Supplemental Material, Quan et al. contains: Supplemental ... · Supplemental Material, Quan et al. contains: Supplemental Figures S1 to S7 Supplemental Document 1 and 2 Supplemental

Supplemental Material, Quan et al. contains:

Supplemental Figures S1 to S7

Supplemental Document 1 and 2

Supplemental Tables IX, X, XI, XV, XVI

Supplemental Figure S1

Supplemental Figure S1. ICL activity of Arabidopsis

seedlings after growing 3 to 6 days. Three technical repeats

were used. Error bars are standard deviations.

0

5

10

15

20

25

30

35

40

3d 4d 5d 6d

ICL a

ctivity (

U/m

g p

rote

in)


200 kDa

100

75

50

37

25

20

150

Supplemental Figure S2.

Silver-stained gel for the

immunoblot shown in Figure

1D. CE, crude extract; P1,

pellet fraction after the first

gradient centrifugation: PE,

highly enriched peroxisomes

after the second gradient

centrifugation. After Bradford

measurement of protein levels,

15 µg of proteins were loaded

in each lane.

CE P1 PE


Supplemental Figure S3. Subcellular distribution of proteins identified from

total peroxisomes (T1 and T2) in this study based on protein number.

T2 (n=135)

Cytosol: 7.4%

Nucleus: 3.7%

Mitochondria: 5.2%

Plastid: 40%

Secretory system: 11.9%

Plasma membrane: 0.7%

Peroxisome: 31.1%

T1(n=147)

Cytosol: 11.6%

Nucleus: 2%

Mitochondria: 4.8%

Plastid: 27.2%

Secretory system: 19.7%

Plasma membrane: 5.4%

Peroxisome: 29.3%

Supplemental

Figure S4

YFP-UP3 (SSL>) YFP-UP2 (SLN>) YFP-BADH (SKL>)

YFP-AAE5 (SRM>)

Overla

y

YF

P f

usio

n

CF

P-P

TS

1

YFP-SDRd (SSL>)

YFP-ATMS1 (SAK>)

A B C

YFP-GOX3 (AKL>)

Supplemental Figure S2. Verification of peroxisomal localization for six proteins identified from this study and our previous leaf

peroxisomal proteomics but had not been confirmed before.

Confocal microscopic images were taken from leaf epidermal cells of 4-week-old Nicotiana tabacum plants co-expressing YFP

fusions of the candidate proteins and the peroxisomal marker protein CFP-PTS1. Scale bars = 10 µm.

Overla

y

YF

P fusio

n

CF

P-P

TS

1

YFP-GOX3 (AKL>) E F G

8

9 20 16

59

10

Green leaf (Reumann et al., 2009): 105 Cell culture (Eubel et al., 2008): 84

Etiolated seedlings (This study): 77

Supplemental Figure S5. Comparison of the number of

peroxisomal proteins identified from different tissues from

different studies.



PXN

APX3

NDPK1

ASP3

PEX11e

HIT1

PEX11a

ACX2

GOX1/GOX2

PEX11d

OPR3

MDH2

RDL1

ATMS1

SCPL20

LACS6

ICL

ICDH

ECHIA

IBR10

PMDH1

MLS

CAT2

KAT2

4CL1

IBR1

CSD3

SO

SCP2

SDR

UP9

CHY1

BADH

BZO1

MFP2

LACS7

KAT1

AtDCI

CSY2

ACX4

ACX3

NQR

NS

GAPC2

st1

SDRc

UP3

6PGDH

GGT1

CuAO

HPR1

GOX3

PEX14

SDRb

Uri

ECH2

CSY3

PXA1/CTS

ATF2

ACH2

GSTT1

CAT3

st3

ACX1

ZnDH

AAE5

CAT1

HBCDH

AIM1

IBR3

ACAT1.3

LON2

UP6

AtHsp15.7

GGT2

1 2 3 4 5 6 7

-5 5 0

Supplemental Figure S6. Heatmaps showing relative expression levels of genes encoding peroxisomal

proteins identified from etiolated seedlings in this study. (A) Log2 normalized expression at various

developmental stages. 1, seedling cotyledons; 2, vegetative rosette; 3, flowering rosette; 4, flower stage 12;

5, senescing leaf; 6, seed6-silique; 7, seed10-silique; 8, embryo heart, cotyledon; 9, embryo torpedo,

cotyledon; 10, dry seeds; 11, 24h imbibed seed. (B) Log2 normalized expression during seed germination as

compared with dry seed. 1, fresh seeds; 2, 12h stratified; 3, 48h stratified; 4, 6h light grown; 5, 12h light

grown; 6, 24h light grown; 7, 48h light grown.

ICL MLS UP6 BZO1 LACS7 ECHIA CSY3 GOX3 AtHsp15.7 CAT1 GGT2 MDH1 PXA1 CSD3 UP9 IBR10 ACAT1.3 PEX11a KAT2 GSTT1 ACX1 AAE5 ACX3 ASP3 SCP2 LACS6 MFP2 ACX2 IBR3 CSY2 LON2 ZnDH RDL1 SDRb ATF2 SO HIT1 6PGDH NDPK1 sT1 Scpl20 GAPC2 NS ECH2 PEX11e ACX4 IBR1 PXN NQR ICDH PEX11d APX3 BADH ACH2 sT3 KAT1 UP3 SDRc SDRd CAT3 CAT2 HBCDH AIM1 PEX14 AtDCI 4CLP1 CHY1 Uri CuAO ATMS1 OPR3 GOX1/GOX2 MDH2 GGT1 HPR1

1 2 3 4 5 6 7 8 9 10 11

5 -5 10 0 -10

A B


Supplemental Figure S7. Phenotypic analysis of the mutants of the newly validated peroxisomal genes. (A) Seed

germination in response to ABA. Percentage of radicle emergence from seeds 6 days after growing on ½ LS

supplemented with the indicated concentrations of ABA was quantified. Each data point represents the mean (±SE)

of three independent experiments (n=100 per experiment). Mutants in this graph do not show statistically significant

difference from the wild type plant. (B) Sugar dependence assays. Hypocotyl lengths of 7d dark-grown seedlings on

1/2 LS supplemented with or without 1% sucrose are shown. Except for the pex14 control, no statistically significant

differences were found between the mutants and the wild type. (C) IBA response assays. Relative root length (on

IBA vs. w/o IBA) of 7d seedlings on 1/2 LS supplemented with 5 µM, 10 µM, or 15 µM of IBA are shown. Data

represent the mean (±SE) of three independent experiments. For each experiment, n=50. Asterisks indicate

measurements significantly different from the wild type (Student’s t test: one asterisk, p<0.05; two asterisks,

p<0.005). All experiments on gapc2-2 mutant were performed separately.

B

0.0

0.5

1.0

1.5

2.0

2.5

Col-0 Col-3 pex14 bzo1-4 scpl20-1 scpl20-2 gox3-1 rdl1-1 rdl1-2

+ sucrose

- sucrose

Hypo

co

tyl le

ng

th (

cm

)

**

0.0

0.5

1.0

1.5

2.0

2.5

Col-0 gapc2 pex14 Col-0 gapc2-2 pex14

**

gox3-1

0

20

40

60

80

100

120

Col-0 Col-3 pex14 bzo1-4 scpl20-1 scpl20-2 gox3-1

IBA 5µM IBA 10µM IBA 15µM C

Rela

tive

ro

ot g

row

th (

%)

(vs. n

o I

BA

)

** **

**

gox3-1

0

20

40

60

80

100

120

0 0.6 2 5 10

Rad

icle

em

erg

en

ce

(%

)

ABA concentration (µM)

Col-0

Col-3

bzo1-4

scpl20-1

scpl20-2

gox3-1

A

0

20

40

60

80

100

120

0 0.6 2 5 10

gapc2-2

Col-0

Rad

icle

em

erg

en

ce

(%

)

ABA concentration (μM)

gox3-1

0

20

40

60

80

100

120

Col-0 gapc2-2 pex14

** **

Supplemental Document 1. MS/MS spectra for the five known peroxisomal proteins with single peptide identification (all from Z4).

AT

3G

01980

A

T1G

77540

A

T1G

23310

A

T4G

16566

AT

1G

04710

Supplemental document 2. Amino-acid sequences for homologs of RDL1, Scpl20, UP9 and GAPC2 proteins

used in creating alignments shown in Figure 4.

>AtRDL1(At4g36880)

MAPSTKVLSLLLLYVVVSLASGDESIINDHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNNNNNGIIND

QDKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLYLGARTEPARRIAKAKNVNQKYS

AAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGINKIVTGELISLSEQELVDCDKSYN

QGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKCNSFLKNSRVVSIDGYEDVPTKDETALKKAIS

YQPVSVAIEAGGRIFQHYQSGIFTGSCGTNLDHAVVAVGYGSENGVDYWIVRNSWGPRWGEEGYIRM

ERNLAASKSGKCGIAVEASYPVKYSPNPVRGNTISSV

>BrRDL1(Bra010592)

MASSTKLLSSLLLFVVVSLASGDEFTTINNHLNLPSDGSWRTDEEVRSIYLQWCAEHGKT

SNNNGIINQQDERFNIFKDNLRFIDLHNENNKNATYKLGLTIFADLTNDEYRRLYLGART

ESVRRITKAKNVNIKYSAAVNDVEVPETVDWRQKGAVNAIKNQGTCGSCWAFSTAAAVEG

INKIVTGELISLSEQELVDCDRSYNQGCNGGLMDYAFQFIMKNGGLNTEQDYPYRGSNGK

CNSLLKNSRVVTIDGYEDVPTEDETALKRAVSYQPVSVAIEAGGRVFQHYQSGIFTGKCG

TNLDHAVVAVGYGSENGIDYWIVRNSWGTRWGEDGYIRMERNLARSKSGKCGIAVEASYP

VKYSPNPVRGTSSV

>RcRDL1(29970.m000973)

MALPISLSTLLFLFFTLSSAWDMSILSHNHGHHHQSSWRSDNEVISMYNWWLAKHSKTYN

KLGEREKRFEIFKNNLRFIDEHNNSKNRTYKVGLTRFADLTNEEYRAKFLGTKSDPKRRL

MKSKNPSQRYAFKAGDVLPESIDWRQSGAVSAIKDQGSCGSCWAFSTIAAVEGVNKIVTG

ELISLSEQELVDCDRSYNAGCNGGLMDNAFQFIINNGGIDTDKDYPYQAVDGKCDTTKVK

NKAVTIDGFEDVMAFDEMALQKAVAHQPVSVAIEASGMALQFYQSGVFTGECGSALDHGV

VIVGYGTEDGIDYWLVRNSWGRDWGENGYIKMQRNVVDTFTGKCGIAMESSYPIKNTQNP

VKISSV

>MeRDL1(cassava4.1_007020m)

