“Formation Mechanism of monodisperse, LMW Chitosan

nanoparticles by Ion gelation technique”

N.D.M.V.P. Samaj’s College of Pharmacy, Gangapur Road, Nasik 422 002

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Presented by- Under the

guidance of-NITIN P. KANWALE Dr. M.H. Bele

Department of QUALITY ASSURANCE TECHNIQUES

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Contents- -Introduction.

- Materials and methods.

- Characterization.

-Results and discussions.

-Inferences

-References

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Objective- To solve the problem of chitosan/TPP nanoparticles with high degree of monodispersity & stability.

 

Introduction

Why Monodisperse system?

Why low molecular weight chitosan is is taken as a? polymer

Why ion gelation technique is used for the formation?

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Chitosan

It is a linear polysaccharide composed of randomly distributed ß-(1-4)- linked D- glucosamine and N-Acetyl D-glucosamine.

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Materials and Methods

Mixing FILTER

Filter Filter

LMW Chitosan +Acetic acid solution

Stirred with magnetic stirrer for overnight

PH adjusted to4.7-4.8

Pass through syringe filter

TPP + ULTRA PURE WATER

Pass through syringe filter(0.22µm)

Addition of the both solution under the specific Conditions

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Characterization

Particle size (HPPS-5000) Particle size distribution

Polydispersity Index

Morphological characterization. (HR TEM)

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Results and discussions

1.Effect of the concentration on both solution-

Size of the particles get increased with increase in concentration.

under acidic condition there is electrostatic repulsion b/w chitosan molecules.

Found that the chitosan under the low con. Could form

stable nanoparticles even at a low mass ratio, while could form only stable nanoparticles at higher conc.

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2. Effect of mass ratio-

Fig: Effect of the mass ratio of chitosan to TPP on particle size And zeta potential by adding different volumes of TPP to 10ml of Chitosan solution

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3. Effect of PH of chitosan solution

The effect of the pH of chitosan solution on the formation of chitosan/TPP particles was investigated by adjusting the pH from 3.6 to 5.5 (3.6, 4.0, 4.2, 4.5, 4.7, 5.0, 5.2, 5.5).

The results indicated that the critical mass ratio of chitosan to TPP for the formation of an opalescent suspension decreased with the pH decreasing.

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4. Effect of Temperature of chitosan solution

Fig.- Effect of temperature of solution on particle size.

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5. Effect of the ambient temperature during cross- linking

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6. Effect of the concentration of acetic acid

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7. Effect of the Stirring speed

The particle size distribution was significantly narrowed by increasing stirring speed from 200 rpm to 800 rpm.

8. Colloidal StabilityEvaluated by storage at room temperature (10–20◦C) for different

periods of time.

The results showed that after stored at room temperature for 20 days, the liquid was still a clear suspension with light blue opalescence and the nanoparticles did not show any statistically significant change in particle size and its distribution during the investigation.

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Inference- Decreased toxicity

Compatibility with drugs

Good carrier for delivery of drugs

Increased sustained release action

No problem with solubility

Easy to form by this method

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References-

1. Wen Fana, Wei Yanb, Zushun Xub, et al “Formation mechanism of monodisperse, low molecular weight chitosan nanoparticles by ionic gelation technique” Colloids and Surfaces B: Biointerfaces 90: (2012):21– 27.

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