGCTGAAG----------

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Proteo-others-MagentococcusMC1 323 --GCGCG-GATGTGGTTGCTGTGGCCAATCCTCATGAGGCGGTCGAGCTGGCCTTACGTC beta-Nitrospira_multi 320 --GAGCG-GATGTGCGTCCCATTGCCAGTCCGCGCGAAGCAGTCAGGATAGCTCAGCAAA beta-Thiobacillus_denitri 317 --GGGCG-GACGTGCGGCCGATCGCGAGCCCGCTCGACGCCCGCAGGATCGCAGCCGAAA alpha-Acidiphilum 326 --GCGCG-GATGTGCGCCCCATCGCCGCGCCGCAGGACGCGGTGGCGATCGCCCGCGCCG Thermococcales-Pyrococcus_fur 341 --GTTAC-GATGTGAGGATAGTTTACTCTATATACGACTCCTATAAAATAGCCAAGGAAA Thermococcales-Pyrococcus_hori 341 --GTTAC-GACGTGAGGATAGTCTATTCAATATATGACTCCTATAAAATAGCTAAGGAAA Thermococcales-Thermococcus_ko 353 --GCTTT-GATGTCAGAATCGTCTATGGTATCTTTGACACCTACAGAATCGCCAAGGAGA Archaeoglobales-Archaeoglobus 353 --GGTAT-GACATAAGAATCGTTTACAGCATCTTTGACTCCTTAAAGCTTGC---AGAAA Methanopyrales-Methanopyrus_ka 310 --G------ACGTCAGGGTGGTCCAGAGCGTTAGGAAGGCCGAGGAGATCGCC---GAGT Methanomicrobiales-Methanospir 287 --GAGCC-GATGTCCGTATTGTTCAGGGTGTGCATAAGGCTGTTGAGATAGC---AAAGA Sulfolobales-Metallosphaera_se 344 --GAGGA-GATGTGAGAGTGATATATGGGGTGCAAGATGCGGTGAAGATGGCTAGAAGGG Thermoproteales-Pyrobaculum_is 380 --GTGCC-GACGTGAGACTTATACACACCTTCCTCCACGCGGCTATAGACGCCAAGAGGC Methanobacteriales-Methanother 296 --GTGCG-GATGTCAGGATAGTCTACGGTGTGGGCAACGCAGTGGAGATAGC---AAGGA Methanomicrobiales-Methanocull 291 --AGAC-----GTCCGGGTGGTGCAGGGCGTCCACAAGGCGGTGGAGATCGCCCGGCGGG Thermoproteales-Thermofilum_pe 404 --GGGGT-AGGGTGAGGCTCGTGGTGAGCTTAGTGGACGCCGTGAGGGACGCCTCTAGGC delta-Anaeromyxobacter 347 --GCGGC-AAGGTCGAGGTGGTCTACTCGATCTCGCAGGCGGTCGAGCTCGCCCAGGCGC delta-desulfovibrio_des 329 --GTGCC-CGCATCGAAGTGGTCTATTCACCCGTGGACGCACTGTCACTTGCCGCTGCCC delta-geobacter_metalli 335 --GGGCC-GACATCCGGATCGTCTACTCCCCCTTGGACGCCGTTGCCATTGCTGCCAGGA delta-Pelobacter_propion 335 --GCGCG-GATGTCAGGATTGTCTACTCGCCGCTGGACGCTGTGGCCATCGCCAGGAAGA delta-geobacterFRC32 335 --GTGCG-GATATCAGGATCGTCAACTCGCCTCTGGATGGGGTTGCACTGGCGGCACGGA proteo-others-Mariprofundus_fe 335 --GTGCC-GATATCCGCATGATCTACTCGGCCACCGATGTGCTCGCCATCGCACAGGCCA alpha-Stappia_agg 335 --GCGCC-GATATCCGGATGGTCTATTCGACCCAGGATGCTCTGCGGATCGCCCGGGAGA beta-Methylibium_petro 335 --GTGGC-GACATCCGCATGGTGTACTCGGCGGCCGATGCGCTCCAGATCGCGCGCCAGA beta-Ralstonia_metall 335 --GTGCG-GACATCCGCATGGTCTACTCGGCGGCCGACGCGCTGGCCATCGCCCAGCAGA beta-Rhodoferax_ferri 335 --GTGGC-GACATTCGCATGGTCTATTCCACGCTCGATGCCTTGCAGATTGCGCGCGACA beta-dechloromonas_aro 335 --GCGGC-GACATCCGCATGGTTTATTCGAGCGCCGATGCCGTGACCTTGGCGCAAAAGA beta-Azoarcus 345 CCGCGCC-GACATCCGCATGGTGTATTCCGCCGCCGATGCGCTCGCGCTCGCCCAGCGCG beta-Burkholderia-viet 335 --GCGCC-GACATCCGGATGGTTTATTCGCCGCTCGACGCGCTGAAGATCGCGCGCCTGA alpha-Magnetospirillum_mag 335 --GCGCC-GACGTGCGCATGGTCTATTCCACCCTGGACGCCATCAAGCTGGCCCAAGCCA alpha-Bradyrhizobium_jap 344 --GTGCC-CACATTCGGATGGTCTACTCGACCCTCGACGCTATCCGGCTCGCCGAACAGA alpha-BradyrhizobiumBTAi1 335 --GCGCC-GACATCCGCATGGTCTATTCGACCTTGGACGCGATCGCGCTCGCCGAGCAGA alpha-Rhodopseudomonas_palBisA 335 --GGTGC-GATATCCGCATGGTGTATTCGACGCTGGATGCGCTCGCGATCGCCGAGGCCG alpha-Rhodopseudomonas_palCGA0 335 --GCGCC-GACATCCGCATGGTGTATTCGACGCTGGATGCGATCGCGATCGCCGAGGTGG beta-Burkholderia_phy 335 --GCGCC-GACGTGCGCGTCGTCTATTCGAGCGCCGACGCGTTGGCATTCTCTCGCGCGC alpha-Rhodospirillum_rub11170 344 --GCGCC-GATATCCGCATGGTCACCTCGCCGTTGCAAGCGGTGACCATCGCCAGGGACA beta-Burkholderia_xeno 356 --GCGCT-GACGTGCGGATGGTCTATTCTCCCGTCGACGCGCTGGCGATTGCACGTGCGA alpha-Sphingopyxis_alask 347 --GGGCC-GATGTGCGGATGGTCTATTCACCACTCGATGCCCTCGCGCTGGCCCGTGCGA gamma-Shigella_flex 347 --GTGCC-GATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGA gamma-Shigella_sonn 347 --GTGCC-GATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGA gamma-Shigela_dys 347 --GTGCC-GATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGA gamma-EcoliK12 347 --GTGCC-GATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGA gamma-Ecoli_CFT073 347 --GTGCC-GATGTGCGCATCGTTTACTCGCCAATGGATGCGTTGAAACTGGCGCAGGAGA gamma-salmonella_ent 347 --GCGCG-GATGTGCGTATTGTTTATTCTCCGATGGACGCGCTGAAGCTGGCGCAGGATA gamma-salmonella_LT2 347 -----CG-GATGTGCGTATTGTTTATTCTCCGATGGACGCGCTGAAGCTGGCGCAGGATA gamma-enterobacter_638 347 --GTGCG-GATGTGCGAATCGTCTATTCACCCGTGGATGCGCTGAAGCTGGCGGCTGAAA gamma-erwinia_caro 347 --GAGCA-GACATCCGCGTGGTTTACTCCCCTCTGGATGCACTAAAACTGGCTCGCGATA gamma-Yersinia_ber 347 --GCGCG-GATATTCGCGTGGTCTATTCACCGTTGGATGCACTGACGCTGGCACAGCATA gamma-Yersinia_int 347 --GCGCG-GATATTCGCGTGGTCTATTCACCGTTGGATGCACTGACGCTGGCACAGCATA gamma-Yersinia_fred 347 --GAGCA-GATATTAGAGTGGTTTACTCACCACTGGATGCCCTGACGCTGGCACAGAATA gamma-Yersinia_moll 347 --GCGCG-GATATTCGCGTGGTCTACTCGCCACTGGATGCCCTGACACTGGCACAGCGCA gamma-Serratia_protea 347 --GCGCC-GATGTGCGGGTGGTTTATTCGCCGTTGGATGCTCTGATGCTGGCGCGTCAAC alpha-Rhodobacter_sphaer 347 --GCGCG-GACATCCGCATGGTCTATTCGCCGCTCGACGCGCTCGAGATCGCGCGGCGCA alpha-Rhodobacter_spaer17025 347 --GGGCC-GACATCCGCATGGTCTATTCGCCGCTCGACGCGCTGGAACTCGCGCGGCGCA alpha-Roseovarius-2601 347 --GCTGC-GACATCCGCATGGTCTATTCGCCGCTCGACGCGCTCGAGCTGGCGCGGCGCA alpha-Paracoccus_deni 347 --GGGCG-GATATCCGCATGGTCTATTCGCCGCTGGATGCCCTGGCTCTGGCCCGGAAAT alpha-Xanhobacter_auto 347 --GGGCG-GACGTGCGCATGGTCTATTCGCCCCTTGATGCGCTCACCTTGGCGAAGCGCA actinobacteria-corynebacterium 332 --GCGCC-GACGTCCGCTTCGTGTACTCGCCTCTCGACGCCCTTAAAGTAGCCAAGGACA Actinobacteria-RhodococcusRHA1 329 --GCGCG-GACGTCCGCTTCGTGTACTCGCCGCTGGACGCGCTGAAGGTGGCACTCGACA Chloroflexi-Acidobacteria 326 --GCGGC-GATGTGCGCATCGTGTACTCGCCGATGAACGCACTAGAGCTTGCGAAAGCGA Chloroflexi-Solibacter_usi 326 --GCGGC-AGCGTGCGCATTGTCTACTCGCCAATGGACTGCTTGAACATCGCGCAGCAGA delta-proteo_MMS1 419 --GGGCC-GATGTACGGATGGTCTACTCGCCGCTGGAGGCGGTGGAACTGGCCCGGCAGC Cyan-SynechococcusWH5701 329 --GAGCC-GACGTGCGCGTGGTGACCGCACCCCTCGACGTGCTGGCTCTGGCGAGGTCTC Cyan-Synechocystis6803 326 --GCGGC-GATGTGCGCATTGTCTATTCTCCTTTGGATTGTTTGGCGATCGCCAGGGAGA chloroflexi-chloroflexus_agg 326 --GTGGT-GACGTGCGCGTCGTCTATTCCCCGCTCGACGCAGTAAAGCTGGCCCAACAAC chloroflexi-chloroflexus_aur 326 --GTGGC-GATGTGCGTGTTGTCTACTCGCCGCTCGATGCCGTCAAACTGGCCCAACAAA Chloroflexi-Roseiflexus_cas 326 --GCGGT-GATGTGCGTGTGGTGTATTCACCGCTCGATGCCGTGCGCCTGGCGCAGCAGC Chloroflexi-RoseiflexusRS-1 326 --GCGGC-GATGTGCGGATCGTCTACTCCCCGCTCGATGCTGTGCGCCTGGCGCAGCAAC Actinobacteria-Mycobacterium_g 326 --GCGCG-GACGTGCGCGTCGTCTACTCACCCCTGGACGCGCTGACGATCGCCCGGGACA Actinobacteria-Mycobacterium_v 326 --GCGGC-GACGTGCGCGTCGTCTACTCCCCGCTGGACGCGCTGACGATCGCGCGGGAGC Actinobacteria-FrankiaCcI3 326 --GCGCC-GACGTCCGGGTGGTCTACTCCCCGCTGGACGCCCTGACGATCGCCCAGCGCA Actinobacteria-FrankiaACN14a 326 --GCGCC-GACGTGCGGGTGGTGTACTCGCCGCTGGACGCGTTGACGATCGCCCGGCGCA Actinobacteria-Streptomyces_av 326 --GCGGC-GATGTACGCGTCGTCTACTCGCCGCTCGACGCCCTGCGGATCGCGCGGCAGA Actinobacteria-Mycobacterium_s 326 --GCGGT-GACGTGCGCATCGTCTACTCACCGCTGGACGCCACCCGTGTGGCCGCCGACA Chlorobi-Pelodictyon_lut 326 --GGGGT-GACGTCCGCGTTGTCTTCTCTCCGCTTGACGCACTCCAGATTGCCCGAGACA

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Chlorobi-Pelodicton_lut 326 --GGGGC-GATGTGCGCATTCTCTTTTCCCCGCTTGATGCCCTCCAGATTGCTCGTGACA chlorobi-chlorobium_lim 326 --GCGGG-GATGTAAGAATCGTCTATTCTCCGCTCGAAGCTCTGCAGTTCGCGCGCGATA Chlorobi-Prosthecochloris_aest 326 --GCGCC-GATGTGCGTTTTGTTTTTTCGCCCCTTGAGGCGCTTCAGATTGCGCGAGATA chlorobi-chlorobium_tep 326 --GAGCG-GATGTGCGGGTCGTTTTTTCGCCGCTCGAAGCGTTGCAAATCGCGCGCGACA delta-Syntrophus_acid 338 --GGGCG-GATGTGCGCATTGTCTCTTCGACCCTGGACAGCCTTCGCCTCGCTGAAGAGA delta-desulfotalea_psy 326 --GGGCC-AAGGTAGACATTGTCTACTCACCCATGGACGCCCTGGAAATTGCCAAGAACA chloroflexi-dehalococcoides_et 326 --GGGCG-GACGTACGTATTGTCTACTCTACCATGGACGCACTGGAGATAGCCCGCCAGA chloroflexi-dehalococcoides_CB 326 --GGGCA-GACGTACGTATTGTTTACTCTACCATGGACGCACTGGAGATAGCCCGCCAAA clostridia-desulfitobacterium_ 335 --GGGCG-GATATCCGCATTGTTTACTCCACACTGGACGCCTTGGACCTGGCCCGGAAGA Clostr-Moorella_therm 347 --GTGCC-GACGTGCGCATTTTTTACTCGCCCCTGGACGCAGTGGCCTTTGCTGAAGCCA Bacteroides-Robiginitalea_bif 326 --GAGCA-GATGTACGCATTTTATATTCTCCAATGGAGGCCGTCCAAATCGCAAGTGAAA epsilon-Helicobactr_acin 344 --GGCTG-GATGCACGCTTTTTGTATTCGCCCATGCAAGCTTTAGAAATCGCTAAAGAAA epsilon-Helicobacter_pylor 344 --GGTTG-GATGCACGCTTTTTGTATTCGCCCATGCAAGCTTTAGAGATCGCTAAAGAAA gamma-PhotobacteriumSKA34 350 --GCTGT-GATATTCGACCGATCTACGATCCGCTTGATTGCTTAGCTATTGCGACAGAAA gamma-Vibrio_ang 350 --GCTGT-GATATTCGACCTATCTATGATCCGCTTGATTGCTTAACTATTGCGACAGAAA Gamma-Photobacterium_prof 347 --GCGGT-AATATCGAAGCCATTTATGATCCGATGGATGTGCTAAAAATCGCCCGTAACA epsilon-Wolinella_succ 344 --GGCGT-GATGTTCGATTCGTCTATTCACCCCTAGAGGTGCTCAAAATTGCCAAAGAGA epsilon-campylobacer_con 326 --GGCAC-GACATAAGGGCACTTTACACGCCGCTTGATGCGCTAAATATAGCTAAGCAAA Aquifex 353 --GTGCG-GACGTCAGGATGGTTTACTCGTGCTTAGATACGCTAAAAATAGCAAAAGAAA gamma-Actinobacillus 350 --GTGCC-GATATTCGTATCGTCTATTCTCCCTTGGATGCACTCAATATTGCCCGACAAA gamma-Manheimia-succ 350 --GTTGT-GACGTACGCATTGTTTATTCTCCGCTGGATGCGTTAAATATCGCCTTAAGCA gamma-Acidobacillus_succinogen 350 --GAGCG-GATGTTCGCATTGTTTATTCGCCGTTGGACGCTTTAACTCTGGCGCGCAATA Cyano-Avar 335 --GCGCA-GATATCCGCATGGTTTACTCTCCCCTAGATAGCCTGCAAATTGCGAGAAATC Cyan-Nostoc7120 335 --GCGCA-GATATCCGCATGGTTTACTCTCCCCTAGATAGTCTGCAAATTGCCAGAAATC Cyan-Nodularia_spu 335 --GCGCA-GATATCCGCATGGTATACTCCCCATTAGATAGCCTGCAAATCGCCAGAGACA Cyan-Lyngbya8106 347 --GTGCA-GATATTCGCATGGTTTATTCACCCTTGGATGCCTTACCCATTGCCAAACAAA Cano-trichodesmium_ery 347 --GTGCA-GATATTAGAATGGTTTATTCGCCCCTGGATAGTTTGAAGATAGCTAAAGAAA clostridia-caldicelluosiruptor 299 --GTGGAAAA-ATAAAGGTTATGTACTCACCTGTGGATGCAGTGGAACATCTTGAAAATA clostridia-clostridium_beij 307 --GGAAAAGATATTAAAGTATTATATTCACCTTTAGATTCAATAGCACTTGCAGAAGAAA Methanobacteriales-Methanospha 296 --GTGCA-GATGTAAGAATAGTATATGGTATTGGCAATGCAGTAGAATTAGCAAAAGAAA Thermococcales-Pyrococcus_abys 296 --GTGCA-GATGTAAGAATAGTATATGGTATTGGCAATGCAGTAGAATTAGCAAAAGAAA Methanococcales-Methanocaldoco 320 --GTTGT-GATGTTAGAATTGTCTATAGTATAAGTGAAGCAGTAAAGATGGCTAAGAAGG Methanococcales-Methanococcus_ 332 --GAGCA-GATGTAAAAATCGTTTACGGAATTGGAGATGCTGTAAAACTTGCAAAAAAAC delta-Lawsonia_intra 329 --GTGCAAGG-ATTTCTATTATCTATTCTCCTATGGATATTTTAAAAATTGCTATTGATA epsilon-campylobacter_jej 326 --GGGCT-GATGTTAGAGCACTTTATTCTCCGCTTGAAGTTTTGGAAATCGCTAAGCAAA epsilon-campylobacter_ups 326 --GTGCT-GATGTAAGAGCGCTTTATACTCCACTTGAAATTTTGCAAATCGCTAAGCAAA clostridia-clostridium_therm 299 --GGGGA-AATGTAAAAATCCTCTATTCTCCCCTCAGTGCGGTAAAAATTGCAATCAAAA clostridia-carboxidothermus_hy 320 --GCTTT-TCTGTAAAAGTTGCTTATTCTCCCCGGAATGCAATTGAATTTGCCGAAAAAA Clostr-Halothermothrix_oren 329 --GAAAA-AATATAACTACAGTTTATTCTCCCCTTGAAGCTGTAAAGATTGCATATAATA Methanosarcinales-Methanosarci 329 --GAACA-GACGTAAGGATGGTCTACAGCATTGATGATGCCGTAGCCCTCGCAAACAAAA Methanosarcinales-Methanosarci 329 --GGGCA-AATGTAAAGATGGTCTATAGCATTGACGATGCAATTGCCCTTGCAGAAAAAA Clostridia-Thermoanaerobacter_ 311 --GGAAA-GAGGTAAGAGTGTTTTACTCTCCTTTGGACGCTTTGAAGTACGCAGAGGAGA clostridia-clostridium_cell 329 --GCAGC-AACGTCCAGATGGTGTATTCTCCACTTAATGCAGTTGAATTGGCGAGAAATA cyano-crocosphaera_wat 326 --GTGGG-GATGTTCGTATTGTTTACTCACCTCTAGATAGTTTAAAAATTGCTCAAGAAA Bacteroides_Flavobacteriu_john 323 --GCGGT-GATTTACGCATTTTATATTCTCCTTTAGAAGCTGTAAATATTGCGGCTAAAA

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Proteo-others-MagentococcusMC1 380 ACCCCACCCGTGAAGTGGTCTTTTTTGCCAGTGGTTTTGAGACGGTCTCGGCACCACTG beta-Nitrospira_multi 377 ACGCGAAACGACAAGTCGTTTTCTTTGCTGCGGGTTTTGAGACCACCACAGCCCCGGTA beta-Thiobacillus_denitri 374 ACCCGACGCGGCCCGTGGTGTTCTTCGCCGCGGGCTTCGAAACGACGACCGCGCCGACC alpha-Acidiphilum 383 CGCCGAGCCGCCCGGTCGTCTTCTTCGTCGCCGGCTTCGAGACGACGACGGCGCCGGTC Thermococcales-Pyrococcus_fur 398 ATCCAGATAAGCTTGTAGTGCACTTTTCTCCTGGGTTTGAGACTACCGCCGCTCCAACA Thermococcales-Pyrococcus_hori 398 ATCCAGATAAGCTTGTCGTTCACTTCTCTCCTGGGTTTGAAACTACAACTGCTCCGACA Thermococcales-Thermococcus_ko 410 ACCCGGACAAAACCGTCGTCCACTTCTCGCCGGGCTTTGAGACGACAACCGCTCCAGCG Archaeoglobales-Archaeoglobus 407 GCTCGGATAGGCCAGTTGTTCACTTCGCCATCGGCTTTGAAACGACCATGCCCTCAACA Methanopyrales-Methanopyrus_ka 359 CCGAGGACCGTGATGTCCTCTACCTGGCGGTAGGGTTCGAGACTACGGCACCGACCACC Methanomicrobiales-Methanospir 341 ATACCGATAAAGAAGTGGTTTTCATTTCGGTCGGATTTGAAACAACCGCACCAACCGTT Sulfolobales-Metallosphaera_se 401 AACCAAACAAGGATTTCCTTTTCTTCGCAGTGGGAATGGACACTACGGCCCCGGCCACT Thermoproteales-Pyrobaculum_is 437 AC---AACAAGCCCTCCCTCTTCCTGGGCATTGGGTTTGAGACAGTGGCGCCGGGCTAC Methanobacteriales-Methanother 350 AACTTGACCGGGAAGTCGTATTCATGGCAGCGGGATTTGAGACCACGGCGCCAACAACA Methanomicrobiales-Methanocull 344 AACCGGACCGGGAGGTCGTCTTCATATCGGTCGGGTTCGAGACGACCGCACCGACGGTC Thermoproteales-Thermofilum_pe 461 ATGGGGGC---GAGTCGGTCTTTCTCGGTATAGGCTTCGAGACCGTTGCCCCCGGCTAC delta-Anaeromyxobacter 404 ACCCCGAGGAGCAGGTGGTGTTCCTGGCGAGCGGCTTCGAGACCACCGCGGTCGCGACC delta-desulfovibrio_des 386 ACCCGCAGTCCACGGTGGTTTTTCTGGGCATCGGTTTTGAAACCACCACCCCCGCCGTG delta-geobacter_metalli 392 ATCCGGAGAAGCGGGTGGTCTTTCTCGGCGTCGGCTTCGAGACCACGGCCCCGGCCATT delta-Pelobacter_propion 392 ATCCCTGTAAAAGGGTCGTCTTTCTGGGGGTCGGCTTCGAGACAACGGCGCCCGCCATT delta-geobacterFRC32 392 ACCCGGACAGAAAGATCATTTTTCCAGGCATCGGATTCGAGGCCACGGCACCGGCCGTT proteo-others-Mariprofundus_fe 392 ACCCCGAACGTCAGGTGGTCTTTTTTGCCATCGGTTTTGAAACCACCACGCCGCCGACT alpha-Stappia_agg 392 ACCCGCAGCGGCAGGTGGTGTTCTTCGCCATCGGCTTCGAGACGACCACGCCGCCAACG beta-Methylibium_petro 392 ACCCGCAGCGCGAGGTGGTGTTCTTCGCGATCGGCTTCGAGACGACCACGCCGCCCACG beta-Ralstonia_metall 392 ATCCCCAGCGCGAAGTGGTGTTTTTCGCCATCGGCTTCGAGACCACCACGCCGCCCACC beta-Rhodoferax_ferri 392 ACCCCGAGCGCGAGGTGGTGTTTTTTGCCATCGGGTTTGAGACCACCACACCGCCAACG beta-dechloromonas_aro 392 ATCCGGACAAGCAGGTCGTTTTCTTCGCCATCGGTTTCGAAACGACGACGCCGCCGACG beta-Azoarcus 404 AGCCGCAGCGCGAGGTGGTGTTCCTCGCGATCGGCTTCGAGACCACCACGCCGCCCACC beta-Burkholderia-viet 392 ATCCCGATCGCGAGGTCGTGTTCTTCGCGATCGGCTTCGAGACCACCACGCCGCCCACC alpha-Magnetospirillum_mag 392 ACCCCGACCGCCAGGTGGTGTTCTTCGCCATCGGCTTCGAGACCACCACGCCGCCCACC alpha-Bradyrhizobium_jap 401 CGCCGGGCCGCGAAGTGGTCTTCTTTGCCATCGGATTTGAGACCACGACGCCCCCGACG alpha-BradyrhizobiumBTAi1 392 ATCCCAGCCGTGAGGTCGTGTTCTTCGCCATCGGCTTCGAGACCACGACGCCGCCCACC alpha-Rhodopseudomonas_palBisA 392 AGCCGAGCCGGGAGGTGGTGTTTCTCGCTATCGGCTTCGAGACCACCACGCCGCCGACC alpha-Rhodopseudomonas_palCGA0 392 AGCCGAACCGCGAGGTGGTGTTCTTCGCCATCGGTTTCGAAACCACGACGCCGCCGACC beta-Burkholderia_phy 392 GATCTGACCGACAGGTTGTGTTTTTCAGCATTGGCTTCGAGACGACCACGCCCCCCACT alpha-Rhodospirillum_rub11170 401 ATCCCCAACGCGCCGTGGTGTTCTTCGCCATCGGCTTTGAAACCACCGCCCCGGCCTCG beta-Burkholderia_xeno 413 ATCCGCATCGCGAAGTGATCTTCTTCGGGCTCGGCTTTGAAACCACGATGCCGGGCACG alpha-Sphingopyxis_alask 404 ACCCGGACCGCGAAGTGGTTTTCTTCGGCCTCGGCTTCGAGACGACGATGCCCTCCACA gamma-Shigella_flex 404 ATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACCATGCCGACCACC gamma-Shigella_sonn 404 ATCCAACCCGTAAAGTCGTATTCTTCGGCTTAGGTTTTGAAACCACCATGCCGACCACC gamma-Shigela_dys 404 ATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACCATGCCGACCACC gamma-EcoliK12 404 ATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACCACC gamma-Ecoli_CFT073 404 ATCCAACCCGTAAAGTGGTATTCTTCGGCTTAGGTTTTGAAACCACCATGCCGACCACC gamma-salmonella_ent 404 ACCCGACCCGCAAAGTGGTATTTTTCGGGCTGGGCTTTGAAACCACCATGCCGACCACC gamma-salmonella_LT2 401 ACCCGACCCGCAAAGTGGTATTTTTCGGGCTGGGCTTTGAAACCACCATGCCGACCACC gamma-enterobacter_638 404 ATCCAACGCGCAAAGTGGTGTTTTTCGGCCTGGGGTTTGAAACCACCATGCCCGCCACA gamma-erwinia_caro 404 ATCCGCAGCGCAAAGTGGTATTTTTCGCCCTGGGGTTTGAAACCACCATGCCAGCAACC gamma-Yersinia_ber 404 ATCCTGATCGCCAGGTGGTGTTTTTTGGCCTCGGTTTTGAAACCACCATGCCTGCCAGT gamma-Yersinia_int 404 ATCCTGATCGCCAGGTGGTGTTTTTTGGCCTCGGTTTTGAAACCACCATGCCTGCCAGT gamma-Yersinia_fred 404 ACCCTTCCCGCGAGGTGGTATTTTTCGGACTGGGTTTTGAAACGACCATGCCCGCCAGT gamma-Yersinia_moll 404 ACCCCACGCGCGAGGTGGTGTTTTTCGGCCTCGGCTTCGAAACCACTATGCCCGCCAGT gamma-Serratia_protea 404 ACCCGCAGCGGCAGCTGGTGTTCTTTGCCCTGGGGTTTGAAACCACCATGCCGGTCACG alpha-Rhodobacter_sphaer 404 ATCCTGCGCGCGAGGTGGTCTTCTTCGGCCTCGGCTTCGAGACGACGACGCCCTCGACC alpha-Rhodobacter_spaer17025 404 ATCCGGGGCGCGAGGTGGTCTTCTTCGGCCTCGGCTTCGAGACCACGACACCGTCCACG alpha-Roseovarius-2601 404 ACCCCGACCGCGAGGTGGTGTTCTTCGGTCTCGGCTTCGAGACCACGACCCCCTCGACC alpha-Paracoccus_deni 404 ACCCCGAACGCGACGTGGTGTTCTTTGGTCTGGGCTTCGAGACGACGACCCCTTCGACC alpha-Xanhobacter_auto 404 ATCCCGAGCGGCAGGTGGTGTTCTTCGGCCTCGGCTTCGAGACCACCATGCCCTCCACC actinobacteria-corynebacterium 389 ATCCCACCAAAATGGTGGTGTTTTTCGCCGTAGGTTTTGAAACGACTGCCCCGTCGACT Actinobacteria-RhodococcusRHA1 386 ATCCCGACCACCAGGTGGTGTTCTTCGCCGTCGGGTTCGAGACGACGGCGCCGTCCACC Chloroflexi-Acidobacteria 383 ACCCAACGAAGCAGGTTGTCTTCTTCGCGGTTGGCTTCGAAACCACCGCGCCCGCCAAT Chloroflexi-Solibacter_usi 383 ACCCGGAAAAGCAGGTCGTGTTCTTCGCGATCGGATTCGAGACTACGGCGCCGGCGAAT delta-proteo_MMS1 476 AGCCCCACCGGCAGGTGGTTTTCTTCGCCGTGGGTTTTGAAACCACCGCTCCCGGCAAC Cyan-SynechococcusWH5701 386 ATCCCGAGCGGCAGGTGGTGTTCCTCGCGGTGGGCTTCGAAACCACCGCCCCGGCCACC Cyan-Synechocystis6803 383 ATCCTAATCGGGAAGTGGTATTTTTCGGAGTAGGTTTTGAAACTACAGCCCCTGCCACG chloroflexi-chloroflexus_agg 383 ACCCCGACCGCCAAGTCGTCTTTTTTGCCATCGGCTTCGAGACTACTGCACCCGCCAAC chloroflexi-chloroflexus_aur 383 ACCCCGACCGTGAAGTCGTCTTTTTTGCCATCGGCTTCGAGACAACTGCCCCCGCCAAT Chloroflexi-Roseiflexus_cas 383 ACCCCGACCGTGAGGTGGTCTTCTTTGGCATTGGTTTCGAGACGACTGCTCCCGCCAAC Chloroflexi-RoseiflexusRS-1 383 ACCCCGACCGCGAGGTTGTCTTCTTCGGCATCGGGTTCGAGACGACCGCGCCTGCCAAT Actinobacteria-Mycobacterium_g 383 ACCCGGACAGACAGGTCGTGTTCTTCGGCATCGGGTTCGAGACCACTGCCCCGGCGAAC Actinobacteria-Mycobacterium_v 383 ATCCGGACAAACAGGTGGTGTTCTTCGGCATCGGTTTCGAGACCACCGCCCCCGCCAAC Actinobacteria-FrankiaCcI3 383 ACCCCGATCGGGAGGTCGTGTTCTTCGGGATCGGATTCGAGACCACCGCGCCGGCCAAC Actinobacteria-FrankiaACN14a 383 ACCCCGACCGGCAGGTGGTGTTCTTCGGGATCGGCTTCGAGACGACGGCACCGGCCAAC Actinobacteria-Streptomyces_av 383 ACCCGGACCGCGAGGTGGTGTTCTTCGGCATCGGCTTCGAGACGACGGCACCCCCCAAC Actinobacteria-Mycobacterium_s 383 ACCCGGACAAGCAGGTGGTGTTCTTCGGGGTCGGCTTCGAGACCACCGCACCCGCCAAC Chlorobi-Pelodictyon_lut 383 ATCCTTCTCGGGAGGTTGTTTTTTTTGCTGTAGGGTTTGAAACCACCGCACCTGCCAAT ____________________ Position of primer hypD-rev

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Chlorobi-Pelodicton_lut 383 ATCCCTCAAAAGAGGTGGTCTTCTTTGCCGTTGGATTTGAAACTACTGCGCCAGCCAAC chlorobi-chlorobium_lim 383 ATCCGCAGAAGGAGGTTGTATTTCTTGCCGTGGGCTTCGAGACTACTGCACCGGCCAAT Chlorobi-Prosthecochloris_aest 383 ATCCCCGGCATGAAGTGGTTTTTCTTGCGGTCGGCTTTGAAACGACAGCTCCGGGCAAT chlorobi-chlorobium_tep 383 ATCCGTCGAAAGAGGTGGTGTTTCTCGGCGTCGGTTTTGAGACCACGGCTCCGGCCAAC delta-Syntrophus_acid 395 ACCCGGAAAAGGAGGTTGTTTTCATGGGAATCGGCTTCGAAACAACGGCGCCGACCGTG delta-desulfotalea_psy 383 ATCCAGACGAACTGGTGGTCTTTCTCGGCGTTGGTTTTGAAACAACCACCCCGACAATT chloroflexi-dehalococcoides_et 383 ACCCCTTGAAAAAGGTTATCTTTGTGGGTATAGGTTTTGAAACCACCGCCCCCACCATT chloroflexi-dehalococcoides_CB 383 ACCCTGCCAAAAAGGTTATCTTTGTGGGTATAGGCTTTGAAACCACCGCCCCCACCATT clostridia-desulfitobacterium_ 392 ACCCTGACAAAGAAGTGGTCTTTCTCGGCGTGGGCTTTGAGACCACCGTTCCCACGGTG Clostr-Moorella_therm 404 ACCCGGAAAGAGAGGTCGTGTTTTTAGGGATTGGTTTTGAAACGACTGCCCCGGCAGTC Bacteroides-Robiginitalea_bif 383 ACCCTGACAAAGAAGTGGTCTTTTTTGCGGTGGGCTTTGAGACTACCGCTCCCGCAAAT epsilon-Helicobactr_acin 401 ACCCGCATAAAAAAGTCATTTATTTTGCGATCGGTTTTGAAACCACCACACCCATGACG epsilon-Helicobacter_pylor 401 ACCCTACTAAAAAAGTCATTTATATTGCGATCGGTTTTGAAACCACAACGCCCATGAGC gamma-PhotobacteriumSKA34 407 ACCCAGATAAAGAGGTGGTTTTCTTCGCCATTGGTTTTGAAACCTCAACACCTATGACC gamma-Vibrio_ang 407 ACCCAGATAAAGAGGTGGTTTTCTTTGCTATTGGTTTTGAAACCTCAACGCCTATGACC Gamma-Photobacterium_prof 404 ATCCAGATAAACAGGTGGTCTATTTTGCTATTGGGTTTGAAACGACAACCCCGATGACA epsilon-Wolinella_succ 401 ATCCCCACCAAAAAGTGGTCTTTTTCGCCATTGGATTTGAGACCACCACGCCTATGAGT epsilon-campylobacer_con 383 ATCCAGACAAAAAGGTCATATTTTTTGCGATCGGCTTTGAGACGACGACTCCGATGAGC Aquifex 410 ATCCTGATAAAAAGGTTATCTTCTTTGCCATAGGTTTTGAAACCACTACTCCCCAAACC gamma-Actinobacillus 407 ATCCGAACAAAAAAGTGGTTTTCTTTTCTCTCGGATTTGAAACGACAATGCCAAGTACG gamma-Manheimia-succ 407 ATCCCGATAAAAAAGTGGTATTTTTTTCGCTTGGTTTTGAGACTACAATGCCCGCCGCC gamma-Acidobacillus_succinogen 407 ATCCGGATAAACAAGTGGTGTTTTTCTCTTTGGGCTTTGAAACCACCATGCCGAGTGCG Cyano-Avar 392 ACCCTGATAAAGAAATTGTATTCTTCGCTTTAGGCTTTGAAACCACAGCCCCCAGCACC Cyan-Nostoc7120 392 ACCCTGATAAAGAAATTGTATTCTTCGCTTTGGGCTTTGAAACCACAGCCCCCAGCACC Cyan-Nodularia_spu 392 ACCCAGACAAAGAAATAGTCTTCTTCGCCCTCGGCTTTGAAACCACCGCCCCTAGTACC Cyan-Lyngbya8106 404 ACCCAGATAAAGAAGTAATTTTCTTTGGTTTGGGCTTTGAAACTACAGCCCCAAGTACC Cano-trichodesmium_ery 404 ACCCAGATAAAGAGATAATTTTCTTTGGTTTAGGTTTTGAAACTACTGCCCCCAGTGCA clostridia-caldicelluosiruptor 356 ACGAAAA------AGTTATTTTTGCTGCAGTCGGGTTTGAGACAACAGCACCTGCATTT clostridia-clostridium_beij 365 ATAAAGATAAGGAAATAGTTTTTCTTGGAGTAGGATTTGAAACTACAACTCCTATAATC Methanobacteriales-Methanospha 353 TTGATAAT---GATGTTGTATTCATGGCAGCAGGATTTGAAACAACAGCACCAACAACA Thermococcales-Pyrococcus_abys 353 TTGATAAT---GATGTTGTATTCATGGCAGCAGGATTTGAAACAACAGCACCAACAACA Methanococcales-Methanocaldoco 377 AGAGAGATAAGAAGTTTGTTTTTGTGGCAATAGGTTTTGAAACCACTGCTCCAACTACT Methanococcales-Methanococcus_ 389 AG---GATGAAAAGGTAGTATTTGTTGCAATCGGATTTGAAACTACTGCTCCAACAACT delta-Lawsonia_intra 386 ATCCAAAAGATATAGTAATTTTACCAGGGATTGGTTTTGAAACAACAGCTCCTACTATA epsilon-campylobacter_jej 383 ATTTAAACAAAAATATCATCTTTTTTGCTATAGGTTTTGAAACCACTACGCCTATGAGT epsilon-campylobacter_ups 383 ATTTAGATAAAAAAATCATTTTTTTCGCCATAGGTTTTGAAACAACAACGCCTATGAGT clostridia-clostridium_therm 356 GCCCTGAGACCCAATTTATTTTTGCCGCAGTGGGGTTTGAGACCACAGCCCCTATTTAT clostridia-carboxidothermus_hy 377 ATCCCAATATTGAAGTGGTATTTTTTGGAGCCGGGTTTGAAACCACTGCGCCGGCTTTA Clostr-Halothermothrix_oren 386 ACCCCGGGCAGGAGGTTATTTTTCTGGCTATTGGTTTTGAAACTACCATACCTGCTATT Methanosarcinales-Methanosarci 386 AACCTGAGCTCGAAGTAGTCTTTTTCGGGATCGGCTTTGAGACTACAGTCCCTGCCAAC Methanosarcinales-Methanosarci 386 AGCCAGAACTTGAGGTAGTCTTTTTTGGAATAGGCTTTGAGACCACGGTTCCTGCAAAT Clostridia-Thermoanaerobacter_ 368 ATCCGCAAAAGGAAGTTGTCTTCATAGGTATAGGCTTTGAGACCACTGTCCCTACAGTG clostridia-clostridium_cell 386 ATCCAGACAAGGAGGTTGTTTTTTTATCGGTAGGTTTTGAAACAACAACACCTGTAATA cyano-crocosphaera_wat 383 ATCCTGATAAAGAGGTTGTTTTTTTTGCTGTTGGCTTTGAAACAACCACCCCAGCAACA Bacteroides_Flavobacteriu_john 380 ATCTGGATAAGGAAATTGTATTTTTTGCTGTTGGTTTTGAAACTACTGCGCCCAGCAAT ____________________ Position of primer hypD-rev

