Bpupdepartments.ac.in/syllabi/Academic Session 2016-17... · Web viewBiofertilizers: General account of microbes used as Biofertilizers, industrial production of Rhizobium inoculants,

B.Sc.(Industrial Microbiology) Part-III (Semester V & VI) (Sessions 2016-17, 2017-18 and 2018-19)

External Marks

Sessional work (Internal Assessment)

Semester V: Theory Paper-I: Chemistry I (Inorganic Chemistry)

30 8 (Attendance: 2+ Assignment:3 + House Test: 3

Theory Paper - II: Chemistry II (Organic Chemistry) 30 8 (Attendance: 2+ Assignment:3 +

House Test: 3

Theory Paper -III: Chemistry III (Physical Chemistry) 30 8 (Attendance: 2+ Assignment:3 +

House Test: 3

Theory Paper - IV: Food & Industrial Microbiology A 60 15 (Attendance: 3+ Assignment:6 +

House Test: 6

Theory Paper - V: Food & Industrial Microbiology B 60 15 (Attendance:3+ Assignment:6 +

House Test: 6

Theory Paper - VI: Biostatistics, Computer Applications and Bio-informatics

40 10 (Attendance: 3+ Assignment:3 + House Test: 4

Practical Paper-IPertaining to theory paper I, II and III

36 Nil

Practical Paper-IIPertaining to theory paper IV & V

50 Nil

Total 336 64

Grand Total 400

Semester VI: Theory Paper-I : Chemistry I (Inorganic Chemistry)

30 8 (Attendance: 2+ Assignment:3 + House Test: 3)

Theory Paper - II: Chemistry II (Organic Chemistry) 30 8 (Attendance: 2+ Assignment:3 +

House Test: 3)

Theory Paper - III: Chemistry III (Physical Chemistry) 30 8 (Attendance: 2+ Assignment:3 +

House Test: 3

Theory Paper - IV: Environmental

Microbiology & Microbial

Technology A60 15(Attendance: 3+ Assignment:6 +

House Test: 6

Theory Paper - V: Environmental

Microbiology & Microbial

Technology B60 15(Attendance: 3+ Assignment:6 +

House Test: 6

Theory Paper -VI: Entrepreneurship

Development 40 10(Attendance: 3+ Assignment:3 + House Test: 4

Practical-IPertaining to theory paper I, II & III

36 Nil

Practical-IIPertaining to theory paper IV & V

50 Nil

Total 336 64

Grand Total 400

B.Sc.-III (Industrial Microbioogy) Syllabus 2016-17, 2017-18 & 2018-19

SEMESTER-V THEORY PAPER I : Chemistry-I (INORGANIC CHEMISTRY)

Max. Marks: 38 Pass Marks: 35%Semester Paper=30 Time: 3 hrsInternal Assessment=8 30 Hrs (2 Hrs/Week)

INSTRUCTIONS FOR THE PAPER SETTER

The question paper will consist of three sections: A, B and C. Sections A and B will have four questions each from the respective section of the syllabus and will carry 5 marks each. Section C will consist of 5 short answer questions that will cover the entire syllabus and will be of 2 marks each. Use of scientific non-programmable calculator is allowed.

INSTRUCTIONS FOR THE CANDIDATESCandidates are required to attempt five questions (Section C all questions being

compulsory) selecting two questions from each of A & B Sections.

SECTION-AI. Metal-ligand Bonding in Transition Metal Complexes.

Limitations of valence bond theory, an elementary idea of crystal- field theory, crystal field splitting in octahedral, tetrahedral and square planar complexes, factors affecting the crystal-field parameters.

II. Thermodynamic and Kinetic Aspects of Metal Complexes A brief outline of thermodynamic stability of metal complexes and factors affecting the stability, substitution reactions of square planar complexes.

SECTION -BIII. Magnetic Properties of Transition Metal Complexes

Types of magnetic behaviour, methods of determining magnetic susceptibility, spin-only formula, L-S coupling, Correlation of µs and µeff values, orbital contribution to magnetic moment, application of magnetic moment data for 3d-metal complexes.

IV. Electronic Spectra of Transition Metal Complexes. Types of electronic transitions, selection rules for d-d transitions, spectroscopic ground states, spectrochemical series. Orgel-energy level diagram for d1 and d9 states, discussion of electronic spectrum of [Ti (H2 O)6]3+ complexion.

