Anthony K. L. Leung’s CV Page 1 of 19

SUMMARY ABOUT DR. ANTHONY K. L. LEUNG Dr. Anthony Leung—a scientist and an inventor—is an expert in the area of RNA, ADP-ribosylation and proteomics. Dr. Leung completed his four-year Master in Biochemistry degree at the Exeter College, University of Oxford. Funded by the Croucher Scholarship from Hong Kong and the Overseas Research Scheme Award from the United Kingdom, Dr. Leung earned his Ph.D. in Biochemistry from the University of Dundee under the tutelage of Dr. Angus I. Lamond. Using quantitative proteomics and imaging approaches, Dr. Leung characterized the nucleolar proteome dynamics and discovered a novel nucleolar localization pathway during his Ph.D. In 2004, Dr. Leung was awarded a Human Frontier Science Program Long-term Fellowship and, subsequently in 2007, a Special Fellowship from The Leukemia & Lymphoma Society to perform postdoctoral research on microRNAs under the mentorship of Nobel Laureate Dr. Phillip A. Sharp at MIT. In 2010, Dr. Leung obtained a joint postdoctoral appointment with Dr. Paul Chang at MIT on investigating a therapeutically important protein modification called ADP-ribosylation. Dysregulation of ADP-ribosylation metabolism results in neurodegenerative disorders, virus infection and cancer susceptibility.

Dr. Leung started his lab in 2011 as an Assistant Professor, and currently an Associate Professor, in the Department of Biochemistry and Molecular Biology at Johns Hopkins University. Funded by an Idea Award from the Department of Defense Breast Cancer Research Program, Dr. Leung developed a novel non-biased technique to identify the sites of ADP-ribosylation—a technical breakthrough for the field. He was since then recognized with numerous accolades: Top 5 Agilent Early Career Professor (2013), the Inaugural Johns Hopkins Catalyst Award (2015), Research Scholar Award from the American Cancer Society (2016), and Top 10 Finalist for the American Society of Cell Biology–Gibco Emerging Leader Prize (2016). Dr. Leung has authored over 40 peer-reviewed publications, invited book chapters and review articles. Because of his expertise, Dr. Leung has been frequently invited to review for scientific journals, serve on editorial boards, chair scientific sessions and speak at international meetings. Dr. Leung is highly collaborative on multidisciplinary projects and grants with experts in virology (Dr. Diane Griffin), oncology (Dr. Ben Park), chemistry (Dr. Marc Greenberg), biophysics (Dr. Sua Myong), proteomics (Dr. Shao-En Ong) and single molecule diagnostics (Dr. Al Celedon; Scanogen Inc.). In addition, Dr. Leung actively engages the public with science by initiating direct conversations between basic scientists and cancer survivors.

Read more about our work on public outreach: http://magazine.jhsph.edu/2017/spring/features/the-patient-researcher/

Anthony K. L. Leung’s CV Page 2 of 19

RESEARCH CONTRIBUTIONS BY DR. ANTHONY K. L. LEUNG 1. Created the first map of the dynamic proteome of the human nucleolus (2002–2006)

Dr. Leung’s graduate work focused on proteomic analyses of the nucleolus. As part of an international team co-led by Dr. Angus Lamond, his mentor, and Dr. Matthias Mann, Dr. Leung aimed to understand how nucleolar proteome changes upon stress. This international team not only developed the first proteome map of the nucleolus, but also analyzed how proteins move in and out of the nucleolus under stress. This was the first-of-its-kind proteomics study to measure the fluxes of ~500 proteins in a single experiment (a Nature top 10 article, 2005). Given the high complexity of resultant datasets, Dr. Leung led the informatics effort to analyze the compositional changes in the nucleolus. This and other informatics efforts culminated in a database, which has been continually updated and widely used in the scientific community to date (current version: http://www.peptracker.com). In addition, through characterization of the nucleolar proteome, Dr. Leung also discovered a novel nucleolar targeting pathway (a Cover Story in Journal of Cell Biology, 2002) and analyzed how these non-membraneous RNA granules are broken down and re-formed during mitosis.

2. Defined endogenous localizations and targets of microRNA activities (2006–2011)

MicroRNAs are a large group of ~22 nucleotide-short, non-coding RNAs that regulate fundamental biological processes, including proliferation, differentiation, cell death and stress responses. As a postdoctoral fellow with Dr. Phillip Sharp, Dr. Leung discovered that, contrary to the popular belief then, the majority of microRNA activities are in the diffuse cytoplasm, rather than in a specific class of non-membranous RNA granules called P-bodies. In addition, Dr. Leung identified a change in microRNA localization and activities upon stress conditions, leading to the discovery of novel roles of ADP-ribosylation in the cytoplasm (see section 3 below). Though thousands of human microRNAs had been identified and their gene targets predicted at that time, there were no established methods to identify bona fide targets inside cells. To tackle this challenge, Dr. Leung used biochemical methods together with next-generation sequencing to purify microRNA targets. Using mouse embryonic stem cells as a model, Dr. Leung employed informatics analyses to narrow down a highly validated set of ~300 microRNA targets, where 17 out of 18 targets were verified positive (an Article of the Month in Nature Structural and Molecular Biology, 2011). 3. Discover novel roles of ADP-ribosylation in RNA biology (2011–)

ADP-ribosylation is regulated by 17 poly(ADP-ribose) polymerases (PARPs). Since its discovery in 1963, ADP-ribosylation has been implicated in regulating fundamental DNA metabolic processes, including DNA repair, transcription and maintaining chromatin structures in the nucleus. However, relatively little is known about the role of ADP-ribosylation in RNA metabolism or in the cytoplasm. Of note, ADP-ribosylation is therapeutically important as evidenced by multiple Phase III clinical trials ongoing for cancer patients on drugs that inhibit the catalytic activity of PARP-1, including three recently approved by the FDA. However, many of these PARP inhibitors can cross-react with cytoplasmic PARPs. To date, functions of most human PARPs remain unclear, thus identifying their functions represents a major priority and could have strong therapeutic implications. While investigating the role of microRNAs in stress as a postdoc, Dr. Leung found that the core microRNA-binding protein Argonaute is ADP-ribosylated and such ADP-ribosylation reduces microRNA activities. In addition, Dr. Leung is the first to note that ADP-ribosylation, 5 PARPs, along with degradative enzymatic counterparts, are enriched in non-membranous RNA granules in the cytoplasm called stress granules (a Cover Story in Molecular Cell, 2011). Establishing his lab at Johns Hopkins University, Dr. Leung used informatics analyses to further show that RNA granule proteins enriched for low complexity regions, which aid self-assembly of nonmembranous structures, are preferentially ADP-ribosylated, indicating how ADP-ribosylation could direct cellular organization. Recently, Dr. Leung also found that a subset of RNA viruses encodes a macrodomain that removes (hydrolyzes) ADP-ribosylation. Mutants abrogating such ADP-ribosylhydrolase activity are not viable whereas mutants reducing the activity have slower replication in mammalian cells and reduced virulence in mice. The study on RNA viruses will provide an outstanding biological context to investigate the role of ADP-ribosylation in the cytoplasm. 4. Develop novel tools to profile ADP-ribosylated proteome (2014–)

Though ADP-ribosylation was discovered over 50 years ago, it has been challenging to answer mechanistic questions without knowing where the endogenous sites of modification are. Previously, ADP-ribosylation sites could only be inferred by mutagenesis, but the deficiency of ADP-ribosylation in such site-specific mutants can also be explained by secondary effects. Recently, Dr. Leung’s lab has successfully developed a non-biased proteomics approach to identify ADP-ribosylation sites at all classes of amino acids at the proteome level inside cells. By comparison with other methods developed at the same time, his method is unique in that it identifies modifications on any amino acid. Dr. Leung’s team also

Anthony K. L. Leung’s CV Page 3 of 19

recently developed the first publicly available database of ADP-ribosylated proteins (http://ADPriboDB.leunglab.org) for researchers inside and outside the field to appreciate the complexity and breadth of this therapeutically important protein modification. 5. Collaborate with scientists across disciplines in developing biomedical tools for clinical translation (2009–)

Besides basic biological research, together with collaborators from different disciplines, we develop nanoparticles to deliver siRNAs and other tools that can be translated in clinics. Our work on poly(ADP-ribose) in RNA granules and microRNA activities was recognized for their therapeutic potentials by the granting of two US patents. A provisional patent has also recently been filed for a method to identify ADP-ribosylated sites in proteins.

