Serology Questions 100 Points With Answers

7/30/2019 Serology Questions 100 Points With Answers

1/4

ANSWER SHEET

1. PRIMARY REACTION is the specific recognition and combination of antigen with antibody2. SECONDARY REACTION is the conformation of amino acid chain resulting from inter chain

hydrogen bonding

3. In dean webb or alpha titration technique, there is an excess of ANTIGEN during the prozonephase

4. It is the pH at which proteins exist in an ampholyte state? ISOELECTRIC POINT5. During agglutination reaction, the involved particulate antigen is called AGGLUTINOGEN6. In radioimmunoassay, the sold crystal gamma counter is used to quantify GAMMA RAY

emissions

7. The common substrate used for the enzyme peroxidise? TETRAMETHYLBENZIDINE ODIANISIDINE

8. What is the enzyme produced from E. coli? B-GALACTOSIDASE9. What is the source of the enzyme alkaline phosphatise? BOVINE INTESTINES10.What is the ANTIBODY class used for the neutralization of endotoxin? IgM11.During agglutination, the initial sensitization phase occurs followed by lattice formation TRUE12.During precipitation sensitization occurs in the area of optimal ratio between antigen and

antibody FALSE, WRONG WORD= SENSITIZATION, RIGHT WORD = LATTICE FORMATION

13. In CFT the antigen control contains the antibody and its end result is lysisFALSE, WRONG WORDS= CONTAINS THE ANTIBODY, RIGHT WORD=HAS NO ANTIBODY

14. In CFT, the haemolytic system control is positive for the antigen and the antibody hence lysis isthe end result FALSE, WRONG WORD=IS POSITIVE, RIGHT WORD=IS NEGATIVE

15.Double diffusion-single dimension gel precipitation test by Oakley and fulthrope determines thenumber of antigen and antibody in the specimen TRUE

16.For serologic application of the principle of precipitation are:a) CRP DETERMINATIONb) ASCOLI RING TESTc) CRPd) TESTING FOR ADULTERATED FOOD

17. In electrophoresis, movement is dependent on:a) THE TAGGING BY NON-SEPARATION OF BOUND FROM FREE FRACTIONb) DEGREE OF IONIZATION OF PROTEIN AT pH OF BUFFER SOLUTIONc) THE USE OF ORGANIC SOLVENTSd) THE USE OF ABSORPTION MEDIA

18. In radial immunodiffusion, consider the following:a) POLYMERIC Ig DIFFUSE SLOWLY AND THUS MAY LEAD TO UNDERESTIMATIONb) HIGH MOL. WEIGHT IMMUNE COMPLEXES RESULT TO FALSELY LOW VALUESc) FALSE HIGH VALUES FOR LOW MOLECULAR WEIGHT Igd) REVERSE PRECIPITATION MAY OCCUR IN CONDITIONS WHERE THE TESTED SERUM

CONTAINS ANTI-Ig


2/4

19.Application of RICE test:a) TREPONEMA PALLIDUM CFb) REITER PROTEIN CFc) WASSERMAN-KOLMER CFd) BLASTOGENESIS CF

20.Application of indirect immunofluorescencea) FADFb) FITCc) DANSYLd) BAIF

NON ARE CORRECT

21.5 points. For heterogenous RIA give 5 methods that may result to the precipitation ofproteins/separation step

Salting out technique/farr technique Use organic solvents Double antibody technique Use absorption media Use of capture/immobilizing media/solid phase

1. Serodiagnostic test for Myco. Pneumonia:a) CFTb) EIAc) PCRd) DIRECT IF

2. Diseases caused by Rickettsiae typhus group:a) BRILL ZINSER DSEb) BOUTONNEUSE FEVERc) MURINE /ENDEMICd) SCRUB TYPHUS

3. Causative agent/s for rickettsia spotted fever group:a) R. rickettsib) R. tsutsugamishic) R. prowazekid) R. akari

4. Microbial features of B-hemolytic group A streptococci:a) Erythrogenic toxinsb) L-fucosyl transferasec) Hyaluronidased) Galactosyl transferase


3/4

5. Using the rantz-randall test for ASO titer determination:a) The red cell control must not display hemolysisb) The erythrocyte suspension is composed of human O RBC at 5% suspensionc) The Streptolysin O ctrl must display complete hemolysisd) The erythrocyte suspension is composed of rabbit erythrocytes

