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Recombinant Technology for ND and MD Vaccine strains
Early Protection…. Always Producing More
Fowl
Jungle Fowl
Domestication
Backyard
Fowl
Commercial Farm
Modern Farm
21 days 50 days
19802020
Cycle of 71 days… 30% hatchery… 70% farms
Cycle of 56 days… 38% hatchery …62% farms
35 days
Cycle of 71 days… 30% hatchery… 70% farms
Genetics : Broiler
Genetics : Layer
Chick 17 week 72 week 100 week.
330 488.5
Body weight (Broiler )
4.5 TIMES.50 TIMES.
Chick 1 Week 35 Days
40 GM 180 GM 2 KG
• Housing
• Feed
• Climate control
• Disease control
Hatchery
High quality Hatching eggHealthy parentsVaccination program for MDA
• Housing
• Feed
• Climate control
• Disease control
Biosecurity
Monitoring
Vaccination
KEEP BROILER PRODUCTION EFFICIENT
Optimize the processes
Ease the processes
Safeguard performance
Do as much as possible in the hatchery
hatchery vaccination- (s/c, in ovo)
•Equipment to standardize, less labor• Minimum and early reaction• No disturbance during growing period
MOVE VACCINATION AS
MUCH AS POSSIBLE INTO
THE HATCHERY
• Fewer hatcheries than farms ( Hatchery Vs Farms )
• Easier to control standardized procedures (SOP )
-Preparation and administration
• (semi) automatic equipment
-standardize, uniform application, reduce human factor
• Create uniform conditions
Easier to control temperature, relative humidity and light
• Introduction of new Hatchery vaccines
VaccinationEarly protection built from the hatchery
Vaccination
Vaccination Options
Conventional Method
Hatchery Vaccination
In ovo Vaccination
Farm level
Hatchery level
Hatchery level
Vaccination
Vaccination options
In ovo D18
Subcutaneous injection
At hatch
Spray/eye drop
Introduction of new Hatchery vaccines
Innovating with Science
Leading with Prevention
Advancing Well-being
Recombinant vaccines
• Recombinant vaccines are based on new technologies of molecular engineering
• It is all based on the technology of the recombination of the DNA (genetic engineering)
Recombinant vaccineswe have single construct and dual construct vaccine on the market
• Consists of a vector (virus or bacteria) expressing foreign antigens
• Genes that codify for 1 or more antigens (proteins) are introduced in the vector’s genome
• The immune response (cellular & humoral) is against the vector, including the inserted antigen(s), without the need of using the causative agent
2 in 1Marek + ND protection
Innovax-ND
2 in 1!
Nobilis ND CL30
HVT
HVT
ILTpart of ND virus
F-gene of ND Cl30
HVT
rHVT+ND/F
molecular engineeringConstruction of recombinant
Herpes virus
NDV disease of poultry
ND SB1 first generation“single construct”
rHVT+ND/F
Recombinant Innovax NDcombine 2 in 1
HVT
HVT
ILT
ND Cl30
Existing vaccines
Marek protection
Newcastle disease
protection
NDV - disease of poultry
Herpes virus
3/2/2020
How does it work
3/2/2020
Expression of F gene in NDVresponsible for F-protein on virus surface
F Gene
F Gene
ND VirusF Protein
F Protein
AttachmentHost cell
NDV Infection
3/2/2020
Mode of Action F Protein NDV:
Initiates the infection of host cell
Chicken cell
F protein fuses with receptor cell membrane
ND Virus F-protein
NDV Infection
3/2/2020
Insertion of the F gene of NDV in HVT genome
rHVT/F “Recombinant”
INNOVAX-ND single constuct
F gene + promoter & terminator
inserted in HVT viral genome
HVT Virus
ND virus
Construction of recombinant
3/2/2020
Mode of Action INNOVAX-ND Vaccine(HVT/F)
F protein
Foreign protein
Production
F antibody
HVT/F Recombinant replication
F geneHVT Glycoproteins
inducing immunity
against MD
3/2/2020
INNOVAX®-ND-SB1
The HVT part will still induce marek protection up
to the level of related to HVT
The F-protein will induce F-antibodies and
The F antibodies will neutralize the F protein of
ND virus “blocks” (prevents) the infection of the
chicken after a NDV field exposure.