MMGLFGSSAAMFVLLFLSFTLSSASDMSIISYDQTHATKSSWRTDDEVMAIYEEWLVKQG

KVYNALGEREKRFQVFKDNLRFIDEHNSENRTYKLGLNGFADLTNEEYRSTYLGARGGMK

RNRLRKTSDRYAPRVGESLPDSVDWRKEGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVT

GDLISLSEQELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYLARDGRCDTYRK

NAKVVTIDDYEDVPVNSETALQKAVANQPVSVAIEAGGRDFQFYASGIFSGRCGTQLDHG

VAAVGYGTENGKDYWIVRNSWGKSWGENGYLRMARSINSPTGICGIAMEASYPIKKGQNP

PNPGPSPPSPVTPPTVCDNYYSCPDSNTCCCLFEYGNFCFEWGCCPLEGATCCEDHYSCC

PHDYPICNINQGTCLMSKDNPLAVKAMIRIPAKPHWALGAAAKKSSA

>CsRDL1(orange1.1g017419m)

MATASMFLAISTLVFLFFISSSSAADMSIISYDNNHDHSSSWRTDDEVMTIYQTWLAKHG

KTSNGMGHNEKRFQIFKDNLRFIDEHNSLNRTYKVGLNKFADLTNEEYRAMYLGTRSDAK

RRLMKSKVASQRYACKAGDELPESVDWREKGAVNPVKDQGSCGSCWAFSTVAAVEGINKI

VTGELISLSEQELVDCDRKINAGCNGGLMDYAFQFIIQNGGMDSEQDYPYLGAENKCDPS

RRNAKVVSIDGYEDVSPFDEMSLKKAVADQPVSVAIEAGGRAFQHYESGVFTGECGSALD

HGVVAVGYGTENGVDYWLVRNSWGSDWGENGYVKLQRNLLDTNTGKCGIAMEASYPVKNS

QNSAKPKPHSSA

>ZmRDL1(GRMZM2G166281_T01)

MAAPASTTAAAAAALLLLLLLSLAAAADMSIVSYGERSDEEARRMYAEWMAAHGRTYNAV

GEEERRYQVFRDNLRYIDAHNAAADAGVHSFRLGLNRFADLTNDEYRATYLGARTRPQRE

RKLGARYHAADNEDLPESVDWRAKGAVAEVKDQGSCGSCWAFSTIAAVEGINQIVTGDLI

SLSEQELVDCDTSYNQGCNGGLMDYAFEFIINNGGIDTEKDYPYKGTDGRCDVNRKNAKV

VTIDSYEDVPANDEKSLQKAVANQPVSVAIEAAGTAFQLYSSGIFTGSCGTALDHGVTAV

GYGTENGKDYWIVKNSWGSSWGESGYVRMERNIKASSGKCGIAVEPSYPLKEGANPPNPG

PSPPSPTPAPAVCDNYYSCPDSTTCCCIYEYGKYCFAWGCCPLEGATCCDDHYSCCPHDY

PICNVRQGTCLMGKDSPLSLSVKATKRTLAKPHWAFSGNTADGMKSSA

>BrRDL1(Bradi5g24027.1)

MRSSMALVAAAALLLLVSLAAAADMSIVSYGERSEEEVRRMYVEWMAENGRTYNAIGEEERRFEVF

RDNLRYVDQHNAAADAGLHSFRLGLNRFADLTNE

EYRDTYLGVRTKPVRERRLSGRYQAADNEELPESVDWREKGAVAKVKDQGGCGSCWAFSAIAAVEG

INQIVTGDMIALSEQELVDCDTSYNQGCNGGLMD

YAFEFIINNGGIDSEEDYPYKERDNRCDANKKNAKVVTIDGYEDVPVNSELSLKKAVANQPISVAIEAG

GRAFQLYKSGIFTGRCGTALDHGVTAVGYGS

ENGKDYWIVKNSWGTVWGEDGYVRLERNIKATSGKCGIAIEPSYPLKKGANPPNPGPTPPSPAPPSTVC

DSYNECPASTTCCCIYTYGKECFAWGCCPLE

GATCCDDHYSCCPHSYPICNVQQGTCLAGKDSPMSVKALKRILAKPHGTFSGKSSA

>GmRDL1(Glyma14g09440.1)

MFTAPFPHSLSHLSPKTSPVGSSSSITTTSSKTMTMAMATILLLFTVFAVSSALDMSIIS

YDNAHAATSRSDEELMSMYEQWLVKHGKVYNALGEKEKRFQIFKDNLRFIDDHNSQEDRT

YKLGLNRFADLTNEEYRAKYLGTKIDPNRRLGKTPSNRYAPRVGDKLPESVDWRKEGAVP

PVKDQGGCGSCWAFSAIGAVEGINKIVTGELISLSEQELVDCDTGYNEGCNGGLMDYAFE

FIINNGGIDSEEDYPYRGVDGRCDTYRKNAKVVSIDDYEDVPAYDELALKKAVANQPVSV

AIEGGGREFQLYVSGVFTGRCGTALDHGVVAVGYGTANGHDYWIVRNSWGPSWGEDGYIR

LERNLANSRSGKCGIAIEPSYPLKNGPNPPNPGPSPPSPVKPPNVCDNYYSCADSATCCC

IFEFGNACFEWGCCPLEGATCCDDHYSCCPNDYPICNTYAGTCLKSKNNPFGVKALRRTP

AKPHWTFGRKNKVSSA

>PtRDL1(Potri.007G047600.1)

MALAISTLLFLFFTVSSALDMSIFNHDDNHLSHDQSSWRSDDEVMSIYKWWLQKHGKAYN

RLGEKAKRFEIFKNNLRFIDEHNSQNRTYKVGLTKFADLTNQEYRAMFLGTRSDPKRRLM

KSKNPSERYAYKAGDKLPESVDWRGKGAVNPIKDQGSCGSCWAFSTVAAVEGINQIVTGE

LISLSEQELVDCDRFYNAGCNGGLMDYAFQFIINNGGLDTEKDYPYLGNDDTCDRDKMKT

KAVSIDGFEDVLPFDEKALQKAVAHQPVSVAIEASGMALQFYQSGVFTGECGTALDHGVV

VVGYGTEKGLDYWLVRNSWGTEWGEHGYIKMQRNVRDTYTGRCGIAMESSYPVKNGQNTA

KPYLADESAGEKISSI

>PvRDL1(Phvul.001G024500.1)

MASSTSANMAIMLLFALFALSSALDMSIISYDNAHQDKATWRTDEEVNSLYEEWLVKHGK

LYNALGEKDKRFQIFKDNLRFIDQQNAENRTYKLGLNRFADLTNEEYRARYLGTKIDPNR

RLGRTPSNRYAPRVGETLPDSVDWRKEGAVVPVKDQASCGSCWAFSAIGAVEGINKIVTG

DLISLSEQELVDCDTGYNMGCNGGLMDYAFEFIIKNGGIDSEEDYPYKGVDGRCDEYRKN

AKVVSIDGYEDVNTYDELALKKAVANQPVSVAVEGGGREFQLYSSGVFTGRCGTALDHGV

VAVGYGTDNGHDFWIVRNSWGADWGEEGYIRLERNLGNSRSGKCGIAIEPSYPIKTGQNP

PNPGPSPPSPVKPPNVCDNYYSCSDSATCCCIFEFGKTCFEWGCCPLEGATCCDDHYSCC

PHDYPICNTYAGTCLRSKNNPFGVKALRRTPAKPHGAFAGNKVSNA

>SlRDL1(Solyc01g107760.2.1)

MTYFRPLFLFLLTFFVLSSALDMSIISYDEKHADLGATNHRTDDEVKGLYESWIVKHGKN

YNAIGEKEKRFEIFKDNLRFIDEQNAETRPYKLGLNRFSDLTNDEYRALFVGGRFDKKTR

LLKNPKSERYAFKAGEKLPESVDWRQKGAVAPVKDQGQCGSCWAFSTVGAVEGINQIVTG

ELISLSEQELVDCDKSYNQGCNGGLMDYAFEFIKNNGGIDTEADYPYRAKDGTCDSNRKN

ARVVSIDGYEDVPINDEKSLMKALSNQPVSVAIEAGGRAFQHYSSGVFTGYCGTQLDHGV

VAIGYGTDNGSDYWIVRNSWGPNWGESGYIRLERNLANSTSGKCGIAMEPSYPLKNGANP

PNPGPSPPSPVAPSTVCDEYYSCTAGTTCCCIYKYGDYCFGWGCCPYESATCCDDNNSCC

PHDYPVCDVNSGTCQMSKDSPLSVKALKRGPATARVNWSGMKSNRKVSYD

>AtSCPL20(At4g12910)

MSIITMVWLMKVFVFVTLLSLVFVITESAPESALITKLPGFEGTFPSKHYSGYVTIDKEHGKNLWYYFIE

SEKNPSKDPVVLWLNGGPGCSSMDGFVYEHGPFNFELPKKNNSLPLLHLNPYSWSKVSNIIYLDSPVG

VGFSYSNNKSDYITGDIKTAVDSHAFLLKWFQMFPEFQSNPFFISGESYAGVYVPTLASEVVIGNKNGV

KPALNFKGYLVGNGVADPKFDGNAFVPFAHGMGLISDELFENVTKACKGNFYEIEGLECEEQYTKVN

DDTNQLNIYNILEPCYHGTSLSAFDIRSLPSSLLQLGKTEKRLPIRKRMFGRAWPVRAPVHPGIVPSWSQ

LLADVTVPCIDDRVATAWLNDPEIRKAIHTKEESEIGRWELCSGKLSFYHDAGSMIDFHRNLTLSGYRA

LIYSGDHDMCVPFTGSEAWTKSLGYKVIDEWRAWISNDQVAGYTQGYANNLTFLTIKGAGHTVPEYK

PREALDFYSRFLEGSKI

>SbSCPL20(Sb08g006600.1)