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Proteo-others-MagentococcusMC1 439 GCGGCGCAGTTTC------------TGGCGGGTGTGCCGGATAATTTCTCGATTTTAAAT beta-Nitrospira_multi 436 GCAGCCATGCTGC------------TGGAGGGGGTACCGGACAATTTATCCATCCTGTTG beta-Thiobacillus_denitri 433 GCCGCCATGCTGG------------CCGAAGGCGTTCCCGACAATCTGAGCATTCTGCTG alpha-Acidiphilum 442 GCCGCCATGCTGG------------CCGAGGGCGTGCCGGACAATCTCTCGGTCCTGCTC Thermococcales-Pyrococcus_fur 457 GCTGGAATGCTTGAGAG-CAT--TGTGGAAGAGGGGCTAGAGAACTTTAAGATTTATTCC Thermococcales-Pyrococcus_hori 457 GCAGGGATGCTTGAAGT-TGT--TGTTGAGGAGGGTTTAGAAAACTTCAGGATTTATTCT Thermococcales-Thermococcus_ko 469 GCCGGAATGCTCAACGT-CGC--CGCCCAAGAGGAGCTTGAGAACTTCAAGATATACTCC Archaeoglobales-Archaeoglobus 466 GCTATAGCCCTTC-------T--TGATGAGAAGG------AGAACTTCTACGTCTTCTCA Methanopyrales-Methanopyrus_ka 418 GCGGCCGTGCTCC------------TCGATGACCCGCCCTCCAACTTCTACGTGCTGTCG Methanomicrobiales-Methanospir 400 GCAGCAATGCTTC------------TGACAAATCCTCCGAAGAATTTCTCCATATTGAGT Sulfolobales-Metallosphaera_se 460 GCCTTTGAAATGA-------T--GAAGGG---TGTTCCGGACAACCTGAGCTTCCTTGTT Thermoproteales-Pyrobaculum_is 493 TCCGAGGCCATCT-------T--GAGGGGGCTTGTGCCTCCTAACCTCAAGCTGATGTCT Methanobacteriales-Methanother 409 GCCTCGGAGATAC------------TCTCAGGACCACCTGAGAACTTCTCGGTTCTTTCA Methanomicrobiales-Methanocull 403 GCCGCCACGATCC-------T--CTCCCG---CCCGCCGGAGAACTTCTCGATCCTCTCG Thermoproteales-Thermofilum_pe 517 GCCCGCGCGGTCG-------T--CTCGGGGAGCCTCCCGGAGAACCTCAAGCTCATGAGC delta-Anaeromyxobacter 463 GCGGCGGTGGCGC------------TCGCCTCCCCGCCCCCGAACTTCTCCATCCTCTCG delta-desulfovibrio_des 445 GCCGCCACCATCAAAAC-CGC--AGACCGGCAGGGGCTGAAGAATTTTCAGGTACTTTCC delta-geobacter_metalli 451 GGCGGGAGCATCCTGGC-GGC--GAAGAAGCAGGGGCTCGCCAACTACTTCGTGCTGGCA delta-Pelobacter_propion 451 GCCGCCAGCATCCTGGC-CGC--CACTGGGGACGGGCTTGCCAACTATCTGGTGCTGGCG delta-geobacterFRC32 451 GCCGCCAGTATTCTTAT-GGC--AGCCAGGCAAAAGCTGGATAACTATCTAGTCCTGGCC proteo-others-Mariprofundus_fe 451 GCAGCCGTGCTGAAAAT-AGC--CAAAGCAATGCAACTGAATAACTTCACCGTATTCTGC alpha-Stappia_agg 451 GCAGTCGCGATCCTGCA-GGC--AAAGGCGGAGGGGTTGAAGAATTTCTCGGTCTTCTGC beta-Methylibium_petro 451 GCCCTGGCGATCCGCGA-CGC--CCACAAGGAGGGGCTGGCGAATTTCAGCGTGCTGTGC beta-Ralstonia_metall 451 GCGCTGGTGATCAAGCA-GGC--CGAGGCGGCCCGCGTCACCAACTTCAGTGTGCTGTGC beta-Rhodoferax_ferri 451 GCGCTGGCTATTTTGCA-GGC--ACACCAGGAACAATTGAGCAATTTCAGCGTGCTGTGC beta-dechloromonas_aro 451 GCGGTGGCGATCAAGCA-CGC--TGCCGCGCTGGGCCTGAAGAATTTCTCGGTGCTCTGC beta-Azoarcus 463 GCGCTGGTGCTGAAGCA-GGC--GCAGGCACTGGGGCTGAAGAACTTCAGCGTGCTGTGC beta-Burkholderia-viet 451 GCGCTGATCGTGCGCGA-CGC--GGCCGCTGCCGGCGTCGACAATTTCAGCGTGCTGTGC alpha-Magnetospirillum_mag 451 GCCGTGGCCATCAAACA-GGC--CCAGGCGCTGGACCTGAAGAATTTCAGCGTGTTCTGC alpha-Bradyrhizobium_jap 460 GCGGTCATGATCAGGAT-TGC--CGAGCACAAGCGGCTCGAGGGCCTTAGCGTGTTCTGC alpha-BradyrhizobiumBTAi1 451 GCGGTGATGATCCGCGC-CGC--TGAAAAAAAGAGCCTCACCAATTTCAGCGTGTTCTGC alpha-Rhodopseudomonas_palBisA 451 GCGCTGGCGCTGAAGCT-CGC--CATCGCCAAGCGGCTGACGAATTTCAGCGTGTTCTGC alpha-Rhodopseudomonas_palCGA0 451 GCATTGGCGATCAAGCT-CGC--GGCCGCCAAGCGGCTGACCAATTTTAGCGTGTTCTGC beta-Burkholderia_phy 451 GCTGTCGCACTGCTGAC-TGC--GCAGGCCGACCGGCAGGACAACTTCAGCGTGCTCAGC alpha-Rhodospirillum_rub11170 460 GCGGCGGCGATCAAGGC-CTC--CCTGGCCGCCGGTCTGACCAACTTCCATGAACTGGTC beta-Burkholderia_xeno 472 GCAATGACGGTGCTGCA-GGC--GCACGCCGGGCGCGTCGAAAACTTTTCGCTTTTCTGC alpha-Sphingopyxis_alask 463 GCCCTGACAATCCTGCA-GGC--CCAGGCAGAAGGGATCGGCAATTTCTCGCTGTTCTGC gamma-Shigella_flex 463 GCCATCACTCTGCAACA-GGC--GAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGC gamma-Shigella_sonn 463 GCCATCACCCTGCAACA-GGC--GAAAGCCCGCGATGTGCAGAATTTTTACTTCTTCTGC gamma-Shigela_dys 463 GCTATCACTCTGCAACA-GGC--GAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGC gamma-EcoliK12 463 GCTATCACTCTGCAACA-GGC--GAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGC gamma-Ecoli_CFT073 463 GCCATCACTCTGCAACA-GGC--GAAAGCCCGCGATGTGCAGAATTTTTACTTCTTCTGC gamma-salmonella_ent 463 GCCATCACGCTACAGCA-GGC--AAAACAGCGTGACGTGCGAAACTTCTACTTTTTCTGC gamma-salmonella_LT2 460 GCCATCACGCTGCAGCA-GGC--AAAACAGCGTGACGTGCGAAACTTCTACTTTTTTTGC gamma-enterobacter_638 463 GCCATTACCCTGCAACA-GGC--AAAAGCAAAAGGTCTCGATAACTTCTTCTTTTTTTGC gamma-erwinia_caro 463 GCACTCACCCTGCAACA-AGC--CAAGGCCGATGGCGTCGACAATTTTTACCTTTTTTGT gamma-Yersinia_ber 463 GCCTTAACCCTGCAACA-AGC--CAAACGGCTTGGGTTGACCAATTTCACTGTGTTCTGC gamma-Yersinia_int 463 GCCTTAACCCTGCAACA-AGC--CAAACGGCTTGGGTTGACCAATTTCACTGTGTTCTGC gamma-Yersinia_fred 463 GCCTTAACGCTACAACA-AGC--CAAGCGGCTTGGGCTGACCAATTTTAGTCTGTTCTGC gamma-Yersinia_moll 463 GCTTTAACCCTGCAACA-GGC--TAAGCGGCTGGGGCTAACCAACTTCAGCCTGTTCTGC gamma-Serratia_protea 463 GCGATCACCCTGCAGCA-GGC--CCGGCTGGAAGGGTTGAATAACTTCAGCGTGTTTTGC alpha-Rhodobacter_sphaer 463 GCCCTCTCGATCCAGCA-GGC--GGCGCGGGAGGAGCTCTTCAACTTCAGCGTCTTCTGC alpha-Rhodobacter_spaer17025 463 GCGCTCTCGATCCAGCG-GGC--GGCGCGCGAGGATCTGCGCAACTTCAGCGTCTTCTGC alpha-Roseovarius-2601 463 GCGCTGACCCTGCAGCA-GGC--GGCGCGCGAGGAGATCGGGAGTTTCAGCGTGTTCTGC alpha-Paracoccus_deni 463 GCGCTGGCCATCCAGCA-GGC--CGCGCGGGAAGGGTTGGGCAATTTCAGCGTCTTCTGC alpha-Xanhobacter_auto 463 GCGCTCACCGTGTTGCG-GGC--GGCGCGGGAGCGGGTTCGGAACTTCTCCCTGTTCTGC actinobacteria-corynebacterium 448 GCTGTAACACTCGAG-GCGGC--CAAACGCGAGAATGTGACCAACTTTGCGGTGTTTAGC Actinobacteria-RhodococcusRHA1 445 GCGGTGACGTTGGTGCGCGCG--CGGGCGCT-GGGCGTGAAGAACTTCAGTGTGTTCTGC Chloroflexi-Acidobacteria 442 GCCATGGCCGTATTCCA-AGC--CAAGCAGCAAGGCATCTCGAACTTCTCGGTGCTCGTC Chloroflexi-Solibacter_usi 442 GCCATGGCGGTTTCCCG-TGC--GAAAGCGCTGGGGCTGCGGAACTTTTCGCTGCTGGTT delta-proteo_MMS1 535 GCCATGGCGGTACTGCA-GGC--GGCCCGGCTGGGGCTGGCCAATTTCAGCCTGCTGGTC Cyan-SynechococcusWH5701 445 GCCCTGCTCGCCCACCA-GGCCTGGAGCAGTGGCGCACGG--AACCTCAGCCTGCTGGTG Cyan-Synechocystis6803 442 GCCATGACTCTCCACCA-AGCTAGGGCC--CAGGGAATTAGCAATTTCAGTTTACTTTGC chloroflexi-chloroflexus_agg 442 GCCATGGCGGTCTATCA-GGC--AGCCAGACTCGGCCTCAAGAACTTTTCGATGTTGGTC chloroflexi-chloroflexus_aur 442 GCGATGGCCGTCTACCA-GGC--GGCCCGCCTCGGCCTGAAGAACTTCTCGCTGCTGGTA Chloroflexi-Roseiflexus_cas 442 GCAATGGCAGTGTTGCA-GGC--GCACCGCCTGGGATTGCGCAATTTTTCGATGCTGGTG Chloroflexi-RoseiflexusRS-1 442 GCGATGGCGGTGTTGCA-GGC--GCACCGTCTGGGACTGCGCAACTTTTCGATGCTGGTA Actinobacteria-Mycobacterium_g 442 GCGATGACGGTGTACCA-GGC--GAAGAAGCTCGGCATCGAGAACTTCTCGCTGCTGGTC Actinobacteria-Mycobacterium_v 442 GCCATGACCGTCTACCA-AGC--GCGACGACTCGGCATCGACAACTTCTCGCTGCTGGTG Actinobacteria-FrankiaCcI3 442 GCGATGACGGTCTTCCA-GGC--GAAACGGCTCGACTTGGGCAACTTCTCCCTGCTGGTG Actinobacteria-FrankiaACN14a 442 GCGATGACCGTCTACCA-GGC--GAAGCGGTGCGGGGTGCGCAACTTCTCGCTGCTCGTC Actinobacteria-Streptomyces_av 442 GCCATGACGGTCCATCA-GGC--CCGTCGGCTCGGCATCCCGAACTTCAGCCTGCTGGTG Actinobacteria-Mycobacterium_s 442 GCCATGGCCGTGGTGCA-CGC--GAAACGTCTGGGCCTGAAAAACTTCTCGATGCTGGTC Chlorobi-Pelodictyon_lut 442 GCTATGGCGGTCCATCA-GGC--TGCGCGCGAAGGTATCGGCAATTTCAGCATCCTCGTC

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Chlorobi-Pelodicton_lut 442 GCCATGGCAGTCTGGCA-GGC--AGCGCGAGAAGGAATTGAAAATTTCAGCGTGCTGGTC chlorobi-chlorobium_lim 442 GCCATGGCCGTTCATCA-GGC--GGCCCGCGAAGGGATCGACAATTTTTTCGTGCTTTGC Chlorobi-Prosthecochloris_aest 442 GCTATGGCCATTAAACA-GGC--TGCAACGGAGGGGCTGGGGAATTTCAGTGCGTTGTCG chlorobi-chlorobium_tep 442 GCGATGGCCGTGCATCA-GGC--GGCACGGGAAGGGCTTACGAATTTCAGCGAGCTGGTG delta-Syntrophus_acid 454 GCATCAGCTATCCGCAAAGCC---GGGCTTAAAGGGCTGAACAACTATTCCGTCTTTTCC delta-desulfotalea_psy 442 GCCGCCACCATCATGGC-GGC--CAAGATACAAAAGATTAAGAATTTTGCTGTTTTTTCC chloroflexi-dehalococcoides_et 442 GCCGCTTCGGTAATACA-GGC--TCGTAATCAGGGGCTTGCTAATTATTATGTAATGTCA chloroflexi-dehalococcoides_CB 442 GCCGCTTCGGTAATTCA-GGC--GCGTAATCAAGGGCTTGATAATTATTATGTAATGTCG clostridia-desulfitobacterium_ 451 TGTGTAAGCATTGAACA-GGC--CCAAAAGGAAGATATCAGGAATTACAGTATTTTATCC Clostr-Moorella_therm 463 GCTGCAAGCCTTGCGAG-GGC--CCGGGCTAAAGGGGTGAAAAACTTTAGTCTATTTTCG Bacteroides-Robiginitalea_bif 442 GCTCTTTCAATTGATAA-GGC--TTATGACCTGGGACTTACCAACTATTCCATTTTAGCT epsilon-Helicobactr_acin 460 GCAAGCGTTTTACTCAA-CGC--AAAAAAAGAAAAGATCAAAAATCTTTTTTTCCACATT epsilon-Helicobacter_pylor 460 GCTAGCGTTTTATGGAG-CGC--CAAAAAAGAAAAAATTAATAACCTTTTTTTCCACATT gamma-PhotobacteriumSKA34 466 GCAGTTTTAGTTGAGCT-GGC--CGAGCAACGCCAAATCAATAATCTCTATTTTCACATT gamma-Vibrio_ang 466 GCAGTTTTAGTTGAACT-GGC--TGAGCAACGGCAAATCAATAACCTCTATTTTCACATT Gamma-Photobacterium_prof 463 GCTGTGCTGGTGGAATG-TGC--TGAACAGCAGCAGTTAGATAACCTATTTTTCCACATT epsilon-Wolinella_succ 460 GCAGCGCTTTTGGAGCG-AGC--CATGGGAGAAGGGGTCAAAAACCTCCTTTTTCATATC epsilon-campylobacer_con 442 GCAAATTTGGTTGAAAA-AGT--GGTGCAAGAGGGCATTAAAAATTTATACTTTCATATA Aquifex 469 GCCGTTCTTATAAAGAA-GGC--AAAGGAAGAAGGGATTAAAAACCTCTTCGTGGTTTCC gamma-Actinobacillus 466 GCGATAACATTACAGCA-AGC--CAAAAAGCAACAGGTAAATAACTTCTTTATTGTTTGC gamma-Manheimia-succ 466 GCCGTGACTTTGCAACA-GGC--AAAACGGCGGAATATTGCCAATTTTTGGATCGTTTCT gamma-Acidobacillus_succinogen 466 GCGGTAACGTTGCAACA-GGC--AAAAAAACAGAATGTGTCGAATTTCTTTGTGGTTTGC Cyano-Avar 451 GCTTTTACCATCCTCCA-AGC--TGCTTCAGAAAATATTACTAACTTTAGTATGTTTTCT Cyan-Nostoc7120 451 GCCCTTACCATCCTCCA-AGC--TGCTTCGGAAAATATTACTAACTTTAGTATGTTTTCT Cyan-Nodularia_spu 451 GCCCTCACCATATTGCA-AGC--AGCAGCCGAAAACATCACCAACTTTAGTATGTTTTCC Cyan-Lyngbya8106 463 GCCTTTACTATTCTACA-AGC--TGAAGCAGAAAATATCACAAACTTCAGCATTTTTTGC Cano-trichodesmium_ery 463 GCTTTAACTATTTTGCA-GGC--AGAAGCAGAAGGCGTCAAAAATTTCAGTATATTTAGT clostridia-caldicelluosiruptor 409 GCCCTGAGCCTTGAAAA-GGT--GATTGAAAAGGGACTCAAAAATGTTAAGTTTGCATGC clostridia-clostridium_beij 424 GCTTTAGCTGTAAAGAT-AGC--ACAGGAAAAAAATATAAGAAATATTTCATTTTTAATA Methanobacteriales-Methanospha 409 GCC---------AATGA-ATT--ATTAAATAATCCTCCTGAAAATTTTTCAGTACTTTCA Thermococcales-Pyrococcus_abys 409 GCC---------AATGA-ATT--ATTAAATAATCCTCCTGAAAATTTTTCAGTACTTTCA Methanococcales-Methanocaldoco 436 GGGGCTGAACTAATAAG-TTT--AAAAAATAAAGATGTTAATAACTTCTTTATCCTAAAT Methanococcales-Methanococcus_ 445 GCCGCAGAACTACTAAA-T-------AAAC-------CTGAAAATTTCTACATATTAAAT delta-Lawsonia_intra 445 GCAGCAACAATCCTTAT-GGC--AGAAAAAGAAGGTATTAATAATATTGCTGTTATTCCA epsilon-campylobacter_jej 442 GCTTTACTTCTTCAAAA-GGT--TATAGAAGAAAAAATAAATAATGTCTTTTTTCATATC epsilon-campylobacter_ups 442 GCTTTGCTTTTAGAAAA-AAT--CATCAAAGAAGATTTAAAAAATATTTCTATTTTTTCT clostridia-clostridium_therm 415 GCGCTGATGATTGAAGA-AAT--AATTAAGAATAATATAAAAAACCTGAAACTGATGACT clostridia-carboxidothermus_hy 436 GGAGCGACAATTTTGGA-AGC--ATATACGAAAAAACTTAAAAATTTTTCCATGTTTTTG Clostr-Halothermothrix_oren 445 GCACTAAGTATTATAAA-AGC--CAACCAACTGGGACTCAATAATTTTTCTATCTTACAA Methanosarcinales-Methanosarci 445 GCAGCAGCTCTTCTGAG-A-----------AATCCCCCCGAAAACTTCAGCCTCCTGGCC Methanosarcinales-Methanosarci 445 GCGGCTGCTCTTTTAAG-A-----------AAGACCCCCGAAAACTTCAGCCTCCTTACC Clostridia-Thermoanaerobacter_ 427 GCTTTGACGATAAAAGA-GGC--AAAAGAGAGAAAAATAAAAAATTACAGTGTGTATTCG clostridia-clostridium_cell 445 GCACTCTCCGTACTAAA-GGC--AAAAAGCGAGGGTATAAAAAATTATTCAGTGCTTACT cyano-crocosphaera_wat 442 GCTATGGCAGTTTATCA-GGC--TGATCAACAAGGAATTGAAAACCTCTCTTTATTAGTT Bacteroides_Flavobacteriu_john 439 GCGCTGGCCGTTCTTCA-TGC--CCAAAAACTGGGTTTAACCAATTTTAGTTTATTGGTT

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Proteo-others-MagentococcusMC1 487 GCGCTGCGTTTAAGTTGGCC------------GGTGGTGGATACCATGTTGTCGCAG--- beta-Nitrospira_multi 484 TCAGCGCGGCGCACATGGCC------------TGCGGT---CGCAATGCTGCTTGAT--- beta-Thiobacillus_denitri 481 TCCGGGCGCCTGACCTGGCC------------CGCGGT---CGCGATGCTGCTCGAC--- alpha-Acidiphilum 490 GCGGCACGGCTCACCTGGCC------------CGCCGT---CGCCATGCTGCTCGCC--- Thermococcales-Pyrococcus_fur 514 GTTCATAGGTTAACCCCTCC------------TGCAGTTGAAGCTCTCCTAAATGCG--- Thermococcales-Pyrococcus_hori 514 GTCCACAGGTTGACCCCTCC------------AGCGGTTGAAGCTCTCCTCAAAGCG--- Thermococcales-Thermococcus_ko 526 GTCCACAGGCTAACCCCGCC------------AGCCGTTGAGGTGCTCCTCAAGCAG--- Archaeoglobales-Archaeoglobus 511 GCCCACCGCTTCTTCATCCC------------TGCAGTGCATGCGCTCTGCGAA------ Methanopyrales-Methanopyrus_ka 466 GCTCACCGGTTGATACCACC------------TGCGATGGAGTGGTTGCTGGAGTCG--- Methanomicrobiales-Methanospir 448 TGTCATCGCCTGGTTCCCCC------------CGCAATGGAGTGGTTGCTATCCCAG--- Sulfolobales-Metallosphaera_se 508 TCCTATAGATATACCCCGGC-----TATTCAGGGCTCAGTAATGGATTC--GAATGT--- Thermoproteales-Pyrobaculum_is 544 CTAGTCAAACTGACCCCGCCGGCGATGTTCTACGCCCTTGACATGGTTAAAGAAAAG--- Methanobacteriales-Methanother 457 TGCCACAGGCTCATACCACC------------CGCCCTCAGGTTTCTCATAGAGTCA--- Methanomicrobiales-Methanocull 451 TGCCACCGGCTCGTCCCGCCCGCGATGGCATGGCTCCT---CGCCCAGGGCGA------- Thermoproteales-Thermofilum_pe 568 CTCGTTAAGCTCACGCCTCCAGCCATGTTCTACTCCTTGGACATCCTGCGCGACAAG--- delta-Anaeromyxobacter 511 GTGCACAAGTACGTCCCGGC------------GGCGATGGAGATCGTGGCCGCCTCG--- delta-desulfovibrio_des 502 TTTCACAAACTGGTGCCCCC------------CGCTCTGGGCGCGCTGCTTTCCGAC--- delta-geobacter_metalli 508 GCCCACAAGACCATGCCCAA------------GCCCATGGGGGTCCTCTCCGCCGAC--- delta-Pelobacter_propion 508 GCCCACAAGACCATTCCCTT------------GCCCATGGAGCTACTCTCCAGCGAC--- delta-geobacterFRC32 508 GGCCACAAAACAATGCCTAA------------AGCCATGGAAATGCTGGCGGCGGAA--- proteo-others-Mariprofundus_fe 508 AATCATGTCCAAACACCGCCTGCGATGATGCATATCCT---CTCTGCG--GGCGAAGCCG alpha-Stappia_agg 508 AACCACGTTCTGACGCCTCCGGCCATCGCCCATATCGT---CAACGCTCCGGCGGAACCA beta-Methylibium_petro 508 TGCCATGTGCTCACGCCGTCGGCGATCACGCACATCCT---CGAGTCGCCCGAAGTACGG beta-Ralstonia_metall 508 AACCACGTGCTGACGCCTTCGGCCATCATCACCATCCT---CGAGTCGCCGGAGGTGCGC beta-Rhodoferax_ferri 508 TGCCATGTGCTGACACCCGCGGCGATCAGCCAGATTCT---GGAGTCGCCCGAAGTACGG beta-dechloromonas_aro 508 TGCCATGTGCTGACGCCATCGGCCATTTCCAGCATCCT---CGAATCGCCGGAGGTTCGC beta-Azoarcus 520 AACCACGTGCTGACCCCGGCGGCGATCGTCGCCATCCT---CGAACACCCCGAGG----- beta-Burkholderia-viet 508 TGCCACGTGCTGACGCCCGCCGCGATCACGCATCTGCT---CGCGGCGCCGGCCGTG--- alpha-Magnetospirillum_mag 508 AACCACGTGCTGACGCCGTCGGCCATCACCCAGATTCT---CGACAGCCCGGAAGTGCGC alpha-Bradyrhizobium_jap 517 AATCACGTTCTTACGCCCTCTGCGATGCACCGCATTCT---CGAGAACCCGAAGATCCGC alpha-BradyrhizobiumBTAi1 508 AACCACGTCCTGACGCCACCGGCGATGCGCGCGATCCT---CGACAGC-----GACACGC alpha-Rhodopseudomonas_palBisA 508 AACCACGTGCTGACGCCGCCGGCGATCCGCGGCATTCT---GGAACATCCTGATATCGAC alpha-Rhodopseudomonas_palCGA0 508 AACCACGTGCTGACGCCGCCGGCGATCCGCGGCATCCT---CGAACATCCCGATCTCGAT beta-Burkholderia_phy 508 AACCACGTGCTCACGCCCGCCGCAATGCGCCATCTGCT---TGCCGAC------------ alpha-Rhodospirillum_rub11170 517 TGCCATGTGCTGACGCCGCCGGCGATGGCCGCCATCCTGGCCGAGGAGGCCACCGCCGCG beta-Burkholderia_xeno 529 AACCACATCACTATCGTTCCGACGATCAAGGCGATTCT---CGACTCACCGGACC----- alpha-Sphingopyxis_alask 520 AACCACATCACCATCATCCCCACGATCAAGGCGATCCT---CGATTCACCCGATA----- gamma-Shigella_flex 520 CAGCACATTACGCTTATCCCGACGCTGCGCAGTTTGCT---GGAACAGCCGGATA----- gamma-Shigella_sonn 520 CAGCATATTACGCTTATCCCGACGCTGCGCAGTTTGCT---GGAACAGCCGGATA----- gamma-Shigela_dys 520 CAGCACATTACGCTTATCCCGACGCTTCGCAGTTTGCT---GGAACAGCCGGATA----- gamma-EcoliK12 520 CAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCT---GGAACAGCCGGATA----- gamma-Ecoli_CFT073 520 CAGCACATTACGCTTATCCCGACGCTGCGCAGTTTGCT---GGAACAGCCAGATA----- gamma-salmonella_ent 520 CAGCACATTACACTGATCCCGACGCTGCGTAGCCTACT---GGAGCAGCCGGACA----- gamma-salmonella_LT2 517 CAGCACATTACGCTGATCCCGACGCTGCGTAGCCTACT---GGAGCAGCCGGACA----- gamma-enterobacter_638 520 CAGCACATCACGCTGATCCCCACGCTGCGTAGCCTGTT---GGAAGAACCCGATA----- gamma-erwinia_caro 520 CAGCACATTACTATCATCCCCACCCTACGCAGCCTGCT---GTTACAGCCGGATA----- gamma-Yersinia_ber 520 CAGCACATCACCATCATTCCTACCCTGCGTAGCTTGCT---GCAACAACCGGATG----- gamma-Yersinia_int 520 CAGCACATCACCATCATTCCTACCCTGCGTAGCTTGCT---GCAACAACCGGATG----- gamma-Yersinia_fred 520 CAGCACATTACTATTATTCCGACTTTGCGCAGCCTGCT---ACAGCAGCCAGATG----- gamma-Yersinia_moll 520 CAGCACATCACCATTATTCCGACACTGCGTAGTCTGCT---ACAACAGCCGGATG----- gamma-Serratia_protea 520 CAGCACATCAGCCTGATCCCCACCTTGCGCAGCCTGTT---GCAACAGCCGGATG----- alpha-Rhodobacter_sphaer 520 AACCACATCACCGTGCCCGAGCCGATCCGGGCGTTGCT---CGACGATCCCGACA----- alpha-Rhodobacter_spaer17025 520 AACCACATCACGGTGCCCGAGCCGATCCGGGCGCTGCT---CGGCGATCCCGACA----- alpha-Roseovarius-2601 520 AACCACATCACCGTGCCGCCGCCGATCAAGGCGTTGCT---CGATGATCCGCACA----- alpha-Paracoccus_deni 520 AACCATATCACCGTGCCCGAACCCATCCACGCCCTGCT---GAAGGATCCGCATA----- alpha-Xanhobacter_auto 520 AACCACATCACCATCATCCCCACGCTCCGGGCGCTGCT---GGAGCAGCCGGACC----- actinobacteria-corynebacterium 505 AACCATGTGACCATCGAGCCACC--TCTA---CGTGTT---TTGGTCAACGACAC----- Actinobacteria-RhodococcusRHA1 502 AACCACGTCACCATCGTGCCGCCGATCAAGGCCATCCT---CGAGTCCCCCGACC----- Chloroflexi-Acidobacteria 499 TCGCACGTGCTTGTGCCGCCCGCGATTGAGGCCGTGCT---GAGCGCACCCGACA----- Chloroflexi-Solibacter_usi 499 TCGCACGTAGTGGTGCCCCCTTCGATGACCGGGATTCT---GCAATCGCCGGGGA----- delta-proteo_MMS1 592 AGCCACGTCCTGGTGCCGCCGGCCATCGCCGCCCTGCT---GGCGGCGCCGGATA----- Cyan-SynechococcusWH5701 502 TCACACGTGCGGGTGCCGCCGGCGATGGAGGCGATCCT---GGCCGCGCCGGAAG----- Cyan-Synechocystis6803 499 GCCCATGTATTGGTGCCCCCGGCTATGGAGGCTTTATT---AGGCAATCCCAATT----- chloroflexi-chloroflexus_agg 499 TCACACGTCCTGGTACCACCGGCAATCAGTGCGATTAT---GGAGTCGCCGAACA----- chloroflexi-chloroflexus_aur 499 TCTCACGTGCTGGTGCCACCGGCGATCCGGGCGATTAT---GGAATCGCCTTCTA----- Chloroflexi-Roseiflexus_cas 499 TCGCACGTGCTGGTGCCACCCGCCATCTCCGCCATCAT---GGAGTCGCCGACGA----- Chloroflexi-RoseiflexusRS-1 499 TCCCACGTTCTGGTGCCGCCAGCGATTTCAGCCATCAT---GGAGTCGCCGACAA----- Actinobacteria-Mycobacterium_g 499 TCCCATGTGCTGGTGCCGCCGGCGATCGCGGCGATCAT---GGAGTCCCCGACCT----- Actinobacteria-Mycobacterium_v 499 TCCCACGTGCTGGTGCCGCCTGCGATCTCGGCGATCAT---GGAATCGCCGACGT----- Actinobacteria-FrankiaCcI3 499 TCGCACGTGCTGGTACCGCCGGCGATTGCCGCGATCAT---GGAGTCGCCGAGCT----- Actinobacteria-FrankiaACN14a 499 TCACACGTCCTGGTCCCGCCGGCGATGGCCGCGATCAT---GGAATCGCCGAGCT----- Actinobacteria-Streptomyces_av 499 TCCCACGTCCGGGTGCCGCCCGCCATCGAGGCGATCAT---GTCGGCGCCGAACT----- Actinobacteria-Mycobacterium_s 499 TCACACGTGCTGGTGCCGCCGGCGATGACGGCCATCCT---CAGTTCACCCAGCA----- Chlorobi-Pelodictyon_lut 499 AGCCAGGTCATGGTTCCTCCGGCGATGCGTGCCATTCT---CCGAATGCCGGCCA-----

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Chlorobi-Pelodicton_lut 499 AGCCAGGTCATGGTGCCGCCAGCCATGCGAGCCATTCT---TTCATCTCCCGAAA----- chlorobi-chlorobium_lim 499 AGTCAGGCGCTGGTTCCTCCGGCAATGCGCGCGATTCT---CTCGTCACCGGAGA----- Chlorobi-Prosthecochloris_aest 499 AGCCAGGTGCTTGTACCTCCGGCGATGCGTGCTATTCT---CTCTTCTCCCGATA----- chlorobi-chlorobium_tep 499 AGCCAGGTGATGGTGCCGCCGGCGATGCGCGCAATGCT---GTCGTCGCCCGGCA----- delta-Syntrophus_acid 511 GTACACAAGACCGTCCCGCAGGCGATCTCCGCCCTCAT---CGAGGATCCCGAAC----- delta-desulfotalea_psy 499 ACCCAAAAAACAATGCCTGCCCCCCTGATTGCCCTGCTT--CAGGACCCCGAACT----- chloroflexi-dehalococcoides_et 499 ATTCACAAAATAACCCCTCCCGTTATGCGGGCGATACT---GGATGCAGGTGAAA----- chloroflexi-dehalococcoides_CB 499 CTCCATAAAATAACCCCTCCCGTCATGCGGGCTATACT---GGACGCGGGTGAAA----- clostridia-desulfitobacterium_ 508 ATGCATAAGCTTGTGCCTCCTGTGCTGCGCGCCTTAGC---AGAGGATCCGGAGA----- Clostr-Moorella_therm 520 GCCCATAAACTCATCCCCCCGGCCCTTCGCGCCGTTCT---CAGTGACCCCGAAT----- Bacteroides-Robiginitalea_bif 499 TCCCAGGTGTTGGTACCGCCGGCTATGGAAGCGGTCAT---CGAAGATGAGGAAA----- epsilon-Helicobactr_acin 517 AACCACATTTTAGTGCCCCCAAGCGTGAGCGCGATTTTAGAAGATCCAACATGCC----- epsilon-Helicobacter_pylor 517 AACCACATTCTAGTGCCTCCCAGCGTGAGCGCGATTTTAAAAGATCCAGCATGCC----- gamma-PhotobacteriumSKA34 523 AATCATGTATTAGTGCCGCCAGCAGTTGATGCGGTAATGGCTGATCCAGGGGTTA----- gamma-Vibrio_ang 523 AACCATGTTTTAGTGCCGCCAGCCGTTGATGCAGTAATGGCTGATCCAGCGGTAA----- Gamma-Photobacterium_prof 520 AACCATGTGCTGGTGATGCCGGCGATGGAAGCGGTGCTCGCTGGTGGCGAGTCTC----- epsilon-Wolinella_succ 517 AATCATATCACGGTTCCAGAGCCCGTGAGAGCGATTCTAGATGATCCCTCCTCAA----- epsilon-campylobacer_con 499 AATCACGTAACCGTCCCAGCTCCAGTTAGAGCTATAATGAGCGATGAAAATGTAA----- Aquifex 526 AACCACGTTATAACGCCCGCTGCGATCCAGCACATACTTAACGCCCCGGAAATCAGG--- gamma-Actinobacillus 523 CAAAACATTACGATTATTCCTACATTACGAGAATTATT---ACGACAAGAGCAAG----- gamma-Manheimia-succ 523 CAAAACATTACTATTATTCCAACATTGCGCAGTTTGCT---TTCGCAGGATCAAA----- gamma-Acidobacillus_succinogen 523 CAGAATATCACCATTGTTCCCACTTTGCGCAGCCTGCT---GTTGCAGGAACAAA----- Cyano-Avar 508 AATCACGTCCTCGTCATTCCCGCCCTGCAAGCTTTATT---AAATAACCCTGATT----- Cyan-Nostoc7120 508 AATCACGTCCTCGTAATTCCCGCCTTGCAAGCTTTATT---AAATAATCCTGATT----- Cyan-Nodularia_spu 508 AACCATGTTCTCGTAATTCCCGCCCTTCAAGCCCTACT---AGATAACGCAGATT----- Cyan-Lyngbya8106 520 AATCATGTGATTGTAGTTCCGGCATTAGAAGCATTACT---AGATAACCCAGATT----- Cano-trichodesmium_ery 520 AACCATGTTTTGGTTGTACCGGCTCTAGAGGCATTACT---TAGCAACCCAGATT----- clostridia-caldicelluosiruptor 466 GAACTTAAGACAATTGATGAACCTCTTAAAAGCTTTCT---TCAAAACCATA--T----- clostridia-clostridium_beij 481 GGAATGAAAAGAATGGAGCCAATACTCCATTATATACT---TAAGCAGCCTAATC----- Methanobacteriales-Methanospha 457 AGTCATAGATTAATACCTCCTGCTTTAGATTTCTTAGT---TCATGATGAAG-------- Thermococcales-Pyrococcus_abys 457 AGTCATAGATTAATACCTCCTGCTTTAGATTTCTTAGT---TCATGATGAAG-------- Methanococcales-Methanocaldoco 493 TGCCACAGGCAGACTCCTCCAGTTATGGAGTTCTTGTT---AAATGAGGGAGTTT----- Methanococcales-Methanococcus_ 490 TCACACAGGCAGACCCCACCAGTAATGGGATTTTTACT---CGGCGGTGGAACTTCA--- delta-Lawsonia_intra 502 TGCCATAAACTAGTTCCACCAGCACTTGAAATGTTACT---AGCAGATCCAGATT----- epsilon-campylobacter_jej 499 AATCATATCACGGTTCCAGCACCTGTGGAAGCGATCAT---GAATGATGAAAATG----- epsilon-campylobacter_ups 499 AATCATATCACCGTTCCAGCTCCAGTAGAGGCGATAAT---GAGTGATGAAAATG----- clostridia-clostridium_therm 472 TCGATTAAGACCATTATACCTGCGATTTCATATATTTG---CGAAAATGAAAA------- clostridia-carboxidothermus_hy 493 GCGGTAAAAACAGTGCCTCCTGCCCTTGGATTTTTGGT---GGAGCACAACAGCT----- Clostr-Halothermothrix_oren 502 TCACTTAAAATAATGCCTCCAGTATTACAGGAACTGGT---TTTAGATAAAGATT----- Methanosarcinales-Methanosarci 493 TCCCAGAAACAGACTCCTCCTGCAATTGAGCTTCTTGC---CAGTGATACCG-------- Methanosarcinales-Methanosarci 493 TCGCAGAAGCAAACCCCTCCTGCAGTCGAATTCCTTGC---CAGGGACGCAG-------- Clostridia-Thermoanaerobacter_ 484 CTTCACAAGACAATGCCTGAAGCCTTAAGAGCTCTTCT---GGTAGGGGGTTCTA----- clostridia-clostridium_cell 502 GCAAATAAGACTATGCCCGAGGCAATCAAGGCCTTGGC---AGATGACAAGGAAC----- cyano-crocosphaera_wat 499 TCTCATGTCTTAGTTCCCCCAGCTATGTCAGCCCTTTT---ATCATCGCCTAATTG---- Bacteroides_Flavobacteriu_john 496 TCTCATGTTTTAGTTCCGCCTGCAATGGAAGCTATTTT---ATCA--GATAAATTC----