THEORY PAPER II: Chemistry-II (ORGANIC CHEMISTRY)






SECTION-AI. Spectroscopy

Nuclear magnetic resonance ( NMR) spectroscopy. Proton magnetic resonance (1H NMR) spectroscopy, nuclear shielding and deshielding, chemical shift and molecular structure, spin-spin splitting and coupling constants, areas of signals interpretation of PMR spectra of simple organic molecules such as ethyl bromide, ethanol, acetaldehyde, 1,1,2 tribromoethane, ethyl acetate, toluene and acetophenone.

II. Electromagnetic spectrum: Absorption Spectra Ultraviolet (UV) absorption spectroscopy-absorption laws (Beer-Lambert's law, Molar absorptivity, presentation and analysis ofUV Spectra, types of electronic transitions, effect of conjugation. Concept of chromophore and auxochrome. Bathochromic, hypsochromic, hyperchromic and hypochromic shifts. UV spectra of conjugated enes and enones.

SECTION - BIII. Infrared (IR)

Infrared (IR) absorption spectroscopy-molecular vibrations, Hooke's law, Selection rules, intensity and position of IR bands, measurement of lR spectrum, fingerprint region, characteristic absorption of various functional groups and Interpretation of IR spectra of simple organic compounds.

Problems pertaining to the structure elucidation of simple organic compounds using UV, IR, and PMR spectroscopic techniques.

IV. Organometallic Compounds Organomagnesium Compounds The Grignard reagents formation, structure and chemical reactions. Organozinc compounds: formation and chemical reactions. Organolithium compounds: formation and chemical reactions.

V. Organosulphur Compounds Nomenclature, structural features, methods of formation and chemical reactions of thiols, thioethers, sulphonic acids, and sulphonamides.

THEORY PAPER III: Chemistry-III: (PHYSICAL CHEMISTRY)Max. Marks: 38 Pass Marks: 35%Semester Paper=30 Time: 3 hrsInternal Assessment=8 30 Hrs (2 Hrs/Week)





SECTION-AI. Elementary Quantum Mechanics

Black-body radiations, Planck's radiation law, photoelectric effect, heat capacity of solids. Sinusoidal wave equation Hamiltonian operator, Schrodingcr wave equation and its importance, physical interpretation of the wave function, postulates of quantum mechanics, particle in a one dimensional box.Sehrodinger wave equation for H-atom, separation into three equations (without derivation), quantum numbers and their importance, hydrogen like wave functions, radial wave functions, angular wave functions.

SECTION-BII. Spectroscopy

Introduction : Electromagnetic radiation, regions of spectrum, basic features of different spectrometers, statement of Born-Oppenheimer approximation, degrees of freedom.

Rotational Spectrum : Diatomic molecules. Energy levels of a rigid rotor (semi-classical principles), selection rules, spectral intensity, determination of bond length, qualitative description of non-rigid rotor, isotope effect.

Vibrational Spectrum: Infrared spectrum: Energy levels of simple harmonic oscillator, selection rules, pure vibrational spectrum, intensity, determination of force constant and qualitative relation of force constant and bond energies, effect of anharmonic motion and isotope on the spectrum, idea of vibrational frequencies of different functional groups.

B.Sc. INDUSTRIAL MICROBIOLOGY: PART–III (SEMESTER-V)

THEORY PAPER IV: FOOD AND INDUSTRIAL MICROBIOLOGY-AMax. Marks: 75 Pass Marks: 35%Semester Paper=60 Time: 3 hrsInternal Assessment=15 45 Hrs (3 Hrs/Week)

INSTRUCTIONS FOR PAPER SETTER

The question paper will consist of three sections A, B and C. Section A and B will have four questions each from the respective sections of the syllabus and will carry 12 marks each. Section C will consist of 12 short-answer type questions which will cover the entire syllabus uniformly and will carry 12 marks in all.

INSTRUCTIONS FOR CANDIDATESCandidates are required to attempt four questions selecting two questions from

each section A and B. The entire section C is compulsory.

Section A1. Food as substrate for microorganisms: pH, moisture, water activity, oxidation –

reduction potential, nutrient content, inhibitory substances and biological structure, various factors affecting growth and activity of microorganisms in foods.

2. Different type of microorganisms associated with foods, general characteristics, classification, identification and importance, contamination sources, role in food spoilage and desirable fermentations.

