Strg_torey Foundation Hanif 27 8

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ITSFINDONESIA TORAY SCIENCE FOUNDATION

SCIENCE AND TECHNOLOGY RESEARCH GRANT

2012

GUIDELINES FOR APPLICATION FOR THE ITSF SCIENCE & TECHNOLOGY RESEARCH GRANT

1. Qualifications of Candidatesa. A research team consists of minimum of 2 (two) researchers.b. Energetic and creative young Indonesian researchers, in a team both chief and co-

researchers aged below 45 on January 1, 2013.c. Residing in Indonesia, engaged in research and/or science based innovation in Science and

Technology, excluding mathematics, clinical medicine and social sciences.d. Principal investigator is eligible only to submit one proposal for the same fiscal year. STRG

can only be granted twice. For those who have received previously STRG, current submittedproposal should be a continuation of previous research.

e. The proposed work should be carried out at a research facility in Indonesia and the result ofwhich may contribute to the advancement of Science and Technology.

2. Research GrantSTRG is provided annually for the duration of one year.ITSF will provide a total amount of approximately Rp 700 millions for 2012 calendar year ingrants of up to Rp 50 millions per approved proposal. This research grant is partly supported byToray Science Foundation Japan. Any ensuing intellectual property rights resulting from thisresearch will not be claimed by ITSF.

3. RecommendationThe proposal should be recommended by a representative of a scientific research institution.For an institution of higher learning, this should be the chairman of the Research Institute or theDean of Faculty. For a non-university research center, this should be its director.

4. Application Procedure

The applicant should complete the application form supplied by ITSF and send 5(five)copiesof the completed form, which are nicely bound with a transparent plastic covertoITSF at the mailing address stated herein.

The proposal is only submitted to ITSF. It is possible that the proposal is a part of a largerresearch project. It is, however, to be understood that the part of research eventually funded byITSF should be reported separately.

5. DeadlineThe completed forms should reach the foundation office in Jakarta on or before 31 August2012.

6. Selection MechanismThe selection of the Grantees will be done in 3 stages:

1

st

Stage : Shortlisting of applicants by the Selection Committee through a review of thecompleted application forms. Where necessary, additional reviewers may beinvited.

2nd

Stage : Interview of shortlisted applicants leading to a decision on selected grantees.The interview will be carried out only if necessary.

3rd

Stage : Approval of selected grantees by ITSF

The decision of ITSF is final and no correspondence regarding the decision will be entertained.


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7. Selection Commit teeThe Selection Committee members are:Dr. Suwarto Martosudirjo, APU (Chairman)Dr. Debbie S Retnoningrum (Member)Prof. Dr. Ir. Dedi Fardiaz, M. Sc (Member)

8. Science and Technology Research Grant wi ll be presented in ceremony in February 2013

9. Presentation of Research Results in a SeminarThe chief researcher who receive STRG is required to present his/her research results in theITSF Science and Technology Seminar tentatively scheduled in February 2014, which is heldone day prior to the ceremony of that particular year. A presentation paper should besubmitted at the latest two weeks before the seminar.

10. Disbursement ProcedureThe grant is to be disbursed to the grantees according to the agreed procedure within ITSF.

Notes:a) The research grant may be used in any way, as long as it helps in the achievement of the

purpose of the research. However, if the recipient desires to use the grant for an additionalresearch purpose within the agreed upon research objective, prior written consent from ITSFmust be received.

b) The grant should be fully spent according to the approved schedule.c) Each STRGs recipient should submit 1 (one) softcopy,3 (three)copies of a final report(hard

copies), including a three to fi ve-page extended abstract,at the latest one monthafter theseminar.

d) The Final Report to some extent should be able to represent the full report & article format,should contain:

Background of the research

Nature of the research problem

Methods

Analysis of data and information obtained

Conclusion

Contribution to the existing body of knowledgee) Original photographs where applicable should be provided and included in the report.

In case of data processing, simulation etc, a listing of developed computer programs as well asthe use of readily available programs should be reported.f) For any additional clarification, please send e-mail, phone or fax to ITSF at the address/numbers

stated below.g) This grant is not taxable on the part of the recipient.h) The applicant will make sure that the proposal is not submitted to other sources of funding for

consideration of research grant.

