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• SCI.9-12.B-3.4 - [Indicator] - Summarize how the structures of organic molecules (including proteins, carbohydrates, and fats) are related to their relative caloric values.
• SCI.9-12.B-3.5 - [Indicator] - Summarize the functions of proteins, carbohydrates, and fats in the human body.
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*Organic *Organic CompoundsCompounds
• CompoundsCompounds that contain CARBONCARBON are called organicorganic.
• MacromoleculesMacromolecules are large organic moleculesorganic molecules.
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*Carbon (C)*Carbon (C)• CarbonCarbon has 4 electrons4 electrons in
outer shell.
Usually bonds with C, H, O or C, H, O or NN.
• Example:Example: CHCH44(methane)(methane)
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*Macromolecules*Macromolecules
• Large organic molecules.Large organic molecules.• Also called POLYMERSPOLYMERS.• Made up of smaller “building
blocks” called MONOMERSMONOMERS.• Examples:Examples:
1. Carbohydrates1. Carbohydrates2. Lipids2. Lipids3. Proteins3. Proteins4. Nucleic acids (DNA and RNA)4. Nucleic acids (DNA and RNA)
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*Question:*Question:How Are How Are
MacromolecMacromolecules ules
Formed?Formed?copyright cmassengale
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*Answer:*Answer: Dehydration Dehydration SynthesisSynthesis
• Also called “condensation “condensation reaction”reaction”
• Forms polymerspolymers by combining monomersmonomers by “removing “removing water”water”.
HO H
HO HO HH
H2O
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*Question:*Question: How are How are
Macromolecules Macromolecules separated or separated or
digested?digested?
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*Answer: *Answer: HydrolysisHydrolysis
•Separates monomersmonomers by “adding water”“adding water”
HO HO HH
HO H
H2O
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• SCI.9-12.B-3.4 - [Indicator] - Summarize how the structures of organic molecules (including proteins, carbohydrates, and fats) are related to their relative caloric values.
• SCI.9-12.B-3.5 - [Indicator] - Summarize the functions of proteins, carbohydrates, and fats in the human body.
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CarbohydratCarbohydrateses
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*I. Carbohydrates*I. Carbohydrates
• Small sugar moleculesSmall sugar molecules to large sugar moleculeslarge sugar molecules.
• Examples:Examples:A.A. monosaccharidemonosaccharideB.B. disaccharidedisaccharideC.C. polysaccharidepolysaccharide
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*Carbohydrates*CarbohydratesMonosaccharide: one sugar Monosaccharide: one sugar
unitunit
Examples:Examples: **glucose (glucose (C6H12O6)
deoxyribosedeoxyribose
riboseribose
FructoseFructose
GalactoseGalactose
glucoseglucose
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*Carbohydrates*CarbohydratesDisaccharide: two sugar unitDisaccharide: two sugar unit
Examples: Examples: – *Sucrose (glucose+fructose) *Sucrose (glucose+fructose)
table sugartable sugar– *Lactose (glucose+galactose)*Lactose (glucose+galactose)– Maltose (glucose+glucose)Maltose (glucose+glucose)
glucoseglucoseglucoseglucose
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*Carbohydrates*CarbohydratesPolysaccharide: many sugar unitsPolysaccharide: many sugar units
Examples:Examples: starch (bread, starch (bread, potatoes)potatoes)
glycogen (beef glycogen (beef muscle)muscle)
cellulose (lettuce, cellulose (lettuce, corn)corn)
glucoseglucoseglucoseglucose
glucoseglucoseglucoseglucose
glucoseglucoseglucoseglucose
glucoseglucoseglucoseglucose
cellulosecellulose
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• SCI.9-12.B-3.4 - [Indicator] - Summarize how the structures of organic molecules (including proteins, carbohydrates, and fats) are related to their relative caloric values.
• SCI.9-12.B-3.5 - [Indicator] - Summarize the functions of proteins, carbohydrates, and fats in the human body.