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Proteo-others-MagentococcusMC1 532 ---CATGAGCGGCGCCCTTTTAAAGCCATTATTGCCCCGGGTCACGTGGCGGCCATTGTC beta-Nitrospira_multi 526 ---TCTGATGCACCAGGCTTCGATGGGTTGGTGGCGCCCGGTCATGTTTCCACTGTCATG beta-Thiobacillus_denitri 523 ---TCGGGCAGCCCCGGCTTCGACGCGCTGATCGCCCCCGGCCACGTGTCGACCGTGATG alpha-Acidiphilum 532 ---GAGGAGGACAACGCGCTGGACGCCCTGGTCGCCCCCGGCCACGTGGCGACGGTGATG Thermococcales-Pyrococcus_fur 559 ---GGGACTGTTT------TTCACGGTTTAATAGATCCTGGTCATGTCTCTACAATAATT Thermococcales-Pyrococcus_hori 559 ---GGAACGGTAT------TTCAGGGATTAATTACTCCAGGACATGTATCTACGATCATA Thermococcales-Thermococcus_ko 571 ---GGCACGGTCT------TTCAGGGCCTCATAGCCCCTGGTCACGTCTCCACGATAATC Archaeoglobales-Archaeoglobus 553 ---AACACGACCG------TTGATGCCTTCATAAACCCCGGACACGTCTCAACGATAATC Methanopyrales-Methanopyrus_ka 511 ---GGCGAGTGCCGTC---TCGACGCGTTCATATGTCCCGGTCACGTGTCCACGATCATC Methanomicrobiales-Methanospir 493 ---GGTGAGGCAGCAC---TGCACGGATTTCTCCTTCCCGGCCACGTACTGACCGTTACC Sulfolobales-Metallosphaera_se 558 ---CTTAGGGATAGA--------TGCCTTCATATCTGCGGGTCATTCGGTAACTGTGACT Thermoproteales-Pyrobaculum_is 601 ---CCTACCGACTATCCCATCTCTGGCGTCATCGCGCCGGGCCACGTCTCAACAATAATA Methanobacteriales-Methanother 502 ---GGTGAGGTGAACC---TCAACGCCCTCATAGAACCAGGTCACGTGTCAACCATAATC Methanomicrobiales-Methanocull 501 --------GGCGTCGCTCGAC---GGGTTCCTCCTCCCGGGACACGTCTGCGCGGTGAT- Thermoproteales-Thermofilum_pe 625 ---CCCACGGAGCCGCCTGTCATGGGGGTGATAGCGCCGGGCCACGTGTCGACGGTTACC delta-Anaeromyxobacter 556 ---AAGGAGACGCACA---TCGAGGGCTACGTGGCCGCCGGCCACGCCGCGATCATCACC delta-desulfovibrio_des 547 ---CCTGACTGTGCAG---TGGATGCTTTTCTGCTGCCGGGACATGTTTCCACCATTCTG delta-geobacter_metalli 553 ---CCGGAGCTGAAGA---TCGACGGCTACATCTGCCCCGCCCACGTGAGCACCATCACC delta-Pelobacter_propion 553 ---CCGGAACTGAAGA---TCGACGGCTATATCTGCCCCGCCCATGTGAGCGCCATCATC delta-geobacterFRC32 553 ---CCGGCTGCGGCAA---TCCATGGCTTTATCTGCCCGGCCCATGTGAGCGCCGTCATC proteo-others-Mariprofundus_fe 563 GGA----------------TCGACGGCATCCTGGGGCCCTCGCATGTTAGTGCAATTATC alpha-Stappia_agg 565 GGAGAGGCGCCGGTCAAAATCGACGGTTTCCTGGGGCCTTCACATGTCAGCTCGGTCATT beta-Methylibium_petro 565 CAGTTCGGCACGGTGCCGATCGATGGCTTCGTGGGGCCGGCACACGTCAGCATCGTGATC beta-Ralstonia_metall 565 AAGCTGGGTACCGTGCCGCTGGACGGCTTCGTCGGCCCCGCCCATGTCAGCACCGTGATC beta-Rhodoferax_ferri 565 CAGTGGGGCACGGTGCCGATTGACGGCTTCATCGGCCCGGCGCATGTGTCAACCGTGATT beta-dechloromonas_aro 565 CAGTGGGGCACCGTGCCGCTCGACGGCTTCATTGGTCCAGCCCATGTATCGACCATCATC beta-Azoarcus 572 ----GCAGCGGCGTGCAGCTCGACGGCTTCGTCGGCCCGGCGCACGTCTCCACGGTGATC beta-Burkholderia-viet 562 GCGGCCGACGCCGTGCCGCTCGACGGCTTCATCGGCCCCGCGCACGTCAGCATCGTGATC alpha-Magnetospirillum_mag 565 GAGCTGGGCACGGTCAAGCTGGACGCCTTCATCGGCCCGGCCCATGTGTCAACCATCATC alpha-Bradyrhizobium_jap 574 AACACGGGCGGGGTCCCCATTGACGGCTTCATCGGGCCTGCACATGTCAGCACCATTGTC alpha-BradyrhizobiumBTAi1 560 ----CGATCGAGATC------GACGGCTTTGTCGGACCGGCCCATGTCTCGACCGTGATC alpha-Rhodopseudomonas_palBisA 565 GGCCTGGAAGGCCCCAAGCTCGACGGCTTCATCGGGCCGGCGCATGTCAGCGCGGTGATC alpha-Rhodopseudomonas_palCGA0 565 GGTCAGCCGGGGCCGCGCCTCGACGGCTTCATCGGTCCCGCGCATGTCAGCGCAGTGATC beta-Burkholderia_phy 553 ------AACGATGCAAATCTCGATGGCTTCATCGGCCCGTCGCACGTCAGCACGATCATC alpha-Rhodospirillum_rub11170 577 ACCGATGTCCGGCCGCGTCTGGATGGGCTGATCGGCCCCGGCCATGTGGCTTTGATCACC beta-Burkholderia_xeno 581 -------------TGCGCCTCGACGGATTTCTCGGCCCGGGGCACGTCAGCATGGTGATC alpha-Sphingopyxis_alask 572 -------------TGGGGATCGATGGCTTTCTCGGGCCGGGCCATGTCTCGATGGTCATC gamma-Shigella_flex 572 -------------ACGGTATCGACGCGTTCCTCGCGCCGGGCCACGTTAGTATGGTTATC gamma-Shigella_sonn 572 -------------ACGGTATCGACGCGTTCCTCGCGCCGGGCCACGTTAGTATGGTTATC gamma-Shigela_dys 572 -------------ACGGTATCGACGCGTTCCTCGCGCCGGGCCACGTTAGTATGGTTATC gamma-EcoliK12 572 -------------ACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATC gamma-Ecoli_CFT073 572 -------------ACGGTATCGACGCGTTCCTCGCGCCGGGCCACGTCAGTATGGTTATC gamma-salmonella_ent 572 -------------ACGGCATTGACGCCTTTCTTGCGCCAGGCCACGTCAGCATGGTCATC gamma-salmonella_LT2 569 -------------ACGGCATTGACGCCTTTCTTGCGCCAGGCCACGTCAGCATGGTCATC gamma-enterobacter_638 572 -------------ACGGAATTGATGCTTTTTTAGCACCCGGTCACGTCAGTATGGTGATT gamma-erwinia_caro 572 -------------ACAGCATTGACGCTTTTATCGCGCCGGGACATGTCAGCATGGTAATC gamma-Yersinia_ber 572 -------------TGCGCATCGACGCTTTTCTGGCTCCAGGTCATGTCAGTATGGTGATT gamma-Yersinia_int 572 -------------TGCGCATCGACGCTTTTCTGGCTCCAGGTCATGTCAGTATGGTGATT gamma-Yersinia_fred 572 -------------TGCGGATCGACGGTTTTCTGGCTCCTGGCCATGTCAGCATGGTGATC gamma-Yersinia_moll 572 -------------TGCGCATCGACGGTTTTCTGGCCCCCGGCCATGTCAGCATGGTGATC gamma-Serratia_protea 572 -------------TCCGCATCGACGGCTTTCTGGCGCCCGGCCACGTCAGTATGGTGATT alpha-Rhodobacter_sphaer 572 -------------TGCGGCTCGACGGCTTCATCGGGCCCGGCCATGTCTCGATGGTGATC alpha-Rhodobacter_spaer17025 572 -------------TGATGCTCGACGGCTTCATCGGGCCGGGCCATGTGAGCATGGTGATC alpha-Roseovarius-2601 572 -------------TGCAGCTCGACGGCTTCGTCGGGCCGGGCCATGTCTCGATGGTGATC alpha-Paracoccus_deni 572 -------------TGGTGCTGGACGGGTTCATCGGGCCGGGTCATGTCTCGATGGTCATC alpha-Xanhobacter_auto 572 -------------TCGGCATCGACGGCTTCATCGGGCCGGGGCATGTCTCCATGGTCATC actinobacteria-corynebacterium 552 -------------CAAAGTTGATGG-CTTTATCGGCCCAGGCCACGTGGCCACCATCGTC Actinobacteria-RhodococcusRHA1 554 -------------TGCGGTTGTCGGGCTTCCTCGGCCCGGGCCACGTGTCCACTGTGGTT Chloroflexi-Acidobacteria 551 -------------ACCGCACGCAAGCGTTTCTGGCTGCCGGACACGTCTGCACTGTGATG Chloroflexi-Solibacter_usi 551 -------------ATCGCGTACAGGGATTCCTGGGACCTGGACACGTGTGCGCGGTGATG delta-proteo_MMS1 644 -------------ATCGGGTGCAGGGTTTCCTGGCCGCCGGTCACGTCTGCACGGTGACC Cyan-SynechococcusWH5701 554 -------------GCCGGGTGCAGGGCTTTCTGGCCGCGGGTCATGTTTGCACCGTGATG Cyan-Synechocystis6803 551 -------------CCCTCGTGCAGGGCTTTTTGGCGGCAGGGCATGTCTGTACGGTGACC chloroflexi-chloroflexus_agg 551 -------------ACCGCGTCCAAGGCTTTCTAGCCGCTGGTCATGTCTGCAGCGTCATG chloroflexi-chloroflexus_aur 551 -------------ACCGCGTCCAGGGATTTCTGGCCGCCGGTCATGTCTGTAGTGTGATG Chloroflexi-Roseiflexus_cas 551 -------------ACCGTGTGCAAGGATTCCTGGCAGCCGGGCATGTGTGCAGCGTGATG Chloroflexi-RoseiflexusRS-1 551 -------------ACCGGGTGCAGGGGTTTCTGGCTGCCGGGCATGTGTGCAGCGTCATG Actinobacteria-Mycobacterium_g 551 -------------GCCGCGTCCAGGCGTTCCTCGCGGCAGGCCACGTGTGCACGGTGATG Actinobacteria-Mycobacterium_v 551 -------------GCCGCGTGCAGGCGTTCCTGGCGGCCGGCCACGTGTGCGCGGTGATG Actinobacteria-FrankiaCcI3 551 -------------GCCGCGTCCAGGGGTTCCTGGCCGCTGGTCACGTCTGCAGCGTGATG Actinobacteria-FrankiaACN14a 551 -------------GCCGGGTCCAGGGGTTCCTCGCCGCCGGCCACGTGTGCAGCGTGATG Actinobacteria-Streptomyces_av 551 -------------GCCGCGTCCAGGCCTTCCTCGCGGCCGGGCACGTGTGCAGCGTGATG Actinobacteria-Mycobacterium_s 551 -------------ACCGGGTCCAGGGATTCCTGGCAGCCGGGCACGTGTGCTCGGTGATG Chlorobi-Pelodictyon_lut 551 -------------ACCGCGTCCAGGGCTTTCTTGCTGCCGGCCATGTGTGCGCCGTCATG

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Chlorobi-Pelodicton_lut 551 -------------ACAGGGTTCAGGGCTTTCTTGCAGCAGGGCACGTTTGCGCAGTCATG chlorobi-chlorobium_lim 551 -------------ATCGTGTGGAGGGTTTCCTTGCCGCCGGTCACGTCTGTGCAGTGACC Chlorobi-Prosthecochloris_aest 551 -------------ACCTCGTCCAGGGCTTTCTTGCGGCCGGCCATGTGTGTACGATAACC chlorobi-chlorobium_tep 551 -------------ACCGCGTGCAGGGGTTCCTTGCCGCAGGGCACGTCTGCGCCATCATG delta-Syntrophus_acid 563 -------------TCTCCATTGACGGCTTTATCTGTCCCGGCCATGTCAGCGTCATCACC delta-desulfotalea_psy 552 --------------CAAAATCGACGGTCTGCTCTGCCCCGGCCATGTTTCTGCCATTATC chloroflexi-dehalococcoides_et 551 -------------CCCGCCTTTCGGGCATAATCTGCCCCGGCCATGTTTCTTCTATTACC chloroflexi-dehalococcoides_CB 551 -------------CCCGTCTTTCGGGCATAATCTGCCCCGGACATGTTTCTTCCATTACC clostridia-desulfitobacterium_ 560 -------------CGGAAGTGGATGGGTTTCTGGATCCCGGTCATGCCTGCGCTATTATT Clostr-Moorella_therm 572 -------------TAAAACTGGACGGGCTTATCTTGCCGGGTCATGTTAGCGCCGTTACT Bacteroides-Robiginitalea_bif 551 -------------GTAAAATTGATGCTTTTTTGGCCGCCGGCCACGTATGCACAATTATG epsilon-Helicobactr_acin 572 ----------------CAATCAACGCCCTTTTAGCCCCTAGCCATGTGAGCGTGATAAGC epsilon-Helicobacter_pylor 572 ----------------AGATTAACGCCCTTTTAGCCCCTAGCCATGTGAGCGTGATCAGC gamma-PhotobacteriumSKA34 578 ----------------AAGTGAATGCATTTATTGGTCCGTCACATGTGAGTGTGATTGAA gamma-Vibrio_ang 578 ----------------AAGTGAATGCGTTTATTGGCCCGTCACATGTGAGTGTGATTGAA Gamma-Photobacterium_prof 575 ----------------CAATCAACGCATTTATTGGTCCGTCCCATGTGAGTGTGATCAGT epsilon-Wolinella_succ 572 ----------------CCCTTGACGCCCTCATTGGCCCCTCCCATGTGAGCGTGATTGCA epsilon-campylobacer_con 554 ----------------GGATAGACGCATTTTTGGGTCCAAGTCACGTGAGCGTGATCACG Aquifex 583 GAGATAGGAAAAGTGGAGATAGACGCCTTCATAGGACCGGGGCACGTGAGCGTGATAATA gamma-Actinobacillus 575 -------------TATTAATCGACGGCTTTATCGCACCGGGTCACGTCAGTATGATTATC gamma-Manheimia-succ 575 -------------TTAAAATTGACGGTTTTATTGCGCCGGGTCATGTGAGTATGGTGATT gamma-Acidobacillus_succinogen 575 -------------TCAAAATAGACGGTTTTATCGCACCGGGGCATGTCAGCATGGTAATC Cyano-Avar 560 -------------TGCAGCTAGATGGCTTTATTGGCCCCGGTCATGTGAGTATGGTAATT Cyan-Nostoc7120 560 -------------TGCAGCTAGATGGCTTTATTGGCCCTGGTCATGTGAGTATGGTAATT Cyan-Nodularia_spu 560 -------------TACAACTAGATGGATTTATCGGCCCTGGTCATGTCAGCATGGTAATT Cyan-Lyngbya8106 572 -------------TAGAACTAGATGGATTTATTGGCCCTGGTCATGTTAGCATGGTTATT Cano-trichodesmium_ery 572 -------------TAGAATTACAAGGTTTTGTTGGGCCTGGTCATGTGAGTATGGTTATT clostridia-caldicelluosiruptor 516 -------------ACGG-GTTGATGGCATAATTTTACCTGGACATGTTGCGACAGTTATT clostridia-clostridium_beij 533 -------------ACAATATAAATGGATTAATGTGTCCAGGACATGTAGCAGCAGTAAAG Methanobacteriales-Methanospha 506 -------------TTGAATTAGATGGTTTAATAGAACCAGGTCATGTATGTACAATAATT Thermococcales-Pyrococcus_abys 506 -------------TTGAATTAGATGGTTTAATAGAACCAGGTCATGTATGTACAATAATT Methanococcales-Methanocaldoco 545 ----------------ATTTAGATGCATTTATATGCCCAGGACATGTTTCAACAATCACC Methanococcales-Methanococcus_ 544 ------------CTTAACCTAGATGGATTTATATGTCCTGGCCACGTTTCAACGATTACA delta-Lawsonia_intra 554 -------------GCCAAATTGATGGGTTTCTTTTGCCTGGTCATGTATCTATGGTATTA epsilon-campylobacter_jej 551 -------------TAAAAATCAATGCTTTTTTAGGCCCTTCTCATGTGAGTGTGATAACA epsilon-campylobacter_ups 551 -------------TTAAAATTGACGCTTTTTTGGGACCTTCTCATGTGAGTGTGATTACG clostridia-clostridium_therm 522 --------------AAATATAGACGCTTTTCTCTGCCCCGGTCATGTCAGTGTTATCATC clostridia-carboxidothermus_hy 545 ----------------TAATTGACGGATTTATTTTACCCGGCCATGTTTCAGCAGTAATT Clostr-Halothermothrix_oren 554 -------------TAGATGTAGATGGTTTTATTTTACCTGGGCATGTAAGTACAATTTCT Methanosarcinales-Methanosarci 542 ----------------TAGTTGATGCTTTCATTGCCCCCGGACATGTAGCAACCATCATA Methanosarcinales-Methanosarci 542 ----------------ACGTTGATGCTTTTATAGCTCCTGGGCACGTGGCAACCATAATA Clostridia-Thermoanaerobacter_ 536 ----------------ATGTTCAGGGATTTTTACTGCCGGGACATGTGAGTGCAATAACA clostridia-clostridium_cell 554 -------------TTCAAATTGACGGATTTCTGTATCCGGGACATGTTTCTGCTATAATT cyano-crocosphaera_wat 552 ------------TGTGGTGCAAGGA--TTTTTAGCAGCAGGTCATGTCTGTACCGTTATG Bacteroides_Flavobacteriu_john 547 ------------TGCACCATAAATGCTTTTTTAGGTGCGGGGCATGTCTGTACAATTGTA

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Proteo-others-MagentococcusMC1 589 GGTTCGGAGTGTTGGCGTTTTGCGGT-GAC-CCAGTATGGCATTCCCGTTTCCATTAGTG beta-Nitrospira_multi 583 GGGCCGGAGGAGTGGAATTTCGTCTT-CGA-AAAGCATGACATTCCCACTGCCGTTGCCG beta-Thiobacillus_denitri 580 GGGCCCGAGGAATGGAACTTCGTCGT-CCA-ACGCCACGAGATCCCCGCCGCGGTCGCCG alpha-Acidiphilum 589 GGTGCCGAGGAATGGCGCTTCGTCGC-CGA-GCGTCACGCCCTGCCGGTCGCGGTCGCCG Thermococcales-Pyrococcus_fur 610 GGGGTGAAAGGATGGGCGTATCTCAC-AGA-AAAGTTTGGAATTCCTCAAGTTGTGGCTG Thermococcales-Pyrococcus_hori 610 GGAGTGAAGGGATGGGTTTACTTAAC-GGA-AAAGTTTGGAATTCCTCAGGTTGTTGCCG Thermococcales-Thermococcus_ko 622 GGAGTGAAGGGCTGGGAGTACCTGAC-AGA-AAAGTATGGTATCCCTCAGGTCGTCGCGG Archaeoglobales-Archaeoglobus 604 GGCGTGAAACCGTACAGGGAGATTCT-GAA-GAAATACGGAATACCGCAGGTGATTGCAG Methanopyrales-Methanopyrus_ka 565 GGGACGGGGCCCTACGAGTCCGTAGC-CCG-GGAGC------TCCCGTGCGTGGTCGCCG Methanomicrobiales-Methanospir 547 GGTCTTGAGGGTTATCGAAGGTTTCC-GG-------------TCCCCCAGGTGGTAGCGG Sulfolobales-Metallosphaera_se 607 GGACTTA-GACCTTATTACGAGTATT-TCCTGAGATCTAAAAAACCCATGGTAGCTACTG Thermoproteales-Pyrobaculum_is 658 GGGGGCAAGGCCTGGGCCCCCGTGGC-TGA-GCAATACCGTGTACCTGTAGTTGTGGCGG Methanobacteriales-Methanother 556 GGCATGAAACCCTATGAGCCCTTCTC-AAG-GGACTACAACATACCACAGGTCATAGCGG Methanomicrobiales-Methanocull 549 -GGGCTACGA----GGAGTACG-------A-GCAGTTCCCCGTCCCGCAGGTCGTCGCGG Thermoproteales-Thermofilum_pe 682 GGGGCGAAGGCGTGGGAGCCCGTCGC-GGA-GCAGTTCCAGGTGCCCGTGGTCGTGTCGG delta-Anaeromyxobacter 610 GGCTGGGCGATCTTCGAGCCGTTCGC-GGC-CCGCACCGGCGCGCCGGTGGTGGTGGCCG delta-desulfovibrio_des 601 GGCACGGCCCCGTACAACTTTGTGGC-CGA-ACAGTTTCACACCCCCGGCGTGGTGGCCG delta-geobacter_metalli 607 GGGCCGGAGATCTACCGCTTCCTGGC-CGA-GGAGTTTCATATCCCCTGCGTCATCACCG delta-Pelobacter_propion 607 GGCGCCAACGCCTTCCGCGGACTGGC-AGA-GCAGCGCGGCGTTCCCTGCGTTGTGACCG delta-geobacterFRC32 607 GGTACCGATGCCTACCTCCCACTTGC-GGA-AAGACACCATATCCCCTGCGTGGTGACCG proteo-others-Mariprofundus_fe 607 GGCAGCCGCGCATATG--CCGACTGCTGCGATAAATACCGGGTACCGCTGGTGATTGCCG alpha-Stappia_agg 625 GGCTGCACGCCTTATGAACCGGCGGC-GCGG-GACTTTCGAAAGCCGGTCGTCATTGCCG beta-Methylibium_petro 625 GGTTCCGGACCCTACGAGCACTTCGC--ACAGGAGTACCGCAAGCCGGTGGTCATCGCCG beta-Ralstonia_metall 625 GGCAGTCGGCCCTACGAATTCTTCGC--CGAGGAGTACCGCAAGCCGGTGGTGATCGCCG beta-Rhodoferax_ferri 625 GGCAGTCGGCCGTATGAATTTTTTGC--CGAGGAATACCGCAAACCGGTGGTGATTGCCG beta-dechloromonas_aro 625 GGCAGTCGGCCCTACGAGTTCTTCGC--CGAGGAATACCGCAAGCCGGTGGTCATTGCCG beta-Azoarcus 628 GGCAGCCGGCCCTACGACTTCGTCGC--CACGCAGTACAACAAGCCGGTGGTGATCGCCG beta-Burkholderia-viet 622 GGCTCGGCGCCCTACGAGGGCTTCGC--GCGCGACTATCGGAAGCCGGTCGTGATCGCGG alpha-Magnetospirillum_mag 625 GGCTCGCGACCCTACGAATACTTCGC--CGAGGAATACCAGCGCCCGGTGGTGATCGCCG alpha-Bradyrhizobium_jap 634 GGTACGCAGCCTTACGAGCCCATGGC--GGAACAATTCGAGAAGCCGATCGTGGTCGCCG alpha-BradyrhizobiumBTAi1 610 GGCACCGAGCCTTACGCGCCATTCGC--GCGCGACGATCGCAAGCCGGTGGTGATCGCCG alpha-Rhodopseudomonas_palBisA 625 GGCGCCGATGCGTTCGCGGAATTCGC--CGCAGAGTTCAAGAAGCCGGTGGTGGTCGCGG alpha-Rhodopseudomonas_palCGA0 625 GGCGCCGATGCGTTCGCCGAGTTCGC--GCGCGATTTTGCCAAGCCGGTGGTGGTCGCCG beta-Burkholderia_phy 607 GGCATAGCACCTTATCGCGTGATCGC--GCGTGACTATCGCAAGCCTGTGGTCATCACCG alpha-Rhodospirillum_rub11170 637 GGCATGGCGCCCTTCGACCGCTTCGC--CCAAAGCAACGGCCGGCCGATCGCCGTGGCCG beta-Burkholderia_xeno 628 GGAACCCGTCCGTATCGCTTCATTGC--GGAACAGTACCGGAGGCCGATTACCGTCGCCG alpha-Sphingopyxis_alask 619 GGCACTGATCCCTATGACTTCATCGC--ACGCGACTACCACCGTCCGCTGGTGGTCGCCG gamma-Shigella_flex 619 GGCACTGATGCCTATAATTTTATCGC--CAGCGATTTTCATCGTCCGCTGGTGGTCGCTG gamma-Shigella_sonn 619 GGCACTGATGCCTATAATTTTATCGC--CAGCGATTTTCATCGTCCGCTGGTGGTCGCTG gamma-Shigela_dys 619 GGCACTGATGCCTACAATTTTATCGC--CAGCGATTTTCAGCGTCCGCTGGTGGTCGCTG gamma-EcoliK12 619 GGCACCGACGCCTATAATTTTATCGC--CAGCGATTTTCATCGTCCGCTGGTGGTTGCTG gamma-Ecoli_CFT073 619 GGCACTGATGCCTATAATTTTATCGC--CAGCGATTTTCATCGTCCGCTGGTGGTCGCTG gamma-salmonella_ent 619 GGCACCGAGGCGTACCAGTTTATCGC--CGCCGATTTTCATCGCCCGCTGGTGGTGGCTG gamma-salmonella_LT2 616 GGCACCGAGGCGTACCAGTTTATCGC--CGCCGATTTTCATCGCCCGCTGGTGGTGGCTG gamma-enterobacter_638 619 GGTACAGAGGCTTACAGTTTTATTGC--AGCGGACTATCATCGGCCATTAGTGGTCGCTG gamma-erwinia_caro 619 GGGGTTGATGCTTACCAGTTTATCGC--CACTGAATTCCAGCGACCGTTGGTCATAGCAG gamma-Yersinia_ber 619 GGCACCACGCCGTATGGCTTTATTTG--CGACCAATTCCACAAGCCATTGGTGGTTACCG gamma-Yersinia_int 619 GGCACCACGCCGTATGGCTTTATTTG--CGACCAATTCCACAAGCCATTGGTGGTTACCG gamma-Yersinia_fred 619 GGCACCACGCCATACCACTTTATCTG--TGACCAATTTAATAAGCCGCTGGTGGTCACCG gamma-Yersinia_moll 619 GGCACTTCGCCCTACGATTTTATTTG--CGATCAATTCAATAAACCGCTGGTGGTCACTG gamma-Serratia_protea 619 GGTACCGCGCCGTATCACTTTATCAG--TCGGCAATACCATAAACCGCTGGTGGTGACCG alpha-Rhodobacter_sphaer 619 GGCATCCATGCCTACGATTTCGTGGC--CCGCGACTATTCCAAGCCCCTCGTCGTGGCGG alpha-Rhodobacter_spaer17025 619 GGCACCCACCCCTACGACTTCATCCC--GCGCGATTACGGCAAGCCGATCGTGGTGGCGG alpha-Roseovarius-2601 619 GGCATCCACCCCTATGACTTCATCGC--CCGCGATTACGGCAAGCCCATCGCCGTGGCCG alpha-Paracoccus_deni 619 GGCGTGCATCCCTATGATTTCATTGC--CCGGGACTTCCAGAAACCGCTTGTCGTCGCAG alpha-Xanhobacter_auto 619 GGAACGTCGCCTTACCGCTTCATCGC--CGAGGAGTTCCACAAGCCCCTCGTGGTGGCGG actinobacteria-corynebacterium 598 GGAACCGACACGTTTAAGTTCCTCCC--CGAGGAATTTAACAAGCCCGTTGTCGTCGCAG Actinobacteria-RhodococcusRHA1 601 GGCCTGCGGCCGTACCGGTTCGTGTC--CGAGGTGTACGGCAAACCGATGGTGGTCGCAG Chloroflexi-Acidobacteria 598 GGATATGAAGAGTACCGGCCGCTATC-AGAGAAA-TATCGCGTGCCGATCGTCGTCACCG Chloroflexi-Solibacter_usi 598 GGCTGGAGCGAGTACGAGCCGATCGC-GGCACGC-TATGGGACGCCGATCGTCATCACGG delta-proteo_MMS1 691 GGCTGGCAGGAGTACCAGCCGCTGGT-GGAGCGT-TACCGGGTGCCCATGGTGCCGGCCG Cyan-SynechococcusWH5701 601 GGCAGCCAGGAGTACGTGCCGATCGC-GGCGCGT-TACGGGGTGCCGATCGTGATCAGCG Cyan-Synechocystis6803 598 GGGGAAAGGGCCTATCAACATATCGC-TGAAAAA-TACCAAGTACCCATTGTCATCACTG chloroflexi-chloroflexus_agg 598 GGCATCGAAGAATATCGCTCGCTCGT-CGAAACA-TATCGTGTCCCCATTGTGGTTACCG chloroflexi-chloroflexus_aur 598 GGAACGGATGAGTACCGCCCACTCGT-CGAACAG-TACCGCGTACCGATTGTGGTCACCG Chloroflexi-Roseiflexus_cas 598 GGCACCTGGCAGTACCGGCCGCTGGT-TGAACGG-TACCATGTGCCAATTGTTGTCACCG Chloroflexi-RoseiflexusRS-1 598 GGCACCTGGCAGTACCAGCCGCTCGT-TGAGCGC-TACCGTGTGCCGATTGTGGTGACCG Actinobacteria-Mycobacterium_g 598 GGCACCGACGAATACCCGCCGCTG-T-GCGACAAGTACGGAACACCGATCGTCGTCACGG Actinobacteria-Mycobacterium_v 598 GGCACCGACGAGTACCCGCCGCTG-T-GCGACAAGTACGGAATCCCGATCGTGGTCACGG Actinobacteria-FrankiaCcI3 598 GGCACCGGCGAGTACCCGGCCCTGGC-ACGGCGT-TACGGCGTGCCGATCGTGGTCACCG Actinobacteria-FrankiaACN14a 598 GGCACCGGGCAGTACCCGGAGCTCGC-CCGCCGG-TACCAGGTGCCGATCGTGGTCACCG Actinobacteria-Streptomyces_av 598 GGCGTGGGGGAGTACCCGGAACTGGC-CGAACGC-TTCCGCGTCCCGATCGTCGTGACCG Actinobacteria-Mycobacterium_s 598 GGCACGTCCGAATACGGGCCTCTGGT-GGAGAAG-TTCGACGTCCCGATCGTGGTGACCG Chlorobi-Pelodictyon_lut 598 GGATATGAGCAGTACCTGCCTCTTTC-GGCCGAA-TTCCATGTGCCCATTGTCCCTACCG