Anthony K. L. Leung’s CV Page 4 of 19

date revised 07/13/2018

CURRICULUM VITAE Anthony K. L. Leung

PERSONAL DATA Business Address Johns Hopkins University Bloomberg School of Public Health 615 N. Wolfe Street, E8632 Baltimore, MD 21205 Phone: 410 502-8939 Email: anthony.leung@jhu.edu URL: www.leunglab.org Facebook: www.fb.com/leunglab Twitter: www.twitter.com/leunglab (@leunglab) EDUCATION AND TRAINING Sep 1995–May 1999 Master in Biochemistry (4-year undergraduate degree), University of Oxford, UK

Sep 1999–Mar 2003 Ph.D. in Biochemistry, University of Dundee, UK Advisor: Professor Angus I. Lamond Thesis: Proteomics and Dynamics of the Human Nucleolus

Summers 2002/2003 Visiting Scientist, European Molecular Biology Laboratory, Heidelberg, Germany

Apr 2004–Jun 2009 Postdoctoral Fellow, Laboratory of Phillip A. Sharp, Massachusetts Institute of Technology, USA

Oct–Nov 2005 Course Attendee, Immunocytochemistry, in situ hybridization and live cell imaging, Cold Spring Harbor Laboratory, USA

Jul 2009–Jun 2010 Senior Postdoctoral Fellow, Laboratory of Phillip A. Sharp, Massachusetts Institute of Technology, USA with a secondary appointment at the laboratory of Paul Chang, MIT since Jan 2010

Jul 2010–Jan 2011 Research Scientist, Laboratory of Phillip A. Sharp, Massachusetts Institute of Technology, USA

Nov 2016– Executive Education Course Attendee, Johns Hopkins Carey Business School Strategic Team Leadership (11/16), Persuasive Communication (3/17), Strategic Negotiation (9/17), Project Management (3/18), Technology Entrepeurship (7/18)

PROFESSIONAL EXPERIENCE 2011– 2017 Assistant Professor, Johns Hopkins University, Bloomberg School of Public Health (JHSPH),

Department of Biochemistry and Molecular Biology (BMB)

2011– Preceptor, predoctoral training program in Cancer Research (T32CA009110), Bloomberg School of Public Health, Johns Hopkins University

2011– Affiliate Member, McKusick-Nathans Institute of Genetic Medicine (IGM), Johns Hopkins School of Medicine (JHSOM)

2011– Preceptor, predoctoral training program in Human Genetics (T32GM007814), JHSOM

2015– Joint appointment in Department of Oncology, School of Medicine, Johns Hopkins University

2015– Member, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins

2015– Preceptor, pre-doctoral training program in Chemistry–Biology Interface (CBI, T32GM080189), Johns Hopkins University

Anthony K. L. Leung’s CV Page 5 of 19

2017– Associate Professor, Johns Hopkins University, Bloomberg School of Public Health (JHSPH), Department of Biochemistry and Molecular Biology (BMB)

2018– Joint appointment in Department of Molecular Biology and Genetics, School of Medicine, Johns Hopkins University

2018– Preceptor, pre-doctoral training program in Biochemistry, Cellular and Molecular Biology graduate program (BCMB, T32GM007445), School of Medicine Johns Hopkins University

PROFESSIONAL ACTIVITIES Society Memberships: RNA Society (2011– ) American Society of Cell Biology (2016– ) American Society of Virology (2018– ) Invited activities: Plenary Luncheon Seminar, RNA meeting 2011 Kyoto, Japan

Keynote Speaker, Genomics 2013, Boston, Massachusetts

Session Chair, FASEB Meeting "NAD+ Metabolism and Signalling 2015”, Germany.

Invited Speaker, Cold Spring Harbor Laboratory meeting on the PARP Family and ADP-ribosylation, USA (2016).

Session Chair, PARP2017, 20th International Conference on ADP-ribosylation, Hungary.

Session Chair, FASEB Meeting "NAD+ Metabolism and Signalling 2017”, USA.

Session Chair, Stress-Associated RNA Granules in Human Disease and Viral Infection (2nd International Symposium), Heidelberg, Germany, 2017

Founding member, FEBS Network on ADP-ribosylation and NAD+ metabolism (2018– )

Co-organizer, Workshop on the Biology, Chemistry and Physics of non-membranous granules at Johns Hopkins (with Drs. Taekjip Ha and Takanari Inoue; September 2016)

EDITORIAL ACTIVITIES 2014– Member, Editorial Board Scientific Reports (Nature Publishing group) 2011– Member, Editorial Board

Frontiers in Genetics (Nature Publishing group) 2011– Ad hoc Reviewers for PLoS Pathogen, Microbiology and Molecular Biology Reviews, Nucleic Acids Research, Biochemistry, Scientific

Reports, PLoS genetics, Bioorganic and Medicinal Chemistry Letters, BMC Genomics, Journal of Cell Science, Journal of Cell Biology, EMBO Report, EMBO Journal, Chemical Reviews, FEBS Journal, Molecular and Cellular Biology, Journal of Proteomics, Nature Communications, ACS Chemical Biology, Nature Chemical Biology, Cell Chemical Biology, Nature Protocol, Cell Report, PLoS Biology, PNAS, JCI Insight, Molecular Cell, Cell, Science

2011 Textbook Manuscript Reviewer on Chapter 11: Protein Modification and Targeting

Molecular Biology: Principles of Genome Function (2nd edition), by Nancy Craig, Orna Cohen-Fix, Rachel Green, Carol Greider, Gisela Storz, and Cynthia Wolberger Oxford University Press (ISBN: 978-0-19-956206-0)

Ad Hoc Grant Reviewer for

Chinese University of Hong Kong (2005), Royal Society, United Kingdom (2006), Human Frontier Science Program (2014).

