SOF-552 CYSTOMETRY DATA ANALYSIS - Med Associates...Required Data Signal Traces The analysis algorithm uses the Bladder Pressure, Infused Volume and Scale traces. These signals, among

SOF-552 CYSTOMETRY DATA ANALYSIS USER’S MANUAL

SOF-552 User’s Manual DOC-125 Rev. 1.4 Copyright © 2009 All Rights Reserved Catamount Research and Development, Inc. 650 Industrial Park Rd. St. Albans, Vermont 05478 www.catamountresearch.com

CATAMOUNT R&D, INC. CYSTOMETRY DATA ANALYSIS

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TABLE OF CONTENTS

Chapter 1 .............................................................................................. 1 Introduction .....................................................................................................1

Void Contractions vs. Non-Void Contractions ......................................................1 Analysis Algorithm Inputs................................................................................2 Analysis Algorithm Overview............................................................................4 Analysis Results .............................................................................................5

Chapter 2 .............................................................................................. 8 Interface Reference ...........................................................................................8

Cursor ..........................................................................................................8 Delta............................................................................................................8 Pan/Zoom Menu & Toolbar ..............................................................................9 Set Extents Dialog........................................................................................ 10 Cursor Menu ............................................................................................... 11 Analysis Menu & Toolbar ............................................................................... 12 Analysis Algorithm Inputs Dialog .................................................................... 13 Analysis Results Tables ................................................................................. 15 Import ....................................................................................................... 15 Export ........................................................................................................ 15 Print .......................................................................................................... 17

Chapter 3 ............................................................................................ 19 Using Cystometry Analysis ................................................................................ 19

Import Data ................................................................................................ 19 Load Bladder Pressure, Infused Total, and Scale traces ..................................... 19 Zoom into Micturition Cycle ........................................................................... 19 Set Analysis Marks ....................................................................................... 19 Set Analysis Parameters ................................................................................ 21 Perform Analysis .......................................................................................... 22 Manage Analysis Results ............................................................................... 23


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CHAPTER 1 Introduction

Attention

The Cystometry Data Analysis software may only be installed on systems equipped with Windows XP® Operating System with Service Pack 2 or higher.

Cystometry Analysis analyzes data acquired using Catamount Research and Development, Inc. Cystometry data acquisition software (MED-CMG). Cystometry Analysis software imports the data files (*.txt) created by MED-CMG into a database (*.cmg), then performs analysis utilizing the Bladder Pressure, Infusion Pump, and Scale traces to identify “Void Contractions” and “Non-Void Contractions”.

Void Contractions vs. Non-Void Contractions

A void contraction is defined as an increase in bladder pressure, caused by a bladder contraction, which results in the voiding of urine. A non-void contraction is an increase in bladder pressure that does not result in voiding of urine. Subjects with urinary tract or prostate blockages usually demonstrate several non-void contractions between each productive void contraction. In the Cystometrogram (CMG) shown in Figure 1, multiple non-void contractions can be seen (red arrows) between the void events (blue arrows).

Figure 1 - Void and Non-Void Contractions


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Analysis Algorithm Inputs

The algorithm used to locate the void and non-void contractions, requires:

• Three data signal traces from MED-CMG; • Four user-defined analysis marks; • Seven user-defined analysis algorithm parameters.

Required Data Signal Traces

The analysis algorithm uses the Bladder Pressure, Infused Volume and Scale traces. These signals, among others, are imported from a .txt file. The MED-CMG software creates the .txt files.

Analysis Marks

Figure 2 – Analysis Marks

The user must place four “marks” on the traces. These marks denote the following:

• 0 - Previous Micturition Cycle Threshold Pressure: used as the starting point for calculating the Inter-Micturition Interval time, and the micturition cycle Infused Volume.

• 1 – Micturition Cycle Start: indicates the starting point for finding Non-Void Contractions, Minimum Bladder Pressure, Average Bladder Pressure, and Scale Peak.