MARGGSTAAPPPLPPLLLLLVVVVVSSAFFARCCHAAPPGALVTSVPGYTAGSGALPSKHYAGYVTVD

EAHGRRLFYYLVESERDPAKDPVVLWLNGGPG

CSSFDGFVYEHGPFNFEAGGSAGSLPKLHLNPYSWSKVSSVIYLDSPAGVGLSYSKNVSDYETGDLKT

AADSHTFLLKWFQLYPEFLKNPFYIAGESYAG

VYVPTLSHEVVKGIHKGDKPTINFKGYMVGNGVCDTVFDGNALVPFAHGMALISESIYKEANTACQG

SYWNSSSAKCDDALSKVDTALDGLNIYDILEPC

YHGTNTKEGIPQSNKLPPSFKDLGVTSKPLPVRNRMHGRAWPLRAPVRDGRVPSWQELAASVPRGVP

CMSDEVATAWLNNDNVRSAIHAEPVSSIGPWQL

CTDKIDFDHDAGSMIIYHKNLTSQGYRALIYSGDHDMCVPHTGTEAWTASLGYGIIDSWRQWIVNEQV

AGYTQGYENGLTFTTIKGSGHTVPEYKPQESL

AFYSRWLNGTKL

>OsSCPL20(LOC_Os12g15470.1)

MARRGRRSLASPAVAIALFVFLAYGGGGGGGGVCEAAPASAVVKSVPGFDGALPSKHYAGSCRVAA

AAACRYVTVEEQHGRNLFYYLVESERDPAKDPLV

LWLNGGPGCSSFDGFVYEHGPFNFESGGSAKSLPKLHLNPYSWSKVSSVIYLDSPAGVGLSYSKNTSD

YNTGDLKTAADSHTFLLKWFQLYPEFLSNPFY

IAGESYAGVYVPTLSHEVVKGLHDGVKPTINFKGYMVGNGVCDTVFDGNALVPFAHGMALISDDIYQ

EAQTACHGNYWNTTTDKCENALYKVDTSINDLN

IYDILEPCYHSKTIKKVTPANTKLPKSFQHLGTTTKPLAVRTRMHGRAWPLRAPVRAGRVPSWQEFAR

GSRPSGVPCMSDEVATAWLNNDDVRAAIHAQP

VSSIGSWLICTNVLDFIHDAGSMISYHKNLTGQGYRAFIYSGDHDMCVPYTGTEAWTRSLGYGVIDSW

RPWHLNGQVSGYTQGYEHGLTFATIKGAGHTV

PEYKPQESLAFYSRWLAGSKL

>PtSCPL20 (Potri.014G177500.1)

MAKSCPILYRIFCMLLSFVLLTHSAPETALVTQLPGFSGTFPSKHYSGYVTIDESQGKRLFYYFVESERN

PPKDPVVLWLNGGPGCSSFDGFVYEHGPFN

FEAAETKGDLPKLHLNPYSWSKVSSVLYLDSPAGVGLSYSKNETDYITGDTKTASDSHAFLLKWFELY

PEFLSNPFFISGESYAGIYVPTLAYEVVKGLD

AGVKPILNFKGYLVGNGVTDEEFDGNALVPFAHGMGLIPDELFEEVTKECTGNFYNPLGETCESKLQK

VYKDVEGLNIYDILEPCYHGSNIREVTDDRIR

LPSSFRQLGETERPLPVRKRMFGRAWPFRAPVRPGIVPTWPQLLDGESVPCTDDEVATSWLNNEAVRK

AIHAELESVSGTWELCTDRIRFHHDAGSMIKY

HRNLTLRGFRALIFSGDHDMCVPYTGSEAWTRSMGYDIVDEWRPWTSNGQVAGYTQGYANNLTFLT

MKGAGHTVPEYKPREALDFYSRFLSGKPI

>PpSCPL20 (Pp1s200_78V6.1)

MAKLSRNFGSVWVCFMIVSAVLSVGVDGLRSNLIRLATERGNGGGEDVGRKSIADPHLIVSLPGAPKF

PFSMRSGYITVDEKAGRALFFWFVEADVQDPA

SAPLTLWLNGGPGCSSVGGGMLSELGPFYPTRDGAHLLPNAHAWNKVSNMLFLESPAGVGFSYSNTT

TDYKTGDKRTAQDSYAFLLRFFEQYPLYSSSKF

YISGESYAGHYVPQLADTILEGNKVGSNKKINLQGMLVGNAWTDANVDNFGAIFFWWTHALVSDSTF

KGVVKNCNFSSVGPLRSEADDLCDKYVDIANNE

LGNINIYEIYADICVSAQAQAETRHFGKQLSRTRFGGLSTRPLMKDSYDPCVDDEVEVYLNRPEVQEAL

HANTTHLPWRWTDCSEIVDYSFDDLLSSVLP

VYHNLLESNIKILVFSGDVDAIVPVTGTRTWLNLLPLNITEAWRPWTVDNQVGGYVTKYDKLTFSTVR

GAGHMVPYTQPARALHLFQSFINNTPL

>ZmSCPL20(GRMZM2G073908_T01)

MACSPSAPLARAVTAILLLASIICCCRSAPPGALVTRLPGFDGAKLPSKHYAGYVTVDEHVGSKLFYYL

VESERDPARDPVVLWLNGGPGCSSMDGFVYE

HGPFNFESGGSSGNLPKLHLNPYSWSKVSSVIYLDSPSGVGLSYSKNVSDYETGDLKTAADSHTFLLK

WFQLYPEFQKNPFYIAGESYAGVYIPTLANQV

VQGIHKGDNPVINFKGYMVGNGVCDVTFDGNALVPFAHGMGLISDDIYEQTNTACQGNYWNYSYSE

KCADAVSNVDMVISGLNIYNILEPCYHGTNTKEV

ISRRSNNNRMPQSFKDLGVTSRPLPVRTRMFGRAWPLRAPVRDGRVPSWQELAATNNFPSVMCMNDE

VATAWLNHDSVRSAIHAEPVSAIGPWILCTDQL

LFHHDAGSMIIYHKNLTSQGYRAIIYSGDHDMCVPYTGSLAWTTSLRYGVIDSWRPWFVNGQVSGYT

QGYENGLTFATIKGSGHAVPEYKPQEALAFYSR

WLAGSKL

>BrSCPL20 (Bra032681)

MRFLLKVLAFVTLLLVFLITTESAPQSALITKLPGFSGTFPSKHYSGYVTIDKEHGKNLWYYFVESEMN

PSKDPVVLWLNGGPGCSSMDGFVYEHGPFNF

EPAKTNYSLPLLHLNPYSWSKVSNMIYLDSPVGVGFSYSKNESDYITGDMKTAVDSHAFLLKWFQMFP

EFQSNPFYISGESYAGVYVPTLASEVVKGNKE

GLKPALNLKGYLVGNGVADSMYDGNALVPFAHGMGLVSDQLFENVTKACNGNFYDNLSPDCEEQM

AKVSMDIDRLNIYNILEPCYHGTSLSAFDIKSLPS

TLLQLGKTEKPLAVRKRMFGRAWPVRAPILPGIVPSWSQLLADVSVPCIDDRVATAWLNDPAIRKAIH

AKEESEIGRWELCTGKLWFHHDAGSMISFHRN

LTLSGYRALIYSGDHDMCVPFTGSEAWTHSLGYKVIDEWRAWISNDQVAGYTEGYANNLTFLTIKAL

NHNSLLKLEKWLPRHTVPEYKPREALDFYSRFL

AGSKI

>RcSCPL20 (rco30178.m000856)

MDKPKTTSLILCCIACFLFSFVLITHSAPQDALVIEIPGFSGALPSKHYSGYVTIDESHGKKLFYYFVESE

GNPSQDPVVLWLNGGPGCSSFDGFVYEHG

PFNFEAANASADLPKLHLNPYSWSKVSNILYLDSPAGVGLSYSKNTTDYITGDIKTALDSHTFLLKWFE

LYPEFLSNPFFIAGESYAGVYVPTLAYEVMK

GIDASVKPILNLKGYLVGNGVTDELFDGNALVPFAHGMGLISDDLYEEVKDACSDNFYNPLSDTCETK

LDKVDEDIEGLNIYDILEPCYHGTDPSEVKDI

KIRLPSSFRQLGKTDRPLPVRKRMFGRAWPLRAPVRDGIVPTWPQLLNSESVPCTDDEVATLWLNNAA

VRKAIHADEESIAGTWELCTDRIFFSHDAGSM

IKYHRNLTMRGFRALIFSGDHDMCVPYTGSQAWTRSMGYKIVDEWRPWISKGQVAGYTQGYENNLT

FLTIKGAGHTVPEYKPQEAFDFYSRFLAGKRI

>GmSCPL20 (Glyma18g47820.1)

MAMENQNFLEYWVLPLLMMLLLGHWISVQAAPSPSLITQLPGFNANFPSKHYSGYISIDGNAESGKNL

FYYFVSSESSPEKDPVVLWLNGGPGCSSFDGF

VYEHGPFNFEAANSKGNLPTLHINPYSWSKVSNIIYLDSPAGVGLSYSKNTSKYATGDLETASDTHVFL

LKWFQQFPEFQANPFYIAGESYAGVYVPTLA

FEVAKGIRSGTKPVINFKGYMVGNGVTDEIFDGNALIPFVHGMGLISDSIYENLQSSCKGNYYDAYSLD

ENDVCYKTIEKVDRAIDGLNVYNILEPCYHF

PDAATAKENGTLPRSFKQLGVTERPLPVRKRMFGRAWPFRAPVKPGLVPLWPQLAQTRHVACVSDEV

ASSWLNNVAVRKAIHAESEKVAGPWELCSSRIE

YHHNAGSMIPYHKNLTRLGYRALIFSGDHDMCVPFTGSEAWTRSLGYKIVDEWRPWNSNNQVAGYL

QAYENNLTFLTIKGAGHTVPEYKPREALDFYSRW

LEGKLI

>AtUP9 (At1g29120)

MSTASSWIQQPPFRYLPDRGGFSKPSSRSARQFSSGVVSASASSPSPSSSCSCGYSEILNFDFGSNRSWNQ

QGRRVQAMSSTAQRKFSLSKGDSDDKNEPDHLLVLVHGILASPSDWLYVEAELKRRLGRRFLIYASSS

NTFTKTFGGIDGAGKRLAEEVRQVVQKSKSLKKISFLAHSLGGLFSRHAVAVLYSAAMAQVSDVAVS

QSGNSNLLRGRIAGLEPINFITLATPHLGVRGRKQLPFLLGVPILEKLAAPIAPFFVGRTGSQLFLTDGKA

DKPPLLLRMASDGEDLKFLSALGAFRSRIIYANVSYDLDMVGWRTSSIRRETELIKPSRRSLDGYKHVV

DVEYCPPVSSDGAHFPPEAAKAKEAAQSSPSPQNTLEYHEIVEEEMIRGLQRLGWKKVDVSFHSTFWP

YLAHNNIHVKSERLYKAGAGVIAHVADSIKQQESSTFITASL

>VvUP9 (GSVIVT01021209001)

