RECITATION #2 CHAPTER 14 LODISH & WP Chaperone · PDF fileFigure 14.11 Vescle-mediated protein trafficking between the ER and cis-Golgi. ... Lodish et al. Figure 14.18 Structure of

Copyright © 2013 by W. H. Freeman and Company Molecular Cell Biology, 7th Edition Lodish et al.

RECITATION #2 CHAPTER 14 LODISH &

WP Chaperone paper


Figure 14.1

Overview of the secretory and endocytic pathways of protein sorting.

Proteins that are for

secretory vescicles,

lysosome or having

posted receptors on pm

than will bind specific

ligands for endocyotic

vescicles to bring them

into cell and digest them

in an endosome.



Figure 13.24 Protein import into the mitochondrial matrix.


Experimental Figure 13.25 Experiments with chimeric proteins elucidate mitochondrial protein

import.


Figure 13.27 Three pathways to the inner mitochondrial membrane from the cytosol.


Experimental Figure 14.2 Protein transport through the secretory pathway can be visualized by

fluorescence microscopy of cells producing a GFP-tagged membrane protein.




Figure 14.6 Overview of vesicle budding and fusion with a target membrane.



Figure 14.8 Model for the role of Sar1 in the assembly and disassembly of COPII coats.



Figure 14.10 Model for docking and fusion of transport vesicles with their target membranes.


Figure 14.11 Vescle-mediated protein trafficking between the ER and cis-Golgi.


Figure 14.12

Three-dimensional structure of the ternary complex comprising the COPII coat proteins Sec23 and Sec24 and Sar1·GTP.

Sar1 is the locator that

binds Sec23 /24

forming the coat

complex.


Figure 14.13 Role of the KDEL receptor in retrieval of ER-resident luminal proteins from the Golgi.


Figure 14.14 Processing of N-linked oligosaccharide chains on glycoproteins within cis-, medial-, and trans-Golgi

cisternae in vertebrate cells.


Experimental Figure 14.15 Electron micrograph of the Golgi complex in an exocrine pancreatic cell reveals

secretory and retrograde transport vesicles.


Figure 14.17 Vesicle-mediated protein trafficking from the trans-Golgi network.


Figure 14.18 Structure of clathrin coats.


Figure 14.19 Model for dynamin-mediated pinching off of clathrin/AP-coated vesicles.


Experimental Figure 14.20 GTP hydrolysis by dynamin is required for pinching off of clathrin-coated

vesicles in cell-free extracts.


Figure 14.21 Formation of mannose 6-phosphate (M6P) residues that target soluble enzymes to

lysosomes.


Figure 14.22

• Newly synthesized

protein for the lysosome

acquire a man 6PO4

residue, which is

necessary to pakg into

vescile for lysosome

Trafficking of soluble lysosomal enzymes from the trans-Golgi network and cell surface to lysosomes.


Experimental Figure 14.23 Proteolytic cleavage of proinsulin occurs in secretory vesicles after they

have budded from the trans-Golgi network.


Figure 14.24 Proteolytic processing of proproteins in the constitutive and regulated secretion

pathways.


Figure 14.25 Sorting of proteins destined for the apical and basolateral plasma membranes of

polarized cells.


Experimental Figure 14.26 The initial stages of receptor-mediated endocytosis of low-density

lipoprotein (LDL) particles are revealed by electron microscopy.


Figure 14.27 Model of low-density lipoprotein (LDL).


Experimental Figure 14.28 Pulse-chase experiment demonstrates precursor-product relations in

cellular uptake of LDL.


Figure 14.29 Endocytic pathway for internalizing low-density lipoprotein (LDL).


Figure 14.30 Model for pH-dependent binding of LDL particles by the LDL receptor.

• This is the pH

dependent LDL receptor

for lipoprotien particles

carrying FA and

cholesterol to cells that

display the LDL

recerptor on its cell

membrane.


Figure 14.31 The transferrin cycle, which operates in all growing mammalian cells.

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RECITATION #2 CHAPTER 14 LODISH & WP Chaperone · PDF fileFigure 14.11 Vescle-mediated protein trafficking between the ER and cis-Golgi. ... Lodish et al. Figure 14.18 Structure of