Salk Institute Mobile Lab Gel Electrophoresis Student Instructions Edvotek set up Salk Institute Mobile Lab

Salk Institute Mobile Lab

Gel Electrophoresis

Student Instructions

Edvotek set up



Gel Electrophoresis Student Station Set-up

tip container w/tips

waste container

5µl fixed pipettor

practice sheet in protector

foam epi rack w/ 1 food color tube each: orange, green, purple


How to Use a 5µl fixed pipettor

• Make sure a yellow tip is firmly twisted onto the end of the red pipettor

• Carefully open one of the food color tubes – Don’t let the lid snap back

and splash you!

• Hold down the button on the top of the pipettor



• While holding the button down, insert the tip into the food color tube



• Release the button and remove from the food color tube

• The tip now contains just 5 microliters of solution!



• To dispense the solution push down on the button again

• Using your other hand to guide the end of the tip, practice dispensing the 5µl drops – Try to not push down on

the plastic sheet - see if you can hold the pipettor tip just above the paper



• Twist off the dirty tip into the waste container and replace with a clean tip

• Always use a clean tip each time!!



• Ready to load a real gel?



• Load the food color solutions into the wells on your group’s gel

• Be careful not to poke through the bottom of the wells!

Documents

Salk Institute Mobile Lab Gel Electrophoresis Student Instructions Edvotek set up Salk Institute Mobile Lab