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Chlorobi-Pelodicton_lut 598 GGCTACGAGGAATATGAGCCAGTCGC-CCGTGAG-TTTCATGTGCCGATAGTGCCGACCG chlorobi-chlorobium_lim 598 GGTTATGAAGAGTACCGGCAGATAGC-CCGGGAT-TTTCATGTGCCGATCGTTCCTACCG Chlorobi-Prosthecochloris_aest 598 GGGCTTTGCGACTATTTCCCTGTTGC-TGAAACA-TATGGTGTTCCGATTGTTCCCGCGG chlorobi-chlorobium_tep 598 GGGTACGAGGAGTACGAGCCGGTGGC-GGCGGAG-TTCGGCGTGCCGATCGTGCCCGCCG delta-Syntrophus_acid 610 GGCGTCGATGCTTATAAGCGCATCCC----GGAAACAGGACGGGCTG-CCGTGATCACCG delta-desulfotalea_psy 598 GGCTCAGAGGTCTACGAACCCCTGGC--CCGGGACTATAATCTTGCCTGTGTGGTTGCCG chloroflexi-dehalococcoides_et 598 GGCAGCAATGCCTGGGAATTTATTCC--AGAGGAATACCATCTGGCTTGTGCCGTGTCAG chloroflexi-dehalococcoides_CB 598 GGCAGTAACGCCTGGGAGTTTATCCC--CAAAGACTACCATCTGGCCTGTGCCGTTTCCG clostridia-desulfitobacterium_ 607 GGCAGCGAAGCCTTGGCCTTTATGGC--TGAGGATTACGCCATGCCCGGAGTGGTTACTG Clostr-Moorella_therm 619 GGCAGGCGGGCGTTTGACTTTATCGT--TAAAGAGTATGGCCTGCCGTCAGTAATCAGCG Bacteroides-Robiginitalea_bif 598 GGTATGGATGAATATTATCCATTAGT--GGCCCATTACAGAATCCCAATGGTAGTCACTG epsilon-Helicobactr_acin 616 GGCGCTCAAATCTATACCCCTTTAGT--GGAGTGCTTTAATATCCCCATTATTGTGAGCG epsilon-Helicobacter_pylor 616 GGCGCTCAAATCTATGCTCCTTTAGT--GGATCGCTTTAAAATCCCCATTATCGTGAGCG gamma-PhotobacteriumSKA34 622 GGGGCGAAGATTTATCGTCCATTGGT--AGATAAATACAATACACCTGTTGTGGTTGCAG gamma-Vibrio_ang 622 GGGGCGAAGATTTATCGTCCGTTGGT--AGACAAATACAATACACCCGTTGTGGTTGCAG Gamma-Photobacterium_prof 619 GGCGCTAAAATCTACCAGCCCATTAT--CGATAACTACCGTGTGCCGTTGGTTGTAGGTG epsilon-Wolinella_succ 616 GGAGCGAAAATCTATGAGCCCTTGGT--GCAAGACTATCGACTTCCTATTGTGGTGTCGG epsilon-campylobacer_con 598 GGTAGCAAAATTTATAAAGAGCTAGC--GAGCGAATTTAAAAGACCCATCGCCATTAGTG Aquifex 643 GGGACGAAACCTTACCACTACTTTGC--CGAGGAATTCCAGAAACCGGTGGTGATAGCGG gamma-Actinobacillus 622 GGCACCGCTCCCTATCAACCGCTTGC--CGAGCAATATCATAAACCGTTTGTGGTTACCG gamma-Manheimia-succ 622 GGCTCCGCACCTTATAGGGAACTTTG--TAAAAAATTCCGTAAACCTTTTGTAATCGCCG gamma-Acidobacillus_succinogen 622 GGCTCGCAGCCTTATCAAGCGTTGTG--CGATCAATTCCATAAACCTTTTGTCATCACCG Cyano-Avar 607 GGTACTGAACCTTATGAATTTATTGC--CCAAAAATATCATAAACCAATTGTTGTTTCCG Cyan-Nostoc7120 607 GGTACTGAACCTTATGAATTTATTGC--CCAACAATATCATAAGCCAATTGTTGTTTCCG Cyan-Nodularia_spu 607 GGAACAGAACCATATCAATTTATTGC--CCAAAAATATCATAAACCCATAGTAATTGCCG Cyan-Lyngbya8106 619 GGTTCAAATCCCTATCAAATTATCGC--TGAAAAATATTACAAACCTGTCGTTGTTTCCG Cano-trichodesmium_ery 619 GGTACTCAAGTATATCACATAATTTC--GGAAAAATACCAAAAACCTGTGGTTGTATCAG clostridia-caldicelluosiruptor 562 GGAGTAGATGGTTTTAAGTTTGTTGA--AAGG---TTTAAGGTTGCCTCTGTAATTTCGG clostridia-clostridium_beij 580 GGAGCAGATTATTTTAAATTTATTGT--AGAGGAATATAATATTCCAGCAGTAATTGCAG Methanobacteriales-Methanospha 553 GGAACAAAACCATTTGAATTTTTATC--AGAAGATTATGGAATTCCACAAGCAGTAGCAG Thermococcales-Pyrococcus_abys 553 GGAACAAAACCATTTGAATTTTTATC--AGAAGATTATGGAATTCCACAAGCAGTAGCAG Methanococcales-Methanocaldoco 589 GGATTAAAGCCTTATTATGGGTTGTG--TGAAAAATACAAAGCTCCAATGGTTGTTGCTG Methanococcales-Methanococcus_ 592 GGTTTAAAACCATACTACGACCCTTG--TAAGACGTACCATGCACCAATGGTTGTTGCTG delta-Lawsonia_intra 601 GGTTTAGAGCCATATGCATTTGTTGT--TGAGAAGTGGGGGAAGCCTGCTATAGTTGCTG epsilon-campylobacter_jej 598 GGTTATGGTATTTATGAGCCTTTAGC--AGCAAAGTTTAAAACCCCTATAGCGGTGAGTG epsilon-campylobacter_ups 598 GGTTATCAAATTTATGAAAATTTGGT--CAAAACATTTCGAACGCCTATAGCTGTGAGCG clostridia-clostridium_therm 568 GGCTCCTCGGTTTATACCGATATGGC--GTTAAAATACCGCAAGCCTTTTGTGATAGGCG clostridia-carboxidothermus_hy 589 GGAGCCAAAGCTTTTAGATTTATTAC--CGAAAATTATCAAATACCTGCTGCGGTTACGG Clostr-Halothermothrix_oren 601 GGTTTGCAGCCCTTTGAGTTTTTAGC--CAGGGATTACCATGTTCCCGGGGTAGTGGCAG Methanosarcinales-Methanosarci 586 GGGACAAAACCCTTCGAACATCTTGC--AGAAA---AAGGTTTTCCTGTTGTGGTAAGCG Methanosarcinales-Methanosarci 586 GGCACAAAACCTTTTGAGTCTCTTGC--AGAAA---AAGGCTTTCCTATTGCCGTAAGTG Clostridia-Thermoanaerobacter_ 580 GGAAGCCGCATATATGAGTTTTTGCC--AAGAGAGTTTGGAGTAGGTGGTGTGATATCAG clostridia-clostridium_cell 601 GGTACAGACTTTTTTTCCTATGTGGC--GTATAATATGGAAAAGGCTGGTGTGGTGGCAG cyano-crocosphaera_wat 598 GGCTACGAAGCTTATAAACCTATCGC--TCAAAAATATAAAGTACATATCGTCATAACAG Bacteroides_Flavobacteriu_john 595 GGTCTGGAAGAATATTATCCTATTGC--CGAAAAATACAAAATACCAATCGTCGTTTCCG

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Proteo-others-MagentococcusMC1 647 GCTTTTCTGACCGTAGCTTT-TTGGCTTCACTGGATACGGTGGTGCGCCAAGTTGTGTCG beta-Nitrospira_multi 641 GCTTC-CAGCCCGTGTCACTGCTAGCCGCCATGTATTCCGTATTACGCCAACTGCTTGAA beta-Thiobacillus_denitri 638 GGTTC-GGCACGGCCTCGCTGCTCGCCTCCTTCTATTCGGTGCTGCGCCAGCTCGAGAGC alpha-Acidiphilum 647 GGTTCTCCGCCGAAAGC-CTGCTGGCGGCGATCTATTCGGTCCTCCGCCAGAAGCGCGAG Thermococcales-Pyrococcus_fur 668 GCTTT-GAGCCAGTTGATGTTTTACTCGGAATACTTATTCTCATTAGGCTTGTGAAGAGG Thermococcales-Pyrococcus_hori 668 GCTTT-GAGCCGGTAGATGTACTTTTAGCGATACTAACCTTAATTCGGCTTGTGAAGAGA Thermococcales-Thermococcus_ko 680 GCTTC-GAGCCGAACGACGTCCTCATGGCGATTCTTATGCTCATCAGGATGTACAAGGAA Archaeoglobales-Archaeoglobus 662 GATTC-GAGCCTGAAGATGTTATGCTTGCCATCTATCTTCTCCTTGAGGCGATGAGTGAT Methanopyrales-Methanopyrus_ka 617 GCTTC-GAGCCCGAGGACGTTCTGATCGCGGTACTGGCGTGCCTCAAGATGCTCAGAAGG Methanomicrobiales-Methanospir 593 GTTTT-GAGCCTGATGATATTCTGCTTGGTCTTCTTATGCTGGTACAGCAGATCAGGGAA Sulfolobales-Metallosphaera_se 665 GGTTT-GAACCCATAGATGTACTTATGTCCATTTACATGATTCTAAAGCAAATTGATGAG Thermoproteales-Pyrobaculum_is 716 GGTTT-GAGCCTAACGACGTCCTTACGGCAATTGCCGAAATTTTAAGACAGTTAAAAAAT Methanobacteriales-Methanother 614 GCTTC-AACCCCCTGGATATACTCATGGCGGTCTACATGATACTCCGACAGATCGATAGG Methanomicrobiales-Methanocull 596 GGTTC-GAGCCGGAGGACATCCTCCTCGCCCTCCTGATGGCGGTCAGGCAGGTCAAGGAG Thermoproteales-Thermofilum_pe 740 GGTTC-GAGCCTATAGACGTGCTCATGGGTATCGCGGAGATCATGCGGCAACTGGCGAGG delta-Anaeromyxobacter 668 GGTTC-GAGCCGCTCGACATCCTGGCCGCGCTGGTGAAGCTCACCGAGCTCATCCGCGAG delta-desulfovibrio_des 659 GTTTT-GACCCTGCCGACATTCTGCAGGCGCTGCTGCTGATGGTGCAGATGCGCCGCCGA delta-geobacter_metalli 665 GTTTC-GAGCCTGCGGACGTCATGCAGGGGGTCGAGATGCTCGTCCGCCAGGTGGTGGCG delta-Pelobacter_propion 665 GTTTC-GAGCCGGCCGATGTCATGCAGGGAGTGGAGATGCTGGTACGGCAGGTGGTGGAG delta-geobacterFRC32 665 GTTTC-GAACCGGCGGATATCATGCAGGGGGTGGAGATGCTGATCCGGCAGAAGATTGCC proteo-others-Mariprofundus_fe 665 GTTTT-GAACCGGTCGATGTCATCCAATCGGTACTGATGCTGGTGCGACAGATCAATGCC alpha-Stappia_agg 683 GGTTC-GAGCCGCTCGACGTGATGCAGTCGACGTTGATGCTGATCCGCCAGATCAACGAA beta-Methylibium_petro 683 GCTTC-GAGCCGCTCGACGTGATGCAGGCGGTGCTGATGCTCGTGCGCCAGGTGAACGAA beta-Ralstonia_metall 683 GCTTC-GAGCCGCTGGACGTGATGCAGGCCATCCTCATGCTGGTGACGCAGGTCAACGAG beta-Rhodoferax_ferri 683 GCTTT-GAGCCGCTGGACGTGATGCAGGCGATTCTGATGCTGGTCAAGCAGGTCAACGAA beta-dechloromonas_aro 683 GCTTC-GAACCGCTCGACGTGATGCAGGCGATCAAGATGCTGATCCGCCAGGTCAACGAA beta-Azoarcus 686 GCTTC-GAGCCGCTCGACGTGATGCAGTCCATCCTGATGCTGGTGCGCCAGGTGAATGAC beta-Burkholderia-viet 680 GCTTC-GAGCCGCTCGACGTGATGCAGGCGATCCTGATGCTGATCCGCCAGGTCAACGAC alpha-Magnetospirillum_mag 683 GCTTC-GAGCCGCTGGACGTCATGCAGGCGGTGCGCATGCTGGTCCGCCAGATCAACGAC alpha-Bradyrhizobium_jap 692 GATTT-GAACCCCTCGACGTGCTGCAGGCTGTCCTGATGCTGGTGCGGCAAGTGAACCAA alpha-BradyrhizobiumBTAi1 668 GCTTC-GAGCCGCTCGACATGATGCAGGCGATCCTGATGCTGATCGAGCAGGTCAATGAC alpha-Rhodopseudomonas_palBisA 683 GCTTC-GAGCCGCTCGACGTGATGCAGTCAATCCTGATGCTGATCCATCAGGTCAATGAC alpha-Rhodopseudomonas_palCGA0 683 GCTTC-GAACCGCTCGATGTGATGCAGTCGATCCTGATGCTGGTGCAGCAGGTCAATGAC beta-Burkholderia_phy 665 GTTTC-GAACCGCTCGACGTGCTGCAAGCCGTGCTCATGCTCATCACACAGATCAACGAA alpha-Rhodospirillum_rub11170 695 GTTTT-GAGCCCACCGATCTGCTGCAAGCCGTGCTCGCCCTGGTGACTTTGACCAACGAG beta-Burkholderia_xeno 686 GATTC-GAGCCGCTCGATGTGCTGCATTCGGTCTTCATGGTCTTGCGCCAGATCGCAGAA alpha-Sphingopyxis_alask 677 GCTTC-GAACCGCTCGACGTATTGCATTCGGTTTGGATGGTGCTTCGGCAGATGGCCGAA gamma-Shigella_flex 677 GTTTC-GAACCCCTTGATCTACTGCAAGGCGTGGTCATGCTGGTGGAGCAGAAAATAGCG gamma-Shigella_sonn 677 GTTTC-GAACCCCTTGATCTACTGCAAGGCGTGGTCATGCTGGTGGAGCAGAAAATAGCG gamma-Shigela_dys 677 GTTTC-GAACCCCTTGATCTACTGCAAGGCGTGGTGGTGCTGGTGGAGCAGAAAATAGCG gamma-EcoliK12 677 GATTC-GAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCG gamma-Ecoli_CFT073 677 GTTTC-GAACCCCTTGATCTACTTCAAGGCGTGGTGATGCTGGTGGAGCAGAAAATAGCG gamma-salmonella_ent 677 GATTC-GAACCGCTTGATCTACTGCAAGGCGTGGTCATGCTGGTTGAGCAGAAAATAGCG gamma-salmonella_LT2 674 GATTC-GAACCGCTTGATCTACTGCAAGGCGTGGTCATGCTGGTTGAGCAGAAAATAGCG gamma-enterobacter_638 677 GTTTC-GAACCACTTGATCTACTGCAAGGCGTCAACATGCTGGTTGAGCAGAAAATAGCA gamma-erwinia_caro 677 GATTT-GAACCGGTTGACCTGCTGCAAAGTGTTCTCATGTTGATCGAGCAAAAACTCGCC gamma-Yersinia_ber 677 GTTTT-GAGCCGCTGGATATCTTGCAGGGGCTGGTAATGCTGCTTGAACAGATGGTCAGC gamma-Yersinia_int 677 GTTTT-GAGCCGCTGGATATCTTGCAGGGGCTGGTAATGCTGCTTGAACAGATGGTCAGC gamma-Yersinia_fred 677 GCTTT-GAGCCACTGGATATTTTACAAGGGCTGGTGATGTTGCTGGAGCAGATGGTCAGT gamma-Yersinia_moll 677 GCTTC-GAACCGCTGGATATCCTGCAAGGGCTGGTAATGCTGCTGGAGCAAATGGTCAGC gamma-Serratia_protea 677 GCTTT-GAACCGCTGGATATCCTGCAAGGGGTGATGATGCTGGTTGAGCAGTTTGTGGGG alpha-Rhodobacter_sphaer 677 GCTTC-GAGCCCACGGACCTCCTGCAATCGGTGCTGATGGTGCTGGACCAGATCGCCGAG alpha-Rhodobacter_spaer17025 677 GGTTC-GAGCCGACCGACCTGCTGCAATCGGTCCTGATGGTGCTGACCCAGATCGCCGAG alpha-Roseovarius-2601 677 GGTTC-GAGCCGCTCGACCTGCTGCAATCGGTGCTCATGGTGCTCGAGCAGATCCGCGAC alpha-Paracoccus_deni 677 GTTTC-GAGCCGACCGATCTTCTGCAATCGGTGCTGATTGTGCTGGACCAGATCGCCGAG alpha-Xanhobacter_auto 677 GCTTC-GAGCCCATCGACCTGTTGCAATCCCTGCTGATGGTGCTGCGCCAGATCGAGGCC actinobacteria-corynebacterium 656 GATTC-GAGCCTCTCGACGTTCTTCAAACCGTGGACATGCTGCTCCAGCAGTTCCTCCGC Actinobacteria-RhodococcusRHA1 659 GCTTC-GAGCCCCTCGACATCCTCGCGTCGGTGCACATGCTGCTCCAGCAGATCCGGGAG Chloroflexi-Acidobacteria 656 GCTTT-GAGCCACTCGACATTCTCCAGGGCGTGTTGATGTGCGTACGGCAGCTTGAGAAC Chloroflexi-Solibacter_usi 656 GCTTC-GAACCTGTGGACCTGCTGGAAGGCATTCTGCTGACGGTGCGGCAGTTGGAGCAG delta-proteo_MMS1 749 GTTTC-GAGCCGGTGGATATCCTGGCCGCCATCCTGATGCTGGTGCGGCAGTTGGAGGCG Cyan-SynechococcusWH5701 659 GCTTC-GAACCGCACGACCTGCTGGAGGGGATGCTGCGAGCCGTACGGCAGCTGGAGCAC Cyan-Synechocystis6803 656 GCTTT-GAACCTGTGGATATTATGCAGGGCATCTTTGCCTGTGTGCGCCAACTGGAGTCG chloroflexi-chloroflexus_agg 656 GTTTT-GAGCCACTCGACGTACTCGAAGGCATTCGTCGCGCCATTCTCCAACTCGAGCAA chloroflexi-chloroflexus_aur 656 GCTTC-GAGCCACTCGATGTGTTAGAGGGTATTCGCCGCGTCATTCTTCAGCTCGAACAG Chloroflexi-Roseiflexus_cas 656 GTTTC-GAGCCGCTCGACGTACTGGAAGGGATCCGCCGCGTCGTTCTGCAACTGGAAGCG Chloroflexi-RoseiflexusRS-1 656 GTTTT-GAACCGCTCGATGTGCTGGAAGGCATTCGCCGCGTCATTCTGCAACTCGAATCG Actinobacteria-Mycobacterium_g 656 GTTTC-GAACCGCTCGACATCCTGGAAGGCATCCGGCGCACCGTGATTCAACTGGAGGCC Actinobacteria-Mycobacterium_v 656 GATTC-GAGCCGCTCGACATCCTGGAGGGCATCCGGCGCACGGTCGTGCAGTTGGAGTCC Actinobacteria-FrankiaCcI3 656 GCTTC-GAACCACTCGACCTCCTCGAGGGCATCCGCCGCACCGTGGTGCAGCTGGAGGCC Actinobacteria-FrankiaACN14a 656 GCTTC-GAGCCCCTCGACCTGCTCGAGGGCATCCGCCGGACCGTGACGCAACTGGAGGAA Actinobacteria-Streptomyces_av 656 GGTTC-GAGCCACTGGACATCCTCGAAGGCGTACGCCGCACCGTTCTCCAGCTGGAACGG Actinobacteria-Mycobacterium_s 656 GATTC-GAGCCGCTGGACCTGCTCGAGGGGGTGCGGCAACTCGTGGACCTGTTGGAGGCC Chlorobi-Pelodictyon_lut 656 GATTC-GAGCCGGTTGACCTGCTTGCCGGGATACTGAAAACGGTTGAACTGCTTGAGGAC

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Chlorobi-Pelodicton_lut 656 GTTTT-GAGCCGGTTGACCTCCTTGCCGGAATACTGAAAACGGTCGAACTGCTTGAAGAG chlorobi-chlorobium_lim 656 GTTTT-GAGCCGGTGGATCTGCTGGCCGGAATTCTTGAAACGGTCGGATTGCTCGAAGAT Chlorobi-Prosthecochloris_aest 656 GGTTC-GAACCTGTCGATCTCCTGAACGCTGTTTTGAAGGTTGTCGAGATGCTTGAAGCC chlorobi-chlorobium_tep 656 GGTTC-GAGCCGGTCGATCTGCTCGACGCGATTCTGAAGACGGTCGAGCTCCTCGAAGCT delta-Syntrophus_acid 665 GTTTT-GAACCGGTGGACATTCTCGAAGGCATCCTCATGATCCTGCGCCAGCTTCAGGAA delta-desulfotalea_psy 656 GTTTT-GAAACCGCTGACATCCTTGGCAGCCTCATCCAACTGGCCAGACAGGTAAACGCA chloroflexi-dehalococcoides_et 656 GCTTT-GAAAGCCTGGACATACTCTACTGTGTTAAGTCTCTGGTAGACCAGGTGGAAGAC chloroflexi-dehalococcoides_CB 656 GTTTT-GAAAGCCTGGACATACTCTACTGTGTCAAGTCACTGGTAGACCAAGTGGAGGAC clostridia-desulfitobacterium_ 665 GTTTT-GAAGCTCTGGATATCCTGGAGGGGTTGGTCATGCTGCTCCGGCAAAGAGACCAA Clostr-Moorella_therm 677 GCTTT-GACCCCATGGATATTCTGATTGCCCTTCACGAGCTTTTGCTTCAATTGCTCCGG Bacteroides-Robiginitalea_bif 656 GTTTT-GAACCTGTGGACCTTTTGCAGGGTATACTACTTACCGTTCAACAATTGGAAAAG epsilon-Helicobactr_acin 674 GTTTT-GAGCCGGTGGATATATTAGAAAGCGTGTTAATGCTACTAAAACAAACCCTAAAC epsilon-Helicobacter_pylor 674 GTTTT-GAGCCGGTGGATATATTAGAAAGCGTGCTGATGCTTATCAAACAAGCCCTAAAC gamma-PhotobacteriumSKA34 680 GTTTT-GAGCCCGTTGATGTCATGGAATCTATTCTACGTATCACTAAACAAAAAATCGCA gamma-Vibrio_ang 680 GTTTT-GAGCCTGTTGATGTGATGGAATCTATTTTACGTATCACTAAACAAAAAATCGCT Gamma-Photobacterium_prof 677 GTTTT-GAACCTGTCGATGTCTTGGAGTCGGTTTTGATGCTGGTAGAACAGGCAGTATCT epsilon-Wolinella_succ 674 GTTTT-GAACCTGTGGATGTGATGGAGAGCGTGGATAGAATCGTCCACCAGCTGGCTCAC epsilon-campylobacer_con 656 GTTTT-GAGCCGCTTGACATCATGGCAAGTGTGCTAAATTTAGTTCGTCAGCAAAACGCA Aquifex 701 GGTTT-GAACCATTAAACGTCCTGAAGGCCGTTTACCTAATACTGAAACAAATTTACAAG gamma-Actinobacillus 680 GCTTT-GAGCCTTTAGATTTATTACAAGCGATTGTGATGCTAGTCGAACAATTCGTCGAG gamma-Manheimia-succ 680 GGTTT-GAACCTTTAGATATTCTGCAATCTATTGTTATGTTGGTAGAGCAGTTTGCGGAC gamma-Acidobacillus_succinogen 680 GATTT-GAACCGCTGGATATTCTGCAGGCTATCGTGATGCTGGTGCAGCAATTTGCGGAC Cyano-Avar 665 GCTTT-GAACCATTAGATATTCTCCAATCAATTTGGATGTTGTTACAGCAACTAAGAGAA Cyan-Nostoc7120 665 GTTTT-GAACCGTTAGATATTTTCCAATCCATTTGGATGTTGTTACAACAACTAGTAGAA Cyan-Nodularia_spu 665 GCTTT-GAACCATTAGATATACTGCAATCAATTTGGATGATATTACAGCAACTAGTAGAA Cyan-Lyngbya8106 677 GCTTT-GAACCTTTAGATATTGTGCAGTCAGTATGGATGTTACTGCAACAGTTAGTAGAC Cano-trichodesmium_ery 677 GTTTT-GAACCTTTGGATATTTTGCAGTCTATTTGGATGTTACTACAGCAAATATTAGAA clostridia-caldicelluosiruptor 617 GGTTT-GAAGGTTATGATAT-------ACTTCTTTCACTTTTAAGTATTACCCAGGACAT clostridia-clostridium_beij 638 GTTTT-GAAGCTTTAGATATTGCTGGTGCTTTATATTTTTTGACGAAACAACAAAATAAA Methanobacteriales-Methanospha 611 GATTT-AATCCATTAGATATCTTGTATTCAATATACCTTATATTAAAACAGAAAAAAGAA Thermococcales-Pyrococcus_abys 611 GATTT-AATCCATTAGATATCTTGTATTCAATATACCTTATATTAAAACAGAAAAAAGAA Methanococcales-Methanocaldoco 647 GCTTT-GAGCCAATAGATGTGTTAATGGCTATAATAATGATTTTAAAGCAAGTCATCAGT Methanococcales-Methanococcus_ 650 GCTTT-GAACCAATTGATGTAATGGCTTCAATCGTGATGATTTTAAAACAGCTCGTAAAT delta-Lawsonia_intra 659 GTTTT-GAACCAGCGGATATTTTAGATGCGTTATGCCGTATGGCAAAACAACTACAAACA epsilon-campylobacter_jej 656 GTTTT-GAGCCAGTTGATATTTTAGAAAGTGTGTTAAATATCATAAAACAAAGCAATGAA epsilon-campylobacter_ups 656 GTTTT-GAGCCTGTGGATATTATGGAAAGTGTGCTAAATATCATCTTGCAAAAAAACGCC clostridia-clostridium_therm 626 GTTTC-GAGGGTGGGCATATTATAGCGGCAATTTATGAAATAATCCGCCAGATTTCAAAC clostridia-carboxidothermus_hy 647 GATTT-ACCGCCGAAGAGCTTCTTTTAGGAATAAGTTTGGTAGTTAAATCAATTTTAAAA Clostr-Halothermothrix_oren 659 GTTTT-GAGCCAGGTGATATTATTTTGTCAATATATACTCTGTGTAAAATGTTAAAAGAT Methanosarcinales-Methanosarci 641 GGTTT-GAGGCAAGGGATATCCTCCTTGGGATAAATCTTCTCCTGACCCAGACGGAACAG Methanosarcinales-Methanosarci 641 GGTTT-GAACCAGGAGATATTCTCCTTGGAATAAACCTGTTACAACTGCAGGCAGAAGAA Clostridia-Thermoanaerobacter_ 638 GTTTT-GAGGCAGAAGATATTTT------GATGAGCATAGTAATGCTTTTAAAAAATTAT clostridia-clostridium_cell 659 GCTTC-GAACCATTGGATATACTGTATTCCATTAAGGTGCTAGTAGATAATATTGCAAAC cyano-crocosphaera_wat 656 GGTTT-GAACCTATCGACGTTTTGCAAGGAATTTATCATTGTATTCAACAATTAGAAACC Bacteroides_Flavobacteriu_john 653 GTTTT-GAACCTGCTGATATGGTGCAGGCGATTTATCATACCGTTTTGCAATTGGAACAA

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Proteo-others-MagentococcusMC1 706 GGTAAACCCCAATTGGACATTCAAGAACCCTATCG-TATTAACCCCCGTGGCAACCTCTA beta-Nitrospira_multi 700 GGGAAGCGTTTTCTGGATAATTGTTATCCTGAGTT-AGTGCGGCCCGGGGGAAATCGAGC beta-Thiobacillus_denitri 697 GGTGAACGCTTTCTCGACAACTGCTACCGCGAAGT-CGCGCACCCGGACGGGAATCCAAC alpha-Acidiphilum 706 GGCAGGGCCTTCCTCGACAATTGCTATCCCGAGCT-GGTCCGCCCGGAGGGCAACCCCAC Thermococcales-Pyrococcus_fur 727 GGCGAAGCGAAAATAATCAACGAGTATAATAGAGT-TGTAAAGTGGGAAGGAAATGTCAA Thermococcales-Pyrococcus_hori 727 GGGGAAGCTAAGATTGTTAATGAATACAATAGAGC-GGTAAAGTGGGAAGGAAACGTTAA Thermococcales-Thermococcus_ko 739 GGCGAGGCGAGGATAATCAACGAGTACGAGCGGGC-TGTCAAGTACGAAGGCAACGTGGT Archaeoglobales-Archaeoglobus 721 GGCAGAAGGGAGGTTATCAACGAGTACAGCAGAGC-GGTAAAGGAGGAGGGAAACGTCAG Methanopyrales-Methanopyrus_ka 676 GGACGTGTGGGAGTCACCAACGAGTACCTCAGGGC-AGTCGAGGACAGGGGGAACCCGGT Methanomicrobiales-Methanospir 652 GGCGTCGCACGGGTCGATAATGCGTATCCACGAGC-GGTTTCTGATGAGGGAAATCCGAA Sulfolobales-Metallosphaera_se 724 GGTAAACCAAAGATAGAGAATGAATACACCAGATC-AGTAACTTGGGAAGGTAATGTGAA Thermoproteales-Pyrobaculum_is 775 GGCGAACATAAAGTCGTAATTGAGTATGTAAGAGC-CGTAAAGTGGGAGGGAGATTTAAA Methanobacteriales-Methanother 673 GGAGAGGCAAAGGTTGAGAACGAGTATAAGAGGGC-CGTGAAACCCGAGGGTAACCTCAA Methanomicrobiales-Methanocull 655 GGGGCGCACCGGGTGGAAAACGCCTACCCGCGCGT-GGTCACCCGGGAAGGGAACGTCAA Thermoproteales-Thermofilum_pe 799 GGGGAGTACGGGGTCAAAGTGGAGTATACGCGGGC-GGTGACGTGGGAGGGGGACCTGGA delta-Anaeromyxobacter 727 GGCCGGCCGGAGGTGGCGAACGTCTACCCGCGCTG-CGTGACGAAGGAGGGCAACCTCCC delta-desulfovibrio_des 718 GGCGAATACGCCATCACCAACCAGTACAGCCGCGC-GGTAAGCGACAACGGCAACCCCAA delta-geobacter_metalli 724 GGGGAGAGCCGGGTGGAGACCCAGTACAGCAGGGT-GGTACGGCCTGAGGGGAACCGCAA delta-Pelobacter_propion 724 GGGAAAAGCCGGGTGGAAAACCAGTACAGCAGGGT-GGTGACCTGGGAAGGTAACTGCAA delta-geobacterFRC32 724 GGGGAGGCGAATGTGGAGAACCAATACCGCCGGGT-GGTGAAACGGGACGGGAACAGCAA proteo-others-Mariprofundus_fe 724 GGAGAGGCAGAAGTGGAGAACCAGTATACCCGCGC-AGTCAGCCAAACCGGCAATCAAAA alpha-Stappia_agg 742 GGTCGACACGAAGTGGAGAACCAGTACACCCGCGT-TGTGACCAGGGAAGGCAATCGCAA beta-Methylibium_petro 742 GGGCGCGCCGAGGTCGAGAACGAGTTCACCCGTGC-GGTCAACCGCGACGGCAACCTGAA beta-Ralstonia_metall 742 GGCAGAGCCGAGGTGGAGAATGAATTCACTCGCGC-CGTCACCCGAGACGGCAACCAGAA beta-Rhodoferax_ferri 742 GGGCGTGCCGAGGTTGAAAACGAGTTCGCACGCGC-GGTCAGCCGCGACGGCAACCAGAA beta-dechloromonas_aro 742 GGGCGCGCCGAGGTCGAGAACGAATTCGCCCGCGC-CGTCAGCCGCGACGGCAACCTCAA beta-Azoarcus 745 GGCCGCGCCGCGGTCGAGAACGAATTCACCCGCGC-CGTCACCCCGGACGGCAACCGCAA beta-Burkholderia-viet 739 GGCCGCGCCGAGGTCGAGAACGAATTTTCGCGGGC-GGTCACGCGTGCCGGCAACGCCGC alpha-Magnetospirillum_mag 742 GGCCGCTTCGAGGTGGAGAACGAGTTCGCCCGCGC-CGTGACTCGCGACGGCAACGAGAA alpha-Bradyrhizobium_jap 751 AACCGCCACGAGGTGGAAAACCAATACAGCCGTGC-AGTGACACGCGAAGGCAACCGGCG alpha-BradyrhizobiumBTAi1 727 GGCCGCCACGAGGTCGAGAACCAGTATCGCCGCGC-GGTGGCCGGTGGCGGCAACCAGCG alpha-Rhodopseudomonas_palBisA 742 GGCCGCCACACGGTCGAGAACCAGTATGTCCGCGC-GGTGACGGGGCAAGGCAACCGGAT alpha-Rhodopseudomonas_palCGA0 742 GGCCGCCACGTCGTCGAGAACCAGTACATCCGCGC-GGTGACGCCGGGGGGCAACCGCAT beta-Burkholderia_phy 724 GACCGCACGGATGCTGAGAACCAGTACACACGTGC-TGTCACCGAACAGGGCAACCTGAA alpha-Rhodospirillum_rub11170 754 GGGGCGGCGCGCGCCGTCAACCGCTATACCCGGGC-GGTGACCGAGGCCGGCAACACCAC beta-Burkholderia_xeno 745 GGCCGCAGCGAGGTGGAAAACCAGTATGCCCGTAT-CGTGCCCGAGAATGGCAATGCACA alpha-Sphingopyxis_alask 736 GGCCGGGCGGAGATCGAAAACCAGTATGACCGGAT-TGTGCCACGCCATGGCAATGAAGC gamma-Shigella_flex 736 GCCCACAGCAAGGTAGAGAATCAGTATCGTCGGGT-GGTACCGGATGCCGGTAACCTACT gamma-Shigella_sonn 736 GCCCACAGCAAGGTAGAGAATCAGTATCGTCGGGT-GGTACCGGATGCCGGTAACCTGCT gamma-Shigela_dys 736 GCCCATAGCAAGGTAGAGAATCAGTATCGTCGGGT-AGTGCCGGATGCCGGTAACCTGCT gamma-EcoliK12 736 GCCCACAGCAAGGTAGAGAATCAGTATCGTCGAGT-GGTACCGGATGCCGGTAACCTGCT gamma-Ecoli_CFT073 736 GCCCACAGCAAGGTAGAGAATCAGTATCGTCGGGT-GGTACCGGATGCCGGTAACCTGCT gamma-salmonella_ent 736 GCCCTAAGCCAGGTTGAAAACCAATACCGTCGCGT-GGTGCCGGATGCCGGAAACATGCT gamma-salmonella_LT2 733 GCCCTAAGCCAGGTTGAAAATCAATACCGTCGCGT-GGTGCCGGATGCCGGAAACATGCT gamma-enterobacter_638 736 GCCCTGAGCGTGGTCGAAAATCAGTATCGCCGCGT-CGTGCCGGATGCGGGAAATCCGCT gamma-erwinia_caro 736 GGTCAACCTGATATTGAAAATCAGTACCGCCGTGT-GGTGCCAGATGCAGGCAACACGCT gamma-Yersinia_ber 736 GGGCGTTGTGTGGTAGAGAACCAGTATCGGCGCAT-TGTGCCGGACAGCGGTAACTTACT gamma-Yersinia_int 736 GGGCGTTGTGTGGTAGAGAACCAGTATCGGCGCAT-TGTGCCGGACAGCGGTAACTTACT gamma-Yersinia_fred 736 GGGCGCTGTGCCGTGGAAAACCAGTATCGGCGCAT-CGTGCCCGACAGTGGCAACCTATT gamma-Yersinia_moll 736 GGGCGCTGCGTGGTCGAAAACCAGTATCGGCGCAT-TGTCCCAGACAGTGGCAACTTACT gamma-Serratia_protea 736 CAGCAGAGCCAAACCGAGAACCAATACCGCCGGGT-GGTACCTGAACAGGGCAATCTGCT alpha-Rhodobacter_sphaer 736 GGCCGCTCGGTGATCGAGAACCAGTATGCCCGCGT-GGTGCCCGAGCACGGCAATCCGGT alpha-Rhodobacter_spaer17025 736 GGCCGGGCGGAGGTCGAAAACCAGTATGCCCGCGT-GGTGCCTGAGCATGGCAATCCGGC alpha-Roseovarius-2601 736 GGACGGGCGGAGGTGGAAAACCAGTATGCCCGCGT-GGTGCCAGAGCATGGCAACCCGGT alpha-Paracoccus_deni 736 GGGCGCGCCGAGGTCGAAAACCAATATGCCCGCGT-GGTCCCCGAGGAGGGCAATCCGGT alpha-Xanhobacter_auto 736 GGGACGGCGCGGATCGAGAACCAGTATGCCCGCGT-GGTGCCGGAGCATGGCAATCCGCA actinobacteria-corynebacterium 715 GGCACCTCCGAGGTAGGAAACCAATACGCCCGCGT-TGTGCGCCCCGAGGGCAACACTGC Actinobacteria-RhodococcusRHA1 718 GGCCGCTGCGAGATCGAGAACCAGTACAAGAGGGT-GGTGCGCGCGGAGGGCAACGTGCA Chloroflexi-Acidobacteria 715 GGTCGCGCTGAGGTTGAGAACCAGTACGCGCGCTCGGTG-CGCGAGTTTGGCAATGTTCC Chloroflexi-Solibacter_usi 715 GGTCGCGCCGAGGTGGAGAATCAATATCCGCGCGCGGTATCGCGAGAG-GGTAACGTTGC delta-proteo_MMS1 808 GGCCGGGCCGAAGTGGAAAACCAGTACCGCCGGGC-GGTGCGCCGCCAGGGTAACCCGGC Cyan-SynechococcusWH5701 718 GGCAAGGCCCGGGTGGAGGACGCCTACCCACGGGCCGGCGCGGGGG-AGGGCAATGCCTC Cyan-Synechocystis6803 715 GGACAATTCACCTGCAACAATCAATATCGGCGATC-GGTCCAACCCCAGGGCAATGCCCA chloroflexi-chloroflexus_agg 715 GGCCGTGCCGAACTAGACAACGCCTACGAACGCGC-CGTTCGCCCGGAAGGCAACGTCGC chloroflexi-chloroflexus_aur 715 GGCAAAGCAGAGCTTGACAATGCCTATGAGCGGGC-CGTGCGCCCAGAAGGCAACGTGGC Chloroflexi-Roseiflexus_cas 715 GGACGCGCCGAACTCGACAACGCCTATGAGCGTGC-CGTGCGACCGGAAGGGAACGTCGC Chloroflexi-RoseiflexusRS-1 715 GGGCGCGCCGAACTCGACAACGCCTACGAGCGCGC-CGTGCGATCCGAGGGAAACGTTGC Actinobacteria-Mycobacterium_g 715 GGCCGGCACGAGCTGGAGAACGCCTATCCGCGCGC-GGTCCAGGACACCGGCAACCCGGC Actinobacteria-Mycobacterium_v 715 GGCCGCCACGAGTTGGAGAACGCCTATCCCCGTGC-GGTGCGCGAGGCGGGCAACCCGGC Actinobacteria-FrankiaCcI3 715 GGCCGACACGAGGTGGAGAACGCCTATCCCCGAGC-GGTTCCGGCAGCCGGCAACCCGGC Actinobacteria-FrankiaACN14a 715 GGCCGCCACGAGCTGGACAACGCCTACCCGCGGGC-CGTGCCGGCGGCGGGCAACCCGGC Actinobacteria-Streptomyces_av 715 GGTGAGCACACCGTCGACAACGCGTACGCCCGTGC-CGTCCGCCCGGAGGGCAACCCGGC Actinobacteria-Mycobacterium_s 715 GGTACACCGGAGTTGCGCAACGCCTATTCACGCGC-CGTCACGGCCGAGGGCAACGTCTT Chlorobi-Pelodictyon_lut 715 AGTCAGCCGGAAGTGGTGAACAGCTACGGCCGGGT-TGTGAGCCGGGAGGGCAACCTGGC