Anthony K. L. Leung’s CV Page 6 of 19

Netherlands Organisation for Scientific Research (NWO) (2015, 2016) Biotechnology and Biological Sciences Research Council (BBSRC), United Kingdom (2015) National Science Center (Narodowe Centrum Nauki), Poland (2016) Israel Science Foundation, Israel (2016) Health and Medical Research Fund from The Government of the Hong Kong Special Administrative Region (HKSAR) (2016–2018) Canada Research Chairs (2017) Intramural NIH/NIA Board of Scientific Counselors’ meeting (2017) Qiu Shi Outstanding Young Scholar Award, People Republic of China (2018) Agence nationale de la recherche, France (2018)

Poster judge for

9th Frontiers in Chemistry and Biology Interface Symposium, Baltimore, MD (2016) 12th Annual Retreat for Johns Hopkins Chemistry–Biology Interface Graduate Program (2016) PARP2017 meeting (2017) FASEB NAD+ metabolism and Signalling meeting (2017) Johns Hopkins Postdoctoral Association Annual Retreat (2018)

HONORS AND AWARDS

1995–1999 Waugh Scholarship, University of Oxford, UK 1995–1999 Fitzgerald Prize, University of Oxford, UK 1999–2002 Overseas Research Scheme Award, UK 1999–2002 Scholarship, Croucher Foundation, Hong Kong Jun–Jul 2002 European Advanced Light Microscopy Facility Fellowship, EMBL Heidelberg, Germany 2004–2007 Long-term Fellowship, Human Frontier Science Program 2007–2010 Special Fellowship, The Leukemia & Lymphoma Society 2011–2013 Idea Award, Department of Defense Breast Cancer Research Program 2013 Top 5 Finalist, Agilent Early Career Professor Award 2013–2014 Faculty Innovation Award, Johns Hopkins Bloomberg School of Public Health 2015 Inaugural Johns Hopkins Catalyst Award 2016 American Cancer Society Research Scholar Award 2016 Top 10 Finalist, American Society of Cell Biology (ASCB)–Gibco Emerging Leader Prize 2017 Finalist, Burroughs Wellcome Fund Investigators in the Pathogenesis of Infectious Disease 2017 Johns Hopkins Discovery Award 2017 W. W. Smith Charitable Trust Medical Research Award 2017 Teaching Excellence Award, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD

(Term 1) 2017

Awards for Leung Lab members

2012 Journal of Cell Science Travelling Fellowship to PhD student, Casey Daniels. 2013 PhD student Casey Daniels attended Proteomics Course in Cold Spring Harbor Laboratory with

scholarship support. 2013 Postdoctoral Fellow Dr. Yoshinari Ando was awarded a Travel Grant Award to attend 36th Annual

Meeting of the Molecular Biology Society of Japan. 2014 Elsa Orent Keiles Fellowship in Biochemistry to PhD student, Casey Daniels. 2014 Joy Cappel Young Investigator Award from the Rockland Immunochemicals Inc. to PhD student,

Casey Daniels. 2015 Postdoctoral Fellow Dr. Yoshinari Ando was awarded a Traveling Fellowship, 20th Annual Meeting,

RNA Society (2015). 2015 Journal of Cell Science Travelling Fellowship to PhD student, Lyle McPherson. 2015 Joy Cappel Young Investigator Award from the Rockland Immunochemicals Inc. to Postdoctoral

Fellow, Dr. Hyunju Ryu. Winners have been selected based on their commitment and contributions to innovative and collaborative scientific achievements and advances in the life sciences for promising post-doctoral fellows and graduate students.

Anthony K. L. Leung’s CV Page 7 of 19

2016 John Scocca Achievement Award to PhD student, Casey Daniels, for excellence in bench research on the part of a PhD student in the Department of Biochemistry and Molecular Biology, JHSPH.

2017 Elsa Orent Keiles Fellowship in Biochemistry to PhD student, Lyle McPherson. The fellowship is to recognize a PhD student who has performed admirably in the academic and research programs of the department of Biochemistry and Molecular Biology, JHSPH, and sets an example for his/her fellow students. One student is selected to receive this award each year.

2017 Best Talk Award, Annual Retreat, Department of Biochemistry and Molecular Biology to PhD student, Lyle McPherson.

2017 Best Poster Award, PARP2017, 20th International Conference on ADP-ribosylation, to PhD student, Lyle McPherson.

2018 Poster Award, 2nd place, 2018 Genetics Research Day Poster Competition from the Maryland Genetics, Epidemiology and Medicine Training Program to PhD student, Joe Fischer

2018 Ruth L. Kirschstein National Research Service Award Individual Predoctoral Fellowship (F31) from National Institute of General Medical Sciences at National Institute of Health to PhD student, Joe Fischer

2018 Best Talk Award, Annual Retreat, Department of Biochemistry and Molecular Biology to PhD student, Joe Fischer.

2018 Best Poster Award, Annual Retreat, Department of Biochemistry and Molecular Biology to PhD student, Lyle McPherson.

2018 Co-awardee, Fisher Center Discovery Program Pilot Grant to Postdoctoral Fellow, Aravinthkumar Jayabalan

2018 Winner in Research Scientist/Postdoctoral Fellow Category, Research Photo Context, Johns Hopkins University to Postdoctoral Fellow, Aravinthkumar

2018 Ministry of Education Scholarship, Taiwan to PhD student, Shang-Jung Cheng

PUBLICATIONS * indicates corresponding authorship; #indicates co-first authorship; underlined indicates other Leung Lab member Publications cited at least 10 times (as of July 13th, 2018) are denoted below. Detailed citation information can be found (Citation: 5672; h-index=26): https://scholar.google.com/citations?user=h_vjSXoAAAAJ&hl=en Original Research

1. Kolatkar AR, Leung AK, Isecke R, Brossmer R, Drickamer K, Weis WI (1998). Mechanism of N-acetylgalactosamine binding to a C-type animal lectin carbohydrate recognition domain. J Biol Chem 273:19502-8. (cited 93 times)

2. Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (2002). Directed proteomic analysis of the human nucleolus. Curr Biol 12(1): 1-11. (cited 997 times)

3. Fox AH, Lam YW, Leung AK, Lyon CE, Andersen J, Mann M, Lamond AI (2002). Paraspeckles: A novel nuclear domain. Curr Biol 12:13-25. (cited 382 times)

4. Oeffinger M, Leung A, Lamond A, Tollervey D (2002). Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis. RNA 8:626-36. (cited 84 times)

5. Leung AK, Lamond AI (2002). In vivo analysis of NHPX reveals a novel nucleolar localization pathway involving a transient accumulation in splicing speckles. J Cell Biol 157:615-29. (cited 59 times)

6. Leung AK, Andersen JS, Mann M, Lamond AI (2003). Bioinformatic analysis of the nucleolus. Biochem J 376(Pt 3):553-569. (cited 163 times)

7. Leung AK, Gerlich D, Miller G, Lyon C, Lam YW, Lleres D, Daigle N, Zomerdijk J, Ellenberg J, Lamond AI (2004). Quantitative kinetic analysis of nucleolar breakdown and reassembly during mitosis in live human cells. J Cell Biol 166(6):787-800. (cited 158 times)

8. Chen D, Dundr M, Wang C, Leung A, Lamond A, Misteli T, Huang S (2005). Condensed mitotic chromatin is accessible to transcription factors and chromatin structural proteins. J Cell Biol 168(1):41-54. (cited 195 times)

9. Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (2005). Nucleolar proteome dynamics. Nature 433(7021):77-83. (cited 1044 times)

Anthony K. L. Leung’s CV Page 8 of 19

10. Leung AK, Trinkle-Mulcahy L, Lam YW, Andersen JS, Mann M, Lamond AI (2006). NOPdb: Nucleolar Proteome Database. Nucleic Acids Res 34 (Database issue): D218-220. (cited 109 times)

11. Leung AK, Calabrese MJ, Sharp PA (2006). Quantitative analysis of Argonaute protein reveals microRNA-dependent Localisation to Stress Granules. Proc Natl Acad Sci USA 103:18125-30. (cited 340 times)

12. Dafik L, Kalsani V, Leung AK, Kumar K (2009). Fluorinated lipids permit facile passage of macromolecules into living cells. J Am Chem Soc 131(34):12091-3. (cited 17 times)

13. Singh N, Agrawal A, Leung AK, Sharp PA, Bhatia S (2010). Effect of nanoparticle conjugation on gene silencing by RNA interference. J Am Chem Soc 132(24):8241-3. (cited 80 times)

14. Leung AK #, Young GA #, Bhutkar AJ, Zheng GX, Sharp PA (2011). Genomewide Identification of Endogenous Argonaute Binding sites in Mouse Embryonic Stem Cells with and without Mature microRNAs. Nature Struct. Mol. Biol. 18:237-44. (cited 221 times)