Mailing Address/Enquiries to:INDONESIA TORAY SCIENCE FOUNDATION

Summitmas II - 3rd

FloorJl.Jenderal Sudirman Kav. 61-62

Jakarta 12190 - Indonesia

Tel: (021) 5220785, 2526841Fax: (021) 5202041E-mail: i [email protected]

Homepage: http://www.itsf.or.id


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ITSF

INDONESIA TORAY SCIENCE FOUNDATION

APPLICATION FORMSCIENCE AND TECHNOLOGY RESEARCH GRANT

DATE :

TO : INDONESIA TORAY SCIENCE FOUNDATIONSummitmas II 3

rdFloor

Jl. Jenderal Sudirman Kav. 61-62Jakarta Selatan 12190

Tel : (021) 5220785, 2526841Fax : (021) 5202041Homepage : http://www.itsf.or.idEmail : [email protected]

(This form should be completed, typed or printed in English. Use a separate form for each nomination. You need not use thisform if you can reproduced and complete it in the same format using photocopier or a word processor).

Please read the guidelines carefull y before you f ill in the form.Failure in submitting necessary attachments will result in th e nomination not beingconsidered.

A recent photograph of the candidate should be attached.The proposals with all necessary attachments should be nicely bound with transparent plastic cover,send 5 (five) copiesto ITSF at the mailing address stated herein.

Particular of Recommending Institution

Name of Institution :The Agency for the Assessment and Application of Technology (BPPT)

Center : Center for the Industrial Processed of Technology (PTIP)

Address : Gedung Teknologi 2 Puspiptek Serpong Tangerang

Province : Banten Post Code : 15314

Telephone : (021) 75875940 Fax : (021) 75875938

Email address : [email protected]

Name & Title of Representative : Dr. Dra. Nadirah M.ScDirector

(Chairman/Dean/Director)**) choose the appropriate one

RESEARCH GRANT

2012 19th


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I recommend this candidate for the ITSF Science & Technology Research Grant onthe following basis and supporting reasons:

- The candidates are doctoral students in chemical engineering and need to increase

research in their field to find the novelty.- The research's topic is antimicrobial assays of secondary metabolite from selected

microbes in order to find new antibiotics compound.

From the advantages of the researches for biotechnology and chemical engineering

development, and for the wide applications and also have potential to be patented, we

recommend the candidate's proposal for the ITSF and Technology Research program.

Director of PTIP ,

August, 30 2012

Dr. Dra. Nadirah, M.Sc

NIP. 195712151985032001 Date

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Particular of Chief Researcher

Name & Title : Hanif Yuliani, SSi. MT.

Place & Date of Birth : Tulungagung, July 18, 1981

Age : 31

Sex : Male Female

Name of Institution : The Agency for the Assessment and application of Technology (BPPT)

Position of Candidate : Research Engineer

Address of Institution : Gedung Teknologi 2 Puspiptek Serpong Tangerang

Province : Banten Post Code : 15314

Telephone : (021) 75875940 Fax : (021) 75875938

Home address : Jalan Margonda No. 507, Depok

Province : Jawa Barat Post Code : 16424

Telephone number : - Fax No. : -

HP number : 081234564807 Email : [email protected]

Particular of Co-Researchers

Name : Imelda Krisanta Enda Savitri

Date of Birth : October, 30 1966

Age : 45

Sex : Male Female

Name of Institution : Department of Chemical Eng. UI

Address of Institution : Jl. Prof.Somantri Brojonegoro, Kampus UI, Beji, Depok

Province : west Java Post Code : 16424

Telephone : (021) 7863516 Fax (021)7863515

HP number : 08121854044 Email :[email protected]

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Title of Research Project:

Antimicrobial potential o f a lipopeptide biosurfactant derived f rom a Bacillus subtilisC19

Purpose o f Research

Lipopeptides represent a class of microbial surfactants with increasing scientific, therapeuticand biotechnological interests. The genus Bacillus is a producer of these active compounds,and among of them Bacillus subtilis produces surfactin, the most potent biosurfactantknown. These compounds can act as antibiotics, antivirals, antitumorals, immunomodulatorsand enzyme inhibitors. In this work, the antimicrobial activity of biosurfactants obtains bycultivation of Bacillus subtiliswas investigated.