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Chlorobi-Pelodicton_lut 715 GGGCGTGCAGAGGTTGTGAACCGCTACGGACGGGT-GGTGAGTCGTGAAGGGAACCGTGT chlorobi-chlorobium_lim 715 GGGCGGGCCGAGGTCGTCAACCGCTATGGCAGGGT-GGTCTCTTCTGACGGCAATATCCA Chlorobi-Prosthecochloris_aest 715 GGTGAGACGGGTGTTGTCAATGCCTATGAGCGCGT-GGTGAGCCGGGATGGCAATGCCGG chlorobi-chlorobium_tep 715 GGCCGGTCGGGCGTGGTGAACGCTTACGGGCGCGT-GGTGAGCCGCGAGGGCAATCCAGA delta-Syntrophus_acid 724 GGGCGGTTCGAGGTCGCCATCCAGTATGCCCGGGG-GGTAAACAGGGAGGGAAATCCCCA delta-desulfotalea_psy 715 GGCGAGTTCAAGGTAGAAAACTGCTACAACAGGGT-AGTATGCAAAGATGGCAATCCACG chloroflexi-dehalococcoides_et 715 GCAACTCCCCGTCTGGACGTCAGCTATTCACGGGC-AGTCAAACCTGAAGGCAATACTGC chloroflexi-dehalococcoides_CB 715 AAAACTCCCCGTCTGGATGTCAGCTATTCACGGGC-AGTCAAACCCGAAGGCAACCTTGC clostridia-desulfitobacterium_ 724 GGCCGTGCGGAGATAGAAATACAATATGATAAGAT-CGTGAAACCGGAAGGCAATCGGGT Clostr-Moorella_therm 736 GGCGAGGCCCGGGTTGCCAACAAGTACACGCGGGT-AGTGCGGGAAGAAGGTAACCTGGA Bacteroides-Robiginitalea_bif 715 GGTAAGGCCGAACTTCAAAATCAATATAGCCGTGT-GGTTAAGCCGAAAGGAAACCCAAA epsilon-Helicobactr_acin 733 AAAGAAGCCAAGCTAGAAATCCAGTATAAAAGAGT-GGTTAGTTATGAGGGGAATGTAAA epsilon-Helicobacter_pylor 733 AAAGAAGCCAAGCTAGAAATCCAATACAAAAGAGC-GGTGAGTTTTGAGGGGAATGTGAA gamma-PhotobacteriumSKA34 739 GGCGAACCGGAACTCGATGTGCAATATACGCGTGC-AGTAACAGAAGAGGGGAATATGAT gamma-Vibrio_ang 739 GGTGAACCTGAACTTGACGTGCAATATACGCGTGC-AGTAACAGAAGAAGGTAATACGGT Gamma-Photobacterium_prof 736 CGCCAAGTGGCCTTACAGGTGCAATACAGTCGTGC-AGCGACCTATGCGGGTAATGAAAC epsilon-Wolinella_succ 733 CAAGAGGCTAGGCTAGAGATTCAATACGCGCGAAG-CGTCACGATGGAGGGGAATCTTAA epsilon-campylobacer_con 715 GGCACTTATGAAGTCTATAACGAGTACGCAAGGGC-GGTCAAAGAAGAGGGCAACCTCAA Aquifex 760 GGGAGGGCTGAGGTTAAAAACAATTACACAAGGTT-CGTTACTTACAAGGGGAACTTGAA gamma-Actinobacillus 739 AATCGTTGTGAAGTTGAAAATCAATACAAGCGGAT-TGTTCAATCCAACGGTAATCTAAT gamma-Manheimia-succ 739 GGGCGTTGCGAAGTTGAAAATCAATATAAGCGAAT-CGTGCACGAACAGGGCAATATGTT gamma-Acidobacillus_succinogen 739 GGGCGTTGCGAAATCGAAAATCAATATAAACGAAT-CGTGCATGAGAACGGCAATGGCTT Cyano-Avar 724 AATCGCTGTGCAGTAGAAAATCAATATAATAGATT-AGTCCAAAAACAAGGGAATCAAAT Cyan-Nostoc7120 724 AATCGCTGTGAAGTAGAAAATCAATATAATAGATT-AGTTCAAAAAGGTGGGAATCAAAT Cyan-Nodularia_spu 724 AACCGTTGTGAAGTAGAAAACCAATATAACAGATT-AGTAGAACCAGCCGGAAACTTAAT Cyan-Lyngbya8106 736 CGACGTTGTGAGGTCGAAAATCAATATAAACGCCT-TGTTCAACCGGACGGAAATCCGAT Cano-trichodesmium_ery 736 GGTCGTTGTGAAGTTGAAAATCAATATAGTCGCAT-AGTAAAAGAAGAGGGTAATAGGGT clostridia-caldicelluosiruptor 669 ATTGAGTGAAGATTTTACAGT------AAAGAATG-AATA---TAAAAGGGTGGTCAAAA clostridia-clostridium_beij 697 ACTGAAAAAAGATTTGAAAATTTATATAAAACTTG-TGTAAGCCAAGAAGGAAACAAAAG Methanobacteriales-Methanospha 670 GATAATCCTAGAATACAAAATGAATATAAACGAGC-TGTAAGAGAAGAAGGTAATATAAA Thermococcales-Pyrococcus_abys 670 GATAATCCTAGAATACAAAATGAATATAAACGAGC-TGTAAGAGAAGAAGGTAATATAAA Methanococcales-Methanocaldoco 706 GGAGAGGCAAAGGTTGAAAATGAATATATTAGAGC-AGTTAAGCCAGAAGGTAATGTTTT Methanococcales-Methanococcus_ 709 GGTGAAGCAAAAGTTGAAAATGAGTACACGAGAGG-AGTGAGGGAAGAAGGAAACGTTAT delta-Lawsonia_intra 718 GGAAAGTACAAAGTAGAAAATGCCTATCCACGGGC-TGTTTATCAAGATGGTAATCCACA epsilon-campylobacter_jej 715 GGCACTTTTAAAGTGTATAATCAATACAAAAGAGC-CGTAAGCAAAGAAGGCAATGTTAA epsilon-campylobacter_ups 715 CAAAAAGCCGAAATTTATAATCAATACTCAAGGGT-AGTTAGTCGCTTGGGTAATGTCAA clostridia-clostridium_therm 685 AATGAATACAGTATGAAAAACATGTATCAAAGTGC-TGTAAGCCCTGAGGGAAATCAAAA clostridia-carboxidothermus_hy 706 AAAGAACCAAGTTATTATAACGCTTATCCCCATGT-CGTAAGGGAACAAGGAAATGTGGA Clostr-Halothermothrix_oren 718 AATGATATCAGGGTAAAAAATCTGTATCCCAGGCT-GGTAAGGCTTGAGGGAAACAAAAA Methanosarcinales-Methanosarci 700 GGGGTTGCAAGGGTGGACAACGGCTATCCGAGGGC-CGTAAAGCCCGAAGGAAACCCGAT Methanosarcinales-Methanosarci 700 GGAATCTCAAGGGTAGATAACGGCTACCCGAGAGC-TGTAAAACCTGAAGGGAACCAGAT Clostridia-Thermoanaerobacter_ 691 AAAAATCCTACAATTGAGATCCAGTACAAAAGGGT-TGTGAAAGAAGAAGGAAATATTGA clostridia-clostridium_cell 718 GGGAGCATTGTGTTTGAGAACATGTATTCTCGAAT-TGTAGCAAGGGAGGGAAACATCAA cyano-crocosphaera_wat 715 GGAAAATATGACTGTGAAAATCAATATATTCGCTC-CGTAAAATCTCAGGGTAACTTTTT Bacteroides_Flavobacteriu_john 712 GGAAAACATGAAGTAGAAAATCAATATACAAGATT-GGTAAAAGAAGAAGGAAACCTGAG

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Proteo-others-MagentococcusMC1 765 TGCGCTCACCTGT-GTTAATAATGTGTTTGATGTG---CAGAGTGTGCATTGGC-GGGGT beta-Nitrospira_multi 759 CGCACAGGCGCAA-CTCGCGGAAGCATTGAATGAC---ACGGATGCCAACTGGC-GCGGC beta-Thiobacillus_denitri 756 GGCGCGTCGAATG-ATGGCCCGCGCGTTCGACGTG---GTCGACGCGAACTGGC-GCGGC alpha-Acidiphilum 765 CGCCCGCCGCCTG-CTCGACGAGGTGATGGAGGTG---ACCGACGCCGCCTGGC-GCGGC Thermococcales-Pyrococcus_fur 786 GGCCCAAGAACTG-ATTTGGAAGTACTTTGAAGTT---AAAGATGCAAAGTGGA-GGGCC Thermococcales-Pyrococcus_hori 786 AGCCCAAGAATTA-ATTCGGAAGTTCTTTGAAGTT---AAAGATGCAAAGTGGA-GGGCC Thermococcales-Thermococcus_ko 798 CGCCCAGAAGATG-ATAGACAAGTTCTTCGAGGTC---GTCGATGCCAAGTGGC-GTGCC Archaeoglobales-Archaeoglobus 780 GGCTTTGGAGGCC-ATGGACAGGGTTTTCAGGAAG---GAAGACTGGAGCTGGA-GGGGG Methanopyrales-Methanopyrus_ka 735 CGCGAAGGAACTG-ATGGAAAGGGCGTTCGAGCCC---GAAGATAGGCCGTGGC-GGGGT Methanomicrobiales-Methanospir 711 GGCCATGGCGGTG-ATGAATATGGTCTTTGAACCA---TGTGATGTTGAGTGGC-GGGGA Sulfolobales-Metallosphaera_se 783 GGCACAACAGGTT-ATGGAAAGAGTTTTCGATCTA---GAGGACG-GATATGTTAGAGGA Thermoproteales-Pyrobaculum_is 834 GGCGCAGTCTTCA-ATACATACAGTGTTTGAAACT---GTAGATACGGCGTGGC-GAGGC Methanobacteriales-Methanother 732 GGCCCAGAAGGTG-ATGGATGAGGTCTTCAAGGTA---ACCGAGAGGGAGTGGA-GGGGA Methanomicrobiales-Methanocull 714 GGCG-AAACGCCTGATGTACGAGGTCTTCGAGCCG---TATGACGTCGAGTGGC-GCGGA Thermoproteales-Thermofilum_pe 858 GGCGCAGGCGGCT-ATACACGAGGCGTTTGAAGCT---GTGGACGACGCGTGGA-GGGGG delta-Anaeromyxobacter 786 GGCGCAGCGCTCG-CTGTGGCGCGCGTTCCGCACC---GTGACCGGCCGCTGGC-GCGGC delta-desulfovibrio_des 777 GGCGCGCAGCATC-ATGTACGAGGTATTCCGTCCT---GCAGACGCGCTGTGGC-GCGGC delta-geobacter_metalli 783 GGCCCAGGAGGTG-ATCCGGGAGGTCTTCACCCCC---TGCGATGCCCCCTGGC-GCGGC delta-Pelobacter_propion 783 GGCCCAGTCTGTG-CTGGCCCAGGTGTTCAGCGTC---TGTGATGCCGAGTGGC-GCGGG delta-geobacterFRC32 783 GGCCAGGGAAGTC-ATGTCACAGGTATTTACTCCC---TGTGATTGCCATTGGC-GCGGC proteo-others-Mariprofundus_fe 783 GGCACAGGCGCTG-ATGCAAGAAGTGTTTGAGCAGCGTCCGATGTTTGAATGGC-GCGGA alpha-Stappia_agg 801 GGCTCAGAAGCTG-GTTGACGAGGTGCTGGAGCTGCGCCGGACCTTTGAATGGC-GCGGG beta-Methylibium_petro 801 TGCGCAGGCGCTG-GTGTCCGACATCTTCGAGCTGCGGCCGAGTTTCGAGTGGC-GTGGC beta-Ralstonia_metall 801 GGCCCAGGCCTTG-GTGTCGGAAGTCTTCGAGCTGCGCTGCTCCTTCGAATGGC-GCGGC beta-Rhodoferax_ferri 801 GGCGCAGGACCGG-GTCGCCGAGGTGTTCGAGTTGCGCCGCGAATTCGAGTGGC-GCGGG beta-dechloromonas_aro 801 GGCGCAACAACTG-GTCGCCGAAGTCTTCGAGATGCGCAAGCATTTCGAATGGC-GCGGC beta-Azoarcus 804 GGCGCAGGCCCTG-GTCGCCGAGGTCTTCGAGCTGCGCGACAGCTACGAATGGC-GCGGC beta-Burkholderia-viet 798 CGCGCAGGCGCTG-ATGGACGACGTGTTCGAGGCTCGCGAGAGCTTCGAGTGGC-GCGGC alpha-Magnetospirillum_mag 801 GGCCAAGTCCCTG-GTGGCCGACATGTTCGAGCTTCGGCGCGCCTTCGAGTGGC-GGGGC alpha-Bradyrhizobium_jap 810 CGCCAAGGAGGAG-GTCTCGCAGGTCTTCGAGCTGCGTGAGCAGTTTGAATGGC-GCGGG alpha-BradyrhizobiumBTAi1 786 CGCAATCAAGGAG-GTCGCCGCGCTGTTCGAGCTGCGCGACCAGTTCGAGTGGC-GCGGT alpha-Rhodopseudomonas_palBisA 801 CGCCCAGGGCGAA-GTCGCACACTACTTTGAGCTGCGCGAGGATTTTGAGTGGC-GCGGC alpha-Rhodopseudomonas_palCGA0 801 CGCGCAGGCTGAA-GTCGCGGCGATCTTCGAGCTGCGCGACAGTTTCGAATGGC-GCGGC beta-Burkholderia_phy 783 GGCGCAGGCGCTC-GTCGCGCAGACATTCGAGTTGCGAAACGATTTCGAATGGC-GCGGA alpha-Rhodospirillum_rub11170 813 CGCCCAGGCCCTG-CTTAACGAGGTTTTCGAGGTCAAGGCCAGCGCGGAATGGC-GCGGG beta-Burkholderia_xeno 804 GGCGCTGTCGGCG-ATCGGGCAGGTGTTCGAGCCGCGCGAGTTTTTTGAATGGC-GCGGG alpha-Sphingopyxis_alask 795 TTCGCTGGCTGCG-GTTACGGAAGTATTCGAACTGCGGGAATTCTTCGAATGGC-GCGGG gamma-Shigella_flex 795 GGCGCAACAGGCG-ATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGC-GCGGC gamma-Shigella_sonn 795 GGCGCAACAGGCA-ATTGCTGATGTGTTCTGTGTCAACGGCGACAGCGAATGGC-GCGGC gamma-Shigela_dys 795 GGCGCAACAGGCA-ATTGCTGATGTGTTCTGTGTCAACGGCGACAGCGAATGGC-GCGGC gamma-EcoliK12 795 GGCGCAACAGGCG-ATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGC-GCGGC gamma-Ecoli_CFT073 795 GGCGCAACAGGCG-ATTGCCGATGTGTTCTGTGTCAACGGCGACAGTGAATGGC-GCGGC gamma-salmonella_ent 795 GGCGCAGCAGGCC-ATCGCCGATGTGTTCTGCGTTAATGGCGACAGCGAGTGGC-GCGGC gamma-salmonella_LT2 792 GGCGCAGCAGGCC-ATTGCCGATGTGTTCTGCGTTAATGGCGATAGCGAGTGGC-GCGGT gamma-enterobacter_638 795 GGCGCAAAACGCC-ATCGCCGAGGTTTTTGCCGTAGAAGGTGACAGCGAATGGC-GCGGT gamma-erwinia_caro 795 AGCGCAAAAAGCG-ATAGCGGATGTTTTCATGCTGAAAGGTGACGCCGAATGGC-GTGGT gamma-Yersinia_ber 795 GGCACAACAGGCA-CTGACAGAGGTTTTTGTTGCTAAAGCCAGCAGCGAATGGC-GTGGC gamma-Yersinia_int 795 GGCACAACAGGCA-CTGACAGAGGTTTTTGTTGCTAAAGCCAGCAGCGAATGGC-GTGGC gamma-Yersinia_fred 795 AGCACAGCAAGCA-CTGGATGAAGTGTTTATAGTTAAAGCTAGCAGCGAGTGGC-GTGGG gamma-Yersinia_moll 795 GGCGCAACAGGCG-CTGGCCGAGGTGTTTACCGCCAAAGCCAGCAGCGAATGGC-GCGGC gamma-Serratia_protea 795 GGCACAGCAGGCG-ATGAGCGAAGTCTTTGTGCTGGGCGGCGGCAGCGAATGGC-GCGGC alpha-Rhodobacter_sphaer 795 CAGCCTCGCCGCC-ATCGCCGACGTCTACGAGCGGCGGCCGAGCTTCGAATGGC-GGGGC alpha-Rhodobacter_spaer17025 795 GAGCCTTGCCGCG-ATCGCGGATGTCTATGAGCGGCGGCCGAGCTTCGAGTGGC-GGGGT alpha-Roseovarius-2601 795 CTCGCTCGCCGCC-ATTGCCGATGTCTACGAGGAGCGCCCGTCCTTCGAGTGGC-GGGGC alpha-Paracoccus_deni 795 TAGTCTGGCCGCC-ATTGCCGATGTCTATGAACGGCGCCCCAGCTTTGAATGGC-GCGGT alpha-Xanhobacter_auto 795 GGCGCTGAAGGCG-GTGGAAGAGGTCTACGAGCCGCGCGAGACCTTCGAGTGGC-GCGGG actinobacteria-corynebacterium 774 CGCACTCAACTTG-ATGTCACGAGTGTTCCGTGTTCGCGAGACCTTCGAATGGC-GCGGT Actinobacteria-RhodococcusRHA1 777 GGCGCTGAAGTTG-ATGGCCGAGACGTTCGAACTGCGTCCGCACTTCGAATGGC-GTGGG Chloroflexi-Acidobacteria 774 GGCGCAGGATCTC-ATCGGCCAGGTCTTTCGTGTG---ATTCCGCGCAAGTGGC-GCGGC Chloroflexi-Solibacter_usi 774 GGCGCGGAGGATG-ATCGCAGAGGTGTTTGACGTG---GCGGACCGCAGCTGGC-GAGGC delta-proteo_MMS1 867 GGCGCAGGCGGCG-GTGGAGGAGGTGTTTATGGTG---GTTGATCAAGAGTGGC-GCGGA Cyan-SynechococcusWH5701 777 CGCCCGCGCCCTG-ATCTCACAGGTGTTCACGACC---TGCGACCAGCACTGGC-GCGGC Cyan-Synechocystis6803 774 TGCTCAGAAAATT-ATTGACCAAGTGTTTGAGCCA---GTCGATCGCCATTGGC-GGGGT chloroflexi-chloroflexus_agg 774 CGCCAAACAAATG-CTTGCCGATGTCTTCACCGTC---ACCGACCGCACTTGGC-GTGGA chloroflexi-chloroflexus_aur 774 CGCCAAACAGATG-CTGGCCGACGTTTTCACCGTC---ACCGACCGGGCATGGC-GCGGG Chloroflexi-Roseiflexus_cas 774 GGCGCAACAGGTG-CTGTCTGAGGTCTTTGAGGTG---ACCGACCGGGCATGGC-GCGGC Chloroflexi-RoseiflexusRS-1 774 GGCACAGCAGGTG-TTGTCTGAGGTGTTCGAGGTG---ACCGACCGGGCATGGC-GCGGC Actinobacteria-Mycobacterium_g 774 GGCGAAGGCGATG-CTGCTCGACGTGTTCGACGTG---ACCGACCGGACCTGGC-GCGGC Actinobacteria-Mycobacterium_v 774 CGCCAAGGCGATG-CTGCTCGACGTGTTCGAGCTC---ACCGACCGGGCCTGGC-GCGGG Actinobacteria-FrankiaCcI3 774 CGCCATCGCGATG-CTGCGGGATGTTTTCGAGGTG---GCCGACCGGGCCTGGC-GGGGC Actinobacteria-FrankiaACN14a 774 CGCCATCGCGATG-CTGCGGGACGTCTTCACGGTG---ACCGACCGGGCCTGGC-GCGGC Actinobacteria-Streptomyces_av 774 CGCGCAGGCCATG-CTCGAGGATGTCTTCGAGGTC---ACCGACCGGGCCTGGC-GAGGC Actinobacteria-Mycobacterium_s 774 CGCGCAGCAGACG-CTGTCCGAGGTGTTCACGGTG---ACCGACCGGCAGTGGC-GCGGC Chlorobi-Pelodictyon_lut 774 TGCGCGTGGGACA-ATCGACAAGGTTTTTGAGGTT---GTTGATCGTCAGTGGC-GAGGC

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Chlorobi-Pelodicton_lut 774 GGCGCAAGGGATT-ATCAGCCAGGTCTTTGAAGTA---ACCGACCGGCAGTGGC-GGGGG chlorobi-chlorobium_lim 774 TGCAAGAGAGCTT-GTCTCGACCGTTTTCGAGGTT---GCCGACCGTCAGTGGC-GGGGT Chlorobi-Prosthecochloris_aest 774 GGCGTTACGGGTG-ATGGACGACGTGTTTGAAATT---GCCGACCGGACGTGGC-GGGGG chlorobi-chlorobium_tep 774 GGCGCGGCGGGTG-ATGCAGGAGGTGTTCGAGGTC---GCCGACCGTCCGTGGC-GCGGC delta-Syntrophus_acid 783 GGCCCTGAACATG-ATGGGCGAGGTCTTCGAAACA---AGGGAGGCAGAGTGGA-GGGGA delta-desulfotalea_psy 774 TGCACGAGCCATG-GTCGAACGCATCTTTGAGCCC---TGCGATATGCAGTGGC-GCGGC chloroflexi-dehalococcoides_et 774 CGCCCTTAAGATT-ATGGATGAAGTATTTGATATC---TGCCCGGCCAACTGGC-GGGGG chloroflexi-dehalococcoides_CB 774 CGCCCTGAAGATT-ATGGACGAAGTATTTGATATC---TGCCCGGCCAACTGGC-GCGGC clostridia-desulfitobacterium_ 783 AGCTCAGGCTCTC-ATCCATAAGATCTTTGAACCG---GTTGATGCAGCATTTC-GGGGA Clostr-Moorella_therm 795 TGCTCGCAAGCGC-ATTGCGGAGTGTTTCGAGGAG---GTCGATGTCCCCTGGC-GCGGC Bacteroides-Robiginitalea_bif 774 AGCCCAGGAACTC-ATCCGTAAGATTTTCATGGTC---TCTGACCGGGAATGGC-GGGGT epsilon-Helicobactr_acin 792 AGCGCAAGAGTTA-GTGCGTGAATGCATGGAAATAAGAGAAAATTTTGAATGGA-GAGGC epsilon-Helicobacter_pylor 792 AGCGCAAGAGTTA-GTCAATGCTTGCATGGAAGTTAGGGAAAATTTTGAATGGA-GAGGT gamma-PhotobacteriumSKA34 798 TGCTCAACAAGCG-GTTAAACGTTGTTTTGATGTGCGTGATAGTTTCCGCTGGC-GTGGC gamma-Vibrio_ang 798 TGCTCAGCAAGCG-GTTAAACGTTGTTTTGATGTGCGTGACAGTTTCCGCTGGC-GTGGT Gamma-Photobacterium_prof 795 GGCCCAGCGTCAG-GTTGACCGTGTTTTCGATGTTCGCGATCAATTCAACTGGC-GAGGG epsilon-Wolinella_succ 792 GGCTCAGGCGATG-ATAGAGCGCTACTTTGAAAAGCGCGAGAGCTTCACATGGC-GAGGA epsilon-campylobacer_con 774 GGCAAAAGAGCTC-ATCGCTAAGTACTTTGAGCCGTGCGA---CTTTGTCTGGA-GAGGT Aquifex 819 GGCTCAAAACCTC-GTTGCGGAATTATTTGAACTGAGGAAGGAATTTGAGTGGA-GAGGA gamma-Actinobacillus 798 CGCACAGCAGGCA-ATGCAAGAAGTCTTTCAATTGAAAAAAAGTAGCGAATGGC-GAGGA gamma-Manheimia-succ 798 GGCGCAAAAAGCT-ATGGCGGAAGTGTTTCAACTTAAAGCCCGCAGCGAATGGC-GAGGT gamma-Acidobacillus_succinogen 798 GGCACAACGCGCC-ATGGCGGAAGTTTTCCGATTGAAAGCCCGCAGCGAATGGC-GCGGC Cyano-Avar 783 AGCACTAGAAGCT-ATGCACAAAGTGTTTGCAGTCCGGGAAACATTTGCATGGC-GTGGC Cyan-Nostoc7120 783 AGCACTAGCAGCC-ATGCACAAAGTATTTGCAGTTCGGGAAAAATTTGCATGGC-GTGGC Cyan-Nodularia_spu 783 AGCATTGCAAGCC-ATGAACCAAGTATTTGCAGTGCGAAAAAACTTTGATTGGC-GAGGC Cyan-Lyngbya8106 795 GGCGTTAGAGGCG-ATTTCTAAAGTGTTTGAAGTGCGAGAAGAATTTGAATGGC-GAGGA Cano-trichodesmium_ery 795 AGCACTTGAAGCC-ATGAAACGAGTATTTGAAGTGCGAGATACGTTTGAGTGGC-GTGGT clostridia-caldicelluosiruptor 719 AGGAAGGCAATGT-GATTGCAAAAGGTT---ATATTGAAAAGTA--TTTTGAGA-GAACG clostridia-clostridium_beij 756 AGCAAATGAATTA-ATAAGTGAAGTATTCACATATTGCGAA-CA--TGAATGGA-GAGGA Methanobacteriales-Methanospha 729 AGCTCAAAAAGCT-ATGGATGAAGTATT--TAGAGTAACAACTGC-TGAATGGA-GAGGT Thermococcales-Pyrococcus_abys 729 AGCTCAAAAAGCT-ATGGATGAAGTATT--TAGAGTAACAACTGC-TGAATGGA-GAGGT Methanococcales-Methanocaldoco 765 AGCTCAAAAAATA-ATAAATGAAGTTTT--TGAAAGCATAGATGT-TCCTTGGA-GAGGT Methanococcales-Methanococcus_ 768 TGCTCAAAAAATT-ATGAATAAAGTTTT--TGAACCCGCTGATGT-TGCATGGA-GAGGC delta-Lawsonia_intra 777 AGCACGTAATATT-TTATATAAGGTGTT--TCAAGTAGGAGACTC-TCAATGGA-GAGGA epsilon-campylobacter_jej 774 AGCACAAAATTTA-GTAAAAAAATACTT--TAGGGTGTGTGATTT-TGAATTTC-GTGGC epsilon-campylobacter_ups 774 GGCGCAAAATTTG-GTTCAAAAATATTT--TAAGCCTTGCGATTT-TGAATTTA-GAGGT clostridia-clostridium_therm 744 GGCGAAATCTTTG-ATTGACAAATATTT--TGAAGCTGCAGACGA-CTATTGGC-GGGGA clostridia-carboxidothermus_hy 765 AGCCCAGAGGATT-ATCGAGGAAGTTTT--TGAAAAAGGCGATGC-CAATTGGC-GGGGA Clostr-Halothermothrix_oren 777 AGCACAGGAGCTT-ATTAAAGAAATTTT--TACACCAGTGGATAG-TTACTGGC-GTGGT Methanosarcinales-Methanosarci 759 AGCCCTGAAAATG-ATGAATGAGGTATT--CGAAACCTCGGAGTC-GGAGTGGA-GAGGC Methanosarcinales-Methanosarci 759 TGCCCTTAAAATA-ATGAATGAAGTATT--TGAAACCTCAGATTC-TGAATGGC-GAGGC Clostridia-Thermoanaerobacter_ 750 AGCGCAGAAGATA-ATTGAAGAAGTTTT--TGAACCCTGTGACAG-CACATGGA-GAGGG clostridia-clostridium_cell 777 AGCACAGCAAAAA-ATGTACGAGGTTTT--TGAACCTGTTGATGT-AGTTTGGC-GTGGA cyano-crocosphaera_wat 774 AGCACAACAACTC-ATTCAAGAAATCTT--TGAAGTGGTTCCCCG-TCGTTGGC-GAGGT Bacteroides_Flavobacteriu_john 771 AGCAAAAGAAGTC-GTAAATACCATTTT--TACTATTGGAACTCA-GGAATGGC-GAGGC

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Proteo-others-MagentococcusMC1 820 ATTGGTCGGGTGCCAGACTCGGGCTACGCCCTACGTGAA-GCTTGGCACCATCGAGATGC beta-Nitrospira_multi 814 ATTGGTGTTATCCCATCTTCCGGTTTCAGTCTCCAAAAGCGCTTCGCAA-AGAACGATGC beta-Thiobacillus_denitri 811 ATCGGCGTGATCGCTCGCTCGGGATACGCACTCAAACCGGGCCTCGCGC-AGCACGACGC alpha-Acidiphilum 820 ATCGGCGTCATCCCCCGCTCCGGCTTCGCCCTGCGCGAAGGC-TGGCGCGTGCACGACGC Thermococcales-Pyrococcus_fur 841 CTAGGAGTAATTCCAAGGAGCGGATTGGAACTTAAGAAAGAGTGGAAGG-AGCTAGAAAT Thermococcales-Pyrococcus_hori 841 TTGGGAATAATCCCGAAAAGTGGTTTAGAGCTTAGAAAGGAATGGAAAG-AGCTAGAGAT Thermococcales-Thermococcus_ko 853 CTCGGCGTTTTCCCGAAGAGCGGCCTCGAGCTGAGGAAGGAGTGGAAGG-ATTTCGAGAT Archaeoglobales-Archaeoglobus 835 CTTGGAGTGGTGAAGGGGAGTGGTGGAGCATTGGCAAAAAGATTCGAGC-AGTTCGATGC Methanopyrales-Methanopyrus_ka 790 TTCCCGACCATCCCCGAGTCGGCGTTGAAGCTGCGTGAGGACCTCGCAG-ATCACGATGC Methanomicrobiales-Methanospir 766 TTCCCGGTTATTCCTCAGTCAGGACTCCGGCTTCGTGAAGAGTTTTCTG-CGTGGGATGC Sulfolobales-Metallosphaera_se 838 GTTGCGGTTTTCCCTAAGGCGGGATTCAGATTAAAGGAGGAGTTCAGTA-AAAAGGATGC Thermoproteales-Pyrobaculum_is 889 ATAGGCTATATCCCAAAGAGCGGCCTCGCGCTTAGGGAGGAGTTCAAGC-AGTACGACGC Methanobacteriales-Methanother 787 TTCCCGGTCATACCCGAATCAGTCTATGAGATAAGGGATGAGTTCTCTG-AATTCAATGC Methanomicrobiales-Methanocull 769 TTCCCGGTGATCCCGGCCTCCGGCCTCCGCCTGAAACCGGAGTTCGAGG-GCTACGACGC Thermoproteales-Thermofilum_pe 913 ATAGGGTTCCTGCCGCTCAGCGGGCTTAGGCTCAGGGAGAAGTACGGGC-GCCTGGACGC delta-Anaeromyxobacter 841 ATCGCCGACGTCCCGGGCGGCAACCTCGAGCTGGTGCCGGAGCTCGCCG-CGCTCGACGC delta-desulfovibrio_des 832 ATCGGCACCATACCCGACAGCGGGCTGGTGTTCACCGACGCCTACAGAC-AATTCGACGC delta-geobacter_metalli 838 ATCGGGGTCATCCCGGGGAGCGGCCTGCGGATCGCCGACGCCTATGCCG-TGTTTGACGC delta-Pelobacter_propion 838 ATCGGCGTCATTCCGGGCAGTGGCCTGGAAATCGCAGCCGACTATGCCG-CCTATGACGC delta-geobacterFRC32 838 ATCGGCATCATCCCCGGCAGTGGGCTGAAGATCAGAAGAAGCTATGCCC-GGTTCGACGC proteo-others-Mariprofundus_fe 841 CTGGGCTCACTGCCTGAGAGCGCCGTGCAAATCAGAAGCGAATACGCAG-CCTTTGATGC alpha-Stappia_agg 859 CTCGGTTATGTAGCGGACAGTGCCCTGAAAATCCGTGAGACCTTTGCCG-CCTTCGATGC beta-Methylibium_petro 859 CTCGGTGAAGTGCCGTACAGCGCGCTCAAGATCCGCCCCGGCTTCGCCG-CCTTCGATGC beta-Ralstonia_metall 859 CTGGGCGAGGTGCCCTACAGCGCCCTGCGCATCCGCGCCAGCTACCGCC-TGTTCGACGC beta-Rhodoferax_ferri 859 CTCGCCGTGGTGCCTTATTCCGCTTTGATGATCCGTGAGAAATACGGCA-GCTTCGATGC beta-dechloromonas_aro 859 CTGAACATCCTGCCTTACTCGGCGCTGCGCATTCGCAGCCAGTTCGCCG-AATTTGATGC beta-Azoarcus 862 CTCGGCAGCCTGCCGCAGTCGGCGCTGAAGATCCGCGCCGAGTTCGCCG-CATGGGATGC beta-Burkholderia-viet 856 CTGGGCGAGGTGCCGGCCAGCGCCCTGCGGATCCGCGCGAAATACGCGC-GCCACGACGC alpha-Magnetospirillum_mag 859 CTCGGACTGGTGCCCTATTCGGCGCTGCGCCTCAAGGAGCCCTATGCCG-CCTGGGACGC alpha-Bradyrhizobium_jap 868 CTTGGACTGGTACCCAACAGCGGACTGAAGCTGAAGCAATCATATGCAA-AATTCGACGC alpha-BradyrhizobiumBTAi1 844 CTCGGCAGCATTCCAGCGAGCGCGTTGAAGCTGCGTTCGGCCTTAGCGC-ATCTCGATGC alpha-Rhodopseudomonas_palBisA 859 TTTGGCCGGGTGCCGGCCAGCGGACTGCGGCTGCGCAAGGAGTTTGCGG-CGTTCGACGC alpha-Rhodopseudomonas_palCGA0 859 TTCGGCCGCGTGCCGGCGAGCGGCCTGCGGTTGCGCGAGGCCTATGCGG-CCTTCGACGC beta-Burkholderia_phy 841 CTCGGGCTCGTGCCCGATAGCGCACTCGCGATCCGCACACCGTTTGCGC-AATGGGACGC alpha-Rhodospirillum_rub11170 871 CTTGGCATGGTCGCCGACAGCGCCCTGGCCTTGCGTCCGGCCTATGCCC-GCTTCGACGC beta-Burkholderia_xeno 862 CTTGGATCGATTGAGCATTCGGGTGTGCGGATTCGCGATGCGTTTGCCG-CGTTCGATGC alpha-Sphingopyxis_alask 853 CTGGGCTCGATTGACCATTCTGGCGTCCAGATTCGCGAGGCCTATGCGG-CCTGGGATGC gamma-Shigella_flex 853 TTAGGCGTGATTGAATCTTCTGGCGTGCACCTGACGCCGGATTATCAAC-GATTCGATGC gamma-Shigella_sonn 853 TTAGGCGTGATTGAATCTTCTGGCGTGCACCTGACGCCGGATTATCAAC-GATTCGATGC gamma-Shigela_dys 853 TTAGGCGTGATTGAATCTTCTGGCGTGCACCTGACGCCGGATTATCAAC-GATTCGATGC gamma-EcoliK12 853 TTAGGCGTGATTGAATCTTCTGGCGTGCACCTGACGCCGGATTATCAAC-GATTCGATGC gamma-Ecoli_CFT073 853 TTAGGCGTGATTGAATCTTCTGGCGTGCACCTGACGCCGGATTATCAAC-GATTCGATGC gamma-salmonella_ent 853 CTGGGCGTGATTGAATCCTCCGGCGTCCACCTGACGCCGGAGTATCAGC-GCTTTGACGC gamma-salmonella_LT2 850 CTGGGCGTGATTGAATCCTCCGGCGTCCACCTGACGCCGGAGTATCAGC-GCTTTGACGC gamma-enterobacter_638 853 CTGGGACTGATTGCTGAATCTGGCGTGCATTTAACCCCCGCGTATCGCG-CGTTCGACGC gamma-erwinia_caro 853 TTGGGGATTATTGGTGAATCCGGCGTGACATTAACCCCAGATTACCAGC-AGTTCGATGC gamma-Yersinia_ber 853 TTGGGTGAAATAGCGGATTCCGGCGTACAACTCAGCCCTGACTATATCG-CTTTCGATGC gamma-Yersinia_int 853 TTGGGTGAAATAGCGGATTCCGGCGTACAACTCAGCCCTGACTATATCG-CTTTCGATGC gamma-Yersinia_fred 853 TTGGGCGAAATTGCCGATTCCGGTGTGCAACTTAGCCCGGCATATCAGG-CTTTTGATGC gamma-Yersinia_moll 853 TTGGGTGAAATAGCCGACTCCGGTGTCCAGCTCAGCCCGGCTTATCAGG-CGTTTGATGC gamma-Serratia_protea 853 CTTGGCACCATTGCTGATTCGGGTATTGCCTTAACCCCGGCCTATAGCA-CCTTTGATGC alpha-Rhodobacter_sphaer 853 CTCGGCGAGATCGACGCCTCGGGCCTGCGTATCCGCGAGACCTATGCAG-CCTTCGATGC alpha-Rhodobacter_spaer17025 853 CTGGGCGAGATCGATGCCTCGGGCCTGCGCATCCGCGAAACCTACGCCG-CCTTCGACGC alpha-Roseovarius-2601 853 TTGGGCGAGATCGACGCCTCCGGCCTGCGCATCCGCGAGGCCTATCGCG-CCTGGGATGC alpha-Paracoccus_deni 853 CTGGGCGAGATCGACGCCAGCGGATTGCGCATCCGCCCGAAATACGCAG-CCTTCGACGC alpha-Xanhobacter_auto 853 CTCGGCTCCATCGCCGGCTCCGGGGTGCGGCTCCGGGCGCCCTATGCTG-CGTTCGATGC actinobacteria-corynebacterium 832 CTTGGCTGGATCCCCAACTCTGGCCTCGGAATCGCAGACGAGTATGCGC-AGTGGGATGC Actinobacteria-RhodococcusRHA1 835 CTCGGGTTCATCTCGCAGAGCGCACTCAAGGTCCACCGGAACTACGCCG-AGTTCGACGC Chloroflexi-Acidobacteria 829 GTTGGCGAGATCCCGCAGAGCGGCTTCGGTCTCGCAGCGGAGTTCGCCG-AATACGACGC Chloroflexi-Solibacter_usi 829 ATTGGGATCATACCGAAGAGCGGCTACAAGCTGGGCTATGCCTTCCGCG-AGTACGATGC delta-proteo_MMS1 922 CTGGGGCCGATCCCCCGCAGCGGCTTGCAGTTGCGGCCGCAATTTGCCG-CCTTTGACGC Cyan-SynechococcusWH5701 832 CTGGGGGTGCTGCCCGACAGCGGGCTGTGTCTGCGCCCTCCCTACGACA-GCCTCGACGC Cyan-Synechocystis6803 829 TTGGGATTAATTCCGGCCAGCGGTTTGGGTTTAAGGCCAGCATTTGCCC-CCTGGGATGC chloroflexi-chloroflexus_agg 829 ATTGGGCGCATCCCGCGCAGCGGTTGGCGGCTCAGTGACCGCTACGCCG-AATTCGATGC chloroflexi-chloroflexus_aur 829 ATCGGGGTTATTCCCCGCAGTGGCTGGAAGTTGAGCGAGCGGTACGCCG-CTTTCGATGC Chloroflexi-Roseiflexus_cas 829 ATCGGCGTTATCCCGCAGAGCGGTTGGCGCCTGCGCAACGCCTACCGCG-CCTACGACGC Chloroflexi-RoseiflexusRS-1 829 ATTGGGGTCATCCCGCAGAGCGGCTGGCGTTTGCGCGACGCATACCGCG-CCTACGACGC Actinobacteria-Mycobacterium_g 829 ATCGGCATGATCGCAAGCAGTGGCTGGCGGTTGTCGCCCGCCTACCGGC-ATTTCGACGC Actinobacteria-Mycobacterium_v 829 ATCGGGGTGATCCCCGGCAGTGGCTGGCGGCTGTCCCACGCCTACCGCG-ACTATGACGC Actinobacteria-FrankiaCcI3 829 ATCGGTGTCATCCCGGGCAGCGGCTGGCGCCTGTCGCCGGCCTACCGCG-ACTTCGACGC Actinobacteria-FrankiaACN14a 829 ATCGGGGTGATCCCGGACAGCGGCTGGCGGCTGTCGGCGGCCTACGCGC-AGTTCGACGC Actinobacteria-Streptomyces_av 829 ATCGGTGTGATCCCCGACAGCGGCTGGCGGCTGTCACCCAAGTACCGCG-ACCACGACGC Actinobacteria-Mycobacterium_s 829 ATCGGCATGATCCCGCGTTCGGGGTGGACGTTGTCACCGCAGTACGCGG-AGTTCGACGC Chlorobi-Pelodictyon_lut 829 GTCGGCCTTATACCGGAAAGCGGTCTGGGGTTGAGGTCGGAGTATGCCG-GATTCGATGC

