Embed Size (px)
Citation preview
How to contact us:Where to Order
Informationand Assistance
Internet Address
Contact any authorized J.T.Baker distributor or Mallinckrodt Baker +31-570-687-500
Call +31-570-687-500 for Customer Service and Assistance
http://www.jtbaker.com
EUROPEAN HEADQUARTERS: Mallinckrodt Baker B.V. Teugseweg 20, P.O. Box 1, 7400 AA, Deventer, The Netherlands Tel: +31-570-687500; Fax: +31-570-687574E-mail: [email protected]
FRANCE: Mallinckrodt Baker, France33 Avenue Philippe Auguste, 75011, Paris, FranceTel: +33-1-44-64-89-71/2; Fax: +33-1-44-64-89-73 E-mail: [email protected]
GERMANY: Mallinckrodt Baker, DeutschlandIm Leuschnerpark 4, 64347 Griesheim, GermanyTel: +49-6155-667939; Fax: +49-6155-667940E-mail: [email protected]
ITALY: Mallinckrodt Baker, ItaliaVia P. Portaluppi 11/2, 20138 Milano, ItalyTel: +39-02-580891; Fax: +39-02-58019264E-mail: [email protected]
UNITED KINGDOM: Mallinckrodt Baker, UK107/112 Leadenhall Street, London EC3A 4AH, United KingdomTel: +44-1908-506000; Fax: +44-1908-503290E-mail: [email protected]
NORTH AMERICA: Mallinckrodt Baker, Inc.222 Red School Lane, Philipsburg, NJ 08865, USATel: 800-582-2537 within U.S.; 908-859-2151E-mail: via www.solvitcenter.com
MEXICO: Mallinckrodt Baker S.A. de C.V.Col. Lindavista, Deleg. Gustavo A. Madero, 07300 Mexico D.F., MEXICOTel: 52-555-569-1100 Fax: 52-555-755-2585E-mail:[email protected]
MALAYSIA: Mallinckrodt Baker Sdn. Bhd.26 Jalan SS26/13, 47301 Petaling Yaha, Selangor, MALAYSIATel: (603) 78030378 Fax: (603) 78030405E-mail: [email protected]
SINGAPORE: Mallinckrodt Baker, Inc. c/o Tyco Healthcare Pte. Ltd. No. 26, Ang Mo Kio Industrial Park 2, #04-01, 569507 SINGAPORETel: 656-4820 100 Fax: 656-4820-300
When every step counts
SamplePreparation
www.jtbaker.com
C-1
0/10
.000
010
4
BAKERBOND™, Speedisk™, ULTRA RESI-ANALYZED™, BAKER ANALYZED™, CYCLE-TAINER™, the J.T.Baker logo and J.T.Baker® are all trademarks of Mallinckrodt Baker, Inc.
2
J.T.BakerYour professional guide through Solid Phase Extraction
3
IntroductionAmong chromatography suppliers,J.T.Baker stands out with a uniquebreadth of separations capabilities.You may know us for our high purityreagents, but we have also been inthe Liquid Chromatography (LC)business since the 1970s. In fact, wepioneered the development of SolidPhase Extraction (SPE).
We’re manufacturers of chromato-graphic media, developers of proprietary bonding chemistry, andproviders of products and know-howfor diverse separations techniques,scale and applications. Our productsfor analytical separations are focusedin three distinct applications:
• Analytical HPLC - WithBAKERBOND™ media for largemolecule or small molecule separations available in columnsor as bulk media.
• Flash Media and Cartridges - Our new, high-performancespherical silica media can befunctionalized with a variety ofsurface chemistries for optimalapplication performance.
• Solid Phase Extraction(both BAKERBONDconventional columnsand Speedisk™ high performance columns)- Our new 96-Wellplate, optimal for auto-
mated liquid handling systems, isthe most recent addition to theSpeedisk family. Our Speediskcolumns perform 9 times fasterthan conventional SPE columns.
Solid Phase Extraction for Sample PreparationWe are never satisfied with the status quo. Our improved BAKER-BOND spe products provide standard,reliable performance for today’sresearch, as well as QC pharma-ceutical, clinical and environmentalapplications. Our extraction tubedesign is optimized for SPE, andcompatible with manual and auto-mated SPE column processors. Ourproprietary column component formulations are ultraclean.
Furthermore, a wide range of BAKERBONDsorbents let you rationally choose thefunctionality that’sappropriate for yoursample.
The purpose of sample preparation
Sample clean up Necessary to eliminate impuritiesand/or isolate the component ofinterest from the matrix. This helpsto increase lifetime of the analyticalcolumn, prevents contamination ofequipment, thereby protectingexpensive instrumentation.
Sample concentration in order to reach the detection limits of the analytical equipment.Sample preparation selectively concentrates the components ofinterest prior to analysis.
Product characteristics
BAKERBOND spe Columns
With BAKERBOND spe columns,you can choose the solid phaseextraction column that best fitsyour sample size and performancerequirements. Use standardBAKERBOND spe 1, 3 and 6 mlultraclean polypropylene and glasscolumns, packed with 50-3000 mgsorbent, for samples from 0.2 to 10 ml
when standard speed, recovery, andfinal concentration with good economy are needed. • Optimized, round-rimmed tube
and frits made from ultrapurepolymers.