MHGKYEPDHLLVLVHGILASPSDWTYAEAELKKRLGRNFLIYVSSSNTYTKTFGGIDGAGKRLADEVT

QVVQKTQSLKRISFLAHSLGGLFARYAIAVLY

TPNTSSISMIAGLEPINFITLATPHLGVRGKKQLPFLLGVPILEKIAAPIAPIFAGRTGSQLFLTDGKPNKPP

LLLRMASNCEDGKFIAALGAFSCRILY

ANVSYDHMVGWRTSSIRRETELVKPPRRSLDGYKHVVDVEYCPPVLSDGPHFPPEAAKAKEAAQNAP

STQSTVEYHEILEEEMIHGLQQLGWKKVDVSFH

SAFWPFFAHNNIHVKNEWLYNAGVGVVAHVAESLKQQESSTFIAANL

>BrUP9 (Bra010851)

MSTASWIQPPCRYYSPDRRGGGFAKPSCSRARQFPGVVSSSSCSCGYSEILNFDFGSSRSWNHQGLRVQ

AMSATAQRKFSLSKGDADEKVEPDHLLVLVH

GILASPSDWLYVEAEMKRRLGRRFLIYASSSNTFTKTFGGIDGAGKRLAEEVRQVIQKSKSLKKISFLAH

SLGGLFARHAVAVLYSAPASDGAAVSNSGN

SHLPRGLLAGLEPINFITLATPHLGVRGRKQLPFLLGVPILERLAAPLAPFVVGRTGSQLFLTDGKADKP

PLLLRMASDCEDLKFLSSLGSFRSRIVYAN

VSYDHMVGWRTSSIRRETELFKPPRRSLDGYKHVVDVEYCPPVSSDGAHFPPEAAKAKEAAQSSPSPQ

NTLEYHEIVEDEMIRGLQTLGWKKVDVSFHST

FWPYLAHNNIHVKSERLYKAGAGVVAHVADSIKQQETSTFITASL

>GmUP9 (Glyma08g12210.1)

MATAPFIHARCAYSPRISNRNNKPSSQQGPLASSSSHASSSSSPSSSSFSHPSGTNSGQRHQGLRAQAMS

TVTLGNSIASIGNLKNDPDHLLVLVHGILA

STADWTYAEAELKRRLGKNFLIYVSSSNTYTKTFTGIDGAGKRLADEVLQVVKKTKSLKRISFLAHSLG

GLFARYAIAVLYSPDTYSRDQPGDLANSMTE

NSQGTTLSRGGMIAGLEPINFITLATPHLGVRGKKQLPFLLGVPILEKLAAPIAPFFVGQTGSQLFLTDGK

PDKPPLLLRMASDSDDGKFLSALGAFRCR

IIYANVSYDHMVGWRTSSIRRETELSKKPPRQSLDGYKHVVDVEYCPPVPSDGPKFPPKAVKAKEAAQ

NAPNTQNTVEYHEIVEDEMIRGLQQLGWKKVD

VSFHSAFWPFFAHNNIHVKNEWFHNAGVGVIAHVAESLRQLEASSFLSASL

>RcUP9(Rco29900.m001618)

MATASLIQARWCGYSPIFSNNNNFRNGAFGGPASASSSHLPSSSSCSCCSSHGLNFFSGVNSNWRHQDF

RAQAMNTAIRGNFASSKGVVNGNNEPDHLLV

LVHGILASPSDWIYVEAELKKRLGRNFLIYASACNTYTKTFSGIDGAGKRLAEEVMQIVEKTDSLKKISF

LAHSLGGLFARYAIAVLYSENALSSGQSND

VADSTVSNVEAASSARRGTIAGLDPINFVTLATPHLGVRGKKQLPFLLGIPILEKLAPPIAPIFTGRTGSQ

LFLTDGKPNKPPLLLRMASDCEDGPFLSA

LGVFRCRILYANVSYDHMVGWRTSSIRREKELVKPPQRSLDGYKHVVDVEYCPPFSSDGPHFPPEAAK

AKEAAQNEPSMANTVEYHELMEEEMIRGLQQL

GWKKVDVSFHSALWPFFAHNNIHVKNEWLHNAGAGVIAHVADSLKQQECSSSPLITASL

>PtUP9 (Potri.011G065700.1)

MATASSASLFQAARYGYSPSPRFSNNCHYSVRNGGVAVGVGSSSSASSPSSSAHHGFNFSSSGVNNNW

KPQGIKSQATNTATKGRFSSSSGAMNGKSEPD

HLLVLVHGILASPSDWTYVEAELKRRLGKNFLIYASSCNTYTKTFSGIDGAGKRLADEVMRVVQKRES

LKRISFLAHSLGGLFARHAISVLYSEIAVNTG

QSIDVAADTSLPNSNTTCSSRRGMIAGLEPINFITLATPHLGVRGRKQLPFLLGIPILEKLALPIAPIIVGRT

GSQLFLTDGKPNKPSLLLRMTSDSEDG

KFLSALGAFRCRILYANVSYDHMVGWRTSSIRREMELVTPPMRSLDGYKHVVDVEYCPPVSSDGPHFP

PEAAKAKEAAQNEPSMKNTAEYHELMEEEMIS

GLQRLGWKKVDVSFHSAFWPFFAHNNIHVKNEWFHNAGTGVISHVADSLKQQESAMYIAASL

>ZmUP9 (GRMZM2G103137_T03)

MPSLRKISFLAHSLGGLFARYVIAILHSVSVETKNAGQSSALIVPTTRGPAKSRCASGLGSIAGLRPINFIT

LATPHLGVRGRNQLPFLQGLSILEKLAA

PLAPLIVGRTGAQLFLTDGDPSKAPLLLQMASDCDDKNYISALAAFKNRVLYANVSYDHMVGWRTSS

LRREKNLIKPSHRSLDGYKHIVNVEYCSPVSSE

GPHFPSKAARAKEAAQRSPNRENTEEYHQMMEEEMLHGLQKVGWKKVDVNFHSSFWPYSAHNNIHV

KNEWLHNAGAGVIAHVADSIKQQESRPCLPANL

>SmUP9 (smo101352)

MKSCAIGSASTSKETNQARECPDHLLVLVHGILSSPSDWKYFKQELKARLGSKFYIHASSVNSYLQTLT

GIDMAGKRLADEVSEVVQKHPQLQRISFVAH

SLGGLFARYAIGILYLPLQPSKKLMNPSITGLDPVSFITLATPHLGVRGRQQLPFLMGVPVLEKIAAPIAP

FIVGRTGRQLFLTDGKASDPPLLLRMATD

CEQGQFISALRAFKLRAVYANVSYDHMVGWRTSSIRRESELSKPPRKSLEGYKHVVNVRYCPAIQSDG

PSFEDNTAGAKEAAQKQPSSIEAKEYHDILEE

EMVRGLQQVSWQKVDVSFHSAIWPFFAHNNIHVKQEWLHYEGAGVVSHVADTLKQQEHGAFLEANL

>PpUP9 (Pp1s59_53V6.1)

MKGMSSRNISEHLPAMQPRSKKGQQCDDIQPEHLLVLVHGILSSPADWEYVQKALQRRLGNKFLIHAS

AVNSFLNTLGGIDHAGRRLASEIERIVEKVPS

LKRISFLAHSLGGLFARYAVAMLYTSKDDITEDMSTLEDFESRGEEHPVLRLRREPKIAGLEAVNYITLA

SPHLGVRGKKQLPFLLGVQVLEKLAAPIAP

FVVGRTGRQLFLTDGKASDPPLLLRMASDCSEGLFISALRAFKSRVVYANVSYDHMVGWRTSSIRRES

ELSKPPRVSMDGYKHVVNVAYYPAVNSDAPSF

QQESAQGKAAAQASPSSKKADAYHDTLEEEMVRGLQQVSWRKVDVSFHSALWPFLAHNSITVKDRW

IHYEGTGVIAHVVDAFAQQEQNVFLVASL

>AtGAPC2 (At1g13440)

MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKHHELKVKD

DKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVVESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMF

VVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHSITATQKTVDGPSMKDWRGG

RAASFNIIPSSTGAAKAVGKVLPSLNGKLTGMSFRVPTVDVSVVDLTVRLEKAATYDEIKKAIKEESEG

KMKGILGYTEDDVVSTDFVGDNRSSIFDAKAGIALSDKFVKLVSWYDNEWGYSSRVVDLIVHMSKA

>ZmGAPC2(GRMZM2G071630_T01)

MAKIKIGINGFGRIGRLVARVALQSDDVELVAVNDPFISTDYMTYMFKYDTVHGQWKHHEVKVKDSK

TLLFGEKEVAVFGCRNPEEIPWGSVGAEYVVES

TGVFTDQEKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEKEYKSDINIVSNASCTTNCLAPLAKVIND

KFGIVEGLMTTVHAITATQKTVDGPSSKDWRG

GRAASFNIIPSSTGAAKAVGKVLPVLNGKLTGMSFRVPTVDVSVVDLTVRLEKSATYDEIKAAVKAEA

EGSLKGILGYVEEDLVSTDFQGDSRSSIFDAK

AGIALNGNFVKLVSWYDNEWGYSTRVVDLIRHMNSTK

>MdGAPC2(MDP0000645828)

MDKIRQTECIRKCSDKVYASVAYGVPRPLGDRGNMHVKGLEGHRAKNLLQPKARPEGSWAPQNLKG

PKGQKVEPIQPTSQLGAGQKFKKSCRFGRIGRLV

ARVALQRDDVELVAVNDPFITTDYMTYMFKYDTVHGAWKHHELKVKDSKTLLFGEKPVAVFGIRNP

EEIPWGEAGADFVVESTGVFTDKEKAAAHIKGGA

KKVIISAPSKDAPMFVVGVNEKEYKPDIHILSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHSITATQK

TVDGPSMKDWRGGRAASFNIIPSSTGAAKA

VGKVLPSLNGKLTGMSFRVPTVDVSVVDLTVRLEKAATYEQIKAAIKEESEGKLKGILGYTEDDVVST

DFIGDSRSSIFDAKAGIALNDNFVKLVSWYDN

EWGYSSRVVDLIVHVASSL

>MeGAPC2(cassava4.1_011366m)