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Chlorobi-Pelodicton_lut 829 ATTGGCCTTATCCCCGCAAGCGGACTTGGCCTCAGGGAATCATACCGCC-GCTATGATGC chlorobi-chlorobium_lim 829 ATCGGACTTATTCCGGGGAGCGGCCTCTCTCTGCGCCGGGAGTTTTTAC-GCTTCGATGC Chlorobi-Prosthecochloris_aest 829 ATTGGCGTTATTGCCCGGAGCGGTTTGAGGATCCGCGAAAAATATGCTG-CTTTTGATGC chlorobi-chlorobium_tep 829 ATCGGCGTCATTCCGGCAAGCGGGCTTGTGCTGCGCCCGGAGTACAAGC-GTTTCGACGC delta-Syntrophus_acid 838 CTGGGAAGAATCCCCCAGAGCGGTCTTTTCATCCGGGAGAGGTATGCTG-ACTTCGACGC delta-desulfotalea_psy 829 CTTGGCATGATTCCGGAAAGTGGTTTGAAAATCCGGGAAGAATATGGTG-AGTTTGATGC chloroflexi-dehalococcoides_et 829 ATAGGTATTTTACCCCAAAGCGGCCTCTGCCTGCGGCCTGAATTTGCCG-CTTTTGATGC chloroflexi-dehalococcoides_CB 829 ATAGGTATTTTGCCCGAAAGCGGTCTCTGCCTGAAACCAGAATTTGCCG-CTTTTGATGC clostridia-desulfitobacterium_ 838 CTCGGTATCATTCCTCTGGGAGGGTTGGGGGTCCGTAAGGAATATGAGG-ATTGGGATGC Clostr-Moorella_therm 850 CTTGGAGTTATTCCTGAAAGCGGACTTTCACTACGGGAAACTTTTCGGG-ATTTTGATGC Bacteroides-Robiginitalea_bif 829 ATAGGCACTATTCCTGCCAGTGGGTTTGAGGTAAGACCCAAATATGCCG-CCTTTGATGC epsilon-Helicobactr_acin 850 TTGGGGAATATCAAAAATTCCGCTCTAAAACTCAAAGAAACATTCGCCT-CTTATGACGC epsilon-Helicobacter_pylor 850 TTGGGGAATATCAAACGATCCGCTCTCAAACTCAAAGAAGCGTTCGCTT-CTTATGACGC gamma-PhotobacteriumSKA34 856 TTAGGGCCTATTGCTAACTCAGCTTTAAAACTCAATGCTGCTTATCAAC-ACCGTGATGC gamma-Vibrio_ang 856 TTAGGGCCTATTGCTAATTCTGCGTTGAAATTAAATGCAGCCTACCAAC-ACCGTGATGC Gamma-Photobacterium_prof 853 CTGGGTAATATTCCTCGATCGGCTTTACAGCTTAAACCGTCCTTCCAGC-ATCGCGATGC epsilon-Wolinella_succ 850 ATCGGTCCTATTCCCTGCTCGGCTTTGCGCCTTAGAGAGGAGTTTAAGG-AGTGGGACGC epsilon-campylobacer_con 829 CTTGGCGAGATAGCGCAAAGCGGTATGAAGTTAAAAGACGAGTTTGCCT-ATCTTGACGC Aquifex 877 CTCGGCACAATACCTTACAGCGCCCTGAAGATAAGGAAAGAGTACGAAG-AGTTTGACGC gamma-Actinobacillus 856 TTAGGCGAAATTGCGGAATCAGGGGTTGAGCTTACCGAGCAGTATGCGC-AATTCGATGC gamma-Manheimia-succ 856 CTTGGTGAAATTGAAGAATCCGGCGTGGAATTAACCGTCGATTATCGCC-GCTTTGATGC gamma-Acidobacillus_succinogen 856 TTGGGCGAAATTGAAGAATCCGGCGTGGAACTGACACCGGCATATCAGC-AATTTGATGC Cyano-Avar 841 TTAGACGAAATTCCGGATTCTGGGTTAAAAATTCGCCCAGAATATGCCC-AATTTGATGC Cyan-Nostoc7120 841 TTAGATGAAATCCCGGATTCTGGGTTAAAAATTAGAGAAGAATATGCCC-AATTTGATGC Cyan-Nodularia_spu 841 TTAGGTGAAATACCAGAATCAGGCTTAAAAATCCGGACTGAATACACCC-AATTCGATGC Cyan-Lyngbya8106 853 CTCGGAGATATTGCCCAATCTGGACTTAAAATTAATTCAAATTATGCCC-AATTTGATGC Cano-trichodesmium_ery 853 TTGGGAGAAATTCCCGAGTCCGGATTAAAAATTACTGATGCTTATTCCT-GTTTTGATGC clostridia-caldicelluosiruptor 772 TCAGCATTTTTTAGGGGTTTGGGATTGATACCTGGCGGAGGTTTGAAGA-TAAAAGATGA clostridia-clostridium_beij 811 ATAGGAGTTATTAAAGATTCTGGATTTACTTTGAGGGAGAAATATAAAA-AATATGATGC Methanobacteriales-Methanospha 784 TTCCCTGAAATACCAAATTCCATATATGATCTTAAAAAGGAATTTGATG-ATCATAATGC Thermococcales-Pyrococcus_abys 784 TTCCCTGAAATACCAAATTCCATATATGATCTTAAAAAGGAATTTGATG-ATCATAATGC Methanococcales-Methanocaldoco 820 TTCCCAGTTGTTAAAAATGGTGGTTTTGGATTGAGGGAGAAGTATAAGA-AATTTGACAT Methanococcales-Methanococcus_ 823 TTTCCAGTCATTAAAGACGGCGGTATGAAATTAAGAGAAGAATTCAAAA-AATACGATGT delta-Lawsonia_intra 832 TTAGAGTTTCTCCCAATGAGTGGCCTTACTTTTAGAGATCATTATAAAA-AATTTGATGC epsilon-campylobacter_jej 829 TTAGGACTTATTAAAAATGGAGGCTTGGAGTTAAAAGAAGAATTTAGCG-CTTACGATGC epsilon-campylobacter_ups 829 TTAGGACTTATAAAAAATGGCGGCTTAGAGCTTAGAGAAGAATTTGCAA-GATATGATGC clostridia-clostridium_therm 799 ATCGGAATAATAAAAAACTCGGGGCTAAGGCTAAGAACGGAATACAGGG-ATTTTGATGC clostridia-carboxidothermus_hy 820 ATAGGTAATATTCCGTTGTCAGGATTATTTATTCGTGAAAGGTATTCCC-AATATGATGC Clostr-Halothermothrix_oren 832 CTGGGTACCATTACAGGCTCTGGATTGAAACTCAGGAAAGTTTATCATA-GATATTCTGC Methanosarcinales-Methanosarci 814 ATTGGAAACATAGAGAATTCAGGGCTCGTGCTCAGGCAAGAGTACGAAG-AAAAAGACGC Methanosarcinales-Methanosarci 814 ATAGGGAAAATAGAAAATTCGGGGCTTGTGCTCAGGAAAGAGTTTGAAG-AAAAGGACGC Clostridia-Thermoanaerobacter_ 805 CTTGGCGAGATAAAAGGTTCAGGCCTTAAGATAAGGGACAAATACAAAG-ACTTTGATGC clostridia-clostridium_cell 832 ATAGGCATGATTCCCAGATCCGGTTTAGGGCTTGTAAAAGATTATGAAC-AGTTCGATGC cyano-crocosphaera_wat 829 ATTGGAGAAATACGGGAGAGTGGCTTAGGTTTACGCCCAGAATACTCCC-TCTATGATGC Bacteroides_Flavobacteriu_john 826 ATTGGTAAAATTGAAAACAGCGGACTTGTATTGAGAGAAAATTATAAAA-TGTATGATGC

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Proteo-others-MagentococcusMC1 879 CCGCAAACGTTTTCACGCCCT-----------GGCAACCTTTCAGC-----CTGAG---A beta-Nitrospira_multi 873 GCGACTTCAATTCCCCGATTT-----------CGATACAGAGAACC-----GCAAGCGCG beta-Thiobacillus_denitri 870 ACGCGTCCTGTTCGACGACCA-----------CACCGATCCGGCGC-----GCTGGCGCG alpha-Acidiphilum 879 CCGCGCCCG---CCACGTCAT-----------CGCCGCGCCGCCCC-----GCCGCCGCG Thermococcales-Pyrococcus_fur 900 TAGAACTTATTACAATCCCGA-----------GGTTCCAAAG---C-----TCCCAGATC Thermococcales-Pyrococcus_hori 900 TAGAAACTATTATGATCCTGA-----------TGTCCCAAAG---C-----TTCCAGATC Thermococcales-Thermococcus_ko 912 AAGGAGCTTCTACAAAGTAGA-----------GGTTCCCAAGAACC-----TGCCCGACC Archaeoglobales-Archaeoglobus 894 GAGGAAGGTTTTTGAAGATGC-----------CTTCGCCGATTTTC-----AGCCTGTGG Methanopyrales-Methanopyrus_ka 849 GATTGAG-ATTGGAATACGGC-----------CGGATTACAC----------CCTGGGAC Methanomicrobiales-Methanospir 825 CCAGAAGAAATTTGATGTAAC-----------GAT--TAAA-------------CCGACG Sulfolobales-Metallosphaera_se 897 CAGAGTTCAATACGGCTTAAA-----------GGACAGGACG-----------AAATCTA Thermoproteales-Pyrobaculum_is 948 CTTTGAATACTTCGGAATCCC-----------AGACCTAACGCCGG-----AGAGATGGC Methanobacteriales-Methanother 846 CCGGGAGAAATTCGATATAGA-----------GGTTGAAGAT------------ACGGTT Methanomicrobiales-Methanocull 828 GCAGAAGAAGTACGATATCGA-----------GA--------TCCG-----GCACGTGG- Thermoproteales-Thermofilum_pe 972 CTTGAGGCACTTCGGGATCGA-----------GGAGCTGACGCCCG-----AGAAGTGGC delta-Anaeromyxobacter 900 CCGCAAGCGCTTCCGCATCGAC--------ACGGCCGGCGTGCGCG-----ACGCGGCCG delta-desulfovibrio_des 891 CATGGAACAGCAGGAACTGAC-------------CC------------------TGCGGG delta-geobacter_metalli 897 CGAGAAGGCGATTCCCGTGGC-------------------------------GGTGGAGG delta-Pelobacter_propion 897 GGAAAAAGTCCTGCCGGTTTC-------------------------------CGTGGAGG delta-geobacterFRC32 897 TGAGAAGGTGCTTCCAGTCAA-------------------------------GGTAGAGG proteo-others-Mariprofundus_fe 900 CGA------GAAAC-------------------GATTTAACATGAA-----AGAGATTGC alpha-Stappia_agg 918 CGA------AGAGC-------------------GTTTTGCGCTCAG-----CCCCAAGGA beta-Methylibium_petro 918 CGA------GCGGC-------------------GCTTCCAGCTCGG-----CTACCAGCC beta-Ralstonia_metall 918 CGA------GGCCC-------------------GCTTCGGCCTCGC-----CTACCGGCC beta-Rhodoferax_ferri 918 CGA------GCGCC-------------------GCTTCAAGATGGA-----TTACCAATC beta-dechloromonas_aro 918 CGA------AAAGC-------------------GTTTTCCGCTCGG-----TTATGCATC beta-Azoarcus 921 CGA------GGCCA-------------------GGTTCGGCATCGG-----CTACACCCC beta-Burkholderia-viet 915 CGA------AGCGC-------------------GTTACGGGCTGGT-----CTACCGGCC alpha-Magnetospirillum_mag 918 CGA------GCGCC-------------------GCTGGGACGTGCC-----CCAGGACAG alpha-Bradyrhizobium_jap 927 CGA------GACGC-------------------GCTTTGCGATCCA-----TGACCTGCG alpha-BradyrhizobiumBTAi1 903 CGA------GTTGC-------------------GTTTCGCCATGGC-----AGAACGAGT alpha-Rhodopseudomonas_palBisA 918 CGA------ATTGC-------------------GCTTCGGCCTCGC-----CGAGATTTC alpha-Rhodopseudomonas_palCGA0 918 CGA------GCGCC-------------------GCTTCGCGATGGC-----GGAGATCTC beta-Burkholderia_phy 900 GGA------AAAGC-------------------GCTTCAATCTGTC-----CGCTCAGCC alpha-Rhodospirillum_rub11170 930 CGA------GAAAC-------------------GCTTTCCGTGCGA-----TCTGCCGCC beta-Burkholderia_xeno 921 GGA------GCGCAA------------------GTTTCCTGTAGC------CAACCTGCA alpha-Sphingopyxis_alask 912 TGA------GCGAAA------------------ATTTGCCGTTGCA-----TCGCCTAGC gamma-Shigella_flex 912 CGA------AGCAC-------------------ATTTCCGCCCGGC-----ACCGCAGCA gamma-Shigella_sonn 912 CGA------AGCAC-------------------ATTTCCGCCCAGC-----ACCGCAGCA gamma-Shigela_dys 912 CGA------AGCAC-------------------ATTTCCGCCCGGC-----ACCGCAGCA gamma-EcoliK12 912 CGA------AGCAC-------------------ATTTCCGCCCGGC-----ACCGCAGCA gamma-Ecoli_CFT073 912 CGA------AACAC-------------------ATTTCCGCCCGGC-----ACCGCAGCA gamma-salmonella_ent 912 CGA------AGCGC-------------------ATTTCCGCCCAGC-----GCCACAGCA gamma-salmonella_LT2 909 CGA------AGCGC-------------------ATTTCCGCCCAGC-----GCCGCAGCA gamma-enterobacter_638 912 CGA------AGCGC-------------------ATTTTCGCCCGCA-----GCCACAGCA gamma-erwinia_caro 912 GGA------ACGTT-------------------ATTTTCGCCTACA-----GCCGCAAGA gamma-Yersinia_ber 912 CGA------GTTAC-------------------GTTTCAAACCGCA-----ACAGCAGCA gamma-Yersinia_int 912 CGA------GTTAC-------------------GTTTCAAACCGCA-----ACAGCAGCA gamma-Yersinia_fred 912 TGA------ACTAC-------------------GTTTCAAGCCACA-----GCAGCAAAG gamma-Yersinia_moll 912 CGA------GTTGC-------------------GCTTCAACCCGCA-----ACAGCAGCG gamma-Serratia_protea 912 GGA------ACGAC-------------------GTTTCCGTCCGCA-----ACACCGGCA alpha-Rhodobacter_sphaer 912 CGA------ACGGAAGTTCG-------GGATCGGCTACGGCACTCC-----GGCCGCGCC alpha-Rhodobacter_spaer17025 912 CGA------GCGCAGGTTCG-------GCGTCGGCTATGGCGCAGG-----CCGGGCGCC alpha-Roseovarius-2601 912 GGA------GGAGAAATTCG-------GCATCGGCTACGCGGTGGG-----GCGGAAAAA alpha-Paracoccus_deni 912 CGA------GGAAAAATTCG-------GCATCGGCTATGCGGTGGC-----GCGCAATCC alpha-Xanhobacter_auto 912 GGA------GAAGC-------------------GCTTCGCCGTGCC-----GGACGTGAA actinobacteria-corynebacterium 891 AGA------AAAGC-------------------TTTTCGACGTCCC-----CGGCCGCCG Actinobacteria-RhodococcusRHA1 894 CGA------GGAGA-------------------AGTTCTCGATGCC-----GGGGGTGCG Chloroflexi-Acidobacteria 888 GGA------GTTGCGCTT-----CGGT------GTCGCGGACCT----------CACGGT Chloroflexi-Solibacter_usi 888 GGA------CCGAATTTT-----CGAA------GT-GCAACACA----------TTCGCA delta-proteo_MMS1 981 CGCCGCCAAACTGGGCCCCGAGCCGGCCCCGGCAGCCCAAACGGCCCCGG-CAGCGCCGG Cyan-SynechococcusWH5701 891 GGC------ACGGCGCTTCCTGCCGGC------GGCCCGGCCAG---------GCACCGA Cyan-Synechocystis6803 888 CGC-----AGTTAAATTC---GCCAATT----TATTGCAAACCAT-------GGCCCCAA chloroflexi-chloroflexus_agg 888 CGA------ATTCCGATT--------------CAACGTCCACGAC--------ATCCAAA chloroflexi-chloroflexus_aur 888 TGA------ACAGCGCTT--------------CGATGTCAGCGAC--------ATCCAGA Chloroflexi-Roseiflexus_cas 888 CGA------GGCGCGCTT--------------CGCGGTTGGCGAC--------ATTCAGA Chloroflexi-RoseiflexusRS-1 888 TGA------GACGCGCTT--------------CGCCGTGAGCGAC--------ATTCAGA Actinobacteria-Mycobacterium_g 888 GGA------GCACCGCTT--------------CGCCGCCACCGAC--------CTCCACA Actinobacteria-Mycobacterium_v 888 CGA------ACAGCGGTT--------------CGCCGTCAGCGAC--------ATCCATA Actinobacteria-FrankiaCcI3 888 GGA------GCTGCGGTT--------------TGCCGTCGCGGAT--------GTCCACA Actinobacteria-FrankiaACN14a 888 GGA------GACCCGCTT--------------CGCCGTCCGCGAC--------ATCCACA Actinobacteria-Streptomyces_av 888 CGA------GTACCGCTT--------------CTCGGTCGACGGG--------ATCCAGA Actinobacteria-Mycobacterium_s 888 CGA------ACTGAGGTT--------------CGGTGTCGGTCAT--------CTGCAGG Chlorobi-Pelodictyon_lut 888 TGA------GAAAAGGTT--------------TCACCTCTCCCAT--------ATGAGCA

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Chlorobi-Pelodicton_lut 888 TGA------AAAAAAGTT--------------TGATGTTGCACAC--------CTCTGTG chlorobi-chlorobium_lim 888 GGC------CCGGAAGTT--------------CGCCGTGGCTCAT--------ATCTGCA Chlorobi-Prosthecochloris_aest 888 TGA------ACGCAAGTT--------------TGATGTCGGTCAT--------ATCGAAC chlorobi-chlorobium_tep 888 CGA------AAAGCGCTT--------------CGATGTGGGCCAC--------ATCGCCC delta-Syntrophus_acid 897 ACTAAGAAAAGGAAA---------------------CCTGCCTGAG-------ATCCGTT delta-desulfotalea_psy 888 AGA------GAGAC-------------------GTTTAGAGATAGA-------GCTAACA chloroflexi-dehalococcoides_et 888 CGA------AAAAAACTT-------------TGAGATAAAACTTTCC----GAGCCCAG- chloroflexi-dehalococcoides_CB 888 TGA------GAAAAACTT-------------TGAAATAAAACTCTCC----GAACCCAG- clostridia-desulfitobacterium_ 897 ----------TGCGAA---------------AAAATTCGCGCTCCCG----GAGTTTGAG Clostr-Moorella_therm 909 CCG------ATTTAAACT-------------AAATATAGAACCGGCC----CCGCCAACA Bacteroides-Robiginitalea_bif 888 CAA------TCTAAAGT-----------------TTACTATCCAGG--------TCGAAA epsilon-Helicobactr_acin 909 TGAAAAAGCGTTTAAAGA---------------ATATTTAACCCAC-------AAAACCA epsilon-Helicobacter_pylor 909 TGAAGAAGTCTTTAAAGA---------------ATATTTAAGCCAC-------AAAACCT gamma-PhotobacteriumSKA34 915 TGAAATTATTTTTGC-AG---------------ACTGCTTACCTAT-------GACACCG gamma-Vibrio_ang 915 TGAAATACGTTTTGC-GG---------------ATTGCTTACCCAT-------GACGCCG Gamma-Photobacterium_prof 912 TGAAGTGAAGTTCGCTGG---------------ACATTTCACCCAT-------ATTGACA epsilon-Wolinella_succ 909 GGAGCGCTATTTTGCCTC---------------GATTCTTCATCAT-------GAAGAGA epsilon-campylobacer_con 888 TAGAGTGCAGTTTGA----------------------CTGCAGCGT-------AGAGAGC Aquifex 936 GGAAAAGGTCTTTGAGG-------------------TTGAACTTCC-------AGAGCCA gamma-Actinobacillus 915 CGAGAAATATT-------------------TTAATAC---------------------GC gamma-Manheimia-succ 915 GGAGATCTACT-------------------TTAACAG---------------------TA gamma-Acidobacillus_succinogen 915 GGAAATCTATT-------------------TTAACAG---------------------CC Cyano-Avar 900 TGAATTAAAAT-------------------TTACCAC---------------------TC Cyan-Nostoc7120 900 AGAATTAAAAT-------------------TTACTAT---------------------TC Cyan-Nodularia_spu 900 AGAAAATAAAT-------------------TTATTAT---------------------TC Cyan-Lyngbya8106 912 AGAAATAAAAT-------------------TTGATGT---------------------TT Cano-trichodesmium_ery 912 TGAGGCTAAGT-------------------TTTGTCT---------------------GC clostridia-caldicelluosiruptor 831 GTTTAGCGAATATTC-------------AATTGATATATC-----------------ATA clostridia-clostridium_beij 870 TTCTGAAAGATTTGG-------------AATAAATTTAA------------------GTA Methanobacteriales-Methanospha 843 TAGAGTAAAATATGA-------------TATGGAACT---------------------TC Thermococcales-Pyrococcus_abys 843 TAGAGTAAAATATGA-------------TATGGAACT---------------------TC Methanococcales-Methanocaldoco 879 CTATGAGCA-------------------TGAGGATAT---------------------TC Methanococcales-Methanococcus_ 882 TCATGAAAA-------------------TATTGATGT---------------------TC delta-Lawsonia_intra 891 GTTAGAACGAT-------------------TTGATAT---------------------AA epsilon-campylobacter_jej 888 GAGTAAAAAAT-------------------TTGATTG---------------------TA epsilon-campylobacter_ups 888 GAGTAAGCTGT-------------------ATGATTG---------------------CG clostridia-clostridium_therm 858 CGGGAGCAC-----------------------AATTTT--------------------TG clostridia-carboxidothermus_hy 879 GCGAAAAAAAT-------------------ATGGTTT---------------------GA Clostr-Halothermothrix_oren 891 CGAAAAAAAGT-------------------TTCAGTT---------------------TG Methanosarcinales-Methanosarci 873 CTCAAAGAAGTATGAAGA----------TTTTTATTCGGCTTCCCTTGCCGAGATAAAGG Methanosarcinales-Methanosarci 873 TGCAAAAAAGCATGAGGA----------TCTGTATGCTTCTGCCCTTGAAGATGTCCGAA Clostridia-Thermoanaerobacter_ 864 AGCCTTAAAAT-------------------TTGACATAA------------------AGC clostridia-clostridium_cell 891 TGCAAAAAAAT-------------------TTGATAT---------------------AA cyano-crocosphaera_wat 888 CTTAAAACGCTTTGA----------------AAGCCCCTTATC---------------CT Bacteroides_Flavobacteriu_john 885 ----GAACATC--------------------AAATTTTCTATT---------------GA

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Proteo-others-MagentococcusMC1 920 T---------GGGCGATATGCCCAACGACTGTCTTTGTGCCTCTATTGTTATGGGTAAGC beta-Nitrospira_multi 917 C---------TGGCCAGATGCCGCCCGGTTGCGAATGCGCGAGCGTAGTCCTTGGAAGAA beta-Thiobacillus_denitri 914 C---------CGGCGAAATGCCGCCCGGATGCGACTGCGCGCAAGTCGTTCTCGGCAAGA alpha-Acidiphilum 920 C---------CGGCGAGATGCCGCCGGGCTGCGACTGCGCCAGCGTCGTCCTCGGCCGCC Thermococcales-Pyrococcus_fur 941 T---------TGAAAAAGGA---------TGTCTCTGTGGGGCAGTCCTTAGAGGATTAG Thermococcales-Pyrococcus_hori 941 T---------TGAAAAGGGT---------TGCCTGTGTGGTGCGGTTCTTAGAGGATTAG Thermococcales-Thermococcus_ko 956 T---------CGAAAAGGGC---------TGCCGCTGCGGCGCCGTGCTGAGGGGACTGG Archaeoglobales-Archaeoglobus 938 A---------AGACAAAAGCAAGAGGTTATGCAGGTGCGGGGACGTGCTGAAGGGCATCG Methanopyrales-Methanopyrus_ka 887 A---------CGACGAATCC---------TGCATATGTGATCGGATACTGCGAGGGTTGG Methanomicrobiales-Methanospir 859 A---------AGAAACAAGCCGGA-----TGTATCTGTGATAAAATCCTCCGGGGTATTG Sulfolobales-Metallosphaera_se 935 A---------GGACGAATATGTAGCTGGCGCGAGATGTGGAGAGGTCGTTATGGGACTTA Thermoproteales-Pyrobaculum_is 992 G---------CTACGACTTGCCGCCTGGTTGTAAATGTGCAGAAGTCAACCTTGGCAAAG Methanobacteriales-Methanother 883 G---------ATACACCGGCGGGA-----TGCATATGCGGTGCTGTATTGAGGGGTGTTG Methanomicrobiales-Methanocull 863 ---------------ACAAGCATTCCGCCTGCATCTGCGACAAGGTGCTGCGCGGCATCG Thermoproteales-Thermofilum_pe 1016 G---------GAAGGACACTCCTCCCGGCTGTAGGTGCGCCGAGGTGACGCTCGGGAAGG delta-Anaeromyxobacter 947 C---------GGAGGAGGAGGCGAAGGGGTGCATCTGCGGGCGGATCATGCTCGGCCTCG delta-desulfovibrio_des 920 A---------CGTGCCCAACACACCCGGATGCAGATGCGGCGACGTGCTCAAGGGCAAAA delta-geobacter_metalli 926 A---------GACCCGGGAACACGCGGGATGTCTCTGCGGCGAGATCCTCAAGGGGAAAG delta-Pelobacter_propion 926 A---------GACCCGGGAGCACCAAGGGTGCCGCTGCGGCGATATCCTCAGGGGGAAGG delta-geobacterFRC32 926 A---------GCAGGAGGAGCAAAAGGGTTGCAGGTGCGGCGATGTCTTGAAAGGGAAAA proteo-others-Mariprofundus_fe 930 ----------CACCGCCGATATCAAAGGGTGCATCTGCCCGGCCGTCCTGCGCGGCGTCA alpha-Stappia_agg 948 ----------ATCGCGCGAGGTCAAATCCTGCGAGTGTCCGGCTATCCTGCGCGGGGTGA beta-Methylibium_petro 948 ----------GGTGGCCGACAACAAGGCCTGCGAATGCGGCGCCATCCTGCGCGGTGTGA beta-Ralstonia_metall 948 ----------GGTGCCCGACAACAAGGCCTGCGAATGCGGCGCCATCCTGCGCGGCGTGA beta-Rhodoferax_ferri 948 ----------GGTGCCCGACAACAAGGCCTGTGAATGCGGGGCCATTCTGCGCGGCGTCA beta-dechloromonas_aro 948 ----------GGTGCCCGATCACAAGGCCTGCGAATGCGGGGCCATCCTGCGCGGCGTCA beta-Azoarcus 951 ----------GGTCGCCGACCACAAGGCCTGCGAGTGCGGCACCATCCTGCGCGGCGAAA beta-Burkholderia-viet 945 ----------CGTCGCCGACCACCGCAAATGCGAGTGCGGCGCGATCCTGCGCGGCCTCA alpha-Magnetospirillum_mag 948 ----------CCCGCCCGACAACAAGGCCTGCGAATGCGGCGCCATCCTCAGGGGCGTCA alpha-Bradyrhizobium_jap 957 ----------TGTCGCAGACAATCCGGCCTGCGAATGCTGCGCGATCCTGCGTGGCGCCA alpha-BradyrhizobiumBTAi1 933 ----------CGTCGCCGACAACCCGGCCTGCGAATGCCCGGCGATCCTGCGCGGACTCA alpha-Rhodopseudomonas_palBisA 948 ----------GGCGGCCGACAATCCGGCCTGCGAATGCGGCACCATCCTGCGCGGCATCA alpha-Rhodopseudomonas_palCGA0 948 ----------CGCCGCCGACAATCCGGCTTGCGACTGCGGCGCGATCCTGCGCGGCATCA beta-Burkholderia_phy 930 ----------GGTCGCTGACGTGAAGGCCTGCCAGTGCGGCCAGATTCTGCGCGGCCACG alpha-Rhodospirillum_rub11170 960 ----------CCCCCAGGTCAATCGCGCCTGCCGCTGCCCCGATATCCTGCGCGGCCTGA beta-Burkholderia_xeno 951 ----------GTTGGCCGATCCGCAGGCGTGTCAGTGCGGCGAGGTCCTTAAAGGCGTCA alpha-Sphingopyxis_alask 943 ----------ATTC-CGGATCCCAAGGCTTGCCAGTGCGGCGAAGTGCTGAAAGGCGCGA gamma-Shigella_flex 942 ----------GGTCTGCGATGACCCGCGCGCGCGTTGTGGTGAGGTATTAACGGGCAAAT gamma-Shigella_sonn 942 ----------GGTCTGCGATGACCCGCGCGCGCGTTGTGGTGAGGTATTAACGGGCAAAT gamma-Shigela_dys 942 ----------GGTCTGCGATGACCCGCGCGCGCGTTGTGGCGAAGTCTTGACGGGCAAAT gamma-EcoliK12 942 ----------GGTCTGCGATGACCCGCGCGCGCGTTGTGGTGAGGTATTAACGGGCAAAT gamma-Ecoli_CFT073 942 ----------GGTCTGCGATGACCCGCGTGCGCGTTGTGGCGAAGTCTTGACGGGCAAAT gamma-salmonella_ent 942 ----------GGTCTATGACGATCCGCGCGCCCGCTGCGGCGAAGTGCTGACCGGCAGAT gamma-salmonella_LT2 939 ----------GGTCTATGACGATCCGCGCGCCCGCTGCGGCGAAGTGCTGACCGGCAGAT gamma-enterobacter_638 942 ----------GGTTTGTGATGACCCGCGAGCCCGCTGCGGAGCGGTCCTGACCGGTAAAT gamma-erwinia_caro 942 ----------AACCAGTGACGATCCGCGGGCATGCTGTGGAGACGTATTAACCGGGCGCT gamma-Yersinia_ber 942 ----------GGTGGCAGATTCTCCACGATCTCGCTGTGGGGATGTGCTGACTGGGCGTT gamma-Yersinia_int 942 ----------GGTGGCAGATTCTCCACGATCTCGCTGTGGGGATGTGCTGACTGGGCGTT gamma-Yersinia_fred 942 ----------AGTGGCAGATGATCCACGATCCCGCTGTGGTGATGTGCTGACTGGGCGCT gamma-Yersinia_moll 942 ----------AGTGGCCGATGATCCACGATCCCGTTGCGGCGATGTGCTGACCGGGCGCT gamma-Serratia_protea 942 ----------GGTGGCTGACGATCCTCGTGCCCGCTGTGGAGAGGTGCTGACCGGGCGTT alpha-Rhodobacter_sphaer 954 ----------CCGGCAGGAGCCCGAGGGATGTGCCTGCGGCGCGGTGATGACCGGCCGGA alpha-Rhodobacter_spaer17025 954 ----------CCAGCCAGAGCCCGAAGGCTGCGCCTGCGGCGCGGTGATGACGGGGCGGA alpha-Roseovarius-2601 954 ----------GGTCGAGGAGCCCGAGGGCTGCGCCTGCGGCGCGGTGATGACGGGCCGGA alpha-Paracoccus_deni 954 ----------CGCACCCGAACCCGAGGGCTGTGCCTGTGGCGCCGTCATGACGGGGCGGG alpha-Xanhobacter_auto 942 ----------GGTGGCGGACCCCTCCTCCTGCCGCTGCGGCGAGGTGCTCACCGGCGCGG actinobacteria-corynebacterium 921 ----------AATCCCCGACCCCGTCGCCTGCGAGTGCGGCTCCGTTTTGACCGGAAAAA Actinobacteria-RhodococcusRHA1 924 ----------GGTGGCTGATCCGAAGGCGTGTCAGTGCGGTGAGGTCCTCAAGGGCGTCA Chloroflexi-Acidobacteria 921 ----------AGAAGAGGACCGCGAG---TGCATCGCCGGAGAGGTTTTGCGTGGTGTGA Chloroflexi-Solibacter_usi 920 ----------CGCGTGAGCCGGAGATC--TGCATTAGCGGGCAGGTGCTGAGGGGGCTGA delta-proteo_MMS1 1040 AGCAGGCCGCCGATGCCGGCGCCAACCCCTGTATCAGCGGCGCCATTTTGCGGGGTATCA Cyan-SynechococcusWH5701 930 -------CACCGACACCGACGCCCAAGGCTGCATCAGCGGCCGGGTGCTGCAGGGGTTAG Cyan-Synechocystis6803 929 ----------CGATGGGAGAAACAGTG--TGTATTAGCGGGGAAATTTTACAGGGACAAC chloroflexi-chloroflexus_agg 920 ----------CGAGCGAGTCGCCGCTA--TGTCGGAGTGGTGAAGTGCTGCAAGGATTGC chloroflexi-chloroflexus_aur 920 ----------CCACCGAGTCGGCACTA--TGCCGGAGTGGTGAGGTATTGCAGGGACGCC Chloroflexi-Roseiflexus_cas 920 ----------CGCGCGAGTCGCCAATC--TGCCGCAGCGGTGAGGTGCTTCAGGGTATGC Chloroflexi-RoseiflexusRS-1 920 ----------CGCGCGAGTCGCCGATC--TGTCGCAGCGGTGAGGTGCTCCAGGGCATGC Actinobacteria-Mycobacterium_g 920 ----------ACGAGGAATCGGCGATC--TGCCGCTCCGGCGAGGTTCTGCAGGGACTGA Actinobacteria-Mycobacterium_v 920 ----------CCGAGGAGTCCGCGGTG--TGCCGCTCGGGCGAGGTGCTGCAGGGCTTGA Actinobacteria-FrankiaCcI3 920 ----------CGGCCGAGTCGTCGGCG--TGCCGATCGGGCGAGGTGCTCCAAGGCCTGA Actinobacteria-FrankiaACN14a 920 ----------CCGAGGAGTCCTCGGTG--TGCCGCTCCGGCGAGGTGCTGCAGGGCCTGA Actinobacteria-Streptomyces_av 920 ----------CGCGCGAACCGGCCGAA--TGCCGCAGCGGCGAGGTTCTGCAGGGAATGC Actinobacteria-Mycobacterium_s 920 ----------TCGCCGAATCGGCCGAG--TGCCGCAGCGGCGAGGTGCTGCAGGGGCTGT Chlorobi-Pelodictyon_lut 920 ----------CCGAGGAGTCGCCCCTC--TGCATGAGCGGCAGCATCCTGCAGGGGGTAA