• 2 – Threshold Pressure: the point prior to micturition where the bladder pressure slope changes from gradual to steep. This point is used as the ending point for finding Non-Void Contractions, Minimum Bladder Pressure, Average Bladder Pressure, Infused Volume, and Inter-Micturition Interval. Also used as the starting point for finding Maximum Bladder Pressure.

• 3 – Micturition Cycle End: marks the end point for finding the Maximum Bladder Pressure and Scale Peak.


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Analysis Algorithm Parameters

To set the analysis algorithm parameters, select Analysis | Inputs. The screen shown in Figure 3 appears.

Figure 3 - The Algorithm Inputs Dialog (Analysis menu, Inputs option)

The Analysis Algorithm Inputs screen shown in Figure 3 contains the following:

Bladder Pressure Peak Detection Parameters – For Non-Void Contractions

Min Peak Height – minimum amplitude for an increase in bladder pressure not associated with urine loss to be considered a contraction event (non-void contraction).

Max peak Width – the maximum duration of a non-void contraction. This parameter allows the user to filter out long (slow) rises in bladder pressure that are not intended to be counted as non-void contractions.

Min Peak Width – the minimum duration for a non-void contraction. This parameter allows the user filter out short (rapid) rises in bladder pressure that might result from grooming, or other artifacts.

First Drop Detection Parameters

Scale Threshold Delta – the minimum increase in the scale signal that must occur to be considered a void event.

Scale Trigger Window – the length of time the algorithm uses to search for a void event. The scale signal must increase by more than the Scale Threshold Delta value within the Scale Trigger Window to be considered a void.


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Scale Max Smoothing Parameters

Scale Max Variability – a percent variability considered acceptable in the scale signal for determining the void mass.

When a drop of urine lands on the scale, often the scale signal rises abruptly and then settles at a lower value. As the animal voids, the drops accumulate and the scale signal rises. When the last drop lands, the scale signal usually increases to some value and then settles a few milligrams lower. The scale smoothing parameters were designed to prevent that last “blip” on the scale from being counted as the void mass. The algorithm averages all the data (scale signal) during the Scale Max Window. If the maximum value is more than the Scale Max Variability higher than the average, the data point is ignored, and the program continues to search for a maximum until the maximum varies less than the Scale Max Variability % from the average in the Scale Max Window.

Bladder Pressure Min Smoothing Parameters

None, 3, 5, 7 – number of data points in averaging window used to smooth bladder pressure trace when finding the minimum bladder pressure between marks 1 and 2.

Defaults

Click the Defaults button to load the default settings. The default values that are included with the software are a good starting point for rat cystometry analysis, however these values may need to be adjusted based on the species studied and the rates of infusion. The default values can be changed at any time by filling the Save these values as defaults checkbox before clicking OK.

Disable Scale Checking

If the Disable Scale Checking option is selected, the scale trace will not be used during analysis. Any void contractions found with the Scale Checking disabled will have a Scale Max and Void Rat Index values of 0. The time of the contraction will be the point of peak bladder pressure between the Threshold Pressure (2) mark and Micturition End (3) mark.

Analysis Algorithm Overview

The analysis algorithm identifies a void contraction as the maximum pressure that occurs between the Threshold and Cycle End marks, assuming that a void event was detected. A void is considered to occur when they scale signal increases by more than the Scale Threshold Delta value within a time less than the Scale Trigger Window value. If the Disable scale checking option is selected on the Analysis Algorithm Inputs dialog, the void event is not required.

The Scale Threshold Delta value may need to be changed based on the species of animal tested, as rats will urinate a greater mass than mice. Manually analyze the Scale trace using the scale trace and delta tool to determine a value that is high enough to eliminate scale drift and other unwanted events, but low enough to capture the Void Contractions. The scale trace must increase more than the Scale Threshold Delta


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value, in a period of time shorter than the Scale Trigger Window. Again, these steps do not apply if the Disable scale checking option is selected.

Once the Scale Threshold Delta value has been exceeded within the Scale Trigger Window period, the maximum scale value from the trigger point to the Micturition End is stored as the scale max value. This scale max value is subject to rejection if it differs from the scale average in the following Scale Max Window by a percentage greater than the Scale Max Variability percentage.