MGKVKIGINGFGRIGRLVARVALQRDDVELVAVNDPFITVEYMTYMFKYDTVHGQWKHHELKVKDD

KTLLFGEKPVTVFGVRNPEEIPWAETGAEYIVES

TGVFTDKDKAAAHLKGGAKKVIISAPSKDAPMFVVGVNEKEYKPDLHIVSNASCTTNCLAPLAKVIHD

RFGIVEGLMTTVHSITATQKTVDGPSMKDWRG

GRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTVDVSVVDLTVRLEKKATYDQIKAAIKEES

EGKLKGILGYTEDDVVSTDFIGDNRSSIFDAK

AGIALNDNFVKLVAWYDNEWGYSTRVVDLIRHIASIQ

>RcGAPC2(RCO30147.m013783)

MAKIKIGINGFGRIGRLVARVALQRDDVELVAVNDPFISTEYMTYMFKYDTVHGQWKHHELKIKDDK

TLLFGEKPVTVFGVRNPEEIPWAETGAEYIVES

TGVFTDKDKAAAHLKGGAKKVIISAPSKDAPMFVVGVNEKEYTPDLHIISNASCTTNCLAPLAKVIND

RFGIVEGLMTTVHSITATQKTVDGPSMKDWRG

GRAASFNIIPSSTGAAKAVGKVLPSLNGKLTGMSFRVPTVDVSVVDLTVRLEKSATYEQIKAAIKEESE

GKLKGILGYTEDDVVSTDFIGDKRSSIFDAK

AGIALNDKFVKLVSWYDNEWGYSTRVVDLIVHVASVH

>GmGAPC2(Glyma18g01330.1)

MASDKKIRIGINGFGRIGRLVARVALQRNDVELVAVNDPFITTDYMTYMFKYDTVHGQWKHFDVKVK

DSKTLLFGEKPVTVFGIRNPEEIPWGEVGADYV

VESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEKEYKPELDIVSNASCTTNCLAPLAK

VINDRFGIVEGLMTTVHAITATQKTVDGPSNKD

WRGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMSFRVPTVDVSVVDLTVRLEKPATYEQIKSAIK

EESEGKLKGILGYTEDDVVSTDFVGDNRSSIF

DAKAGISLNDNFVKLVSWYDNEWGYSSRVIDLIVHIASVA

>PtGAPC2(Potri.001G335800.1)

MAKIKIGINGFGRIGRLVARVALQRDDVELVAVNDPFITTDYMTYMFKYDTVHGQWKHGDIKVKDEK

TLLFGDKPVAVFGVRNPEEIPWGAAGADFVVES

TGVFTDKDKAAAHLKGGAKKVIISAPSKDAPMFVMGVNEKEYKPDLNIVSNASCTTNCLAPLAKVIH

DKFGIVEGLMTTVHSITATQKTVDGPSMKDWRG

GRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTVDVSVVDLTVRIEKKATYDQIKAAIKEESE

TNLKGILGYTEDDVVSTDFMGDSRSSIFDAK

AGIALNDNFVKLVSWYDNEWGYSSRVIDLIIHIASVQ

>MtGAPC2(Medtr4g103920.1)

MGGAKIKIGINGFGRIGRLVARVALKRDDVELVAVNDPFITTDYMTYMFKYDSVHGQWKNDELTVKD

SKTLLFGEKPVTVFAHRNPEEIPWASTGADIIV

ESTGVFTDKDKAAAHLKGGAKKVIISAPSKDAPMFVVGVNEKEYKPEFDIISNASCTTNCLAPLAKVIN

DRFGIVEGLMTTVHSITATQKTVDGPSAKDW

RGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTVDVSVVDLTVRLEKPATYDQIKAAIKE

ESEGNLKGILGYTEDDVVSTDFIGDTRSSIFD

AKAGIALNDNFVKLVSWYDNELGYSTRVVDLIVHIAKQL

>BrGAPC2 (Bra026904)

MADKKIKIGINGFGRIGRLVARVILQRNDVELVAVNDPFITTEYMTYMFKYDSVHGQWKHNELKLKD

EKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVV

ESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNGHEYKSDLDIVSNASCTTNCLAPLAKV

INDRFGIVEGLMTTVHSITATQKTVDGPSMKDW

RGGRAASFNIIPSSTGAAKAVGKVLPQLNGKLTGMSFRVPTVDVSVVDLTVRLEKAATYDDIKKAIKE

ESEGKLKGILGYTEDDVVSTDFVGDSRSSIFD

AKAGIALSDNFVKLVSWYDNEWGYSTRVVDLIIHMSKA

>SlGAPC2(Solyc05g014470.2.1)

MANGKIKIGINGFGRIGRLVARVALQRDDVELVAVNDPFISTDYMTYMFKYDSVHGQWKHHELKVK

DEKTLLFGEKAVTVFGIRNPEDIPWGEAGADFVV

ESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNENEYKPELDIVSNASCTTNCLAPLAKVI

NDRFGIVEGLMTTVHAMTATQKTVDGPSMKDW

RGGRAASFNIIPSSTGAAKAVGKVLPQLNGKLTGMAFRVPTADVSVVDLTVRLEKEASYEDIKAAIKE

ESEGKLKGILGYTEDDVVSTDFVGDSRSSIFD

AKAGIALSKNFVKVVSWYDNEWGYSSRVIDLICHMAKA

> UM10167[Ustilago maydis glyceraldehyde-3-phosphate dehydrogenase]

MSQVNIGINGFGRIGRIVFRNSVVHNTANVVAINDPFIDLEYMVYMLKYDSTHGVFNGDI

STKDGKLIVNGKSIAVFAEKDPSNIPWGQAGAHYVVESTGVFTTIDKASAHIKGGAKKVV

ISAPSADAPMYVCGVNLDAYDPKAQVVSNASCTTNCLAPLAKVIHDKFGIVEGLMTTVHA

TTATQKTVDGPSAKDWRGGRAAAANIIPSSTGAAKAVGKVIPSLNGKLTGMAFRVPTTNV

SVVDLTARLEKGASYDEIKAEVKRASENELKGILGYTEDAVVSQDFIGNSHSSIFDAAAG

ISLNNNFVKLVSWYDNEWGYSNRCLDLLVFMAQKDSAGASRL

>gi|7669492|ref|NP_002037.2| glyceraldehyde-3-phosphate dehydrogenase isoform 1 [Homo sapiens]

MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGK

LVIN

GNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHE

KY

DNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPAS

TGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYT

EHQ

VVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

>gi|74212012|dbj|BAE40174.1| unnamed protein product [Mus musculus]

MVEVGVNGFGRIGRLVTRAAICSGKVEIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVIN

GK

PITIFQERDPTNIEWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKY

DN

SLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPAST

G

AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTED

QVV

SCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE

Supplemental Table IX. Proteins tested for subcellular localization in this study.

PTS1/2, peroxisome targeting signal type 1 or 2. Unverified PTSs are enclosed in parentheses. YFP orientation indicates where YFP

was located relative to the coding sequence of the gene, here NY and CY refer to YFP-gene and gene-YFP fusions respectively. T, Z,

M, E and R refer to Total, Zoom, Membrane fraction proteomics experiments in this study, and studies by Eubel et al. 2008 and

Reumann et al. 2009, respectively. Proteins that were found are indicated by x, and c in case they were reported as contaminant.

Gene model Acronym Annotation PTS1/2 YFP

orientation

Localization result T Z M E R

At1g13440.1 GAPC2 Glyceraldehyde 3-phosphate dehydrogenase C2 (SKA>) NY

cytosol &

peroxisome x x x c c

At1g14810.1 Semialdehyde dehydrogenase family protein (MLL>) NY cytosol x

At1g26340.1 B5#6 Cytochrome B5 isoform A - NY cytosol x x

CY cytosol x x

At1g29090.1 Peptidase C1A papain family protein - NY cytosol x x x

CY cytosol x x x

At1g29120.5 UP9 Unknown protein ASL> NY peroxisome x

At1g32060.1 PRK Phosphoribulokinase (AKA>) NY cytosol x

At1g47128.1 RD21 Response to dehydration 21/Cysteine-type peptidase - CY cytosol x

At1g65880.1 BZO1 Benzoate-CoA ligase 1 SRL> NY peroxisome x

At1g71250.1 GDSL-motif lipase/hydrolase family protein - CY cytosol x x

At1g78380.1 GSTU19 Glutathione S-transferase tau 19 - NY mitochondria x x

CY mitochondria x x

At2g16600.1 ROC3 Peptidyl-prolyl cis-trans isomerase - CY cytosol x x

At2g21660.1 CCR2 Cold, circadian rhythm, and RNA binding 2 - NY nucleus x x x x

CY nucleus x x x x

At2g28490.1 Cupin family protein - CY cytosol x x

At2g30970.1 ASP1 Aspartate aminotransferase 1 - CY cytosol x

At2g31670.1 UP3 Unknown protein SSL> NY peroxisome x x x

At2g36640.1 ATECP63 Embryonic cell protein 63 (GKL>) NY cytosol x

At2g38380.1 PER22 Peroxidase 22 SSI> CY cytosol x x

At2g38530.1 LTP2 Lipid transfer protein 2 - CY cytosol x x

At2g42520.1 RH37 DEAD box RNA helicase - NY non-peroxisomal dots x x

CY non-peroxisomal dots x x

At2g47730.1 GSTF8 Glutathione S-transferase phi8 - CY chloroplast x x c

At3g11630.1 2-cys peroxiredoxin (RVxSL) CY non-peroxisomal dots x x c

At3g48170.1 BADH Aldehyde dehydrogenase SKL> NY peroxisome x x x

At3g48730.1 Glutamate-1-semialdehyde 2,1-aminomutase 2 SRL> NY cytosol x

At3g55290.1 SDRd Short-chain dehydrogenase/ reductase SSL> NY peroxisome x x x x

CY cytosol x x x x

At4g12910.1 Scpl20 Serine carboxypeptidase-like 20 SKI> NY peroxisome x x

At4g18360.1 GOX3 Glycolate oxidase isoform 3 AKL> NY peroxisome x

At4g20360.1 RabE1b/8D Rab GTPase homolog E1b - NY non-peroxisomal dots x x c

CY non-peroxisomal dots x x c

At4g34200.1 EDA9 Embryo sac development arrest 9 LKL> NY cytosol x x x

At4g36880.1 RDL1 Papain-like cysteine proteinase SSV> NY peroxisome x x

At4g39260.1 CCR1 RNA binding / nucleic acid binding - NY nucleus x x x x

CY nucleus x x x x

At5g16370.1 AAE5 Acyl-activating enzyme 5 SRM> NY peroxisome x x x x

At5g17920.1 ATMS1 Cobalamin-independent methionine synthase (SAK>) NY peroxisome x x x

At5g19550.1 AAT2 Aspartate aminotransferase 2 - CY non-peroxiosmal dots x x c

At5g44020.1 Acid phosphatase class B family protein (RSxTL) CY cytosol x x

At5g46290.1 KAS1 3-Ketoacyl-acyl carrier protein synthase I - NY dots x

CY dots x

At5g48230.2 ACAT2 Acetoacetyl-CoA thiolase 2 (ELL >) NY cytosol x x x

CY cytosol x x x

Supplemental Table X. Comparison of peroxisomal proteins identified from three proteomics studies.

