RAS oncogene

Research goals

Outline

RAS oncogene

Molecular characterization

Mammalian counterpart

Point mutations

Transforming oncogene

Biochemical properties

Ras family

Ras GDP-GTP Cycle

Ras membrane localization

Ras signaling network

Ras mutations

Mutation spectrum

GTP hydrolysis

Oncogenic driver

Neoplastic transformation

Ras signaling

Ras membrane association

Ras inhibition

Direct inhibition

Kinase inhibitors

MAPK pathway

MAPK pathway

Feedback activation

MEKi combinations

Therapeutic window

Synthetic lethality

Oncogene addiction

Synthetic lethal interactions

Dissecting the contribution

siRNA platform

KRAS addiction

Ras effector and stress response

Single node dependency

Private node dependency

Paired node dependency

Paired node pair dependencies

Private node pair dependency

Gene paralog combinations

Deconvolution

Knockdown

Cell cycle arrest

Autophagy pathways

RAF kinase and autophagy

Co-targeting

MAPK and autophagy

Acknowledgment