International journal of Applied Pharmaceutical and Biological Research, 2016

FORMULATION AFORMULATION AFORMULATION AFORMULATION A

L K

T. John College of Pharmacy,

ABSTRACT

There is a considerable amount of attention being given to herbal formulationa lower or zero side effects when compared to the synthetic formulations available in the market. The objective of the present study involves the preparation of a herbal oil using coconut oil, olive oil and Tulsi extract and ion hair follicle and its antimicrobial activity. The preparation was also subjected to various tests for analysis including moisture content, total ash, acid insoluble ash, water soluble ash, water insoluble ash and sulphated ash. Other physical tests were also conducted like viscosity, surface tension, pH, acid value, etc. and were compared with some synthetic marketed formulations. The herbal hair oil prepared is on par with the marketed ones in quality and in some cases showing better activity marketed formulations which may lead to adverse reactions like hypersensitivity, but our preparation contains no such synthetic preservatives and utilizes the natural antimicrobial activity of herbs for the extension of shelf

Keywords: Herbal hair oil, Coconut oil, Olive oil, T

Hair oils are those preparations compiled for hair care and may aid in the prevention and treatment of baldness and or other hair related ailments. Coconut oil has used since time immemorial to promote the luxurious growth of hair. Olive oil is such another oil used to enhance hair growth by improving its overall health as it has been found to condition, moisturize and even prevent DTH (dihydrotestosterone) production to large extent. But such oiproperly, they may lead to fatty acid deposition inespecially in the facial area, which may lead to bacterial growth which is one of the main reasons for blackheads and other undesirable formations. Tubacterial properties. This study involves the preparation of a hair oil to increase hair growth at the same time maintain germicidal properties. The bactericidal property is also applied to displace the need of any synthetic preservatives

Preparation: The hair oil was prepared by mixing a definite ratio of coconut oil and olive oil. It was also fortified with Vitamin E. Tulsi (Ocimum sanctum) extract was supplemented to

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International journal of Applied Pharmaceutical and Biological Research, 2016

Research ArticleResearch ArticleResearch ArticleResearch Article ISSN : 2456

165

FORMULATION AFORMULATION AFORMULATION AFORMULATION AND EVALUATION OF HERND EVALUATION OF HERND EVALUATION OF HERND EVALUATION OF HERBAL HAIR OILBAL HAIR OILBAL HAIR OILBAL HAIR OIL

L K Bisht, B Jacob, M Menon*

T. John College of Pharmacy, Gottigere, Bangalore, Karnataka

There is a considerable amount of attention being given to herbal formulation nowadays a lower or zero side effects when compared to the synthetic formulations available in the market. The objective of the present study involves the preparation of a herbal oil using coconut oil, olive oil and Tulsi extract and ion hair follicle and its antimicrobial activity. The preparation was also subjected to various tests for analysis including moisture content, total ash, acid insoluble ash, water soluble ash, water insoluble ash and sulphated ash.

tests were also conducted like viscosity, surface tension, pH, acid value, etc. and were compared with some synthetic marketed formulations. The herbal hair oil prepared is on par with the marketed ones in quality and in some cases showing better activity and tolerance. Synthetic preservatives were found to be usedmarketed formulations which may lead to adverse reactions like hypersensitivity, but our preparation contains no such synthetic preservatives and utilizes the natural antimicrobial activity of herbs for the extension of shelf

il, Coconut oil, Olive oil, Tulsi extract.

INTRODUCTION

Hair oils are those preparations compiled for hair care and may aid in the prevention and treatment of baldness and or other hair related ailments. Coconut oil has used since time

promote the luxurious growth of hair. Olive oil is such another oil used to enhance hair growth by improving its overall health as it has been found to condition, moisturize and even prevent DTH (dihydrotestosterone) production to large extent. But such oi

lead to fatty acid deposition in the scalp and increase comodogenicity, especially in the facial area, which may lead to bacterial growth which is one of the main reasons for blackheads and other undesirable formations. Tulsi has been shown to possess excellent antibacterial properties. This study involves the preparation of a hair oil to increase hair growth at the same time maintain germicidal properties. The bactericidal property is also applied to

any synthetic preservatives1-6.

MATERIAL AND METHODS

The hair oil was prepared by mixing a definite ratio of coconut oil and olive oil. It was also fortified with Vitamin E. Tulsi (Ocimum sanctum) extract was supplemented to

International journal of Applied Pharmaceutical and Biological Research, 2016; 1(2):165-167

ISSN : 2456-0189

BAL HAIR OILBAL HAIR OILBAL HAIR OILBAL HAIR OIL

Karnataka, India

due to a better activity and a lower or zero side effects when compared to the synthetic formulations available in the market. The objective of the present study involves the preparation of a herbal oil using coconut oil, olive oil and Tulsi extract and its effect on hair follicle and its antimicrobial activity. The preparation was also subjected to various tests for analysis including moisture content, total ash, acid insoluble ash, water soluble ash, water insoluble ash and sulphated ash.

tests were also conducted like viscosity, surface tension, pH, acid value, etc. and were compared with some synthetic marketed formulations. The herbal hair oil prepared is on par with the marketed ones in quality and

reservatives were found to be used in many of the marketed formulations which may lead to adverse reactions like hypersensitivity, but our preparation contains no such synthetic preservatives and utilizes the natural antimicrobial activity of herbs for the extension of shelf life.