• Tubes fit into all popular processors and racks.
• Multi phase columns for specialenvironmental applications (e.g.PAH’s, PCB’s and Pesticides)
• Mixed Mode narc™ columns fordrugs of abuse sample preparation
• Wide Pore (300 Å) sorbents forbiological applications.
BAKERBOND Speedisk columns
J.T.Baker Speedisk columns reduceSPE run times by 60-80% and mayeliminate other time consumingsample preparation steps, such aspre-filtration and evaporation, some-times required with conventional SPEcolumns.
Thanks to the unique patentedSpeedisk laminar design andBAKERBOND high performancemicro-particles you not only getshorter run times, but also higher
capacity, optimum recovery andmore effective separation. The designis especially useful in clinical, biologicaland pharmaceutical drug applications.Speedisk columns are disposable,ultraclean, polypropylene columns(1-6 ml), pre-packed with high performance micro particles of 10µm(10-200 mg) and available in a widerange of sorbents (20 silica basedand 6 polymer based) to meet allyour separation needs.
‘Method Development Kits’ areavailable for BAKERBOND spe andSpeedisk columns. Please contact yourlocal office for more information.
Speedisk 96 Columns and Speedisk 96-well plates
BAKER spe- 12G Column Processor
Speedisk 96 columns multiply theadvantages of Speedisk columns by 96 to raise high throughput SPEto a higher level of performance. We offer these Speedisk products intwo different geometries. For methoddevelopment we offer the versatilemodular Speedisk 96 columns. Weinsert 96 “rimless” 20 mg Speediskcolumns in the Speedisk 96 columnholder and the assembly is ready forplacement on the Speedisk 96Positive Pressure Processor.
For detailed information on our separation programme please visit
www.jtbaker.com/chromatography
Screen
Guard Filter
Microparticulate Sorbent
Support Filter
Screen
4 5
Fast, innovative & reliableSample preparation with J.T.Baker SPE technology
You can remove unneeded columnsfrom the holder, and assemble customtrays for efficient method develop-ment. Once method development iscomplete, use the Speedisk 96-wellplate, a one-piece, molded plate thatis pre-assembled with the sorbent ofyour choice. The new Speedisk 96-wellplate was designed with standardgeometries to adapt to popular auto-mated liquid handling systems.
Choose from more than 20 functiona-lities of silica and polymer-based sorbents for either Speedisk 96 columnsor the new Speedisk 96-well plates.
Positive Pressure Processors andAccessories for Extraction Columns
Speedisk 48 and 96 Positive Pressure Processor and
Speedisk 96 Column Holder
Speedisk 96 Positive Pressure Processor:The Speedisk Positive PressureProcessor enables you to combinethe advantages of a positive displace-ment processor with Speedisk columns.The processor produces the sameflow rate in each of the 96 columnsarrayed in 96 well plate.
Speedisk 48 Positive Pressure Processor:The Speedisk Positive PressureProcessor is perfect for processing 1,3 and 6 ml columns in batches of 1-48 samples. It distributes N2 orcompressed air to displace liquidfrom BAKERBOND spe columns or BAKERBOND Speedisk columnsand it easily enables a high controland precision.
Accessories for Extraction Columns
BAKER spe- 12G Column Processor
Mallinckrodt Baker also offers a widerange of accessories for your separationtechnology. Please go to our website,www.jtbaker.com/chromatography andselect accessories.
BAKERBOND SpeediskExtraction DisksThe correct choice for water samplesfrom 200 ml to 2 L. Our patenteddisk is pre-assembled for use inpreparing aqueous samples for analysis.Its laminar configuration provides
filtration capacity so it can easilyhandle samples with high particulates.
The BAKERBOND Speedisk designresists clogging and ensures highthroughput rates even when samplescontain solids. Flow rate, capacity,recovery, and precision are high dueto the unique disk configuration and performance of BAKERBOND sorbent.
Comparison between BAKERBOND spe columns and BAKERBONDSpeedisk columns:
General product overview
For detailed information on our separation programme please visit
www.jtbaker.com/chromatography
BAKERBONDspe column
BAKERBONDSpeedisk column
BAKERBONDSpeedisk 96 well plates
BAKERBONDSpeediskextraction disk
Sample preparation step BAKERBOND spe columns BAKERBOND Speedisk columns
Column Size / Sorbent 1 cc / 100 mg 1 cc / 20 mg
Particle Size 40 µm 10 µm
Sample Volume 2 ml 1 ml
Column conditioning 2 ml (20-40 sec) 0.5 ml (5-10 sec)
Sample addition 2 ml (100 sec) 50 ul-0,5 ml (50 sec)
Washing 1.5 ml (15-20 sec) 0.4 ml (2-5 sec)
Elution 1-2 ml (15-20 sec) 0,3-0,6 ml (5-10 sec)
Sample concentration / evaporation 3-10 minutes reduced or eliminated
Silica based SPE products
• BAKERBOND specolumns:reverse phasenormal phaseion exchangeadsorptiondrug of abuse
• BAKERBOND Speediskcolumns:reverse phasenormal phaseion exchangeadsorptiondrug of abuse
• BAKERBOND Speedisk 96 Silica Columns (in holder)
• BAKERBOND Speediskextraction disks:for manual extraction stations and for automated extractors.