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*II. Proteins *II. Proteins • Amino acids (20 different kinds of aa)
bonded together by peptide bondspeptide bonds (polypeptidespolypeptides). Essential and non-essential
• Six functions of proteins:Six functions of proteins:1.1. Storage:Storage: albumin (egg white)albumin (egg white)2.2. Transport: Transport: hemoglobinhemoglobin3.3. Regulatory:Regulatory: hormoneshormones4.4. Movement:Movement: musclesmuscles5.5. Structural:Structural: membranes, hair, nailsmembranes, hair, nails6.6. Enzymes:Enzymes: cellular reactionscellular reactions
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• Amino acids– Are organic molecules possessing both
carboxyl and amino groups– Differ in their properties due to differing side
chains, called R groups
Twenty Amino Acids
• 20 different amino acids make up proteins
O
O–
H
H3N+ C C
O
O–
H
CH3
H3N+ C
H
C
O
O–
CH3 CH3
CH3
C C
O
O–
H
H3N+
CH
CH3
CH2
C
H
H3N+
CH3CH3
CH2
CH
C
H
H3N+
C
CH3
CH2
CH2
CH3N+
H
C
O
O–
CH2
CH3N+
H
C
O
O–
CH2
NH
H
C
O
O–
H3N+ C
CH2
H2C
H2N C
CH2
H
C
Nonpolar
Glycine (Gly) Alanine (Ala) Valine (Val) Leucine (Leu) Isoleucine (Ile)
Methionine (Met) Phenylalanine (Phe)
C
O
O–
Tryptophan (Trp) Proline (Pro)
H3C
Figure 5.17
S
O
O–
O–
OH
CH2
C C
H
H3N+
O
O–
H3N+
OH CH3
CH
C C
HO–
O
SH
CH2
C
H
H3N+ C
O
O–
H3N+
C C
CH2
OH
H H H
H3N+
NH2
CH2
OC
C CO
O–
NH2 O
C
CH2
CH2
C CH3N
+
O
O–
O
Polar
Electricallycharged
–O O
C
CH2
C CH3N
+
H
O
O–
O– O
C
CH2
C CH3N
+
H
O
O–
CH2
CH2
CH2
CH2
NH3+
CH2
C CH3N
+
H
O
O–
NH2
C NH2+
CH2
CH2
CH2
C CH3N
+
H
O
O–
CH2
NH+
NHCH2
C CH3N
+
H
O
O–
Serine (Ser) Threonine (Thr)Cysteine
(Cys)Tyrosine
(Tyr)Asparagine
(Asn)Glutamine
(Gln)
Acidic Basic
Aspartic acid (Asp)
Glutamic acid (Glu)
Lysine (Lys) Arginine (Arg) Histidine (His)
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*Primary Structure
Amino acids bonded together by peptide peptide bonds (straight chains)bonds (straight chains)
aa1 aa2 aa3 aa4 aa5 aa6
Peptide Bonds
Amino Acids (aa)
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Proteins Proteins (Polypeptides)(Polypeptides)
Four levels of protein Four levels of protein structure:structure:
A.A. Primary StructurePrimary Structure
B.B. Secondary Structure Secondary Structure
C.C. Tertiary Structure Tertiary Structure
D.D. Quaternary Structure Quaternary Structure
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Secondary StructureSecondary Structure
• 3-dimensional folding arrangement of a primary primary structurestructure into coilscoils and pleatspleats held together by hydrogen bondshydrogen bonds.
• Two examples:Two examples:
Alpha HelixAlpha Helix
Beta Pleated SheetBeta Pleated Sheet
Hydrogen BondsHydrogen Bondscopyright cmassengale
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Tertiary StructureTertiary Structure• Secondary structuresSecondary structures bentbent and
foldedfolded into a more complex 3-D more complex 3-D arrangementarrangement of linked polypeptides
• Bonds: H-bonds, ionic, disulfide Bonds: H-bonds, ionic, disulfide bridges (S-S)bridges (S-S)
• Call a “subunit”.“subunit”.