15. Leung AK, Vyas S, Rood JE, Bhutkar AJ, Sharp PA, Chang P (2011). Poly(ADP-ribose) Regulates microRNA activity and stress responses in the Cytoplasm. Mol Cell 42:489-99. (cited 229 times)

16. Daniels CM, Ong SE*, Leung AK* (2014). Phosphoproteomic Approach to Characterize Protein Mono- and Poly(ADP-ribosyl)ation Sites from Cells. J Proteome Res. 13:3510-22. (cited 63 times)

17. Daniels CM, Thirawatananond P, Ong SE*, Gabelli SB*, Leung AK* (2015). Nudix hydrolases degrade protein-conjugated ADP-ribose. Sci Rep. 5:18271. (cited 24 times)

18. Hwang T, Park CK, Leung AK, Gao Y, Hyde TM, Kleinman JE, Rajpurohit N, Tao R, Shin JH, Weinberger DR (2016). Dynamic regulation of RNA editing in human brain development and disease. Nature Neuroscience 19(8):1093–9. (cited 39 times)

19. Palazzo L#, Daniels CM#, Nettleship JE, Rahman N, McPherson RL, Ong SE, Kato K, Nureki O, Leung AK*, Ahel I* (2016). ENPP1 processes protein ADP-ribosylation in vitro. FEBS. J. 283(18):3371–88. (cited 16 times)

20. Vivelo CA#, Wat R#, Agrawal C, Tee HY, Leung AK* (2017). ADPriboDB: The Database of ADP-ribosylated Proteins. Nucleic Acids Research 45(D1):D204-D209; ePub Date: Aug 2016. http://ADPriboDB.leunglab.org/ (cited 11 times)

21. McPherson RL#, Abraham R#, Sreekumar E#, Ong SE, Cheng SJ, Baxter V, Kistemaker HAV, Filippov DV, Griffin DE*, Leung AK* (2017). ADP-ribosylhydrolase activity of macrodomain is required for Chikungunya viral replication and virulence. Proc Natl Acad Sci USA 114(7):1666-1671. (cited 22 times)

22. Das S, Kohr M, Dunkerly B, Bedja D, Kent OA, Leung AK, Henao-Mejia J, Henao-Mejia J, Flavell RA, Steenbergen C (2017). Role of miR-181 Family in the Heart: A Tale of Two Intracellular Compartments. J Am Heart Assoc. 6: e004694

23. Zhang T, Wu Y-C, Mullane P, Ji YJ, Liu H, He L, Arora A, Hwang H-Y, Alessi AF, Niaki AG, Periz G, Guo L, Wang H, Elkayam E, Joshua-Tor L, Myong S, Kim J, Shorter J, Ong SE, Leung AK, Wang J (2018). FUS regulates activity of microRNA-mediated Gene Silencing. Mol Cell., 69(5):787-801.

Review Articles

24. Leung AK, Lamond AI (2003). The Dynamics of the Nucleolus. Crit Rev Eukaryot Gene Expr 14:39-54. (cited 92 times)

25. Leung AK, Sharp PA (2006). Function and Localization of microRNAs in mammalian cells. Cold Spring Harb Symp Quant Biol 71:29-38. (cited 80 times)

26. Leung AK, Sharp PA (2007). microRNAs: A safeguard against Turmoil? Cell 130:581-5. (cited 225 times) 27. Leung AK, Sharp PA (2010). MicroRNA Functions in Stress Responses. Mol. Cell 40:205-15. (cited 565 times) 28. Leung AK*, Todorova T, Ando Y, Chang P (2012). Poly(ADP-ribose) regulates post-transcriptional gene regulation

in the cytoplasm. RNA Biol. 9(5): 542-548. (cited 43 times) 29. Leung AK* (2014). Poly(ADP-ribose): An organizer of cellular architecture. J. Cell Biol. 205:613-9. (cited 61 times) 30. Vivelo CA, Leung AK* (2015). Proteomics approaches to identify mono-(ADP-ribosyl)ated and poly(ADP-

ribosyl)ated proteins. Proteomics 15(2-3):203-17. (cited 19 times) 31. Daniels CM, Ong SE, Leung AK* (2015). The Promise of Proteomics for the Study of ADP-Ribosylation. Mol Cell

58(6):911-24. (cited 67 times) 32. Leung AK* (2015) The Whereabouts of MicroRNA Actions: Cytoplasm and Beyond. Trends Cell Biol. 25:601-610.

(cited 48 times) 33. Fischer J, Leung AK* (2017). CircRNAs: A Regulator of Cellular Stress. Critical Review in Biochemistry and

Molecular Biology 52: 220-233. (cited 15 times) 34. Leung AK* (2017). PARPs. Curr. Biol. 27(23), R1256-8.

Anthony K. L. Leung’s CV Page 9 of 19

35. Leung AK*, McPherson RL, Griffin DE* (2018). Macrodomain ADP-ribosylhydrolase and the Pathogenesis of Infectious Diseases. PLoS Pathog. 14(3): e1006864.

Previews

36. Ando Y, Leung AK* (2013). Does an emergency visit to the ER make microRNAs stronger during stress? Mol. Cell 52: 1-3.

37. McPherson RL, Leung AK* (2016). ADPr-ChAP: Mapping ADP-Ribosylation onto the Genome. Mol Cell 61(3):327-8.

38. Leung AK* (2017). SERious Surprises for ADP-ribosylation Specificity: HPF1 Switches PARP1 Specificity to Ser Residues. Mol Cell 65:777-778.

Book Chapters, Monographs

39. Lam YW, Fox AH, Leung AK, Andersen J, Mann M, Lamond AI (2004). Nucleolar Proteomics. The Nucleolus. Olson, Mark O.J. (Ed) Landes Bioscience.

40. Rood JE, Leung AK, Chang P (2011). Strategies for purification of proteins associated with cellular poly(ADP-ribose) and PARP-specific poly(ADP-ribose). Methods in Molecular Biology 780:153-164.

41. Leung AK* (2012). MicroRNA Functions in Stress Responses. Continued Education Coursebook for the Society of Toxicology.

42. Leung AK* (2012). Overview of microRNAs in Toxicology and Human Diseases. Continued Education Coursebook for the Teratology Society. (2012)

43. Leung AK*, Sharp PA (2013). Quantifying Argonaute in and out of GW/P-bodies: Implication in microRNA silencing. Adv Exp Med Biol. 768:165-182. (cited 32 times)

44. Ando Y, Leung AK* (2015). Methods for studying microRNA functions during stress. Methods Mol Biol 1292:115-28

45. Fan AC, Leung AK* (2016). RNA Granules and Diseases — A Case Study of Stress Granules in ALS and FTLD. Adv Exp Med Biol 907:263–96.

46. Daniels CM, Ong SE, Leung AK* (2017). ADP-ribosylated peptide enrichment and site identification: The phosphodiesterase-based method. Methods in Molecular Biology 1608:79-93

47. Abraham R, McPherson RL, Sreekumar E, Leung AK*, Griffin DE*. Preparation of recombinant Alphaviruses for functional studies of ADP- ribosylation. Methods in Molecular Biology, in press

48. McPherson RL, Ong SE, Leung AK*. Quantitative determination of MAR hydrolase residue specificity in vitro by tandem mass spectrometry. Methods in Molecular Biology, in press

Patents

1. Methods and Compositions for increasing the activity of inhibitory RNA US 2011/0097328 A1

2. Compositions and Methods for treating cancer and modulating stress granule formation. US 2013/0156776 A1

3. The Use of Escherichia Coli RppH to Identify ADP-ribosylated Sites by Mass Spectrometry. Provisional patent US 62/173,554

Anthony K. L. Leung’s CV Page 10 of 19

CURRICULUM VITAE Anthony K. L. Leung

PART II

Student Advisement in Research PhD Students Casey M. Daniels PhD Candidate, 06/11–08/15

Thesis Title: Characterization of the ADP-ribosylated Proteome by Mass Spectrometry Currently, Postdoctoral Fellow with Dr. Aleksandra Nita-Lazar at NIAID

Christina Vivelo PhD Candidate, 06/13–07/18 Thesis Title: Global analysis of ADP-ribosylated substrates and a case study of poly(ADP-ribose) dependent ubiquitination.