Then the algorithm will search for Non-Void Contractions between the Cycle Start and Threshold marks. The Non-Void Contractions are increases in the Bladder Pressure trace that exceed the Min Peak Height value, and occur between the Min Peak Width and the Max Peak Width values.

Analysis Results

The Cystometry Analysis software is used to analyze micturition cycles (Cystometrogram or CMGs) acquired using MED-CMG software. Analysis results are stored in a database (.cmg) file and displayed in the results tables and on printed reports. These values are described in the following tables.

Table 1 – Void Contraction Values

Result Field Description Start Time

(Marker ID) End Time

(Marker ID) Units

Time

Time where the scale trace exceeds the Algorithm Inputs Scale Threshold Delta within the Scale Trigger Window. Indicates when the first drop of urine hits scale.

Micturition Start (1)

Micturition End (3)

seconds

Inter-Micturition

Interval

Elapsed time between consecutive threshold marks. A measure of the duration between successive voids.

Previous Threshold

Pressure (0)

Threshold Pressure (2)

seconds

Scale Max

Maximum scale value between micturition start and end, subject to smoothing parameters in Algorithm Inputs dialog. A measure of voided urine volume.

Result will be “0” if Disable scale checking is selected.


Micturition End (3)

grams

Void Rate Index

Change in scale over change in time. A measure of the urine flow rate. The scale max volume (weight of voided urine) is divided by the length of time for the void to be complete (as measured from the scale baseline to the scale max). See Figure 4.

Result will be “0” if Disable scale checking is selected.

Scale Peak Threshold exceeded

Scale Max grams /

sec


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(Marker ID) Units

Infused Volume

Volume of saline infused during the micturition cycle. A measure of bladder capacity.

Previous Threshold

Pressure (0)


µL

Threshold Bladder Pressure

Bladder pressure at mark 2. Threshold

Pressure (2) Threshold

Pressure (2) cmH2O or

mmHg

Minimum Bladder Pressure

Lowest bladder pressure during bladder filling. This value may NOT be a raw value, but a “smoothed” value to eliminate odd outliers. See Figure 5.



cmH2O or mmHg

Average Bladder Pressure

Average bladder pressure during bladder filling.



cmH2O or mmHg

Maximum Bladder Pressure

Highest bladder pressure associated with voiding.


Micturition End (3)

cmH2O or mmHg

Figure 4 illustrates the components of Void Rate Index. Void Rate Index is the change in scale over the change in time, from the first urine drop on the scale to the scale maximum. The first urine drop is found when the scale change exceeds the Scale Threshold Delta value within a time less than the Scale Trigger Window value.

Figure 4 – Void Rate Index


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Figure 5 illustrates an example of an artifact that resulted in a false minimum pressure (green signal). The green trace is a computed signal, constructed by averaging the raw values in a sliding window. The size of the sliding window is specified on the Algorithm Inputs dialog, Bladder Pressure Min Smoothing Parameters section. To use the raw values to compute the minimum bladder pressure, specify “None” on the Algorithm Inputs dialog. This smoothed trace is used only for finding the minimum bladder pressure in the Void Contraction results table. The smoothed bladder pressure signal will not be displayed in the graphs.

Figure 5 - Smoothed Bladder Pressure Trace

Table 2 – Non-Void Contraction Values



(Marker ID) Units

Time Time at peak bladder

pressure. Micturition Start (1)


seconds

Pressure Baseline

Bladder pressure before contraction.



cmH2O or mmHg

Maximum Pressure

Peak bladder pressure. Micturition Start (1)


cmH2O or mmHg

Pressure Amplitude

Difference between peak and baseline pressures.



cmH2O or mmHg


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CHAPTER 2 Interface Reference

Figure 6 – Delta and Main Cursors

Cursor

The main cursor is a pair of yellow intersecting perpendicular lines (refer to Figure 6). To move the cursor with the mouse, select the Cursor Control button on the Analysis Toolbar, or in the Pan/Zoom menu. To move the cursor left and right along the time axis, click and hold the left mouse button on the vertical component of the main cursor, and drag left or right. Release the cursor to drop at new time. To move the main cursor with the keyboard, use the left and right arrow keys. This will move the cursor one data point – 0.25 second. Use the Shift key in combination with the arrow key to move the main cursor in 5-second increments.