3/2/2020
Action of INNOVAX-ND SB1 Vaccine
Chicken cell
F antibody
ND virus
3/2/2020
Action of INNOVAX-ND SB1
Chicken cell
F antibody
ND virus
Antibodies block attachment
3/2/2020Presentation Name
INNOVAX®-ND +SB1
Is a combination of HVT (ND )+SB1
To improve mareks protection up to the level of HVT+SB1
INNOVAX®-ND
Is a combination of HVT +ND
Advantages of a recombinant vaccine
• Absence of post vaccination reaction
• No interference with ND MDA or other vaccines
• No dissemination of vaccine virus
• Convenient and homogenous administration (in-ovo or day-old hatchery)
• Act as a marker vaccine (DIVA: Differentiate Infected from Vaccinated Animals )
• Ease vaccination program 2 in 1
Recombinant Vaccines: Characteristics
• Development of immunity depends of the replication of the vector
• Need of priming with conventional ND vaccines in high challenge areas
• Serological monitoring is different
Efficacy
Innovax ND-SB1
Challenge study ND
Protection from 2 weeks of age increasing tofull protection* by 4 weeks of age
MD challenge with vvMDV(RB1B)
5th Day – 94% Protection
0
20
40
60
80
100
11 15 21
% P
rote
cti
on
Age (days)
Development of protection after in ovo vaccination of SPF birds with Innovax-ND
Challenge studies with vvNDV strains from different genotypes and geographic areas
USA: Texas GB – Genotype II
Mexico: Chimalhuacan – Genotype V
Thailand: CU2 – Genotype VII
UK: Herts – Genotype IV
For our new dual construct (InnovaxND-IBD) in addition we also showed ND protection against GenotypeVII from Egypt and Belgium
As reference the Hitchner B1 and LaSota strains belong to genotype II
Challenge study ND
3 in 1Marek +ND+IBD protection
Innovax- ND-IBD
3 in 1!Marexine CA
Vector
HVT
ILT
Immunogenic parts of ND virus
F gene
HVTrHVT-F-VP2
Herpes virus
ILT
ND-IBD second generation“Double construct”
ILTF surface glycoprotein
Immunogenic parts of IBD virus
VP2 gene VP2 glycoprotein
NDCl30
Donor F gene
Faraghar
Donor VP2
Marek & Recombinant vaccine storageCell associated vaccines (CA)
LN2 (-196 C)
CA vaccines are frozen vaccines and should be stored under LN
Presentation title /35
•
Preparation of CA vaccines training as it is one of the major steps for the success
4 Key Points- Preparation
Needle Size – 18 G ( while mixing after thawing )
Thawing Time - 90-120 sec
Thawing Temp – 27 °C
Administration Time – 45 Min
work in hygienic way prevent contamination
37
In ovoDay-old Sub-cutaneous injection
RecombinantHatchery vaccination
Perfect fit in:
Innovating with Science
First dual recombinant vaccine ever in Animal Health
Revolutionary technology to control main viral diseases in poultry
Leading with Prevention
Hatchery vaccine solution to ND, IBD and ILT
Improved performance early vaccination
Advancing Well-being
No vaccine reaction
Reduced diseases incidence leading to less use of antibiotics
Recombinant Vaccines
Summary
Technology
Products
Automation
Thank you for saving my life
WVPA (India) ConferenceAdvances in Poultry Science for One Health
28th February 2020, Bangalore – Indiafor Membership and other info please Log
into
www.wvpaindiaconference.com
www.wvpa.inor email to : [email protected]