Summary of research (in ord inary language) max 100 words- Key technology : Antimicrobial lipopedtide- Purpose : Isolate biological active fraction of biosurfactant for antimicrobial potential- Result : New antimicrobial compound.- Impact : increase variety of antibiotics compound with higher activity.

Desired amount of research grant: Rp 49,934,000.00

Schedule for usage of grant:

Commencement date Completion date

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1. Outline of Research Plan

IntroductionMicrobial surfactant or biosurfactant are surface-active amphipathic moleculesproduced by a number of microorganisms. They occur in nature as a diverse groupof molecules comparing of glycolipids, lipopeptides and lipoproteins, fatty acid,neutral lipids, phospholipids, polymeric and particulate biosurfactant (Desai andBanat, 1997). Beside their regular roles in enhanced oil recovery, bioremediationand industrial emulsification, in recent years, microbial surfactants have been foundto possess several properties of therapeutic and biomedical importance, e.g.antibacterial, antifugal and antiviral properties. They also have antiadhesive actionagainst several pathogenic micro-organisms (Cameotra and Makkar, 2004).

Among the genus Bacillus, Bacillus subtilisproduces a broad spectrum of bioactivelipopeptides which have a great potential for biotechnological and biopharmaceuticalapplications. Other antimicrobial lipopeptides include fengicyn, iturin, bacillomycinsand mycosubtilins produced by B. subtilis(Vater et al,2002). Lichenysin (Yokimov etal, 1995) and and pimilacidin (Naruse et al, 1990) produced by Bacillus licheniformisand Bacillus pumilus, respectively are the other antimicrobial lipopeptides. Otherbiosurfactant that are reported of having antimicrobial actions are rhamnolipids

produced by Pseudomonas aeruginosa (Abalos et al, 2001) and soporolipidsproduced by Candida bombicola(Kim et al, 2002). Mannosylerythritol lipids producedby Candida antarctica strain exhibit antimicrobial action against gram-positivebacteria (Kitamoto et al, 1993).Isolation and purification of a lipopeptide biosurfactant from a marine Bacillus subtilisC19 and its antimicrobial potentials against pathogenic and semi-pathogenicbacterial strains along with a few multidrug-resistant (MDR) clinical isolate arepropose of this research.

Materials and MethodsMicro-organism, production medium and culture conditionsMicroorganism used in this research was isolated from marine Indonesian, collectionof research center for biotechnology, Indonesian Institute of Science. It was later

characterized as Bacillus subtilis. A sucrose minerals salts (SMS) medium will beused for the production of biosurfactant. The Sucrose salt medium productionmedium which is utilized for biosurfactant production has the following composition:Sucrose (2%), NH4NO3(0.3%), K2HPO4(0.22%), KH2PO4(0.014%), NaCl (0.001%),MgSO4 (0.06%), CaCl2 (0.004%), FeSO4 (0.002%) and 0.5 ml/L of trace elementsolution containing (L-1): 2.32 g of ZnSO4.7H2O, 1.78 g of MnSO4.4H2O, 0.56 g ofH3BO3, 1.0 g of CuSO4.5H2O, 0.39 g of NaMoO4.2H2O, 0.42 g of CoCl2.6H2O, 1.0 gof EDTA, 0.0004 g of NiCl2.6H2O and 0.66 g of KI. Nutrients broth will be used forpreparation of primary inoculum. After overnight growth, the inoculum will betransferred to 100 ml of sterile SMS production medium in 500 ml Erlenmeyer flasksand incubated at 37oC with shaking at 120 rpm for 96 h.