Delta

The Delta cursor is a pair of white intersecting perpendicular lines (refer to Figure 6). To activate the Delta cursor, click the checkbox next to the desired graph. The difference between the Delta cursor’s Y-axis value and the main cursor’s Y-axis value will be displayed left of the Delta checkbox.


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Pan/Zoom Menu & Toolbar

Figure 7 - Pan/Zoom Toolbar

Figure 8 - Pan/Zoom Menu

The following table describes the commands found in the Pan/Zoom menu and the Pan/Zoom toolbar. These functions affect the area of the data signals viewed in the graph windows.

Table 3 - Pan/Zoom Manu Functions

Function Keyboard Shortcut

Toolbar Icon

Action

Zoom In X Ctrl++

X-axis (Time) extents of all visible graphs changed to half current range, centered on the time at the main cursor.

Zoom In Y Ctrl+Shift+

+

The active graph’s Y-axis (data signal amplitude) extents changed to half current range, centered on the current y value.

Zoom In Both N/A Zoom in X and Zoom in Y simultaneously.

Zoom Out X Ctrl+- X-axis (Time) range doubled for all visible graphs, centered on the main cursor time.

Zoom Out Y Ctrl+Shift+- Y-axis of active graph is doubled, centered on main cursor’s Y value.

Zoom Out Both N/A Zoom Out X and Zoom Out Y simultaneously.

Zoom Out Full N/A View active graph’s entire data signal.


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Toolbar Icon

Action

Lasso Zoom Ctrl+L Mouse can be used to draw zoom rectangle.

Cursor Control Ctrl+K Mouse can move main and delta cursors.

Pan Ctrl+H Mouse can move (“pan”) plot area inside graph.

Set Extents Dialog

The Set Extents… menu command on the Pan/Zoom menu displays the Set Graph Extents dialog. Double clicking inside any graph will also access the dialog.

Figure 9 Set Graph Extents Dialog

The Set Graph Extents dialog allows the user to enter the minimum and maximum scale values for the visible graphs. This is useful when creating reports of different tests, so that each corresponding graph is scaled alike.


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Cursor Menu

Figure 10 - Cursor Menu

The Cursor menu contains options for moving the cursors, or panning the graphs to the cursors. After panning or lasso zooming the cursors may not be in the visible graph area. To move the main cursor to the center of the current active graph, choose Move Main Cursor to Center. If the delta cursor is not in the current active graph, the Move Delta Cursor to Center option is available, and it will perform a similar function. To pan the graph so the cursor is visible without changing its location, choose Jump to Main Cursor. The Jump to Delta Cursor option will perform the same function if the delta cursor is turned on in the current active graph.

Table 4 - Cursor Menu Functions

Function Toolbar

Icon Action

Move Main Cursor to Center Moves the main cursor to the center of the current active graph.

Move Delta Cursor to Center

Moves the delta cursor to the center of the current active graph. Function available only if delta cursor is turned on in active graph.

Jump to Main Cursor Pans all graphs to display the main cursor.

Jump to Delta Cursor

Pans all graphs to display the delta cursor. Function available only if delta cursor is turned on in active graph.


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Analysis Menu & Toolbar

Figure 11 - Analysis Menu

Figure 12 Analysis Toolbar

The Analysis menu and toolbar provide the tools for placing the micturition cycle definition marks, and performing the micturition cycle analysis.

Table 5 - Analysis Menu Options


Toolbar Icon Action

Previous Threshold

Ctrl+0 Place Previous Threshold mark at main cursor’s location.

Cycle Start Ctrl+1 Place Cycle Start mark at main cursor’s location.