Tissue/Material Cell culture Leaf Etiolated seedlings

Species Arabidopsis Arabidopsis Arabidopsis

Reference Eubel et al., 2008

Reumann et al., 2009 This study

AGI no Gene Name Annotation

At1g01710 ACH2 Acyl-CoA thioesterase X X X

At1g01820 PEX11c Peroxin 11 c X X

At1g04290 sT4 Small thioesterase 4 X X

At1g04710 KAT1 3-Ketoacyl-CoA thiolase 1 X X X

At1g06290 ACX3 Acyl-CoA oxidase 3 X X X

At1g06460 ACD31.2 Small heat shock protein X

At1g11840 GLX1 Glyoxylase I homolog X

At1g13440 GAPC2 Glyceraldehyde 3-phosphate dehydrogenase C2 * * X

At1g16730 UP6 Unknown protein 6 X X

At1g19570 DHAR Dehydroascorbate reductase 1 X

At1g20480 4CLP3 4-coumarate:CoA ligase 3 X

At1g20510 OPCL1 OPC-8:0 ligase 1 X X

At1g20560 AAE1 Acyl-activating enzyme 1 X X

At1g20620 CAT3 Catalase 3 X X X


At1g21770 ATF1 Acetyl transferase 1 X

At1g23310 GGT1 Glutamate-glyoxylate aminotransferase 1 X X X

At1g28320 DEG15 Deg/HtrA protease X

At1g29120 UP9 Unknown protein 9 X

At1g29260 PEX7 Peroxin 7 X

At1g47750 PEX11a Peroxin 11a X X X

At1g48320 st1 Small thioesterase 1 X X

At1g49350 pxPfkB PfkB-type carbohydrate kinase family protein X

At1g49670 NQR NADH:quinone reductase X X X

At1g50510 IndA Indigoidine synthase A X X X

At1g54340 ICDH NADP-dependent isocitrate dehydrogenase X X X

At1g60550 NS Naphthoate synthase X X

At1g64850 CEF Calcium binding EF-hand X

At1g65520 ECI monofunctional enoyl CoA hydratase/isomerase c X X

At1g65840 PAO4 Polyamine oxidase 4 X

At1g65880 BZO1 BENZOYLOXYGLUCOSINOLATE 1 X

At1g68010 HPR1 Hydroxypyruvate reductase 1 X X X

At1g70580 GGT2 Glutamate-glyoxylate aminotransferase 2 X X X

At1g76150 ECH2 Monofunctional enoyl-CoA hydratase 2 X X X

At1g77540 ATF2 Acetyltransferase X X

At1g78370 GSTU20 Glutathione S-Transferase tau 20 X

At2g06050 OPR3 12-oxophytodienoate reductase 3 X X X

At2g13360 SGAT1 Serine-glyoxylate aminotransferase X X

At2g22780 PMDH1 Peroxisomal NAD+-malate dehydrogenase 1 X X X

At2g26230 Uri Uricase X X X

At2g27490 CoAE Dephospho-CoA kinase X

At2g29590 st5 Small thioesterase 5 X

At2g31670 UP3 Unknown protein X X

At2g33150 KAT2 3-Ketoacyl-CoA thiolase 2 X X X

At2g35690 ACX5 Acyl-CoA oxidase 5 X X

At2g38180 GLH GDSL motif lipase/hydrolase family protein X

At2g39970 PXN/PMP38/PMP36 Peroxisomal membrane protein of 36 Kda X X X

At2g41790 PXM16 PXM16 Peptidase M16 family X X

At2g42490 CuAO Copper amine oxidase X X X

At2g42790 CSY3 Citrate synthase 3 X X X

At2g45740 PEX11d Peroxin 11d X X X

At3g01910 SO Sulfite oxidase X X X

At3g01980 SDRc Short-chain dehydrogenase/reductase c X X

At3g02360 6PGDH Phosphogluconate dehydrogenase X X X

At3g05290 PNC1 Peroxisomal adenine nucleotide carrier 1

At3g05970 LACS6 Long-chain acyl-CoA synthetase 6 X X X

At3g06810 IBR3 IBA-response 3 X X X

At3g06860 MFP2 Fatty acid multifunctional protein 2 X X X

At3g12800 SDRb Short-chain dehydrogenase/ reductase b X X X

At3g14150 HAOX1 Hydroxy-acid oxidase 1 X

At3g14415 GOX1 Glycolate oxidase 1 X X X


At3g15290 HBCDH Hydroxybutyryl-CoA dehydrogenase X X X


At3g17420 GPK1 Glyoxysomal protein kinase 1

At3g21720 ICL Isocitrate lyase X

At3g24170 GR1 Glutathione reductase 1 X X

At3g27820 MDAR4 Monodehydroascorbate reductase 4 X

At3g47430 PEX11b Peroxin 11b X

At3g48140 B12D1 Senescence-associated protein/ B12D-related protein X

At3g48170 BADH Aldehyde dehydrogenase X X X

At3g51660 MIF Macrophage migration factor X


At3g52880 MDAR1 Monodehydroascorbate reductase 1 X X

At3g55290 SDR Short-chain dehydrogenase/ reductase X X X

At3g55640 CDC Ca2+

dependent carrier X X

At3g56460 ZnDH Zinc-binding dehydrogenase X X X

At3g56490 HIT3 Histidine triad family protein 3 X

At3g58750 CSY2 Citrate synthase 2 X X X

At3g61070 PEX11e Peroxin 11e X X X

At3g61200 st3 Small thioesterase 3 X X X

At4g00520 ACH Acyl-CoA thioesterase family protein X

At4g02340 EH3 Epoxide hydrolase 3 X X

At4g04320 MCD Malonyl-CoA decarboxylase X X

At4g04470 PMP22 Peroxisomal membrane protein of 22 kDa X

At4g05160 4CLP1 4-coumarate:CoA ligase 1 X X X

At4g05530 IBR1 Indole-3-butyric acid response 1 X X X

At4g09320 NDPK1 Nucleoside diphosphate kinase type 1 X X

At4g12910 SCPL20 Serine carboxypeptidase-like 20 X

At4g14430 IBR10 Indole-3-butyric acid response 10 X X X

At4g16210 ECHIA Monofunctional enoyl-CoA hydratase/ isomerase a X X X

At4g16566 HIT1 Histidine triad family protein 1 X X X



At4g29010 AIM1 Abnormal inflorescence meristem 1 X X X

At4g35000 APX3 Ascorbate peroxidase 3 X X X


At4g36880 RDL1 Cysteine proteinase X

At4g39850 PXA1/CTS Peroxisomal ABC transporter 1/ Comatose X X X

At5g03860 MLS Malate synthase X

At5g09660 PMDH2 Peroxisomal NAD-malate dehydrogenase 2 X X X

At5g11520 ASP3 Aspartate aminotransferase X X X

At5g11910 ELT1 Esterase/lipase/thioesterase family 1 X

At5g16370 AAE5 Acyl-activating enzyme 5 X X X

At5g17920 ATMS1 Cobalamin-independent methionine synthase X X

At5g18100 CSD3 Copper/zinc superoxide dismutase 3 X X


At5g24400 6PGL 6-Phosphogluconolactonase

At5g27520 PNC2 Peroxisomal adenine nucleotide carrier 2 X

At5g27600 LACS7 Long-chain acyl-CoA synthetase 7 X X X

At5g37670 AtHsp15.7 Heat shock protein similar to 17.6 kDa class I X

At5g41210 GSTT1 Glutathione S-transferase θ isoform 1 X X X

At5g42890 SCP2 Sterol carrier protein 2 X X X

At5g43280 AtDCI Δ3,5- Δ2,4-enoyl-CoA-isomerase X X X


At5g47040 LON2 Lon protease homolog 2 * X X

At5g47720 ACAT1.3 Acetoacetyl-CoA thiolase 1.3 X

At5g48545 HIT2 Histidine triad family protein 2 X

At5g48880 KAT5 3-Ketoacyl-CoA thiolase 5 X

At5g58220 TLP Transthyretin-like protein X X

At5g62810 PEX14 Peroxin 14 X X X

At5g63380 4CLP2 4-coumarate:CoA ligase 2 X

At5g65110 ACX2 Acyl-CoA oxidase 2 X X


At5g65940 CHY1 3-hydroxyisobutyryl-CoA hydrolase X X X

82 104 77

* Detected but not claimed to be peroxisomal 2 1

Total 84 105 77

Supplemental Table XI. List of proteins common or exclusive to the peroxisomal proteome of leaves, cell cultures and etiolated seedlings.

