Sterilization of Armamentarium

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    Blend H. M. Balisany

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    *Sterilization*To completely remove all kinds of microbes

    (bacteria, mycobacteria, viruses, & fungi) by

    physical or chemical methods

    *Effective to kill bacterium spores

    *Sterilant: material or method used to remove or

    kill all microbes

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    *Disinfection* To reduce the number of pathogenic microorganisms to

    the point where they no longer cause diseases

    * Usually involves the removal ofvegetative or non-endospore forming pathogens

    * May use physical or chemical methods

    *Disinfectant

    *Antiseptic*Degerming

    *Sanitization

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    STERILIZATION

    Methods of Sterilization

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    Steps followed in dental offices for achievement ofsterilization are:

    1. Cleaning:* Manual Cleaning.* Automated Cleaning

    2. Corrosion Control:* Dry instruments

    *Application of rust inhibitors

    3. Packaging:* For protection against contamination after sterilization.

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    *4. Sterilization:Heat: Heat sterilization is most commonly used

    in dental settings; however, steam andchemical vapor sterilization units are alsoused.

    * High temperature methods include steam, dryheat and unsaturated chemical vapor.

    *While low temperature automated sterilizationinvolves ethylene oxide (ETO) and plasmasterilization.

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    *Of these methods the AUTOCLAVES is the most

    preferred method in a dental setup.

    *Autoclave is a self locking setup based on the

    efficacy of steam under pressure of121 degree

    Celsius for 15 minutes and 15 pounds pressure.

    *Another method is that of "Flash Sterilization"

    for unwrapped instruments using 132 degreesCelsius at 30 pounds pressure for 4 -7 minutes.

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    *Hot air ovens are electrical devices used in

    sterilization. It uses dry heat to sterilize

    articles.

    *No much pressure build up within the oven,

    safer to work with.

    *As they use dry heat instead of moist heat,

    some organisms like prions, may not be killedby them every time.

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    * The standard settings for a hot air oven are:

    *1.5 to 2 hours at 160C (320 OF)

    *6to 12 minutes at 190C (374 OF)

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    *Liquid Sterilants used for "cold sterilization"

    are intended only for the reprocessing of heat-

    sensitive instruments and devices.

    *If the critical or semi critical item is heat

    sensitive, it should be immersed in a liquid

    sterilants.

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    *Transparent objects to visible light absorb UV.

    *UV irradiation is routinely used to sterilize the

    interiors of biological safety cabinets betweenuses, but is ineffective in shaded areas,

    including areas under dirt.

    *It also damages some plastics, such as

    polystyrene foam if exposed for prolongedperiods of time.

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    5. Storage & Shelf Life

    Packs and containers with sterile items should be

    stored 20-25 cm off the floor, 45-50 cm from theceiling and 15-20 cm from an outside wall.

    6. Monitoring Sterilization Procedures

    Sterilization procedures can be monitored

    routinely using a combination of biological,chemical and mechanical indicators as

    parameters.

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    Methods of Disinfection

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    Thank You