Isolation of Surface-active molecules

The biosurfactant will be precipitated from cell-free broth of the culture of 96 h byadjusting the broth pH to 2.0 using 6 N HCl and keeping it at 4 oC overnight.Precipitated material will be collected by centrifugation, 11.000xg for 20 min. thecrude biosurfactant will be lyophilized and be weighed for quantification. For theextraction of biosurfactant compound, 50 mL of chloroform-methanol (2:1 v/v) will beadded to 500 mg of the dry product and incubated in rotatory shaker at 250 rpm,30oC, for 15 min. the mixture will be filtrated using 0.45 mm Millipore membrane. The


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filtrate (50 mL) will be used for thin layer chromatography (TLC) analysis andantimicrobial activity tests.

Purification of biosurfactant by high-performance liquid chromatographyHigh-performance liquid chromatography (HPLC) will be used to resolve the differentbiosurfactant fraction in order to check their individual antimicrobial activities.Reverse phase (RP-HPLC) will be performed with reverse phase C18 column. Theelution will be done using a linear gradient of 5-95% of acetonitrile containing 0.1%(v/v) trifluoroacetic acid (TFA) and water for 40 min at flow rate of 0.4 ml/min. theabsorbance of eluent will be measured at 210 nm. The resolved biosurfactantfraction will be collected in a fraction collector for activity studies.

Thin layer chromatographyThe chemical characterization of the isolated compounds will be done by TLCanalysis. The components of the chloroform-methanol extract will be separated onsilica gel (Si 60 F254, 0.25 mm, Merck) using chloroform-methanol-water (65:25:4v/v/v) as a solvent system. Spots will be revealed by spraying with: a) distilled waterand heating at 110oC for 5 min, for detection of hydrophilic compounds; b) ninhydrin0.05% w/v (in methanol-water 1:1 v/v) and heating at 100oC for 4-5 min, for detectionof compound with free amino groups; and c) rhodamine B 0.25% w/v (in absoluteethanol) for detection of the presence of lipids under ultraviolet light. Surfactin(Sigma) will be used as a standard.The component of the chloroform-methanol extract will be submitted to acidhydrolysis in 6 N HCl at 105oC for 24 h for detection of free amino groups byninhydrin 0.05% w/v by TLC

Fourier transform in frared spectroscopyFourier transform infrared spectroscopy (FTIR) will be used to determine thefunctional groups and the chemical bonds present in the biologically active fraction ofthe biosurfactant and thus determine its chemical nature. The FTIR analysis will beperformed by using a Nexus-870 FT-IR spectrometer with sample (0.3-0.5 mg)dispersed in the pellets of KBr (80 mg). The spectrum of a standard lipopeptide

biosurfactant, surfactin, will also be obtained in parallel. The two spectra will becompared in order to confirm the chemical nature of active biosurfactant fraction.

Antimicrobial act ivi tyThe antimicrobial activity of the biosurfactant will be evaluated using the agardiffusion method proposed by Bauer et al(1966). In order to produce an appropriateinoculum an overnight culture (grown at 37oC) of bacteria in Mueller-Hinton broth willbe standardized to an optical equivalent to 0.5 on the McFarland scale (108CFU/mL). the resulting suspension will be diluted to yield a cellular concentration of107 CFU/mL. Two mL of standardized suspensions of the microorganisms will bedeposited in Petri dishes (diameter 90 mm) and 18 mL of Mueller-Hinton agar at45oC will be added. Aliquots of 20 mL of filtrate will be applied to paper disks (6mmin diameter, Whatman No 1), which resulted in disks containing 200 g of product.

After evaporation of the loading solvent, each disk will be placed at the center of thePetri dishes containing previously inoculated Mueller-Hinton medium and will beincubated at 37oC for 24 h. at the end of incubation time, the diameter of microbialgrowth inhibition halo will be measured.