AGI No. Name Annotation

Exclusively in etiolated seedling peroxisome

1 At1g29120 UP9 Unknown protein 9

2 At1g65880 BZO1 Benzoyloxyglucosinolate 1

3 At3g21720 ICL Isocitrate lyase

4 At4g12910 SCPL20 Serine carboxypeptidase-like 20

5 At4g36880 RDL1 Cysteine proteinase

6 At5g03860 MLS Malate synthase

7 At5g37670 AtHsp15.7 Heat shock protein similar to 17.6 kDa class I

8 At5g47720 ACAT1.3 Acetoacetyl-CoA thiolase 1.3

Exclusively in cell culture peroxisome

1 At1g20480 4CLP3 4-coumarate:CoA ligase 3

2 At1g28320 DEG15 Deg/HtrA protease

3 At1g29260 PEX7 Peroxin 7

4 At1g49350 pxPfkB PfkB-type carbohydrate kinase family protein

5 At1g65840 PAO4 Polyamine oxidase 4

6 At2g38180 GLH GDSL motif lipase/hydrolase family protein

7 At4g00520 ACH Acyl-CoA thioesterase family protein

8 At4g04470 PMP22 Peroxisomal membrane protein of 22 kDa

9 At5g27520 PNC2 Peroxisomal adenine nucleotide carrier 2

Exclusively in leaf peroxisome

1 At1g06460 ACD31.2 Small heat shock protein

2 At1g11840 GLX1 Glyoxylase I homolog

3 At1g19570 DHAR Dehydroascorbate reductase 1

4 At1g21770 ATF1 Acetyl transferase 1

5 At1g64850 CEF Calcium binding EF-hand

6 At1g78370 GSTU20 Glutathione S-Transferase tau 20

7 At2g27490 CoAE Dephospho-CoA kinase

8 At2g29590 sT5 Small thioesterase 5

9 At3g14150 HAOX1 Hydroxy-acid oxidase 1

10 At3g27820 MDAR4 Monodehydroascorbate reductase 4

11 At3g47430 PEX11b Peroxin 11b

12 At3g48140 B12D1 Senescence-associated protein/ B12D-related protein

13 At3g51660 MIF Macrophage migration factor

14 At3g56490 HIT3 Histidine triad family protein 3

15 At5g11910 ELT1 Esterase/lipase/thioesterase family 1



18 At5g48880 KAT5 3-Ketoacyl-CoA thiolase 5



Common to etiolated seedling peroxisomes, cell culture peroxisomes and leaf peroxisome


2 At1g06290 ACX3 Acyl-CoA oxidase 3

3 At1g13440 GAPC2 Glyceraldehyde 3-phosphate dehydrogenase C2

4 At1g20620 CAT3 Catalase 3

5 At1g23310 GGT1 Glutamate-glyoxylate aminotransferase 1

6 At1g47750 PEX11a Peroxin 11a


8 At1g49670 NQR NADH:quinone reductase

9 At1g50510 IndA Indigoidine synthase A

10 At1g54340 ICDH NADP-dependent isocitrate dehydrogenase

11 At1g68010 HPR1 Hydroxypyruvate reductase 1

12 At1g70580 GGT2 Glutamate-glyoxylate aminotransferase 2

13 At1g76150 ECH2 Monofunctional enoyl-CoA hydratase 2

14 At2g06050 OPR3 12-oxophytodienoate reductase 3

15 At2g22780 PMDH1 Peroxisomal NAD+-malate dehydrogenase 1

16 At2g26230 Uri Uricase


18 At2g39970 PXN Peroxisomal membrane protein of 36 Kda

19 At2g42490 CuAO Copper amine oxidase

20 At2g42790 CSY3 Citrate synthase 3

21 At2g45740 PEX11d Peroxin 11d

22 At3g01910 SO Sulfite oxidase

23 At3g02360 6PGDH Phosphogluconate dehydrogenase

24 At3g05970 LACS6 Long-chain acyl-CoA synthetase 6

25 At3g06810 IBR3 IBA-response 3

26 At3g06860 MFP2 Fatty acid multifunctional protein 2

27 At3g12800 SDRb Short-chain dehydrogenase/ reductase b

28 At3g14415 GOX1 Glycolate oxidase 1


30 At3g15290 HBCDH Hydroxybutyryl-CoA dehydrogenase

31 At3g48170 BADH Aldehyde dehydrogenase


33 At3g55290 SDRd Short-chain dehydrogenase/ reductase

34 At3g56460 ZnDH Zinc-binding dehydrogenase

35 At3g58750 CSY2 Citrate synthase 2

36 At3g61070 PEX11e Peroxin 11e



39 At4g05530 IBR1 Indole-3-butyric acid response 1

40 At4g14430 IBR10 Indole-3-butyric acid response 10

41 At4g16210 ECHIA Monofunctional enoyl-CoA hydratase/ isomerase a




45 At4g29010 AIM1 Abnormal inflorescence meristem 1

46 At4g35000 APX3 Ascorbate peroxidase 3

47 At4g35090 CAT2 Catalase 2

48 At4g39850 PXA1 Peroxisomal ABC transporter 1/ Comatose

49 At5g09660 PMDH2 Peroxisomal NAD-malate dehydrogenase 2

50 At5g11520 ASP3 Aspartate aminotransferase

51 At5g16370 AAE5 Acyl-activating enzyme 5

52 At5g27600 LACS7 Long-chain acyl-CoA synthetase 7

53 At5g41210 GsTT1 Glutathione S-transferase θ isoform 1

54 At5g42890 SCP2 Sterol carrier protein 2

55 At5g43280 AtDCI Δ3,5- Δ2,4-enoyl-CoA-isomerase

56 At5g47040 LON2 Lon protease homolog 2


58 At5g65940 CHY1 3-hydroxyisobutyryl-CoA hydrolase

Common to cell culture peroxisomes & leaf peroxisomes missing in etiolated seedling peroxisome

1 At1g01820 PEX11c Peroxin 11 c


3 At1g20510 OPCL1 OPC-8:0 ligase 1


5 At1g65520 ECI monofunctional enoyl CoA hydratase/isomerase c

6 At2g13360 SGAT1 Serine-glyoxylate aminotransferase


8 At2g41790 PXM16 PXM16 Peptidase M16 family


10 At3g24170 GR1 Glutathione reductase 1

11 At3g55640 CDC Ca2+ dependent carrier


13 At4g02340 EH3 Epoxide hydrolase 3

14 At4g04320 MCD Malonyl-CoA decarboxylase


16 At5g58220 TLP Transthyretin-like protein

Common to leaf peroxisomes and etiolated seedling peroxisomes but not in cell culture peroxisome



3 At1g60550 NS Naphthoate synthase

4 At1g77540 ATF2 Acetyltransferase

5 At2g31670 UP3 Unknown protein

6 At3g01980 SDRc Short-chain dehydrogenase/reductase c

7 At4g09320 NDPK1 Nucleoside diphosphate kinase type 1

8 At5g17920 ATMS1 Cobalamin-independent methionine synthase

9 At5g18100 CSD3 Copper/zinc superoxide dismutase 3


Present in leaf peroxisomes not in etiolated seedling peroxisome



4 At1g06460 ACD31.2 Small heat shock protein

5 At1g11840 GLX1 Glyoxylase I homolog

6 At1g19570 DHAR Dehydroascorbate reductase 1



9 At1g21770 ATF1 Acetyl transferase 1

10 At1g64850 CEF Calcium binding EF-hand


13 At1g78370 GSTU20 Glutathione S-Transferase tau 20


15 At2g27490 CoAE Dephospho-CoA kinase




20 At3g14150 HAOX1 Hydroxy-acid oxidase 1




24 At3g47430 PEX11b Peroxin 11b

25 At3g48140 B12D1 Senescence-associated protein/ B12D-related protein

26 At3g51660 MIF Macrophage migration factor






32 At5g11910 ELT1 Esterase/lipase/thioesterase family 1






38 At5g63380 4CL2 4-coumarate:CoA ligase 2


Present in cell culture peroxisomes not in etiolated seedling peroxisome






6 At1g28320 DEG15 Deg/HtrA protease


8 At1g49350 pxPfkB PfkB-type carbohydrate kinase family protein


10 At1g65840 PAO4 Polyamine oxidase 4



13 At2g38180 GLH GDSL motif lipase/hydrolase family protein






19 At4g00520 ACH Acyl-CoA thioesterase family protein



22 At4g04470 PMP22 Peroxisomal membrane protein of 22 kDa


24 At5g27520 PNC2 Peroxisomal adenine nucleotide carrier 2


Supplemental Table XV. Primers used for subcellular localization.

Gene model Primer Sequence 5'-3' Direction Orientation Comments

At1g13440.1

HU5522 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCTGACAAGAAGAT For PTS1

HU5523 GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAGGCCTTTGACATGTG Rev PTS1