3. Principals of food preservation: asepsis, removal of water, anaerobic conditions, preservation using high temperature, drying, low temperature, food additives, radiations, alternate methods for food preservation.

Section B4. Microbiology of cereals and cereal based products, food and vegetables meat and

meat products, milk and milk products: sources of contamination, spoilages and preservations.

5. Food borne diseases: Bacteria, mold, viruses- action, transmission, prevention and control.

6. Cleaning and sanitation of food processing plants, Microbiological quality of foods, microbiological criteria and standards of foods, good manufacturing practice, HACCP, ISO.

RECOMMENDED BOOKS:1. Frazier. WC and Westerner DC 1998. Food Microbiology, Mc Graw Hill Inc. New

York.2. Jay, JM. 1986. Modern Food Microbiology, Nostrand Rein hold.3. Casida LE . Industrial Microbiology, New Age International Publishers.4. Prescott and Dunn.Industrial Microbiology, 5. Peppler and Perlman. Microbial Technology Vol.-I and Vol.-II, by, Academic press.6. Stanbury and Whitaker Principals of fermentation Technology


THEORY PAPER V: FOOD AND INDUSTRIAL MICROBIOLOGY-B






Section A

1. Historical development in industrial microbiology. 2. Isolation and screening of industrially important microorganisms, genetic

modification and preservation of industrial microorganisms.3. Definition of fermentation, fermentor or Bioreactor; brief description and design

of fermentor (batch, fed batch and continuous fermentors), fermentation media and its optimization, Large-scale inoculum preparation.

Section B

4. Primary products of microbial metabolism: industrial alcohol, beer, whisky, wine vinegar, single cell protein, amino acids (insane and glutamic acid), production of citric acid, lactic acid, acetic acid.

5. Secondary products of microbial metabolism: Antibiotics (penicillin, tetracycline), microbial transformation of steroids and sterols.

6. Fermentation economics, product recovery (Down streaming) of alcohols, citric acid lactic acid, enzymes, antibiotics, alkaloids and steroids.

RECOMMENDED BOOKS:

1. Frazier. WC and Westerner DC 1998. Food Microbiology, Mc Graw Hill Inc. New York.

2. Jay, JM. 1986. Modern Food Microbiology, Nostrand Rein hold.3. Casida LE . Industrial Microbiology, New Age International Publishers.4. Prescott and Dunn.Industrial Microbiology, 5. Peppler and Perlman. Microbial Technology Vol.-I and Vol.-II, by, Academic

press.6. Stanbury and Whitaker Principals of fermentation Technology


THEORY PAPER VI: BIOSTATISTICS, COMPUTER APPLICATIONS AND BIO-INFORMATICS

M. Marks: 50 45 Hrs (3 Hrs/Week)Semester Paper: 40 Pass Marks: 35% Internal Assessment: 10 Time allowed: 3 hours





Section-A1. Statistical Methods: Collection of Data, distribution and graphical representation,

measurement of central tendency 2. Distribution of data and their fittings- binomials, poison and normal distribution,

correlation and regression3. Sample statistic and parameters, population and null hypothesis, level of

significance, X2- test, t-test 4. Analysis of variance- ANOVA with linear models, one-way, II way ANOVA

Section-B5. Computer Basic: Introduction, Input and output devices, computer memory,

hardware and software of computer, MS- window, 6. Internet and web browser, mail, and file transfer, office2000/XP including

MS word, Excel, Power Point.7. Bio- Informatics: virtual Library: searching MEDLINE on the pub med system

from the National Center for Biotechnology and Information, Assessing full text journals on the internet and printing antics

RECOMMENDED BOOKS

Statistical Method by SP Gupta, Sultan Chand and Sons.Basic statistic by Snedekor and Kochran Biostatistics by Arora PN and Malhan PK Himalaya Pub. House, Mumbai

Useful sites on the internet Database (gene bank) and search tools and software at: http://www.ncbi.nih.gov Restriction Enzyme site digestion webcutter 2.0 at

http://www.firstmarket.com/cutter/cut2.html.

B.Sc. III, (Industrial Microbioogy) Semester VPRACTICALS

Practical-I (Pertaining to theory paper -I, II and II)

Max. Marks : 36 Time: 4 Hrs.