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2. Proposed Use of the Research Grant

Materials unit Unit Price(IDR)

Total Price(IDR)

Sucrose, 500g 1 630,000.00 630,000.00

K2HPO4, 500 g 1 1,200,000.00 1,200,000.00

KH2PO4, 500 g 1 1,400,000.00 1,400,000.00

NaCl, 500 g 1 320,000.00 320,000.00MgSO4.7H2O, 500 g 1 1,440,000.00 1,440,000.00

CaCl2.2H2O, 500 g 1 1,530,000.00 1,530,000.00

FeSO4, 200 g 1 500,000.00 500,000.00

ZnSO4.4H2O, 500 g 1 1,450,000.00 1,450,000.00

MnSO4.H2O, 500 g 1 1,430,000.00 1,430,000.00

H3BO3, 500 g 1 1,310,000.00 1,310,000.00

CuSO4.5H2O, 200 g 1 320,000.00 320,000.00

Na2MoO4.2H2O, 200 g 1 1,260,000.00 1,260,000.00

CoCl2.6H2O, 200 g 1 200,000.00 200,000.00

EDTA, 100 g 1 1,260,000.00 1,260,000.00

NiCl2.6H2O, 500 g 1 1,310,000.00 1,310,000.00

KI, 200 g 1 1,200,000.00 1,200,000.00

Methanol, 5L 2 1,227,000.00 2,454,000.00Chloroform, 5L 2 2,100,000.00 4,200,000.00

Acetonitrile, 5L 1 2,160,000.00 2,160,000.00

TFA, 1L 1 2,600,000.00 2,600,000.00

Nutrient agar,500 g 2 2,490,000.00 4,980,000.00

Nutrient broth, 500 g 2 1,170,000.00 2,340,000.00

Mueller-Hinton broth, 500 g 2 1,830,000,00 3,660,000.00

Whatman paper disks,1 pack 1 800,000.00 800,000.00

TLC plate 1 2,100,000.00 2,100,000.00

Aquadest 70 30,000.00 2,100,000.00

Tube 1.5 mL (1000 pcs) 1 1,350,000.00 1,350,000.00

Petri disks 60 28,000.00 1,680,000.00

Pseudomonas Aeruginosa 1 250,000.00 250,000.00

Escericia coli 1 250,000.00 250,000.00

Proteus Vulgaris 1 250,000.00 250,000.00Serratia marcescens 1 250,000.00 250,000.00

Alcaligenes faecalis 1 250,000.00 250,000.00

Acetobacter calcoaceticus 1 250,000.00 250,000.00

Klebsiella aerogenes 1 250,000.00 250,000.00

Enterococcus faecalis 1 250,000.00 250,000.00

Staphylococcus aureus 1 250,000.00 250,000.00

Micrococcus flavus 1 250,000.00 250,000.00

Bacillus pumilus 1 250,000.00 250,000.00

Total 49,934,000.00

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3. Please state briefly the past achievements of the chief researcher and the co-researcher(s) related to this project.

The Past achievement of researches related to this project was summarized in thegraph below:

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4. Please state whether the chief researcher and co-researchers submi t thispurposed this propose research to other financial received any grant/subsidyfor any research project from any foundations or ministr ies during the past 3years (2009/2012)

YES NO

If the answer is yes, please state the institution which provided the grant, the year(s)and the topic(s) of the grant(s)

*) Please tick the appropriate box

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5. Biodata of the Chief Researcher(s)

I. HANIF YULIANI

Education

Since 2010 Doctorate program in Universiy of Indonesia, Jakarta, Indonesia.

Title of dissertation:Biodegradation ability of Indonesian marine bacteria forPolyaromatic Hydrocarbons biodegradation.

Abstract

There are many industries which are produce large amount of poly-aromatic hydrocarbon(PAHs). Bioremediation of PAHs contaminated environment needs to be done because of thecarcinogenic and mutagenic characters of PAHs compound. Five isolates of marine bacterialisolated from Indonesian territorial that have high potential for PAHs (phenantrene andpyrene) degradation was further analyzed. Five strains, named M2292, M128, C318, C19,and C15, have been identified by using 16S rDNA sequence analysis. The result wereOchrobactrum oryzae(99% homology),Bacillus subtilis(99 % homology),Bacillus subtilis(99 % homology), Bacillus subtilis (100 % homology), and Bacillus pumilus (99%homology), respectively. The initial dioxygenase genes of the five PAH-degrading bacteriawere investigated, and revealed that all of the five isolate possessed the nidA and nahAcgeneencoding the initial dioxygenase required for pyrene and phenanthrene degradation.