Hair oils are those preparations compiled for hair care and may aid in the prevention and treatment of baldness and or other hair related ailments. Coconut oil has used since time

promote the luxurious growth of hair. Olive oil is such another oil used to enhance hair growth by improving its overall health as it has been found to condition, moisturize and even prevent DTH (dihydrotestosterone) production to large extent. But such oils if not used

the scalp and increase comodogenicity, especially in the facial area, which may lead to bacterial growth which is one of the main reasons

lsi has been shown to possess excellent anti-bacterial properties. This study involves the preparation of a hair oil to increase hair growth at the same time maintain germicidal properties. The bactericidal property is also applied to

The hair oil was prepared by mixing a definite ratio of coconut oil and olive oil. It was also fortified with Vitamin E. Tulsi (Ocimum sanctum) extract was supplemented to

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M. Menon et al

enhance antimicrobial activity. The formulation was tested for its physicochemical properties and the results are displayed. till dissolved completely. The resulting solution was stirred coVitamin E was mixed well with it. Jasmine and rose perfumes were also added for a better aromatic appeal. The resulting mixture was further stirred for another 5 minutes

Table 1: Physico-chemical Evaluation by compar

Sl.No. Sample *

1 Prepared formulation

2 A3 B4 C5 D

Table 2: Evaluation of Antimicrobial property by measuring the Zone of Inhibition

Sl.No.

1

2

*The fungus mentioned in Aspergillus niger and the E.

Figure 1: Herbs Used

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IJAPBR, 2016; 1(2):165

166

enhance antimicrobial activity. The formulation was tested for its physicochemical properties and the results are displayed. 10ml of coconut oil was dissolved in 20 ml of olive oil and heated till dissolved completely. The resulting solution was stirred continuously for 15 minutes. 5 ml of Vitamin E was mixed well with it. Jasmine and rose perfumes were also added for a better aromatic appeal. The resulting mixture was further stirred for another 5 minutes

chemical Evaluation by comparison with Marketed Preparations

Sample * Acid Value

pH Viscosity (cP)

Prepared formulation

2.68 6.2 30.0120

A 2.24 6 34.480 B 3.36 5.5 31.035 C 7.01 6 21.670 D 4.76 6.1 32.480

Evaluation of Antimicrobial property by measuring the Zone of Inhibition

Sl.No. Sample Zone of Inhibition

Bacteruim* Fungus*

Pure tulsi oil 14+_0.6 12+_0.5

Prepared

formulation

10+_0.4 9+_0.5

*The fungus mentioned in Aspergillus niger and the E. coli is the bacterium used.

Figure 1: Herbs Used and Culture Media Prepared

IJAPBR, 2016; 1(2):165-167

enhance antimicrobial activity. The formulation was tested for its physicochemical properties 10ml of coconut oil was dissolved in 20 ml of olive oil and heated

ntinuously for 15 minutes. 5 ml of Vitamin E was mixed well with it. Jasmine and rose perfumes were also added for a better aromatic appeal. The resulting mixture was further stirred for another 5 minutes7-10.

ison with Marketed Preparations

Density (mg/ml)

0.980

1.075 0.810 0.860 1.056

Evaluation of Antimicrobial property by measuring the Zone of Inhibition

coli is the bacterium used.

and Culture Media Prepared

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M. Menon et al IJAPBR, 2016; 1(2):165-167

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RESULTS & DISCUSSIONS

The pH and Acid value of the prepared formulation was found to be similar to the marketed preparations. The viscosity and density was also found to be satisfactory for use. As shown, the hair oil possessed sufficient antibacterial and anti-fungal properties, which increases its potential to combat seborrhea and acne.

CONCLUSION

The herbal oil prepared was found to be a safe product with its pH being in normal range. Acid value was also found to be satisfactory. The antimicrobial properties was sufficiently exhibited. The formulated product was devoid of any potentially irritant synthetic substances like parabens as it consisted entirely of natural substances. Thus the formulation was superior to the marketed brands due to itsof non-irritancy, safety and due to the complete absence of any artificial ingredients.

REFERENCE

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2) Indian Pharmacopoeia, 2014 3) Sunita Bansod and Mahendra Rai. Antifungal Activity of Essential Oils from Indian

Medicinal Plants Against Human Pathogenic Aspergillus fumigatus and A. niger. World Journal of Medical Sciences 2008;3(2),81-88

4) Soliman KM: Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi. Food Chem Toxicol: 2008;40(11); 1669-75

5) Romagnoli CR: Chemical characterization and antifungal activity of essential oil of capitula from wild Indian Tagetes patulaL. Protoplasma. 2005; 57-65

6) Hoffman DL: The Herb User’s Guide Wellingborough UK Thorsons Publishing group. 7) Janssen AM: Antimicrobial activity of essential oils. Aspects of the test methods. Planta

Medica: 1987;53, 395-398 8) Smith MD: A new protocol for antimicrobial testing of oils: J. Microbiol. Methods: 1997;

28,21-24 9) Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews.

1999;12,564-582. 10) Ahmad H, Khan SM, Ghafoor S, Ali N. Ethnobotanical study of upper Siren. Journal of

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