6 Periods/Week

INSTRUCTIONS FOR EXAMINERS AND CANDIDATESCandidate are required to prepare inorganic complex and synthesise organic

compound. The candidate will perform experiments. Distribution of marks will be as under:

1. Inorganic Complex = 12 (5 for initial write up) 2. Organic Synthesis = 12 (5 for initial write up)3. Viva-Voce = 8 4. Note Books = 4

Total =36 Synthesis and Analysis (a) Preparation of sodium trioxalatoferrate(III), Na3 [Fe(C2O4)3] and determination of its composition by permagnometry. (b) Preparation of Ni-DMG complex, [Ni (DMG)2]2+ (c) Preparation of copper tetra-ammine complex. [Cu (NH3) 4] S04. (d) Preparation of cis-and trans-bis(oxalato)diaquachromate(III) ion.

Synthesis of Organic Compounds (a) Iodoform from ethanol and acetone

(b) Aromatic electrophlic substitution of benzene1. p-nitroacetanilide 2. 2,4,6-tribromophenol

Diazotization/Coupling 3. Preparation of methyl orange and methyl red 4. Preparation of benzoic acid from toluene 5. Reduction 6. Preparation of m-nitroaniline from m-dinitrobenzene

Practical Paper-II (Pertaining to theory papers IV & V)


6 Periods/Week

1. Enumeration of microorganisms present in spoiled bread dried milk, condensed Milk, jam, butter, cheese etc

2. Identification of micro flora involved in spoilage though microscopic method3 Study the microbiological quality of raw and pasteurized milk4 Examination of microbiological quality of fruits and vegetables, meat and meat

products, egg, poultry, fish products by SPC, specific micro-organism counts5 Screening of industrially important microorganisms from soil and fruits and

fermented milks.6 Study and sterilization of lab fermentor7 Production of alcohol from molasses.8 Production of lactic acid from cheese whey.9 Product of extra-cellular microbial strains.10 Production of SCP from yeast fungi and bacteria.

NOTE:Candidates will be required to submit their original notebook of lab work initiated

with date by their teacher at the time of practical examination. Students must be taken for excursion to industries, laboratories to have an exposure to daily life use of the subject. Practical examination shall be held in a single morning session from 9:00 a.m. to 1:00 p.m. Practical examination is to be conducted by the two external examiners.

Guidelines pattern for the conduct of practical examination

Max. Marks: 50Practical exercises: 30 marks Internal Assessment: including visit to Industry 10 marksPractical Records: 5 marksViva Voice: 5 marks

B.Sc.-III (Industrial Microbioogy) Syllabus 2016-17, 2017-18 & 2018-19

SEMESTER -VITheory Paper- I : Chemistry-I (INORGANIC CHEMISTRY)





compulsory) selecting two questions from each of A & B Sections.SECTION-A

I. Hard and Soft acids and Bases (HSAB) Classification of acids and bases as a hard and soft, Pearson's HSAB concept,

acid-base strength and hardness and softness. Symbiosis, theoretical basis of hardness and softness, electronegativity and hardness and softness.

II. Bioinorganic Chemistry Essential and trace elements in biological processes, metalloporphyrins with

special reference to haemoglobin and myoglobin. Biological role of alkali and alkaline earth metal ions with special reference to Ca+2, Nitrogen fixation.

SECTION-BIII. Silicones and Phosphazenes

Silicones and Phosphazenes as examples of inorganic polymers,nature of bonding in triphosphazenes.

IV. Organometallic Chemistry Definition, Nomenclature and classification of organometallic compounds.

Preparation, properties, bonding and applications of alkyls,of' Li, Al, Hg, Sn and Ti, a brief account of metal-ethylene complexes and homogeneous hydrogenation, mononuclear carbonyls and the nature of bonding in metal carbonyls.

Theory Paper- II: Chemistry-II (ORGANIC CHEMISTRY)


3 Periods/Week

INSTRUCTIONS FOR THE PAPER SETTERThe question paper will consist of three sections: A, B and C. Sections A and B

will have four questions each from the respective section of the syllabus and will carry 5 marks each. Section C will consist of 5 short answer questions that will cover the entire syllabus and will be of 2 marks each. Use of scientific non-programmable calculator is allowed.


compulsory) selecting two questions from each of A & B Sections.SECTION-A

I. Heterocyclic Compounds Introduction: Molecular orbital picture and aromatic characteristics of pyrrole,

furan, thiophene and pyridine. Methods of synthesis and chemical reactions with particular emphasis on mechanism of electrophlic substitution. Mechanism of nucleophlic substitution reaction in pyridine derivatives. Comparison of basicity of pyridine, piperidine and pyrrole.