Alpha HelixAlpha Helix
Beta Pleated SheetBeta Pleated Sheetcopyright cmassengale
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Quaternary Quaternary StructureStructure
•Composed of 2 or more “subunits”•Globular in shape•Form in Aqueous environments•Example: enzymes (hemoglobin)enzymes (hemoglobin)
subunitssubunits
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• SCI.9-12.B-3.4 - [Indicator] - Summarize how the structures of organic molecules (including proteins, carbohydrates, and fats) are related to their relative caloric values.
• SCI.9-12.B-3.5 - [Indicator] - Summarize the functions of proteins, carbohydrates, and fats in the human body.
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*Proteins must be in a certain shape
to function. If you take it out of its shape, you have denatured it and it can not longer work. Heat and pH can denature a protein.
Proteins often change colors when they are denatured. Cooking egg white is an example.
•Denaturation is when a protein unravels and loses its native conformation(shape)
Denaturation
Renaturation
Denatured protein
Normal protein
Figure 5.22
*2 types of amino acids• Non-essential amino acids are those
your body can make. There are 12.• Essential amino acids are those you
must get in your diet because your body cannot make them. There are 8.
• If you don’t get the essential aa, you develop kwashiorkor.
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Review of Protein Structure
+H3NAmino end
Amino acidsubunits
helix
• SCI.9-12.B-3.4 - [Indicator] - Summarize how the structures of organic molecules (including proteins, carbohydrates, and fats) are related to their relative caloric values.
• SCI.9-12.B-3.5 - [Indicator] - Summarize the functions of proteins, carbohydrates, and fats in the human body.
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Enzyme reactions
enzyme + substrate enzyme-substrate complex
*Enzymes -- Are defined as a BIOLOGICAL catalyst i.e. something that speeds up a reaction. Up to 1012 fold– *Usually end in ‘…ase’ and named for
what they do or act on (sucrase breaks down sucrose)
– Discovered in 1900 in yeasts. Some 40,000 in human cells
– *Control almost every metabolic reaction in living organisms
– Are globular proteins coiled into a very precise 3-dimentional shape with hydrophilic side chains making them soluble
– Possess an active site into which other substrate molecules can bind to form an enzyme-substrate complex
– *Once the substrate has been either synthesised or split, enzymes can be re-used.
– Do not ‘create’ reactions– Widely used in industrial cleaning– Often require co-factors (co-enzymes)
to function – metal ions, or vitaminsSUCROSE IS SUBSTRATE – SUCRASE IS ENZYME
Enzyme reactions
enzyme + substrate enzyme-substrate complex
E +S ES
Enzyme reactions
enzyme + productenzyme-substrate complex
E +PES
enzyme + substrate enzyme-substrate complex
E +S ES
*Enzyme activity
How fast an enzyme is workingRate of Reaction
Rate of Reaction = Amount of substrate changed (or amount product formed) in a given period of time.
Rate
of
React
ion
Enzyme activity
Variable you are looking at
*Enzyme activity
Four Variables
*Enzyme activity
Four Variables
TemperaturepH
Enzyme Concentration
Substrate Concentration
Rate
of
React
ion
Temperature
Rate
of
React
ion
Temperature
0 20 30 5010 40 60
Rate
of
React
ion
Temperature
0 20 30 5010 40 60
40oC - denatures
5- 40oC Increase in Activity
<5oC - inactive
*Effect of heat on enzyme activty
If you heat the protein above its optimal temperature, bonds break and the protein loses it secondary and tertiary structure.
Cooking an egg denatures the proteins and it goes white. Cooking meat denatures the proteins and it turns brown.
Effect of heat on enzyme activty
Denaturing the protein
Effect of heat on enzyme activty
Denaturing the protein
ACTIVE SITE CHANGES SHAPE SO SUBSTRATE NO LONGER FITS
Rate
of
React
ion
pH
1 3 42 5 6 7 8 9
Rate
of
React
ion
pH
1 3 42 5 6 7 8 9
Narrow pH optima
Rate
of
React
ion
pH
1 3 42 5 6 7 8 9
Narrow pH optima
WHY?