Pressure Threshold

Ctrl+2 Place Pressure Threshold mark at main cursor’s location.

Cycle End Ctrl+3 Place Cycle End mark at main cursor’s location.

Inputs… Ctrl+I N/A Displays the Analysis Algorithm Inputs dialog. See following section for details.

Analyze Ctrl+A Performs analysis on micturition cycle defined by the four marks.


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Analysis Algorithm Inputs Dialog

Figure 13 - Analysis Algorithm Inputs Dialog

Bladder Pressure Peak Detection Parameters

The analysis algorithm searches between the Micturition Cycle Start and Threshold Pressure marks for non-void contractions. A non-void contraction that fulfills the Bladder Pressure Peak Detection Parameters will be stored in the database and displayed in the Non-Void Contraction Analysis Results table. To be considered a “valid” non-void contraction, it must satisfy the Bladder Pressure Peak Detection constraints. These constraints define the minimum peak height, the minimum peak width, and maximum peak width. Using the parameters shown in the dialog above, a peak that rises at least 3.5 mm Hg, in a time period between 0.75 and 5.0 seconds will be recorded as a non-void contraction.

NOTE: The units of bladder pressure may be millimeters of mercury (mmHg) or centimeters of water (cmH2O) depending on the test imported. The units are displayed on the third line in the MED CMG exported txt file.


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Scale Peak Detection Parameters

Starting at the Micturition Cycle Start mark, the analysis algorithm searches forward in time looking for the first urine drop hitting the scale. This is indicated by a rise in the scale trace that surpasses the Scale Threshold Delta within the Scale Trigger Window time period. The time when the Scale Threshold Delta is exceeded is stored to calculate the Void Rate Index. The scale reading immediately prior to the drop detection is stored as the scale baseline for determining Scale Max.

Scale Max Smoothing Parameters

The analysis algorithm searches forward in time from the Scale Peak Detection to find the maximum scale value. The maximum scale value may be an outlier, which means that it may be an artificial peak caused by hardware or acquisition processing anomaly. After the algorithm finds the maximum scale value, it computes the average of the following points. The size of the comparison set is the Scale Max Window. If the maximum scale value exceeds the Scale Max Window average by more than the Scale Max Variability Percentage, it will be rejected. The Scale Max Window will slide forward in time until the maximum value of the window does not exceed the variability percentage of the average.

Defaults

To use a standard set of algorithm parameters, press the “Defaults” button to load them into the dialog. The default values that ship with the software are a good starting point for rat Cystometry analysis. Mouse analysis may require lower peak bladder values for finding non-void contractions, and perhaps lower Scale Threshold Delta. Once the algorithm parameters have finalized, save the values as defaults for future use.

Save These Values as Defaults

Fill this checkbox if you would like the values shown on the dialog stored as the new default values when the OK button is pressed.

Disable Scale Checking

If the Disable Scale Checking option is selected, the scale trace will not be used during analysis. Any void contractions found with the Scale Checking disabled will have a Scale Max and Void Rat Index values of 0. The time of the contraction will be the point of peak bladder pressure between the Threshold Pressure (2) mark and Micturition End (3) mark.


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Analysis Results Tables

Figure 14 - Analysis Results

At the bottom of the application window are two data tables that display the results of each micturition cycle’s analysis. A successful analysis process will yield the detection of a single micturition contraction event, and zero or more non-void contractions. The results will be sorted by time in the tables.

The column widths may be adjusted by positioning the mouse cursor on the right edge of the column to be resized. The cursor icon will change to double headed horizontal arrow. Click the left mouse button and drag left or right to resize.

A row in a results table may be deleted. When deleting a Void Contraction, associated Non-Void Contractions will also be deleted. The reciprocal is not true, that is, when deleting non-void contractions, the associated void contraction will not be deleted. To perform a row delete, select the row with the mouse by clicking in the left-most row, then right click to show the context menu, and select “Delete event at time x”.