6. In the WIDAL test rapid slide method:a) Makes use of the kline slideb) Rotates at 180 rpm for 5 minutesc) Gently shake the commercially prepared salmonella antigens to ensure heterozygous

suspension

d) A serum volume of 0.08 mL is approximately a 1:20 dilution7. Serologic features of Myco. pneumonia:

a) Cold hemagglutininsb) Heterophile antibody production which cross reacts with capsular polysaccharide of

Strep. Pyo group

c) Cross reactive antigensd) Allo antibody production

8. For Q fever:a) The area of occurrence is Europe, Africab) The etiologic agent is Coxiella burnettic) Its reservoir hosts are mites and rodentsd) Its mode of transmission is via recrudescence

9. Rules in the performance of febrile agglutinin test:a) Tests are preferably done every 6-12 daysb) Definite diagnosis is by 2-3 daysc) Antibodies are occasionally formed by related infectious groupsd) Battery test is not advocated for brucella and rickettsia spp.

NON ARE CORRECT

10. In heterophile antibody test for IM:a) In Forsman Ab: with GPK, antibodies are absorbed. Hence no agglutination with sRBCb) In serum sickness: with GPK, antibodies are not absorbed. Hence agglutination with

sRBC.

c) In IM: with GPK, antibodies are not absorbed. Hence agglutination with sRBC.d) In serum sickness: with BE, antibodies are not absorbed. Hence agglutination with sRBC.

11.For cold hemagglutinins in Prim. Atyp. Pneumoniaa) It appears during the 1st week of infectionb) It reaches its peaks on 1st to 5th week of infectionc) Declines to low levels on the 7th week

12.A heterophile antibody test for rickettsia: WEIL FELIX TEST13.During paratyphoid fever antibody titer reaches its peak within how many weeks? 3-4 WEEKS14.Significant titer for vaccinated persons? 16015.Flagellar antigen: H antigen


4/4

16.Unit used for ASO rapis slide latex: IU/mL17.For cold hemagglutinin test for PAP, the highest dilution that shows a 2+ result is considered a

positive result. FALSE, WRONG WORD=2+, RIGHT WORD=1+

18. In Ehrlichioses, the mode of transmission for E. canis is the lone star tick bite. TRUE19. In tularaemia infection, antibodies usually appear within 3-5 weeks. FALSE, WRONG WORD=3-5,

RIGHT WORD=2

20.What does CEDIA mean? CLONE ENZYME DONOR IMMUNO ASSAY21.Significant titer for unvaccinated persons using the WIDAL RAPID SLIDE TEST? 8022.4 points: Give 4 methods used to determine Rheumatoid factor in rheumatoid arthritis

Rose-waaler Ziff Modification by heller Euglobin hemagglutination

1. Also known as progressive systemic sclerosis: SCLERODERMA2. ANA pattern that is seen in progressive systemic sclerosis and sjogrens syndrome? NUCLEOLAR

PATTERNS

3. Also known as heavy chain disease? FRANKLINS DISEASE/MALIGNANT LYMPHOMA4. Causes hyperthyroidism due to thyroid stimulating immunoglobulin: GRAVES DISEASE5. Primary hypothyroidism: HASHIMOTOS THYROIDITIS6. Autoimmune disease due to impaired T cell production: DIGEORGE SYNDROME7. Autoimmune disease caused by a defect in the maturation of B cells: BRUTONS DISEASE8. Highly associated to the presence of immunoglobulin light chains in the urine: MULTIPLE

MYELOMA9. Breast cancer associated antigen: CA 15-310.Tumor marker for pancreatic cancer: CA 19-911.4 POINTS: Give 4 types of hypersensitivity

Basophil mediated hypersensitivity Antibody mediated cytotoxicity hypersensitivity Immune complex hypersensitivity Delayed type hypersensitivity

12.AFP is a major fetal protein that resembles globulin. FALSE, WRONG WORD=GLOBULIN, RIGHTWORD=ALBUMIN

13.CA 125 is a high molecular weight purine- like glycoprotein useful for non mucinous epithelialovarian cancer FALSE, WRONG WORD=PURINE, RIGHT WORD=MUCIN

Documents

Serology Questions 100 Points With Answers