Rate
of
React
ion
pH
1 3 42 5 6 7 8 9
Narrow pH optima
Disrupt Ionic bonds - Structure
Effect charged residues at activesite
• SCI.9-12.B-3.4 - [Indicator] - Summarize how the structures of organic molecules (including proteins, carbohydrates, and fats) are related to their relative caloric values.
• SCI.9-12.B-3.5 - [Indicator] - Summarize the functions of proteins, carbohydrates, and fats in the human body.
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*III. Lipids*III. Lipids• General term for compounds which are not not
soluble in watersoluble in water.• Lipids are soluble in hydrophobic solventsare soluble in hydrophobic solvents.• Remember:Remember: “stores the most energy”“stores the most energy”• Examples:Examples: 1. Fats1. Fats
2. Phospholipids2. Phospholipids3. Oils3. Oils4. Waxes4. Waxes5. Steroid hormones5. Steroid hormones6. Triglycerides6. Triglycerides
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*Lipids*LipidsSix functions of lipids:Six functions of lipids:
1.1. Long term Long term energy storageenergy storage2.2. Protection against heat loss Protection against heat loss (insulation)(insulation)3.3. Protection against physical shockProtection against physical shock4.4. Protection against water lossProtection against water loss5.5. Chemical messengers (hormones)Chemical messengers (hormones)6.6. Major component of membranes Major component of membranes ((phospholipids)phospholipids)
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*Fatty Acids*Fatty AcidsThere are two kinds of fatty acidsfatty acids you may see these on
food labels:
1.1. Saturated fatty acids:Saturated fatty acids: no double bonds (bad) no double bonds (bad)
2.2. Unsaturated fatty acids:Unsaturated fatty acids: double bonds (good) double bonds (good)O
C-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH3
=
saturatedsaturated
O
C-CH2-CH2-CH2-CH=CH-CH2 -CH
2 -CH2 -CH
2 -CH3
=
unsaturated
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*Lipids*LipidsTriglycerides:Triglycerides:
ccomposed of 1 glycerol1 glycerol and 3 3 fatty acidsfatty acids.
H
H-C----O
H-C----O
H-C----O
H
glycerol
O
C-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH3
=
fatty acids
O
C-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH3
=
O
C-CH2-CH2-CH2-CH =CH-CH2 -CH
2 -CH2 -CH
2 -CH3
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*Steroid
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Steroids are always four fused rings.Cholesterol is the precursor for all steroid hormones.•Testosterone•Estrogen
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*IV. Nucleic acids*IV. Nucleic acids• Two types:Two types:
a. Deoxyribonucleic acid a. Deoxyribonucleic acid (DNA-(DNA- double helix) double helix) b. Ribonucleic acid (RNA-single b. Ribonucleic acid (RNA-single strand) strand)
• Nucleic acids Nucleic acids are composed of long chains of nucleotidesnucleotides linked by dehydration synthesisdehydration synthesis.
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*Nucleic acids*Nucleic acids• Nucleotides include:Nucleotides include:
phosphate groupphosphate grouppentose sugar (5-carbon)pentose sugar (5-carbon)nitrogenous bases:nitrogenous bases:
adenine (A)adenine (A)thymine (T) DNA onlythymine (T) DNA onlyuracil (U) RNA onlyuracil (U) RNA onlycytosine (C)cytosine (C)guanine (G)guanine (G)
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NucleotideNucleotide
OO=P-O O
PhosphatePhosphate GroupGroup
NNitrogenous baseNitrogenous base (A, G, C, or T)(A, G, C, or T)
CH2
O
C1C4
C3 C2
5
SugarSugar(deoxyribose)(deoxyribose)
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*Nucleotides are bonded to each other by the process of dehydration synthesis, forming phosphodiester bonds.
The arrows in the next slide are pointing to these bonds.
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DNA - double helixDNA - double helix
P
P
P
O
O
O
1
23
4
5
5
3
3
5
P
P
PO
O
O
1
2 3
4
5
5
3
5
3
G C
T A
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