Lyle McPherson PhD Student, 04/15– Joe Fischer PhD Student, 06/15– Morgan Dasovich PhD Student, 08/17– (co-mentor: Dr. Marc Greenberg, Department of Chemistry) Shang-Jung Cheng PhD Student, 04/18– Veronica Busa PhD student, 08/18– PhD rotation students JHSPH BMB: Hongfan Chen (2011), Casey Daniels (2011), Chynna Broxton (2011), Xin Sun (2012), Abigail Zieman (2012), Christina Vivelo (2013), Hana Odeh (2013), Pola Olczak (2014), Yajuan Guo (2014), Tianlu Ma (2014), Lyle McPherson (2015), Ed Culbertson (2015), Danielle Bouchard (2016), Shang-Jung Cheng (2017) JHSOM IGM: Donghui Li (2012), Simeon Springer (2013), Joe Fischer (2015), Claire Wenger (2017), Veronica Busa (2018) JHU CBI: Morgan Dasovich (2016), Lauren Bambarger (2017), Shaun Spisak (2017) Masters Students Jennifer A. Pak ScM Candidate, 06/11–01/12 Ryan Weltzer ScM Candidate, 07/12–10/13 Haobo Wang MHS/ScM Candidate, 09/14–08/16 Shang-Jung Cheng MHS/ScM Candidate, 01/16–08/17 Prithvi Sinha MHS Candidate, 08/17–10/17 Wei Wang MHS Candidate, 10/17–03/18 Geervani Daggupati MHS Candidate, 01/18– Ricky Wat MPH Candidate, Johns Hopkins University, Bloomberg School of Public Health, 09/15–06/16 Undergraduate Students Hui Yi Tee BS Molecular & Cellular Biology Candidate, Johns Hopkins University, 11/14–04/16 Emily Burnette BS Biomedical Engineering Candidate, Johns Hopkins University, 09/17– Garth Cook BS Molecular & Cellular Biology Candidate, Johns Hopkins University, 09/17– Kathryn Gauch BS Molecular & Cellular Biology Candidate, Johns Hopkins University, 09/17– Claire Shao BS Molecular & Cellular Biology Candidate, Johns Hopkins University, 09/17– Mark Vdovychenko BS Molecular & Cellular Biology Candidate, Johns Hopkins University, 09/17–12/17 Pat Visanpattanasin BA Biology Candidate, Johns Hopkins University, 09/17– Vinay Ayyappan BS Biomedical Engineering Candidate, Johns Hopkins University, 01/18– External Christine Shen Grade 12 High School student (Marriott Ridge High School, MD)

07/13–08/13 Jayson Baman MD Candidate, University of Rochester, 06/14–08/14

- supported via Offices for Medical Education Summer Research Fund, University of Rochester Medical Center

Anthony K. L. Leung’s CV Page 11 of 19

Alexander Fan BSc Candidate (Bioengineering), University of Colorado, Boulder, 05/14–08/14 Jerome Yu BSc Candidate, Fudan University, China, 06/14–08/14 Charul Agrawal BSc Candidate, Indian Institute of Technology, Delhi

- supported via Khorana Scholarship program, 05/15–07/15 Postdoctoral Fellow Advisement Yoshinari Ando 09/11–08/17, currently Senior Staff Scientist, National Cancer Center, Japan Naoki Hida 08/12–04/13, currently Assistant Specialist II, UC Merced. Hyunju Ryu 06/15–07/17, currently Homemaker Aravinthkumar Jayabalan 10/16–Current Mohsen Badiee 01/18–Current Student Advisement in Teaching MHS Thesis advisor Aditi Kantipuly MHS Candidate, 12/13–04/14 Hema Chagarlamudi MHS Candidate, 12/14–04/15 Stephanie Owusu MHS Candidate, 12/14–04/15 Deasty Imara MHS Candidate, 12/15–04/16 Shae Rowlandson MHS Candidate, 12/15–04/16 Alex Cole MHS Candidate, 12/16–04/17 Amanda Lourenco MHS Candidate, 12/16–04/17 Balaganesh Natarajan MHS Candidate, 12/16–04/17 Bryanna Stukes MHS Candidate, 12/16–04/17 Chase Alston MHS Candidate, 12/17–04/18 Leah Goldberg MHS Candidate, 12/17–04/18 Geervani Daggupati MHS Candidate, 12/17–04/18 MHS advisor Joseph Shen MHS Candidate, 09/15–06/16 Megan Fong MHS Candidate, 09/15–06/16 Megyin Chen MHS Candidate, 09/16–06/17 Jingting Han MHS Candidate, 09/16–06/17 Esteban Urias MHS Candidate, 09/16–06/17 MHS Thesis reader Anthony Chen (BMB, JHSPH, 2011) Ryan Weltzer (BMB, JHSPH, 2012) Haobo Wang (BMB, JHSPH, 2015) Joseph Shen (BMB, JHSPH, 2016) Kaylin Gozalez (BMB, JHSPH, 2016) Wei Wang (BMB, JHSPH, 2017) Mathew Thomas (BMB, JHSPH, 2017) ScM Thesis reader Grace Hwang (BMB, 2012) Advisor: Daniela Drummond-Barbosa Mridula Balakrishnan (BMB, 2012) Advisor: Fengyi Wan Yoo Jin Kim (BMB, 2017) Advisor: Jennifer Kavran Student Advisement in Community Thesis Committee Member Institute of Genetic Medicine, JHSOM Tyler Creamer 2014–2015 Advisor: Dan Warren Joseph Tilghman 2016–Current Advisor: Aravinda Chakravarti

Anthony K. L. Leung’s CV Page 12 of 19

Department of Biomedical Engineering, JHSOM Taeyoung Hwang 2014–2016 Advisor: Daniel Weinberger Department of Pathology, JHSOM Youngran Park 2016–2017 Advisors: Tian-Li Wang and Ie-Ming Shih Chemistry-Biology Interface, JHU Sabrina Schatzman 2016–Current Advisor: Val Culotta Ryan Porell 2017–Current Advisor: Ron Schnaar Department of Biochemistry and Molecular Biology, JHSPH Elizabeth Alexander 2016–Current Advisor: Jiou Wang Stephen Wellard 2017–Current Advisor: Phil Jordan Leah Cairns 2017–Current Advisor: Jennifer Kavran Preliminary Oral Exams Department of Biochemistry and Molecular Biology, JHSPH Shaina L. Palmere Exam Committee Member (2012) Advisor: Daniela Drummond-Barbosa Shuo Li Exam Committee Member (2016) Advisor: Elizabeth Chen Department of Biostatistics, JHSPH Alyssa Frazee Exam Committee Member (2012) Advisor: Jeff Leek Chemistry–Biology Interface, JHU Katelyn Jackson Exam Committee Member (2018)

Advisor: Scott Bailey Institute of Genetic Medicine, JHSOM Melissa Lee Exam Committee Member (2012) Advisor: Garry Cutting Jane Welch Exam Committee Member (2013) Advisor: Sarah Wheelan and