2006 2008 Insti tute Teknologi 10 Nopember, Surabaya, Indonesia

Master Degree (S2) Process technology, Chemical Engineering

Title of thesis:Preparation and Characterization slow release fertilizer (SRF)

Abstract

One of effort to increase urea fertilizer effectiveness and efficiency could be done bymodifying the product into slow-release fertilizer (SRF) form. One of nature mineral whichcan be used as SRF matrix is natural zeolite. This material is chosen because it has beenfound a lot in Indonesia and has characteristic excellence to be used as SRF material matrix.These characteristic excellences are for example has a wide cavity, polar, a relatively smoothcavity size between 4-7 , and has ionic exchange capacity. In this research natural zeolitewas taken at around Malang, and urea raw material was the product from Petrokimia Gresikfactory.Zeolite raw material preparation and characterization which was used in this research wasdone in four treatment variations that are without activation, physic activation, acid activationand base activation. Physic activation was done by heating at temperature 250 C for about 3

hour and 500 C for about 4 hour. Acid activation was used H2SO4 0,2 N which continuedwith heating at temperature 250 C for about 3 hour and heating at temperature 500 C forabout 4 hour. Base activation was used NaOH 0,5 N which continued with heating attemperature 250 C for about 3 hour and heating at temperature 500 C for about 4 hour.

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Zeolite quality as SRF matrix was measured by analyzed the characteristic specific surfacearea, adsorption capacity, and the release profile of urea analysis. The specific surface areawas analyzed using gas sorption analyzer NOVA 1000, bonding among element of matrixcompiler analysis using FTIR (Fourier transform infrared spectroscopy), Zeolite adsorptioncapacity to urea analysis using spectrophotometer UV-Vis and urea desorption profileanalysis from zeolite matrix using spectrophotometer UV-Vis.Specific surface area of natural zeolite from Malang with 150 mesh particle size at withoutactivation, physic activation, acid activation and base activation treatments were respectively30.9636, 34.4960, 25.3959, 27.0741 m2/g. Chemical activation which continued withcalcinations at 500 C for about 4 hour giving the specific surface area 32.2064 m 2/g for acidactivation and 39.4962 m2/g for base activation.At 50 mesh particle size with 70 minute elution time was obtained natural zeolite Malangadsorption capacity in without activation, physic activation, acid activation, and baseactivation treatments were respectively 0.3141, 0.4667, 0.2055, 0.2584 gurea/gzeolite. The ureapercentage which desorpted from SRF at without activation,physic activation, acid activation,and base activation treatments were respectively 38.69, 32.59, 38.37 and 39.15 %. The urea-zeolite difference composition 1:1, 1:2, 1:3 didnt give significant differentiation at ureadesorption percentage for about 32.59, 32.19, and 31.81 %. The solid-solid mixing methodgiving desorption percentage 32.59%, while the solid-liquor mixing method giving 32.52 %.And at 50,100,150 mesh particle size of zeolite matrix, desorpted urea were 32.59, 12.78 and11.30%

2000-2004 Ai rlangga Uni versi ty , Surabaya Indonesia

Bachelor Degree (S1) Chemical Science

Title of thesis:Isolation and Characterization of Biosurfactant Bacillus subtilis 3KP

Abstract

Strain 3KP, isolated from Kali donan river Cilacap and identified as Bacillus subtilis,produced a lipopeptide surfactant when cultured on molassess. The biosurfactant, termed

biosurfactantBacillus subtilis

3KP, was purified and characterized.Physical characteristics from purified biosurfactant Bacillus subtilis 3KP decreased thesurface tension of water from 72 mN/m to 30 mN/m and achieves the critical micelleconcentration with as little as 2 g/l. this biosurfactant has high capability to emulsify dieselfuel and kerosin with very high stability. Temperature in range 25 65 0C, pH in range 4-8,and salt concentration in range 0-4 did not show any influence on surface tension.Chemical characterization of this biosurfactant was identified by HPLC, GC-MS, and Aminoacid analyzer. This identification indicated that biosurfactant Bacillus subtilis 3KP hasdifference structure with surfactin, commercially biosurfactant yielded by Bacillus subtilisstrain ATCC. So this biosurfactant potential to produce in big scale, sold, and patented.