At1g14810.1

HU3237 AAAAAGCAGGCTTCGCGACGTTCACTCATCAAACC For PTS1 AdaptorF

HU3238 AGAAAGCTGGGTTCAGAGAAGCATCTCAGCGATCTG Rev PTS1 AdaptorR

At1g26340.1

HU3569 AAAAAGCAGGCTTCCCGACACTCACAAAGCTTTATTC For PTS1 AdaptorF

HU3570 AGAAAGCTGGGTTTAAGTCTTGCGAGAGAACAAG Rev PTS1 AdaptorR

HU3571 AAAAAGCAGGCTATGCCGACACTCACAAAGCTTTATTC For PTS2 AdaptorF

HU3572 AGAAAGCTGGGTGAGTCTTGCGAGAGAACAAGAC Rev PTS2 AdaptorR

At1g29090.1

HU3432 AAAAAGCAGGCTATGTGTGATTCTTTCATCAATAAC For PTS1 AdaptorF

HU3433 AGAAAGCTGGGTGGGCAACCGGATAAAAAGCATAC Rev PTS1 AdaptorR

HU3776 GAGAATGCCCAACAACATCCACATTGGCCTTGGTATTTAAC For PTS2 AdaptorF

HU3777 GTTAAATACCAAGGCCAATGTGGATGTTGTTGGGCATTCTC Rev PTS2 AdaptorR

At1g29120.5

HU3151 AAAAAGCAGGCTTCTCTACGGCTTCTTCTTGGATTC For PTS1 AdaptorF

HU3152 AGAAAGCTGGGTTTATAAGCTGGCGGTAATAAAC Rev PTS1 AdaptorR

At1g32060.1

HU3270 AAAAAGCAGGCTTCGCTGTCTCAACTATCTACTC For PTS1 AdaptorF

HU3271 AGAAAGCTGGGTTTAGGCTTTAGCTTCTGCACG Rev PTS1 AdaptorR

At1g47128.1

HU3434 AAAAAGCAGGCTATGGGGTTCCTTAAGCCAAC For PTS2 AdaptorF

HU3435 AGAAAGCTGGGTGGGCAATGTTCTTTCTGCCTTG Rev PTS2 AdaptorR

At1g65880.1

HU3272 AAAAAGCAGGCTTCGATGATTTGGCATTATGTGAAG For PTS1 AdaptorF

HU3273 AGAAAGCTGGGTTCAAAGCCGCGAAATAAAATGTC Rev PTS1 AdaptorR

At1g71250.1

HU3428 AAAAAGCAGGCTATGAACACAAACAGAAAGAAG For PTS2 AdaptorF

HU3429 AGAAAGCTGGGTGGTGAAGGAGTGTCATCTGTTGG Rev PTS2 AdaptorR

At1g78380.1

HU2203 GGGGACAAGTTTGTACAAAAAAGCAGGCTATGGCGAACGAGGTGATTCT For PTS1

HU2204 GGGGACCACTTTGTACAAGAAAGCTGGGTCTTACTCAGGTACAAATTTCT Rev PTS1

HU2205 GGGGACCACTTTGTACAAGAAAGCTGGGTCCTCAGGTACAAATTTCT Rev PTS2 +HU2203

At2g16600.1

HU2378 AAAAAGCAGGCTATGGCAACAAACCCTAAAGTCTAC For PTS2 AdaptorF

HU2379 AGAAAGCTGGGTGAGAAATCTGACCACAATCAGCAATG Rev PTS2 AdaptorR

At2g21660.1

HU2646 AAAAAGCAGGCTATGGCGTCCGGTGATGTTGAG For PTS2 AdaptorF

HU2647 AGAAAGCTGGGTGCCATCCTCCACCACCACCGC Rev PTS2 AdaptorR

HU2648 AAAAAGCAGGCTTCGCGTCCGGTGATGTTGAGTATC For PTS1 AdaptorF

HU2649 AGAAAGCTGGGTTTACCATCCTCCACCACCAC Rev PTS1 AdaptorR

At2g28490.1

HU3438 AAAAAGCAGGCTATGGAGAAAAACAAGAGAGC For PTS2 AdaptorF

HU3439 AGAAAGCTGGGTGAACAAAGCGGTCGCTAACCATTTC Rev PTS2 AdaptorR

At2g30970.1

HU3440 AAAAAGCAGGCTATGGCTTTGGCGATGATGATC For PTS2 AdaptorF

HU3441 AGAAAGCTGGGTGAGATGACTTGGTGACTTCATG Rev PTS2 AdaptorR

At2g31670.1

HU2650 AAAAAGCAGGCTTCATATGCGCAAGAATCCGACCGC For PTS1 AdaptorF

HU2651 AGAAAGCTGGGTCTACAGACTAGAAGACTGTG Rev PTS1 AdaptorR

At2g36640.1

HU3656 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCGTCAGACAAAC For PTS1

HU3657 GGGGACCACTTTGTACAAGAAAGCTGGGTCTCACAGCTTTCCCTTAT Rev PTS1

At2g38380.1

HU3009 AAAAAGCAGGCTATGGGGTTTTCTCCTTCATTTTC For PTS2 AdaptorF

HU3010 AGAAAGCTGGGTGGATAGAACTCACAACACCATCG Rev PTS2 AdaptorR

At2g38530.1

HU3442 AAAAAGCAGGCTATGGCTGGAGTGATGAAGTTG For PTS2 AdaptorF

HU3443 AGAAAGCTGGGTGCCTCACGGTGTTGCAGTTGGTG Rev PTS2 AdaptorR

At2g42520.1

HU3027 AAAAAGCAGGCTTCAGTGCATCATGGGCTGATGTAG For PTS1 AdaptorF

HU3028 AGAAAGCTGGGTTTAGTCCCAAGCACTTGGAGG Rev PTS1 AdaptorR

HU3029 AAAAAGCAGGCTATGAGTGCATCATGGGCTGATG For PTS2 AdaptorF

HU3030 AGAAAGCTGGGTGGTCCCAAGCACTTGGAGGTTC Rev PTS2 AdaptorR

At2g47730.1

HU3278 AAAAAGCAGGCTATGGGAGCAATTCAAGCTCGTC For PTS2 AdaptorF

HU3279 AGAAAGCTGGGTGCTGCTTCTGGAGGTCAATAAC Rev PTS2 AdaptorR

At3g11630.1

HU3559 AAAAAGCAGGCTATGGCGTCTGTTGCTTCTTC For PTS2 AdaptorF

HU3560 AGAAAGCTGGGTGAATAGCTGAGAAGTACTCTTTG Rev PTS2 AdaptorR

At3g48170.1

HU3268 AAAAAGCAGGCTTCGCGATTACGGTGCCGAGACG For PTS1 AdaptorF

HU3269 AGAAAGCTGGGTTCAGAGCTTGGAAGGAGGTTTGTAC Rev PTS1 AdaptorR

At3g48730.1

HU3274 AAAAAGCAGGCTTCGCTGCGACGCTTACTGGATC For PTS1 AdaptorF

HU3275 AGAAAGCTGGGTTTAGAGCCGACTTAGAACCTTC Rev PTS1 AdaptorR

At3g55290.1 TOPO

HU3341 CACCGCGATGAGCAATCATCAAACTCAGGT For PTS1

HU3342 TCAGAGAGATGAAAAGATAGGCACACCGGG Rev PTS1

HU3343 GAGAGATGAAAAGATAGGCACACCGGGCAA Rev PTS2 +HU3341

At4g12910.1

HU3266 AAAAAGCAGGCTTCTCAATAATCACAATGGTTTG For PTS1 AdaptorF

HU3267 AGAAAGCTGGGTTTAAATCTTGCTTCCTTCTAGAAATC Rev PTS1 AdaptorR

At4g18360.1

HU3276 AAAAAGCAGGCTTCGAGATAACAAACGTGATGGAATATG For PTS1 AdaptorF

HU3277 AGAAAGCTGGGTCTACAGCTTGGCCGAGAGGTAATG Rev PTS1 AdaptorR

At4g20360.1

HU3565 AAAAAGCAGGCTTCGCGATTTCGGCTCCAGCCGC For PTS1 AdaptorF

HU3566 AGAAAGCTGGGTTCATTCGAGGATCGTCCCAATAAC Rev PTS1 AdaptorR

HU3567 AAAAAGCAGGCTATGGCGATTTCGGCTCCAGC For PTS2 AdaptorF

HU3568 AGAAAGCTGGGTGTTCGAGGATCGTCCCAATAAC Rev PTS2 AdaptorR

At4g34200.1

HU3658 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGTCAGCCACCGCC For PTS1

HU3659 GGGGACCACTTTGTACAAGAAAGCTGGGTCCTAGAGCTTGAGGAA Rev PTS1

At4g36880.1

HU2525 AAAAAGCAGGCTTCGCTCCTTCAACAAAAGTTC For PTS1 AdaptorF

HU2526 AGAAAGCTGGGTTCAAACACTGCTGATAGTATTTC Rev PTS1 AdaptorR

At4g39260.1

HU2638 GGGGACAAGTTTGTACAAAAAAGCAGGCTATGTCTGAAGTTGAGTACCG For PTS1

HU2639 GGGGACCACTTTGTACAAGAAAGCTGGGTCTTACCAGCCGCCACCACCGC Rev PTS1

HU2640 GGGGACCACTTTGTACAAGAAAGCTGGGTCCCAGCCGCCACCACCGC Rev PTS2 +HU2638

At5g16370.1

HU3025 AAAAAGCAGGCTTCGAGCAAATGAAGCCATGCGC For PTS1 AdaptorF

HU3026 AGAAAGCTGGGTTTACATCCGACTCAACCTCGTC Rev PTS1 AdaptorR

At5g17920.1

HU1840 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCTTCACACATTGTTGG For PTS1

HU1841 GGGGACCACTTTGTACAAGAAAGCTGGGTCTCACTTGGCACTGGCGAG Rev PTS1

At5g19550.1

HU3430 AAAAAGCAGGCTATGGATTCCGTCTTCTCTAAC For PTS2 AdaptorF

HU3431 AGAAAGCTGGGTGGCCGAGGCGGGTCACTGCAGC Rev PTS2 AdaptorR

At5g44020.1

HU2112 AAAAAGCAGGCTATGGCTAGATCCTTGTTGCTCTC For PTS2 AdaptorF

HU2113 AGAAAGCTGGGTGGGCGACATAGTAGATGGAGTTAGG Rev PTS2 AdaptorR

At5g46290.1 TOPO

HU3371 CACCGCGATGCAAGCTCTTCAATCTTCATCT For PTS1

HU3372 TCAGGGTTTGAAGGCAGAGAAGGCGACTAC Rev PTS1

HU3373 GGGTTTGAAGGCAGAGAAGGCGACTACCGA Rev PTS2 +HU3371

At5g48230.2 TOPO

HU3191 CACCGCGATGGCCCATACATCAGA For PTS1

HU3192 TCAAAGGAGCTCAAGAAC Rev PTS1

HU3193 AAGGAGCTCAAGAACTAG Rev PTS2 +HU3191

AdaptorF GGGGACAAGTTTGTACAAAAAAGCAGGCT For -

AdaptorR GGGGACCACTTTGTACAAGAAAGCTGGG Rev -

Supplemental Table XVI. Primers used for genotyping and RT-PCR analysis.

P1 and P2 were used for RT-PCR; P3 and P4 were used for genotyping.

name sequence

LB_SALK ATTTTGCCGATTTCGGAAC

LB_SAIL TAGCATCTGAATTTCATAACCAATCTCGATACAC

At1g65880 BZO1 P1 CTCTAACCCCCATGACGTTC

At1g65880 BZO1 P2/P4 GTCCAACCGGCCGTTTAACT

At1g65880 BZO1 P3 GCAGACCGAGGCCACTGGTC

At4g12910 SCPL20 P1 ATCAGCTCCTGAATCTGCTC

At4g12910 SCPL20 P2 CTCCCGCGGTTTGTACTCAG

At4g12910 SCPL20 P3.1 CCGCGGTTGACTCTCACGCG

At4g12910 SCPL20 P4.1 ACGAACTGGCCACGCTCGAC

At4g12910 SCPL20 P3.2 GAGGGCATGGATATCAAATGA

At4g12910 SCPL20 P4.2 TGGAAATGGACCACAAAGGC

At4g18360 GOX3 P1 TGCATCTGGTGCTGAAGATC

At4g18360 GOX3 P2/P4 CTTGGCCGAGAGGTAATGAG

At4g18360 GOX3 P3 TGTCAAGGGTGTTATTACAGCT

At4g36880 RDL1 P1/P3 GCAAGGAGGTTCCAGAGAC

At4g36880 RDL1 P2/P4 CCGGATTTGGAGGCTGCC

At1g13440 GAPC2 P1 CACCACCGAGTACATGACATACATGTTT

At1g13440 GAPC2 P2 TCTTCTGAGTAGCAGTGATAGAGTGGACAG

At1g13440 GAPC2 P3 AATGGTTGGAGTAATGTTGCTG

At1g13440 GAPC2 P4 TGTCTGCAACAAATCGATACC

Documents

Supplemental Material, Quan et al. contains: Supplemental ... · Supplemental Material, Quan et al. contains: Supplemental Figures S1 to S7 Supplemental Document 1 and 2 Supplemental