Kathleen Burn Jing You Exam Committee Member (2014) Advisor: Dave Valle Hannah Edelman Exam Committee Member (2015) Advisor: Andrew McCallion Sarah Robbins Exam Committee Member (2016) Advisor: Dave Valle Anna Moyer Exam Committee Member (2017) Advisor: Roger Reeves Lily Zheng Exam Committee Member (2017) Advisor: Rachel Karchin Other Exam Committees Final Thesis Defense/Reader Department of Biostatistics, JHSPH Samara Kiihl Exam Committee Member (2012) Advisor: Rafael Irizarry Alyssa Frazee Exam Committee Member (2014) Advisor: Jeff Leek Department of Environmental Health and Engineering, JHSPH Suengwon Lee Exam Committee Member (2014) Advisor: James Sham Tyna Dao Exam Committee Member (2015) Advisor: Winnie Tang Suzanne N. Martos Exam Committee Member (2017) Advisor: Zhibin Wang Department of Biomedical Engineering, JHSOM Taeyoung Hwang Exam Committee Member (2016) Advisor: Daniel Weinberger Department of Pathology, JHSOM Youngran Park Exam Committee Member (2017) Advisors: Tian-Li Wang and Ie-Ming Shih

Anthony K. L. Leung’s CV Page 13 of 19

1st Thesis Proposal Committee, BMB, JHSPH Daisy Colon-Lopez Exam Committee Member (2011) Advisor: Jürgen Bosch Shaina L. Palmere Exam Committee Member (2011) Advisor: Daniela Drummond-Barbosa Xin Sun Exam Committee Member (2012) Advisor: Fengyi Wan Project Proposal Exam, CBI, JHU Zach Mazanek Exam Committee Member (2016) Advisor: Jungsan Sohn CBI Project Proposal Reader Pinaki Bose (2015) Advisor: Heng Zhu Zach Mazanek (2016) Advisor: Jungsan Sohn Teaching Classroom instruction 2011– Lecturer, Advanced Topics in Human Genetics (JHSOM) 2011– Lecturer, Current Topics in BMB (JHSPH, 120.872.01) 2011– Faculty Leader, Core Research Literature (JHSPH, 120.852.01) 2013– Lecturer, Introduction to Molecular Biology (JHSPH, 120.602.01) 2013– Lecturer, Molecular Biology of Cellular Stress and Quality Control (JHSPH, 120.606.01) 2014 Lecturer, Molecular Mechanism of Disease (JHSOM, ME 710.702) 2014– Co-director, Introduction to Molecular Biology (JHSPH, 120.602.01) 2016– Lecturer, CBI core course II (JHU cross departmental PhD program) 2016– Lecturer, Molecular Toxicology (JHSPH, 187.632.01) 2018 Lecturer, Biophysics 250. 106/300/306 (JHU) Workshops /seminars 3/2012 Lecturer, Continuing Education Course on “MicroRNA Functions in Stress Responses” in the Society of

Toxicology, Annual Meeting, San Francisco, California 6/2012 Lecturer, Continuing Education Course on “MicroRNA Functions”, Teratology Society, Annual Meeting,

Baltimore, Maryland RESEARCH GRANT PARTICIPATION EXTRAMURAL Funding Research Extramural Funding (i) Current 03/01/15–02/29/20 Post-transcriptional Gene Regulation by Cytoplasmic Poly(ADP-ribose) polymerases R01GM104135

Sponsor: NIH/NIGMS Effort: 30%

The main goal of this project is to study the role of PARylation in microRNA binding protein argonaute in virus infection.

07/01/16–06/30/20 Increase microRNA activities by inhibiting poly(ADP-ribose) polymerases Research Scholar Award

Sponsor: American Cancer Society Effort: 25% The main goal of this project is to study the role of PARylation in microRNA binding

protein argonaute in breast cancer.

Anthony K. L. Leung’s CV Page 14 of 19

04/01/18–03/31/19 Dissecting ADP-ribosylation Signals in Tamoxifen Resistance The W. W. Smith Charitable Trust Medical Research Award Sponsor: The W. W. Smith Charitable Trust

Effort: 10% The main goal of this project is to identify the role of MacroD2 in tamoxifen resistance.

(ii) Previous Grants/Contracts 08/01/11–07/31/13 The Role of Poly(ADP-ribose) in microRNA activity in Breast Cancers Idea Award BC101881 Sponsor: Department of Defense Breast Cancer Research Program Effort: 20% The main goal of this project is to identify how AGO2 PARylation is dysregulated in breast

cancer cells through development of novel proteomics tools. 07/01/15–06/30/16 Novel Circular Supercoiling Biosensors for MicroRNA detection R43HG008709

Sponsor: NIH/SBIR Principal Investigator: Celedon, A Role: Co-I; Effort=1.6%

The main goal of this project is to develop novel tools to identify microRNAs from cancer cells.

04/01/16–03/31/17 Fourier transform Orbitrap Fusion Lumos tribrid mass spectrometer with ETD S10OD021844 Sponsor: National Institute of Health Principal Investigator: Akhilesh Pandey Role: Co-I The main goal of this project is to obtain a new Orbitrap Fusion Lumos tribrid mass

spectrometer with ETD to Johns Hopkins 09/01/15–08/31/17 Post-transcriptional Gene Regulation by Cytoplasmic Poly(ADP-ribose) polymerases

(Diversity Supplement) R01GM104135-S1 Sponsor: NIH/NIGMS

This is a supplement for training a graduate student from under-represented minority. 04/15/17–04/14/18 A nanoLC-orbitrap tribrid instrument for comprehensive proteomics analyses 1S10OD021502-01A1 Sponsor: NIH Principal Investigator: Ong, Shao-En (University of Washington) Role: Co-I The main goal of this project is to obtain a Thermo Orbitrap Fusion Lumos and an

Ultimate 3000 RSLCnano UHPLC instrument at my collaborator’s lab. 05/15/17–04/30/18 Research and screening platform for Alzheimer’s and other chronic diseases R43AG056208 Sponsor: NIH/NIA Principal Investigator: Celedon, A Role: Consultant Effort: 2% The main goal of this project is to develop novel tools to detect biomarkers of Alzheimer’s

and other chronic diseases. My role is as a consultant on microRNA technologies.

Anthony K. L. Leung’s CV Page 15 of 19

INTRAMURAL Funding Research Intramural Funding (i) Current 07/01/17–01/31/19 Uncovering Poly(ADP-ribose) Biology in Non-membranous Structures Discovery Award Sponsor: Johns Hopkins University Principal Investigators: Leung, AKL (contact PI), Myong, S and Greenberg, M. Effort: 10% The main goal of this project is to develop an interdisciplinary team to develop novel

chemical and biophysical tools to investigate poly(ADP-ribose) biology 07/01/17–06/30/19 Robust Identification of ADP-ribosylation Sites in Tissue Samples: Identifying

Biomarkers of PARP inhibitor responders The Technology Development Accelerator Fund Seed Grant Sponsor: The Johns Hopkins University Bloomberg School Of Public Health Effort: 5% The main goal of this project is to develop proteomics approach to analyze ADP-

ribosylation sites in tissue samples. 01/01/18–12/31/18 Understanding how virus virulence is regulated by the ADP-ribosylhydrolase activity

of the macrodomain—a potential drug target Fisher Center Discovery Program Sponsor: The Sherrilyn and Ken Fisher Center for Environmental Infectious Diseases, Johns Hopkins University School of Medicine Effort: 5%

The main goal of this project is to investigate the role of macrodomain in human and mosquito cells.

