Sirius™ version 6.0

• Sirius™ is a software package for multivariate data analysis and experimental design.

• Application areas:– Spectral analysis and calibration– Industrial process analysis and optimisation– Quality control– Marketing and insurance analysis

Sirius™ 6.0 - New Features

• Full 32 bit application , Windows95 and NT 3.51 or higher

• Larger matrices, improved speed• New interface, featuring Windows95 like interface• New import features, Excel,Lotus. Matlab, JCAMP,

EChip• Improved spreadsheet editor for raw data,

transformed data and subset editing• New graphics including 3D• New graphical editor• New Experimental Design package• continuing….

Sirius™ 6.0 - New Features

• New reporting facility including easy transfer to other Windows application

• Improved pre-processing facilities• New documentation:

– Sirius User Guide– Sirius Reference Guide– Sirius Tutorials, 8 tutorials covering the major areas

of MDA• New Help system

Sirius™ 6.0 - Methods

PCA - Principal Component Analysis PLS - Partial Least Squares Regression PCR - Principal Component Regression SIMCA - Soft Independent Modeling of Class Analogies MOP - Marker Object Projections MVP - Marker Variable Projections

Sirius™ 6.0 - Experimental Design

• Fully integrated with all other parts of the application

• Methods– Fractional, – Full factorial– Central Composite Designs.

Sirius™ 6.0 - Pre-Processing

Roots Logarithmic Block normalising Normalising using internal

standard Log-centering

Standard Transform Options Special Transform Options

Absorbance to reflectance Absorbance to Kubelka-

Munck Reflectance to

Absorbance Multiplicative Scatter

Correction Savitzky-Golay

differentiation

Sirius™ 6.0 - Scaling

Standardising Division by standard deviation + constant Block standardising Division by mean Division by mean + constant Set your own weights

Sirius™ 6.0 - Editor

• Spreadsheet editor for manipulation of data– Raw data– Transformed data– Subset

• Pre-inspection of dataset/subset– Univariate statistics– PCA

• Correlation analysis• Graphics, bivariate, normal plot, histogram,

dendrogram and more

Sirius™ 6.0 - Graphics

2D raw/transformed data 3D raw(transformed data Score and loading plot Biplot Score dendrogram Score vs. objects Loadings vs. variables Residuals Covariance graphs Predicted vs. measured

response Regression coefficients Target coefficients Contour plot

Sirius™ 6.0 - Software Interaction

• ASCII • Excel (XLS)• Lotus 1-2-3 (WKx)• JCAMP• MATLAB• LTIndusties

• Echip

Sirius™ 6.0 - Requirements

Personal computer(486SX or higher) running

Windows 95 or Windows NT or higher

16 MB of memory (32MB recommended)

8 MB of free hard-disk space required

SirEnviron™ version 1.0

An integrated Application for

Environmental Studies.

Focus:

• Monitoring

• Survey design

• Connecting Biology and Chemistry

SirEnviron™ version 1.0

• Visual interface– User-friendly, using

nomenclature like stations, species ..

– Inspecting dataset– Defining reference

set– Graphics, including

contour plot

• Windows95 and NT

CDI index

SirEnviron™ version 1.0

• Includes existing methods like:

– Various univariate indexes• Shannon-Wiener - Diversity• Peilous’s J - Evenness• Hurlbert ES(100) - Deversity

– Dendrogram• Bray-Curtis and Eucledan

– Non-metric Multi-Dimensional Scaling (MDS)

SirEnviron™ version 1.0

• Includes new methods like:

– PCA, CA, SIMCA, Score Dendrogram.– SIMCA - Community Disturbance Index

(CDI)– Connecting Biology and Chemistry using

PLS