Super-resolution-nanoscopy Resolvable volumes obtained with
current commercial super-resolution microscopes. Schermelleh L et
al. JCB 2010;190:165-175
Slide 2
Super-resolution imaging principles. Schermelleh L et al. JCB
2010;190:165-175
Slide 3
Super-resolution microscopy of biological samples. Schermelleh
L et al. JCB 2010;190:165-175
Slide 4
Several techniques that produce images with resolution better
than the confocal microscope. Lidke D S, and Lidke K A J Cell Sci
2012;125:2571-2580 4PI2 two opposing lenses ~100-nm axial
resolution (right) reveals mitochondrial fragmentation that was not
apparent in confocal microscopy images (center) SIM- series of
images are collected while scanning the illumination pattern across
the sample 3D images of nuclear pore complex epitopes (red) nuclear
lamina (green). DNA(blue) Resolution 100 by 300nm Bessel beam swept
through the sample 300-nm isotropic resolution combined faster
imaging dramatically reduced photo-bleaching confocal microscopy
(top center) and Bessel beam imaging (bottom center). Single 3D
frame(right) bar=10 m. 3D STED with 50 by100-nm resolution. Images
of neurofilaments. Confocal (center) & 3D STED (right).
Slide 5
Sub-diffraction imaging through photobleaching microscopy Works
for data collected from confocal or wide-field microscope
