Recombinant DNA• Restriction enzymes

& palindromes • Cloning with

plasmid vectors• Restriction mapping

& RFLPs• Probes• PCR analysis &

technique • DNA sequencing

Restriction enzymes & palindromes

Cloning with plasmid vectors

Cloning

Cloning

Restriction mapping & RFLPs

Probes

Detection using probes

ASO= allele specific oligonucleotidesCan detect single nucleotide change

Sickle cell anemiaCystic Fibrosis

PCR analysis & technique

PCR applications

DNA sequencing

DNA sequencing

Transgenic plants

Gene Therapy