(ii) Previous Grants/Contracts 01/01/2013–12/31/2013 Designing inhibitors against PARP-12 – a member of a novel poly(ADP-ribose)

polymerase family subclass Translational Research Award

Sponsor: Johns Hopkins Medical Institute Brain Science Institute Effort: 5% The main goal of this project is to develop inhibitor against mono(ADP-ribosyl)transferase

PARP-12 05/01/2013–04/30/2014 Circulating RNAs as Trans-generational Gene Regulators and Disease Biomarkers Faculty Innovation Award Sponsor: Johns Hopkins Bloomberg School of Public Health Effort: 5%

The main goal of this project is to profile circulating RNAs in fetal cord blood samples 09/1/2013–08/31/2014 Understanding the mechanism of poly(ADP-ribosyl)ation-regulated ubiquitination Pilot grant Sponsor: Johns Hopkins Tech Center for Networks and Pathways derived from U54–

GM103520 Effort: 5%

The main goal of this project is to identify the ubiquitinated proteome and quantitatively compare the proteomes in various conditions when poly(ADP-ribose) level is altered.

07/01/14–06/30/15 Novel Proteomics Approaches to Identify Breast Cancer Patients Responsive to

PARP inhibitors Pilot grant

Anthony K. L. Leung’s CV Page 16 of 19

Sponsor: Safeway Research Foundation Effort: 5%

The main goal of this project is to use a novel proteomics technique to identify biomarker for PARP inhibitors in breast cancers.

01/15/16–01/14/17 The role of nsP3 in neurovirulence of chikungunya virus Pilot grant Sponsor: The Sherrilyn and Ken Fisher Center for Environmental Infectious Diseases Discovery Program, Johns Hopkins University Principal Investigator: Griffin, DE Role: Co-I The main goal of this project is to identify the role of nsP3 in neurovirulence of

chikungunya virus. 07/01/15–12/31/16 A Novel Proteomics Approach for Characterizing Macrodomain—A Potential

Antiviral Drug Target Catalyst Award Sponsor: Johns Hopkins University Effort: 5% The main goal of this project is to investigate the role of macrodomain in Sindbis Virus 04/01/15–03/31/17 Using PARylation Signature to Identify Prostate Cancer sensitive to PARP inhibitor Pilot grant

Sponsor: Patrick C. Walsh Cancer Research Fund Effort: 5%

The main goal of this project is to use a novel proteomics technique to identify biomarker for PARP inhibitors in prostate cancers.

04/01/15–03/31/17 Nucleolus: a novel paradigm for PARP1/2 functions and clinical application

Pilot grant Sponsor: Johns Hopkins–Allegheny Health Network Cancer Institute Principal Investigator: Laiho, M

Role: Co-Principal Investigator; Effort: 5% The main goal of this project is to use a novel proteomics technique to identify PARylation substrates in the nucleolus.

ACADEMIC SERVICE Institutional Administrative Appointments Johns Hopkins University 2015 Member, Core-In-A-Box Workgroup – a taskforce to reform the core facilities across the University 2016 Reviewer, Provost’s Undergraduate Research Award (PURA) 2017– Member, Curriculum Committee, Chemistry–Biology Interface 2017 Reviewer, Hopkins–Allegheny Health Network Cancer Research Fund 2018 Reviewer, Johns Hopkins Catalyst Award Johns Hopkins Bloomberg School of Public Health 2016 Member, Focus group in reforming PhD education 2017– Member, Bloomberg American Health Initiative (BAHI) Investigator Search Committee (Obesity and

Food Systems) 2016–2018 Elected Faculty Senator (subcommittee member in Discretionary Fund workgroup in 2017–18) Department of Biochemistry and Molecular Biology 2012, 2016 Speaker, University of Maryland Baltimore County–Meyerhoff Bridge Day to promote science amongst

undergraduate minority students

Anthony K. L. Leung’s CV Page 17 of 19

2014– Chair, Online Communications Committee. Portfolio includes department website, PhD program website (www.jhu-bmb-phd.org) and e-newsletter.

2015– Member, Curriculum Revision Committee 2015, 2018 Ad hoc Member, Sharon Krag Achievement Award Committee 2016– Member, Curriculum Committee 2016– Ad hoc Member, PhD recruitment Committee 2016 Speaker, BMB PhD program recruitment 2017 Ad hoc Member, Sharon Krag, John Scocca and Larry Grossman Achievement Award Committee 2017– Chair, Cecile M. Pickart Memorial Lecture Nomination Committee 2018 Ad hoc Member, PhD admission Committee Johns Hopkins University 2015 Member, Core-In-A-Box Workgroup – a taskforce to reform the core facilities across the University

2016 Reviewer, Provost’s Undergraduate Research Award (PURA)

2017– Member, Curriculum Committee, Chemistry–Biology Interface

2017 Reviewer, Hopkins–Allegheny Health Network Cancer Research Fund

INVITED PRESENTATION Johns Hopkins University/Regional 1. Apr 2010 Invited Speaker, Johns Hopkins University Bloomberg School of Public Health, Department of

Biochemistry and Molecular Biology, Baltimore, USA 2. May 2011 Invited Speaker, Johns Hopkins University, Breast Cancer Program Translational Research Seminar Series,

Baltimore, Maryland, USA 3. Sep 2011 Invited Speaker, Johns Hopkins University School of Medicine, Department of Cell Biology, Baltimore,

USA 4. Oct 2011 Invited Speaker, Johns Hopkins University School of Medicine, Institute of Genetic Medicine, Baltimore,

USA 5. Apr 2012 Invited Speaker, Johns Hopkins University School of Medicine, Brain Science Institute, Baltimore, USA 6. Apr 2012 Invited Speaker, Johns Hopkins University School of Medicine, Department of Biological Chemistry,

Baltimore, USA 7. Jan 2013 Invited Speaker, Carnegie Institute for Science, Baltimore, Maryland, USA 8. Mar 2013 Invited Speaker, Johns Hopkins University School of Medicine, Department of Molecular and Comparative

Pathobiology, Baltimore, USA 9. Jun 2013 Invited Speaker, Johns Hopkins University School of Medicine, Tech Center for Networks and Pathways,

Baltimore, USA 10. Mar 2014 Invited Speaker, Baltimore Area Repair Symposium, co-organized by Johns Hopkins University, University

of Maryland and National Institute of Aging. 11. Mar 2014 Invited Speaker, Johns Hopkins University, Breast Cancer Program Translational Research Seminar Series,

Baltimore, Maryland, USA 12. Mar 2014 Student invited seminar, Baltimore Life Scientists Association, Baltimore, Maryland, USA 13. May 2015 Invited Speaker, 8th Annual Safeway Breast Cancer Research Retreat, Baltimore, Maryland, USA 14. Jul 2015 Fellow invited seminar, National Institute of Aging, Bayview Campus, Maryland, USA 15. Sep 2015 Invited Speaker, Johns Hopkins Breast Cancer Survivorship Program, Maryland USA 16. Sep 2015 Invited Speaker, Johns Hopkins University, Bloomberg School of Public Health, Department of

Biochemistry and Molecular Biology Maryland, USA 17. Nov 2015 Invited Speaker, National Cancer Institute, Bethesda, Maryland, USA 18. Nov 2015 Invited Speaker, Johns Hopkins University School of Medicine, Department of Radiation Oncology and

Molecular Radiation Sciences, Baltimore, USA 19. Feb 2016 Invited Speaker, Johns Hopkins University, Department of Biochemistry and Molecular Biology, PhD

recruitment Program, Baltimore Maryland, USA 20. Mar 2016 Invited Speaker, Johns Hopkins University, Chemistry–Biology Interface Forum, Baltimore, Maryland, USA

Anthony K. L. Leung’s CV Page 18 of 19

21. Jun 2016 Invited Speaker, National Cancer Institute, Center for Cancer Research RNA seminar Series, Frederick, Maryland, USA

