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Genomics & DNA sequences
•Satellite DNA: tandem repeats near centromere, heteochromatin•Microsatellite: dinucleotide repeats•VNTR: variable number of tandem repeats (mini-satellites)•SINES (<500 bp, 500,000X in genome) and LINES (6400 bp, 100,000X),transposable
Recombinant DNA• RFLP• Restriction enzymes & palindromes • Probes • Restriction mapping • Use of Vectors • Cloning with plasmid vectors • Site directed mutagenesis • Constructing DNA libraries • PCR analysis & technique • DNA sequencing
Detection using probes
ASO= allele specific oligonucleotidesCan detect single nucleotide change
Sickle cell anemiaCystic Fibrosis
Pedigree analysis
Allele A = 5 kbAllele B = 2 kb On different chromosomes
III-1= received A from father And B from mother
III-2 received A from motherAnd B from father
III-3 received B from both Parents
Taken together mutant alleleWith B,