Import

The Cystometry Analysis software analyzes data acquired by the MED CMG software. Data acquired using MED CMG are saved as txt files. A Cystometry Analysis database may contain the data from one exported txt file. To import a txt file, go to the File menu, and choose Import.

Export

Cystometry Analysis provides tools for exporting graph data or the results data to text file. The data in the exported text files is in tab-delimited format, to facilitate import into other programs for further analysis. Open the File menu, and the Export pop-up menu to reveal the Graph Data and Results Data options.

Export Graph Data

Users may wish to export the data used to create the display graphs. This can be useful for doing specialized analyses using other programs, or, more commonly, for creating graphs used to generate publication-quality figures of representative experimental recordings. To export graph data, choose Graph Data from the File menu’s Export sub-menu. After you specify the text file name and location, the data currently displayed in the visible graphs will be saved in a tab delimited text file. To export the entire data signal from the visible graphs, be sure to zoom out full before performing the export. The format of the exported graph data is described in Table 6.


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Table 6 – Exported Graph Data Format

Line # Information

1 Test title

2 Original data file path

3 Infusion rate (IR), syringe diameter (SD), pressure transducer calibration coefficient (MP), pressure units

4 Exported graph data column headers

5 Test date and time

6+ Time, Graph 1 data, Graph 2 data, Graph 3 data, Graph 4 data, Codes

Export Results Data

The Void Contraction and Non-Void Contraction analysis results may also be exported to a tab-delimited text file. Open the File menu, then the Export sub-menu, and choose Results Data. After you specify the text file name and location, the data displayed in the results tables at the bottom of the analysis screen will be exported. The format of the exported results data is described in Table 7.

Table 7 - Exported Results Data Format

Line # Information

1 Test title

2 Test date

3 “Void Contractions” over time column, “Non-Void Contractions” over time column

4 Void Contraction data column names, and Non-Void Contraction data column names

5+ Void Contraction results data, and Non-Void Contraction results data

It is important to note that Non-Void Contractions will be grouped alongside their corresponding Void Contraction. Remember that a micturition cycle featuring a Void Contraction may or may not have associated Non-Void Contractions. As an example, refer to Figure 15, which shows an exported result text file opened with Microsoft Excel. In the example, there are four Void Contractions, at times 1439.5, 1705.25, 1864.75 and 2302.0 seconds. The first Void Contraction (time 1439.5) has 16 associated Non-Void Contractions. The second Void Contraction (time 1705.25) has no Non-Void Contractions. The third Void Contraction (time 1864.75) has three Non-Void Contractions. The fourth Void Contraction (time 2302.0) has no Non-Void Contractions.


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Figure 15 - Excel Spreadsheet Displaying Exported Results Data

Print

To print a report, choose Print… from the File menu. The report will contain:

• Test Information: title, date, original source file.

• Visible graphs: value at main cursor, value at delta cursor, difference

• Analysis results tables: void and non-void contraction results


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Figure 16 – Sample Cystometry Analysis Report


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CHAPTER 3 Using Cystometry Analysis

To use Cystometry Analysis, perform the following steps:

• Import data (.txt) file created by MED-CMG acquisition software. • Load Bladder Pressure, Infused Total, and Scale traces. • Zoom into micturition cycle to be analyzed. • Set the four Analysis marks. • Set analysis Algorithm parameters. • Run the Analysis.

Import Data

CMG Analysis databases can contain the data from a single test. When the CMG Analysis application starts, a blank database is created and opened, putting the software in the proper configuration to perform an import. If a saved database has been opened, or a test has already been imported into the currently opened database, go to the File menu, and choose New File.

Once the CMG Analysis software has an empty database file open, go to the File menu, and choose Import. Use the Windows standard File Open dialog to select the desired .txt file created by MED-CMG Acquisition. The import process will populate the database with the test information (such as title and original source file name), and the scaled test data.

Load Bladder Pressure, Infused Total, and Scale traces

Go to the Graph menu, and load the Bladder Pressure trace into graph 1, the Infused Total trace into graph 2, and the Scale trace into graph 3.

