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Supporting Information
A simple and efficient Fluorophoric probe for dual
sensing of Fe3+ and F-: Application to bioimaging
in native cellular iron pool and live cell
Chirantan Kara, Soham Samantaa, Sandipan Mukherjeeb, Barun K. Duttaa, Aiyagari
Ramesh*b and Gopal Das*a
a Department of Chemistry, Indian Institute of Technology Guwahati, Assam, 781 039, India.
b Department of Biotechnology, Indian Institute of Technology Guwahati, Assam, 781 039,
India.
E-mail: [email protected]; [email protected]
Fax: +91-361-258-2349; Tel: +91-361-258-2313
Electronic Supplementary Material (ESI) for New Journal of Chemistry.This journal is © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2014
Figure S1: Job’s plot between L1 and Fe3+ ions. Where Xhost = the mole fraction of L1 and ΔI is the change (I-I0) in the intensity of the emission spectra in presence of guest.
Figure S2: Mass spectrum of L1-Fe complex.
Figure S3: Bensei-Hildebrand plot obtained from the fluorescence emission (calculated at λem) studies.
Figure S4: Changes of the of fluorescence emission of compound L2 (10 μM) observed upon addition of 10 eqv. of Fe3+ ions.
Figure S5: Selected orbitals and their energies for L1 at B3LYP/6-31G(d).
Figure S6: Optimized proposed structures of L1 and L1-Fe complex
Figure S7: Coordinates for optimized geometry of L1 at B3LYP/6-31G(d,p).
Figure S8: Coordinates for optimized geometry of L1 at B3LYP/6-31G(d,p)
Figure S9: fluorescence emission (calculated at λem) of L1 on addition of increasing concentration of Fe3+ in different protein environment.
Figure S10: Mass spectrum of L1-Fe+Fluoride
Figure S11: 1H NMR spectrum of L1 in DMSO-d6 solution.
Figure S12: 1H NMR spectrum of L1 in DMSO-d6 solution (expanded aromatic part).
Figure S13: 13C NMR spectrum of L1 in DMSO-d6 solution.
Figure S14: Mass spectrum of L1 (Mass spectrum obtained in positive mode).
Figure S15: Changes in fluorescence intensity of L1-Fe complex in present of different amounts of proteins. A) metHb B) FBS C)HAS
References:
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