Polymer based SPE products
• Styrene Divinylbenzene copolymer (SDB) BAKERBOND spe columns.
• Speedisk Divinylbenzene (DVB) columnsH2O-Philic DVBH2O-Philic SC-DVBH2O-Phobic DVBH2O-Phobic SC-DVBH2O-Philic SA-DVBH2O-Phobic WA-DVB
• BAKERBOND Speedisk 96 Polymer Columns (in holder)
• BAKERBOND Speedisk 96-Well Plates Polymer Sorbents (compatible with automatic systems)
• BAKERBOND Speedisk extraction disks:for manual extraction stations and for automated extractors
6 7
J.T.BakerYour professional guide through Solid Phase Extraction
If your free sample isn’there, please contact our customer service
department
For trouble free analysis, we recommend the following product lines andproduct information out of the J.T.Baker programme:
• ULTRA RESI-ANALYZED solvents• BAKER ANALYZED HPLC-Reagents solvents• BAKER ANALYZED LC-MS reagents• BAKERBOND HPLC columns for various applications• BAKERBOND Speedisk Column Instructions• Solid Phase Extraction - Technical guide• CYCLE-TAINER™ solvent delivery system• Cross reference lists available on the website• Application Notes
Selection Guide for spe sorbents and solventsORGANIC SAMPLES MW< 2000 (in solution)
This Sorbent Selection Guide is a systematic guide classifying samples according to polarity, ionizability and solubility in water or organic solvents. This information is useful in
selecting the necessary components of a preliminary extraction method
Sample Organic Solvent Soluble Water Soluble Solubility
Ionic Non-ionic/Ion-paired
Matrix Organic Organic Aqueous Aqueous Aqueous Aqueous Aqueoussample
Polar Moderately Polar Non Polar Anionic Cationic Non Polar Moderately Polar
Polar
SeparationMechanism1 NPC LSC RPC IEC IEC RPC LSC NPC
SPE Phase H2O-Philic DVB H2O-Phobic DVB H2O-Phobic DVB H2O-Phobic H2O-Phobic SA-DVB H2O-Phobic SC-DVB H2O-Phobic DVB H2O-Philic DVBrecomm.2 Cyano H2O-Philic DVB H2O-Philic DVB H2O-Philic DVB H2O-Philic SA-DVB H2O-Philic SC-DVB H2O-Philic DVB Cyano
Diol Silica gel SDB-1/SDB-2 Amino Cyano SDB-1/SDB-2 Silicag gel Diol
Amino Florisil Octadecyl 1,2 Amino Carboxylic Acid Octadecyl Florisil Amino
1,2 Amino Alumina Octyl Quaternary Amine Sulfonic Acid Octyl Alumina 1,2 Amino
Cyclohexyl Cyclohexyl
Phenyl Phenyl
Cyano Cyano
Solvents3,4 Hexane Hexane Hexane Acids, buffers Acid, bases, buffers Hexane Hexane Hexane
Chloroform Chloroform Dichloromethane Dichloromethane Chloroform Chloroform
Dichloromethane Dichloromethane Acetone Acetone Dichloromethane Dichloromethane
Acetone Ethyl acetate Acetonitrile Acetonitrile Ethyl acetate Acetone
Methanol Methanol Methanol Methanol Methanol Methanol
Water Water
1Separation Mechanisms 2Bonded phases listed in order of increasing polarity
LSC: Liquid Solid Chromatography (Adsorption) 3Eluting solvents listed in order of increasing polarity
NPC: Normal Phase Chromatography (Bonded Phase Partition) 4Selective elution can be performed by combining two or more miscible solvents
RPC: Reversed Phase Chromatography (Bonded Phase Partition) to achieve various degrees of polarity
IEC: Ion-Exchange Chromatography (Bonded Phase Ion-Exchange)
3,4 Solvents:
9262 Hexanes (95% n-hexane), ULTRA RESI-ANALYZED
9257 Chloroform, ULTRA RESI-ANALYZED
SDB: styrene divinyl benzene 9264 Dichloromethane, ULTRA RESI-ANALYZED
DVB: divinyl benzene polymer 9260 Ethyl acetate, ULTRA RESI-ANALYZED
H2O-Phobic WA DVB: Weak anion exchanger 9254 Acetone, ULTRA RESI-ANALYZED
H2O-Phobic SC DVB: Strong cation exchanger 9255 Acetonitrile, ULTRA RESI-ANALYZED
H2O-Philic SA DVB Strong anion exchanger 9077 Methanol , ULTRA RESI-ANALYZED
H2O-Philic SC DVB: Strong cation exchanger 4219 Water, ULTRA RESI-ANALYZED
This Sorbent Selection Guide is also available on: www.jtbaker.com/chromatography/SolidPhaseExtraction.asp
For detailed information on our separation programme please visit
www.jtbaker.com/chromatography