Zoom into Micturition Cycle

Select the cursor control tool from the Pan/Zoom menu or Analysis tool bar. Move the cursor to the desired micturition cycle. Zoom into the event using the lasso zoom tool, the Zoom In X button on the Analysis tool bar, or the hot key combination Ctrl + +.

Set Analysis Marks

The analysis algorithm requires four marks to be placed on the micturition cycle:

• 0 - Previous Cycle Threshold Pressure • 1 – Micturition Cycle Start • 2 – Threshold Pressure • 3 – Micturition Cycle End


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Figure 17 - Test with Analysis Marks Positioned

Move the main cursor to the Threshold Pressure of the previous micturition cycle and place the Previous Threshold mark (Ctrl+0). Previous Cycle Threshold Pressure is the point in the micturition cycle immediately preceding the focus cycle where the Bladder Pressure trace rise changes from gradual to steep.

Move the main cursor to the beginning of the micturition cycle and place the Micturition Cycle Start mark (Ctrl+1). Place this mark after the peak of the previous micturition cycle, and before any non-void contractions in the focus cycle.

Now place the main cursor at the focus cycle’s Threshold Pressure (Ctrl+2). Again, the threshold pressure is where the rise in bladder pressure changes from gradual to steep.

Move the main cursor to a point past the bladder pressure peak, and past the scale peak, but before any non-void contractions of the following micturition cycle. Place the Micturition Cycle End mark by pressing Ctrl+3.


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Set Analysis Parameters

Figure 18 - Analysis Algorithm Inputs

To find the non-void contractions, and the maximum scale value, the analysis algorithm requires several user-defined values. Go to the Analysis menu, and select Inputs to access the dialog seen above. The dialog defines three sets of input parameters. The first section defines the Non-Void Contraction recognition parameters. The second section defines the parameters used to find the start of the scale rise during the micturition cycle. The third section is used to specify the values used during the scale max peak detection. The default values loaded by the Defaults button are a good starting point for analyzing rat Cystometry analysis. See the Interface Reference section for a more detailed discussion of the Analysis Algorithm Inputs dialog.


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Perform Analysis

Now the micturition cycle analysis may be performed. Go to the Analysis menu, and choose Analyze to run the analysis on the marked cycle. The results will be placed in the tables at the bottom of the window.

Figure 19 - Analysis Results

If there is no motion on the scale which exceeds the algorithm inputs Scale Threshold Delta within a time period equal to the Scale Trigger Window, a dialog like that shown in Figure 20 will appear. Note that in the example shown, the maximum scale change in the Scale Trigger Window was only 0.004 grams, and the Scale Threshold Delta was 0.05 grams. Since no valid void contraction was detected, no rows will be added to the Void Contraction or Non-Void Contraction results tables.

Figure 20 - No Motion Detected


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Manage Analysis Results

Delete Results

During data analysis, especially early in the process, the Analysis Algorithm Inputs will be fine-tuned to detect the non-void contractions, scale starts, and scale peaks. This will result in the same micturition cycle being analyzed several times. To remove unwanted rows from the Analysis Results tables, position the mouse in the left-most table column (left of “Time”) so the cursor changes to a horizontal arrow pointing right. Click the left mouse button to select the row, then the right mouse button to show a context menu as seen in the following figure.

Figure 21 - Analysis Results Table Context Menu

Select “Go to time …” to move the main cursor to the indicated time. Select the “Delete event at time …” option to delete the results row from the table.

NOTE: When deleting Void Contractions, any “associated” Non-Void contractions will also be deleted. That is, the Non-Void Contractions from the deleted Void Contraction micturition cycle will be removed from the Non-Void Contractions analysis results table. This is NOT reciprocal while deleting Non-Void Contractions. In other words, if a Non-Void Contraction is deleted, the associated Void Contraction finding is unaffected.

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SOF-552 CYSTOMETRY DATA ANALYSIS - Med Associates...Required Data Signal Traces The analysis algorithm uses the Bladder Pressure, Infused Volume and Scale traces. These signals, among