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Chlorobi-Pelodicton_lut 920 ----------CCGCCGAGTCGCCCCTC--TGCATGAGCGGGAGTGTGCTGCAGGGTGTTC chlorobi-chlorobium_lim 920 ----------CGGCAGAGTCCCCTCTG--TGCAGAAGCGGTCAGGTTTTGCAGGGGAATC Chlorobi-Prosthecochloris_aest 920 ----------CGAAGGAGTCTTCTCTT--TGCATGAGCGGAGATGTTCTCAGGGGTATCC chlorobi-chlorobium_tep 920 ----------CGCAGGAGTCGCCCCTG--TGCAGGAGCGGCGAGGTGCTGCAAGGCCACC delta-Syntrophus_acid 929 ------------CCATCGAATTTGAAGCGTGCCGATGCGGTGAAATCCTGCGGGGCGTGA delta-desulfotalea_psy 916 GA--------AGTAGCGGAGCCCAAGGGTTGTCTCTGTGGAGAAATACTTAAGGGCCAGA chloroflexi-dehalococcoides_et 924 -------------CCGCGAAACACCCGGCTGTCTGTGCGGTGAAATTATCAAAGGCACCC chloroflexi-dehalococcoides_CB 924 -------------CCGCGAAACACCCGGCTGTTTGTGCGGCGAGATTATTAAGGGCACCC clostridia-desulfitobacterium_ 928 A-----------CCGTAGAAATCCCCGGCTGCCGCTGTGGGAACGTTCTAAAGGGGCAGA Clostr-Moorella_therm 946 C-----------CTCTTTACCCGCCAGGTTGTGCCTGTAAAGAAATTTTACGGGGAAAAC Bacteroides-Robiginitalea_bif 917 A---------AGTGGAGGAGCCCAAAGAATGTATTGCAGGATTGGTTCTAAAAGGCATTA epsilon-Helicobactr_acin 947 T-----------AAAA-GAAAACAAAGCATGCAAATGTGCAGAGATCTTAAAAGGCAACG epsilon-Helicobacter_pylor 947 C-----------TAAA-GAAAACAAAGCATGCAAGTGCGGGGAGATTTTAAAAGGCATCG gamma-PhotobacteriumSKA34 952 A-----------TTGATGATCATAAAGCATGTCAATGCGGTGATATTCTTCGCGGCCTAG gamma-Vibrio_ang 952 A-----------TTGATGATCATAAAGCGTGTCAATGTGGTGATATTCTTCGTGGCTTAG Gamma-Photobacterium_prof 950 C-----------ACCA-GATCATAAGCAATGTCGCTGTGGCGATATTCTCAAAGGTCTAG epsilon-Wolinella_succ 947 T-----------TCCT-GATCATAAATCTTGCAAATGCGGCGACATCTTAAGAGGGGTGG epsilon-campylobacer_con 919 G-----------CTGGCGAGAGCAAGGCTTGCATTTGTGGGCAAATTTTAAGAGGGCTAG Aquifex 970 --------------AAGGAACACCCCGCCTGTATATGCGGAAAGGTAATAAGGGGCGTTG gamma-Actinobacillus 935 AGGTACATGCCGTTGCGGATGATCCTCTCGCTCGCTGCGGAGATGTCTTAACAGGAAAAT gamma-Manheimia-succ 935 AGGCGCAGCAAGTCGCCGATGATCCGAATTCCCGTTGCGGCGATGTGCTAACCGGCAAAT gamma-Acidobacillus_succinogen 935 GAGCGCAAAAAGTGGCGGACGATCCGAATTCCCGTTGCGGCGACGTGCTGACCGGCAAAT Cyano-Avar 920 CTAACTTGAAAGTTGCCGACCATAAAGCCTGTCAGTGTGGCGAAATTCTTCAAGGAGTCC Cyan-Nostoc7120 920 CTAACCTAAAAGTAGCCGACCATAAAGCCTGTAAGTGCGGCGAAATTCTTAAAGGAGTCC Cyan-Nodularia_spu 920 CTAACTTAAAAGTAGCAGACCATAAAGCTTGTAAATGTGGAGAAATTCTCAAAGGAGTTT Cyan-Lyngbya8106 932 CTGATGTGAAAGTTCCTGATGCGGCAGCTTGTCAATGTGGAGAAATTCTCAAAGGAGTAT Cano-trichodesmium_ery 932 CTAACAAAAAAGTTGCTGATGCTAAAGCTTGCCAATGTGGGGAAATTCTCAAAGGTGTAC clostridia-caldicelluosiruptor 861 TGACCACACAAAACAAAAAGAT-AGTCTCTGCAGGTGTGCAGATGTCTTAACAGGGAAAA clostridia-clostridium_beij 899 AGACTTCTGAGAAAAACTATATTAGAGCTTGTGCATGCAGTGAAATTTTATTGGGGAAGA Methanobacteriales-Methanospha 869 CAGATTCTGAAAATG-TTCCACAAGGA--TGTATCTGTGGACCAATACTAAGAGGTATGG Thermococcales-Pyrococcus_abys 869 CAGATTCTGAAAATG-TTCCACAAGGA--TGTATCTGTGGACCAATACTAAGAGGTATGG Methanococcales-Methanocaldoco 899 CAGAGATTAAAGAGAAAATTCCTAAAGGTTGTATATGTGATAAGATTTTGAGAGGAGAGA Methanococcales-Methanococcus_ 902 CAGACATTAAAGAAACAATAAACAAAGCCTGCATTTGCAGTGAAATTTTAAGGGGAGAAA delta-Lawsonia_intra 911 CAGGAGTCAATGTACATCCTAACACTGGTTGTATTTGCGGAGAAATTCTTAAAGGTAAAG epsilon-campylobacter_jej 908 CGGTGCAAAGTAAAAATGAAAGCAAAGCCTGTATTTGTGGGCAAATTTTAAGAGGTTTGG epsilon-campylobacter_ups 908 AGGTTAAAAGTAGAGGCGAAAATAAAGCTTGTATTTGCGGTCAAATTTTAAGAGGCTTGG clostridia-clostridium_therm 875 AAGATGCTGACGCTGCACCTTC---GGGCTGTAAATGTGCTGATGTAATACTGGGAAGAA clostridia-carboxidothermus_hy 899 GAGAAAAGCCGCCGATTGTTCCCCGGGGTTGCAAGTGCGGTGAAATTATGTTGGGAAGGA Clostr-Halothermothrix_oren 911 GGGATACTCAATATAGGCCTGGAAAAGGGTGTATCTGTGGAGAAATATTAAAAGGCAAAC Methanosarcinales-Methanosarci 923 CAAAAGCTGAGAAAAAGGATAAGAAGCGCTGCATCTGTGCAGCGATACTCACAGGGAAAG Methanosarcinales-Methanosarci 923 AGAAAACAGGAGGAAAAGACAAAAAGCGTTGTATCTGCGCAGCTATCCTTACAGGAAAAG Clostridia-Thermoanaerobacter_ 887 CTGTTGGAGATGAAATAGAGATAAAGGGATGTAGATGCGGAGATGTTCTAAAAGGGCGCA clostridia-clostridium_cell 911 AATCTAAAGATATACCTGAGCCAAAAGGATGTCTGTGTGGAGAGGTTCTCAAAGGGAAAA cyano-crocosphaera_wat 917 CTTTAACCCCATCCACAGCCCCACAAGTCTGTATTAGCGGTGAAATCATGCAAGGGTACA Bacteroides_Flavobacteriu_john 906 AAC-AACGAATAGCAAAACGGATAATTTGTGTATTGCAGGCCAAATCCTGACGGGAAATA

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Proteo-others-MagentococcusMC1 971 AGAGCCCCCATG-ACTGCTTGCGTTATGCCCATGATTGCCAGCCGGATAATCCCGTGGGT beta-Nitrospira_multi 968 TAAATCCAAACC-AGTGCAAGATTTATGGCCATGCCTGCACACCGAAAACACCTGTGGGC beta-Thiobacillus_denitri 965 TCTATCCCAACC-AGTGCCGCATCTACGGCAAGGCCTGCACGCCGCGCAAGCCGGTGGGA alpha-Acidiphilum 971 GCCAGCCCGACC-AGTGCCGCCTCTACGGCCGGGCCTGCACGCCGCGCCACCCGGTCGGC Thermococcales-Pyrococcus_fur 983 CCTTACCGACCC-AGTGCCAACACTTTGGAAAGACATGTACACCAAGACATCCGGTAGGT Thermococcales-Pyrococcus_hori 983 CCTTACCTACTC-AGTGCCCACACTTCGGGAAGACCTGTACACCAAGGCATCCCATAGGA Thermococcales-Thermococcus_ko 998 CTTTACCTACTG-ACTGCCCCCTCTTCGGAAAGACCTGCACGCCGAGACACCCGGTCGGG Archaeoglobales-Archaeoglobus 989 CCACACCAAAGG-ACTGCCCGCTGTTCATGAAAGCCTGCAACCCGAGAAACCCCGTTGGA Methanopyrales-Methanopyrus_ka 929 CCGAGCCCCGGG-ACTGCCCGTTGTTCGGCACGAAATGTACTCCGACGGATCCCGTAGGC Methanomicrobiales-Methanospir 905 CAACTCCGGTTG-ATTGTAAACTCTTTGATAAGACATGTACGCCACGGACACCGGTCGGT Sulfolobales-Metallosphaera_se 986 TAGATCCTCCAG-AGTGTCCATTGTATGGGAAAACATGTAAGCCTGAGGATCCGAAGGGA Thermoproteales-Pyrobaculum_is 1043 CCAAGCCTACCG-ACTGCCCGCTCTTTATGAAAGCTTGCACGCCGGATCACCCCGTCGGC Methanobacteriales-Methanother 929 CAAGGCCGGAGG-AATGCAAACTCTTCAAGAATGAGTGCACCCCCACCAGTCCCATCGGG Methanomicrobiales-Methanocull 908 CCCGGCCGTCCG-ACTGCAAGCTCTTCGGGAAGGCCTGCACCCCGCGCACCCCCGTCGGC Thermoproteales-Thermofilum_pe 1067 CTAAGCCCACGG-ACTGCCCCATGTTCATGAAGAAGTGTACCCCCGAGAAGCCGTACGGG delta-Anaeromyxobacter 998 CCACCCCGGAGC-ACTGCGCGCTGTTCGGCACCACCTGCGTCCCGGAGTCGCCGGTGGGC delta-desulfovibrio_des 971 TGCAGCCCGACA-ACTGTCCGCTGTTTGCCAAGGCCTGCACACCCGCCCGCCCCGTGGGA delta-geobacter_metalli 977 CGGCCCCAACCG-ACTGTCCCCTCTTTGGCGGCGCCTGCACCCCCGAGTCCCCCGTGGGG delta-Pelobacter_propion 977 TGTCTCCCTTCG-ACTGTCCCCTCTTCGACCGAGCCTGCACCCCGGAATCACCCGTTGGG delta-geobacterFRC32 977 TTCAACCCTTCG-ATTGTCCTCTTTTCGGCATGCAGTGTACCCCGGAAGGTCCGGTAGGG proteo-others-Mariprofundus_fe 980 ACAATCCGGATG-AGTGCAAACTCTTCGGCACCGCCTGCACCCCCGAAGATCCGAAGGGA alpha-Stappia_agg 998 AGAAGCCGACCG-AATGCAAGCTGTTCGGCACGGTCTGCACGCCGGACAATCCGATGGGT beta-Methylibium_petro 998 AGAAGCCGACCG-ACTGCAAGATCTTCGGCACGGTGTGCACGCCGGAGAATCCGGTGGGG beta-Ralstonia_metall 998 AGAAGCCCACCG-ACTGCAAGCTCTTCGCCACCGCGTGCACGCCGGAGAATCCGATGGGC beta-Rhodoferax_ferri 998 AGCGACCGCAAG-ACTGCAAGATTTTCGGCACCGTCTGCACGCCGGAGAACCCGGTGGGC beta-dechloromonas_aro 998 AGCGGCCGCAGG-ACTGCAAGATTTTCGGCACCGTCTGTACGCCGGAAAACCCGGTCGGT beta-Azoarcus 1001 AGCGCCCGGCCG-ACTGCAAGCTGTTCGGCACCGTGTGCACGCCGGAAACGCCGATGGGC beta-Burkholderia-viet 995 AGCAGCCGCGCG-ACTGCTCGCTGTTCGGCACCGTGTGCACGCCCGAGAATCCGATGGGT alpha-Magnetospirillum_mag 998 AGAAGCCCACCG-ATTGCAAGCTGTTCGGCACCGTGTGCACCCCCGAGAACCCCATGGGC alpha-Bradyrhizobium_jap 1007 AACGGCCGATCG-ATTGCAAGTTGTTTGGCAATATCTGCACGCCGGAAACGCCCATAGGA alpha-BradyrhizobiumBTAi1 983 AGAAGCCGGTCG-ACTGCAAGCTGTTCGGCACGGCGTGCACGCCGGAGACGCCGGTCGGC alpha-Rhodopseudomonas_palBisA 998 AGCGGCCGCACG-ATTGCAAATTGTTCGGCACGTTGTGCACGCCGGAAACGCCGATGGGC alpha-Rhodopseudomonas_palCGA0 998 AGCGTCCGACCG-ATTGCAAATTGTTCGGCACGCTATGCACGCCGGAGACGCCGATGGGA beta-Burkholderia_phy 980 GCAAGCCAACTG-ACTGCGAGATGTTTGGCACGGCGTGCACGCCTGCGGCACCACTTGGC alpha-Rhodospirillum_rub11170 1010 TCGCCCCCGAGG-ACTGCGCGCTGTTTGGCCGAAGCTGCACGCCTAGCGATCCGGTCGGT beta-Burkholderia_xeno 1001 TCAAGCCGCCTC-AATGCAAGGTGTTTGGCACTGCGTGCACGCCGGACAGGCCGCTTGGC alpha-Sphingopyxis_alask 992 TCAAGCCATGGC-AGTGCAAGCTGTTCGGTCGGTCGTGCACACCGGAAACACCGATGGGT gamma-Shigella_flex 992 GTAAGCCGCATC-AATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCATTTGGT gamma-Shigella_sonn 992 GTAAGCCGCATC-AATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCATTTGGT gamma-Shigela_dys 992 GTAAGCCGCATC-AATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCATTTGGT gamma-EcoliK12 992 GTAAGCCGCATC-AATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGT gamma-Ecoli_CFT073 992 GTAAGCCGCATC-AATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGT gamma-salmonella_ent 992 GCAAGCCGCATC-AGTGCCCGCTATTTGGTAAAACATGTAATCCAGAGACCGCGTTCGGC gamma-salmonella_LT2 989 GCAAGCCGCATC-AGTGCCCGCTATTTGGTAAAACATGTAATCCAGAGACCGCGTTCGGC gamma-enterobacter_638 992 GCAAACCGCATC-AGTGCCCGTTATTTGGCAACACCTGCAATCCGCAAACCGCATTTGGC gamma-erwinia_caro 992 GTAAACCCCACC-AATGTTCGCTCTTCGGCACAATCTGCACGCCACAAAGTGCCTTCGGT gamma-Yersinia_ber 992 GCAAGCCTGATC-AATGCCCACTGTTTGGTGGTGAATGTACGCCAGACAATGCCTTTGGC gamma-Yersinia_int 992 GCAAGCCTGATC-AATGCCCACTGTTTGGTGGTGAATGTACGCCAGACAATGCCTTTGGC gamma-Yersinia_fred 992 GTAAGCCGAGCC-AATGCCCACTATTCGGTGGCGAGTGCACGCCAGATAATGCATTTGGG gamma-Yersinia_moll 992 GCAAGCCCGATC-AATGCCCGCTGTTTGGTGGCGAATGCACGCCAGAAAATGCATTTGGG gamma-Serratia_protea 992 GCAAACCGCGTG-ACTGCCCGCTGTTTGGTGGTGACTGTACACCGCAAAATGCTTTTGGC alpha-Rhodobacter_sphaer 1004 TCAAGCCCCCCG-CCTGCCCGCAGTTCGGCCGCGGCTGCACGCCCGAGATGCCGCTCGGC alpha-Rhodobacter_spaer17025 1004 TCAAGCCCACCG-CCTGCCCGCAGTTCGGGCGCGGCTGCACGCCCGAGATGCCCCTCGGC alpha-Roseovarius-2601 1004 TCAAGCCGACCG-CCTGCCCGCAATTCGGCCGCGGCTGCAGGCCCGACATGCCTCTGGGT alpha-Paracoccus_deni 1004 TCAAACCTACGG-CCTGCCCGCATTTCGGCAAGGGCTGCACGCCAGAGATGCCGCTGGGG alpha-Xanhobacter_auto 992 TGAAGCCGTGGG-CCTGCCCGGAGTTCGGCACCGGCTGCACCCCCGAGACGCCGCTGGGC actinobacteria-corynebacterium 971 TCAAGCCGTGGC-AATGCCGTGTCTTTGGCACCGCCTGCACCCCGCAGACCCCCATCGGA Actinobacteria-RhodococcusRHA1 974 TCAAACCGTGGG-AATGCAAGGTGTTCGGGACGGCCTGCACACCGGAGACGCCGATCGGC Chloroflexi-Acidobacteria 968 AGAAGCC-GCAGGAGTGTCCGGCGTTCGGCGGGCGCTGCACGCCAGATCATCCACTGGGA Chloroflexi-Solibacter_usi 968 AGAAGCC-GGTGGACTGTCCTGCGTTTGGAGCGCAGTGCTCGCCGCAGACGCCGCTGGGA delta-proteo_MMS1 1100 AGAAACC-GCTGCAGTGCCCGGCCTTTGCCGGCGCCTGCCACCCGGAAAATCCCCTGGGG Cyan-SynechococcusWH5701 983 CCCGCCCCGATGCCTGTCCCCGCTTCGGCCGGGGCTGCACACCCG-AGCGCCCCCTGGGA Cyan-Synechocystis6803 977 GGAAGCCCAGCG-ATTGTCCAGCCTTTGGTACTATCTGCACCCCAGAACAACCCTTGGGG chloroflexi-chloroflexus_agg 968 TCAAACCAAACC-AATGCCCGGCCTTCGGTAAAGAATGCACACCGCGGACGCCACTTGGC chloroflexi-chloroflexus_aur 968 TGAAACCCAACC-AGTGCCCGGCATTTGGCAAGGAGTGTACGCCCCGCACCCCGCTCGGC Chloroflexi-Roseiflexus_cas 968 TCAAGCCCAATC-AGTGCCCGGCGTTCGGCAAGGAATGCACCCCGCGCACGCCCCTTGGC Chloroflexi-RoseiflexusRS-1 968 TCAAGCCCAACC-AGTGCGCTGCATTCGGCAAGGAATGCACACCACGCACACCACTCGGC Actinobacteria-Mycobacterium_g 968 TCAAACCGCACG-AGTGCACGGCCTTCGGGACCAGCTGCACACCCCGGAACCCGCTGGGC Actinobacteria-Mycobacterium_v 968 TCAAACCGCACG-AGTGTGCGGCGTTCGGCACGGTCTGCACACCTCGCAATCCGCTTGGG Actinobacteria-FrankiaCcI3 968 TCAAGCCGCATG-AGTGCGCGGCCTTCGGACGGCAGTGCACTCCGCGGACCCCGCTCGGC Actinobacteria-FrankiaACN14a 968 TCAAGCCGCACG-AGTGCGCCGCCTTCGGACACCGGTGCACGCCGCGGACGCCGCTCGGC Actinobacteria-Streptomyces_av 968 TGAAGCCGCACG-AGTGCGAGGCCTTCGGCACCGTCTGCACACCCCGTACCCCCCTGGGA Actinobacteria-Mycobacterium_s 968 TGAAACCCAACG-AGTGCCCGGCCTTCGGCAAGACCTGCACACCGCGAAACCCGTTGGGT Chlorobi-Pelodictyon_lut 968 TGAAACCTGATG-GATGTCCCGCCTTCGGCGCGGAGTGCACCCCGCAGCACCCCCTTGGT

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Chlorobi-Pelodicton_lut 968 TCAAGCCCGACG-GCTGCCCTGCATTTGCCCGTGAGTGCACCCCGGAACACCCCCTCGGC chlorobi-chlorobium_lim 968 TTAAACCCGATG-CCTGCGAGGCGTTCGGCAGGGAGTGCACCCCTTTACATCCGCTTGGC Chlorobi-Prosthecochloris_aest 968 TGCAGCCGGAAG-CGTGCCCTGCATTCGGCAAGCAATGTACGCCCCTTTCTCCCCTTGGT chlorobi-chlorobium_tep 968 TCAAACCCTCCG-ACTGCCCGGCCTTCGGCAAAGAATGCACCCCGCAAACCCCGTTCGGC delta-Syntrophus_acid 977 TCTCCCCAGAGG-AATGCCGCCTTTTCGGAAAAGTCTGCACTCCGGCAAACCCGGTGGGG delta-desulfotalea_psy 968 CCATCCCCCCAC-GCTGCCCTCTCTTCAACAAAGGTTGTACGCCAATGAACCCGGTGGGT chloroflexi-dehalococcoides_et 971 GGACACCTCTGG-AATGCAAGCTTTTTTCAAAGGTCTGCACCCCGGAAAACCCGGTCGGG chloroflexi-dehalococcoides_CB 971 GGACACCGCTGG-AATGCAAGCTGTTTTCAAAGGTTTGCACCCCGGAAAATCCGGTCGGG clostridia-desulfitobacterium_ 977 TTACTCCACCTG-AGTGTCCATTATTTGCCCGTAAGTGTACCCCCTTGTCTCCTGTGGGA Clostr-Moorella_therm 995 TTTTACCCCCGG-AATGCCGGCTATTTAGTAATGTATGTACACCTGTCCATCCTGTAGGC Bacteroides-Robiginitalea_bif 968 AAAAACCAAAAC-AGTGTTCACAGTTCGGCAAAAAATGCACGCCCCAAAATCCGATGGGT epsilon-Helicobactr_acin 995 CCAAGCCTTTAG-ATTGCCCACTATTTGCAAAAACTTGCACCCCACAAGATCCGATCGGC epsilon-Helicobacter_pylor 995 CTAAGCCCCTAG-ACTGCTCGTTATTCGCTACAACTTGCACCCCACAAAACCCGATCGGC gamma-PhotobacteriumSKA34 1001 CTAAACCTCAAG-ATTGCAAAGTCTTTGGACGTGGTTGTACACCGCAAACACCACTAGGT gamma-Vibrio_ang 1001 CTAAACCTCAAG-ATTGCAAAGTGTTTGGACGTGGTTGTACACCGCAAACACCATTAGGT Gamma-Photobacterium_prof 998 CCACACCACAAG-AATGCAAAGTGTTTGGTAAAGCCTGTAAACCCAACCGCCCGATGGGC epsilon-Wolinella_succ 995 CCAAGCCCTTTG-ATTGCAAGGTTTTTGCCAAGGCGTGCACCCCAGAAAATCCTCTAGGA epsilon-campylobacer_con 968 CAAAGCCGACTG-ATTGCAAGGTCTTTGGCAAGGTCTGCAACCCACAAAACCCGATAGGA Aquifex 1016 CGAGACCTACGG-ACTGCAAGCTCTTCGGAACAGTATGCACGCCTTCAAACCCGCTCGGT gamma-Actinobacillus 995 GTAAACCGAGTG-ATTGTCCTTTATTTGGGCAAAAATGTAACCCGAATAGTGCCTATGGT gamma-Manheimia-succ 995 GCAAACCTGCCG-ACTGCCCGTTATTTGGCAGTGACTGTAACCCTGATAACGCTTACGGC gamma-Acidobacillus_succinogen 995 GCAAACCCGCCG-ACTGCCCGTTATTCGGTACGAGTTGCAACCCCGATAACGCGTACGGC Cyano-Avar 980 TCAAACCTTGGC-AGTGTAAGGTATTTGGTACAGCCTGCACACCAGAATCACCAATAGGT Cyan-Nostoc7120 980 TCAAACCTTGGC-AGTGTAAAGTATTTGGTACAGCTTGCACACCAGAAACACCAATAGGT Cyan-Nodularia_spu 980 TAAAACCTTGGG-AATGTAAAGTATTTGGTACAGCTTGCACACCAGAAACACCCATTGGA Cyan-Lyngbya8106 992 TAAAACCCTGGC-AATGTAAAGTATTCGGAACCGCTTGCACGCCAGAAACACCCATCGGA Cano-trichodesmium_ery 992 TGAAACCTTGGG-AGTGTAAAGTCTTTGGTACTGCTTGTACTCCTGAAAACCCGATCGGC clostridia-caldicelluosiruptor 920 TAAAGCCATTTG-AGTGTCCTCTTTTTGAGAAGGTATGTACACCAATTTCTCCTAAAGGT clostridia-clostridium_beij 959 AATTACCTAATA-AATGCGAGCTTTTTGGAAAAGGTTGTACACCAGAGCATCCAATAGGA Methanobacteriales-Methanospha 926 CAAGACCAGAGG-ATTGTAAATTATTCCGTGGAGAATGTAATCCACTTCATCCAATAGGT Thermococcales-Pyrococcus_abys 926 CAAGACCAGAGG-ATTGTAAATTATTCCGTGGAGAATGTAATCCACTTCATCCAATAGGT Methanococcales-Methanocaldoco 959 AACTGCCAACTG-ACTGCCCATTGTTTGGAACGGTTTGCACTCCATTAAATCCAGTTGGT Methanococcales-Methanococcus_ 962 AACTTCCGCACG-ACTGTAAACTCTTTGGAAAAACTTGTGATCCGATGAATCCTGTTGGA delta-Lawsonia_intra 971 TTACACCTTCTG-CATGTTTATTATTTGGGAAAGAGTGTACTCCAGAAGAACCGATAGGT epsilon-campylobacter_jej 968 CCAAGCCTTATG-ATTGTAAAGTTTTTGGCAAGGCTTGTACGCCAAGAAGTCCTATAGGA epsilon-campylobacter_ups 968 CAAAGCCTTATG-AGTGCAAAGTTTTTGGCAAGGTTTGCACACCGAAAAATCCCATAGGC clostridia-clostridium_therm 932 TAACCCCTGCAC-AATGCCCTCTGTTCGGCAAGGCCTGCACTCCGTTAAATGCCGTCGGA clostridia-carboxidothermus_hy 959 TGCTACCTCCCG-AGTGCCCTCTTTTTGCCAAAGGGTGTACCCCGGAAAAGCCCGTTGGA Clostr-Halothermothrix_oren 971 AGAAACCAACTG-ACTGCAGATTGTTCGGCATCAAATGTACCCCGATGAAACCAGAAGGG Methanosarcinales-Methanosarci 983 CAAAACCCTCCC-AGTGCCCCAATTTCGGGAAAGACTGTACCCCGAAAAACCCGGCAGGC Methanosarcinales-Methanosarci 983 CAAAGCCTTCCC-AGTGTCCAAACTTCGGTAAAGAATGTACCCCGAAAAACCCTGCAGGC Clostridia-Thermoanaerobacter_ 947 TATATCCAAATG-AATGTCCGCTATTTGGTAGAGTCTGCACCCCAGACAATCCGGTTGGG clostridia-clostridium_cell 971 AACGTCCTGGAG-ACTGCAAGCTTTTCAAAAAAAGATGTACTCCCGAAACTCCGGTAGGT cyano-crocosphaera_wat 977 AAAAACCTAACG-ATTGTCCTGCTTTCGGAACCCAATGTACACCAGAAAACCCTCTAGGT Bacteroides_Flavobacteriu_john 965 AAAGACCAAACG-AATGTCCTGAATTCGGAAAAAAATGCAAACCTTCTTTTCCGTTAGGA

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Proteo-others-MagentococcusMC1 1030 CCCTGTATGGCCTCGGTGGATGGGGCTTGCCGCATCATGTGGGAACGCCAACATAGTGA- beta-Nitrospira_multi 1027 CCGTGCATGGTGTCGGACGAAGGTGCTTGCCGCATCTGGTGGGCA-GCAGGCGTACGGGA beta-Thiobacillus_denitri 1024 CCGTGCATGGTCTCGGACGAAGGCGCCTGCCGCATCTGGTGGGCC-GGTGGCGTGCGCGT alpha-Acidiphilum 1030 CCCTGCATGGTCTCGGACGAAGGCGCCTGCCACATCTGGTGGCAG-GCGGGCATTCGGG- Thermococcales-Pyrococcus_fur 1042 CCTTGTATGGTTTCGTACGAAGGAACTTGTCACATATTTTACAAATATGGCGCC-CTGAT Thermococcales-Pyrococcus_hori 1042 CCCTGCATGGTTTCATATGAGGGAACTTGTCACATATTCTATAAGTATGGAGCG-CTGTT Thermococcales-Thermococcus_ko 1057 CCGTGCATGGTGTCCTACGAAGGAACATGCCAGATATTCTACAAGTACGGCGTG-CTGTT Archaeoglobales-Archaeoglobus 1048 GCGTGCATGGTGAGTGTGGAGGGGACGTGCAACATCTGGGCTACCTCCGGCACATCTCGT Methanopyrales-Methanopyrus_ka 988 CCGTGCATGGTGTCGGAGGAAGGCCCGTGTTTCATAGAGTACAGGTTCGGCGGA--GGGT Methanomicrobiales-Methanospir 964 CCGTGTATGGTCAGTCATGAAGGAGCGTGCAAGATTTGGCACCATTACCACATTCGGAAA Sulfolobales-Metallosphaera_se 1045 GCTCCAATGGTATCACAGGAAGGAACCTGTTGGATCTGGGCAACGCATAGAATTGTTAAT Thermoproteales-Pyrobaculum_is 1102 CCCTGTATGGTTTCTGTAGAGGGGACGTGTGCCATATGGGCGAGATTTGGAGGCGGAGGT Methanobacteriales-Methanother 988 GCATGCATGGTGTCAAGGGAGGGCACCTGTAACATAGCCTACCGGTACAGCTCATTCTAG Methanomicrobiales-Methanocull 967 CCCTGCATGGTCAGCCACGAAGGGGCGTGCAAGATATGGCATCTCTACGAATCGCGGAGG Thermoproteales-Thermofilum_pe 1126 CCCTGCATGGTTTCCCTCGAGGGGACGTGCGCCGTCTGGGCTAGGTTCGGAG-GGGGAGG delta-Anaeromyxobacter 1057 GCGTGCATGGTGTCGTCGGAGGGGCAGTGCCGGATCTGGCACACCTACGGCGGCGTGCCC delta-desulfovibrio_des 1030 CCGTGCATGGTTTCCACCGAAGGAAGCTGCGCCGCCTATTTCAAATACAATATCGGGAGC delta-geobacter_metalli 1036 GCCTGCATGGTCTCCTCCGAGGGGACCTGCGCGGCGGCGTACAAGTATGGGCAGTGA--- delta-Pelobacter_propion 1036 GCCTGCATGGTGTCCAGCGAAGGGACCTGCGCCGCGGCGTACAAGTACGGCAGATAG--- delta-geobacterFRC32 1036 CCGTGTATGGTCTCCTCCGAAGGCAGCTGCGCCGCGTATTATAACCATTAA--------- proteo-others-Mariprofundus_fe 1039 GCCTGCATGGTCTCCTCCGAAGGGGCGTGCGCCGCCTACTACAGCTACGGCAGATACCGT alpha-Stappia_agg 1057 GCGTGCATGGTCTCTTCGGAAGGCGCTTGCGCCGCCTACTGGAGCTATGGCCGCAATCGC beta-Methylibium_petro 1057 TCCTGCATGGTGTCCAGCGAGGGCGCCTGCGCGGCGCACTACTCCTACGGGCGGTTCAAG beta-Ralstonia_metall 1057 TCCTGCATGGTGTCGAGCGAAGGGGCGTGCGCGGCGCATTACAGCTACGGGCGGTTCAAG beta-Rhodoferax_ferri 1057 TCGTGCATGGTCAGCAGCGAAGGTGCCTGCGCGGCGCATTACAGTTACGGGCGTTACAAG beta-dechloromonas_aro 1057 TCCTGCATGGTCAGCTCCGAAGGCGCCTGTGCCGCTCACTACACTTATGGACGCTACAAG beta-Azoarcus 1060 TCGTGCATGGTGTCCTCCGAAGGCGCCTGCGCCGCCCACTACAATTACGGCCGTTTCCGC beta-Burkholderia-viet 1054 TCGTGCATGGTGTCGAGCGAGGGCGCGTGCGCCGCGCATTACTCGTATGGCCGTTTCAAG alpha-Magnetospirillum_mag 1057 TCGTGCATGGTCTCGGCCGAAGGCGCCTGCGCCGCCCATTGGACCTACGGCCGCTTCCGC alpha-Bradyrhizobium_jap 1066 GCCTGCATGGTGTCATCAGAAGGAGCTTGTGCGGCGTATTGGACCTATCGCCGAGTTCGC alpha-BradyrhizobiumBTAi1 1042 TCCTGCATGGTCTCGTCGGAAGGCGCCTGCGCGGCACATTGGACCTATGGCCGCTTCCGC alpha-Rhodopseudomonas_palBisA 1057 TCCTGCATGGTGTCGTCGGAAGGCGCCTGCGCCGCGCATTGGACCTACGGCCGGTTTCGC alpha-Rhodopseudomonas_palCGA0 1057 TCTTGCATGGTGTCTTCGGAAGGCGCCTGCGCAGCGCATTGGACCTACGGTCGCTTCCGC beta-Burkholderia_phy 1039 GCTTGCATGGTTTCGAGCGAAGGTGCCTGCGCAGCACACTACCAGTATGCCCGCTTCAAC alpha-Rhodospirillum_rub11170 1069 GCCTGCATGGTTTCGTCCGAGGGCGCCTGCGCCGCGGCTTACGCCTATGGCGGTCGCCGC beta-Burkholderia_xeno 1060 GCCTTGATGGTATCGAGCGAAGGTGCGTGCGCGGCGCACTATCGCTACGCACGCATCGAT alpha-Sphingopyxis_alask 1051 GCCCTGATGGTTTCGTCTGAAGGAGCATGTGCCGCCTATTATCAGTATGGCGGGATCGAT gamma-Shigella_flex 1051 GCACTGATGGTCTCCTCCGAAGGAGCGTGCGCCGCGTGGTATCAGTATCGTCAGCAGGAG gamma-Shigella_sonn 1051 GCACTGATGGTCTCCTCCGAAGGAGCGTGCGCCGCGTGGTATCAGTATCGTCAGCAGGAG gamma-Shigela_dys 1051 GCACTGATGGTCTCCTCCGAAGGAGCGTGCGCCGCGTGGTATCAGTATCGTCAGCAGGAG gamma-EcoliK12 1051 GCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGTGGTATCAGTATCGTCAGCAGGAG gamma-Ecoli_CFT073 1051 GCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGTGGTATCAGTATCGTCAGCAGGAG gamma-salmonella_ent 1051 GCGCTGATGGTCTCTTCCGAAGGCGCATGCGCCGCCTGGTATCAGTATCGTCAGCAGGAG gamma-salmonella_LT2 1048 GCGCTGATGGTCTCTTCCGAAGGCGCATGCGCCGCCTGGTATCAGTATCGTCAGCAGGAG gamma-enterobacter_638 1051 GCGCTGATGGTCTCTTCCGAAGGCGCCTGCGCCGCATGGTATCAGTATCGCAATCAGGAG gamma-erwinia_caro 1051 GCGCTGATGGTTTCTTCCGAGGGCGCGTGCGCCGCCTGGTATCAATATCGCGCACAGGAG gamma-Yersinia_ber 1051 GCACTGATGGTGTCATCCGAAGGGGCCTGCGCCGCTTATTATCAGTATCGATAA------ gamma-Yersinia_int 1051 GCACTGATGGTGTCATCCGAAGGGGCCTGCGCCGCTTATTATCAGTATCGATAA------ gamma-Yersinia_fred 1051 GCGCTGATGGTTTCCTCTGAGGGAGCTTGCTCCGCTTACTACCAATATAAATAA------ gamma-Yersinia_moll 1051 GCGCTGATGGTTTCTTCCGAGGGAGCCTGTTCAGCCTATTATTTGTACCGATAG------ gamma-Serratia_protea 1051 GCGTTGATGGTGTCTTCGGAAGGTGCCTGCGCCGCCTGGTACCAATACCGTCGTGAAACA alpha-Rhodobacter_sphaer 1063 GCGCTGATGGTGAGTTCGGAAGGCGCCTGCGCGGCCTACTGGCAATATGCCGGCGCGCGC alpha-Rhodobacter_spaer17025 1063 GCGCTGATGGTGTCCTCCGAGGGCGCATGCGCGGCCTACTGGCAGTATGGCGGCGCGCGG alpha-Roseovarius-2601 1063 GCGTTGATGGTGTCGTCCGAGGGGGCTTGCGCCGCCTACTGGACCTATGGCGGCGCGCGC alpha-Paracoccus_deni 1063 GCGCTGATGGTCAGTTCGGAAGGGGCCTGTGCGGCCTATTGGCAATATGGCGGTGCGC-- alpha-Xanhobacter_auto 1051 GCGCTCATGGTGTCGTCCGAAGGCTCTTGCGCCGCCTATTACCAATATGGTGGCGTGCGG actinobacteria-corynebacterium 1030 ACGTGCATGGTATCGCCCGAAGGCGCCTGCGCTGCATATTACAACTTCGGACGTATCAAC Actinobacteria-RhodococcusRHA1 1033 ACGTGCATGGTGTCGCCCGAGGGCGCGTGCGCCGCGTACTACAACTTCGGCCGGCTCCAC Chloroflexi-Acidobacteria 1027 GCGACAATGGTCTCGAATGAAGGCGCCTGTGCTGCGTACTACCAATACCGGCGGCACGAA Chloroflexi-Solibacter_usi 1027 GCGACGATGGTGTCGGCGGAGGGCGCGTGCGCGGCGTACTACAACTACGGGCGGCATCTG delta-proteo_MMS1 1159 GCCACCATGGTTTCCCGGGAGGGGGCCTGCCACGCCTATTACCGG-GCCAATCGGAG--G Cyan-SynechococcusWH5701 1042 GCCCCGATGGTGTCGGCGGAAGGGGCCTGCGCCGCCTACTGGCGCTACCGCTCCCGG--A Cyan-Synechocystis6803 1036 GCTCCCATGGTTTCCTCGGAAGGAGCCTGTGCCGCCTATTACCGTTATCGCCAACAATTA chloroflexi-chloroflexus_agg 1027 GCAACGATGGTATCAAGCGAGGGAGCATGCGCAGCGTATTATCAGTATGGCCGATTCGTG chloroflexi-chloroflexus_aur 1027 GCGACCATGGTCTCAAGTGAAGGCGCCTGCGCGGCGTACTACCAGTACGGACGCTTCATT Chloroflexi-Roseiflexus_cas 1027 GCAACGATGGTGTCGAGCGAAGGGGCATGCGCTGCGTACTATCAGTACGGACGGTTCATC Chloroflexi-RoseiflexusRS-1 1027 GCGACGATGGTGTCGAGCGAAGGCGCATGCGCAGCCTACTACCAGTACGGACGCTTCATC Actinobacteria-Mycobacterium_g 1027 GCCACCATGGTCTCCTCCGAAGGCGCCTGTGCGGCGTACTACCTCTACCGGCGGCTGGAG Actinobacteria-Mycobacterium_v 1027 GCGACCATGGTTTCGTCGGAGGGCGCGTGCGCGGCCTACTACCTGTACCGTCGACTCGAG Actinobacteria-FrankiaCcI3 1027 GCGACCATGGTGTCCTCCGAAGGGGCCTGTGCCGCCTACTACCTCTACCGCCGGATGAGC Actinobacteria-FrankiaACN14a 1027 GCGACGATGGTGTCCGCCGAGGGTGCCTGCGCGGCCTACTACCTCTACCGGCGGCTCGGC Actinobacteria-Streptomyces_av 1027 GCCACCATGGTCTCCAGCGAGGGCGCCTGCGCCGCGTACTACCTCTACCGGCGACTGGAC Actinobacteria-Mycobacterium_s 1027 GCCACCATGGTGTCGAGCGAAGGCGCGTGCGCCGCGTACTACCAGTTCCGTCGACTCGAG Chlorobi-Pelodictyon_lut 1027 GCGACAATGGTTTCTTCAGAAGGGGCATGTGCCGCATACTACAACTATCACCGCAACCTC

