Result:

Calculations:

Buffer dilution:

M 1V 1=M 2V 2(275ml)(1 g/ml)=(50 g/ml)(V 2ml)V 2=5.5mlbuffer

Amount of distilled water: (275- 5.5)ml = 269.5ml of distilled water needed

Therefore, only 5.5ml amount of buffer need to be added to 269.5ml of distilled water to

produce 275ml of 1g/ml TAE buffer solution.