Job experience

- Fireproofing Assessment Project in Belida Platform Conoco Philips Indonesia(COPI), 2010.

- Developing Standar Specific Equipment Inspection Guide (SEIG) in PertaminaRefinery Unit VI, Balongan Indramayu, 2009-2010

- Member of Mirror Committee (MC) TC 67 Oil & Gas, Indonesian NationalStandardization Bodi / Badan Standarisasi Nasional (BSN) since 2008.

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- Member of Floating Flexible Hose Design and Manufacturing in Centre forIndustrial Process Technology in The agency for study and application of technology(BPPT), 2008-2011.

- Failure analysis of exploded electric motor in Conoco Philips Indonesia (COPI),2010.

- Failure Analysis of a broken gas turbine in Muara Tawar Electric PowerGeneration, Bekasi, West Jawa, 2011

- Isolation and characterization of biosurfactant produced by Bacillus Subtilis byusing Molases substrate.

- Preparation and characterization of nature Zeolite as a matrix of slow releasefertilizer.

II. IMELDA KRISANTA ENDA SAVITRI

1.1 Nama Lengkap (dengan gelar) Ir. Imelda Krisanta Enda Savitri, Msi

1.2 Jabatan Fungsional Dosen

1.3 NIP/NIK/No. Identitas lainnya 196610301993032001

1.4 Tempat dan Tanggal Lahir Ambon, 30 Oktober 1966

1.5 Alamat Rumah Jl. Bacang No. 35A. RT/RW: 007/06.

Tanjungbarat. Jagakarsa. Jakarta Selatan.

1.6 Nomor Telepon/Faks -

1.7 Nomor HP 08121854044

1.8 Alamat Kantor Jl. Chr. Soplanit. Poka. Ambon

1.9

Nomor Telepon/Faks 0911-379859/ 0911-379196

1.10 Alamat e-mail [email protected]

1.11 Lulusan yang Telah Dihasilkan

1.12 Mata Kuliah yang diampu

II. RIWAYAT PENDIDIKAN

S-1 S-2 S-3Nama PT Universitas

Pattimura

Institut Pertanian

bogor

Bidang Ilmu Teknologi Hasil

Perikanan

Teknologi Pasca

Panen

Tahun Masuk-Lulus 1985 - 1991 1995 - 1999

Judul Tugas Akhir Perubanhan

kandungan squalen

minyak hati cucu

botol Stelophorus

molucencisakibat

proses winterisasi

Penentuan umur

simpan biskuit

wafer menggunakan

model Arrhenius

dan Labuza

NamaPembimbing/Promotor

Ir. J.M. Nanlohy,Ir. F. Pattipeilohy

Prof. Rizal Syarief,Dr. Poerwijatno, Dr.

Made Asatawan

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6. List of Publications

- Hanif Yuliani, 2004, Isolation and characterization of biosurfactant produced

by Bacillus Subtilis 3KP by using Molases substrate, Airlangga University,

Surabaya

- Hanif Yuliani, , Heri Hermansyah, H, 2011, Produksi, Isolasi, dan Aplikasi

Biosurfaktan untuk Bioremediasi Lingkungan Tercemar Hidrokarbon, Jurnal

Kelautan Nasional.

- Imelda Krisanta Enda Savitri, Pengaruh penambahan buylated hydroxytoluene

(BHT) terhadap perubahan sifat fisik dan kimia minyk kasar hati ikan cucut

(Centrophorus antromarginatus), 2007, Genesis. Jurnal ilmu-ilmu MIPA.

Unpatti. ISSN : 1412-5692, 6:1. 1-5

- Imelda Krisanta Enda Savitri, Muhamad Sahlan, Anondho Wijanarko, 2011,

Rapid and Efficient Purification Method of Phospholipase A2 from Acathaster

planci, Intr. J. Pharma and Bio science, 2: B-401-406

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Strg_torey Foundation Hanif 27 8