22. Sep 2016 Co-organizer, Session leader and Speaker, Johns Hopkins University, Biology, Chemistry and Physics of Non-membranous Granules at Johns Hopkins, Baltimore, Maryland, USA

23. Oct 2016 Invited Discussant, Nutritional Genomics and Public Health Lectures, Johns Hopkins School of Public Health, Baltimore, Maryland, USA

24. Oct 2016 Invited Speaker, 11th Annual Johns Hopkins Prostate Research Day, Baltimore, Maryland, USA 25. Dec 2016 Invited Speaker, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Breast Cancer

Tumor Board, Baltimore, Maryland, USA 26. Feb 2017 Invited Speaker, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Translational

Research Conference, Baltimore, Maryland, USA 27. May 2017 Invited Speaker, Breast Cancer Tumor Board Meeting, Sidney Kimmel Cancer Center at Johns

Hopkins, Baltimore, Maryland, USA 28. May 2017 Invited Speaker, Breast Cancer Survivors' group, Johns Hopkins Hospital, Baltimore, Maryland,

USA 29. Sep 2017 Student Invited Speaker, Johns Hopkins Chemistry–Biology Interface Graduate Program 13th Annual

Retreat, Baltimore, USA 30. Dec 2017 Invited Speaker, University of Maryland RNA club, Baltimore, Maryland, USA 31. May 2018 Invited Speaker, Biology of Healthy Aging lecture series, Baltimore, Maryland, USA 32. Jun 2018 Invited Speaker, Oophest, Johns Hopkins Pathology Department Division of Gynecologic Pathology,

Baltimore, Maryland, USA 33. Dec 2018 Invited Speaker, Breast and Ovarian Cancer Program Seminar, Sidney Kimmel Cancer Center at Johns

Hopkins, Baltimore, Maryland, USA (scheduled)

National 34. Mar 2008 Invited Speaker, 1st HHMI RNA granules Meeting, Chevy Chase, Maryland, USA 35. May 2008 Invited Speaker, Society of Biological Psychiatry, 63rd Annual Meeting, “MicroRNAs: Emerging Players of

Brain Function and Mental Illness” symposium, Washington D.C., USA 36. May 2009 Invited Speaker, The New York Academy of Sciences, “RNA in Stress Response and Longevity Control”

symposium, New York, USA 37. Jan 2010 Invited Speaker, New York University, Center for Genomics & Systems Biology, New York, USA 38. Feb 2010 Invited Speaker, Duke University Medical Center, Department of Biochemistry, Durham, North Carolina,

USA 39. Feb 2010 Invited Speaker, Cleveland Clinic, Lerner Research Institute, Cleveland, Ohio, USA 40. Feb 2010 Invited Speaker, London Research Institute, Cancer Research UK, London, UK 41. Mar 2010 Invited Speaker, Buck Institute, San Francisco, California, USA 42. Apr 2010 Invited Speaker, Columbia University Medical Center, Department of Biochemistry and Molecular

Biophysics, New York, USA 43. Apr 2010 Invited Speaker, Rockefeller University, New York, USA 44. Apr 2010 Invited Speaker, Harvard University School of Public Health, Department of Genetics and Complex

Diseases, Boston, Massachusetts, USA 45. Apr 2011 Invited Speaker, New England RNA Data Club, Harvard Medical School, Boston, Massachusetts, USA 46. Apr 2011 Invited Speaker, 5th Annual RNAi and microRNA World Congress, Boston, Massachusetts, USA 47. Nov 2011 Invited Speaker, U.S. Air Force Office of Scientific Research, San Antonio, Texas USA 48. Mar 2012 Invited Speaker, University of Washington, Department of Pharmacology, Seattle, Washington, USA 49. Apr 2012 Invited Speaker, Genomics Research 2012, Boston, Massachusetts, USA 50. May 2012 Invited Speaker, National Institutes of Health, Bethesda, Maryland, USA 51. May 2012 Invited Speaker, University of Maryland, College Park, Maryland, USA 52. May 2013 Keynote Speaker, Genomics Research 2013, Boston, Massachusetts, USA 53. April 2014 Invited Speaker, Inaugural Cold Spring Harbor Laboratory Meeting, The PARP Family & Friends: Gene

Regulation and Beyond, Cold Spring Harbor, New York, USA 54. May 2015 Invited Speaker, RNAi/MicroRNAs-Boston-2015 Meeting, Boston, Massachusetts, USA 55. Jun 2015 Invited Speaker, Penn State College of Medicine, Hershey, Pennsylvania, USA

Anthony K. L. Leung’s CV Page 19 of 19

56. Apr 2016 Invited Speaker, Cold Spring Harbor Laboratory Meeting, The PARP Family & ADP-ribosylation, Cold Spring Harbor, New York, USA

57. Sep 2016 Invited Speaker, University of Pennsylvania, Department of Biochemistry and Biophysics, Philadelphia, Pennsylvania, USA

58. Nov 2016 Student invited seminar, Catholic University of America, Washington D.C., USA 59. Jan 2017 Invited Speaker, Ribon Therapeutics, Inc., Lexington, Massachusetts, USA 60. Jun 2017 Invited Speaker, Annual Society of Virology 2017, Madison, Wisconsin, USA 61. Jul 2017 Session Chair and Invited Speaker, FASEB Meeting “NAD+ Metabolism and Signaling 2017”, New

Orleans, Louisiana, USA 62. Apr 2018 Invited Speaker, The University of Alabama at Birmingham, Department of Chemistry 63. Apr 2018 Invited Speaker, Cold Spring Harbor Laboratory Meeting, The PARP Family & ADP-ribosylation, Cold

Spring Harbor, New York, USA 64. Apr 2018 Invited Speaker, University of South Carolina, College of Pharmacy, Columbia, South Carolina, USA 65. May 2018 Invited Speaker, 11th Annual Frontiers at the Chemistry & Biology Interface Symposium, Philadelphia,

Pennsylvania, USA 66. Jul 2018 Invited Speaker, Ribon Therapeutics, Inc., Lexington, Massachusetts, USA (scheduled) 67. Mar 2019 Invited Speaker, Baylor College of Medicine, Department of Molecular Physiology and Biophysics, Houston,

Texas, USA (scheduled)

International 68. Jun 2011 Invited Speaker, Hong Kong University of Science and Technology, Division of Life Science, Hong Kong,

China 69. Jun 2011 Invited Speaker, Academia Sinica, Genomics Research Center, Taipei, Taiwan 70. Jun 2011 Plenary Speaker, RNA meeting 2011, Kyoto, Japan 71. Jun 2011 Invited Speaker, Osaka University, Immunology Frontier Research Center, Osaka, Japan 72. Sep 2013 Invited Speaker, PARP2013, 50th anniversary meeting for the discovery of poly(ADP-ribose), Quebec,

Canada. 73. June 2014 Invited Speaker, 1st symposium on stress-associated RNA granules, Halifax, Nova Scotia, Canada. 74. Aug 2015 Session Chair and Invited Speaker, FASEB Meeting "NAD+ Metabolism and Signalling 2015”,

Germany 75. May 2017 Invited Speaker, University of Oxford, Department of Biochemistry, Oxford, United Kingdom 76. May 2017 Session Chair and Invited Speaker, PARP2017, 20th International Conference on ADP-ribosylation,

Budapest, Hungary. 77. Oct 2018 Invited Speaker, 3rd International Symposium in Epigenetic Mechanisms and Human Health, Shenzhen,

China (scheduled) 78. Nov 2018 Invited Speaker, Université de Montréal, Département de biochimie, Montréal, Quebec, Canada (scheduled)