Introduction to condensed five and six membered heterocycles.Preparation and reactions of indole, quinoline and isoquinoline wi th special reference to Fischer indole synthesis, Skraup synthesis and Bischler- Napieralski synthesis. Mechanism of electrophlic substitution reactions of indole, quinoline and isoquinoline.

II. Synthesis of Polymers

Ziegler-Natta polymerziation and vinyl polymers. Condensation or step growth polymerziation. Urea formaldhehyde resins, epoxy resins and polyurethanes. Natural and synthetic rubbers.

III. Organic Synthesis Via EnolatcsAcidity of α-hydrogens, alkylation of diethyl malonate and ethyl acetoacetate.

Synthesis of ethyl acetoacetate: the Claisen condensation. Keto-enol tautomerism of ethyl acetoacetate. Alkylation and acylation of enamines.

SECTION - BIV. Carbohydrates

Classification and nomenclature, Monosaccharides, mechanism of osazone formation, interconversion of glucose and fructose, chain lengthening and chain shortening of aldoses.

Configuration of monosaccharides. Erythro and threodiastereomers. Conversion of glucose into mannose. Formation of glycosides, ethers, and esters. Determination of

ring size of monosaccharides. Cyclic structure of D ( + )-glucose. Mechanism of mutarotation.

Structures of ribose and deoxyribose. An introduction to disaccharides ( maltose, sucrose and lactose) and

polysaccharide starch and cellulose without involving structure determination.

V. Amino Acids, Pcptidcs, Proteins.Classification, structure and stereochemistry of amino acids. Acid base behaviour,

isoelectric point and electrophoresis. Preparation and reactions of α-amino acids. Structure and nomenclature of peptides and proteins. Classification of proteins.

Peptide structure determination, end group analysis, selective hydrolysis of peptides. Classical levels of protein structure. Protein denaturation/renaturation.VI. Nucleic Acids.

Nucleic acids: Introduction, Constituents of nucleic acids Ribonucleosides and ribonucleotides. The double helical structure of DNA.

Theory Paper- III: Chemistry-III (PHYSICAL CHEMISTRY)


3 Periods/Week

INSTRUCTIONS FOR THE PAPER SETTERThe question paper will consist of three sections: A, B and C. Sections A and B

will have four questions each from the respective section of the syllabus and will carry 5 marks each. Section C will consist of 5 short answer questions that will cover the entire syllabus and will be of 2 marks each. Use of scientific non-programmable calculator is allowed.



SECTION-AI. Raman Spectrum :

Concept of polarizability, pure rotational and pure vibrational Raman spectra of diatomic molecules, selection rules.

II. Electronic Spectrum : Concept of potential energy curves for bonding and antibondingmolecular

orbitals, qualitative description of selection rules and Franck-Condon principle. Qualitative description of σ, π and n M.O. their energy levels and their respective transitions.

III. Solid State Definition of space lattice and unit cell. Laws of crystallography-(i) Law of constancy of interfacial angles. (ii) Law of

rationality of indices (iii) Law of symmetry elements in crystals. X-ray diffraction by crystals. Derivation of Bragg's equation. Determination of crystal structure ofNaCI, KCI and CsCI (Laue's method and powder method).

SECTION-BIV. Photochemistry

Interaction of radiation with matter, difference between thermal and photochemical process. Laws of photochemistry: Grothus-Drapperlaw, Stark-Einstein law, Jablonski diagram depiciting various processes occurring in the excited state, qualitative description of fluorescence, non- radiative processes (internal conversion, intersystem crossing), quantumyield, photosensitized reactions- energy transfer processes (simple examples). Basic concepts of Laser and Maser. Photochemistry of vision and colour.

BOOKS SUGGESTED (THEORY COURSES) 1. Basic Inorganic Chemistry, F.A. Cotton, G Willdson and P.L. Gaus, Wiley. 2. Concise Inorganic Chemistry, J.D. Leee, ELBS. 3. Concept of models of Inorganic Chemistry, B. Douglas, D. McDaniel, and J.