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Chlorobi-Pelodicton_lut 1027 GCCACCATGGTCTCCTCCGAAGGGGCCTGTGCTGCCTATTACAACTATCACCGAAATTTC chlorobi-chlorobium_lim 1027 GCGACGATGGTTTCTTCCGAAGGCGCCTGTGCGGCATATTACCGGTATCATCGAAATTCC Chlorobi-Prosthecochloris_aest 1027 GCCCCGATGGTATCGGGAGAGGGCGCTTGTGCGGCATACTATCGATATCAGCGGGCCTGG chlorobi-chlorobium_tep 1027 GCAACCATGGTTTCCAGCGAAGGCGCCTGCGCCGCGTATTATCGGTATCACCGCAACAGT delta-Syntrophus_acid 1036 CCCTGCATGGTGTCCAGCGAGGGGACCTGCGCGGCATACCTGAAATATGAAATCCATAAC delta-desulfotalea_psy 1027 CCCTGCATGGTTAGTAGCGAAGGCACATGTGCCGCCTATTACAAATATGGCCAGGACCTG chloroflexi-dehalococcoides_et 1030 CCGTGCATGGTCTCTTCCGAGGGCACCTGCGCCGCCTATTACCACTACGGAGGC------ chloroflexi-dehalococcoides_CB 1030 CCGTGCATGGTCTCCTCTGAGGGCACTTGTGCCGCCTATTACCATTACGGAGGC------ clostridia-desulfitobacterium_ 1036 CCTTGCATGGTTTCCTCAGAAGGGTCTTGTGCAGCTTATTACCGATATGCGCAA------ Clostr-Moorella_therm 1054 CCCTGTATGGTTTCCAGTGAAGGGGCCTGCGCGGCTTACTATCGATTTGAACGTTTTTCT Bacteroides-Robiginitalea_bif 1027 GCCCCCATGGTCAGTTCAGAGGGAGCTTGTGCCGCATACTATCACTTCAGTGGAGCCGCA epsilon-Helicobactr_acin 1054 AGTTGCATGGTCAGCTCTGAGGGGGCGTGTTCGGCGTATTATCGTTA---CAAG-CGCGT epsilon-Helicobacter_pylor 1054 AGTTGCATGGTCAGCTCTGAGGGGGCGTGCGCGGCGTATTATCGTTA---CAAG-CGCGT gamma-PhotobacteriumSKA34 1060 AGCTGTATGGTGAGCTCTGAAGGTGCATGTAATGCTTATTTCCGTTA--CCGA--GGTGT gamma-Vibrio_ang 1060 AGTTGTATGGTGAGTTCTGAAGGTGCTTGTAATGCTTATTTCCGTTA--CCGA--GGCGT Gamma-Photobacterium_prof 1057 AGCTGTATGGTGAGTTCTGAAGGTGCTTGCAATGCTTATTACCGCTATGCTGACCGATGC epsilon-Wolinella_succ 1054 AGCTGTATGGTGAGTAGCGAGGGGGCGTGTGCGGCCTATTACAAATATGGTCGGGAGCAC epsilon-campylobacer_con 1027 TCGTGCATGGTCTCAAGCGAGGGCGCTTGTGCTGCATATTTTAAATAC-GCAAGAGTTGG Aquifex 1075 TCCTGTATGGTTTCTTCGGAAGGAGCCTGTGCGGCTTACTACAAGTA---CAGAAGGGCT gamma-Actinobacillus 1054 GCATTAATGGTTTCTTCTGAGGGGGCTTGTTCGGCTTACTATCAATATCGGAGAGAAATG gamma-Manheimia-succ 1054 GCATTGATGGTTTCTTCGGAAGGTGCCTGTGCGGCTTATTATCAGTATCGCAGAGA---G gamma-Acidobacillus_succinogen 1054 GCATTAATGGTGTCATCCGAAGGCGCTTGCGCCGCATATTATCAATACCGACGGGA---A Cyano-Avar 1039 ACCTGCATGGTGTCCTCAGAAGGAGCTTGTGCCGCATACTATAAATATGGCAGATTTTCG Cyan-Nostoc7120 1039 ACTTGCATGGTGTCCTCAGAAGGAGCTTGTGCAGCATACTATAAATATGGCAGATTTTCG Cyan-Nodularia_spu 1039 AGCTGTATGGTTTCCTCAGAAGGTGCGTGTGCAGCCTACTACAAATATGGACGACTTTCT Cyan-Lyngbya8106 1051 ACTTGCATGGTTTCTTCAGAAGGGGCTTGTGCAGCTTATTACAAATATGGTCGCCTTTCA Cano-trichodesmium_ery 1051 ACTTGCATGGTTTCCTCTGAAGGTGCTTGTGCTGCCTATTATAAATATGGTCGGTTGTTT clostridia-caldicelluosiruptor 979 GCGTGTATGGTATCTCAGGAAGGGTCGTGTAATGCATATTTTAGGTAC-GGGAAGTGGAA clostridia-clostridium_beij 1018 CCTTGTATGGTATCTACGGAGGGATCTTGCTCTATTTTTTATAAGTATAGGGGAGTGGAA Methanobacteriales-Methanospha 985 GCATGTATGGTAAGTAAAGAAGGTACTTGTAATATAGCATATAGATACTCAAAAATGGAT Thermococcales-Pyrococcus_abys 985 GCATGTATGGTAAGTAAAGAAGGTACTTGTAATATAGCATATAGATACTCAAAAATGGAT Methanococcales-Methanocaldoco 1018 AGTTGTATGGTTTCAGATGAGGGAACGTGTAGGATATTTTATAAGTATAGGAGGATTTAA Methanococcales-Methanococcus_ 1021 AGCTGCATGGTTTCTGACGAAGGAACTTGCAGAATCTTTTACAAATACAGCAAATTCAGA delta-Lawsonia_intra 1030 CCCTGTATGGTATCAACAGAAGGAAGTTGTGCAGCATACTTTAAATATCATATGATAAGA epsilon-campylobacter_jej 1027 AGTTGTATGGTTTCTGGCGAGGGTGCTTGTGCGGCGTATTATAAATACTCAAAGGTGAAT epsilon-campylobacter_ups 1027 AGTTGTATGGTTTCGGGTGAGGGGGCTTGTGCGGCGTATTACAAATACGCTAAAGCGCAT clostridia-clostridium_therm 991 GCATGCATGGTTTCTTCAGAGGGCGCCTGCGGAATCTGGTACAGAAATTGTGAGGTGTAG clostridia-carboxidothermus_hy 1018 GCTTGTATGGTATCCAGCGAAGGTAGTTGTGCAGCTTATTATAAATACGGTAGGAGGTAG Clostr-Halothermothrix_oren 1030 CCGTGTATGGTTTCCCGGGAAGGGACCTGTGCTGCATATTATCGATATCGGGGGAAGGTG Methanosarcinales-Methanosarci 1042 CCCTGCATGGTCAGCCAGGAGGGCATGTGCTACAACTGGTTCAGGTACTCACGGGAAGGG Methanosarcinales-Methanosarci 1042 CCCTGTATGGTAAGCCAGGAAGGTATGTGTTATAACTGGTTCAGGTACTCAAGAGAAGGA Clostridia-Thermoanaerobacter_ 1006 CCTTGCATGGTGTCTTCGGAAGGGTCCTGTGCAGCGTTTTACAAGTATGGCGCATAA--- clostridia-clostridium_cell 1030 TCATGTATGGTTTCTTCTGAAGGGACCTGTGCCGCGTACTACAAATATGGAGAATAA--- cyano-crocosphaera_wat 1036 GCGCCCATGGTATCTTCAGAAGGAGCTTGTGCTGCTTATTACCGCTATCGAAACCATCGG Bacteroides_Flavobacteriu_john 1024 GCGCCAATGGTTTCGTCTGAAGGTGCTTGTGCCGCTTATTTCAACTATCTATAA------

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Proteo-others-MagentococcusMC1 1089 -------GCTATTGGATTAG---------------------------------------- beta-Nitrospira_multi 1086 GAACACGGCCACTGGAGTGAAGACGGTGGCAGATAGCTCTTCTATTCCGGTGTTACCTGA beta-Thiobacillus_denitri 1083 CGCGGCCGATGCCTGA-------------------------------------------- alpha-Acidiphilum 1088 -------CGCACTGA--------------------------------------------- Thermococcales-Pyrococcus_fur 1101 GTAG-------------------------------------------------------- Thermococcales-Pyrococcus_hori 1101 TTGA-------------------------------------------------------- Thermococcales-Thermococcus_ko 1116 CTGA-------------------------------------------------------- Archaeoglobales-Archaeoglobus 1108 ACGCAATGA--------------------------------------------------- Methanopyrales-Methanopyrus_ka 1046 GA---------------------------------------------------------- Methanomicrobiales-Methanospir 1024 GTTTAA------------------------------------------------------ Sulfolobales-Metallosphaera_se 1105 GTTAGCCCTAGATGTAGGAGGTAA------------------------------------ Thermoproteales-Pyrobaculum_is 1162 -CTCGCCGTGGAGGTTGCAAAAGAGATAGGCCTGGCGTAA-------------------- Methanobacteriales-Methanother 1048 ------------------------------------------------------------ Methanomicrobiales-Methanocull 1027 GCATGA------------------------------------------------------ Thermoproteales-Thermofilum_pe 1185 GCTGGCGGACCAGATAGCTCGAGACCTAGCGCTGGAAGCCGGGCTGGGATAG-------- delta-Anaeromyxobacter 1117 GATCTGCGCAAGGTCGGCTAG--------------------------------------- delta-desulfovibrio_des 1090 TGA--------------------------------------------------------- delta-geobacter_metalli ------------------------------------------------------------ delta-Pelobacter_propion ------------------------------------------------------------ delta-geobacterFRC32 ------------------------------------------------------------ proteo-others-Mariprofundus_fe 1099 GAAACAACGGGCACAACATGA--------------------------------------- alpha-Stappia_agg 1117 GAGGCGGTTGCCGCCGCCAAGGGCGCTGCAGCCTGA------------------------ beta-Methylibium_petro 1117 GACATCGCGATCGTGGCGGCAGCATGA--------------------------------- beta-Ralstonia_metall 1117 GACATCGAAGTGGTGGTGGCAGCTTGA--------------------------------- beta-Rhodoferax_ferri 1117 GACAT-GACCCCATGA-------------------------------------------- beta-dechloromonas_aro 1117 GATGTCTGA--------------------------------------------------- beta-Azoarcus 1120 GACATCGCGGTGGTGGCCGCCTGA------------------------------------ beta-Burkholderia-viet 1114 GACATTCCGCTGGTACCCGCATGA------------------------------------ alpha-Magnetospirillum_mag 1117 GACCTGGAGACGAGCCATGCCTGA------------------------------------ alpha-Bradyrhizobium_jap 1126 GATGAGCAGGCGAGGCAGACGTCATGA--------------------------------- alpha-BradyrhizobiumBTAi1 1102 GACCATCAGCGGAGGCTCGCATCATGA--------------------------------- alpha-Rhodopseudomonas_palBisA 1117 GACCACGCGCGGAGGGCGGTGGCATGA--------------------------------- alpha-Rhodopseudomonas_palCGA0 1117 GACCACGCACGGAGGGCGGTGGCATGA--------------------------------- beta-Burkholderia_phy 1099 GATGCAGCTGCAGCCGGATGTGTTCGCCTATGA--------------------------- alpha-Rhodospirillum_rub11170 1129 GCCGCCACGCCTTTACGGAGACCCCAGCCATGA--------------------------- beta-Burkholderia_xeno 1120 ACATCGGCGCTGACCGCGGCAGCTGCTGTTTGTCCTGGACCCGCTCCGGCGGCGCTGCGA alpha-Sphingopyxis_alask 1111 GCGGTGCCGGAGCCAGCG-CAATGA----------------------------------- gamma-Shigella_flex 1111 AGTGAAGCGTGA------------------------------------------------ gamma-Shigella_sonn 1111 AATGAAGCGTGA------------------------------------------------ gamma-Shigela_dys 1111 AGTGAAGCGTGA------------------------------------------------ gamma-EcoliK12 1111 AGTGAAGCGTGA------------------------------------------------ gamma-Ecoli_CFT073 1111 AGTGAAGCGTGA------------------------------------------------ gamma-salmonella_ent 1111 TGTGAAGTTTGA------------------------------------------------ gamma-salmonella_LT2 1108 TGTGAAGTTTGA------------------------------------------------ gamma-enterobacter_638 1111 TGTGAAGCATGA------------------------------------------------ gamma-erwinia_caro 1111 ACTG---CATGA------------------------------------------------ gamma-Yersinia_ber ------------------------------------------------------------ gamma-Yersinia_int ------------------------------------------------------------ gamma-Yersinia_fred ------------------------------------------------------------ gamma-Yersinia_moll ------------------------------------------------------------ gamma-Serratia_protea 1111 GAGGAAACCCCGGCATGA------------------------------------------ alpha-Rhodobacter_sphaer 1123 GGGCTGG---CCGCGGAATAG--------------------------------------- alpha-Rhodobacter_spaer17025 1123 GGGCTGG---CCGCCGAATAG--------------------------------------- alpha-Roseovarius-2601 1123 GTGGTGGAGCCGGCGGAATGA--------------------------------------- alpha-Paracoccus_deni 1121 GGGTTG----CAGCGGAATAG--------------------------------------- alpha-Xanhobacter_auto 1111 GGAGAAG---CCGCATGA------------------------------------------ actinobacteria-corynebacterium 1090 CGTGAGGAGGCCGCGGCGCTACGCTAG--------------------------------- Actinobacteria-RhodococcusRHA1 1093 CGCGAGACGGCGCAATTGCTGGGTCAGCGAGGCTGA------------------------ Chloroflexi-Acidobacteria 1087 GCTAAGGCCGCGGTCGGGAGCGAACGATGA------------------------------ Chloroflexi-Solibacter_usi 1087 GTATCGATCGCAG-CGGAGGCGTGA----------------------------------- delta-proteo_MMS1 1216 GCGGCGGGATGA------------------------------------------------ Cyan-SynechococcusWH5701 1100 GCTGA------------------------------------------------------- Cyan-Synechocystis6803 1096 CCGGAACCAGTGGGAGCGGCCAGAGTTTAG------------------------------ chloroflexi-chloroflexus_agg 1087 CCAACCAGCACGATTGGTGTAGCATCGTAA------------------------------ chloroflexi-chloroflexus_aur 1087 CCGGTTAACCAGGTTGGCGTGACCGGCTAA------------------------------ Chloroflexi-Roseiflexus_cas 1087 AAGGCTGAAGAGGTGGGAGTCGCGCGATGA------------------------------ Chloroflexi-RoseiflexusRS-1 1087 AAAGCTGAAGAGGTGGGAGTAGCGCGATGA------------------------------ Actinobacteria-Mycobacterium_g 1087 G---TCACCCATGCCTGA------------------------------------------ Actinobacteria-Mycobacterium_v 1087 G---TGACCAATGCCTGA------------------------------------------ Actinobacteria-FrankiaCcI3 1087 ACC-TCCCCGGCGATTGCGCAGCCCCTGCCGGAAGCGGGACGTGCGCCGGAAGCGGGACA Actinobacteria-FrankiaACN14a 1087 CAG-TCGACCACGACGGCCCCGGCGGCTGCTCCGACGGCTGCGACGGCGGCCCCGGCGGC Actinobacteria-Streptomyces_av 1087 ATCACCACCGCCGCCCGGGAGGCGAGCCCCGTTGTCTGA--------------------- Actinobacteria-Mycobacterium_s 1087 GCGGCCGCTCATGTCTGA------------------------------------------ Chlorobi-Pelodictyon_lut 1087 TGA---------------------------------------------------------

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Chlorobi-Pelodicton_lut 1087 GCACTATGA--------------------------------------------------- chlorobi-chlorobium_lim 1087 GAACTCTTATGA------------------------------------------------ Chlorobi-Prosthecochloris_aest 1087 GGAGCCTGA--------------------------------------------------- chlorobi-chlorobium_tep 1087 AACACTTGA--------------------------------------------------- delta-Syntrophus_acid 1096 CGAAAATCGTAA------------------------------------------------ delta-desulfotalea_psy 1087 TAG--------------------------------------------------------- chloroflexi-dehalococcoides_et 1084 -------AAACTTGGATAA----------------------------------------- chloroflexi-dehalococcoides_CB 1084 -------AAACTTGGATAA----------------------------------------- clostridia-desulfitobacterium_ 1090 ------AGGAGTGGGATATAA--------------------------------------- Clostr-Moorella_therm 1114 CCAGTTGGAGGTGGCATTTAG--------------------------------------- Bacteroides-Robiginitalea_bif 1087 GAAACCGTTGCATAA--------------------------------------------- epsilon-Helicobactr_acin 1110 TTGA-------------------------------------------------------- epsilon-Helicobacter_pylor 1110 TTGA-------------------------------------------------------- gamma-PhotobacteriumSKA34 1116 TTAA-------------------------------------------------------- gamma-Vibrio_ang 1116 TTAA-------------------------------------------------------- Gamma-Photobacterium_prof 1117 TAA--------------------------------------------------------- epsilon-Wolinella_succ 1114 CTGAATTAA--------------------------------------------------- epsilon-campylobacer_con 1086 TTAA-------------------------------------------------------- Aquifex 1132 TTAGTTCAGTAA------------------------------------------------ gamma-Actinobacillus 1114 TAA--------------------------------------------------------- gamma-Manheimia-succ 1111 TAA--------------------------------------------------------- gamma-Acidobacillus_succinogen 1111 TAA--------------------------------------------------------- Cyano-Avar 1099 ACAACGTTACAAAAACAAGCAGCCGAAAAGCCAAAAGTGACTATCTCGTCATAA------ Cyan-Nostoc7120 1099 ACAACGTTGCAAAAACAAGCAGCCGAAAAGCCAAAAGTGACTATCTCGTCATAA------ Cyan-Nodularia_spu 1099 GCAA--TTAGTAAAAAAGCCCAACAACTAGCCACATCCAAATAA---------------- Cyan-Lyngbya8106 1111 ACCGTTATAAAAGCTAACAAACCCCAACGTTCTAAAGCAAAAGTCTAA------------ Cano-trichodesmium_ery 1111 AAGAAGGAAAAAGTTAA---GGTTTAA--------------------------------- clostridia-caldicelluosiruptor 1038 AGGAAGATGA-------------------------------------------------- clostridia-clostridium_beij 1078 TAA--------------------------------------------------------- Methanobacteriales-Methanospha 1045 GATTAA------------------------------------------------------ Thermococcales-Pyrococcus_abys 1045 GATTAA------------------------------------------------------ Methanococcales-Methanocaldoco 1078 ------------------------------------------------------------ Methanococcales-Methanococcus_ 1081 TAA--------------------------------------------------------- delta-Lawsonia_intra 1090 TAA--------------------------------------------------------- epsilon-campylobacter_jej 1087 GCATGA------------------------------------------------------ epsilon-campylobacter_ups 1087 TAA--------------------------------------------------------- clostridia-clostridium_therm 1051 ------------------------------------------------------------ clostridia-carboxidothermus_hy 1078 ------------------------------------------------------------ Clostr-Halothermothrix_oren 1090 AATTTATAA--------------------------------------------------- Methanosarcinales-Methanosarci 1102 GGAAGCAGGTTTGCATGA------------------------------------------ Methanosarcinales-Methanosarci 1102 GGCAACAGAGTTGCATGA------------------------------------------ Clostridia-Thermoanaerobacter_ ------------------------------------------------------------ clostridia-clostridium_cell ------------------------------------------------------------ cyano-crocosphaera_wat 1096 CAACTGGTGGGGCAATATTAA--------------------------------------- Bacteroides_Flavobacteriu_john ------------------------------------------------------------

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Proteo-others-MagentococcusMC1 ------------------------------------------------------------ beta-Nitrospira_multi 1146 GAAGCCAGAATAA----------------------------------------------- beta-Thiobacillus_denitri ------------------------------------------------------------ alpha-Acidiphilum ------------------------------------------------------------ Thermococcales-Pyrococcus_fur ------------------------------------------------------------ Thermococcales-Pyrococcus_hori ------------------------------------------------------------ Thermococcales-Thermococcus_ko ------------------------------------------------------------ Archaeoglobales-Archaeoglobus ------------------------------------------------------------ Methanopyrales-Methanopyrus_ka ------------------------------------------------------------ Methanomicrobiales-Methanospir ------------------------------------------------------------ Sulfolobales-Metallosphaera_se ------------------------------------------------------------ Thermoproteales-Pyrobaculum_is ------------------------------------------------------------ Methanobacteriales-Methanother ------------------------------------------------------------ Methanomicrobiales-Methanocull ------------------------------------------------------------ Thermoproteales-Thermofilum_pe ------------------------------------------------------------ delta-Anaeromyxobacter ------------------------------------------------------------ delta-desulfovibrio_des ------------------------------------------------------------ delta-geobacter_metalli ------------------------------------------------------------ delta-Pelobacter_propion ------------------------------------------------------------ delta-geobacterFRC32 ------------------------------------------------------------ proteo-others-Mariprofundus_fe ------------------------------------------------------------ alpha-Stappia_agg ------------------------------------------------------------ beta-Methylibium_petro ------------------------------------------------------------ beta-Ralstonia_metall ------------------------------------------------------------ beta-Rhodoferax_ferri ------------------------------------------------------------ beta-dechloromonas_aro ------------------------------------------------------------ beta-Azoarcus ------------------------------------------------------------ beta-Burkholderia-viet ------------------------------------------------------------ alpha-Magnetospirillum_mag ------------------------------------------------------------ alpha-Bradyrhizobium_jap ------------------------------------------------------------ alpha-BradyrhizobiumBTAi1 ------------------------------------------------------------ alpha-Rhodopseudomonas_palBisA ------------------------------------------------------------ alpha-Rhodopseudomonas_palCGA0 ------------------------------------------------------------ beta-Burkholderia_phy ------------------------------------------------------------ alpha-Rhodospirillum_rub11170 ------------------------------------------------------------ beta-Burkholderia_xeno 1180 GTGCAAGCATGA------------------------------------------------ alpha-Sphingopyxis_alask ------------------------------------------------------------ gamma-Shigella_flex ------------------------------------------------------------ gamma-Shigella_sonn ------------------------------------------------------------ gamma-Shigela_dys ------------------------------------------------------------ gamma-EcoliK12 ------------------------------------------------------------ gamma-Ecoli_CFT073 ------------------------------------------------------------ gamma-salmonella_ent ------------------------------------------------------------ gamma-salmonella_LT2 ------------------------------------------------------------ gamma-enterobacter_638 ------------------------------------------------------------ gamma-erwinia_caro ------------------------------------------------------------ gamma-Yersinia_ber ------------------------------------------------------------ gamma-Yersinia_int ------------------------------------------------------------ gamma-Yersinia_fred ------------------------------------------------------------ gamma-Yersinia_moll ------------------------------------------------------------ gamma-Serratia_protea ------------------------------------------------------------ alpha-Rhodobacter_sphaer ------------------------------------------------------------ alpha-Rhodobacter_spaer17025 ------------------------------------------------------------ alpha-Roseovarius-2601 ------------------------------------------------------------ alpha-Paracoccus_deni ------------------------------------------------------------ alpha-Xanhobacter_auto ------------------------------------------------------------ actinobacteria-corynebacterium ------------------------------------------------------------ Actinobacteria-RhodococcusRHA1 ------------------------------------------------------------ Chloroflexi-Acidobacteria ------------------------------------------------------------ Chloroflexi-Solibacter_usi ------------------------------------------------------------ delta-proteo_MMS1 ------------------------------------------------------------ Cyan-SynechococcusWH5701 ------------------------------------------------------------ Cyan-Synechocystis6803 ------------------------------------------------------------ chloroflexi-chloroflexus_agg ------------------------------------------------------------ chloroflexi-chloroflexus_aur ------------------------------------------------------------ Chloroflexi-Roseiflexus_cas ------------------------------------------------------------ Chloroflexi-RoseiflexusRS-1 ------------------------------------------------------------ Actinobacteria-Mycobacterium_g ------------------------------------------------------------ Actinobacteria-Mycobacterium_v ------------------------------------------------------------ Actinobacteria-FrankiaCcI3 1146 TGTGCTGGAGGTGGGACGTGCGCTGGAGGTGGGACGTGCGCTGGAGGTGGGACATGGTGG Actinobacteria-FrankiaACN14a 1146 CACGGCGGCCCCGGCGGCCACGTCGGCCTCGGCGGCCCCGGCGGACGTCCGGCCCGCGCG Actinobacteria-Streptomyces_av ------------------------------------------------------------ Actinobacteria-Mycobacterium_s ------------------------------------------------------------ Chlorobi-Pelodictyon_lut ------------------------------------------------------------

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Chlorobi-Pelodicton_lut ------------------------------------------------------------ chlorobi-chlorobium_lim ------------------------------------------------------------ Chlorobi-Prosthecochloris_aest ------------------------------------------------------------ chlorobi-chlorobium_tep ------------------------------------------------------------ delta-Syntrophus_acid ------------------------------------------------------------ delta-desulfotalea_psy ------------------------------------------------------------ chloroflexi-dehalococcoides_et ------------------------------------------------------------ chloroflexi-dehalococcoides_CB ------------------------------------------------------------ clostridia-desulfitobacterium_ ------------------------------------------------------------ Clostr-Moorella_therm ------------------------------------------------------------ Bacteroides-Robiginitalea_bif ------------------------------------------------------------ epsilon-Helicobactr_acin ------------------------------------------------------------ epsilon-Helicobacter_pylor ------------------------------------------------------------ gamma-PhotobacteriumSKA34 ------------------------------------------------------------ gamma-Vibrio_ang ------------------------------------------------------------ Gamma-Photobacterium_prof ------------------------------------------------------------ epsilon-Wolinella_succ ------------------------------------------------------------ epsilon-campylobacer_con ------------------------------------------------------------ Aquifex ------------------------------------------------------------ gamma-Actinobacillus ------------------------------------------------------------ gamma-Manheimia-succ ------------------------------------------------------------ gamma-Acidobacillus_succinogen ------------------------------------------------------------ Cyano-Avar ------------------------------------------------------------ Cyan-Nostoc7120 ------------------------------------------------------------ Cyan-Nodularia_spu ------------------------------------------------------------ Cyan-Lyngbya8106 ------------------------------------------------------------ Cano-trichodesmium_ery ------------------------------------------------------------ clostridia-caldicelluosiruptor ------------------------------------------------------------ clostridia-clostridium_beij ------------------------------------------------------------ Methanobacteriales-Methanospha ------------------------------------------------------------ Thermococcales-Pyrococcus_abys ------------------------------------------------------------ Methanococcales-Methanocaldoco ------------------------------------------------------------ Methanococcales-Methanococcus_ ------------------------------------------------------------ delta-Lawsonia_intra ------------------------------------------------------------ epsilon-campylobacter_jej ------------------------------------------------------------ epsilon-campylobacter_ups ------------------------------------------------------------ clostridia-clostridium_therm ------------------------------------------------------------ clostridia-carboxidothermus_hy ------------------------------------------------------------ Clostr-Halothermothrix_oren ------------------------------------------------------------ Methanosarcinales-Methanosarci ------------------------------------------------------------ Methanosarcinales-Methanosarci ------------------------------------------------------------ Clostridia-Thermoanaerobacter_ ------------------------------------------------------------ clostridia-clostridium_cell ------------------------------------------------------------ cyano-crocosphaera_wat ------------------------------------------------------------ Bacteroides_Flavobacteriu_john ------------------------------------------------------------

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Proteo-others-MagentococcusMC1 ------------------- beta-Nitrospira_multi ------------------- beta-Thiobacillus_denitri ------------------- alpha-Acidiphilum ------------------- Thermococcales-Pyrococcus_fur ------------------- Thermococcales-Pyrococcus_hori ------------------- Thermococcales-Thermococcus_ko ------------------- Archaeoglobales-Archaeoglobus ------------------- Methanopyrales-Methanopyrus_ka ------------------- Methanomicrobiales-Methanospir ------------------- Sulfolobales-Metallosphaera_se ------------------- Thermoproteales-Pyrobaculum_is ------------------- Methanobacteriales-Methanother ------------------- Methanomicrobiales-Methanocull ------------------- Thermoproteales-Thermofilum_pe ------------------- delta-Anaeromyxobacter ------------------- delta-desulfovibrio_des ------------------- delta-geobacter_metalli ------------------- delta-Pelobacter_propion ------------------- delta-geobacterFRC32 ------------------- proteo-others-Mariprofundus_fe ------------------- alpha-Stappia_agg ------------------- beta-Methylibium_petro ------------------- beta-Ralstonia_metall ------------------- beta-Rhodoferax_ferri ------------------- beta-dechloromonas_aro ------------------- beta-Azoarcus ------------------- beta-Burkholderia-viet ------------------- alpha-Magnetospirillum_mag ------------------- alpha-Bradyrhizobium_jap ------------------- alpha-BradyrhizobiumBTAi1 ------------------- alpha-Rhodopseudomonas_palBisA ------------------- alpha-Rhodopseudomonas_palCGA0 ------------------- beta-Burkholderia_phy ------------------- alpha-Rhodospirillum_rub11170 ------------------- beta-Burkholderia_xeno ------------------- alpha-Sphingopyxis_alask ------------------- gamma-Shigella_flex ------------------- gamma-Shigella_sonn ------------------- gamma-Shigela_dys ------------------- gamma-EcoliK12 ------------------- gamma-Ecoli_CFT073 ------------------- gamma-salmonella_ent ------------------- gamma-salmonella_LT2 ------------------- gamma-enterobacter_638 ------------------- gamma-erwinia_caro ------------------- gamma-Yersinia_ber ------------------- gamma-Yersinia_int ------------------- gamma-Yersinia_fred ------------------- gamma-Yersinia_moll ------------------- gamma-Serratia_protea ------------------- alpha-Rhodobacter_sphaer ------------------- alpha-Rhodobacter_spaer17025 ------------------- alpha-Roseovarius-2601 ------------------- alpha-Paracoccus_deni ------------------- alpha-Xanhobacter_auto ------------------- actinobacteria-corynebacterium ------------------- Actinobacteria-RhodococcusRHA1 ------------------- Chloroflexi-Acidobacteria ------------------- Chloroflexi-Solibacter_usi ------------------- delta-proteo_MMS1 ------------------- Cyan-SynechococcusWH5701 ------------------- Cyan-Synechocystis6803 ------------------- chloroflexi-chloroflexus_agg ------------------- chloroflexi-chloroflexus_aur ------------------- Chloroflexi-Roseiflexus_cas ------------------- Chloroflexi-RoseiflexusRS-1 ------------------- Actinobacteria-Mycobacterium_g ------------------- Actinobacteria-Mycobacterium_v ------------------- Actinobacteria-FrankiaCcI3 1206 ATGA--------------- Actinobacteria-FrankiaACN14a 1206 GGAGGTGGCCTGTGCCTGA Actinobacteria-Streptomyces_av ------------------- Actinobacteria-Mycobacterium_s ------------------- Chlorobi-Pelodictyon_lut -------------------

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Chlorobi-Pelodicton_lut ------------------- chlorobi-chlorobium_lim ------------------- Chlorobi-Prosthecochloris_aest ------------------- chlorobi-chlorobium_tep ------------------- delta-Syntrophus_acid ------------------- delta-desulfotalea_psy ------------------- chloroflexi-dehalococcoides_et ------------------- chloroflexi-dehalococcoides_CB ------------------- clostridia-desulfitobacterium_ ------------------- Clostr-Moorella_therm ------------------- Bacteroides-Robiginitalea_bif ------------------- epsilon-Helicobactr_acin ------------------- epsilon-Helicobacter_pylor ------------------- gamma-PhotobacteriumSKA34 ------------------- gamma-Vibrio_ang ------------------- Gamma-Photobacterium_prof ------------------- epsilon-Wolinella_succ ------------------- epsilon-campylobacer_con ------------------- Aquifex ------------------- gamma-Actinobacillus ------------------- gamma-Manheimia-succ ------------------- gamma-Acidobacillus_succinogen ------------------- Cyano-Avar ------------------- Cyan-Nostoc7120 ------------------- Cyan-Nodularia_spu ------------------- Cyan-Lyngbya8106 ------------------- Cano-trichodesmium_ery ------------------- clostridia-caldicelluosiruptor ------------------- clostridia-clostridium_beij ------------------- Methanobacteriales-Methanospha ------------------- Thermococcales-Pyrococcus_abys ------------------- Methanococcales-Methanocaldoco ------------------- Methanococcales-Methanococcus_ ------------------- delta-Lawsonia_intra ------------------- epsilon-campylobacter_jej ------------------- epsilon-campylobacter_ups ------------------- clostridia-clostridium_therm ------------------- clostridia-carboxidothermus_hy ------------------- Clostr-Halothermothrix_oren ------------------- Methanosarcinales-Methanosarci ------------------- Methanosarcinales-Methanosarci ------------------- Clostridia-Thermoanaerobacter_ ------------------- clostridia-clostridium_cell ------------------- cyano-crocosphaera_wat ------------------- Bacteroides_Flavobacteriu_john ------------------- !

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Supplemental material Selenter Westensee See · 2014. 5. 19. · ! 1 Supplemental material Fig. S1: 1.5 % Agarosegels of PCR-products of hypD of the samples taken from the two different