Alexander, Jolin Wiley. 4. Inorganic Chemistry, D. E. Shriver, P. W. Atkins and C.H. Langford, Oxford. 5. Inorganic Chemistry, W. W. Porterfield Addison-Welsey. 6. Inorganic Chemistry, A. G Sharpe, ELBS 7. Inorganic Chemistry, G. L. Miessler and D. A. Tarr, Prentice Hall. 8. Inorganic Chemistry, Morrison and Boyd, Prentice-Hall. 9. Inorganic Chemistry, L.G Wade Jr. Prentice-Hall.10. Fundamentals of Organic Chemistry, Solomons, John Wiley. 11. Organic Chemistry, Vol. I, II & III, S.M. Mukherji, S.P. Singh and R.P. Kapoor,

Wiley Eastern Ltd. (New Age International). 12. Organic Chemistry, F.A Carey, McGraw-Hill, Inc. 13. Introduction to Organic Chemistry, Streitwieser, Healthcock and Kosover and

Kosover, Macmillan. 14. Physical Chemistry, G.M. Barrow, International Student edition, McGraw Hill. 15. University General Chemistry, C.N.R. Rao. Macmillan. 16. Physical Chemistry, R.A Alberty, Wiley Eastern Ltd. 17. The Elements of Physical Chemistry, P. W. Atkins, Oxford. 18. Physical Chemistry Through Problems, S.K. Dogra and S. Dogra, Willey Eastern Ltd.19. Fundamentals of Photochemistry, Rohtga and Mukherji.

BOOKS SUGGESTED (LABORATORY COURSES) 1. Vogel's Qualitative Inorganic Analysis, revised, Svehla, Orienl P Longman. 2. Vogel's Text book of Quantitative Inorganic Analysis (revised), J.Bassett, R. C.

Denney, G.H. Jeffery and J. Mendham, ELBS. 3. Standard Methods of Chemical Analysis, W. W. Scott, The Technical Press. 4. Experimental Inorganic Chemistry, W. G. Palmer, Cambridge. 5. Handbook of Preparative Inorganic Chemistry, Vol. I & II, Brauer, Academic

Press. 6. Inorganic Synthesis, Mc-Graw Hill. 7. Experimental Organic Chemistry, Vol. I & II, P. R. Singh, D.S. Gupta, and Bajpai,

Tata Mc-Graw Hill. 8. Laboratory Manual In Organic Chemistry, R. K. Bansal, Wiley Eastern. 9. Vogel's Textbook of Practical Organic Chemistry, B. S. Furniss, Al Hannaford, V.

frogers, P.W.G. Smith and AR. Tatchell, ELBS 10. Experiments in General Chemistry, C.N.R. Rao and U.C. Agarwal, East-West

Press. 11. Experiments in Physical Chemistry, R.C. Das, and B. Behra, Tata Mc-graw Hill. 12. Advanced Practical Physical Chemistry, J.B. Yadav, Goel Publishing House.13. Advanced Exp. Chemistry, Vol. I-Physical, J.N. Gurutu and R. Kapoor, S. Chand &

Co.14. Selected Exp. in Physical Chemistry, N.G. Mukherjee, J.N. Ghose & Sons.15. Exp. in Physical Chemistry, J.C. Ghosh, Bharti Bhavan.

B.Sc. INDUSTRIAL MICROBIOLOGY: PART–III (SEMESTER-VI)

THEORY PAPER - IV: ENVIRONMENTAL MICROBIOLOGY AND MICROBIAL TECHNOLOGY-A

Max. Marks: 75 Pass Marks: 35%Semester Paper=60 Time: 3 hrsInternal Assessment=15 Lecture to be delivered: 80

3 Periods/Week





Section A

1. Micro environmental and microbial ecology: Type and distribution of microorganisms in ecological systems: air, water and soil, structure of atmosphere and microbial distribution; forms of water, abundance and distribution of microorganism in water bodies.

2. An account of air borne and water borne diseases.3. Microbial ecosystem: biology of ecosystem, succession of the organism in

ecosystems, adaptation in ecosystem, microbes: biomass and activities immurement, direct viable counts, biochemical methods, measurement of microbial biomass.

4. Microbial interactions: concept of positive, negative and neutral interactions, parasitism, predation, neutralism, commensalisms, amensalism, mutualism, synergism and competition.

Section B

5. Biofertilizers: General account of microbes used as Biofertilizers, industrial production of Rhizobium inoculants, cyanobacteria, Azolla and Anabaena.

6. N2 fixation, role of blue green algae in rice cultivation, assessment of N2 –fixing ability of different strains, direct and indirect methods, packing, quality control and storage, method of application.

7. Bioremediations: Nature and effects of pesticides potency, persistence detoxifying metabolism of pesticides, Biomagnifications, microbial leaching, direct and indirect bacterial leaching

8. Waste Water Treatment- types, treatment (physical, chemical and biological) Biological- aerobic, anaerobic (primary, secondary and tertiary), trickling filters, activated sludge, and oxidation ponds, lagoons

RECOMMENDED BOOKS

1. Microbial Ecology- fundamentals and Application by Atlas, RM and Berth, M- Addison Wesley, Phillburry.

2. Principles of Microbial Ecology by Brock, TD Prentice Hall USA.3. Environmental Biotechnology by Grant and Long, PE Blockie Publisher London.4. Bioreactors Biotechnology: A practical approach by Scraggy AH, Ellis Harwood

Ltd.5. Principle of Biotechnology by Alan Wiseman.6. Application of Biotechnology by Robert Qwllette and Paul Cheremisin 7. Molecular Biology and Biotechnology: Smith and Wood, Chapman and Hall Ltd.,

1991.8. Recombinant DNA: A Short Course: J.A. Watson, J. Tooze and D.T. Durtz,

Scientific American Books, 1983.9. Gene Manipulation: Old and Primrose, 2000.


THEORY PAPER -V: ENVIRONMENTAL MICROBIOLOGY AND MICROBIAL TECHNOLOGY-B

Max. Marks: 75 Pass Marks: 35%Semester Paper=60 Time: 3 hrsInternal Assessment=15 Lecture to be delivered: 80

3 Periods/Week




each section A and B. The entire section C is compulsory.SECTION – A

1. Introduction to R-DNA technologyTools of R-DNA technology : DNA cutting tool, restriction enzymes, DNA end modifying tools , 3’ nucleotidyl transferase, 5- nucleotidyl kinase, alkaline phosphatase, T4 DNA ligase, joining DNA fragments.

2. Techniques for Genetic Engineering: Southern Blotting, Northern Blotting, Western Blotting, DNA Sequencing: Maxam- Gilbert technique, sanger’s Dideoxy Chain Termination, Polymerase Chain Reaction, Agarose gel electrophoresis.

3. Introduction to Cloning Genes: Cloning by direct cDNA cloning.Vectors: plasmids, bacteriophages, shuttle vectors, retro viruses, purification of vector DNA

4. Transformation Techniques: Competent E. coli cells, yeast, Spheroplasts, Bacillus protoplast, CaCl2 method, ballistic gun, electroporation, phage transfection

SECTION-B5. Cloning in E. coli and yeast and selection of Transformants in respective

organism.6. Cloning in Plant Cells and selection of Transformants. 7. Application of R-DNA Technology in Agriculture and Industry. 8. Application of R-DNA Technology in Medicine, RFLP and Prenatal diagnosis.

RECOMMENDED BOOKS

1. Microbial Ecology- fundamentals and Application by Atlas, RM and Berth, M- Addison Wesley, Phillburry.

2. Principles of Microbial Ecology by Brock, TD Prentice Hall USA.

3. Environmental Biotechnology by Grant and Long, PE Blockie Publisher London.

4. Bioreactors Biotechnology: A practical approach by Scraggy AH, Ellis Harwood Ltd.

5. Principle of Biotechnology by Alan Wiseman.6. Application of Biotechnology by Robert Qwllette and Paul Cheremisin 7. Molecular Biology and Biotechnology: Smith and Wood, Chapman and Hall

Ltd., 1991.8. Recombinant DNA: A Short Course: J.A. Watson, J. Tooze and D.T. Durtz,

Scientific American Books, 1983.9. Gene Manipulation: Old and Primrose, 2000.


THEORY PAPER VI: ENTREPRENEURSHIP DEVELOPMENT

M. Marks: 50 Lecturer to be delivered: 45Semester Paper: 40 Internal Assessment: 10Pass Marks: 35% Time allowed: 3 hours



INSTRUCTIONS FOR CANDIDATES

Candidates are required to attempt four questions selecting two questions from each section A and B. The entire section C is compulsory.

Section A1. Entrepreneurship: Introduction, characteristics, need and scope, scheme for

technical entrepreneurs, finding opportunities, exposure to different food and Dairy industries, Pharmaceutical Industries and Analysis of their resources, import substitutes and export promotion, various techniques of market survey.

2. Project formulation: Its needs, scope and approaches, product selection criteria, choice of technology, plant and equipments financial procedures and their signification, books of accounts, financial statements and fund flow analysis; energy input; resources (labours, machinery and materials) and their management; project evolution review techniques (PERT) and critical path method (CPM)

Section B3. Process planning and Management: Techno economies feasibility of the project

creativity and innovation; plant payout and process planning for the products; quality assurance and testing of products; elemental knowledge of marketing and sales management, marketing strategies, packaging, advertising, after sale services; costing and product pricing, sickness in small scale services; costing and scale industries and their remedies, stress management and positive reinforcement

4. License registration, insurances coverage, and important provisions of factory act: partnership act, sale tax, and income tax and excise rules; social responsibilities and business ethics.

RECOMMENDED BOOKS1. Entrepreneurship of Small Scale Industries; Concept Growth and Management by

Deshpande, MV Deep and Deep publication New Delhi2. Planning an Industrial Unit by Vyas JN Neelkanth, Ahmedabad3. Developing Enterpreneuship- A hand book by Rao TV and Pareek U, Learning

systems, New Delhi.4. Practice of Entrepreneurship by Meredith GG and Nelson R. ILO Geneva 19825. Personal Efficacy in Developing Entrepreneurship by Pareek U and Rao TV.

B.Sc. III, (Industrial Microbioogy) Semester VIPRACTICALS

Practical-I (Pertaining to theory paper -I, II and II)


6 Periods/Week

INSTRUCTIONS FOR EXAMINERS AND CANDIDATESCandidate are required to prepare perform column Chromatography experiment

and the physical experiments. The candidate will perform experiments from physical, chemistry. Distribution of marks will be as under:

1. Column Chromatography = 052. Models = 053. Physical Experiments = 16* (4 for initial writeup

for both experiments)* (Full credit may be given for error upto 10% and one mark may be deducted for additional 5% error.)

4. Viva-Voce = 06 5. Note Book = 04

Laboratory Techniques 1. Column Chromatography 2. Separation of fluorescein and methylene blue. 3. Separation of leaf pigments from spinach leaves. 4. Physical Experiments

(a) To determine the strength of the given acid conductometrically using standard alkali solution.

(b) To determine the solubility and solubility product of a given sparingly soluble electrolyte conductometrically.

(c) To study the saponification of ethyl acetate conductometrically. (d) To determine the ionisation constant of a weak acid conductometrically. (e) To determine the strength of the given acid solution pH- metrically by using standard

alkali solution. (f) To determine the molar refraction of methanol, ethanol and propanol. (g) To study the distribution of benzoic acid between benzene and water, and ether and water.(h) Knowledge of Stereochemical Study of Organic Compounds.

Rand S configuration of optical isomers. E, Z configuration of geometrical isomers. Conformational analysis of cyclohexanes and substituted cyclohexanes.

Practical Paper -II (Pertaining to Theory Paper IV & V)Max. Marks : 50 Time: 4 Hrs.

6 Periods/Week

1. Enumeration of microbes from different ecosystems.2. Effect of different temperature on yeast growth3. Isolation of N2 fixing, nitrifying bacteria.4. Study the BOD and COD levels of different water system5. Bacteriological analysis of water by presumptive, confirmatory and completed

tests6. Protoplast formation, regeneration and fusion 7. DNA isolation and agarose gel electrophoresis8. Curing of plasmid9. Counting total number of viable bacteria in a bio-fertilizer packet 10. Counting the most probable number (MPN) of Rhizobium 11. Isolation of phosphate solubilizing microorganisms. 12. Mass production and carrier mixing of Rhizobium inoculants

NOTE:

Candidates will be required to submit their original notebook of lab work initiated with date by their teacher at the time of practical examination. Students must be taken for excursion to industries, laboratories to have an exposure to daily life use of the subject. Practical examination shall be held in a single morning session from 9:00 a.m. to 1:00 p.m. Practical examination is to be conducted by the two external examiners.

Guidelines pattern for the conduct of practical examination.

Max. Marks: 50Practical exercises : 25 marks. Internal Assessment: including visit to industry 15 marksPractical Records: 5 marksViva Voice: 5 marks

Documents

Bpupdepartments.ac.in/syllabi/Academic Session 2016-17... · Web viewBiofertilizers: General account of microbes used as Biofertilizers, industrial production of Rhizobium inoculants,